A concentrated solution preparation process after extracting sweet glycosides and the concentrated solution and application

Abstract

The present invention relates to the technical field of concentrated solution preparation, and in particular to a concentrated solution preparation process after the extraction of mogrosides, a concentrated solution, and an application. The concentrated solution preparation process after the extraction of mogrosides comprises the following steps: S1. The mogroside aqueous solution passes through an adsorption resin column, and the chromatographic solution discharged from the adsorption resin column is collected; S2. The chromatographic solution is concentrated by vacuum decompression to obtain a semi-finished concentrated solution with a specific gravity of 1.12-1.39; S3. The semi-finished concentrated solution is sterilized and cooled to obtain the concentrated solution. The eluate discharged from the mogroside adsorption resin column is used as a raw material, and one fruit is used for multiple purposes, which increases the added value of monk fruit, reduces the raw material purchase cost at the breeding end, and is beneficial to the investment in the breeding industry; the preparation process is simple, and the discharged concentrated steam has no impact on the environment, saving process equipment investment and production costs; the above-mentioned concentrated solution has an outstanding use effect in breeding, and when the addition amount is only 0.1%-0.5%, it has a good improvement on the breeding effect.

Description

Technical Field

[0001] The invention relates to the technical field of concentrated solution preparation, and in particular to a concentrated solution preparation process after sweet glycosides are extracted, the concentrated solution and application. Background Art

[0002] Monk fruit is a very good natural plant or Chinese medicinal material, mostly produced in Guilin, Guangxi Zhuang Autonomous Region. It is widely used in the field of traditional Chinese medicine because of its effects of relieving asthma and ventilation, improving diet, diuresis and laxative, and clearing the lungs and relieving heat. It can also be soaked in water and drunk in daily life. Because Monk fruit contains good mogroside, its sweetness is about 300 times that of sucrose, and its long-term use will not cause obesity like sucrose. Therefore, it is gradually popular in Europe and the United States to use mogroside to replace sucrose. Chinese plant extract companies extract mogroside and export it to Europe and the United States. However, the treatment of the extraction chromatography liquid after extracting mogroside is laborious. At present, there is no better treatment method, and it is now mostly treated as wastewater.

[0003] In recent years, the Chinese herbal medicine after the effective ingredient is extracted is crushed or fermented and added to the feed for application, which has also been promoted. However, the cost of directly using the Chinese herbal medicine extract for treatment or breeding is high, and it is also impossible to guarantee that the Chinese herbal medicine extract has a good effect of improving breeding income. Existing research, such as invention patents CN106923113A, CN107586820A, CN110105458A, CN112244185A, CN115417847A, and CN115873059A, provides some methods for extracting health products or concentrated juice from mogroside extraction chromatography fluid, but they are mainly used for human food or medical health care, and the above extraction process is relatively complicated, requiring the investment of more new equipment, and the use of the above process will generate more environmental protection and waste liquid treatment pressure in the specific preparation process. Summary of the invention

[0004] In order to meet the needs of reducing the cost of existing breeding and fully realize the multiple uses of one fruit / one plant to enhance the value and use of the plant itself, the first object of the present invention is to provide a preparation process for producing a concentrated solution from a chromatography liquid extracted with mogroside and a concentrated solution, the concentrated solution uses the eluate discharged from a mogroside adsorption resin column as a raw material, which is equivalent to multiple uses of one fruit, thereby increasing the added value of monk fruit, reducing the purchase cost of raw materials at the breeding end, and reducing the investment in breeding; in addition, the preparation process of the concentrated solution is relatively simple, the chromatography liquid can be directly concentrated, and the discharged concentrated steam has no effect on the environment, which greatly reduces the pressure of sewage treatment, protects the environment and saves process equipment investment and production costs.

[0005] To achieve the above object, the present invention provides a process for preparing a concentrated solution after extracting sweet glycosides, comprising the following steps:

[0006] S1. The mogroside aqueous solution passes through an adsorption resin column, and the chromatographic liquid discharged from the adsorption resin column is collected;

[0007] S2. The chromatography fluid is concentrated under vacuum to obtain a semi-finished concentrate having a specific gravity of 1.12-1.39;

[0008] S3. The semi-finished concentrated liquid is sterilized and cooled to obtain the concentrated liquid.

[0009] The raw material for the production of the concentrated solution of this scheme is directly the mogroside extraction chromatography liquid. The obtained concentrated solution can be used as an additive for breeding, which can better improve the breeding effect, realize the reuse of waste liquid, and is more environmentally friendly. The preparation process of the above concentrated solution is directly concentrated and evaporated by the mogroside extraction chromatography liquid, and the Brix sugar content is used as the end index of the concentration process. The index is simple and easy to operate.

[0010] Preferably, in step S2, the vacuum degree during vacuum decompression concentration is 0.06-0.08 MPa, the concentration temperature is ≤70°C, the concentration time is 90-120 min, and the vacuum decompression concentration is performed at a ratio of 30-40:1 between the chromatographic liquid and the concentrated liquid.

[0011] Preferably, in step S3, cooling is performed at room temperature, and generally room temperature refers to 20°C±5°C.

[0012] Preferably, the sterilization process in step S4 is high temperature sterilization at 108-112°C and a flow rate of 0.8-1.0 m 3 / h, 3-8 seconds instant sterilization.

[0013] Preferably, in step S1, the pH value of the obtained chromatographic solution is 4.5-5.5.

[0014] Preferably, the step S2 comprises the following steps: the chromatography liquid and the concentrated liquid are concentrated under vacuum at a ratio of 33-38:1 to obtain a semi-finished concentrated liquid with a specific gravity of 1.18-1.35.

[0015] Under the above parameter limitations, the concentrated liquid obtained, when used as an aquaculture additive, still has a good aquaculture improvement effect under the premise of a small dosage.

[0016] Preferably, step S2 comprises the following steps: the chromatographic liquid and the concentrated liquid are concentrated under vacuum at a ratio of 35:1 to obtain a semi-finished concentrated liquid with a specific gravity of 1.22-1.30. Under the above parameter limits, the concentrated liquid obtained has the best aquaculture improvement effect.

[0017] The present invention also provides a concentrated solution, which is prepared by any of the above-mentioned concentrated solution preparation processes after the glycosides are extracted.

[0018] In addition, the second purpose of the present invention is to provide an application of the above-mentioned concentrated solution produced from the mogroside extraction chromatography liquid in livestock and poultry and aquaculture. The concentrated solution can be directly added to drinking water as an additive for animals to eat, and can be well applied to various modern aquaculture intelligent drinking water systems, without blocking the water line, convenient medication, and low labor intensity.

[0019] Preferably, the amount of the concentrate used is 0.1%-0.5% of the water mass. The concentrate has an outstanding use effect, and when the addition amount is 0.1%-0.5%, it can show a good breeding improvement effect and increase the breeding efficiency.

[0020] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0021] (1) The concentrated liquid produced by this process is used in the production of livestock and poultry. The eluate discharged from the mogroside adsorption resin column is used as the raw material, which is equivalent to using one fruit for multiple purposes, thereby increasing the added value of monk fruit and reducing the raw material purchase cost at the breeding end, which is beneficial to the investment in the breeding industry.

[0022] (2) The preparation process is simple, the chromatography liquid is directly concentrated, and the discharged concentrated steam has no impact on the environment, which greatly reduces the pressure of sewage treatment, protects the environment and saves process equipment investment and production costs;

[0023] (3) The concentrated solution prepared by the present invention is easy to use and can be applied to various modern aquaculture intelligent drinking water systems. It does not block the water line, is easy to administer, and has low labor intensity.

[0024] (4) The concentrated liquid prepared by the present invention has outstanding use effect in aquaculture, and the aquaculture effect can be improved significantly when the addition amount is only 0.1%-0.5%. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0027] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0028] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → When the temperature is below 70°C, vacuum concentrate the chromatographic liquid and the concentrated liquid at a ratio of 30-40:1 (vacuum degree is 0.06-0.08MPa, and the concentration time is 90-120min) to obtain a semi-finished concentrated liquid with a specific gravity of 1.12-1.39 → high temperature of 108-112°C, flow rate of 0.8-1.0m 3 / h, 3-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0029] The concentrated liquid product prepared above is added with 1-5L of the product per ton of water (that is, the added amount can be adjusted within the range of 0.1%-0.5%, and the added amount is determined according to the specific situation) for livestock and poultry aquaculture.

[0030] Or, collect the chromatographic liquid discharged from the mogroside adsorption resin column → vacuum decompression and concentration at a ratio of 33-38:1 below 70°C (vacuum degree is 0.06-0.08MPa, concentration time is 90-120min) to obtain a semi-finished concentrated liquid with a specific gravity of 1.18-1.35 → high temperature of 108-112°C, flow rate of 0.8-1.0m 3 / h, 3-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0031] The above-prepared concentrated liquid product can be added with 2-4L of this product per ton of water (that is, the addition amount can be adjusted within the range of 0.2%-0.4%, and the addition amount is determined according to the specific situation) for livestock and poultry aquaculture, which can effectively reduce the feed-to-meat ratio and improve production performance.

[0032] Or, collect the chromatographic liquid discharged from the mogroside adsorption resin column → vacuum decompression and concentration at a ratio of 35:1 below 70°C (vacuum degree is 0.06-0.08MPa, concentration time is 90-120min) to obtain a semi-finished concentrated liquid with a specific gravity of 1.22-1.30 → high temperature of 108-112°C, flow rate of 0.8-1.0m 3 / h, 3-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0033] The above-prepared concentrated liquid product can be added with 2-3L of this product per ton of water (that is, the addition amount can be adjusted within the range of 0.2%-0.3%, and the addition amount is determined according to the specific situation) for livestock and poultry aquaculture, which can effectively reduce the feed-to-meat ratio and improve production performance.

[0034] Among them, using the chromatography liquid extracted with mogroside as the raw material for production can reduce the procurement cost of the raw materials, and after the mogroside is extracted, the high sweetness of the concentrated liquid is greatly reduced, and the appropriate sweetness is more conducive to animal feeding; although the mogroside has been extracted, its protein and rich amino acids are still retained in the chromatography liquid, and when the chromatography liquid reaches the special specific gravity specified in this scheme after concentration, the protein and rich amino acids are enriched to a certain concentration, which is more suitable for the nutritional needs and digestion and absorption of animals, thereby promoting animal growth, reducing feed-to-meat ratio, and improving breeding efficiency.

[0035] The technical solution of the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention, and are not used to limit the present invention.

[0036] Example 1

[0037] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0038] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → Concentration temperature ≤ 70°C, vacuum decompression concentration at a ratio of 30:1 (vacuum degree 0.06-0.08MPa, concentration time 100min) to obtain a concentrate with a specific gravity of 1.13 → High temperature 108-112°C, flow rate 0.8-0.9m 3 / h, 5-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0039] Example 2

[0040] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0041] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → Concentration temperature ≤ 70°C, vacuum decompression concentration at a ratio of 33:1 (vacuum degree 0.06-0.08MPa, concentration time 102min) to obtain a concentrate with a specific gravity of 1.18 → High temperature 108-112°C, flow rate 0.8-0.9m 3 / h, 5-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0042] Example 3

[0043] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0044] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → Concentration temperature ≤ 70°C, vacuum decompression concentration at a ratio of 35:1 (vacuum degree 0.06-0.08MPa, concentration time 105min) to obtain a concentrate with a specific gravity of 1.26 → High temperature 108-112°C, flow rate 0.8-0.9m 3 / h, 5-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0045] Example 4

[0046] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0047] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → Concentration temperature ≤ 70°C, vacuum decompression concentration at a ratio of 38:1 (vacuum degree 0.06-0.08MPa, concentration time 105min) to obtain a concentrate with a specific gravity of 1.34 → High temperature 108-112°C, flow rate 0.8-0.9m 3 / h, 5-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0048] Example 5

[0049] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0050] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → Concentration temperature ≤ 70°C, vacuum decompression concentration at a ratio of 40:1 (vacuum degree 0.06-0.08MPa, concentration time 108min) to obtain a concentrate with a specific gravity of 1.39 → High temperature 108-112°C, flow rate 0.8-0.9m 3 / h, 5-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0051] Example 6

[0052] A process for preparing a concentrated solution after extracting sweet glycosides comprises the following steps:

[0053] Collect the chromatographic liquid discharged from the mogroside adsorption resin column → Concentration temperature ≤ 70°C, vacuum decompression concentration at a ratio of 35:1 (vacuum degree 0.06-0.08MPa, concentration time 118min) to obtain a concentrate with a specific gravity of 1.30 → High temperature 108-112°C, flow rate 0.8-0.9m 3 / h, 5-8 seconds instant sterilization → after cooling, filling into concentrated liquid product.

[0054] The concentrated solutions of Examples 1, 3, 4, 5 and 6 were tested for their effects.

[0055] Experimental purpose: To investigate the effect of concentrate on the growth performance of broilers of different ages, including daily weight gain, daily feed intake, and feed-to-meat ratio.

[0056] Experimental subjects: yellow hens or Tianlu hens of different ages, 4 replicates (cages) in each group, 10 chickens in each replicate, each group was fed the basic diet of the corresponding stage, free access to food and water, and routine feeding management and immunization.

[0057] A blank control group without any treatment was set up; each example was used as a test group, and the concentrate prepared by this scheme was used in drinking water.

[0058] Determination index: Determine the relevant indexes of production performance, weigh and record the initial body weight at the beginning of the age, weigh and record the final weight of each repeated stage at the end of the age (stop feeding the night before), and weigh the remaining amount of feed. Carefully observe the health status of the chickens during the entire trial. According to the obtained data, calculate the average daily weight gain and average daily feed intake of each group and each stage to obtain the feed-to-meat ratio.

[0059] Table 1 Effect of the concentrate of Example 1 on 15-35 day old yellow hens

[0060]

[0061] As can be seen from Table 1, the specific gravity of the concentrate of Example 1 is 1.13. Compared with the blank control group, when the concentrate prepared by the present invention is added in a larger amount, that is, 10 kg per ton of water, that is, 1% is added, the feed-to-meat ratio is higher than the blank control by about 0.04, which not only fails to reduce the feed-to-meat ratio, but also has a high cost due to the large amount of the concentrate used as an additive. After the amount of the concentrate is reduced, when 2 kg per ton of water, that is, 0.2% is added, the feed-to-meat ratio is reduced by about 0.2 compared with the blank control group, indicating that the feed-to-meat ratio is reduced under the premise of a small amount of the concentrate, the cost of additives is saved, the feed cost is reduced, and the breeding efficiency is improved.

[0062] Table 2 Effect of the concentrate of Example 3 on 1-7 day old Tianlu Ma hens

[0063]

[0064] It can be seen from Table 2 that the specific gravity of the concentrate of Example 3 is 1.26. Compared with the blank control group, when 5 kg of water is added per ton of water, i.e. 0.5% is added, the feed-to-meat ratio is 0.14 lower, indicating that adding a large dose appropriately can also reduce the feed-to-meat ratio, and the cost of use is not too high; and when 2 kg of water is added per ton of water, i.e. 0.2% is added, the feed-to-meat ratio is reduced by 0.21. The small amount of addition saves the addition cost and can reduce the feed-to-meat ratio to a more ideal range, thereby reducing the feed cost and improving the breeding efficiency.

[0065] Table 3 Effect of the concentrate of Example 6 on 12-23 day old Tianlu Ma hens

[0066]

[0067] It can be seen from Table 3 that the specific gravity of the concentrate of Example 6 is 1.30. Compared with the blank control group, when 5 kg per ton of water, i.e. 0.5%, is added, the feed-to-meat ratio is reduced by 0.08, indicating that the appropriate large dose of addition in this embodiment can reduce the feed-to-meat ratio; and when 2 kg per ton of water, i.e. 0.2% is added, compared with the control group, the feed-to-meat ratio is reduced by 0.15. The small amount of addition effectively saves the addition cost, and can effectively reduce the feed-to-meat ratio and reduce the feed cost; It indicates that the concentrate of Example 6 can improve the breeding efficiency by adding 0.2%-0.5%, i.e. 2-5 kg ​​per ton of water.

[0068] Table 4 Effect of the concentrate of Example 4 on 36-43 day old Tianlu Ma hens

[0069]

[0070] It can be seen from Table 4 that the specific gravity of the concentrate of Example 4 is 1.34. Compared with the blank control group, the feed-to-meat ratio is 0.11 lower when 4 kg is added per ton of water, i.e. 0.4%. This shows that the appropriately large dose of Example 4 has a certain effect on reducing the feed-to-meat ratio; while the feed-to-meat ratio is reduced by 0.14 when 1 kg is added per ton of water, i.e. 0.1%. The small amount of addition effectively saves the addition cost. The concentrate of Example 4 at 0.4%, i.e. 4 kg per ton of water, and at 0.1%, i.e. 1 kg per ton of water, both have the effect of reducing the feed ratio for 36-43 day old Tianlu Ma hens, but the amount of drinking water added at 0.4% is higher than that added at 0.1%, and the effect of reducing the feed ratio is worse than that added at 0.1%. That is, the concentrate within this specific gravity range has a better effect of reducing the feed-to-meat ratio when the amount added is small. In order to improve economic benefits and reduce investment, the concentrate of Example 4 at 0.1% is added.

[0071] ≤Addition amount <0.4%, that is, it is more appropriate to add 1 kg or less than 4 kg per ton of water.

[0072] Table 5 Effect of the concentrate of Example 5 on 50-55 day old Tianlu Ma hens

[0073]

[0074] It can be seen from Table 5 that the specific gravity of the concentrate of Example 5 is 1.39. Compared with the blank control group, the feed-to-meat ratio increases by 0.1 when 5 kg, i.e. 0.5%, is added per ton of water, indicating that the addition of a large dose of a concentrate with a large specific gravity cannot reduce the feed-to-meat ratio and has a high cost of use; and the addition of 2 kg, i.e. 0.2%, per ton of water reduces the feed-to-meat ratio by 0.22 compared with the control group. The small amount of addition effectively saves the addition cost and can effectively reduce the feed-to-meat ratio and reduce the feed cost; indicating that Example 5 is more appropriate when 0.1% ≤ addition amount < 0.5%, i.e. 2 kg and less than 5 kg are added per ton of water.

[0075] Table 6 Effect of Example 6 concentrate on 64-67 day old Tianlu Ma hens

[0076]

[0077] It can be seen from Table 6 that the specific gravity of the concentrate of Example 6 is 1.30. Compared with the blank control group, the feed-to-meat ratio is 0.31 lower when 3 kg is added per ton of water, i.e. 0.3% is added, and the feed-to-meat ratio is reduced, and the effect of reducing the feed-to-meat ratio is relatively the best; while when 1 kg is added per ton of water, i.e. 0.1%, the feed-to-meat ratio is increased by 0.4 compared with the control group. The small amount of addition effectively saves the addition cost, but cannot effectively reduce the feed-to-meat ratio and reduce the feed cost; It shows that Example 5 is more appropriate to add 2 kg and less than 5 kg per ton of water at 0.1% < addition amount ≤ 0.3%.

[0078] From the experimental results in Table 1-6 above, we can see that:

[0079] 1. Compared with the blank control group, the concentrate prepared in each embodiment of the present scheme can reduce the feed-to-meat ratio to a certain extent after being used for broilers at different stages, and the effects obtained by adding different amounts of drinking water are different. The concentrate can achieve different degrees of reduction in feed-to-meat ratio by adding 1-5L per ton of water, that is, 0.1%-0.5%; to achieve a more ideal comprehensive effect, the concentrate is added at 2-4L per ton of water, that is, 0.2%-0.4%; to achieve a more ideal comprehensive effect of less input and high output, the concentrate is added at 2-3L per ton of water, that is, 0.2%-0.3%.

[0080] 2. The specific gravity of the concentrate has a certain relationship with the amount of additives in aquaculture. When the specific gravity is high, the amount of additives in aquaculture should be appropriately reduced, and when the specific gravity is low, the amount of additives in aquaculture should be appropriately increased.

[0081] 3. The higher the specific gravity value, the higher the content of various ingredients in the product. When a large amount of concentrated liquid with a higher concentration is added, the ideal input-output ratio cannot be achieved in breeding. Only by obtaining a more ideal specific gravity with an appropriate concentration ratio and concentration time can an ideal input-output ratio be achieved.

[0082] In summary, the concentrated solution obtained by the present invention can promote growth and improve the appearance of coat color when used as an additive for livestock and poultry. In addition, since the concentrated solution prepared by the present invention only needs to be used under free drinking water, it has high bioavailability, small addition amount, saves costs, does not require mixing, has low labor intensity, and will not cause adverse effects caused by mixing with feed. The above multiple characteristics can bring better economic benefits to the breeding industry.

[0083] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. An application of concentrated solution after extracting sweet glycosides in livestock and poultry breeding, characterized in that: The preparation process of the concentrated solution after the extraction of sweet glycosides comprises the following steps: S1. The mogroside aqueous solution passes through an adsorption resin column, and the chromatographic liquid discharged from the adsorption resin column is collected; S2. The chromatographic liquid is concentrated under vacuum to obtain a semi-finished concentrated liquid having a specific gravity of 1.12-1.39; the vacuum degree during the vacuum concentration is 0.06-0.08 MPa, the concentration temperature is ≤70°C, the concentration time is 90-120 min, and the chromatographic liquid and the concentrated liquid are concentrated under vacuum at a ratio of 30-40:1; S3. The semi-finished concentrated liquid is sterilized and cooled to obtain the concentrated liquid; The usage amount of the concentrated liquid is 0.1%-0.5% of the water mass.

2. The use of the concentrated solution after the extraction of sweet glycosides according to claim 1 in livestock and poultry breeding is characterized in that: In step S3, cooling is performed at room temperature.

3. The use of the concentrated solution after the extraction of sweet glycosides according to claim 1 in livestock and poultry breeding is characterized in that: The sterilization process in step S3 is high temperature sterilization at 108-112°C, and the flow rate of the semi-finished concentrated liquid is 0.8-1.0m 3 / h, 3-8 seconds instant sterilization.

4. The use of the concentrated solution after the extraction of sweet glycosides according to claim 1 in livestock and poultry breeding is characterized in that: In step S1, the pH value of the obtained chromatographic solution is 4.5-5.

5.

5. The use of the concentrated solution after the extraction of sweet glycosides according to claim 1 in livestock and poultry breeding, characterized in that: The step S2 comprises the following steps: the chromatography liquid and the concentrated liquid are concentrated under vacuum at a ratio of 33-38:1 to obtain a semi-finished concentrated liquid with a specific gravity of 1.18-1.

35.

6. The use of the concentrated solution after the extraction of sweet glycosides according to claim 1 in livestock and poultry breeding, characterized in that: The step S2 comprises the following steps: the chromatography liquid and the concentrated liquid are concentrated under vacuum at a ratio of 35:1 to obtain a semi-finished concentrated liquid with a specific gravity of 1.22-1.30.

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