A non-toxic treatment and application method of blood-sucking leech bait

Abstract

The present invention discloses a non-toxic treatment method for blood-sucking leech bait, comprising the steps of ultrasonic treatment of blood, dilution, pH adjustment, temperature increase, rapid detoxification of small molecular hydroxyl groups and oxygen atoms and ozone-rich water without residue, decomposition of ozone and other strong oxidizing substances by reducing agent, etc. The present invention also provides an application method of the blood-sucking leech bait. The treatment method of the present invention not only ensures that the bait nutrition is not lost and the biological safety is prevented, and the infection epidemic or metabolic imbalance death of leeches after feeding is avoided, but also ensures the quality and safety of leeches as important Chinese medicinal materials. Rapid non-toxic treatment is carried out by screening and adding reducing agents, so as to achieve rapid decomposition and reduction of ozone and other strong oxidizing substances. There are no chemical disinfectants and antibiotic drugs in the whole treatment process, no toxic side effects, no residue, which conforms to the green and healthy breeding concept that the country currently vigorously advocates and encourages to develop, is conducive to improving the overall quality of the medical blood-sucking leech breeding market, and has a strong prospect for promotion and application.

Description

Technical Field

[0001] The invention relates to the technical field of blood-sucking leech breeding, and in particular to a non-toxic treatment and application method of blood-sucking leech bait. Background Art

[0002] Leeches, commonly known as leeches, were first recorded in Shennong's Herbal Classic as a low-grade product: "Leech tastes salty and flat, and is used to transport bad blood, treat blood stasis, amenorrhea, break up blood clots, and treat infertility, and promote urination." Leeches, as a traditional Chinese special medicinal aquatic animal, have a history of more than 2,000 years of use. They have the effects of treating stroke, hypertension, clearing blood stasis, amenorrhea, and traumatic injuries. Hirudin is an important biologically active substance in leeches and their salivary glands. Hirudin has a strong inhibitory effect on thrombin and is the strongest natural specific inhibitor of thrombin discovered so far. It has a strong inhibitory effect on coagulation and anti-thrombotic effects.

[0003] The Japanese medicinal leeches and Philippine cattle leeches are fed with fresh animal blood, supplemented with nutritional elements to meet the nutritional needs of leech growth and development, and retain the natural feeding habits of leeches to ensure that their effective ingredients fully meet clinical requirements. The animal blood used for feeding comes from regular slaughterhouses, and the nutritional element supplements used are all food standards to ensure that the feed source is safe and pollution-free.

[0004] In order to obtain non-coagulated plasma components for preparing leech bait, the current method is to add blood anticoagulants containing sodium salts to the plasma components. However, sodium ions will destroy the normal ion metabolic balance, which will cause metabolic disorders in leeches after ingestion, leading to mass deaths.

[0005] Small molecular hydroxyl and oxygen atoms and ozone-rich water refer to small molecular hydroxyl radicals (hydrated hydroxyl ions H3O2 - ), a mixture solution of ozone molecules and oxygen atoms, in which ozone, also known as active oxygen, is easily decomposed into oxygen. The above components all have extremely strong oxidizing ability, and their redox potential is much higher than that of chlorine. Small molecular hydroxyl and oxygen atoms and ozone-rich water can quickly decompose organic and inorganic matter in water, kill bacteria, viruses, parasites and other pathogens in water, and have the characteristics of fast action and no secondary pollution, and can be widely used in food processing and aquaculture.

[0006] High-pressure ozone blood purification is to dissolve high-pressure ozone in human blood in extracorporeal circulation, inactivate various pathogenic microorganisms such as viruses, bacteria, and mycoplasma, and then return it to the human body. The ozone dissolved in the blood is treated with activated carbon adsorption before being returned to avoid the possible adverse effects of residual ozone on the human body. However, the attenuation time of ozone is relatively long, and it usually takes several hours or even dozens of hours for the ozone in the blood to basically decay.

[0007] In this method, the fresh water is treated with small molecular hydroxyl and oxygen atoms and ozone-rich water to produce small molecular hydroxyl radicals (hydrated hydroxyl ions H3O2 - ), strong oxidizing factors such as ozone molecules and oxygen atoms have a certain solidification rate and decay time in fresh water. They are completely degraded and reduced under natural light for 3 hours, and the ORP value drops from 850mV to 260-300mV.

[0008] It can be seen that blood contains strong oxidizing substances such as ozone, which have a greater impact on organisms, and ingesting blood containing strong oxidizing components such as ozone may also cause damage to the organisms that eat it. Leeches use blood as food. If strong oxidizing substances such as ozone are used to pre-treat leech blood bait, it is necessary to comprehensively consider issues such as anticoagulant sodium salt, ozone residues, and blood freshness. Summary of the invention

[0009] The purpose of the present invention is to overcome the defects of the prior art and provide a non-toxic treatment method for blood-sucking leech bait.

[0010] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0011] The present invention provides a non-toxic treatment method for blood-sucking leech bait, comprising the following steps:

[0012] (1) Ultrasonic treatment: fresh blood or frozen blood clots thawed at room temperature are placed in an ultrasonic generator for ultrasonic treatment;

[0013] (2) Dilution: dilute the blood subjected to ultrasound treatment in step (1) with deionized water at a temperature of 36-37° C. to a blood concentration of 30-95%, add a pH adjuster to adjust the pH value to 6.0-6.5 under stirring to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36-37° C.;

[0014] (3) Rapid detoxification without residue: dilute the high concentration of small molecular hydroxyl and oxygen atoms and ozone-rich water with clean purified water or fresh water at a volume ratio of 100-200:1; then add it to the bait solution of step (2) to make the concentration of strong oxidizing substances in the mixed material reach at least 3 mg / L; stir for 3-8 minutes, and then use a redox meter to measure the redox potential ORP of 600-800mV to complete the rapid detoxification treatment;

[0015] (4) Vitamin C reduction: Add food-grade vitamin C reagent to the mixture of step (3) in an amount of 1.0-1.5 ppm, continue stirring for 5-12 minutes, and then measure its redox potential ORP to be 260-320 mV, completing the removal of ozone and other strong oxidizing substances to obtain detoxified leech blood bait.

[0016] Preferably, in step (1), the ultrasonic treatment is repeated three times using an ultrasonic generator, each time for 10 minutes. After each treatment, the mixture is taken out and stirred well before continuing. The mode is set as follows: 90% power, ultrasonic for 1 minute, and stop for 1 minute.

[0017] Preferably, the pH adjuster in step (2) is a citric acid solution with a concentration of 0.05-0.1 mol / L.

[0018] Preferably, the stirring speed for controlling the stirring condition in step (2) is 100-500 rpm, and the stirring speed for controlling the stirring condition in steps (3) and (4) is 200-300 rpm.

[0019] Preferably, the strong oxidizing substances include ozone, oxygen atoms and small molecule hydroxyl radicals.

[0020] The present invention also provides an application method of the detoxified blood-sucking leech bait, which is made into blood sausage with a length of 8-15 cm, and the blood sausage is fed to blood-sucking leeches in different growth stages once every 3-15 days according to different seasons.

[0021] Preferably, when the blood sausage is used to feed blood-sucking leeches, in summer, the feeding frequency of young leeches is 3-5 days / time, and the feeding frequency of commercial leeches is 5-7 days / time; in summer and autumn, the feeding frequency of commercial leeches is 7-10 days / time; during early spring and autumn, the feeding frequency of seed leeches is 10-15 days / time.

[0022] Preferably, when the blood sausage is used to feed young leeches, the blood concentration of the blood-sucking leech bait contained in the blood sausage after dilution in step (2) is controlled to be 80-95%.

[0023] Preferably, when the blood sausage is used to feed commercial leeches or commercial leeches, the blood concentration of the blood-sucking leech bait contained in the blood sausage after dilution in step (2) is controlled to be 60-80%.

[0024] Preferably, when the blood sausage is used to feed leeches, the blood concentration of the blood-sucking leech bait contained in the blood sausage after dilution in step (2) is controlled to be 30-60%.

[0025] The present invention has the following beneficial effects due to the adoption of the above technical solution:

[0026] 1. The present invention uses ultrasonic wave to treat fresh blood or thawed frozen blood clots to obtain a blood homogenate stock solution with uniform texture. The present invention does not add blood anticoagulants such as sodium citrate, thus avoiding the phenomenon of metabolic disorder and mass death of leeches after ingestion due to the introduction of sodium salt.

[0027] 2. In order to preserve the freshness of the blood bait, the present invention uses small molecular hydroxyl and oxygen atoms and ozone-rich water to detoxify it. The water contains small molecular hydroxyl free radicals (hydrated hydroxyl ions H3O2 - ), ozone molecules and oxygen atoms and other strong oxidizing substances, is a stable strong oxidizing source. Hydroxyl oxidation source (-OH), its redox potential is as high as (+2.4V), much higher than common strong oxidants such as ozone (+2.17V), chlorine (+1.77V). The present invention uses high-concentration small molecule hydroxyl and oxygen atoms and ozone-rich water to quickly disinfect and sterilize blood. Due to the rupture of plasma cell membrane, small molecule hydroxyl, oxygen atoms and ozone and other strong oxidizing substances can fully contact with it, and sterilization without dead ends is carried out. Blood bait is fully mixed with small molecule hydroxyl and oxygen atoms and ozone-rich water under stirring. The strong oxidizing substances in the water have strong intercellular penetration and can instantly kill any pathogens carried by blood bait. It acts quickly and has no secondary pollution. When blood bait is needed, it is disinfected, blood sausage is made, and it is fed, so that the blood sausage is kept in a very fresh state, and the freshness and biosafety of the bait are improved to a new level.

[0028] 3. The present invention aims at the characteristics of the breeding environment of leeches. After the blood bait is rapidly detoxified without residue, vitamin C, a reducing agent suitable for freshwater conditions, is selected to quickly consume the remaining strong oxidizing substances such as ozone in the bait. Due to the strong oxidation sterilization and rapid reduction process, the detoxification treatment time is short, and no chemical disinfection drug preparations are used, which minimizes the damage to the taste and nutrition of the blood bait. The blood bait has good palatability and rich nutrition when used as leech bait, promotes its feeding and digestion, is beneficial to the growth of leeches, and improves and guarantees the survival rate and medicinal quality of leeches at different growth stages.

[0029] 4. The present invention also provides an application method of the blood bait. According to the requirements of the digestion and absorption characteristics of leeches of different specifications or growth cycle stages on the concentration of bait, blood baits of different concentrations are made into blood sausages, and the blood sausages are fed at different feeding frequencies according to the season. When the leeches are full and loosen the front sucker jaws, the small holes pierced in the outer wall of the casing will be partially closed. Therefore, feeding in this way is in line with the biological habits of leeches, and the blood of the bait leaked out during the feeding process of leeches will be very little, which not only improves the utilization rate of the bait, but also improves the hygiene of the breeding environment, and can maintain a good breeding growth environment.

[0030] In summary, the blood bait processing method of the blood-sucking leech of the present invention not only ensures that the nutrients of the bait are not lost, but also ensures its biological safety, avoids the infection epidemic or death caused by metabolic imbalance caused by poison and pollution of the bait to the leech, and also ensures the quality and safety of the leech as an important Chinese medicinal material. By screening and adding a high-efficiency reducing agent for rapid non-toxic reduction treatment, the pH value of the bait blood can be adjusted, and the decomposition of strong oxidizing substances such as ozone can be promoted. There are no chemical disinfectants and antibiotics in the entire processing process, and physical and biological organics are used throughout the process. There are no drugs and preparations with toxic and side effects. Residue, which is in line with the green and healthy breeding concept that the country is currently vigorously advocating and encouraging development, is conducive to improving the overall quality of the medical blood-sucking leech breeding market, forming a benign competitive market, and has broad prospects for promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The blood sausage obtained by the method of the invention is used to feed young Philippine cattle leeches.

[0032] Figure 2 The invention relates to commercial adult leeches of Philippine cattle fed with blood sausage obtained by the method of the invention.

[0033] Figure 3 The blood sausage obtained by the method of the present invention is fed to Japanese medical leeches.

[0034] Figure 4 The blood sausage obtained by the method of the present invention is fed to Japanese medical leeches.

[0035] Figure 5 It is a Japanese medicinal leech that is cultivated at a high density. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, preferred embodiments are given below to further describe the present invention in detail. However, it should be noted that many details listed in the specification are only for the purpose of enabling the reader to have a thorough understanding of one or more aspects of the present invention, and these aspects of the present invention can be implemented even without these specific details.

[0037] Example 1

[0038] A bait for blood-sucking leeches, wherein the blood bait after detoxification is made into 8 cm long blood sausage for feeding. In summer, the blood sausage is fed once every 3 days. The detoxification method of the blood bait is as follows:

[0039] (1) Ultrasonic treatment: Purchase fresh blood that has passed the quarantine inspection at a regular livestock slaughterhouse and has no zoonotic pathogens detected. Place the blood in an ultrasonic generator for ultrasonic treatment: each time lasts for 10 minutes. After each time, take out the blood and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0040] (2) Dilution: dilute the blood treated with ultrasound in step (1) with deionized water at a temperature of 36° C. to a blood concentration of 80%, add a 0.05 mol / L citric acid solution while stirring at a speed of 100 rpm to adjust the pH value to 6.0, to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36° C.;

[0041] (3) Rapid detoxification without residue: dilute the high-concentration small molecule hydroxyl and oxygen atoms and ozone-rich water with deionized water at room temperature in a volume ratio of 200:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances in the mixed material 3 mg / L; stir at 300 rpm for 3 minutes, and then use a redox meter to measure the redox potential ORP of 702 mV to complete the rapid detoxification treatment;

[0042] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.0 ppm to the mixture of step (3), continue stirring for 5 minutes, and then measure its redox potential ORP to be 315 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0043] Example 2

[0044] A bait for blood-sucking leeches, wherein the blood bait is detoxified and made into a 9-cm long blood sausage for feeding. In summer, the blood sausage is fed once every 4 days. The detoxification method of the blood bait is as follows:

[0045] (1) Ultrasonic treatment: Purchase fresh blood that has passed the quarantine inspection at a regular livestock slaughterhouse and has no zoonotic pathogens detected. Place the blood in an ultrasonic generator for ultrasonic treatment: each time lasts for 10 minutes. After each time, take out the blood and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0046] (2) Dilution: dilute the blood subjected to ultrasonic treatment in step (1) with deionized water at a temperature of 36.5° C. to a blood concentration of 90%, add a 0.06 mol / L citric acid solution at a concentration of 6.1 while stirring at a speed of 150 rpm to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36.5° C.;

[0047] (3) Rapid detoxification without residue: dilute the high-concentration small molecular hydroxyl and oxygen atoms and ozone-rich water with clean fresh water at room temperature in a volume ratio of 180:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances in the mixed material 3.2 mg / L; stir at 200 rpm for 5 minutes, and then use a redox meter to measure the redox potential ORP of 605 mV to complete the rapid detoxification treatment;

[0048] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.1 ppm to the mixture of step (3), continue stirring for 7 minutes, and then measure its redox potential ORP to be 262 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0049] Example 3

[0050] A bait for blood-sucking leeches, wherein the blood bait after detoxification is made into a 10 cm long blood sausage for feeding. In summer, the blood sausage is fed once every 5 days. The detoxification method of the blood bait is as follows:

[0051] (1) Ultrasonic treatment: Take a frozen blood clot frozen at -20°C from a regular livestock slaughterhouse that has passed quarantine. The blood used does not carry zoonotic pathogens. After the frozen blood clot is naturally thawed at room temperature, it is placed in an ultrasonic generator for ultrasonic treatment: each time is 10 minutes. After each time, take it out and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0052] (2) Dilution: dilute the blood treated with ultrasound in step (1) with deionized water at a temperature of 37° C. to a blood concentration of 95%, add a 0.07 mol / L citric acid solution while stirring at a speed of 200 rpm to adjust the pH value to 6.2 to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 37° C.;

[0053] (3) Rapid detoxification without residue: dilute the high concentration of small molecular hydroxyl and oxygen atoms and ozone-rich water with double distilled water at room temperature in a volume ratio of 170:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 3.5 mg / L; stir at a speed of 215 rpm for 6 minutes, and then use a redox meter to measure the redox potential ORP of 782 mV to complete the rapid detoxification treatment;

[0054] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.2 ppm to the mixture of step (3), continue stirring for 9 minutes, and then measure its redox potential ORP to be 307 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0055] Example 4

[0056] A leech bait in a blood-sucking leech product is made into a blood sausage 11 cm long from the detoxified blood bait for feeding. In summer, the blood sausage is fed once every 6 days. The detoxification method of the blood bait is as follows:

[0057] (1) Ultrasonic treatment: Take a frozen blood clot frozen at -20°C from a regular livestock slaughterhouse that has passed quarantine. The blood used does not carry zoonotic pathogens. After the frozen blood clot is naturally thawed at room temperature, it is placed in an ultrasonic generator for ultrasonic treatment: each time is 10 minutes. After each time, take it out and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0058] (2) Dilution: dilute the blood subjected to ultrasonic treatment in step (1) with deionized water at a temperature of 37° C. to a blood concentration of 60%, add a 0.08 mol / L citric acid solution while stirring at a speed of 250 rpm to adjust the pH value to 6.3, to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36.5° C.;

[0059] (3) Rapid detoxification without residue: dilute the high-concentration small molecule hydroxyl and oxygen atoms and ozone-rich water with pure water at room temperature in a volume ratio of 160:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 3.6 mg / L; stir at 235 rpm for 6 minutes, and then use a redox meter to measure the redox potential ORP of 755 mV to complete the rapid detoxification treatment;

[0060] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.3 ppm to the mixture of step (3), continue stirring for 10 minutes, and then measure its redox potential ORP to be 271 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0061] Example 5

[0062] A commercial leech bait for blood-sucking leeches, wherein the blood bait after detoxification is made into a 12 cm long blood sausage for feeding. In summer and autumn, the blood sausage is fed once every 7 days. The detoxification method of the blood bait is as follows:

[0063] (1) Ultrasonic treatment: Take a frozen blood clot frozen at -20°C from a regular livestock slaughterhouse that has passed quarantine. The blood used does not carry zoonotic pathogens. After the frozen blood clot is naturally thawed at room temperature, it is placed in an ultrasonic generator for ultrasonic treatment: each time is 10 minutes. After each time, take it out and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0064] (2) Dilution: dilute the blood treated with ultrasound in step (1) with deionized water at a temperature of 36° C. to a blood concentration of 70%, add a 0.09 mol / L citric acid solution while stirring at a speed of 300 rpm to adjust the pH value to 6.3, to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 37° C.;

[0065] (3) Rapid detoxification without residue: dilute the high-concentration small molecule hydroxyl and oxygen atoms and ozone-rich water with distilled water at room temperature in a volume ratio of 150:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 3.9 mg / L; stir at 260 rpm for 7 minutes, and then use a redox meter to measure the redox potential ORP of 632 mV to complete the rapid detoxification treatment;

[0066] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.4 ppm to the mixed material in step (3), continue stirring for 10 minutes, and then measure its redox potential ORP to be 288 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0067] Example 6

[0068] A commercial leech bait for blood-sucking leeches is prepared by making blood bait that has been detoxified into a 13 cm long blood sausage for feeding. In summer and autumn, the blood sausage is fed once every 10 days. The detoxification method of the blood bait is as follows:

[0069] (1) Ultrasonic treatment: Take a frozen blood clot frozen at -20°C from a regular livestock slaughterhouse that has passed quarantine. The blood used does not carry zoonotic pathogens. After the frozen blood clot is naturally thawed at room temperature, it is placed in an ultrasonic generator for ultrasonic treatment: each time is 10 minutes. After each time, take it out and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0070] (2) Dilution: dilute the blood ultrasonically treated in step (1) with deionized water at a temperature of 36.8° C. to a blood concentration of 80%, add a 0.1 mol / L citric acid solution at a concentration of 6.4 while stirring at a speed of 350 rpm to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36° C.;

[0071] (3) Rapid detoxification without residue: dilute the high-concentration small molecular hydroxyl and oxygen atoms and ozone-rich water with distilled water at room temperature in a volume ratio of 140:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 4.3 mg / L; stir at 270 rpm for 8 minutes, and then use a redox meter to measure the redox potential ORP of 753 mV to complete the rapid detoxification treatment;

[0072] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.5 ppm to the mixture of step (3), continue stirring for 9 minutes, and then measure its redox potential ORP to be 308 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0073] Example 7

[0074] A leech bait for blood-sucking leeches, wherein the blood bait after detoxification is made into a 14 cm long blood sausage for feeding. In early spring and autumn, the blood sausage is fed once every 10 days. The detoxification method of the blood bait is as follows:

[0075] (1) Ultrasonic treatment: Purchase fresh blood that has passed the quarantine inspection at a regular livestock slaughterhouse and has no zoonotic pathogens detected. Place the blood in an ultrasonic generator for ultrasonic treatment: each time lasts for 10 minutes. After each time, take out the blood and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0076] (2) Dilution: dilute the blood treated with ultrasound in step (1) with deionized water at a temperature of 36° C. to a blood concentration of 30%, add a 0.05 mol / L citric acid solution while stirring at a speed of 400 rpm to adjust the pH value to 6.5, to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36° C.;

[0077] (3) Rapid detoxification without residue: dilute the high-concentration small molecular hydroxyl and oxygen atoms and ozone-rich water with pure water at room temperature in a volume ratio of 120:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 4.5 mg / L; stir at 280 rpm for 8 minutes, and then use a redox meter to measure the redox potential ORP of 684 mV to complete the rapid detoxification treatment;

[0078] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.5 ppm to the mixture of step (3), continue stirring for 11 minutes, and then measure its redox potential ORP to be 311 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0079] Example 8

[0080] A leech bait for blood-sucking leeches, wherein the blood bait after detoxification is made into a 15 cm long blood sausage for feeding. In early spring and autumn, the blood sausage is fed once every 12 days. The detoxification method of the blood bait is as follows:

[0081] (1) Ultrasonic treatment: Purchase fresh blood that has passed the quarantine inspection at a regular livestock slaughterhouse and has no zoonotic pathogens detected. Place the blood in an ultrasonic generator for ultrasonic treatment: each time lasts for 10 minutes. After each time, take out the blood and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0082] (2) Dilution: dilute the blood subjected to ultrasonic treatment in step (1) with deionized water at a temperature of 36.5° C. to a blood concentration of 45%, add a 0.06 mol / L citric acid solution while stirring at a speed of 450 rpm to adjust the pH value to 6.2, to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 37° C.;

[0083] (3) Rapid detoxification without residue: dilute the high-concentration small molecular hydroxyl and oxygen atoms and ozone-rich water with deionized water at room temperature in a volume ratio of 110:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 4 mg / L; stir at 290 rpm for 6 minutes, and then use a redox meter to measure the redox potential ORP of 753 mV to complete the rapid detoxification treatment;

[0084] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.4 ppm to the mixture of step (3), continue stirring for 11 minutes, and then measure its redox potential ORP to be 298 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0085] Example 9

[0086] A leech bait for blood-sucking leeches, wherein the blood bait after detoxification is made into a 15 cm long blood sausage for feeding. In early spring and autumn, the blood sausage is fed once every 15 days. The detoxification method of the blood bait is as follows:

[0087] (1) Ultrasonic treatment: Purchase fresh blood that has passed the quarantine inspection at a regular livestock slaughterhouse and has no zoonotic pathogens detected. Place the blood in an ultrasonic generator for ultrasonic treatment: each time lasts for 10 minutes. After each time, take out the blood and stir it well before continuing. The mode setting is: 90% power, ultrasonic for 1 minute, and stop for 1 minute;

[0088] (2) Dilution: dilute the blood subjected to ultrasonic treatment in step (1) with deionized water at a temperature of 36° C. to a blood concentration of 60%, add a 0.07 mol / L citric acid solution while stirring at a speed of 500 rpm to adjust the pH value to 6.0, to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 37° C.;

[0089] (3) Rapid detoxification without residue: dilute the high-concentration small molecule hydroxyl and oxygen atoms and ozone-rich water with distilled water at room temperature in a volume ratio of 100:1, and then add the bait solution of step (2) to make the concentration of strong oxidizing substances such as ozone in the mixed material 4.5 mg / L; stir at 235 rpm for 6 minutes, and then use a redox meter to measure the redox potential ORP of 746 mV to complete the rapid detoxification treatment;

[0090] (4) Vitamin C reduction: Add food-grade high-purity vitamin C reagent in an amount of 1.2 ppm to the mixture of step (3), continue stirring for 12 minutes, and then measure its redox potential ORP to be 270 mV, completing the removal of strong oxidizing substances to obtain detoxified blood bait for leeches.

[0091] The strong oxidizing substances described in all the above embodiments include ozone, oxygen atoms and small molecular hydroxyl radicals.

[0092] Purification effect comparison

[0093] In order to verify the detoxification effect of the method of the present invention on leech bait, the researchers of the present invention measured the total bacterial content, pH value, DO value, ORP value, ozone content and other indicators of the raw materials in the examples at different stages. The results are shown in Table 1.

[0094] Table 1 Detection indexes of baits during detoxification treatment

[0095]

[0096] As shown in Table 1, before the introduction of small molecule hydroxyl group, oxygen atom and ozone-rich water, the blood bait of Example 1 contained more bacteria and the pH was weakly alkaline. After the introduction of small molecule hydroxyl group, oxygen atom and ozone-rich water for disinfection, the bacteria in the bait were completely killed, the pH increased, and ozone decomposition increased the DO value and ORP value. However, the ozone concentration in the bait was also high, and it was not possible to directly make blood sausage and feed it to blood-sucking leeches. Then vitamin C reagent was added. The vitamin C contained in the reagent showed reducibility when facing strong oxidants, and could quickly consume strong oxidizing substances such as ozone in the solution, so that it was completely decomposed in a relatively short time, and was used for feeding leeches without any adverse effects. In addition, the pH was adjusted to a suitable weakly acidic range for leech feeding. The blood stock solution treated in this way was made into blood sausage and fed to leeches, which not only had no residue and toxic side effects on leeches, but also had the effects of anti-stress, regulating pH, improving body immunity, and promoting digestion, absorption and growth.

[0097] The researchers of the present invention also carried out the above tests on the blood baits of other embodiments, and the test results were consistent with the rules in Table 1. The treated blood baits no longer carried bacteria, and no strong oxidizing factors such as ozone were detected, and the detoxification effect was significant.

Claims

1. A non-toxic treatment method for leech bait, characterized in that: The steps include: (1) Ultrasonic treatment: fresh blood or frozen blood clots thawed at room temperature are placed in an ultrasonic generator for ultrasonic treatment; (2) Dilution: dilute the blood subjected to ultrasound treatment in step (1) with deionized water at a temperature of 36-37° C. to a blood concentration of 30-95%, add a pH adjuster to adjust the pH value to 6.0-6.5 under stirring to obtain a bait solution, and heat the bait solution in a water bath to a temperature of 36-37° C.; (3) Rapid detoxification without residue: dilute high-concentration small molecular hydroxyl and oxygen atoms and ozone-rich water with clean purified water or fresh water in a volume ratio of 100-200:1; small molecular hydroxyl and oxygen atoms and ozone-rich water are used to decompose organic and inorganic matter in water and kill pathogens in water; then add them to the bait solution of step (2) to make the concentration of strong oxidizing substances in the mixed material reach at least 3 mg / L; stir for 3-8 minutes, and then use a redox meter to measure the redox potential ORP of 600-800mV to complete the rapid detoxification treatment; (4) Vitamin C reduction: Add vitamin C reagent to the mixture of step (3) in an amount of 1.0-1.5 ppm, continue stirring for 5-12 minutes, and then measure the redox potential (ORP) to be 260-320 mV, thereby completing the removal of strong oxidizing substances and obtaining a detoxified leech bait; The pH regulator in step (2) is a citric acid solution with a concentration of 0.05-0.1 mol / L; The strong oxidizing substances include ozone, oxygen atoms and small molecular hydroxyl radicals; In step (1), the ultrasonic generator is used to repeat the ultrasonic treatment for 3 times, each time for 10 minutes. After each treatment, the mixture is taken out and stirred well before continuing. The mode is set as follows: 90% power, ultrasonic for 1 minute, and stop for 1 minute.

2. The non-toxic treatment method for blood-sucking leech bait according to claim 1, characterized in that: The stirring speed for controlling the stirring condition in step (2) is 100-500 rpm, and the stirring speed for controlling the stirring condition in steps (3) and (4) is 200-300 rpm.

3. A method for applying a blood-sucking leech bait, characterized in that: The detoxified blood-sucking leech bait according to any one of claims 1 to 2 is stuffed into sausage casings to make blood sausages of 8 to 15 cm in length, and the blood-sucking leeches of different growth stages are fed once every 3 to 15 days according to different seasons.

4. The application method of the blood-sucking leech bait according to claim 3, characterized in that: When the blood sausage is used to feed blood-sucking leeches, in summer, the feeding frequency of young leeches is 3-5 days / time, and the feeding frequency of commercial leeches is 5-7 days / time; in summer and autumn, the feeding frequency of commercial leeches is 7-10 days / time; during early spring and autumn, the feeding frequency of seed leeches is 10-15 days / time.

5. The application method of the blood-sucking leech bait according to claim 3, characterized in that: When the blood sausage is used to feed young leeches, the blood concentration of the blood-sucking leech bait contained in the blood sausage after dilution in step (2) is controlled to be 80-95%.

6. The application method of the blood-sucking leech bait according to claim 3, characterized in that: When the blood sausage is used to feed commercial leeches or commercial leeches, the blood concentration of the blood-sucking leech bait contained in the blood sausage after dilution in step (2) is controlled to be 60-80%.

7. The application method of the blood-sucking leech bait according to claim 3, characterized in that: When the blood sausage is used to feed leeches, the blood concentration of the blood-sucking leech bait contained in the blood sausage after dilution in step (2) is controlled to be 30-60%.

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