A polypeptide separation and purification system

Abstract

The present invention relates to the technical field of polypeptide purification equipment, specifically a polypeptide separation and purification system, including a purification tank, a support frame is installed at the bottom end of the purification tank, a liquid adding tank and a liquid outlet pipe are connected to the outer side walls of the bottom and top of the purification tank respectively, a mounting frame is detachably installed at the middle end of the purification tank, a screen is installed on the inner side wall of the mounting frame, and the screen is located between the liquid adding tank and the liquid outlet pipe, two cylinders are symmetrically installed on the outer side wall of the purification tank, and the output ends of the two cylinders are jointly installed with a sealed bottom cover that cooperates with the purification tank, and the mounting frame is detachably connected to the top of the sealed bottom cover, and the sealed bottom cover and the bottom end of the purification tank are provided with a positioning component. The present invention can timely remind to replace the screen when the screening effect of the screen deteriorates by setting an indication component, so that the screen can be replaced in time at an appropriate time, which not only reduces the labor intensity of manual labor and avoids the waste of materials such as adsorbents, but also further improves the purification quality of the polypeptide.

Description

Technical Field

[0001] The invention relates to the technical field of polypeptide purification equipment, in particular to a polypeptide separation and purification system. Background Art

[0002] Fast protein liquid chromatography is a form of liquid chromatography widely used in academia and industry for separating and / or purifying a large number of various biomolecules (such as proteins and DNA) from complex mixtures. In FPLC, a mixture containing soluble components is separated through a stationary phase with the help of liquid flow, so that a single component in the mixture is separated. Based on the elution method adopted, the components contained in the mixture migrate through the stationary phase at different speeds or are retained by interacting with specific ligands of the stationary phase, thereby separating from each other. The elution speed in the existing polypeptide purification preparation device is slow, the production efficiency is low, and the sieve plate is connected to the cylinder as a whole, which makes it difficult to clean the sieve plate, affecting the purification rate.

[0003] After searching, Chinese patent number CN212882339U discloses a polypeptide purification and preparation device, including a polypeptide purification and preparation device body and a vibration device. By setting a heating tube, the reaction efficiency can be improved. The arrangement of a pressure column and a vacuum device, pressurizing the cylinder through a vacuum pump, setting the air pressure of the pressure column, and increasing it to a suitable air pressure, speeds up the elution speed, and avoids the situation where the elution speed slows down due to the outflow of liquid and the loss of air. By setting a spring and a snap-fit ​​part, the screen is easy to install and disassemble, and it is convenient to take it down to clean the screen and fill the adsorbent, thereby improving the purification efficiency of the polypeptide. By rotating the rotating disk, the cylinder is automatically reversed, thereby achieving the purpose of full contact and adsorption between the sample and the eluent.

[0004] Although the above-mentioned polypeptide purification and preparation device can pressurize the cylinder through a vacuum pump to speed up the elution speed and can install and disassemble the screen, it still has the following shortcomings:

[0005] 1. When the filtration effect of the screen deteriorates, it is impossible to issue a replacement instruction in time. The screen can only be replaced based on experience. If it is replaced too early, the labor intensity of the manual labor will be increased and the adsorbent will be wasted. If it is replaced too late, the purification quality of the peptide will be reduced.

[0006] 2. The screen is inconvenient to install and disassemble, and the stability of the screen is poor after installation, which not only leads to a decrease in the purification quality of the peptide, but also easily causes a lot of noise after the liquid impacts the screen;

[0007] 3. The components filtered by the screen all stay on the top of the screen and fall on the top wall of the screen due to their own weight, which is not only inconvenient to clean, but also seriously affects the screening effect of the screen, thereby reducing the purification quality of the polypeptide.

[0008] Therefore, a polypeptide separation and purification system is proposed. Summary of the invention

[0009] The object of the present invention is to provide a polypeptide separation and purification system, which solves the problems raised in the above-mentioned background technology by setting an indicator component to promptly remind the user to replace the screen when the screening effect of the screen deteriorates.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] A polypeptide separation and purification system comprises a purification tank, wherein a support frame is installed at the bottom end of the purification tank, a liquid adding tank and a liquid outlet pipe are connected to the outer side walls of the bottom end and the top end of the purification tank respectively, a mounting frame is detachably installed at the middle end of the purification tank, a screen is installed on the inner side wall of the mounting frame, and the screen is located between the liquid adding tank and the liquid outlet pipe, two cylinders are symmetrically installed on the outer side wall of the purification tank, a sealed bottom cover cooperating with the purification tank is installed at the output ends of the two cylinders, and the mounting frame is detachably connected to the sealed bottom cover The top of the purification tank is provided with a positioning component, the sealed bottom cover and the bottom of the purification tank are provided with a positioning component, the liquid adding tank is connected with a polypeptide storage tank and an eluent tank, a mixing mechanism is connected between the liquid adding tank and the purification tank, the bottom of the purification tank is connected with a pressurizing component, and the pressurizing component is driven by the mixing mechanism, an indicating component cooperating with the screen is provided in the purification tank, the indicating component is used for timely reminding to replace the screen with poor screening effect, a nozzle is installed on the inner side wall of the top of the purification tank, and the top of the nozzle is connected with a cleaning liquid pipe.

[0012] The liquid adding tank injects the polypeptide solution and the eluent that cooperates with the polypeptide solution into the purification tank through the bottom end of the purification tank. The eluted polypeptide solution is purified by the screen and then discharged through the liquid outlet pipe at the top. The components filtered by the screen all fall to the bottom end of the screen due to their own weight, which is not only convenient for cleaning from the bottom end of the purification tank, but also improves the screening effect of the screen, thereby improving the purification quality of the polypeptide; the mixing mechanism can mix and stir the polypeptide solution and the eluent added to the purification tank, improve the elution effect of the polypeptide solution, thereby further improving the purification quality of the polypeptide; the pressurizing component can continuously pressurize the purification tank, thereby avoiding the situation where the elution speed is slowed down due to the outflow of liquid and the loss of air, thereby improving the elution and purification rate of the polypeptide; the pressurizing component is jointly driven by the mixing mechanism, thereby avoiding the need to set up a separate driving device to drive the pressurizing component, thereby reducing the manufacturing and use costs of the pressurizing component and the polypeptide separation and purification system.

[0013] Preferably, an annular sealing plate is installed on the top wall of the sealing bottom cover, an annular sealing groove that cooperates with the annular sealing plate is opened at the bottom end of the purification tank, a sealing gasket that cooperates with the annular sealing plate is installed on the inner side wall of the annular sealing groove, a plurality of mounting rods are installed at the top end of the sealing bottom cover, an annular mounting seat is commonly installed at the top end of the mounting rods, a fixing frame is installed at the top end of the annular mounting seat, a threaded hole that meshes with the mounting frame is opened on the inner side wall of the fixing frame, and an annular positioning plate that cooperates with the mounting frame is installed on the inner side wall of the bottom end of the fixing frame.

[0014] The starting cylinder can drive the sealed bottom cover to drive the mounting rod, the annular mounting seat and the fixed frame to move up and down. By moving the fixed frame to the bottom end of the purification tank, the mounting frame and the screen can be conveniently threadedly connected to or removed from the fixed frame. By moving the fixed frame into the purification tank and attaching the sealed bottom cover to the bottom end of the purification tank, the mounting frame and the screen can be installed in the purification tank. The screen can be conveniently installed and removed, and the screen has good stability after installation. It can not only avoid loud noise caused by liquid impacting the screen, but also significantly improve the purification quality of the polypeptide. A control valve is arranged in the sealed bottom cover. When the purification tank needs to be drained, the remaining liquid in the purification tank can be discharged by opening the control valve.

[0015] Preferably, the positioning assembly includes two connecting plates symmetrically installed on the top wall of the sealed bottom cover, and two fixing seats are symmetrically installed on the outer side wall of the bottom end of the purification tank, the bottom ends of the two fixing seats are provided with connecting grooves that cooperate with the connecting plates, and limiting blocks are elastically installed on the outer side walls on the opposite sides of the two connecting plates, and limiting holes that cooperate with the limiting blocks are opened on the inner side walls of the two connecting grooves, and push rods that cooperate with the limiting blocks are threadedly connected to the outer side walls of the two fixing seats, and one end of the push rods extends into the corresponding limiting holes.

[0016] When the sealed bottom cover moves upward, the connecting plate moves upward accordingly. When the sealed bottom cover is tightly attached to the bottom end of the purification tank, the two connecting plates are respectively inserted into the connecting grooves at the bottom ends of the two fixing seats. At this time, the two limiting plugs are pushed into the corresponding limiting plug holes by elastic force. Therefore, the limiting plugs, the connecting plate and the sealed bottom cover can be fixedly connected to the bottom end of the purification tank, thereby improving the stability and sealing of the sealed bottom cover installation. Therefore, it can be avoided that the sealed bottom cover becomes loose when pressurized in the purification tank, resulting in poor sealing of the purification tank, thereby improving the stability of the purification tank and the polypeptide separation and purification system processing.

[0017] Preferably, the mixing mechanism includes a mixing tube, and the liquid adding tank is fixed on the top wall of one end of the mixing tube, a spiral feeding rod is rotatably installed in the mixing tube, a liquid inlet pipe is connected between the mixing tube and the purification tank, a one-way liquid inlet valve is installed in the liquid inlet pipe, a driving motor is installed at the end of the mixing tube away from the liquid inlet pipe, and one end of the spiral feeding rod is connected to the output end of the driving motor.

[0018] The liquid adding tank injects the polypeptide solution and the eluent that cooperates with the polypeptide solution into the mixing tube via the polypeptide storage tank and the eluent tank. Starting the driving motor can drive the spiral feeding rod to rotate. The rotation of the spiral feeding rod can fully mix and stir the polypeptide solution and the eluent in the mixing tube and evenly transport them to the bottom of the purification tank through the liquid inlet pipe, thereby improving the elution effect of the polypeptide solution, thereby further improving the purification quality of the polypeptide.

[0019] Preferably, the pressurizing assembly includes a fixing frame fixed to the bottom end of the mixing tube, a bidirectional screw rod is horizontally rotatably mounted on the fixing frame, a slider is threadedly connected to the bidirectional screw rod, a transmission belt is commonly mounted on one end of the bidirectional screw rod adjacent to the spiral feeding rod, a cylinder body is provided at one end of the bidirectional screw rod, a piston is mounted in the cylinder body, two fixing rods are symmetrically mounted at one end of the piston, and one end of each fixing rod is connected to the slider, an air inlet pipe and an air outlet pipe are mounted at one end of the cylinder body, a one-way air valve is mounted on each of the air inlet pipe and the air outlet pipe, and one end of the air outlet pipe is connected to the outer side wall of the bottom end of the purification tank.

[0020] The driving motor drives the spiral feeding rod to rotate, which can drive the transmission belt and the bidirectional lead screw to rotate. The rotation of the bidirectional lead screw can drive the slider and the piston to reciprocate horizontally. When the piston moves to the side away from the purification tank, the gas can be sucked into the cylinder through the air inlet pipe. When the piston moves to the side close to the purification tank, the gas in the cylinder can be injected into the purification tank through the air outlet pipe. The purification tank can be continuously pressurized, thereby avoiding the situation where the elution speed is slowed down due to the outflow of liquid and the loss of air, thereby improving the elution and purification rate of the polypeptide. In addition, the bidirectional lead screw is jointly driven by the driving motor that drives the spiral feeding rod to rotate, thereby avoiding the need to set up a separate driving device to drive the bidirectional lead screw to rotate, thereby reducing the manufacturing and use costs of the pressurizing component and the polypeptide separation and purification system.

[0021] Preferably, the indicating assembly comprises an annular fixing plate mounted on the inner side wall at the top of the purification tank, the fixing frame is elastically connected to the top of the annular mounting seat, a piezoelectric ceramic sheet corresponding to the fixing frame is mounted on the bottom wall of the annular fixing plate, and a plurality of indicator lights electrically connected to the piezoelectric ceramic sheet are mounted on the outer side wall of the purification tank.

[0022] The filtered components are adsorbed in the screen, causing the filtering effect of the screen to deteriorate, and the resistance of the screen to the liquid will become greater. When the liquid hits the screen from the bottom of the screen, the screen, the mounting frame and the fixed frame will move upward and push the fixed frame to squeeze the piezoelectric ceramic piece upward. After the piezoelectric ceramic piece is squeezed, it will generate current and power the indicator light. After the indicator light is powered on, it will give an indication and remind you to replace the screen in time. The screen can be replaced in time at the appropriate time, which not only reduces the labor intensity of manual labor and avoids the waste of materials such as adsorbents, but also further improves the purification quality of polypeptides.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The filtered components are adsorbed in the screen, causing the filtering effect of the screen to deteriorate. The resistance of the screen to the liquid will increase. When the liquid impacts the screen from the bottom of the screen, the screen, the mounting frame and the fixed frame will move upward and push the fixed frame upward to squeeze the piezoelectric ceramic piece. After the piezoelectric ceramic piece is squeezed, it will generate current and power the indicator light. After the indicator light is powered on, it will give an indication and remind you to replace the screen in time. The screen can be replaced in time at the appropriate time, which not only reduces the labor intensity of manual labor and avoids the waste of materials such as adsorbents, but also further improves the purification quality of polypeptides.

[0025] 2. The components filtered by the screen all fall to the bottom of the screen due to their own weight, which not only facilitates cleaning from the bottom of the purification tank, but also improves the screening effect of the screen, thereby improving the purification quality of the polypeptide.

[0026] 3. The mixing mechanism can mix and stir the polypeptide solution and the eluent added to the purification tank, thereby improving the elution effect of the polypeptide solution and further improving the purification quality of the polypeptide.

[0027] 4. The pressurizing component can continuously pressurize the purification tank, thus avoiding the situation where the elution speed slows down due to the outflow of liquid and the loss of air, thereby improving the elution and purification rate of the polypeptide. In addition, the pressurizing component is jointly driven by the mixing mechanism, thus avoiding the need to set up a separate driving device to drive the pressurizing component, thereby reducing the manufacturing and use costs of the pressurizing component and the polypeptide separation and purification system. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 It is a side structural schematic diagram of the present invention;

[0030] Figure 3 It is a side structural cross-sectional view of the present invention;

[0031] Figure 4It is a top view of the fixing frame part of the present invention when the screen is not installed;

[0032] Figure 5 This is a top view of the fixing frame of the present invention after the screen is installed;

[0033] Figure 6 It is an enlarged view of the structure of part A of the present invention.

[0034] In the figure: 1. purification tank; 2. support frame; 3. liquid adding tank; 4. liquid outlet pipe; 5. installation frame; 6. screen; 7. cylinder; 8. sealing bottom cover; 9. annular sealing plate; 10. annular sealing groove; 11. sealing gasket; 12. installation rod; 13. annular mounting seat; 14. fixing frame; 15. annular positioning plate; 16. connecting plate; 17. fixing seat; 18. connecting groove; 19. limiting plug block; 20. limiting jack; 21. push rod; 22. mixing tube; 23. spiral feeding rod; 24. liquid inlet pipe; 25. driving motor; 26. fixing frame; 27. bidirectional screw rod; 28. slider; 29. ​​transmission belt; 30. cylinder body; 31. piston; 32. outlet pipe; 33. annular fixing plate; 34. piezoelectric ceramic sheet; 35. indicator light; 36. nozzle; 37. cleaning liquid pipe. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0037] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined. In addition, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0038] See also Figures 1 to 6 The present invention provides a polypeptide separation and purification system, and the technical scheme is as follows:

[0039] A polypeptide separation and purification system comprises a purification tank 1, a support frame 2 is installed at the bottom end of the purification tank 1, a liquid adding tank 3 and a liquid outlet pipe 4 are connected to the outer side walls of the bottom end and the top end of the purification tank 1 respectively, a mounting frame 5 is detachably installed at the middle end of the purification tank 1, a screen 6 is installed on the inner side wall of the mounting frame 5, and the screen 6 is located between the liquid adding tank 3 and the liquid outlet pipe 4, two cylinders 7 are symmetrically installed on the outer side wall of the purification tank 1, and a sealed bottom cover 8 that cooperates with the purification tank 1 is installed at the output ends of the two cylinders 7, and the mounting frame 5 is detachably connected to the top end of the sealed bottom cover 8, and a positioning group is provided between the sealed bottom cover 8 and the bottom end of the purification tank 1. A polypeptide storage tank and an eluent tank are connected to the liquid adding tank 3, a mixing mechanism is connected between the liquid adding tank 3 and the purification tank 1, a pressurizing component is connected to the bottom end of the purification tank 1, and the pressurizing component is driven by the mixing mechanism. An indicating component cooperating with the screen 6 is provided in the purification tank 1, and the indicating component is used to timely remind the replacement of the screen 6 with poor screening effect. A nozzle 36 is installed on the inner side wall of the top of the purification tank 1, and a cleaning liquid pipe 37 is connected to the top of the nozzle 36. After the nozzle 36 sprays the cleaning liquid from the top of the purification tank 1, the inner side wall of the purification tank 1 can be easily cleaned, thereby reducing the cleaning difficulty of the purification tank 1.

[0040] The liquid adding tank 3 injects the polypeptide solution and the eluent that cooperates with the polypeptide solution into the purification tank 1 through the bottom of the purification tank 1. The eluted polypeptide solution is purified by the screen 6 and then discharged through the liquid outlet pipe 4 at the top. The components filtered by the screen 6 fall to the bottom of the screen 6 due to their own weight, which is not only convenient for cleaning from the bottom of the purification tank 1, but also improves the screening effect of the screen 6, thereby improving the purification quality of the polypeptide; the mixing mechanism can mix and stir the polypeptide solution and the eluent added to the purification tank 1, improve the elution effect of the polypeptide solution, thereby further improving the purification quality of the polypeptide; the pressurizing component can continuously pressurize the purification tank 1, so as to avoid the situation where the elution speed is slowed down due to the outflow of liquid and the loss of air, thereby improving the elution and purification rate of the polypeptide; the pressurizing component is jointly driven by the mixing mechanism, so it can avoid the need to set up a separate driving device to drive the pressurizing component, thereby reducing the manufacturing and use costs of the pressurizing component and the polypeptide separation and purification system.

[0041] As an embodiment of the present invention, refer to Figure 3 , Figure 4 , Figure 5 and Figure 6 An annular sealing plate 9 is installed on the top wall of the sealing bottom cover 8, and an annular sealing groove 10 that cooperates with the annular sealing plate 9 is provided at the bottom end of the purification tank 1. A sealing gasket 11 that cooperates with the annular sealing plate 9 is installed on the inner side wall of the annular sealing groove 10, which improves the sealing performance of the connection between the sealing bottom cover 8 and the purification tank 1. A plurality of mounting rods 12 are installed on the top of the sealing bottom cover 8, and an annular mounting seat 13 is commonly installed on the top of the mounting rod 12. A fixing frame 14 is installed on the top of the annular mounting seat 13, and a threaded hole that meshes with the mounting frame 5 is provided on the inner side wall of the fixing frame 14. An annular positioning plate 15 that cooperates with the mounting frame 5 is installed on the inner side wall of the bottom end of the fixing frame 14, which improves the stability and sealing performance of the connection between the mounting frame 5 and the fixing frame 14. A plurality of protrusions are installed on the top wall of the mounting frame 5 to facilitate the rotation of the mounting frame 5, thereby facilitating the threaded connection of the mounting frame 5 and the screen 6 to the fixing frame 14 or removal from the fixing frame 14.

[0042] Starting the cylinder 7 can drive the sealed bottom cover 8 to drive the mounting rod 12, the annular mounting seat 13 and the fixed frame 14 to move up and down. By moving the fixed frame 14 to the bottom end of the purification tank 1, the mounting frame 5 and the screen 6 can be conveniently threadedly connected to the fixed frame 14 or removed from the fixed frame 14. By moving the fixed frame 14 into the purification tank 1 and attaching the sealed bottom cover 8 to the bottom end of the purification tank 1, the mounting frame 5 and the screen 6 can be installed in the purification tank 1. The screen 6 can be conveniently installed and removed, and the screen 6 has good stability after installation, which can not only avoid the large noise caused by the impact of the liquid on the screen 6, but also significantly improve the purification quality of the polypeptide. A control valve is arranged in the sealed bottom cover 8. When the purification tank 1 needs to be drained, the remaining liquid in the purification tank 1 can be discharged by opening the control valve.

[0043] As an embodiment of the present invention, refer to Figure 3 and Figure 6 The positioning assembly includes two connecting plates 16 symmetrically mounted on the top wall of the sealing bottom cover 8, two fixing seats 17 are symmetrically mounted on the outer side wall of the bottom end of the purification tank 1, and the bottom ends of the two fixing seats 17 are provided with connecting grooves 18 that cooperate with the connecting plates 16. The outer side walls of the two connecting plates 16 are elastically mounted with limiting plugs 19, and the inner side walls of the two connecting grooves 18 are provided with limiting plug holes 20 that cooperate with the limiting plugs 19. The top walls of one end of the two limiting plugs 19 are both inclined surfaces, and the limiting plugs 1 After the inclined surface of 9 is squeezed, it will push the limiting plug 19 to shrink to the inner side of the connecting plate 16, so as to facilitate the upward movement of the connecting plate 16 and the limiting plug 19. The outer side walls of the two fixing seats 17 are threadedly connected with push rods 21 that cooperate with the limiting plug 19, and one end of the push rod 21 extends into the corresponding limiting socket 20. By rotating the push rod 21 and pushing the limiting plug 19 to the outer side of the limiting socket 20, the limiting plug 19, the connecting plate 16 and the sealing bottom cover 8 can be easily removed from the bottom end of the purification tank 1.

[0044] When the sealed bottom cover 8 moves upward, the connecting plate 16 moves upward accordingly. When the sealed bottom cover 8 is tightly attached to the bottom end of the purification tank 1, the two connecting plates 16 are respectively inserted into the connecting grooves 18 at the bottom ends of the two fixing seats 17. At this time, the two limiting plugs 19 are pushed into the corresponding limiting plug holes 20 by elastic force. Therefore, the limiting plugs 19, the connecting plates 16 and the sealed bottom cover 8 can be fixedly connected to the bottom end of the purification tank 1, thereby improving the installation stability and sealing of the sealed bottom cover 8. Therefore, it can be avoided that the sealed bottom cover 8 loosens when pressurized into the purification tank 1, resulting in poor sealing of the purification tank 1, thereby improving the stability of the purification tank 1 and the polypeptide separation and purification system during processing.

[0045] As an embodiment of the present invention, refer to Figure 2 and Figure 3 The mixing mechanism includes a mixing tube 22, and the liquid adding tank 3 is fixed on the top wall at one end of the mixing tube 22. A spiral feeding rod 23 is rotatably installed in the mixing tube 22. A liquid inlet pipe 24 is connected between the mixing tube 22 and the purification tank 1. A one-way liquid inlet valve is installed in the liquid inlet pipe 24 to prevent the liquid from flowing back into the mixing tube 22. A driving motor 25 is installed at one end of the mixing tube 22 away from the liquid inlet pipe 24, and one end of the spiral feeding rod 23 is connected to the output end of the driving motor 25.

[0046] The liquid adding tank 3 injects the polypeptide solution and the eluent that cooperates with the polypeptide solution into the mixing tube 22 via the polypeptide storage tank and the eluent tank. Starting the driving motor 25 can drive the spiral feeding rod 23 to rotate. The rotation of the spiral feeding rod 23 can fully mix and stir the polypeptide solution and the eluent in the mixing tube 22 and evenly transport them to the bottom end of the purification tank 1 through the liquid inlet pipe 24, thereby improving the elution effect of the polypeptide solution, thereby further improving the purification quality of the polypeptide.

[0047] As an embodiment of the present invention, refer to Figure 2 and Figure 3 The pressurizing assembly includes a fixing frame 26 fixed at the bottom end of the mixing tube 22, a bidirectional screw rod 27 is horizontally rotatably installed on the fixing frame 26, a slider 28 is threadedly connected to the bidirectional screw rod 27, a transmission belt 29 is installed at one end of the bidirectional screw rod 27 adjacent to the spiral feeding rod 23, a cylinder body 30 is provided at one end of the bidirectional screw rod 27, a piston 31 is installed in the cylinder body 30, two fixing rods are symmetrically installed at one end of the piston 31, and one end of the fixing rods is connected to the slider 28, an air inlet pipe and an air outlet pipe 32 are installed at one end of the cylinder body 30, a one-way air valve is installed on the air inlet pipe and the air outlet pipe 32, and one end of the air outlet pipe 32 is connected to the outer wall of the bottom end of the purification tank 1, the one-way air valve can prevent the liquid in the purification tank 1 from flowing into the cylinder body 30, and a safety valve is installed on the purification tank 1 to avoid safety accidents caused by excessive pressure in the purification tank 1.

[0048] The driving motor 25 drives the spiral feeding rod 23 to rotate, which can drive the transmission belt 29 and the bidirectional screw rod 27 to rotate. The rotation of the bidirectional screw rod 27 can drive the slider 28 and the piston 31 to reciprocate in the horizontal direction. When the piston 31 moves to the side away from the purification tank 1, the gas can be sucked into the cylinder body 30 through the air inlet pipe. When the piston 31 moves to the side close to the purification tank 1, the gas in the cylinder body 30 can be injected into the purification tank 1 through the air outlet pipe 32, and the purification tank 1 can be continuously pressurized. Therefore, the situation in which the elution speed is slowed down due to the outflow of liquid and the loss of air can be avoided, thereby improving the elution and purification rate of the polypeptide. In addition, the bidirectional screw rod 27 is jointly driven by the driving motor 25 that drives the spiral feeding rod 23 to rotate, so it is possible to avoid setting up a separate driving device to drive the bidirectional screw rod 27 to rotate, thereby reducing the manufacturing and use costs of the pressurizing component and the polypeptide separation and purification system.

[0049] As an embodiment of the present invention, refer to Figure 3 The indicating component includes an annular fixing plate 33 installed on the inner wall of the top of the purification tank 1, the fixing frame 14 is elastically connected to the top of the annular mounting seat 13, a piezoelectric ceramic piece 34 corresponding to the fixing frame 14 is installed on the bottom wall of the annular fixing plate 33, and a plurality of indicator lights 35 electrically connected to the piezoelectric ceramic piece 34 are installed on the outer wall of the purification tank 1.

[0050] The filtered components are adsorbed in the screen 6, resulting in the deterioration of the filtering effect of the screen 6. The resistance of the screen 6 to the liquid will become larger. When the liquid impacts the screen 6 from the bottom end of the screen 6, the screen 6, the mounting frame 5 and the fixed frame 14 will all move upward and push the fixed frame 14 to squeeze the piezoelectric ceramic sheet 34 upward. After the piezoelectric ceramic sheet 34 is squeezed, it will generate current and supply power to the indicator light 35. After the indicator light 35 is powered on, it will give an indication and promptly remind you to replace the screen 6. The screen 6 can be replaced in time at an appropriate time, which not only reduces the labor intensity of manual labor and avoids the waste of materials such as adsorbents, but also further improves the purification quality of the polypeptide.

[0051] Working principle: the liquid adding tank 3 injects the polypeptide solution and the eluent that cooperates with the polypeptide solution into the mixing tube 22 through the polypeptide storage tank and the eluent tank, and the driving motor 25 is started to drive the spiral feeding rod 23 to rotate. The rotation of the spiral feeding rod 23 can fully mix and stir the polypeptide solution and the eluent in the mixing tube 22 and evenly transport them to the bottom end of the purification tank 1 through the liquid inlet pipe 24. The eluted polypeptide solution is purified by the screen 6 and then discharged through the liquid outlet pipe 4 at the top. The components filtered by the screen 6 all fall to the bottom end of the screen 6 due to their own weight, which is not only convenient for cleaning from the bottom end of the purification tank 1, but also improves the screening effect of the screen 6; the driving motor 25 drives the spiral feeding rod 23 to rotate, which can drive the transmission belt 29 and the bidirectional screw 27 to rotate. The rotation of the bidirectional screw 27 can drive the slider 28 and the piston 31 to reciprocate in the horizontal direction. When the piston 31 moves to the side away from the purification tank 1, the gas can be sucked into the cylinder body 30 through the air inlet pipe. When the piston 31 moves toward the side close to the purification tank 1, the gas in the cylinder 30 can be injected into the purification tank 1 through the air outlet pipe 32, and the purification tank 1 can be continuously pressurized, so the situation that the elution speed is slowed down due to the outflow of liquid and the loss of air can be avoided, thereby improving the elution and purification rate of the polypeptide; after the filtered components are adsorbed in the screen 6, the filtering effect of the screen 6 becomes worse, and the resistance of the screen 6 to the liquid will become larger. When the liquid impacts the screen 6 from the bottom end of the screen 6, the screen 6, the installation frame 5 and the fixed frame 14 will all move upward and push the fixed frame 14 to squeeze the piezoelectric ceramic sheet 34 upward. After the piezoelectric ceramic sheet 34 is squeezed, it will generate current and power the indicator light 35. After the indicator light 35 is powered on, it will give an indication and remind you to replace the screen 6 in time. The screen 6 can be replaced in time at an appropriate time, which not only reduces the labor intensity of manual labor and avoids the waste of materials such as adsorbents, but also further improves the purification quality of the polypeptide.

[0052] The electrical components appearing in this article are all connected to the external main controller and 220V AC power through a transformer, and the main controller can be a conventional known device that controls a computer, etc. The product model provided by the present invention is only used for the technical solution according to the structural characteristics of the product. The product will be adjusted and modified after purchase to make it more matching and consistent with the technical solution of the present invention. It is a technical solution for the best application of the technical solution. The model of its product can be replaced and modified according to the required technical parameters, which is well known to technical personnel in this field. Therefore, technical personnel in this field can clearly obtain the corresponding use effect through the technical solution provided by the present invention.

[0053] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A polypeptide separation and purification system, comprising a purification tank (1), wherein a support frame (2) is installed at the bottom end of the purification tank (1), characterized in that: The outer side walls of the bottom and top ends of the purification tank (1) are connected to a liquid adding tank (3) and a liquid outlet pipe (4) respectively; a mounting frame (5) is detachably mounted on the middle end of the purification tank (1); a screen (6) is mounted on the inner side wall of the mounting frame (5); the screen (6) is located between the liquid adding tank (3) and the liquid outlet pipe (4); an adsorbent is fixed on the screen (6); two cylinders (7) are symmetrically mounted on the outer side wall of the purification tank (1); a sealed bottom cover (8) that cooperates with the purification tank (1) is commonly mounted at the output ends of the two cylinders (7); and the mounting frame (5) is detachably connected to the top end of the sealed bottom cover (8); The sealing bottom cover (8) and the bottom end of the purification tank (1) are provided with a positioning assembly, the liquid adding tank (3) is connected to a polypeptide storage tank and an eluent tank, a mixing mechanism is connected between the liquid adding tank (3) and the purification tank (1), the bottom end of the purification tank (1) is connected to a pressurizing assembly, and the pressurizing assembly is driven by the mixing mechanism, an indicating assembly that cooperates with the screen (6) is provided in the purification tank (1), and the indicating assembly is used to promptly remind the replacement of the screen (6) with a poor screening effect, a nozzle (36) is installed on the inner side wall of the top end of the purification tank (1), and the top end of the nozzle (36) is connected to a cleaning liquid pipe (37); The mixing mechanism comprises a mixing tube (22), and the liquid adding tank (3) is fixed on the top wall of one end of the mixing tube (22), a spiral feeding rod (23) is rotatably installed in the mixing tube (22), a liquid inlet pipe (24) is connected between the mixing tube (22) and the purification tank (1), a one-way liquid inlet valve is installed in the liquid inlet pipe (24), a driving motor (25) is installed at one end of the mixing tube (22) away from the liquid inlet pipe (24), and one end of the spiral feeding rod (23) is connected to the output end of the driving motor (25); The pressurizing assembly comprises a fixing frame (26) fixed to the bottom end of the mixing tube (22), a bidirectional screw rod (27) being horizontally rotatably mounted on the fixing frame (26), a slider (28) being threadedly connected to the bidirectional screw rod (27), a transmission belt (29) being installed at one end of the bidirectional screw rod (27) adjacent to the spiral feeding rod (23), a cylinder body (30) being provided at one end of the bidirectional screw rod (27), a piston (31) being installed in the cylinder body (30), two fixing rods being symmetrically mounted at one end of the piston (31), and one end of each of the fixing rods being connected to the slider (28), an air inlet pipe and an air outlet pipe (32) being mounted at one end of the cylinder body (30), one-way air valves being installed on each of the air inlet pipe and the air outlet pipe (32), and one end of the air outlet pipe (32) being connected to the outer side wall of the bottom end of the purification tank (1); An annular sealing plate (9) is installed on the top wall of the sealing bottom cover (8); an annular sealing groove (10) cooperating with the annular sealing plate (9) is provided at the bottom end of the purification tank (1); a sealing gasket (11) cooperating with the annular sealing plate (9) is installed on the inner side wall of the annular sealing groove (10); a plurality of mounting rods (12) are installed at the top end of the sealing bottom cover (8); an annular mounting seat (13) is commonly installed at the top end of the mounting rods (12); a fixing frame (14) is installed at the top end of the annular mounting seat (13); a threaded hole meshing with the mounting frame (5) is provided on the inner side wall of the fixing frame (14); and an annular positioning plate (15) cooperating with the mounting frame (5) is installed on the inner side wall of the bottom end of the fixing frame (14); The indicating assembly comprises an annular fixing plate (33) mounted on the inner side wall of the top end of the purification tank (1); the fixing frame (14) is elastically connected to the top end of the annular mounting seat (13); a piezoelectric ceramic sheet (34) corresponding to the fixing frame (14) is mounted on the bottom wall of the annular fixing plate (33); and a plurality of indicator lights (35) electrically connected to the piezoelectric ceramic sheets (34) are mounted on the outer side wall of the purification tank (1).

2. A polypeptide separation and purification system according to claim 1, characterized in that: The positioning assembly comprises two connecting plates (16) symmetrically mounted on the top wall of the sealing bottom cover (8); two fixing seats (17) are symmetrically mounted on the outer side wall of the bottom end of the purification tank (1); the bottom ends of the two fixing seats (17) are provided with connecting grooves (18) that cooperate with the connecting plates (16); limiting plug blocks (19) are elastically mounted on the outer side walls on the opposite sides of the two connecting plates (16); limiting plug holes (20) that cooperate with the limiting plug blocks (19) are disposed on the inner side walls of the two connecting grooves (18); push rods (21) that cooperate with the limiting plug blocks (19) are threadedly connected to the outer side walls of the two fixing seats (17), and one end of the push rods (21) extends into the corresponding limiting plug holes (20).

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