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Lymph node-derived lymphoma-related fibroblast tumor cell strain and application thereof

A fibroblast and lymphoma cell technology, applied in the field of biomedicine, can solve the problems of fibroblasts that have not been studied and reported

Pending Publication Date: 2022-07-05
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still no studies and reports on lymphoma, especially cancer-associated fibroblasts derived from lymph nodes.

Method used

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  • Lymph node-derived lymphoma-related fibroblast tumor cell strain and application thereof
  • Lymph node-derived lymphoma-related fibroblast tumor cell strain and application thereof
  • Lymph node-derived lymphoma-related fibroblast tumor cell strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] Example: Isolation and establishment of HXLyAF-KLN

[0028] The HXLyAF-KLN described in this example is derived from the lymph node microenvironment in the human lymphoma cell transplanted tumor model in nude mice, and its cell name is mouse lymphoma-associated fibroblast tumor cell HXLyAF-KLN, and its construction method is as follows:

[0029] (1) The human anaplastic large cell lymphoma cells Karpas299 in logarithmic growth phase were divided into 8 × 10 6 Each mouse / 100µl / mouse was inoculated into male Balb / c (nu / nu) mice (8 weeks old, body weight 22-27g) subcutaneously in the nude area; the tumor formation at the inoculation site was observed every other day, and the mice did not form subcutaneous tumors. Weeks later, human anaplastic large cell lymphoma cells Karpas299 were pressed again by 3 × 10 6 Each / 200µl / mouse was inoculated subcutaneously in the nude area opposite to the previous inoculation site of mice. More than 30 days after the second inoculation, th...

Embodiment 2

[0035] Example 2: Growth and Biological Characterization of HXLyAF-KLN

[0036] (1) Cell morphology observation:

[0037] Take HXLyAF-KLN cells in logarithmic growth phase and observe the morphology of living cells under an inverted microscope, such as figure 1 As shown, the cells grow adherently, mainly irregular polygonal and spindle-shaped, with large nuclei, clear nucleoli, stubby protrusions in the cytoplasm, no contact inhibition, and can cross and overlap growth, and aggregate in clusters when cells are dense; 3 % glutaraldehyde was pre-fixed, 1% osmium tetroxide was re-fixed, uranyl acetate and lead citrate were stained with JEM-1400FLASH fluoroscopy electron microscope to observe the ultrastructure of cells, such as figure 2 It can be seen that the nucleus is irregular in shape, with notch, clear nuclear membrane, obvious nucleolus, increased cytoplasmic rough endoplasmic reticulum, abundant free ribosomes, endoplasmic reticulum, mitochondria and other organelles. ...

Embodiment 3

[0045] Example 3: Construction of mouse fibroblast tumor animal model

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Abstract

The invention discloses a lymph node-derived lymphoma-related fibroblast tumor cell strain which is derived from a lymph node microenvironment in a human lymphoma cell nude mouse transplantation tumor model, the cell name of the cell strain is mouse lymphoma-related fibroblast tumor cells HXLyAF-KLN, the preservation number is CCTCC NO: C2021107, the preservation date is December 9, 2021, and the preservation number is CCTCC NO: C2021107. The preservation unit is China Center for Type Culture Collection. The cell strain is a lymph node-derived lymphoma-related mouse fibroblast tumor cell strain established for the first time at home and abroad, and can be applied to construction of a mouse fibroblast tumor animal model, a malignant lymph node microenvironment model, a 3D tumor culture system and organoid system model, and a lymph node microenvironment and lymphoma progress and drug resistance relationship model.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a lymph node-derived lymphoma-related fibroblast tumor cell line and its application. Background technique [0002] Lymphoma is a group of malignant tumors originating from the lymphatic hematopoietic system. It is one of the common tumors. The incidence has gradually increased in recent years. With fever, night sweats, weight loss, itching and other systemic symptoms. According to the tumor cells, it is divided into two types: Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL). [0003] The occurrence, growth and metastasis of tumors are closely related to the internal and external environment of tumor cells, which is called tumor microenvironment. related to the internal environment. The tumor microenvironment interacts with tumor cells and affects the occurrence, development and prognosis of tumors. [0004] Cancer-associated fibroblasts (CAFs) are the main cellular ...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12N5/071A01K67/027
CPCC12N5/0693C12N5/0656A01K67/0271A01K2207/12A01K2227/105A01K2267/0331Y02A50/30
Inventor 顾玲徐郡李圆圆刘瀚旻
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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