A kind of smear ointment for promoting microcirculation and preparation method thereof

Abstract

The present application proposes a smear cream for promoting microcirculation and a preparation method thereof, wherein the smear cream includes the following raw materials in parts by weight: plant extract, 15-30 parts of oil phase, and 5-15 parts of emulsifier; the raw materials of the plant extract include: 5-12 parts of safflower, 4-8 parts of prickly ash, 5-9 parts of cyperus, 3-6 parts of lithospermum, 3-8 parts of salvia miltiorrhiza, and 1-3 parts of coix seed. The present application compounds a variety of plant extracts, finds the best plant extract composition for promoting microcirculation through experiments, and makes it into a smear cream, which is convenient to use and easy to apply, while making the release of plant extract components and better intradermal penetration effect.

Description

Technical Field

[0001] The present application belongs to the field of microbial fermentation, and specifically relates to an ointment for promoting microcirculation and a preparation method thereof. Background Art

[0002] The flow of blood, lymph and tissue fluid that directly participates in the transfer of substances, information and energy between tissues and cells is called microcirculation. Microcirculation is a system that participates in the exchange of substances, energy and biological information between cells and tissues. Skin microcirculation is composed of two parallel vascular plexuses, deep and shallow, which play the role of storing blood, nourishing the skin and maintaining the stability of skin microcirculation. As an important part of the skin barrier, changes in skin blood flow are affected by physiological, pathological and environmental factors. Different skin conditions and topical drugs can cause changes in skin blood flow and change skin microcirculation. Improving skin microcirculation can enhance the activity of skin cells, provide skin cells with sufficient nutrients and nutrients, remove cell metabolites and various harmful substances, reduce the oxidation of collagen fibers, and thus delay skin aging.

[0003] CN115006318B discloses a multi-functional skin care product for softening water molecules, and CN113975217B discloses a whitening and anti-aging composition based on herbal plants, both of which are compounded with plant extracts, peptides and other ingredients to improve skin microcirculation.

[0004] After actual use, the technical solution of the above existing patent is not optimistic about the effect of promoting and improving microcirculation. Summary of the invention

[0005] In order to solve the above problems, the present application proposes a smear cream for promoting microcirculation, comprising the following raw materials in parts by weight: plant extract, 15-30 parts of oil phase, and 5-15 parts of emulsifier;

[0006] The raw materials of the plant extract include: 5-12 parts of safflower, 4-8 parts of prickly ash, 5-9 parts of cyperus rotundus, 3-6 parts of lithospermum officinale, 3-8 parts of salvia miltiorrhiza and 1-3 parts of coix seed.

[0007] Furthermore, the oil phase is one or more of white vaseline, stearyl alcohol, and liquid paraffin.

[0008] Furthermore, the emulsifier is one or more of sodium lauryl sulfate, Span-80, and sodium stearate.

[0009] Furthermore, a microcirculation-promoting ointment is composed of the following raw materials in parts by weight: 9 parts of safflower, 4 parts of Zanthoxylum bungeanum, 6 parts of Vaccinium myrtillus, 4 parts of Lithospermum officinale, 3 parts of Salvia miltiorrhiza, 2 parts of Coix seeds, 10 parts of white vaseline, 8 parts of stearyl alcohol, and 10 parts of sodium stearate.

[0010] Furthermore, it also includes one or more of the following raw materials in parts by weight: 5-8 parts of moisturizer, 0.01-0.1 parts of preservative, 0.2-1 parts of antioxidant, and 0.1-0.3 parts of thickener.

[0011] Furthermore, the moisturizing agent is one or more of propylene glycol, glycerin and urea.

[0012] Furthermore, the preservative is one or more of ethyl paraben and salicylic acid.

[0013] Furthermore, the antioxidant is one or more of vitamin C and sodium sulfite.

[0014] Furthermore, the thickener is one or more of behenyl alcohol, xanthan gum, and hydroxyethyl cellulose.

[0015] Furthermore, in order to make the product more diversified, the functions can be adjusted according to the actual user population, and to better achieve the effect of promoting microcirculation, the present application also includes one or more of the following raw materials in parts by weight: 0.1-0.6 parts of thermal sensation agent, 1-2 parts of L-carnitine, 0.02-0.06 parts of borneol, 0.02-0.06 parts of camphor, and 0.01-0.1 parts of marine extract.

[0016] Furthermore, the thermal agent is one or more of vanillyl butyl ether and capsaicin. Using a proper amount of the thermal agent can produce a sense of heat locally on the skin and promote blood circulation.

[0017] Furthermore, the marine extract is one or more of Undaria pinnatifida extract and Coccophora ovata extract.

[0018] The present application provides an ointment for promoting microcirculation, which is composed of the following raw materials in parts by weight: 10 parts of safflower, 6 parts of Zanthoxylum bungeanum, 6 parts of Vaccinium myrtillus, 3 parts of Lithospermum officinale, 3 parts of Salvia miltiorrhiza, 2 parts of Coix seeds, 15 parts of white vaseline, 12 parts of sodium stearate, 4 parts of glycerin, 2 parts of urea, and 0.05 parts of ethyl parahydroxybenzoate.

[0019] Furthermore, the preparation method of the plant extract includes: after crushing the plant raw material, adding deionized water to the raw material at a material-liquid ratio of 1: (20-40), stirring and soaking for 30-120 minutes, placing in a 50-60°C water bath for 2-3 hours, and then filtering to obtain the plant extract.

[0020] Further, the plant extract is subjected to fermentation treatment, and the fermentation treatment method is as follows: after adding a carbon source and a nitrogen source to the plant extract, the composite fermentation strain is added after sterilization, and the mixture is cultured at 25-30° C. and 150-200 r / min for 48 hours, sterilized, cooled to room temperature, and centrifuged to obtain a supernatant;

[0021] The composite fermentation bacteria are yeast, monascus and lactobacillus plantarum.

[0022] Furthermore, the yeast is one or more of Saccharomyces cerevisiae, Candida, and Trichosporon.

[0023] Furthermore, the inoculation amount of the composite fermentation bacteria is 2-8%;

[0024] The inoculation ratio of the yeast, Monascus purpureus and Lactobacillus plantarum is 1:(0.1-0.3):(0.1-0.4).

[0025] The strains used in this application are counted in terms of viable bacteria, and the yeast concentration is 2*10 5 -9*10 5 CFU / ml, Monascus concentration is 1*10 8 -3*10 8 CFU / ml, Lactobacillus plantarum 3*10 8 -6*10 8 CFU / ml.

[0026] Furthermore, the carbon source is one or more of glucose, fructose, maltose and sucrose, and the added amount of the carbon source is 2-4% of the plant extract.

[0027] Furthermore, the nitrogen source is one or more of casein, trypsin, and ammonium chloride, and the added amount of the nitrogen source is 2-4% of the plant extract.

[0028] The present application also provides a method for preparing a microcirculation-promoting ointment, comprising the following steps:

[0029] (1) Heat and melt the oil phase and other substances that are not easily soluble in water, and stir them evenly;

[0030] (2) Heat the plant extract and the water-soluble substance to dissolve, add 50 ml of water and stir well;

[0031] (3) Slowly add the solution obtained in step (2) to the liquid obtained in step (1), and stir until condensed to obtain a coating paste.

[0032] This application can bring the following beneficial effects:

[0033] 1. The present application compounds a variety of plant extracts, finds the best plant extract composition for promoting microcirculation through experiments, and makes it into an smear, which is convenient to use and easy to apply, while making the release of plant extract ingredients and intradermal penetration better.

[0034] 2. Safflower has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and also has a moisturizing effect on the skin, and its penetration is relatively strong; Sichuan pepper has the effects of antibacterial, dispelling cold and relieving pain; Wangbuliu has the effects of dispersing wind and dampness, opening the orifices and relieving pain, and antibacterial; Lithospermum officinale has the effects of clearing away heat and detoxification, promoting blood circulation and reducing swelling, and removing rashes and spots; Salvia miltiorrhiza has the effects of promoting blood circulation and relieving pain, removing blood stasis and relieving pain; Coix seed has the effects of anti-inflammatory, pus discharge, and anti-rough skin. Multiple plant extracts are compounded to achieve a very good compatibility effect.

[0035] 3. The present application ferments the plant extracts and utilizes microorganisms to carry out biotransformation of the plant raw materials, which can improve the extraction rate of the effective ingredients on the one hand, and on the other hand, convert the obtained plant extracts into small molecules, which have a better intradermal penetration effect.

[0036] 4. This application can add appropriate additives according to product requirements and maintain good stability, making the product ingredients more flexible and diverse.

[0037] 5. The present application first obtains the plant extract, then ferments the plant extract, and selects suitable composite fermentation bacteria, carbon source and nitrogen source to effectively improve the fermentation effect. DETAILED DESCRIPTION

[0038] The raw materials and equipment used in the examples and comparative examples are all conventional commercially available products.

[0039] The preparation method of a microcirculation promoting ointment involved in all embodiments and comparative examples of the present application uses the following steps:

[0040] (1) Heat and melt the oil phase and other substances that are not easily soluble in water, and stir them evenly;

[0041] (2) Heat the plant extract and the water-soluble substance to dissolve, add 50 ml of water and stir well;

[0042] (3) Slowly add the solution obtained in step (2) to the liquid obtained in step (1), and stir until condensed to obtain a coating paste.

[0043] [Example 1]

[0044] A smear for promoting microcirculation is composed of the following raw materials in parts by weight: 5 parts of safflower, 4 parts of prickly ash, 5 parts of Vaccinium myrtillus, 3 parts of lithospermum officinale, 8 parts of salvia miltiorrhiza, 1 part of coix seed, 15 parts of white vaseline and 5 parts of sodium lauryl sulfate.

[0045] The plant extract preparation method is as follows:

[0046] The plant raw materials were mixed together, crushed, and deionized water was added at a solid-liquid ratio of 1:20. After stirring and soaking for 30 minutes, the mixture was placed in a 50° C. water bath for 2 hours and filtered to obtain the plant extract.

[0047] [Example 2]

[0048] A smear for promoting microcirculation is composed of the following raw materials in parts by weight: 12 parts of safflower, 8 parts of prickly ash, 9 parts of cyperus rotundus, 6 parts of lithospermum officinale, 3 parts of salvia miltiorrhiza, 3 parts of coix seeds, 30 parts of liquid paraffin and 15 parts of Span-80.

[0049] The plant extract preparation method is as follows:

[0050] The plant raw materials were mixed together, crushed, and deionized water was added at a solid-liquid ratio of 1:40. After stirring and soaking for 120 minutes, the mixture was placed in a 60° C. water bath for 3 hours and filtered to obtain the plant extract.

[0051] [Example 3]

[0052] A smear for promoting microcirculation is composed of the following raw materials in parts by weight: 9 parts of safflower, 4 parts of prickly ash, 6 parts of Vaccinium myrtillus, 4 parts of lithospermum officinale, 3 parts of salvia miltiorrhiza, 2 parts of coix seeds, 10 parts of white vaseline, 8 parts of octadecyl alcohol and 10 parts of sodium stearate.

[0053] The plant extract preparation method is as follows:

[0054] The plant raw materials were mixed together, crushed, and deionized water was added at a solid-liquid ratio of 1:30. After stirring and soaking for 60 minutes, the mixture was placed in a 55° C. water bath for 2 hours and filtered to obtain the plant extract.

[0055] [Example 4]

[0056] A smear for promoting microcirculation is composed of the following raw materials in parts by weight: 10 parts of safflower, 6 parts of pepper, 6 parts of Vaccinium myrtillus, 3 parts of lithospermum officinale, 3 parts of salvia miltiorrhiza, 2 parts of coix seeds, 15 parts of white vaseline, 12 parts of sodium stearate, 4 parts of glycerol, 2 parts of urea and 0.05 parts of ethyl p-hydroxybenzoate.

[0057] The method for preparing the plant extract is the same as that in Example 3 and is omitted here.

[0058] [Example 5]

[0059] 8 parts of safflower, 4 parts of Zanthoxylum bungeanum, 6 parts of Vaccinium myrtillus, 3 parts of Lithospermum officinale, 4 parts of Salvia miltiorrhiza, 1.5 parts of Coix seeds, 16 parts of octadecyl alcohol, 8 parts of sodium lauryl sulfate, 0.1 parts of salicylic acid, 0.3 parts of vitamin C, and 0.1 parts of xanthan gum.

[0060] The method for preparing the plant extract is the same as that in Example 3 and is omitted here.

[0061] [Example 6]

[0062] The raw materials of this embodiment are the same as those of Embodiment 3, except that the plant extract is fermented again to obtain the fermented plant extract for preparing the smear cream; step (2) in the preparation method of the smear cream is also modified accordingly: the fermented plant extract and the water-soluble substance are heated to dissolve, and 50 ml of water is added and stirred evenly.

[0063] The fermentation method of the plant extract of this embodiment is as follows: 2 wt% glucose and 2 wt% tryptone are added to the plant extract, and 2% of the composite fermentation bacteria are inoculated after sterilization, and cultured at 25°C and 150 r / min for 48 hours, sterilized, cooled to room temperature, and centrifuged to obtain a supernatant;

[0064] The composite fermentation bacteria are yeast, Monascus, and Lactobacillus plantarum, with a mass ratio of 1:0.1:0.1.

[0065] The yeast used in this example is commercially available Saccharomyces cerevisiae CICC30198.

[0066] The Monascus used in this example is commercially available Monascus sp. CICC 40712.

[0067] The plant lactobacillus used in this example is commercially available plant lactobacillus Lactobacillus plantarum CICC21804.

[0068] [Example 7]

[0069] The difference between this embodiment and embodiment 6 is that:

[0070] The fermentation method of the plant extract of this embodiment is as follows: 1wt% fructose, 3wt% maltose, 2wt% casein, and 2wt% ammonium chloride are added to the plant extract, and 8% of the composite fermentation strain is inoculated after sterilization, and cultured at 30°C and 200r / min for 48h, sterilized, cooled to room temperature, and centrifuged to obtain a supernatant;

[0071] The composite fermentation bacteria are yeast, Monascus, and Lactobacillus plantarum, with a mass ratio of 1:0.3:0.4.

[0072] The yeast used in this example is the commercially available Candida utilis CICC 31170.

[0073] The Monascus used in this example is commercially available Monascus sp. CICC 40712.

[0074] The plant lactobacillus used in this example is commercially available plant lactobacillus Lactobacillus plantarum CICC21804.

[0075] [Example 8]

[0076] The difference between this embodiment and embodiment 6 is that:

[0077] The fermentation method of the plant extract of this embodiment is as follows: 1wt% glucose, 1wt% maltose, and 2wt% tryptone are added to the plant extract, and 5% of the composite fermentation strain is inoculated after sterilization, and cultured at 30°C and 150r / min for 48h, sterilized, cooled to room temperature, and centrifuged to obtain a supernatant;

[0078] The composite fermentation bacteria are yeast, Monascus, and Lactobacillus plantarum, with a mass ratio of 1:0.3:0.2.

[0079] The yeast used in this example is the commercially available Trichosporon sp.CICC 31755;

[0080] The Monascus used in this example is commercially available Monascus sp. CICC 40712.

[0081] The plant lactobacillus used in this example is commercially available plant lactobacillus Lactobacillus plantarum CICC21804.

[0082] [Comparative Example 1]

[0083] Compared with Example 3, the difference is that it does not contain Sichuan pepper, and the other ingredients are the same as those in Example 1, and the mass ratio of each ingredient is adjusted to be the same as that in Example 1.

[0084] [Comparative Example 2]

[0085] Compared with Example 3, the difference is that Lithospermum officinale is not contained, the other components are the same as those in Example 1, and the mass ratios of the components are adjusted to be the same as those in Example 1.

[0086] [Comparative Example 3]

[0087] Compared with Example 3, the difference is that the coix seeds are replaced with the same mass fraction of mulberry bark.

[0088] [Comparative Example 4]

[0089] Compared with Example 7, the difference is that the composite fermentation bacteria are yeast and Monascus, and the mass ratio is 1:0.3.

[0090] [Comparative Example 5]

[0091] Compared with Example 7, the difference is that the composite fermentation bacteria are yeast and Lactobacillus plantarum, and the mass ratio is 1:0.4.

[0092] [Comparative Example 6]

[0093] Compared with Example 7, the difference is that the composite fermentation bacteria are only yeast.

[0094] The embodiments and comparative examples are characterized as follows:

[0095]

Representation 1

[0096] Means of detecting skin microcirculation: blood perfusion volume, using laser Doppler blood perfusion imaging.

[0097] Test site temperature: (24±1)℃, relative humidity 45%±5%.

[0098] Subjects: At least 3 females aged 25-35 years old, with non-sensitive skin. The test started at the same time every day and one sample was tested every day.

[0099] Test method: Place a laser Doppler detection probe on the middle nail wrinkle of the subject, apply 0.1g of the experimental sample of the smear cream to the middle nail wrinkle of the subject's marked position, start recording after stabilization for 5 minutes, and use this value as the basic value, and then record once every 5 minutes, each time for 1 minute, and take the average value, and observe for a total of 30 minutes;

[0100] SPSS19 was used as the statistical software, and the data were expressed as mean ± standard deviation. A statistically significant level of sig less than 0.05 indicated a significant difference.

[0101]

[0102] Compared with the baseline value, * represents sig less than 0.05, and ** represents sig less than 0.01.

[0103] From the experimental data in the above table, we can analyze:

[0104] It can be seen from the data of Examples 1-8 that the ointment obtained in the present application shows significant differences in different time periods, proving that the ointment of the present application can effectively promote microcirculation.

[0105] Among Examples 1-5, the results of Examples 3 and 4 are more prominent, and the blood perfusion volume is significantly higher than that of other Examples in each time period; the smear cream sample of Example 3 begins to show a very significant difference (sig less than 0.01) 15 minutes after application, and the difference can be maintained; the smear cream sample of Example 4 begins to show a very significant difference (sig less than 0.01) 20 minutes after application, and the difference can be maintained.

[0106] The blood perfusion of Examples 6-8 is significantly higher than that of Example 3, indicating that fermentation of plant extracts with microorganisms can further promote microcirculation. The data of Example 7 is significantly better than those of the other two examples, indicating that the selection of microorganisms, the ratio of different microorganisms, the coordination of carbon sources, nitrogen sources and other factors during the fermentation process will have an impact on the results. In addition, the ointment sample of Example 7 began to change significantly after 10 minutes of application.

[0107] By comparing the experimental results of Examples 1-3 with Example 3, it can be seen that the selection of plant extract raw materials has a great influence on the results. The various plant extracts of the present application have a very good compatibility relationship and cooperate with each other to obtain the best effect.

[0108] It can be seen from the comparative experimental results of Examples 4-6 and Example 7 that the selection of bacterial species during the fermentation process will also have a significant impact on the results. The best results can be obtained only when the three bacterial species of yeast, Monascus, and Lactobacillus plantarum are properly matched.

[0109]

Representation 2

[0110] The obtained product was subjected to stability test: 10 g of the cream was taken into a centrifuge tube at room temperature, and the stratification time and the spreadability of the sample after stratification were observed on a centrifuge at a speed of 3000 r / min. The spreadability was scored from 1 to 10, with 10 being the best and 1 being the worst.

[0111]

[0112]

[0113] The present application evaluates the product stability of the prepared smear cream samples through centrifugation experiments and spreadability experiments. From the experimental results, the smear creams obtained in Examples 1-8 of the present application have good stability, especially the results of Examples 3, 4, and 6-8 are relatively excellent, which fully demonstrates that the selection of plant extract raw materials and the content ratio have an impact on the results.

[0114] The results of Examples 6-8 show that after the plant extracts are fermented, the products are more stable.

[0115] It can also be seen from the results of Comparative Examples 4-6 that the selection of fermentation microorganisms and the selection of content ratios will affect the results.

[0116] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

[0117] The above is only an embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. A smear cream for promoting microcirculation, characterized in that: The method comprises the following raw materials in parts by weight: plant extract, 15-30 parts of oil phase, and 5-15 parts of emulsifier; The plant extract is composed of the following raw materials in parts by weight: 5-12 parts of safflower, 4-8 parts of prickly ash, 5-9 parts of cyperus rotundus, 3-6 parts of lithospermum officinale, 3-8 parts of salvia miltiorrhiza, and 1-3 parts of coix seed; The plant extract is subjected to fermentation treatment, and the fermentation treatment method is as follows: adding a carbon source and a nitrogen source to the plant extract, adding a composite fermentation strain after sterilization, culturing for 48 hours under the conditions of 25-30°C and 150-200r / min, sterilizing, cooling to room temperature, and centrifuging to obtain a supernatant; The inoculation amount of the composite fermentation strain is 2-8%; The composite fermentation bacteria are yeast, Monascus, and Lactobacillus plantarum, and the mass ratio of the yeast, Monascus, and Lactobacillus plantarum is 1: (0.1-0.3): (0.1-0.4); The carbon source is one or more of glucose, fructose, maltose, and sucrose, and the added amount of the carbon source is 2-4% of the plant extract; The nitrogen source is one or more of casein, trypsin, and ammonium chloride, and the added amount of the nitrogen source is 2-4% of the plant extract.

2. The ointment for promoting microcirculation according to claim 1, characterized in that: The oil phase is one or more of white vaseline, stearyl alcohol, and liquid paraffin; The emulsifier is one or more of sodium lauryl sulfate, Span-80, and sodium stearate.

3. The ointment for promoting microcirculation according to claim 2, characterized in that: The invention is composed of the following raw materials in parts by weight: 9 parts of safflower, 4 parts of Zanthoxylum bungeanum, 6 parts of Vaccinium myrtillus, 4 parts of Lithospermum officinale, 3 parts of Salvia miltiorrhiza, 2 parts of Coix seeds, 10 parts of white vaseline, 8 parts of octadecyl alcohol and 10 parts of sodium stearate.

4. The ointment for promoting microcirculation according to claim 1, characterized in that: The invention also includes one or more of the following raw materials in parts by weight: 5-8 parts of moisturizing agent, 0.01-0.1 parts of preservative, 0.2-1 parts of antioxidant, and 0.1-0.3 parts of thickener.

5. The ointment for promoting microcirculation according to claim 4, characterized in that: The moisturizing agent is one or more of propylene glycol, glycerin and urea; The preservative is one or more of ethyl p-hydroxybenzoate and salicylic acid; The antioxidant is one or more of vitamin C and sodium sulfite; The thickener is one or more of behenyl alcohol, xanthan gum and hydroxyethyl cellulose.

6. The ointment for promoting microcirculation according to claim 1, characterized in that: The preparation method of the plant extract is as follows: after crushing the raw material of the plant extract, deionized water is added to the raw material at a material-liquid ratio of 1: (20-40), and after stirring and soaking for 30-120 minutes, it is placed in a water bath pot at 50-60°C for 2-3 hours and then filtered to obtain the plant extract.

7. A method for preparing the microcirculation promoting ointment according to any one of claims 1 to 6, characterized in that: The steps include: (1) Heat and melt the oil phase and other substances that are not easily soluble in water, and stir evenly; (2) Heat the plant extract and water-soluble substances to dissolve, add 50 ml of water and stir well; (3) Slowly add the solution obtained in step (2) into the liquid obtained in step (1), and stir until condensed to obtain a smear paste.

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