Preparation method and use of anti-aging product

Abstract

The invention discloses a preparation method and use of an anti-aging product. The preparation method of the product is as follows: (1) preparing a ganoderma lucidum extract: taking a dried ganoderma lucidum fruiting body, crushing, leaching, adding water at 100±5°C according to a material-liquid mass ratio of 1:8-10, and repeatedly extracting at least three times, combining the extract, concentrating, and drying to obtain an extract; (2) preparing a versicolor extract: taking a dried versicolor fruiting body, crushing, leaching, adding water at 100±5°C and repeatedly extracting at least three times, combining the extract, concentrating, and drying to obtain an extract; (3) mixing 35-42 parts of β-nicotinamide mononucleotide, 13-16 parts of ganoderma lucidum extract, 18-21 parts of cordyceps sinensis or its extract, 6-10 parts of coenzyme Q10, 15-22 parts of versicolor extract, and 1.5-2.5 parts of yeast by weight. The preparation method provided by the invention is simple and easy to operate, easy to implement industrially, and the obtained product significantly reduces the cell aging rate, maintains telomerase activity, and has a good anti-aging effect.

Description

Technical Field

[0001] The present invention relates to the technical field of anti-aging products, and in particular to a preparation method and application of an anti-aging product. Background Art

[0002] Aging is a process in which multiple factors lead to the gradual decline of cell, tissue and organism functions. It is caused by the combined effects of multiple factors such as cell decline, DNA degradation, diet and mental factors, and active aging groups. The scientific community has been committed to finding ways to slow down aging. As the problem of global population aging becomes more and more serious, how to delay aging has become a hot topic in the field of modern gerontology research. Aging can be divided into physiological aging and pathological aging. The former refers to the state of natural aging of body functions and metabolism over time, and the latter refers to the aging state caused by various diseases. Physiological aging occurs in all individuals, so it is more concerned.

[0003] As a traditional medicine, Chinese medicine has been widely used in the field of anti-aging. Many Chinese medicinal materials are considered to have anti-aging potential, such as ginseng, astragalus, Panax notoginseng, etc. However, the anti-aging effect of a single Chinese medicinal material is limited, and the anti-aging effect cannot achieve satisfactory results. Therefore, researchers have begun to pay attention to the development of compound Chinese medicine products. Compound products are a reasonable combination of active ingredients of multiple Chinese medicinal materials or active ingredients of Chinese medicine with Western modern scientific and technological ingredients in order to achieve more significant anti-aging effects. The development of compound products requires in-depth research on the active ingredients in Chinese medicinal materials and the evaluation of their interactions and synergistic effects. However, due to the complexity of the active ingredients of Chinese medicine and the differences in the active ingredients of different Chinese medicines, how to screen and obtain anti-aging products with good compound effects is a problem that technicians need to solve. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a preparation method and use of an anti-aging product. The product of the present invention is refined by giving equal importance to traditional Chinese and Western medicine and combining traditional Chinese and Western medicines, and forms a composite anti-aging product prepared by combining Yunzhi extract, Ganoderma lucidum extract, Cordyceps sinensis with NMN, coenzyme Q10, and selenium-enriched yeast.

[0005] The technical solution of the present invention is as follows:

[0006] A method for preparing an anti-aging product comprises the following steps:

[0007] (1) Preparation of Ganoderma lucidum extract: taking dried Ganoderma lucidum fruiting bodies, crushing, eluting, adding water at a mass ratio of 1:8-10 at 100±5°C, and extracting repeatedly for at least three times, combining the extracts, concentrating, and drying to obtain an extract;

[0008] (2) Preparation of Yunzhi extract: Take dried Yunzhi fruiting bodies, crush, rinse, add water at 100±5℃ and extract repeatedly for at least three times, combine the extracts, concentrate, and dry to obtain an extract;

[0009] (3) Pretreatment of Cordyceps sinensis: remove impurities, spread the material on each plate with a thickness of 0.5-1 cm, set the temperature to 55±5℃, set the drying time to 20-40 minutes, and the moisture content of the material after drying shall not exceed 7.0%. After drying, ozone sterilization shall be carried out, and then crushing and sieving shall be carried out;

[0010] (4) By weight, 35-42 parts of β-nicotinamide mononucleotide, 13-16 parts of Ganoderma lucidum extract, 18-21 parts of Cordyceps sinensis or its extract, 6-10 parts of coenzyme Q10, 15-22 parts of Versicolor versicolor extract, and 1.5-2.5 parts of yeast are mixed. This formula is the preferred formula. Studies have shown that the anti-aging effect of this Chinese and Western medicine composite product is better than that of a single ingredient.

[0011] Preferably, by weight, the ratio of the components in the anti-aging product is: 38.10 parts of β-nicotinamide mononucleotide, 14.01 parts of wild Ganoderma lucidum extract, 19.05 parts of wild Cordyceps sinensis or its extract, 7.41 parts of coenzyme Q10, 19.64 parts of wild Versicolor extract, and 1.79 parts of selenium-enriched yeast.

[0012] Preferably, in step (1), water is added for extraction three times, each time for extraction for 2 hours, the amount of water added for the first and second times is 10 times, and the amount of water added for the third time is 8 times; in step (2), water is added for extraction three times, each time for extraction for 2 hours, and the amount of water added for each time is 10 times.

[0013] Preferably, the preparation method of Cordyceps sinensis extract includes: taking the pretreated Cordyceps sinensis powder, adding water, adjusting the pH to 8.5±0.2, extracting at 40±2°C for at least 2h, centrifuging, adjusting the pH of the supernatant obtained by centrifugation to 3.8±0.2, and collecting the precipitate by centrifugation; making the precipitate into a solution, adding pepsin and papain, adjusting the pH to 5.5±0.2, enzymolysis, inactivating the enzyme, separating the solid from the liquid, taking the supernatant, ultrafiltration, obtaining the product, and freeze-drying.

[0014] Preferably, the preparation method of Cordyceps sinensis extract comprises:

[0015] Take the pretreated Cordyceps sinensis powder, add water, adjust the pH to 8.5±0.2, extract at 40±2°C for at least 2h, centrifuge, adjust the pH of the supernatant obtained by centrifugation to 3.8±0.2 with hydrochloric acid solution, and collect the precipitate by centrifugation; use water to make the precipitate into a solution, add pepsin and papain, adjust the pH to 5.5±0.2, enzymolysis, inactivate the enzyme, separate the solid and liquid, take the supernatant, ultrafilter, obtain a product with a molecular weight of ≤6000Da, freeze-dry it, and set it aside.

[0016] Preferably, the mass ratio of pepsin to papain is 1:1.

[0017] On the other hand, the present invention relates to an anti-aging product obtained by the preparation method.

[0018] The present invention relates to the use of the preparation method or the product in preparing a product for improving the activity of an anti-aging enzyme. The anti-aging enzyme includes telomerase.

[0019] At present, the main theories about skin aging include: DNA damage and repair theory, cell apoptosis theory, free radical theory, telomerase theory, gene regulation theory, immune theory, neuroendocrine theory, etc. Based on these theories, the anti-aging approaches mainly start from improving the body's immune function, anti-oxidation, DNA oxidative damage protection, inhibiting cell apoptosis, and increasing the activity of anti-aging enzymes.

[0020] The telomere theory believes that the length of telomeres at the ends of eukaryotic chromosomes is closely related to the aging of the body. Telomere DNA continues to shorten with the increase in the number of cell divisions. When it shortens to a certain extent, the telomere function is disordered, the chromosome loses protection, and eventually leads to the aging of the body. Telomere length is controlled by telomerase activity. When telomerase activity is enhanced, the longer the telomere, the better the stability and integrity of the chromosome, thereby extending the life of the body. The research results show that the product prepared by the present invention has a good promoting effect on telomerase activity, effectively maintains the telomerase activity of cells that have been passaged for multiple times, and alleviates the aging process.

[0021] The present invention further relates to the use of the preparation method or the product in the preparation of anti-aging products. The products include health foods and medicines. The dosage forms of the products include capsules, tablets, freeze-dried powders, and liquid preparations.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The preparation method provided by the present invention is simple and easy to operate and to be industrially implemented. The product formula has fewer ingredients but has obvious anti-aging effects, can meet market demand and is conducive to controlling production costs.

[0024] Chinese herbal medicines such as Ganoderma lucidum, Versicolor, and Cordyceps sinensis contain a variety of functional ingredients, each with different effects. The present invention rationally combines the extracted and purified Versicolor extract, Ganoderma lucidum extract, and Cordyceps sinensis extract with NMN, coenzyme Q10, and selenium-enriched yeast. The resulting product significantly reduces the cell aging rate, maintains telomerase activity, and has a good anti-aging effect. DETAILED DESCRIPTION

[0025] In order to enable those skilled in the art to more fully understand the technical content of the present invention, the present invention is described in detail below through specific implementation cases and experimental research results.

[0026] In the following examples, the versicolor described in the present invention is a fungus of the family Polyporaceae, Coriolus Versicolor (L.exFr.) Quel dry fruiting body; the Ganoderma lucidum is a fungus of the genus Ganoderma in the family Polyporaceae Ganoderma lucidum (Leyss. ex Fr.) Karst. or Ganoderma lucidum Ganoderma sinense Zhao, Xu et Zhang's dry fruiting body. The Cordyceps sinensis is a fungus of the genus Cordyceps of the family Ergotaceae. Cordyceps sinensis The complex of the (BerK.) Sacc stroma and its host bat moth larvae (sclerotia). The selenium-enriched yeast is a light yellow powder containing 300 mg / kg of selenium. The yeast is cultured in a culture medium containing inorganic selenium (such as sodium selenite). It is a commercially available product. β-Nicotinamide mononucleotide, coenzyme Q10, etc. are all commercially available products.

[0027] Pepsin, enzyme activity 250 U / mg, powder; Papain, 100,000 U / g, powder.

[0028] Example 1

[0029] A method for preparing an anti-aging product comprises the following steps:

[0030] (1) Preparation of wild Ganoderma lucidum extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, the amount of water added in the first and second extractions is 10 times, and the third extraction is 8 times), combine the extracts, concentrate, and dry them to obtain an extract;

[0031] (2) Preparation of wild Yunzhi extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, and the amount of water added each time is 10 times), combine the extracts, concentrate, and dry them to obtain the extract.

[0032] (3) Pretreatment of Cordyceps sinensis: remove impurities, spread the material on each plate at a thickness of 0.5-1 cm, set the temperature to 55±5℃, set the drying time to 20-40 minutes, and the moisture content of the material after drying should not exceed 7.0%. After drying, perform ozone sterilization, then crush and pass through a 100-mesh sieve;

[0033] (4) 14.01 parts of wild Ganoderma lucidum extract, 19.64 parts of wild Versicolor extract, 38.10 parts of β-nicotinamide mononucleotide (NMN), 19.05 parts of wild Cordyceps sinensis, 7.41 parts of coenzyme Q10, and 1.79 parts of selenium-enriched yeast were crushed and mixed, passed through a 100-mesh sieve, and filled into capsule shells to prepare capsules.

[0034] Experiment 1

[0035] This experiment compares the anti-aging effects of the combination and the single ingredient. The preparation method of the extract is as in Example 1. The experimental method is as in Experiment 3. The results are shown in Table 1.

[0036] Table 1

[0037]

[0038] The results in Table 1 show that each component has a certain anti-aging ability. When the components are used in combination, they play a certain role in promoting each other, and the anti-aging effect is better.

[0039] Example 2

[0040] Based on the previous research results, we further improved the product process, isolated the active substances of Cordyceps sinensis, and used them in the composition.

[0041] Method for separating active ingredients of Cordyceps sinensis ①: Take the Cordyceps sinensis powder pretreated in Example 1, add water at a material-liquid ratio of 1g:50mL, adjust the pH to 8.5±0.2 with sodium hydroxide, extract at 40±2°C for at least 2h, centrifuge, adjust the pH of the supernatant obtained by centrifugation to 3.8±2 with hydrochloric acid solution, and collect the precipitate by centrifugation. Use water to make a solution with a mass concentration of 5%, add pepsin and papain (equal mass ratio) according to the enzyme substrate mass ratio of 3%, adjust the pH to 5.5±0.2 with hydrochloric acid solution, enzymolysis for 3h, inactivate the enzyme, centrifuge and take the supernatant, ultrafilter, obtain products with different molecular weights, and freeze-dry the product with a molecular weight of ≤6000Da to obtain an extract for standby use.

[0042] Method ② for separating the active ingredients of Cordyceps sinensis: Take the Cordyceps sinensis powder pretreated in Example 1, add water at a material-liquid ratio of 1g:50mL, adjust the pH to 8.5±0.2 with sodium hydroxide, extract at 40±2°C for at least 2h, centrifuge, adjust the pH of the supernatant obtained by centrifugation to 3.8±2 with hydrochloric acid solution, and collect the precipitate by centrifugation. Use water to make a solution with a mass concentration of 5%, add pepsin and papain (equal mass ratio) according to the enzyme substrate mass ratio of 3%, adjust the pH to 5.5±0.2 with hydrochloric acid solution, enzymolysis for 3h, inactivate the enzyme, centrifuge and take the supernatant, ultrafilter, obtain products of different molecular weights, and freeze-dry the product with a molecular weight of >6000Da to obtain an extract for later use.

[0043] Method for separating the active ingredients of Cordyceps sinensis ③: Take the Cordyceps sinensis powder pretreated in Example 1, add water at a material-liquid ratio of 1g:50mL, adjust the pH to 8.5±0.2 with sodium hydroxide, extract at 40±2℃ for at least 2h, centrifuge, adjust the pH of the supernatant obtained by centrifugation to 3.8±2 with hydrochloric acid solution, and collect the precipitate by centrifugation. Use water to make a solution with a mass concentration of 5%, add alkaline protease (equal mass ratio) according to the enzyme substrate mass ratio of 3%, adjust the pH to 8.5±0.2 with sodium hydroxide solution, enzymolysis for 3h, inactivate the enzyme, centrifuge and take the supernatant, ultrafilter, obtain products with different molecular weights, and freeze-dry the product with a molecular weight of ≤6000Da to obtain an extract for standby use.

[0044] Experiment 2

[0045] The Cordyceps sinensis extract obtained in Example 2 and the Cordyceps sinensis powder pretreated in Example 1 were taken and the percentage of senescent cells and telomerase activity were detected according to the method of Experiment 3. The results are shown in Table 2.

[0046] Table 2

[0047]

[0048] The results in Table 2 show that the product obtained by method ① has the strongest anti-aging ability.

[0049] Example 3

[0050] The product obtained by method ① of Example 2 was used in the product of Example 1. The steps for preparing the product are as follows:

[0051] A method for preparing an anti-aging product comprises the following steps:

[0052] (1) Preparation of wild Ganoderma lucidum extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, the amount of water added in the first and second extractions is 10 times, and the third extraction is 8 times), combine the extracts, concentrate, and dry them to obtain an extract;

[0053] (2) Preparation of wild Yunzhi extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, and the amount of water added each time is 10 times), combine the extracts, concentrate, and dry them to obtain the extract.

[0054] (3) Pretreatment of Cordyceps sinensis: remove impurities, spread the material on each plate to a thickness of 0.5-1 cm, set the temperature to 55±5°C, set the drying time to 20-40 minutes, and the moisture content of the material after drying shall not exceed 7.0%. After drying, ozone sterilization shall be performed, and then the material shall be crushed and sieved. The extract shall be prepared by the method of method ① of Example 2;

[0055] (4) 14.01 parts of wild Ganoderma lucidum extract, 19.64 parts of wild Versicolor extract, 38.10 parts of β-nicotinamide mononucleotide (NMN), 19.05 parts of wild Cordyceps sinensis extract, 7.41 parts of coenzyme Q10, and 1.79 parts of selenium-enriched yeast were crushed and mixed, passed through a 100-mesh sieve, and filled into capsule shells to prepare capsules.

[0056] Example 4

[0057] A method for preparing an anti-aging product comprises the following steps:

[0058] (1) Preparation of wild Ganoderma lucidum extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, the amount of water added in the first and second extractions is 10 times, and the third extraction is 8 times), combine the extracts, concentrate, and dry them to obtain an extract;

[0059] (2) Preparation of wild Yunzhi extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, and the amount of water added each time is 10 times), combine the extracts, concentrate, and dry them to obtain the extract.

[0060] (3) Pretreatment of Cordyceps sinensis: remove impurities, spread the material on each plate with a thickness of 0.5-1 cm, set the temperature to 55±5°C, set the drying time to 20-40 minutes, and the moisture content of the material after drying shall not exceed 7.0%. After drying, ozone sterilization shall be performed, and then the material shall be crushed and passed through a 100-mesh sieve; the extract shall be prepared by the method of method ① of Example 2;

[0061] (4) 13 parts of wild Ganoderma lucidum extract, 22 parts of wild Versicolor extract, 35 parts of β-nicotinamide mononucleotide (NMN), 18 parts of wild Cordyceps sinensis extract, 10 parts of coenzyme Q10, and 2.5 parts of selenium-enriched yeast were crushed and mixed, passed through a 100-mesh sieve, and filled into capsule shells to prepare capsules.

[0062] Example 5

[0063] A method for preparing an anti-aging product comprises the following steps:

[0064] (1) Preparation of wild Ganoderma lucidum extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, the amount of water added in the first and second extractions is 10 times, and the third extraction is 8 times), combine the extracts, concentrate, and dry them to obtain an extract;

[0065] (2) Preparation of wild Yunzhi extract: Take dried Ganoderma lucidum fruiting bodies, crush them, rinse them, add water at 100±5°C at a material-liquid mass ratio of 1:8-10, and extract them three times (each extraction takes 2 hours, and the amount of water added each time is 10 times), combine the extracts, concentrate, and dry them to obtain the extract.

[0066] (3) Pretreatment of Cordyceps sinensis: remove impurities, spread the material on each plate with a thickness of 0.5-1 cm, set the temperature to 55±5°C, set the drying time to 20-40 minutes, and the moisture content of the material after drying shall not exceed 7.0%. After drying, ozone sterilization shall be performed, and then the material shall be crushed and passed through a 100-mesh sieve; the extract shall be prepared by the method of method ① of Example 2;

[0067] (4) 16 parts of wild Ganoderma lucidum extract, 15 parts of wild Yunzhi extract, 42 parts of β-nicotinamide mononucleotide (NMN), 21 parts of wild Cordyceps sinensis extract, 6 parts of coenzyme Q10, and 1.5 parts of selenium-enriched yeast were crushed and mixed, passed through a 100-mesh sieve, and filled into capsule shells to prepare capsules.

[0068] Experiment 3

[0069] The present invention screens out the most suitable dosage range of the prescription, which is: 35-42 parts of beta-nicotinamide mononucleotide, 13-16 parts of wild ganoderma lucidum extract, 18-21 parts of wild cordyceps sinensis extract, 6-10 parts of coenzyme Q10, 15-22 parts of wild versicolor extract, and 1.5-2.5 parts of selenium-enriched yeast.

[0070] The present invention has respectively measured the anti-aging effects of products under different ratios. The following are the experimental methods and some experimental results.

[0071] Experimental method: The sample to be tested was added to the human skin fibroblast culture medium at a concentration of 100 mg / L, and subcultured. The blank control group was not added with the sample to be tested. After 10 generations, the β-galactosidase staining kit was used for detection. The detection method was in accordance with the kit instructions. The number of staining positive cells was recorded and the percentage of senescent cells was calculated. The TRAP-ELISA kit was used to detect telomerase activity. The detection method was in accordance with the kit instructions. P <0.01 indicates significant difference compared with the blank group.

[0072] Table 3

[0073] Group Percentage of senescent cells value Administration group (Example 3) 29.9±1.5 ＜0.01 Administration group (Example 4) 32.1±3.1 ＜0.01 Administration group (Example 5) 33.6±2.7 ＜0.01 Blank control group 66.2±2.8 -

[0074] Table 4

[0075] Group Telomerase activity value Administration group (Example 3) 0.463±0.013 ＜0.01 Administration group (Example 4) 0.432±0.035 ＜0.01 Administration group (Example 5) 0.4295±0.026 ＜0.01 Blank control group 0.184±0.013 -

[0076] The results showed that after the intervention of the product of the present invention, the percentage of senescent cells decreased significantly compared with the control group, and the telomerase activity increased significantly. The product of the present invention has an obvious anti-aging effect.

[0077] The above description is only a specific implementation mode of the present invention, not all of the present invention. For those skilled in the art, improvements in different forms and degrees can be made on the basis of the present invention. All modifications, equivalent substitutions, etc. made within the spirit and principles of the present invention fall within the scope of the present invention.

Claims

1. A method for preparing an anti-aging product, characterized in that: The following steps are involved: (1) Preparation of Ganoderma lucidum extract: taking dried Ganoderma lucidum fruiting bodies, crushing, eluting, adding water at a mass ratio of 1:8-10 at 100±5°C, and extracting repeatedly for at least three times, combining the extracts, concentrating, and drying to obtain an extract; (2) Preparation of Yunzhi extract: Take dried Yunzhi fruiting bodies, crush, rinse, add water at 100±5℃ and extract repeatedly for at least three times, combine the extracts, concentrate, and dry to obtain an extract; (3) Pretreatment of Cordyceps sinensis: remove impurities, spread the material on each plate with a thickness of 0.5-1 cm, set the temperature to 55±5℃, set the drying time to 20-40 minutes, and the moisture content of the material after drying shall not exceed 7.0%. After drying, ozone sterilization shall be carried out, and then crushing and sieving shall be carried out; (4) Mix, by weight, 35-42 parts of β-nicotinamide mononucleotide, 13-16 parts of Ganoderma lucidum extract, 18-21 parts of Cordyceps sinensis or its extract, 6-10 parts of coenzyme Q10, 15-22 parts of Versicolor versicolor extract, and 1.5-2.5 parts of yeast; The preparation method of Cordyceps sinensis extract comprises: taking pre-treated Cordyceps sinensis powder, adding water, adjusting the pH to 8.5±0.2, extracting at 40±2°C for at least 2h, centrifuging, adjusting the pH of the supernatant obtained by centrifugation to 3.8±0.2 with a hydrochloric acid solution, and collecting the precipitate by centrifugation; making the precipitate into a solution with water, adding pepsin and papain, adjusting the pH to 5.5±0.2, enzymolysis, inactivating the enzyme, separating the solid from the liquid, taking the supernatant, ultrafiltration, obtaining a product with a molecular weight of ≤6000Da, freeze-drying, and setting aside.

2. The method for preparing an anti-aging product according to claim 1, characterized in that: The proportions of the components are as follows by weight: 38.10 parts of β-nicotinamide mononucleotide, 14.01 parts of wild Ganoderma lucidum extract, 19.05 parts of wild Cordyceps sinensis or its extract, 7.41 parts of coenzyme Q10, 19.64 parts of wild Versicolor extract, and 1.79 parts of selenium-enriched yeast.

3. The method for preparing an anti-aging product according to claim 1, characterized in that: In step (1), water is added for extraction three times, each time for 2 hours, the amount of water added for the first and second times is 10 times, and the amount of water added for the third time is 8 times; in step (2), water is added for extraction three times, each time for 2 hours, and the amount of water added for each time is 10 times.

4. The method for preparing an anti-aging product according to claim 3, characterized in that: The mass ratio of pepsin to papain is 1:

1.

5. An anti-aging product prepared by the preparation method according to any one of claims 1 to 4.

6. Use of the product according to claim 5 in the preparation of anti-aging products.

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