An anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein and its application

Abstract

The present invention discloses an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein and its application. According to weight parts, the anti-aging composition includes 0.1-20 weight parts of hydroxypropyl tetrahydropyrantriol, 0.1-20 weight parts of snake venom peptide, 0.1-20 weight parts of recombinant human-like type III collagen, 5-30 weight parts of butanediol and 20-70 weight parts of deionized water. The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein of the present invention can be applied to various skin care formulations such as essence water, essence, emulsion, cream, etc. The anti-aging composition is complementary in the mechanism of action, and the combined application has the effects of increasing skin elasticity, promoting collagen synthesis, promoting the regeneration of damaged tissue, and tightening the skin.

Description

Technical Field

[0001] The invention relates to a skin care product and application thereof, belonging to the technical field of daily chemical products, and in particular to an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein and application thereof in the skin care product. Background Art

[0002] Skin aging can cause changes in the expression level of skin genes, thereby affecting the integrity of the skin's ultrafine structure, leading to dryness, sagging, and wrinkles. The formation of skin wrinkles is related to sagging skin, reduced subcutaneous tissue, bone atrophy, and repeated contraction of facial muscles. They will appear and worsen with age. As our society gradually enters an aging society, anti-aging has received more and more attention. In the 1990s, botulinum toxin began to be used in cosmetic plastic surgery, and its effect in wrinkle removal has also been verified.

[0003] Under the action of botulinum toxin, the facial and neck muscles relax, reducing or eliminating dynamic wrinkles caused by the contraction of the facial muscles, and to some extent delaying the development of wrinkles to achieve a younger look. However, it also has very obvious side effects. Common adverse reactions to botulinum toxin injections include local pain, bruising, red eyes, gastrointestinal symptoms, ptosis, ectropion, strabismus, lip eversion, etc. Botulinum toxin injections are micro-plastic medical cosmetic surgeries, and their effectiveness and safety are limited by medical conditions, and the risks are relatively high.

[0004] Therefore, beauty lovers are in urgent need of a safer, more effective and more convenient anti-aging method. Among them, the use of topical anti-aging cosmetics has become the preferred and simple method. However, although ordinary anti-aging cosmetics are milder and safer than botulinum toxin, the anti-aging active ingredients in them are single and are only added conceptually in some cosmetics. Even if the amount is sufficient, their transdermal absorption and physiological activity are not considered. Therefore, their effects cannot meet consumers' expectations. In terms of anti-aging and wrinkle removal effects, they are far less obvious than botulinum toxin.

[0005] Whether male or female, as they age, there will be some obvious changes in the epidermis and dermis. These changes are mainly reflected in the following aspects: slower metabolic rate of keratinocytes, less intercellular lipids, thinning of the epidermis, weakened defense ability, increased TEWL, pigmentation, uneven skin color, weakened epidermal-dermal connection, protein cross-linking and reduction in the dermis, loss of skin elasticity, and reduced number and activity of fibroblasts.

[0006] It is generally believed that the effects of anti-skin aging active substances include: promoting the synthesis of mucopolysaccharides and collagen, promoting the regeneration of damaged tissues, increasing skin elasticity, and tightening the skin. Summary of the invention

[0007] In order to solve the above problems existing in the prior art, the purpose of the present invention is to provide an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein. The anti-aging composition of the present invention is complementary in action mechanism, and the combined application has the effects of increasing skin elasticity, promoting collagen synthesis, promoting the regeneration of damaged tissue, and tightening the skin.

[0008] Another object of the present invention is to provide a preparation method and application of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, which has a simple process and saves production time. The stability of the active ingredients can be maintained during the preparation process, and the composition can also have obvious anti-aging and anti-wrinkle effects.

[0009] The technical solution adopted by the present invention is:

[0010] An anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, wherein the anti-aging composition is composed of the following raw material components by weight:

[0011] Hydroxypropyl tetrahydropyrantriol 0.1-20 parts by weight;

[0012] Snake venom peptide 0.1-20 parts by weight;

[0013] 0.1-20 parts by weight of recombinant human-like type III collagen;

[0014] Butanediol 5-30 parts by weight;

[0015] Deionized water 20-70 parts by weight.

[0016] Furthermore, the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein is characterized in that, by weight, the anti-aging composition is composed of the following raw material components:

[0017] Hydroxypropyl tetrahydropyrantriol 1-20 parts by weight;

[0018] 1-10 parts by weight of snake venom peptide;

[0019] 2-10 parts by weight of recombinant human type III collagen;

[0020] Butanediol 5-15 parts by weight;

[0021] Deionized water 40-65 parts by weight.

[0022] In addition, the present invention also discloses a method for preparing the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, comprising the following steps:

[0023] 1) dissolving hydroxypropyl tetrahydropyrantriol and butylene glycol in deionized water and stirring evenly to prepare a hydroxypropyl tetrahydropyrantriol solution, i.e., component A;

[0024] 2) Add snake venom peptide, recombinant human-like type III collagen, and component A to deionized water in sequence, and stir evenly to obtain an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein.

[0025] Furthermore, in step 1), the stirring speed is 20-80 r / min, and in step 2), the stirring speed is 30-100 r / min.

[0026] Furthermore, in step 2), the ratio of the total mass of the three raw materials, hydroxypropyl tetrahydropyrantriol, snake venom peptide, and recombinant human-like type III collagen, to the mass of water is 1:0.5-2.5.

[0027] Furthermore, the ratio of the total mass of the three raw materials, hydroxypropyl tetrahydropyrantriol, snake venom peptide and recombinant human-like type III collagen, to the mass of water is 1:1.8.

[0028] The invention discloses an application of an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein in skin care products. The skin care products contain 10-40% of the anti-aging composition and 60-90% of conventional auxiliary materials in the field of skin care products, based on the total weight of the skin care products.

[0029] Furthermore, the conventional auxiliary materials include surfactants, thickeners, conditioners, moisturizers, preservatives, chelating agents, flavors, and deionized water.

[0030] Furthermore, the skin care products are made into various dosage forms such as toner, softener, firmer, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, facial mask, powder, body lotion, body cream, body oil, body milk and body essence.

[0031] Furthermore, the skin care product is made into an anti-aging and wrinkle-lightening essence, and the raw material components of the anti-aging and wrinkle-lightening essence include:

[0032]

[0033] The anti-aging and wrinkle-lightening essence is prepared by the following steps:

[0034] 1) After mixing deionized water, sodium hyaluronate, xanthan gum and allantoin, heat at 85°C for 30 min and stir at 50 r / min for 10 min to obtain a phase A raw material;

[0035] 2) The raw material of phase A is cooled to below 40° C., an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, oat extract and preservative are added, and the mixture is stirred at 70 r / min for 5 min. When the temperature is cooled to below 35° C., the mixture is packaged to obtain the anti-aging and wrinkle-reducing essence.

[0036] The preservative is selected from polyols such as hexylene glycol and phenoxyethanol.

[0037] Compared with the prior art, the beneficial effects of the present invention are:

[0038] 1) The anti-aging composition provided by the present invention simultaneously contains hydroxypropyl tetrahydropyrantriol, snake venom peptide, recombinant human-like type III collagen and other polypeptide proteins, which are complementary in action mechanism, and their combined application has a better anti-aging and wrinkle-removing effect.

[0039] 2) The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein provided by the present invention has a simple preparation method and saves production time. The stability of the active ingredients can be maintained during the preparation process, and the composition can also have obvious anti-aging and anti-wrinkle effects.

[0040] 3) The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein of the present invention is safe and non-irritating, and can be easily added into the formulation system of various dosage forms of cosmetics, and thus has important practical significance and broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 The present invention provides a diagram of the mechanism of action of snake venom peptide in an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein.

[0042] Figure 2 The present invention provides a structural diagram of recombinant human-like type III collagen in an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein. DETAILED DESCRIPTION

[0043] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0044] The invention discloses an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein. The anti-aging composition is prepared by taking hydroxypropyl tetrahydropyrantriol, a polypeptide protein composition (snake venom peptide, recombinant human-like type III collagen) and butanediol as raw materials and combining them in a suitable weight ratio.

[0045] The following are descriptions of the technical effects of the raw material components.

[0046] ① Hydroxypropyl tetrahydropyrantriol

[0047] Hydroxypropyl tetrahydropyrantriol is a xylose derivative derived from Western European beech wood. It is an anti-aging active ingredient first developed by Lancome laboratories. It has the effect of promoting the synthesis of collagen and glycosaminoglycans, targeting the junction between the epidermis and dermis DEJ, helping the dermis maintain elasticity.

[0048] ②Snake venom peptide (dipeptide diaminobutyryl benzylamide diacetate)

[0049] The standard Chinese name of snake venom peptide is dipeptide diaminobutyryl benzylamide diacetate. It is a synthetic peptide that simulates snake venom serum. It can block the nerve conduction of acetylcholine and relax muscles, thereby improving dynamic wrinkles. Figure 1 shown.

[0050] ③Recombinant human type III collagen

[0051] Collagen determines the age of the skin. Recombinant human type III collagen is an innovative collagen derived from biological fermentation technology. It uses human collagen gene as a template, is optimized and transformed, introduced into Pichia yeast, and obtained through fermentation induction, separation and purification. The obtained collagen is highly consistent with human collagen and has good skin affinity and tissue compatibility. Safety, efficacy and human trials have proven that recombinant human type III collagen is a safe and efficient collagen that can be directly used by the skin. The transdermal rate reaches 80%-90%. The transdermal process is safe and non-irritating, fast and long-lasting, and has a strengthening effect on the stratum corneum.

[0052] The structure of recombinant human type III collagen is shown in Figure 2 As shown, its functions are as follows:

[0053] 1. Reconstruct cellular water channels and ultrastructure, maintain cell and blood vessel reconstruction, and inhibit apoptosis of aging cells; 2. Inhibit inflammatory damage; 3. Restore extracellular matrix reconstruction; 4. Improve the skin and mucosal barrier.

[0054] The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein has different action mechanisms among the components, and is supplemented with moisturizers such as butylene glycol to enhance its permeability. The multiple components act synergistically with each other. The finally prepared anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein has the effects of increasing skin elasticity, promoting collagen synthesis, promoting the regeneration of damaged tissues, and tightening the skin.

[0055] In the following examples, the unit of parts by weight may be g or kg.

[0056] All raw materials of the present invention are commercially available. Hydroxypropyl tetrahydropyrantriol was purchased from Shanghai Keqin Technology Co., Ltd., and snake venom peptide was purchased from DSM. Recombinant human-like type III collagen, molecular weight 5000-10000 Daltons, was purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd. Oat extract was purchased from Symrise.

[0057] Example 1

[0058] This embodiment provides an anti-aging and wrinkle-lightening essence. The raw material components of the essence include, by weight:

[0059] Deionized water 57.7 parts by weight;

[0060] 40 parts by weight of an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein;

[0061] Sodium hyaluronate 0.05 parts by weight;

[0062] Xanthan gum 0.05 weight part;

[0063] Allantoin 0.2 parts by weight;

[0064] 1 part by weight of oat extract;

[0065] 1 part by weight of phenoxyethanol;

[0066] The raw material components of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein include:

[0067] 16 parts by weight of hydroxypropyl tetrahydropyrantriol;

[0068] 10 parts by weight of snake venom peptide;

[0069] 8 parts by weight of recombinant human type III collagen;

[0070] Butanediol 15 parts by weight;

[0071] Deionized water 51 parts by weight;

[0072] The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein is prepared by the following method:

[0073] 1) dissolving hydroxypropyl tetrahydropyrantriol and butylene glycol in deionized water, stirring evenly at a stirring speed of 50 r / min, to prepare a hydroxypropyl tetrahydropyrantriol solution, i.e., component A;

[0074] 2) Add snake venom peptide, recombinant human-like type III collagen, and component A to deionized water in sequence, and stir evenly at a stirring speed of 40 r / min to obtain an anti-aging composition of anti-aging hydroxypropyl tetrahydropyrantriol and polypeptide protein.

[0075] The preparation method of the anti-aging and wrinkle-lightening essence described in this embodiment comprises the following steps:

[0076] 1) After mixing deionized water, sodium hyaluronate, xanthan gum and allantoin, heat at 85°C for 30 min and stir at 50 r / min for 10 min to obtain a phase A raw material;

[0077] 2) The raw material of phase A is cooled to below 40° C., an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, oat extract and preservative are added, and the mixture is stirred at 70 r / min for 5 min. When the temperature is cooled to below 35° C., the mixture is packaged to obtain the anti-aging and wrinkle-reducing essence.

[0078] Example 2

[0079] This embodiment provides an anti-aging and wrinkle-lightening essence. The raw material components of the essence include, by weight:

[0080] Deionized water 67.7 parts by weight;

[0081] 30 parts by weight of an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein;

[0082] Sodium hyaluronate 0.05 parts by weight;

[0083] Xanthan gum 0.05 weight part;

[0084] Allantoin 0.2 parts by weight;

[0085] 1 part by weight of oat extract;

[0086] 1 part by weight of hexylene glycol;

[0087] The raw material components of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein include:

[0088] 16 parts by weight of hydroxypropyl tetrahydropyrantriol;

[0089] 10 parts by weight of snake venom peptide;

[0090] 8 parts by weight of recombinant human type III collagen;

[0091] Butanediol 15 parts by weight;

[0092] Deionized water 51 parts by weight;

[0093] The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein is prepared by the following method:

[0094] 1) dissolving hydroxypropyl tetrahydropyrantriol and butylene glycol in deionized water, stirring evenly at a stirring speed of 50 r / min, to prepare a hydroxypropyl tetrahydropyrantriol solution, i.e., component A;

[0095] 2) Add snake venom peptide, recombinant human-like type III collagen, and component A to deionized water in sequence, and stir evenly at a stirring speed of 40 r / min to obtain an anti-aging hydroxypropyl tetrahydropyrantriol and polypeptide protein composition.

[0096] The preparation method of the anti-aging and wrinkle-lightening essence described in this embodiment comprises the following steps:

[0097] 1) After mixing deionized water, sodium hyaluronate, xanthan gum and allantoin, heat at 85°C for 30 min and stir at 50 r / min for 10 min to obtain a phase A raw material;

[0098] 2) The raw material of phase A is cooled to below 40° C., an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, oat extract and preservative are added, and the mixture is stirred at 70 r / min for 5 min. When the temperature is cooled to below 35° C., the mixture is packaged to obtain the anti-aging and wrinkle-reducing essence.

[0099] Example 3

[0100] This embodiment provides an anti-aging and wrinkle-lightening essence. The raw material components of the essence include, by weight:

[0101] Deionized water 87.7 parts by weight;

[0102] 10 parts by weight of an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein;

[0103] Sodium hyaluronate 0.05 parts by weight;

[0104] Xanthan gum 0.05 weight part;

[0105] Allantoin 0.2 parts by weight;

[0106] 1 part by weight of oat extract;

[0107] 1 part by weight of phenoxyethanol;

[0108] The raw material components of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein include:

[0109] 16 parts by weight of hydroxypropyl tetrahydropyrantriol;

[0110] 10 parts by weight of snake venom peptide;

[0111] 8 parts by weight of recombinant human type III collagen;

[0112] Butanediol 15 parts by weight;

[0113] Deionized water 51 parts by weight;

[0114] The anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein is prepared by the following method:

[0115] 1) dissolving hydroxypropyl tetrahydropyrantriol and butylene glycol in deionized water, stirring evenly at a stirring speed of 50 r / min, to prepare a hydroxypropyl tetrahydropyrantriol solution, i.e., component A;

[0116] 2) Add snake venom peptide, recombinant human-like type III collagen, and component A to deionized water in sequence, and stir evenly at a stirring speed of 40 r / min to obtain an anti-aging hydroxypropyl tetrahydropyrantriol and polypeptide protein composition.

[0117] The preparation method of the anti-aging and wrinkle-lightening essence described in this embodiment comprises the following steps:

[0118] 1) After mixing deionized water, sodium hyaluronate, xanthan gum and allantoin, heat at 85°C for 30 min and stir at 50 r / min for 10 min to obtain a phase A raw material;

[0119] 2) The raw material of phase A is cooled to below 40° C., an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, oat extract and preservative are added, and the mixture is stirred at 70 r / min for 5 min. When the temperature is cooled to below 35° C., the mixture is packaged to obtain the anti-aging and wrinkle-reducing essence.

[0120] Blank example 1

[0121] The difference between this blank example 1 and Example 1 is that the raw material components of the anti-aging and wrinkle-lightening essence are different. The anti-aging and wrinkle-lightening essence in this comparative example does not contain hydroxypropyl tetrahydropyrantriol and the anti-aging composition of polypeptide protein. Other operations are the same as Example 1.

[0122] Effect verification test example

[0123] 1. Safety test-skin patch test

[0124] Test subjects: the anti-aging and wrinkle-reducing essences obtained in Examples 1-3 and the anti-aging and wrinkle-reducing essences obtained in Blank Example 1.

[0125] Number of participants: 150 people, half male and half female, aged between 20 and 55 years old, all meeting the criteria for subjects.

[0126] Experimental process: 150 subjects who met the subject criteria were randomly divided into 5 groups, corresponding to the anti-aging and wrinkle-lightening essence obtained in Examples 1-3, the anti-aging and wrinkle-lightening essence obtained in Blank Example 1, and a control group, with 30 subjects in each group.

[0127] The anti-aging and wrinkle-reducing essence of the test object is placed in each small chamber of the spot tester, with a dosage of about 0.020mL-0.025mL, and nothing is placed in the control well.

[0128] The patch tester with the test substance added is applied to the back or curved side of the forearm of the subject with a hypoallergenic tape (manufacturer: Shanghai Medical Device (Group) Co., Ltd. Sanitary Materials Factory, patch test tape product number: 09000251116), and lightly pressed with the palm of the hand to make it evenly applied to the skin for 24 hours; 30 minutes, 24 hours and 48 hours after removing the test substance patch tester, observe the skin reaction according to the standards shown in Table 1, and record the observation results. The grading standards are shown in Table 1, and the test results are shown in Table 2.

[0129] Table 1 Skin closure patch test skin reaction grading standard

[0130]

[0131] Table 2 Human skin patch test results of Examples 1-3 of the present invention and Blank Example 1

[0132]

[0133]

[0134] It can be seen from Table 2 that the anti-aging and wrinkle-reducing essences obtained in Examples 1-3 of the present invention and Blank Example 1 have no irritation to the skin.

[0135] 2. Efficacy Test

[0136] Test object: the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein in Example 1.

[0137] The anti-aging and wrinkle-reducing essence obtained in Examples 1-3 and the anti-aging and wrinkle-reducing essence obtained in Blank Example 1.

[0138] 1) Oxygen free radical inhibition percentage test

[0139] The photon energy of ultraviolet rays is absorbed by the atoms or molecules of cells, causing changes in the electronic energy level state of the atoms and producing a large number of free radicals, which will hinder the normal development of cells, interfere with cell metabolism, and destroy the mitochondria in cells.

[0140] Test method: Using Organotypical skin model The skin model was pretreated with the anti-aging and wrinkle-lightening essence of the present invention, and Ve and Vc were used as the control group. The skin model was irradiated with ultraviolet rays of different intensities (0-30 mJ / cm 2 The UVB intensity was measured and the oxygen free radical inhibition percentage was measured. The results are shown in Table 3.

[0141] Table 3 Test results

[0142]

[0143]

[0144] It can be seen from Table 3 that the anti-aging and wrinkle-reducing essences obtained in Examples 1-3 of the present invention can effectively reduce oxygen free radicals caused by ultraviolet rays, while the anti-aging and wrinkle-reducing essence obtained in Blank Example 1 has a weaker ability to reduce oxygen free radicals caused by ultraviolet rays. This indicates that the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein can effectively reduce oxygen free radicals caused by ultraviolet rays.

[0145] 2) Experiment on promoting proliferation of human keratinocytes (HACAT cells)

[0146] When studying the aging of the body and skin, people have mostly focused on the aging of skin fibroblasts. In recent years, it has been gradually recognized that keratinocytes also play a very important role in skin aging. With age, skin aging is manifested by slower epidermal renewal, weakened barrier function, and reduced keratinocyte activity.

[0147] Test method: An in vitro cytological model of keratinocytes was established. Hacat cells were cultured in a 24-well plate, and 0.1%, 0.3%, and 0.5% of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein in Example 1 were added. Hoechst 3342 nucleic acid dye was added after 24 hours. Incubate for 15 minutes, and then take pictures. The statistical results are shown in Table 4.

[0148] Table 4 Test results

[0149]

[0150] It can be seen from Table 4 that the anti-aging composition containing 0.1%, 0.3% and 0.5% hydroxypropyl tetrahydropyrantriol and polypeptide protein obtained in Example 1 of the present invention can effectively promote the growth of hacat cells.

[0151] 3) Synergistic effect experiment example

[0152] Comparative Examples 1-6

[0153] The different proportions of raw material components of an anti-aging and wrinkle-reducing essence are shown in Table 5 below.

[0154] Table 5 Comparative Examples 1-6 Raw Materials Ratio (Unit: Weight Part)

[0155]

[0156] The preparation process is the same as described in Example 1 above.

[0157] The anti-wrinkle effect test was carried out on Example 1 and Comparative Examples 1-6. The test results after one month are shown in Table 6.

[0158] Table 6 Test results

[0159]

[0160] It can be seen from the test data in Table 6 that the anti-wrinkle effect is ranked as Example 1 > Comparative Example 4 > Comparative Example 5 > Comparative Example 6 > Comparative Example 1 > Comparative Example 2 > Comparative Example 3, which proves that the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein has better anti-aging effect than the combination of one or two ingredients alone through the synergistic effect between the components.

[0161] 3. Human Trial Evaluation Experiment

[0162] Test subjects: the anti-aging and wrinkle-lightening essence obtained in Examples 1-3, the anti-aging and wrinkle-lightening essence obtained in Blank Example 1, and the commercially available anti-aging and wrinkle-lightening essence. The above essences were used for the test: 150 subjects, 30 in each group, numbered 1-30, and the subjects used the essences on their faces twice a day for 1 month.

[0163] Skin elasticity was measured using the German CK skin elasticity tester MPA580 and the Reviscometer RV600 probe. Wrinkles, firmness, fineness and glossiness were measured using a skin detector, specifically the Visia skin detector.

[0164] Table 7 Test results

[0165]

[0166] It can be seen from the test data in Table 7 that the anti-aging and wrinkle-lightening essences obtained in Examples 1-3 of the present invention can effectively improve the skin wrinkle area and lighten wrinkles, while the skin elasticity, firmness and smoothness are also significantly improved, and the effect is better than that of Blank Example 1 and commercially available anti-aging and wrinkle-lightening essences.

[0167] In summary, the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein of the present invention can promote collagen synthesis, promote the regeneration of damaged tissues, increase skin elasticity, enhance skin firmness and fineness, and reduce skin wrinkles.

[0168] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. An anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein, characterized in that: The anti-aging composition is composed of the following raw material components by weight: Hydroxypropyl tetrahydropyrantriol 1-20 parts by weight; 1-10 parts by weight of snake venom peptide; 2-10 parts by weight of recombinant human type III collagen; Butanediol 5-15 parts by weight; Deionized water 40-65 parts by weight; The anti-aging composition is prepared by the following method: 1) dissolving hydroxypropyl tetrahydropyrantriol and butylene glycol in deionized water and stirring evenly to prepare a hydroxypropyl tetrahydropyrantriol solution, i.e., component A; 2) Adding snake venom peptide, recombinant human-like type III collagen, and component A to deionized water in sequence, and stirring evenly to obtain an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein; The ratio of the total mass of the three raw materials, hydroxypropyl tetrahydropyrantriol, snake venom peptide and recombinant human-like type III collagen, to the mass of water is 1:1.

5.

2. The method for preparing the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein according to claim 1, characterized in that: The steps include: 1) dissolving hydroxypropyl tetrahydropyrantriol and butylene glycol in deionized water and stirring evenly to prepare a hydroxypropyl tetrahydropyrantriol solution, i.e., component A; 2) Add snake venom peptide, recombinant human-like type III collagen, and component A to deionized water in sequence, and stir evenly to obtain an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein.

3. The preparation method according to claim 2, characterized in that: In step 1), the stirring speed is 20-80 r / min, and in step 2), the stirring speed is 30-100 r / min.

4. An application of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein according to claim 1 in skin care products, characterized in that: Based on the total weight of the skin care product, the skin care product comprises 10-40% of the anti-aging composition and 60-90% of conventional auxiliary materials in the field of skin care products.

5. The use of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein as claimed in claim 4 in skin care products, characterized in that: The conventional auxiliary materials include surfactants, thickeners, conditioners, preservatives, chelating agents, flavors, and deionized water.

6. The use of the anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein as claimed in claim 5 in skin care products, characterized in that: The skin care product is made into various dosage forms such as nutrient lotion, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, facial mask, powder cake, body lotion, body oil and body milk.

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