Preparation of Sedoheptulose and Aldose by Immobilized Enzyme Cascade Reaction

A technology of sedoheptulose and erythrulose, applied in the direction of immobilized enzymes, biochemical equipment and methods, enzymes, etc., can solve the problems of expensive raw materials, low product purity, and low reaction efficiency
CN108277244BActive Publication Date: 2020-09-08SHENZHEN READLINE BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN READLINE BIOTECH CO LTD
Publication Date
2020-09-08

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Abstract

The invention relates to a preparation method of sedoheptulose and aldose in an immobilized enzyme cascade reaction, and specifically discloses a method for preparing sedoheptulose and aldose in the immobilized enzyme cascade reaction. The method comprises the following steps: taking L-erythrulose and 1,3-dioxyacetone as starting materials, and preparing D-sedoheptulose-7-phosphoric acid by usinga one-pot method of immobilized kinase EK, immobilized isomerase TRI, erythrulose phosphoric acid isomerase RPI, immobilized aldolase FSA and immobilized ATP regenerated enzyme PPK; and further generating sedoheptulose and aldose. The method is high in preparation efficiency and cheap in raw materials, and is applicable to large-scale industrial production, so that the preparation cost is greatlyreduced.
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Description

technical field

[0001] The invention relates to a preparation method of sedoheptulose and aldose, in particular to a method for preparing sedoheptulose and aldose by using immobilized enzyme cascade reaction. Background technique

[0002] Sedoheptulose jing-tian geng ketone sugar / aldose is a monosaccharide with molecular formula C 7 h 14 o 7 , The molecular weight is 210. Sedoheptulose was discovered by LaForge and Hudson in Sedum spectabile, a plant of Sedum spectabile. It is one of the few seven-carbon sugars in nature. It is an important primary and secondary metabolite in the synthesis of shikimic acid and aromatic ammonia. Although a large number of documents have reported the important physiological functions of sedoheptulose / aldose in organisms, the high price has seriously restricted the further application and promotion of this seven-carbon sugar; It becomes very important to reduce the application cost of heptulose / aldose method.

[0003] The conventional pre...

Claims

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