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A kind of mouse fibroblast tumor cell line and application thereof

A technology of fibroblasts and tumor cell lines, applied in the field of biomedicine, can solve the problems of cell-free, reports, and leukemia-free fibroblast tumors.

Active Publication Date: 2021-05-11
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leukemia-associated fibroblasts were first reported in BLOOD magazine in September 2019. At present, there are no reports on leukemia-associated fibroblast tumors and tumor-associated fibroblast tumors at home and abroad, and there are no reports on related cell lines

Method used

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  • A kind of mouse fibroblast tumor cell line and application thereof
  • A kind of mouse fibroblast tumor cell line and application thereof
  • A kind of mouse fibroblast tumor cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example relates to the isolation and establishment of mouse fibroblast tumor cell line HXWMF-1.

[0031] (1) Human acute lymphoblastic leukemia cells HXEX-ALL1 in the logarithmic growth phase were divided into 5×10 6 Each / 100µl / mouse, inoculated subcutaneously in the nude area of ​​female Balb / c (nu / nu) mice (5 weeks old, weighing 14-16g); observe the tumor formation at the inoculation site every other day, and the tumor volume was about 920 mm 10 days after inoculation 3 , take tumor block to make cell suspension; press 5×10 5 Cells / ml density were resuspended in RPMI 1640 medium (Gibco) containing 10% fetal bovine serum (Thermo) at 37°C, saturated humidity, 5% CO 2 Incubator cultivation. After 3 days, the growth of adherent cells can be seen. Remove the supernatant and suspension cells, and change the medium every 3 to 5 days. After 24 days, the morphology of the adherent cells is relatively uniform, and the cell proliferation is accelerated after 40 days of in...

Embodiment 2

[0039] This example relates to the identification of the growth and biological characteristics of the mouse fibroblast tumor cell line HXWMF-1.

[0040] (1) Observation of cell morphology:

[0041] Take the HXWMF-1 cells in the logarithmic growth phase and observe the living cell morphology under an inverted microscope, as shown in figure 1 As shown, the cells grow adherently, the shape is relatively uniform, fusiform or irregular triangular, the ratio of nucleus to plasma is inverted, the nucleolus is clear, no contact inhibition, can overlap and grow, and the cells gather in clusters when they are dense; routine slide fixation, Switzerland - Gimusa staining, observe the cell morphology under an upright microscope, such as figure 2 As shown, the cells are spindle-shaped, with a large nuclear-to-cytoplasmic ratio, cleavage phases, round nuclei, and multiple nucleoli.

[0042] (2) Growth curve and doubling time determination:

[0043] Collect the cells in logarithmic growth...

Embodiment 3

[0051] This example relates to the establishment of a fibroblast tumor animal model of the cell line HXWMF-1.

[0052] Tumor formation experiment in nude mice: HXWMF-1 cells in the logarithmic growth phase were divided into 3×10 6 Each / 100µl / mouse, inoculated subcutaneously in the nude area of ​​female Balb / c (nu / nu) mice (5 weeks old, weighing 14-16g); observe the tumor formation at the inoculation site every other day, and the results are as follows: Figure 6 and Figure 7 As shown, two days after inoculation, the size of rice grains could be touched under the skin of nude mice. Five days after inoculation, the tumor grew rapidly. The size of the tumor was measured the next day. It can be seen that the volume of the tumor increased from 161 mm on the fifth day. 3 2835 mm grown to day 15 3 15 days later, the tumor block was taken, and part of the tissue was fixed with paraformaldehyde, embedded in paraffin, sectioned, and stained with HE (see Figure 8 ) showed that the t...

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Abstract

The invention discloses a mouse fibroblast tumor cell line and its application. The cell line is a leukemia-related mouse fibroblast tumor cell line established for the first time at home and abroad. The cell name is mouse fibroblast tumor cell line HXWMF‑1, with a deposit number of CCTCC NO: C2019244, can be used for various applications such as establishing mouse fibroblast tumor animal models.

Description

technical field [0001] The invention relates to a mouse fibroblast tumor cell line and application thereof, specifically relates to leukemia-related mouse fibroblast tumor cell line HXWMF-1 and application thereof, and belongs to the technical field of biomedicine. Background technique [0002] Fibroblasts, also called fibroblasts, are the main cellular components of loose connective tissue. Tumor-associated fibroblasts (Tumor-associated fibroblasts, TAFs) are activated fibroblasts in the tumor microenvironment. TAFs secrete a variety of growth factors and cytokines, such as VEGE, HGF, TGFβ, IL-6, IL-8, CCL2, CCL5, CXCL9 and PGE2, etc., which provide a favorable microenvironment for tumor growth and promote tumor growth, metastasis, induction Angiogenesis is even involved in the induction of tumor drug resistance, affecting patient prognosis. With the deepening of tumor research, the tumor microenvironment has received increasing attention, which is inseparable from the oc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693
Inventor 顾玲李圆圆
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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