The method and application of predicting the outcome of blood diseases by using the red blood cell dna damage signal
A signal prediction and red blood cell technology, applied in the field of biomedicine, can solve the problems of long detection cycle, unfavorable improvement of patient diagnosis and treatment and long-term prognosis, and low sensitivity
Active Publication Date: 2020-07-24
杭州太铭生物科技有限公司
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[0004] At present, the diagnosis of acute leukemia is to eliminate as many leukemia cell populations as possible and control the massive proliferation of leukemia cells, and relieve various clinical manifestations caused by leukemia infiltration, in order to obtain complete remission. Diagnosis and treatment methods are also increasingly developed and improved. The diagnosis and risk stratification of acute leukemia are mainly determined based on the test results of cell morphology, immunology, cytogenetics and molecular typing. However, due to the high cost of testing, many patients only Perform simple morphological examination, which is unfavorable for the improvement of patient diagnosis and long-term prognosis
Of course, there are still methods on the market to predict the development and prognosis of patients with acute leukemia by detecting the levels of lactate dehydrogenase (LDH) and serum ferritin (SF) by enzyme reaction rate method and reflex immunoassay, as well as by RT- PCR method is used to monitor the expression of blood cell phosphatase (SHP-1) gene and proto-oncogene c-kit as an indicator of leukemia outcome, but these conversion prediction methods have poor specificity, low sensitivity, cumbersome steps, and long detection cycle, so they still cannot Well adapted for clinical treatment and diagnosis
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[0031] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.
[0032] Those skilled in the art should understand that in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention and The above terms should not be con...
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A method for predicting prognosis of a blood disease by using erythrocyte DNA damage signals, comprising the following steps: S1, preparation of a blood sample; S2, preparation of a control group and an experimental group; S3, staining of markers and nucleic acid of the experimental group; S4, re-suspension of the control group; S5, detection based on a flow-type method; S6, obtaining of a result; and S7, analysis of the result. The prognosis of a blood disease is predicted by quickly determining DNA damage signals in early damaged erythrocyte, and therefore, the method has high specificity and sensitivity.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular to a method for predicting the outcome of blood diseases by using red blood cell DNA damage signals and its application, which is widely used in clinical medicine. Background technique [0002] Hematological diseases, also known as hematopoietic system diseases, refer to diseases that originate in the hematopoietic system, or diseases that affect the hematopoietic system accompanied by abnormal blood changes. The hematopoietic system includes blood, bone marrow mononuclear system, macrophage system and lymphatic tissue. In a nutshell, any disease that involves the pathology and physiology of the hematopoietic system and takes it as the main manifestation belongs to the category of blood diseases. Clinically, blood diseases can be divided into red blood cell diseases, white blood cell diseases, bleeding and thrombotic diseases, specifically, leukemia, aplastic anemia, myelodysplastic synd...
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IPC IPC(8): G01N15/00G01N15/10G01N15/14
CPCG01N15/00G01N15/10G01N15/14G01N15/1434G01N2015/0069G01N2015/1006
Inventor 高晓飞张清葛建萍楼赛
Owner 杭州太铭生物科技有限公司