A pesticide composition for preventing and treating passion fruit virus disease

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for preventing and treating passion fruit virus disease. A pesticide composition for preventing and treating passion fruit virus disease, wherein the active ingredients of the pesticide composition are compounded with sitosterol and chitosan, glucan or pyraclostrobin. When the active ingredients in the pesticide composition of the present invention are compounded in a certain mass ratio, they have a synergistic effect on the pathogen of passion fruit virus disease, can improve the prevention and treatment effect of passion fruit virus disease, can reduce the use amount of the active ingredients, and reduce the prevention and treatment cost.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating passion fruit virus disease. Background Art

[0002] Passion fruit virus disease is the most common disease in passion fruit cultivation, also known as mosaic disease. Virus disease can cause a decrease in the yield and quality of passion fruit, posing a serious threat to the development of the passion fruit industry and becoming one of the main factors restricting the development of the industry.

[0003] At present, more than 20 types of viruses that cause passion fruit virus disease have been identified, among which cucumber mosaic virus CMV, passion fruit lignification virus PWV, East Asian passion fruit virus EAPV and tuberose mosaic virus TeMV are the most widely distributed. Among them, cucumber mosaic virus CMV is the most common and most harmful pathogen in viral diseases.

[0004] Sitosterol, also known as phytosterol, β-sitosterol, CAS: 83-46-5, the structural formula is as follows:

[0005]

[0006] Studies have shown that sitosterol can be used to prevent and treat crop viral diseases. Although sitosterol alone has a certain preventive effect on crop viral diseases, single-ingredient agents are prone to cause viral resistance, that is, there is a risk of resistance when sitosterol is used alone.

[0007] Application No. CN201310488412.X discloses an antiviral composition containing carotene and sitosterol and its antiviral application, and specifically discloses that the antiviral composition contains active ingredients carotene and sitosterol, and the weight ratio of the two is 1:50 to 50:1. Compared with the use of carotene or sitosterol alone, the antiviral composition disclosed in the invention has a significant synergistic effect on a variety of plant viruses; at the same time, it can not only prolong the service life of the active compound, but also delay the generation of plant virus resistance.

[0008] The inventors found through indoor experiments that when sitosterol is compounded with chitosan, glucan or pyraclostrobin, it has a synergistic effect on cucumber mosaic virus CMV that causes passion fruit virus disease, which can not only improve the prevention and control effect of viral diseases, but also reduce the risk of drug resistance when sitosterol is used alone.

[0009] At present, there are no reports on the combination of sitosterol with chitosan, glucan or pyraclostrobin.

[0010] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0011] The object of the present invention is to provide a pesticide composition for preventing and treating passion fruit virus disease, so as to solve the problem of pesticide resistance existing in the use of sitosterol alone.

[0012] To achieve the above object, the present invention provides the following technical solutions:

[0013] A pesticide composition, the active ingredients of which are a combination of sitosterol and chitosan, glucan or pyraclostrobin.

[0014] Preferably, the mass ratio of sitosterol to chitosan is 1-9:7-1.

[0015] Preferably, the mass ratio of sitosterol to glucan is 1-20:15-1.

[0016] Preferably, the mass ratio of sitosterol to pyraclostrobin is 1-40:10-1.

[0017] In addition, the present invention also provides application of the pesticide composition in preventing and treating passion fruit virus disease.

[0018] Preferably, the pathogen of the passion fruit virus disease is cucumber mosaic virus (CMV).

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) When the active ingredients in the pesticide composition of the present invention are compounded in a certain mass ratio, they have a synergistic effect on the pathogen of passion fruit virus disease. Compared with a single active ingredient, the control effect of passion fruit virus disease can be improved, the usage amount of the active ingredient can be reduced, and the control cost can be reduced.

[0021] (2) The pesticide composition of the present invention can delay the development of drug resistance in plant viruses and reduce the risk of drug resistance when using a single active ingredient. DETAILED DESCRIPTION

[0022] The following is a clear and complete description of the technical solution of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example :Indoor biological activity test

[0024] 1. Test virus source: Collect fresh leaves infected with cucumber mosaic virus CMV in the "Zixiang No. 1" passion fruit planting area, add PBS buffer (0.01mol / L, pH7.2), and then add a little 500-mesh quartz sand, grind the homogenate, filter the residue with 4 layers of gauze to obtain the virus liquid, and set aside. Among them, the ratio of fresh leaf mass to PBS buffer volume is 50mg:1L.

[0025] 2. Test reagents

[0026] 95% Sitosterol technical, 99% Chitosan technical, 95% Glucuron technical and 98% Pyraclostrobin technical are all commercially available.

[0027] After the original drug is dissolved, it is diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple groups of proportions are set, and each single dose and each group of mixed doses are set with 7 gradient mass concentrations according to the equal ratio method. All drugs are prepared and used immediately.

[0028] 3. Test methods

[0029] Select "Zixiang No. 1" passion fruit seedlings with uniform and healthy growth, trim the old leaves, and leave 5 leaves per plant for inoculation. Spray a layer of corundum on the surface of each leaf, and use a brush dipped in the virus solution to gently rub the entire leaf for friction inoculation. After inoculation, gently rinse the leaf surface with clean water, spray the solution after drying, and spray once every 7 days for a total of 3 times. During this period, place it in a light incubator with a temperature of (25±1)℃ for cultivation. Treat 30 seedlings with each solution, repeat 3 times, and set the spraying of clean water as a blank control. Investigate the disease before the first spraying and 7 days after the last spraying, and calculate the disease index and relative prevention effect.

[0030]

[0031]

[0032] 4. Disease progression table

[0033] Table 1 Disease progression table

[0034] Disease level Condition 0 Disease-free 1 A few chlorotic spots or bright veins on the leaves 3 Middle and upper leaves with mosaic 5 The middle and upper leaves are heavily variegated, and a few leaves are wrinkled or deformed 7 Most leaves are heavily mosaic, some leaves are wrinkled, deformed or have necrotic spots, and the plant is slightly dwarfed 9 Most leaves are severely mosaic, deformed or have necrotic spots, and the plants are obviously dwarfed or even die.

[0035] 5. Data analysis: Use DPS software to perform regression analysis on the logarithmic values ​​of each treatment agent concentration and the relative efficacy probability value of each treatment, and calculate the EC of each treatment agent. 50 , and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.

[0036] 6. Efficacy evaluation

[0037] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 2-4.

[0038] Table 2 Results of indoor activity test on sitosterol and chitosan complexes against cucumber mosaic virus CMV

[0039]

[0040]

[0041] As shown in Table 2, the mass ratio of sitosterol to chitosan in the range of 1-9:7-1 showed a synergistic effect on cucumber mosaic virus CMV.

[0042] Table 3 Results of indoor activity test on sitosterol and glucan complex against cucumber mosaic virus CMV

[0043] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Sitosterol 4.0364 100.0000 -- -- Glucan 6.5233 61.8767 -- -- Sitosterol 1:Glucan 15 7.0555 57.2093 64.2594 89.0287 Sitosterol 1:Glucan 10 6.8011 59.3492 65.3424 90.8280 Sitosterol 1:Glucan 5 4.6129 87.5024 68.2305 128.2453 Sitosterol 1:Glucan 3 3.9910 101.1376 71.4075 141.6344 Sitosterol 1:Glucan 1 3.2271 125.0782 80.9383 154.5352 Sitosterol 3:Glucan 1 2.2814 176.9264 90.4692 195.5655 Sitosterol 5:Glucan 1 1.9422 207.8262 93.6461 221.9272 Sitosterol 10:Glucan 1 3.4390 117.3713 96.5342 121.5852 Sitosterol 15:Glucan 1 4.0595 99.4310 97.6173 101.8579 Sitosterol 20:Glucan 1 4.7721 84.5833 98.1846 86.1472

[0044] As shown in Table 3, when sitosterol and glucan are compounded, within the mass ratio range of 1:15-10 and 15-20:1, they exhibit an additive effect on cucumber mosaic virus CMV; within the mass ratio range of 1-10:5-1, they exhibit a synergistic effect on cucumber mosaic virus CMV.

[0045] Table 4 Indoor activity test results of sitosterol and pyraclostrobin against cucumber mosaic virus CMV

[0046] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Sitosterol 4.0364 100.0000 -- -- Pyraclostrobin 2.5917 155.7433 -- -- Sitosterol 1: Pyraclostrobin 10 2.4183 166.9106 150.6758 110.7747 Sitosterol 1: Pyraclostrobin 5 1.7963 224.7063 146.4528 153.4326 Sitosterol 1: Pyraclostrobin 3 0.9508 424.5267 141.8075 299.3683 Sitosterol 1: Pyraclostrobin 1 2.1204 190.3603 127.8717 148.8682 Sitosterol 3: Pyraclostrobin 1 2.0801 194.0484 113.9358 170.3137 Sitosterol 5: Pyraclostrobin 1 3.0001 134.5422 109.2906 123.1050 Sitosterol 10: Pyraclostrobin 1 2.5999 155.2521 105.0676 147.7641 Sitosterol 15: Pyraclostrobin 1 3.1154 129.5628 103.4840 125.2009 Sitosterol 20: Pyraclostrobin 1 1.3914 290.0963 102.6544 282.5950 Sitosterol 30: Pyraclostrobin 1 0.9662 417.7603 101.7982 410.3809 Sitosterol 40: Pyraclostrobin 1 0.8852 455.9873 101.3596 449.8709

[0047] As shown in Table 4, the mass ratio of sitosterol to pyraclostrobin in the range of 1-40:5-1 showed a synergistic effect on cucumber mosaic virus CMV.

[0048] In summary, when sitosterol is compounded with chitosan, glucan or pyraclostrobin, it has a synergistic effect on cucumber mosaic virus CMV of passion fruit virus disease. Compared with a single active ingredient, it can improve the prevention and control effect of passion fruit virus disease, reduce the use of active ingredients, and reduce the cost of prevention and control.

[0049] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A pesticide composition for preventing and treating passion fruit virus disease, wherein the pathogen of the passion fruit virus disease is cucumber mosaic virus (CMV), characterized in that: The effective component is prepared by compounding sitosterol and pyraclostrobin, and the mass ratio of the sitosterol to pyraclostrobin is 1-40:5-1.

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