Method for accelerating the formation of aerobic granular sludge using magnetic powder-enhanced active mycelium balls

Abstract

The present invention discloses a method for accelerating the formation of aerobic granular sludge by using magnetic powder-enhanced active mycelial pellets, the method comprising the following steps: 1) inoculating fungal spores in a culture medium, culturing and obtaining a fungal suspension; 2) adding magnetic powder to the fungal suspension for co-culturing to obtain magnetic powder-enhanced active mycelial pellets; 3) inoculating the magnetic powder-enhanced active mycelial pellets in a reactor; 4) operating the reactor in a batch mode to treat wastewater, culturing and obtaining the aerobic granular sludge; wherein the fungi include at least one of Aspergillus polydorum and Cladosporium microsporum. The present invention directly adds magnetic powder-enhanced active mycelial pellets with pollutant removal capabilities in the early stage of reactor startup, which can effectively avoid the problem of low organic matter removal rate due to the inadaptability of activated sludge in the early stage of reactor startup, ensure the effluent quality in the startup period, and significantly accelerate the formation of aerobic granular sludge.

Description

Technical Field

[0001] The invention relates to the technical field of biological sewage treatment, and in particular to a method for accelerating the formation of aerobic granular sludge by using magnetic powder-enhanced active mycelium balls. Background Art

[0002] Aerobic granular sludge (AGS) is a dense biopolymer with regular appearance and compact structure formed by microorganisms in sludge through self-flocculation. Since its discovery in the 1990s, aerobic granular sludge technology has shown great potential to replace traditional activated sludge with its excellent sedimentation performance. Aerobic granular sludge technology also has the advantages of dense particle structure, high biomass concentration, high biomass retention rate, and high stress resistance (such as toxic compounds, salinity, and shock load). However, the long granulation time is one of the bottleneck problems that limit the practical application of aerobic granular sludge technology.

[0003] At present, the main methods to accelerate the formation of granular sludge include adjusting operating parameters, adding metal ions, adding inert carriers or flocculants, inoculating granular sludge, and inoculating special strains. Adjusting operating parameters is the basic means to accelerate the formation of AGS. It is generally believed that high organic load, high hydraulic shear, and short sedimentation time are conducive to the rapid formation of AGS. However, in actual wastewater treatment processes, especially in the treatment of difficult-to-degrade industrial wastewater, if the effluent quality during the startup period needs to be taken into account, there are many limitations to simply adjusting operating parameters to accelerate granulation. Continuous addition of metal ions is not suitable for large-scale wastewater treatment processes, and it also increases the burden of deep sewage treatment. The addition of inert carriers and flocculants increases the cost of sludge disposal to a certain extent. The feasibility of the strategy of adding granular sludge depends on the cost of granular sludge and the transportation distance. Therefore, it is necessary to study the bioaugmentation technology of inoculating special strains so that they can be produced as microbial preparations and added during the startup period of AGS reactors.

[0004] Mycelial balls have the advantages of fast sedimentation, easy solid-liquid separation, high biological activity, strong adsorption capacity for pollutants, and the ability to secrete a variety of enzymes. When mycelial balls are added to activated sludge, they can serve as core carriers, provide space for microorganisms to adsorb and grow, promote the aggregation of microorganisms, and thus accelerate the formation of granular sludge. However, this technology currently still has problems such as mycelial balls being easily broken and floating.

[0005] The prior art discloses a variety of methods for accelerating aerobic sludge granulation, but there are still problems such as high cost, unstable granulation process, and long granulation time.

[0006] For example, the prior art application number is 202111530721.X, entitled "A method for adding ferroferric oxide magnetic nanoparticles to enhance sludge granulation". The addition of ferroferric oxide magnetic nanoparticles as inducing nuclei for particle formation shortens the time for aerobic granular sludge formation. However, this technology requires the regular addition of ferroferric oxide magnetic nanoparticle mother liquor to the reactor, which significantly increases the cost.

[0007] For example, the prior art application number is 201610549254.8, entitled "A method for promoting aerobic sludge granulation by mycelial balls", which uses mycelial balls as inducing crystal nuclei to attach and fix aerobic activated sludge on the surface of mycelium to form densely structured granular sludge. However, this technology has the problem that mycelial balls are easy to break and float, and the granulation process is unstable.

[0008] For example, the prior art application number is 202111263435.1, entitled "A method for rapid cultivation of aerobic granular sludge for treating high-salt wastewater". By adding a sugar carbon source in the early stage, the formation of mycelial balls is induced, the start-up of salt-tolerant aerobic granular sludge is accelerated, and aerobic granular sludge that tolerates salinity is cultivated quickly and at low cost. This technology induces mycelial balls by adding a carbon source, but the mycelial ball species have poor repeatability and are difficult to maintain consistency in different batches.

[0009] For example, the prior art application number is 201611181910.X, entitled "A preparation method and application of nano-silicon fungus-type magnetic aerobic granular sludge", which prepares magnetic nanospheres of ferroferric oxide / silicon dioxide, and adds them together with fungi to the granular sludge system to prepare modified aerobic granular sludge for wastewater treatment. This technology uses magnetic nanospheres of ferroferric oxide / silicon dioxide to fix microbial cells, and the resulting material has a large volume and density. To achieve uniform mixing, a large amount of aeration needs to be applied, and the actual process is more inclined to the biofilm method rather than the aerobic granular sludge method. The wastewater treatment effect is more dependent on the initial material dosage, and the material production cost is high. Summary of the invention

[0010] In view of the problems existing in the prior art, in the first aspect, the purpose of the present invention is to provide an aerobic granular sludge, wherein the aerobic granular sludge is obtained by adding magnetic powder-enhanced active mycelial pellets for cultivation. In the second aspect, the purpose of the present invention is to provide a use of aerobic granular sludge, wherein the aerobic granular sludge is obtained by adding magnetic powder-enhanced active mycelial pellets for cultivation. In the third aspect, the purpose of the present invention is to provide a method for accelerating the formation of aerobic granular sludge by using magnetic powder-enhanced active mycelial pellets, or to provide a method for preparing aerobic granular sludge, wherein the aerobic granular sludge is obtained by adding magnetic powder-enhanced active mycelial pellets for cultivation.

[0011] The present invention adopts the following technical solutions:

[0012] In a first aspect, the present application provides an aerobic granular sludge cultured with magnetic powder-enhanced active mycelial pellets, wherein the aerobic granular sludge is prepared by a method comprising the following steps:

[0013] 1) inoculating fungal spores in a culture medium, culturing and obtaining a fungal suspension;

[0014] 2) adding magnetic powder to the fungal suspension for co-cultivation to obtain magnetic powder-enhanced active mycelium pellets;

[0015] 3) inoculating the magnetic powder enhanced active mycelium pellets in a reactor;

[0016] 4) operating the reactor in a batch mode to treat wastewater, and cultivating and obtaining the aerobic granular sludge;

[0017] The fungus comprises at least one of Aspergillus polydorum and Cladosporium exigua; the fungus consists of at least one of Aspergillus polydorum and Cladosporium exigua. For example, the fungus is Aspergillus polydorum; alternatively, the fungus is Cladosporium exigua.

[0018] Without wishing to be bound by theory, in some feasible embodiments, the name of the Aspergillus sydowii is Aspergillus sydowii NXY1, which was deposited in the China General Microbiology Center (CGMCC) on June 17, 2022, with a deposit number of CGMCC No: 40215.

[0019] Without wishing to be bound by theory, in some feasible embodiments, the name of the Cladosporium tenuissimum is Cladosporium tenuissimum NXY8, which was deposited in the China General Microbiology Center (CGMCC) on March 4, 2022, with a deposit number of CGMCC No: 40141.

[0020] In combination with the first aspect, in some feasible embodiments, the present application further provides a magnetic powder-reinforced active mycelium pellet, wherein the magnetic powder-reinforced active mycelium pellet is prepared by a method comprising the following steps:

[0021] 1) inoculating fungal spores in a culture medium, culturing and obtaining a fungal suspension;

[0022] 2) adding magnetic powder to the fungal suspension for co-cultivation to obtain magnetic powder-reinforced active mycelium pellets.

[0023] In combination with the first aspect, in some feasible embodiments, the fungus can tolerate a salinity range of up to 10%, for example 0% to 10%, such as 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0024] In combination with the first aspect, in some feasible embodiments, in step 1), the inoculation amount of fungal spores is 1×10 2 Up to 1×10 7 spores / mL medium, for example, about 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 spores / mL culture medium, etc., but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0025] In combination with the first aspect, in some feasible embodiments, in step 1), the inoculation amount of fungal spores is 1×10 2 Up to 1×10 7 spores / g medium, for example, about 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 spores / g culture medium, etc., but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0026] In combination with the first aspect, in some feasible embodiments, in step 1), the culture medium is a sterile culture medium.

[0027] In combination with the first aspect, in some feasible embodiments, in step 1), the culture medium is a liquid culture medium.

[0028] In combination with the first aspect, in some feasible embodiments, in step 1), the culture medium contains or consists of the following items: 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH 4 Cl, 0.3g / L to 6g / L KH 2 PO 4 , 0.25g / L to 4g / LMgSO 4 7H 2 0, 0g / L to 100g / LNaCl; Optionally, the glucose is anhydrous glucose.

[0029] In combination with the first aspect, in some feasible embodiments, in step 1), the culture medium contains or consists of the following items: 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH 4 Cl, 0.3g / L to 6g / L KH 2 PO 4 , 0.25g / L to 4g / LMgSO 4 7H 2 O, 0g / L to 100g / LNaCl, add water to make up to 1L; optionally, the glucose is anhydrous glucose.

[0030] In combination with the first aspect, in some feasible embodiments, in step 2), magnetic powder is added to the fungal suspension during any one of the spore stage, hyphae emergence stage, hyphae aggregation stage, and hyphae ball maturity stage of the fungus;

[0031] In combination with the first aspect, in some feasible embodiments, in step 2), the size of the magnetic powder is 3 μm to 100 μm, for example 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52 , 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 μm, etc., but are not limited to the listed values, and other values ​​or ranges not listed within the numerical range are also applicable.

[0032] In combination with the first aspect, in some feasible embodiments, in step 2), the mass of the added magnetic powder accounts for 1% to 50% of the mass of the magnetic powder-enhanced active mycelial ball, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, %, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, and the like, but are not limited to the listed values, and other values ​​or ranges not listed within the numerical range are also applicable.

[0033] In combination with the first aspect, in some feasible embodiments, in step 2), the culture medium is prepared after high-pressure sterilization.

[0034] In combination with the first aspect, in some feasible embodiments, in step 2), the fungal suspension is cultured at 15°C to 35°C, for example, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35°C, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0035] In combination with the first aspect, in some feasible embodiments, in step 2), the culture condition of the fungal suspension is 60 rpm to 400 rpm, for example, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400 rpm, and the like, but is not limited to the listed values, and other values ​​or ranges not listed within the numerical range are equally applicable.

[0036] In combination with the first aspect, in some feasible embodiments, in step 2), the culturing condition of the fungal suspension is constant temperature culturing for 20 h to 240 h, for example, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240 h, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0037] In combination with the first aspect, in some feasible embodiments, in step 3), the inoculation amount of the magnetic powder-enhanced active mycelium ball is 0.1 g / L to 10 g / L, for example, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 g / L, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within this numerical range are also applicable.

[0038] In combination with the first aspect, in some feasible implementation schemes, in step 3), the magnetic powder enhanced active mycelium balls and activated sludge are inoculated in the reactor; the mass ratio of the activated sludge to the magnetic powder enhanced active mycelium balls is 0 to 90:1, for example 0:1, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0039] In combination with the first aspect, in some feasible implementation schemes, in step 4), the sequencing batch operation process includes water inlet, aeration, static sedimentation, water outlet and idle stages.

[0040] In combination with the first aspect, in some feasible implementation schemes, in step 4), the source of wastewater includes at least one of domestic wastewater, municipal wastewater, food industry wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater, printing and dyeing wastewater, animal husbandry wastewater and prepared simulated wastewater.

[0041] In combination with the first aspect, in some feasible implementation schemes, in step 4), when wastewater is used as influent, the organic load range of the reaction system is 0.3 kg COD / (m 3 ·day) to 25kg COD / (m 3 ·day), for example 0.3, 0.5, 0.8, 1.2, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 kg COD / (m 3 ·days), etc., but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0042] In combination with the first aspect, in some feasible embodiments, the time for aerobic granular sludge formation is 1 to 20 days, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 days, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0043] In combination with the first aspect, in some feasible implementation schemes, in step 4), the total time of each operation cycle of the batch operation is 1 hour to 24 hours, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 hours, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0044] In combination with the first aspect, in some feasible embodiments, in step 4), the pH of the reaction system is maintained at 5 to 9 during batch operation, for example 5, 6, 7, 8, 9, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0045] In combination with the first aspect, in some feasible embodiments, in step 4), the reaction temperature during batch operation is maintained at 15°C to 35°C, for example, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35°C, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0046] In combination with the first aspect, in some feasible embodiments, in step 4), the volume exchange rate per cycle during batch operation is maintained at 30% to 80%, for example, 30%, 40%, 50%, 60%, 70%, 80%, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0047] In combination with the first aspect, in some feasible implementation schemes, in step 4), during the aeration stage of sequencing batch operation, air enters from the bottom, and the superficial gas velocity (the ratio of air flow rate to the cross-sectional area in the reactor) is maintained at 0.1 cm / s to 4.5 cm / s, for example, 0.1, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 4.5 cm / s; the dissolved oxygen concentration is controlled at 2 mg / L to 10 mg / L, for example, 2, 3, 4, 5, 6, 7, 8, 9, 10 mg / L, etc., but is not limited to the listed values, and other values ​​or ranges not listed within the numerical range are equally applicable.

[0048] In combination with the first aspect, in some feasible implementation schemes, in step 4), the time range of the static sedimentation stage during batch operation is 0.1 min to 30 min, for example, 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 min, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are equally applicable.

[0049] In the second aspect, the use of aerobic granular sludge is provided, wherein the use of the aerobic granular sludge for treating wastewater can reduce harmful or polluting components in the wastewater, for example, achieving a better COD degradation rate, wherein the aerobic granular sludge is obtained by adding magnetic powder-enhanced active mycelium ball culture.

[0050] In combination with the second aspect, in some feasible implementation schemes, the source of the wastewater includes at least one of domestic wastewater, municipal wastewater, food industry wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater, printing and dyeing wastewater, animal husbandry wastewater, and prepared simulated wastewater.

[0051] In a third aspect, a method for accelerating the formation of aerobic granular sludge using magnetic powder-enhanced active mycelium pellets is provided, comprising the following steps:

[0052] 1) inoculating fungal spores in a culture medium, culturing and obtaining a fungal suspension;

[0053] 2) adding magnetic powder to the fungal suspension for co-cultivation to obtain magnetic powder-enhanced active mycelium pellets;

[0054] 3) inoculating the magnetic powder enhanced active mycelium pellets in a reactor;

[0055] 4) operating the reactor in a batch mode to treat wastewater, and cultivating and obtaining the aerobic granular sludge;

[0056] The fungus comprises at least one of Aspergillus polydorum and Cladosporium exigua; the fungus consists of at least one of Aspergillus polydorum and Cladosporium exigua. For example, the fungus is Aspergillus polydorum; alternatively, the fungus is Cladosporium exigua.

[0057] Without wishing to be bound by theory, in some feasible embodiments, the name of the Aspergillus sydowii is Aspergillus sydowii NXY1, which was deposited in the China General Microbiology Center (CGMCC) on June 17, 2022, with a deposit number of CGMCC No: 40215.

[0058] Without wishing to be bound by theory, in some feasible embodiments, the name of the Cladosporium tenuissimum is Cladosporium tenuissimum NXY8, which was deposited in the China General Microbiology Center (CGMCC) on March 4, 2022, with a deposit number of CGMCC No: 40141.

[0059] In combination with the third aspect, in some feasible embodiments, the fungus can tolerate a salinity range of up to 10%, for example 0% to 10%, such as 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0060] In conjunction with the third aspect, in some feasible embodiments, in step 1), the inoculation amount of fungal spores is 1×10 2 Up to 1×10 7 spores / mL medium, for example, about 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 spores / mL culture medium, etc., but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0061] In conjunction with the third aspect, in some feasible embodiments, in step 1), the inoculation amount of fungal spores is 1×10 2 Up to 1×10 7 spores / g medium, for example, about 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 spores / g culture medium, etc., but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0062] In combination with the third aspect, in some feasible embodiments, in step 1), the culture medium is a sterile culture medium.

[0063] In combination with the third aspect, in some feasible embodiments, in step 1), the culture medium is a liquid culture medium.

[0064] In conjunction with the third aspect, in some feasible embodiments, in step 1), the culture medium contains or consists of the following items: 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH 4 Cl, 0.3g / L to 6g / L KH 2 PO 4 , 0.25g / L to 4g / LMgSO 4 7H 2 0, 0g / L to 100g / LNaCl; Optionally, the glucose is anhydrous glucose.

[0065] In conjunction with the third aspect, in some feasible embodiments, in step 1), the culture medium contains or consists of the following items: 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH 4 Cl, 0.3g / L to 6g / L KH 2 PO 4 , 0.25g / L to 4g / LMgSO 4 7H 2 O, 0g / L to 100g / LNaCl, add water to make up to 1L; optionally, the glucose is anhydrous glucose.

[0066] In conjunction with the third aspect, in some feasible embodiments, in step 2), magnetic powder is added to the fungal suspension during any one of the spore stage, hyphae emergence stage, hyphae aggregation stage, and hyphae ball maturity stage of the fungus;

[0067] In conjunction with the third aspect, in some feasible embodiments, in step 2), the size of the magnetic powder is 3 μm to 100 μm, for example 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52 , 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 μm, etc., but are not limited to the listed values, and other values ​​or ranges not listed within the numerical range are also applicable.

[0068] In combination with the third aspect, in some feasible embodiments, in step 2), the mass of the added magnetic powder accounts for 1% to 50% of the mass of the magnetic powder-enhanced active mycelial ball, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, %, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, and the like, but are not limited to the listed values, and other values ​​or ranges not listed within the numerical range are also applicable.

[0069] In combination with the third aspect, in some feasible embodiments, in step 2), the culture medium is prepared after high-pressure sterilization.

[0070] In combination with the third aspect, in some feasible embodiments, in step 2), the fungal suspension is cultured at 15°C to 35°C, for example, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35°C, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0071] In combination with the third aspect, in some feasible embodiments, in step 2), the culture condition of the fungal suspension is 60 rpm to 400 rpm, for example, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400 rpm, and the like, but is not limited to the listed values, and other values ​​or ranges not listed within the numerical range are equally applicable.

[0072] In combination with the third aspect, in some feasible embodiments, in step 2), the culturing conditions of the fungal suspension are constant temperature culturing for 20 to 240 hours, for example, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240 hours, and the like, but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are equally applicable.

[0073] In combination with the third aspect, in some feasible embodiments, in step 3), the inoculation amount of the magnetic powder-enhanced active mycelium ball is 0.1 g / L to 10 g / L, for example, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 g / L, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within this numerical range are also applicable.

[0074] In combination with the third aspect, in some feasible implementation schemes, in step 3), the magnetic powder-enhanced active mycelium balls and activated sludge are inoculated in a reactor; the mass ratio of the activated sludge to the magnetic powder-enhanced active mycelium balls is 0 to 90:1, for example 0:1, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within this numerical range are equally applicable.

[0075] In combination with the third aspect, in some feasible implementation schemes, in step 4), the sequencing batch operation process includes water inlet, aeration, static sedimentation, water outlet and idle stages.

[0076] In combination with the third aspect, in some feasible implementation schemes, in step 4), the source of wastewater includes at least one of domestic wastewater, municipal wastewater, food industry wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater, printing and dyeing wastewater, animal husbandry wastewater and prepared simulated wastewater.

[0077] In conjunction with the third aspect, in some feasible implementation schemes, in step 4), when wastewater is used as influent, the organic load range of the reaction system is 0.3 kg COD / (m 3 ·day) to 25kg COD / (m 3 ·day), for example 0.3, 0.5, 0.8, 1.2, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 kg COD / (m 3 ·days), etc., but are not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0078] In combination with the third aspect, in some feasible embodiments, the time for forming aerobic granular sludge is 1 to 20 days, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 days, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0079] In combination with the third aspect, in some feasible implementation schemes, in step 4), the total time of each operation cycle of the batch operation is 1 hour to 24 hours, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 hours, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0080] In combination with the third aspect, in some feasible embodiments, in step 4), the pH of the reaction system is maintained at 5 to 9 during batch operation, for example 5, 6, 7, 8, 9, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0081] In combination with the third aspect, in some feasible embodiments, in step 4), the reaction temperature during batch operation is maintained at 15°C to 35°C, for example, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35°C, and the like, but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0082] In combination with the third aspect, in some feasible embodiments, in step 4), the volume exchange rate per cycle during batch operation is maintained at 30% to 80%, for example, 30%, 40%, 50%, 60%, 70%, 80%, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0083] In combination with the third aspect, in some feasible implementation schemes, in step 4), during the aeration stage of sequencing batch operation, air enters from the bottom, and the superficial gas velocity (the ratio of air flow rate to the cross-sectional area in the reactor) is maintained at 0.1 cm / s to 4.5 cm / s, for example, 0.1, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 4.5 cm / s; the dissolved oxygen concentration is controlled at 2 mg / L to 10 mg / L, for example, 2, 3, 4, 5, 6, 7, 8, 9, 10 mg / L, etc., but is not limited to the listed values, and other values ​​or ranges not listed within the numerical range are equally applicable.

[0084] In combination with the third aspect, in some feasible implementation schemes, in step 4), the time range of the static sedimentation stage during batch operation is 0.1 min to 30 min, for example, 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 min, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are equally applicable.

[0085] As used herein, the term "magnetic powder" refers to a hard magnetic single-domain particle, mainly oxide magnetic powder and metal magnetic powder. Oxide magnetic powder includes iron oxide magnetic powder, chromium dioxide magnetic powder and cobalt-iron oxide magnetic powder.

[0086] As used herein, the term "organic load" refers to the amount of organic pollutants accepted by a unit volume of wastewater treatment reactor per unit time, generally excluding organic matter in the reactor reflux (when a reflux system is used). Organic matter can be expressed as COD, so it is also called COD load, with a unit of kg / (m 3 ·day). In the field of sewage treatment, load represents the capacity of sewage treatment facilities to receive sewage. In other words, organic load is an indicator of the treatment capacity of sewage treatment facilities.

[0087] As used herein, the term "aerobic granular sludge (AGS)" refers to a densely structured bioaggregate formed by self-agglomeration of microorganisms in sludge under an aerobic environment. Compared with ordinary activated sludge, aerobic granular sludge has the characteristics of being less prone to sludge swelling, strong impact resistance, able to withstand high organic loads, and integrating microorganisms of different properties (aerobic, facultative anerobic and anaerobic microorganisms).

[0088] As used herein, the term "mycelium ball" refers to a filamentous fungus that is self-entangled and has a large number of mutually entangled hyphae. Therefore, even without adding chemical agents for flocculation, it can gather particulate matter and functional flora in the environment through the capture effect between cells and the electrostatic adsorption effect of surface charges. Mycelium balls have the characteristics of large specific surface area, excellent adsorption performance, fast sedimentation rate, conducive to solid-liquid separation, high biocompatibility, and no secondary pollution.

[0089] The method for determining the particle size of mycelial balls was as follows: 30 mycelial balls were randomly selected, photographed, and the diameters of the mycelial balls were measured using image processing software (ImageJ.JS, v0.5.5). The average value was taken as the average particle size of the mycelial balls.

[0090] As used herein, the term "Sequencing Batch Reactor (SBR)" refers to a reactor that operates intermittently in a time sequence, which is characterized by complete mixing in space and alternating in time, and the entire treatment process (including denitrification and dephosphorization processes) is carried out in the same reactor.

[0091] The aerobic granular sludge in this application shows great potential to replace traditional activated sludge technology: (1) Granular sludge has excellent sedimentation performance, which can save the secondary sedimentation tank with a large footprint and the return facilities with huge energy consumption; (2) Aerobic granular sludge has a dense structure, so this technology has the advantages of high sludge content, strong sludge retention capacity, and strong resistance to stress (such as salinity, toxic substances, water quality fluctuations, etc.); (3) Aerobic granular sludge has a hierarchical structure, with the inner core being the anaerobic / anoxic zone and the outer layer being the aerobic zone, which gives different microorganisms a suitable ecological niche, allowing multiple biological reactions such as biological oxidation, nitrification, denitrification, and phosphorus accumulation to be carried out in the same granule.

[0092] The mycelial pellets in this application have the advantages of fast sedimentation speed, easy solid-liquid separation, high biological activity, strong adsorption capacity for pollutants, and the ability to secrete a variety of enzymes. Adding mycelial pellets to activated sludge can serve as a core carrier to provide space for microorganisms to adsorb and grow, promote the aggregation of microorganisms, and thus accelerate the formation of granular sludge.

[0093] The temperature parameters in this application, unless otherwise specified, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range of instrument control.

[0094] Compared with the prior art, the present invention has the following beneficial effects:

[0095] (1) Compared with the prior art, the magnetic powder used in the present invention does not require a complicated processing and preparation process, the cost is controlled, and the magnetic powder is fixed by active mycelium balls, which significantly reduces the loss of magnetic powder and significantly reduces the cost. It only needs to be added once when the reactor is started to achieve a rapid granulation process;

[0096] (2) Compared with the prior art, the present invention uses magnetic powder-enhanced active mycelium balls, which further enhance the strength of the mycelium balls, avoid the problems of breakage, floating and loss in the initial stage of addition, and can obtain aerobic granular sludge more stably in a shorter time;

[0097] (3) Compared with the prior art, the present invention cultivates specific fungi in a sterile culture medium and then adds them, thereby avoiding the problem of instability of the system in the early stage of granular sludge cultivation; at the same time, the mycelial balls are strengthened with magnetic powder, which further enhances their stability and shortens the time for aerobic granular sludge formation;

[0098] (4) Compared with the prior art, the mycelium balls introduced in the present invention are both active organisms with pollutant degradation functions and carriers for the attachment of magnetic powder and other microorganisms; the magnetic powder can not only promote the agglomeration of flocculent sludge, but also strengthen the strength of the mycelium balls. With the mutual support of mycelium balls and magnetic powder, after the magnetic powder-reinforced active mycelium balls are added to the flocculent sludge system, the promotion effect on the formation of aerobic granular sludge is more obvious than the addition of a single nano-magnet;

[0099] (5) Compared with the prior art, the present invention uses selected fungi that can form balls, such as Aspergillus polydorum and Cladosporium exigua, as fixed carriers of magnetic powder, which is more environmentally friendly and does not require increasing the aeration volume due to the addition of substances; and the magnetic powder-enhanced active mycelium balls obtained by the present invention have better wastewater treatment and promote sludge granulation in the process of granular sludge formation, which helps to give full play to the maximum advantage of granular sludge method in saving energy consumption;

[0100] (6) Compared with the prior art, the present invention uses large-size (3-100 μm) magnetic powder to strengthen the active mycelial balls. On the one hand, the presence of the magnetic powder enhances the growth and metabolic activity of the mycelial balls; on the other hand, the mycelial balls also provide a carrier for the magnetic powder, effectively preventing the waste of magnetic powder (entering the dead corner of the reactor or flowing out of the reactor with the effluent); the cost investment of large-size magnetic powder and mycelial balls is much lower than that of magnetic nanoparticles, and they also have good biological effects. The presence of mycelial balls can provide a skeleton for other microorganisms, which can better improve the sedimentation performance of sludge and significantly accelerate sludge granulation;

[0101] (7) Compared with the prior art, the present invention directly adds magnetic powder-enhanced active mycelium balls with pollutant removal capabilities in the early stage of reactor startup, thereby avoiding the problem of low wastewater removal rate due to the inadaptability of activated sludge in the early stage of reactor startup and ensuring the effluent quality;

[0102] (8) Compared with the prior art, the present invention has lower requirements on the properties of the initial activated sludge, thus avoiding the trouble caused by the different properties of inoculated sludge in different places and improving the controllability of industrial application;

[0103] (9) Compared with the prior art, the present invention can achieve rapid formation of aerobic granular sludge within a wider salinity range (0%-10%). BRIEF DESCRIPTION OF THE DRAWINGS

[0104] Figure 1 The magnetic powder-enhanced active mycelium ball formed in Example 1 is shown;

[0105] Figure 2 shows the aerobic granular sludge formed in Example 1;

[0106] Figure 3 The magnetic powder-enhanced active mycelium pellet formed in Example 2 is shown;

[0107] Figure 4 shows the aerobic granular sludge formed in Example 2;

[0108] Figure 5 The aerobic granular sludge formed in Comparative Example 1 is shown;

[0109] Figure 6 The aerobic granular sludge formed in Comparative Example 3 is shown. DETAILED DESCRIPTION

[0110] In order to better explain the present invention, the embodiments of the present application will be described in detail below in conjunction with specific examples, but those skilled in the art will appreciate that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. The specific conditions not specified in the examples are carried out according to the conditions recommended by normal conditions or manufacturers. The reagents used or the instruments not specified by the manufacturer are all conventional products that can be obtained by commercial purchase.

[0111] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs. It is further understood that terms, such as defined in commonly used dictionaries, are interpreted in accordance with their meanings in the context of the relevant art and are not in an idealized or overly formal sense unless explicitly so defined herein.

[0112] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context.

[0113] Unless otherwise indicated, the use of any and all examples or exemplary language (such as "for example") provided herein is intended only to better illustrate the invention and is not intended to limit the scope of the invention. Unless expressly stated otherwise, no language in the specification should be construed as indicating that any element is essential to the implementation of the invention.

[0114] As used in this application, the term "about" is used to describe and illustrate small changes. When used in conjunction with an event or situation, the term may refer to an example in which the event or situation occurs precisely and an example in which the event or situation occurs very approximately. For example, when used in conjunction with a numerical value, the term may refer to a range of variation of less than or equal to ±10% of the numerical value, such as less than or equal to ±5%, less than or equal to ±4%, less than or equal to ±3%, less than or equal to ±2%, less than or equal to ±1%, less than or equal to ±0.5%, less than or equal to ±0.1%, or less than or equal to ±0.05%. In addition, amounts, ratios, and other numerical values ​​are sometimes presented in the present application in a range format. It should be understood that such a range format is for convenience and simplicity, and should be flexibly understood to include not only numerical values ​​explicitly designated as range limits, but also all individual numerical values ​​or subranges encompassed within the range, as if each numerical value and subrange were explicitly designated.

[0115] The exemplary invention described herein may appropriately lack any one or more element limitations that are not specifically disclosed herein. Therefore, the terms "comprise", "include", "contain", etc. should be understood broadly and non-restrictively. In addition, the terminology used herein is used as a description, not a limitation, and it is unintentional to use these terminology expressions that do not include any equivalent characteristics, but only describe some of their characteristics, but various modifications are possible within the scope of the present invention according to the rights. Therefore, although the present invention has been specifically disclosed by preferred embodiments and optional features, the modifications disclosed herein to embody the changes of the present invention may be recorded by those skilled in the art, and such modifications and changes will be considered to be within the scope of the present invention.

[0116] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0117] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0118] When amount, concentration or other value or parameter is represented by range, preferred range or a series of upper preferred value and lower preferred value, this should be understood as specifically disclosing all ranges formed by any pairing of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 10", described range should be interpreted as including range "1 to 10", "1 to 9", "1 to 8", "1 to 7", "1 to 6", "1 to 5", "1 to 4", "1 to 3", "1 to 2", "1 to 3 and 5 to 10", "1 to 4 and 8" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0119] In the detailed description and claims, a list of items connected by the terms "at least one of," "at least one of," "at least one of," or other similar terms may mean any combination of the listed items. For example, if items A and B are listed, the phrase "at least one of A and B" means only A; only B; or A and B. In another example, if items A, B, and C are listed, the phrase "at least one of A, B, and C" means only A; or only B; only C; A and B (excluding C); A and C (excluding B); B and C (excluding A); or all of A, B, and C. Item A may include a single element or multiple elements. Item B may include a single element or multiple elements. Item C may include a single element or multiple elements.

[0120] In addition, the terms "first", "second", and "third" used in this document are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Thus, a feature defined as "first", "second", and "third" may explicitly or implicitly include at least one of the features. In the description of the embodiments of the present disclosure, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0121] Example 1

[0122] A method for accelerating the formation of aerobic granular sludge by adding magnetic powder to the fungus spore stage to form magnetic powder-enhanced active mycelium balls, comprising the following steps:

[0123] 1) In a clean bench, a suspension of Cladosporium tenuissimum fungi and sterilized magnetic powder were inoculated into a shake flask containing sterile culture medium, where the spore inoculation amount was 10 4 spores / mL, the particle size of the magnetic powder is about 5μm, and the dosage is 0.3g / L; the sterile culture medium consists of 10g / L anhydrous glucose, 1g / L NH4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize to prepare. The Cladosporium tenuissimum used was isolated from the soil and was deposited in the General Microbiology Center of the China Microbiological Collection Administration (CGMCC) on March 4, 2022, with the deposit number CGMCC No: 40141.

[0124] 2) Place the shake flask in a shaker and culture at 28°C and 180 rpm for 120 h to obtain 3.5 g / L magnetic powder-enhanced active mycelial pellets (see Figure 1 ).

[0125] 3) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and 1 g / L of activated sludge from a municipal sewage treatment plant.

[0126] 4) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle stage lasted for 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0127] 5) Aerobic granular sludge was formed on the 4th day, with an average particle size of 2600 μm (see Figure 2 ). The COD removal rate reached over 90% on the first day and over 97% on the fourth day.

[0128] Example 2

[0129] A method for accelerating the formation of aerobic granular sludge by adding magnetic powder after the formation of mycelial balls to form magnetic powder-reinforced active mycelial balls, comprising the following steps:

[0130] 1) In a clean bench, a spore suspension of Cladosporium tenuissimum was inoculated into a shake flask containing sterile culture medium, where the spore inoculation amount was 10 4 spores / mL; the sterile culture medium consists of 10g / L anhydrous glucose, 1g / L NH4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize.

[0131] 2) Place the shake flask in a shaker and culture at 28°C and 180 rpm for 120 h to obtain 3 g / L active mycelial pellets.

[0132] 3) Wash the obtained mycelial pellets with sterile isotonic saline in a clean bench;

[0133] 4) Add the sterilized magnetic powder to the shake flask containing the mycelium pellet obtained in step 3) in a clean bench, and culture at 28°C and 180 rpm for 24 hours to obtain magnetic powder-enhanced mycelium pellets (see Figure 3 ), wherein the particle size of the magnetic powder is 5 μm and the dosage is 0.3 g / L.

[0134] 5) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and then 1 g / L of activated sludge from a municipal sewage treatment plant.

[0135] 6) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0136] 7) Aerobic granular sludge was formed on the third day, with an average particle size of 2400 μm (see Figure 4 ). The COD removal rate reached over 90% on the first day and over 97% on the fifth day.

[0137] Example 3

[0138] A method for accelerating the formation of aerobic granular sludge by adding magnetic powder after the formation of mycelial balls to form magnetic powder-reinforced active mycelial balls, comprising the following steps:

[0139] 1) In a clean bench, a spore suspension of Aspergillus sydowii NXY1 was inoculated into a shake flask containing sterile culture medium, where the spore inoculation amount was 10 5 spores / mL; the sterile culture medium consists of 10g / L anhydrous glucose, 1g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize to prepare. The Aspergillus sydowii used was isolated from shale gas produced water and was deposited in the General Microbiology Center of the China Microbiological Collection Administration (CGMCC) on June 17, 2022, with the deposit number CGMCC No: 40215.

[0140] 2) The shake flask was placed in a shaker and cultured at 30°C and 160 rpm for 120 h to obtain 3.2 g / L active mycelial pellets.

[0141] 3) Wash the obtained mycelial pellets with sterile isotonic saline in a clean bench;

[0142] 4) Add the sterilized magnetic powder to the shake flask containing the mycelial pellet obtained in step 3) in a clean bench, and culture at 30° C. and 160 rpm for 24 h to obtain magnetic powder-enhanced mycelial pellets, wherein the particle size of the magnetic powder is 50 μm and the dosage is 0.6 g / L.

[0143] 5) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and then 1 g / L of activated sludge from a municipal sewage treatment plant.

[0144] 6) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0145] 7) Aerobic granular sludge was formed on the 4th day, with an average particle size of 2800 μm. The COD removal rate reached more than 90% on the 2nd day and more than 97% on the 6th day.

[0146] Comparative Example 1

[0147] 1) A sequencing batch reactor was inoculated with 4 g / L of activated sludge from a municipal sewage treatment plant.

[0148] 2) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours, including influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, aeration lasted for 345 minutes, static sedimentation time was fixed at 3 minutes, effluent lasted for 2 minutes, and idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0149] At the beginning of the operation of the reactor, the sludge floated severely and almost all the original sludge in the system was discharged. Aerobic granular sludge was formed on the 25th day with an average particle size of 400 μm (see Figure 5 ). The COD removal rate reached over 90% on the 12th day and over 97% on the 23rd day.

[0150] The comparative example 1 is substantially the same as the example 1, except that the magnetic powder enhanced active mycelium pellets are not inoculated.

[0151] Comparative Example 2

[0152] 1) A sequencing batch reactor was inoculated with 4 g / L of activated sludge from a municipal sewage treatment plant.

[0153] 2) Add 0.3 g / L of magnetic powder with a particle size of 5 μm into the sequencing batch reactor.

[0154] 3) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours, including influent, aeration, static sedimentation, effluent and idle stages. Including influent for 5 minutes, aeration for 345 minutes, static sedimentation time for 3 minutes, effluent for 2 minutes, and idle for 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0155] 5) Aerobic granular sludge was formed on the 17th day with an average particle size of 600 μm, but no magnetic powder was detected in the reactor on the 15th day, and after the particles were formed, they broke and floated up and were discharged from the system after the 31st day. The COD removal rate reached more than 90% on the 5th day and more than 97% on the 13th day.

[0156] The comparative example 2 is substantially the same as the example 2, except that only magnetic powder is added, and the magnetic powder-enhanced active mycelium pellets are not inoculated.

[0157] Comparative Example 3

[0158] 1) In a clean bench, a spore suspension of Cladosporium tenuissimum was inoculated into a shake flask containing sterile culture medium, where the spore inoculation amount was 10 4 spores / mL; the sterile culture medium consists of 10g / L anhydrous glucose, 1g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize.

[0159] 2) Place the shake flask in a shaker and culture at 28°C and 180 rpm for 120 h to obtain 3 g / L active mycelial pellets.

[0160] 3) The sequencing batch reactor was inoculated with active mycelial pellets at an inoculation amount of 3 g / L, and then inoculated with 1 g / L of activated sludge taken from a municipal sewage treatment plant.

[0161] 4) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle stage lasted for 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0162] 5) On the 5th day, the mycelial balls were broken in large quantities, and on the 10th day, aerobic granular sludge was formed (see Figure 6 ), the average particle size was 900 μm, but the particles broke after the 40th day. The COD removal rate reached more than 90% on the 3rd day and more than 97% on the 13th day.

[0163] The comparative example 3 is substantially the same as the example 2, but the common active mycelium pellets formed by Cladosporium tenuissimum are inoculated instead of the magnetic powder enhanced active mycelium pellets.

[0164] Comparative Example 4

[0165] 1) In a clean bench, a spore suspension of Aspergillus sydowii NXY1 was inoculated into a shake flask containing sterile culture medium, where the spore inoculation amount was 10 5 spores / mL; the sterile culture medium consists of 10g / L anhydrous glucose, 1g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize.

[0166] 2) The shake flask was placed in a shaker and cultured at 30°C and 160 rpm for 120 h to obtain 3.2 g / L active mycelial pellets.

[0167] 3) The sequencing batch reactor was inoculated with active mycelial pellets at an inoculation amount of 3 g / L, and then inoculated with 1 g / L of activated sludge taken from a municipal sewage treatment plant.

[0168] 4) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle stage lasted for 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0169] 5) On the 7th day, the mycelial balls were broken in large quantities, and on the 11th day, aerobic granular sludge was formed with an average particle size of 1300 μm, but the particles began to break after the 33rd day. The COD removal rate reached more than 90% on the 4th day and more than 97% on the 14th day.

[0170] The comparative example 4 is substantially the same as the example 3, except that the common active mycelium pellets formed by Aspergillus sydowii NXY1 were inoculated instead of the magnetic powder enhanced active mycelium pellets.

[0171] Comparative Example 5

[0172] 1) In a clean bench, a spore suspension of Aspergillus niger (commonly available commercially) was inoculated into a shake flask containing sterile culture medium, wherein the spore inoculation amount was 10 6 spores / mL; the sterile culture medium consists of 15g / L anhydrous glucose, 1g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / LNaCl, add deionized water to 1L, and then sterilize.

[0173] 2) The shake flask was placed in a shaker and cultured at 30°C and 150 rpm for 100 h to obtain 3.5 g / L active mycelial pellets.

[0174] 3) Wash the obtained mycelial pellets with sterile isotonic saline in a clean bench;

[0175] 4) Add the sterilized magnetic powder to the shake flask containing the mycelial pellet obtained in step 3) in a clean bench, and culture at 30° C. and 150 rpm for 24 h to obtain magnetic powder-enhanced mycelial pellets, wherein the particle size of the magnetic powder is 5 μm and the dosage is 0.3 g / L.

[0176] 5) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and then 1 g / L of activated sludge from a municipal sewage treatment plant.

[0177] 6) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0178] 7) Aerobic granular sludge was formed on the 6th day with an average particle size of 2300 μm, but it was broken on the 17th day, re-formed on the 22nd day, and broken again on the 43rd day. The COD removal rate reached more than 90% on the 6th day and more than 97% on the 11th day.

[0179] In general, the method of Comparative Example 5 is the same as that of Example 2. In Comparative Example 5, the active mycelium pellets formed by Aspergillus niger are inoculated instead of the active mycelium pellets formed by Cladosporium tenuissimum.

[0180] Comparative Example 6

[0181] 1) In a clean bench, a spore suspension of Aspergillus tubingensis (commonly available commercially) was inoculated into a shake flask containing sterile culture medium, wherein the spore inoculation amount was 10 4 spores / mL; the sterile culture medium consists of 10 g / L anhydrous glucose, 1 g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize.

[0182] 2) The shake flask was placed in a shaker and cultured at 30°C and 220 rpm for 144 h to obtain 3.1 g / L active mycelial pellets.

[0183] 3) Wash the obtained mycelial pellets with sterile isotonic saline in a clean bench;

[0184] 4) Add the sterilized magnetic powder to the shake flask containing the mycelial pellet obtained in step 3) in a clean bench, and culture at 30° C. and 220 rpm for 24 h to obtain magnetic powder-enhanced mycelial pellets, wherein the particle size of the magnetic powder is 5 μm and the dosage is 0.3 g / L.

[0185] 5) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and then 1 g / L of activated sludge from a municipal sewage treatment plant.

[0186] 6) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0187] 7) Aerobic granular sludge was formed on the 7th day with an average particle size of 1900 μm, but the aerobic granular sludge was broken on the 27th day. The COD removal rate reached more than 90% on the 4th day and more than 97% on the 7th day.

[0188] In general, the method of Comparative Example 6 is the same as Example 2. In Comparative Example 6, the active mycelium pellets formed by magnetic powder reinforcement are formed by Aspergillus tubingensis, but not the active mycelium pellets formed by Cladosporium tenuissimum.

[0189] Comparative Example 7

[0190] 1) In a clean bench, a spore suspension of Phanerochaete chrysosporium (commonly available commercially) was inoculated into a shake flask containing sterile culture medium, wherein the spore inoculation amount was 10 5 spores / mL; the sterile culture medium consists of 12 g / L anhydrous glucose, 1.5 g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / LNaCl, add deionized water to 1L, and then sterilize.

[0191] 2) The shake flask was placed in a shaker and cultured at a constant temperature of 25°C and 160 rpm for 120 h to obtain 4.1 g / L active mycelial pellets.

[0192] 3) Wash the obtained mycelial pellets with sterile isotonic saline in a clean bench;

[0193] 4) Add the sterilized magnetic powder to the shake flask containing the mycelial pellet obtained in step 3) in a clean bench, and culture at 25° C. and 160 rpm for 24 hours to obtain magnetic powder-enhanced mycelial pellets, wherein the particle size of the magnetic powder is 5 μm and the dosage is 0.3 g / L.

[0194] 5) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and then 1 g / L of activated sludge from a municipal sewage treatment plant.

[0195] 6) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0196] 7) Aerobic granular sludge was formed on the 13th day with an average particle size of 2200 μm, but the aerobic granular sludge was broken on the 25th day. The COD removal rate reached more than 90% on the 5th day and more than 97% on the 12th day.

[0197] In general, the comparative example 7 is the same as the example 2, but the inoculation is performed with the magnetic powder enhanced active mycelium pellet formed by Phanerochaetechrysosporium, instead of the magnetic powder enhanced active mycelium pellet formed by Cladosporium tenuissimum.

[0198] Comparative Example 8

[0199] 1) In a clean bench, a suspension of Phoma sp. fungi spores (commonly available commercially) was inoculated into a shake flask containing sterile culture medium, wherein the spore inoculation amount was 10 4 spores / mL; the sterile culture medium consists of 20g / L anhydrous glucose, 1g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize.

[0200] 2) The shake flask was placed in a shaker and cultured at 30°C and 200 rpm for 120 h to obtain 5.2 g / L active mycelial pellets.

[0201] 3) Wash the obtained mycelial pellets with sterile isotonic saline in a clean bench;

[0202] 4) Add the sterilized magnetic powder to the shake flask containing the mycelial pellet obtained in step 3) in a clean bench, and culture at 30° C. and 200 rpm for 24 h to obtain magnetic powder-enhanced mycelial pellets, wherein the particle size of the magnetic powder is 5 μm and the dosage is 0.3 g / L.

[0203] 5) The sequencing batch reactor was inoculated with 3 g / L of magnetic powder-enhanced active mycelial pellets and then 1 g / L of activated sludge from a municipal sewage treatment plant.

[0204] 6) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle time was 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0205] 7) Aerobic granular sludge was formed on the 9th day, with an average particle size of 1900μm, but a large number of white hyphae gradually grew on the formed aerobic granular sludge, causing the particles to settle slowly and be discharged from the system. The COD removal rate reached more than 90% on the 6th day and more than 97% on the 14th day, but the effluent was turbid after the 30th day, and the COD removal rate dropped to 80%.

[0206] In general, the method of comparative example 8 is the same as that of example 2. In comparative example 8, the active mycelium pellets formed by Phoma sp. instead of the active mycelium pellets formed by Cladosporium tenuissimum were inoculated.

[0207] Comparative Example 9

[0208] 1) In a clean bench, a spore suspension of Penicillium citrinum (commonly available commercially) and sterilized magnetic powder were inoculated into a shake flask containing sterile culture medium, wherein the spore inoculation amount was 10 5 spores / mL, the particle size of the magnetic powder is 5μm, and the dosage is 0.3g / L; the sterile culture medium consists of 10g / L anhydrous glucose, 1g / L NH 4 Cl, 0.7 g / L KH 2 PO 4 、0.5g / L MgSO 4 7H 2 O and 30g / L NaCl, add deionized water to 1L, and then sterilize.

[0209] 2) The shake flask was placed in a shaker and cultured at 28°C and 180 rpm for 120 h. Flocs with magnetic powder attached were obtained, but no spheres were formed.

[0210] 3) The obtained magnetic powder-attached flocs were inoculated in a sequencing batch reactor at an inoculation amount of 3 g / L and an activated sludge inoculation amount of 1 g / L.

[0211] 4) 3% salinity pickled food wastewater was added as influent to the sequencing batch reactor. Each operation cycle lasted for 6 hours. One cycle included influent, aeration, static sedimentation, effluent and idle stages. The influent lasted for 5 minutes, the aeration lasted for 345 minutes, the static sedimentation time was fixed at 3 minutes, the effluent lasted for 2 minutes, and the idle stage lasted for 5 minutes. The volume exchange rate of each cycle was maintained at 60%, and the organic load of the reaction system was 2.4 kg COD / (m 3 ·days). During operation, the pH of the reaction system was maintained at 6.5-7.5, the temperature was maintained at 25±3°C, and air entered from the bottom during the aeration phase, with the superficial gas velocity maintained at 1.2 cm / s.

[0212] 5) The flocs attached by magnetic powder were discharged from the reactor in large quantities on the 5th day, and aerobic granular sludge was not formed within 60 days. The COD removal rate reached more than 90% on the 22nd day, but did not reach more than 97% within 60 days.

[0213] In general, the method of Comparative Example 9 is the same as that of Example 2. In Comparative Example 9, the active mycelium pellets formed by Penicillium citrinum instead of the active mycelium pellets formed by Cladosporium tenuissimum were inoculated.

[0214] Table 1: Effects of various embodiments and comparative examples.

[0215]

[0216] The above description is only a specific embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent transformation made by the present invention, or directly or indirectly applied in other related technical fields, is also included in the patent protection scope of the present invention.

[0217] For the specific conditions not specified in the examples, the conventional conditions or the conditions recommended by the manufacturer were followed. For the reagents or instruments used without specifying the manufacturer, they were all conventional products that can be purchased commercially.

Claims

1. Aerobic granular sludge cultured with magnetic powder-enhanced active mycelium balls, characterized in that: The aerobic granular sludge is prepared by a method comprising the following steps: 1) Inoculating fungal spores in a culture medium, culturing and obtaining a fungal suspension; 2) adding magnetic powder to the fungal suspension for co-cultivation to obtain magnetic powder-enhanced active mycelium pellets; 3) inoculating the magnetic powder enhanced active mycelium pellets in a reactor; 4) operating the reactor in a batch mode to treat wastewater, and cultivating and obtaining the aerobic granular sludge; The fungus comprises at least one of Aspergillus polydorum and Cladosporium exigua, and the name of Aspergillus polydorum is Aspergillus polydorum NXY1 ( Aspergillus sydowii NXY1), with a deposit number of CGMCC No: 40215; the name of the Cladosporium exigua is Cladosporium exigua NXY8 ( Cladosporium tenuissimum NXY8), the deposit number is CGMCC No:40141.

2. The aerobic granular sludge according to claim 1, characterized in that: The aerobic granular sludge satisfies at least one of the following conditions a to r: a. In step 1), the inoculum amount of the fungal spores is 1×10 2 Up to 1×10 7 spores / mL medium; b. In step 1), the inoculum amount of the fungal spores is 1×10 2 Up to 1×10 7 spores / g medium; c. In step 1), the culture medium is a sterile culture medium; d. In step 1), the culture medium is a liquid culture medium; e. In step 1), the culture medium contains 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH4Cl, 0.3 g / L to 6 g / L KH2PO4, 0.25 g / L to 4 g / L MgSO4·7H2O, and 0 g / L to 100 g / L NaCl; f. In step 1), the culture medium contains 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH4Cl, 0.3 g / L to 6 g / L KH2PO4, 0.25 g / L to 4 g / L MgSO4·7H2O, 0 g / L to 100 g / L NaCl, and water is added to make up to 1 L; g. In step 2), magnetic powder is added to the fungal suspension during any one of the spore stage, hyphae emergence stage, hyphae aggregation stage, and mycelial ball maturity stage of the fungus; h. In step 2), the size of the magnetic powder is 3 µm to 100 µm; i. In step 2), the mass of the magnetic powder added accounts for 1% to 50% of the mass of the magnetic powder-enhanced active mycelial ball; j. In step 2), the fungal suspension is cultured at 15°C to 35°C; k. In step 2), the fungal suspension is cultured at 60 rpm to 400 rpm; l. Step 2), the fungal suspension is cultured at a constant temperature for 20 h to 240 h; m. In step 3), the inoculation amount of the magnetic powder-enhanced active mycelium ball is 0.1 g / L to 10 g / L; n. In step 3), the magnetic powder enhanced active mycelium pellets and activated sludge are inoculated in a reactor; the mass ratio of the activated sludge to the magnetic powder enhanced active mycelium pellets is 0 to 90:1; o. In step 4), the batch operation process includes water inlet, aeration, static sedimentation, water outlet and idle stage; p. In step 4), the source of the wastewater includes at least one of domestic wastewater, municipal wastewater, food industry wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater, printing and dyeing wastewater, animal husbandry wastewater and prepared simulated wastewater; q. In step 4), when wastewater is used as influent, the organic load range of the reaction system is 0.3 kg COD / (m 3 ·day) to 25kg COD / (m 3 ·sky); r. The aerobic granular sludge is formed over a period of 1 to 20 days.

3. The aerobic granular sludge according to any one of claims 1 to 2, characterized in that: In step 4), the batch operation satisfies at least one of the following conditions a to f: a. The total time of each operation cycle of batch operation is 1 h to 24 h; b. The pH of the reaction system is maintained at 5 to 9 during batch operation; c. The reaction temperature during batch operation is maintained at 15°C to 35°C; d. The volume exchange rate per cycle during batch operation is maintained at 30% to 80%; e. During the aeration phase of sequencing batch operation, air enters from the bottom, the superficial air velocity is maintained at 0.1 cm / s to 4.5 cm / s, and the dissolved oxygen concentration is controlled at 2 mg / L to 10 mg / L; f. The time range for the static settling phase in batch operation is 0.1 min to 30 min.

4. Use of aerobic granular sludge cultured with magnetic powder-enhanced active mycelial pellets according to any one of claims 1 to 3 in wastewater treatment.

5. The use according to claim 4, characterized in that The source of the wastewater includes at least one of domestic wastewater, municipal wastewater, food industry wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater, printing and dyeing wastewater, animal husbandry wastewater, and prepared simulated wastewater.

6. A method for accelerating the formation of aerobic granular sludge using magnetic powder-enhanced active mycelium pellets, characterized in that: The method comprises the following steps: 1) Inoculating fungal spores in a culture medium, culturing and obtaining a fungal suspension; 2) adding magnetic powder to the fungal suspension for co-cultivation to obtain magnetic powder-enhanced active mycelium pellets; 3) inoculating the magnetic powder enhanced active mycelium pellets in the reactor; 4) operating the reactor in a batch mode to treat wastewater, and cultivating and obtaining the aerobic granular sludge; The fungus comprises at least one of Aspergillus polydorum and Cladosporium exigua, and the name of Aspergillus polydorum is Aspergillus polydorum NXY1 ( Aspergillus sydowii NXY1), with a deposit number of CGMCC No: 40215; the name of the Cladosporium exigua is Cladosporium exigua NXY8 ( Cladosporium tenuissimum NXY8), the deposit number is CGMCC No:40141.

7. The method according to claim 6, characterized in that The method satisfies at least one of the following conditions a to r: a. In step 1), the inoculum amount of the fungal spores is 1×10 2 Up to 1×10 7 spores / mL medium; b. In step 1), the inoculum amount of the fungal spores is 1×10 2 Up to 1×10 7 spores / g medium; c. In step 1), the culture medium is a sterile culture medium; d. In step 1), the culture medium is a liquid culture medium; e. In step 1), the culture medium contains 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH4Cl, 0.3 g / L to 6 g / L KH2PO4, 0.25 g / L to 4 g / L MgSO4·7H2O, and 0 g / L to 100 g / L NaCl; f. In step 1), the culture medium contains 5 g / L to 80 g / L glucose, 0.5 g / L to 8 g / L NH4Cl, 0.3 g / L to 6 g / L KH2PO4, 0.25 g / L to 4 g / L MgSO4·7H2O, 0 g / L to 100 g / L NaCl, and water is added to make up to 1 L; g. In step 2), magnetic powder is added to the fungal suspension during any one of the spore stage, hyphae emergence stage, hyphae aggregation stage, and mycelial ball maturity stage of the fungus; h. In step 2), the size of the magnetic powder is 3 µm to 100 µm; i. In step 2), the mass of the magnetic powder added accounts for 1% to 50% of the mass of the magnetic powder-enhanced active mycelial ball; j. In step 2), the fungal suspension is cultured at 15°C to 35°C; k. In step 2), the fungal suspension is cultured at 60 rpm to 400 rpm; l. Step 2), the fungal suspension is cultured at a constant temperature for 20 h to 240 h; m. In step 3), the inoculation amount of the magnetic powder-enhanced active mycelium ball is 0.1 g / L to 10 g / L; n. In step 3), the magnetic powder enhanced active mycelium pellets and activated sludge are inoculated in a reactor; the mass ratio of the activated sludge to the magnetic powder enhanced active mycelium pellets is 0 to 90:1; o. In step 4), the batch operation process includes water inlet, aeration, static sedimentation, water outlet and idle stage; p. In step 4), the source of the wastewater includes at least one of domestic wastewater, municipal wastewater, food industry wastewater, oil and gas field wastewater, chemical industry wastewater, light industry wastewater, printing and dyeing wastewater, animal husbandry wastewater and prepared simulated wastewater; q. In step 4), when wastewater is used as influent, the organic load range of the reaction system is 0.3 kg COD / (m 3 ·day) to 25kg COD / (m 3 ·sky); r. The aerobic granular sludge is formed over a period of 1 to 20 days.

8. The method according to claim 6, characterized in that In step 4), the batch operation satisfies at least one of the following conditions a to f: a. The total time of each operation cycle of batch operation is 1 h to 24 h; b. The pH of the reaction system is maintained at 5 to 9 during batch operation; c. The reaction temperature during batch operation is maintained at 15°C to 35°C; d. The volume exchange rate per cycle during batch operation is maintained at 30% to 80%; e. During the aeration phase of sequencing batch operation, air enters from the bottom, the superficial air velocity is maintained at 0.1 cm / s to 4.5 cm / s, and the dissolved oxygen concentration is controlled at 2 mg / L to 10 mg / L; f. The time range for the static settling phase in batch operation is 0.1 min to 30 min.

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