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Diagnosis and treatment of mers-related renal disease

A nephropathy and related technology, applied in the field of diagnosis and treatment of MERS-related nephropathy, can solve the problems of mortality, unknown pathogenic mechanism, and difficulty in specific treatment.

Active Publication Date: 2018-07-17
VERSITECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, the pathogenic mechanism of kidney injury associated with MERS infection, which is a significant contributor to mortality, is unknown and thus not readily amenable to specific treatment

Method used

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  • Diagnosis and treatment of mers-related renal disease
  • Diagnosis and treatment of mers-related renal disease
  • Diagnosis and treatment of mers-related renal disease

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Experimental program
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Embodiment

[0078] Virus : SARS-CoV strain HKU39849 and MERS-CoV isolate HCoV-EMC / 2012 by Ron Fouchier 27 supply. Viruses were grown in VeroE6 cells (obtained in Dulbecco's Modified Eagle's Medium (DMEM)) supplemented with 10% fetal calf serum (FCS) and 100 units / ml penicillin + 100 μg / ml streptomycin (1% PS) Breeding from ATCC). All studies were performed according to Biosafety Level 3 practices.

[0079] by TCID 50 Experimental virus titration: The 50% tissue culture infectious dose (TCID) of MERS-CoV and SARS-CoV in VeroE6 cells was determined by observing the cytopathic effect (CPE). 50 ) / ml. Briefly, cells were 5 x 10 in 150 μl of DMEM 4 Cells / well density were plated in 96-well plates. virus in DMEM from 10 3 to 10 14 Serial dilutions were made by half log. One hundred microliters of each dilution was added per well; and the plates were observed daily for 5 consecutive days for CPE.

[0080] cell culture : HK-2 cells (obtained from ATCC) were cultured in DMEM / F12 med...

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Abstract

Methods for treating, diagnosing, and providing prognostic information for kidney disease related to MERS-CoV infection are described. Transcription and translation products (for example, mRNA or peptides) of FGF2, Smad7, and / or sequences upstream of FGF2 are useful as markers that indicate kidney disease and / or provide prognostic information related to the course of kidney disease related to MERS-CoV infection. Such kidney disease can be treated and / or mitigated by reducing the availability of FGF2, Smad7, and / or a sequence upstream from FGF2 by reducing and / or regulating transcription and bysequestering related peptides using a large molecule binding partner, such as an antibody. Sequences for useful siRNAs and antisense oligonucleotides are provided.

Description

[0001] This application claims the benefit of US Provisional Application No. 62 / 170,780, filed June 4, 2015. These and all other cited external materials are hereby incorporated by reference in their entirety. To the extent that the definition or use of a term in a reference incorporated by reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein controls. technical field [0002] The field of the invention is the diagnosis and treatment of diseases and disorders associated with coronavirus infections, especially MERS. Background technique [0003] The Background Description includes information that may be useful in understanding the present invention. It is not an admission that any of the information presented herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art. [0004] Middle East respiratory syndrome coron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K31/7088A61K39/395A61K39/00
CPCC12Q1/6883C12Q2600/112C12Q2600/118C12Q2600/158C12Q1/70A61K31/7105A61K31/713C07K16/22C07K2317/76G01N2333/165G01N2333/4703G01N2333/50G01N2800/347G01N2800/52A61P11/00A61K31/7088A61K39/00G01N33/68
Inventor 杨文龙陈福和刘耀南袁国勇
Owner VERSITECH LTD
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