A processing method for promoting germination and growth of oat seeds

Abstract

The present invention provides a treatment method for promoting the germination and growth of oat seeds, and relates to the technical field of oat seed treatment. The treatment method includes setting tea polyphenols, 2,3-butylene glycol, caffeine, and vitamin B12 as seed soaking agents, using seed soaking agents of different concentrations to soak oat seeds twice in an ice water bath with boosted pressure for a short time and once in a normal pressure with a long time, and then using a disinfectant to treat and sow. The present invention overcomes the deficiencies of the prior art, and sets seed soaking agents of different concentrations to pre-treat seeds under ice water bath boosted conditions, which can effectively promote the germination and subsequent growth of oat seeds and achieve the effect of planting.

Description

Technical Field

[0001] The invention relates to the technical field of oat seed treatment, and in particular to a treatment method for promoting the germination and growth of oat seeds. Background Art

[0002] Oats are annual herbaceous plants of the genus Avena in the Poaceae family. They are resistant to extreme environments such as barrenness, drought and severe cold, and are suitable for planting in high-cold areas and arid and semi-arid areas. They are widely planted as both food and feed crops worldwide. Oats are rich in vitamins, proteins, minerals and other substances necessary for the human body, and are one of the healthy and safe food sources. Therefore, more and more people are adding oats to their diets. Due to their high yield, good palatability and high nutritional value, oats have become a material for silage and hay preparation in grassland animal husbandry, which can solve the major problem of insufficient forage supply in winter and spring and promote the development of animal husbandry.

[0003] At present, my country's oat production cannot meet domestic demand. The import volume of oats occupies a dominant position in the world's total oat imports and shows an overall upward trend. Therefore, it is of great significance to increase my country's oat production.

[0004] Oat seeds have a high oil content, and the linoleic acid content accounts for 38.1%-52.0% of fatty acids, which makes them more susceptible to aging due to the rancidity of fat derivatives during storage. The end result is that the germination rate and subsequent growth efficiency of the seeds will be significantly reduced, which will cause a certain degree of oat production reduction. Therefore, on this basis, processing oat seeds to improve their germination rate and growth efficiency is an important research direction at this stage. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a treatment method for promoting the germination and growth of oat seeds. Seed soaking agents of different concentrations are set to pretreat the seeds under ice water bath pressure, which can effectively promote the germination and subsequent growth of the oat seeds and achieve the planting effect.

[0006] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] A method for promoting the germination and growth of oat seeds, the method comprising the following steps:

[0008] S1. Preparation of seed soaking agent: prepare seed soaking agent according to 100-120 mg / L of tea polyphenols, 40-60 mL / L of 2,3-butanediol, 10-20 mg / L of caffeine, 50-80 mg / L of vitamin B12, and the rest is water;

[0009] S2. Preliminary seed soaking treatment: dilute the above seed soaking agent with water by 2 times, then put in oat seeds, increase the pressure to 0.2-0.3 MPa in an ice water bath and let stand for 15-20 minutes, take out the preliminarily treated oat seeds for use;

[0010] S3, secondary seed soaking treatment: the oat seeds treated initially are placed in a seed soaking agent diluted 5 times with water, the pressure is increased to 0.4-0.5 MPa under ice water bath conditions, and the pressure is left to stand for 8-10 minutes, and then the oat seeds treated twice are taken out for use;

[0011] S4, three-time seed soaking treatment: place the above-mentioned twice-treated oat seeds in a seed soaking agent diluted 10 times with water, soak for 5-8 hours at room temperature and pressure, and then soak for 5-10 minutes at a water bath temperature of 30-45°C to obtain three-time-treated oat seeds for use;

[0012] S5, disinfection treatment: placing the oat seeds treated three times in a disinfectant, taking them out after stirring, and allowing the surface to dry naturally, thereby obtaining the disinfected oat seeds for use;

[0013] S6. Sowing: placing the disinfected oat seeds on a planting site for sowing.

[0014] Preferably, the method for preparing the seed soaking agent in step S1 is to add the corresponding weights of the ingredients into water, and disperse them by ultrasonic vibration at 400-600W for 10-15 minutes.

[0015] Preferably, the oat seeds are rinsed once with clean water before the secondary seed soaking treatment and the tertiary seed soaking treatment.

[0016] Preferably, the interval time between the initial seed soaking treatment, the secondary seed soaking treatment and the tertiary seed soaking treatment is ≤5 min.

[0017] Preferably, the pressurized environment of the primary seed soaking treatment and the secondary seed soaking treatment are both carried out under nitrogen protection.

[0018] Preferably, the water bath temperature in step S4 is 35°C.

[0019] Preferably, the disinfectant in step S5 is a water diluent of any one or more of the following drugs: 35% imidacloprid suspension, 10% difenoconazole water dispersible granules, 35% thiamethoxam suspension, and 50% fludioxonil wettable powder.

[0020] Preferably, the stirring speed in step S5 is 80-120 r / min, and the stirring time is 3-5 min.

[0021] Preferably, the sowing in step S6 is carried out within 12 hours after the disinfection treatment.

[0022] The present invention provides a treatment method for promoting the germination and growth of oat seeds, which has the following advantages over the prior art:

[0023] The present invention adopts tea polyphenols, 2,3-butanediol, caffeine and vitamin B12 as seed soaking ingredients, wherein tea polyphenols, 2,3-butanediol and vitamin B12 can effectively enhance the vitality of seeds, and although caffeine can inhibit the germination activity of seeds to a certain extent, by adding a small amount of caffeine and subsequent soaking treatment with ice water bath and variable pressure, the influence of seed aging can be reduced to a certain extent, and the growth of subsequent oat seeds can be promoted, thereby providing a good foundation for large-scale production increase of oats. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the germination and growth of oat seeds treated by the treatment methods of Comparative Example 3, Comparative Example 2 and Example 1 of the present invention, and a germination experiment was conducted for 3 days.

[0025] Figure 2 This is a schematic diagram of the sowing of oat seeds in the sowing experiment of the present invention;

[0026] Figure 3 A schematic diagram of the growth of oat seeds treated by the treatment methods of Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 of the present invention 6 days after sowing;

[0027] Figure 4 This is a schematic diagram of the growth of oat seeds treated by the treatment method of Comparative Example 4 of the present invention and untreated oat seeds 6 days after sowing. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Embodiment 1:

[0029] Oat seed treatment:

[0030] (1) Weigh the ingredients according to the following content and add them into water. Disperse them under 400W ultrasonic vibration for 15 minutes to prepare the seed soaking agent: tea polyphenols 100 mg / L, 2,3-butanediol 40 mL / L, caffeine 10 mg / L, vitamin B12 50 mg / L;

[0031] (2) Dilute the above seed soaking agent with water by 2 times, then mix it with oat seeds and submerge them, increase the pressure to 0.2 MPa in an ice water bath and under nitrogen protection, and let it stand for 20 minutes, then take out the preliminarily treated oat seeds for use;

[0032] (3) After washing the oat seeds treated initially with water, immediately place them in a seed soaking agent diluted 5 times with water, increase the pressure to 0.4 MPa in an ice water bath and under nitrogen protection, and let it stand for 10 minutes. Then take them out to obtain the oat seeds treated twice for use;

[0033] (4) After washing the oat seeds treated twice with clean water, immediately place them in a seed soaking agent diluted 10 times with water, soak them for 5 hours at room temperature and pressure, and then soak them in a water bath at 35°C for 5 minutes to obtain oat seeds treated three times for use;

[0034] (5) Disinfection treatment: Place the oat seeds treated three times in a solution diluted 100 times with 35% imidacloprid suspension, stir at 80 r / min for 5 min, then take out and allow the surface to dry naturally. The disinfected oat seeds are ready for use. Embodiment 2:

[0035] Oat seed treatment:

[0036] (1) Preparation of seed soaking agent: prepare the seed soaking agent according to 120 mg / L tea polyphenols, 60 mL / L 2,3-butanediol, 20 mg / L caffeine, 80 mg / L vitamin B12, and the rest is water;

[0037] S2, preliminary seed soaking treatment: dilute the above seed soaking agent with water by 2 times, then put oat seeds in, increase the pressure to 0.3 MPa under ice water bath and nitrogen protection, and let it stand for 15 minutes, take out the preliminary treated oat seeds for use;

[0038] S3, secondary seed soaking treatment: the oat seeds treated initially are placed in a seed soaking agent diluted 5 times with water, the pressure is increased to 0.5 MPa under an ice water bath and nitrogen protection, and the mixture is allowed to stand for 8 minutes, and then taken out to obtain the secondary treated oat seeds for use;

[0039] S4, three-time seed soaking treatment: Place the above-mentioned twice-treated oat seeds in a seed soaking agent diluted 10 times with water, soak for 8 hours at room temperature and pressure, and then soak for 10 minutes at a water bath temperature of 35°C to obtain three-time-treated oat seeds for use;

[0040] S5. Disinfection treatment: Place the oat seeds treated three times in a solution of 10% difenoconazole water dispersible granules diluted 100 times, stir at 120 r / min for 3 minutes, then take out and allow the surface to dry naturally to obtain disinfected oat seeds.

[0041] Comparative Example 1:

[0042] Oat seed treatment:

[0043] (1) Weigh the ingredients according to the following content and add them into water. Disperse them under 400W ultrasonic vibration for 15 minutes to prepare the seed soaking agent: tea polyphenols 100 mg / L, 2,3-butanediol 40 mL / L, caffeine 10 mg / L, vitamin B12 50 mg / L;

[0044] (2) diluting the above seed soaking agent by 10 times with water, soaking the seeds at room temperature and pressure for 5 hours, and then soaking them in a water bath at 35°C for 5 minutes to obtain pretreated oat seeds for use;

[0045] (3) Disinfection: Place the pretreated oat seeds in a solution diluted 100 times of 35% imidacloprid suspension, stir at 80 r / min for 5 min, then take out and allow the surface to dry naturally. The disinfected oat seeds are ready for use.

[0046] Comparative Example 2:

[0047] Oat seed treatment:

[0048] (1) Weigh the ingredients according to the following content and add them into water. Disperse them under 400W ultrasonic vibration for 15 minutes. Prepare the seed soaking agent: tea polyphenols 100 mg / L, 2,3-butanediol 40 mL / L, vitamin B12 50 mg / L;

[0049] (2) Dilute the above seed soaking agent with water by 2 times, then mix it with oat seeds and submerge them, increase the pressure to 0.2 MPa in an ice water bath and under nitrogen protection, and let it stand for 20 minutes, then take out the preliminarily treated oat seeds for use;

[0050] (3) After washing the oat seeds treated initially with water, immediately place them in a seed soaking agent diluted 5 times with water, increase the pressure to 0.4 MPa in an ice water bath and under nitrogen protection, and let it stand for 10 minutes. Then take them out to obtain the oat seeds treated twice for use;

[0051] (4) After washing the oat seeds treated twice with clean water, immediately place them in a seed soaking agent diluted 10 times with water, soak them for 5 hours at room temperature and pressure, and then soak them in a water bath at 35°C for 5 minutes to obtain oat seeds treated three times for use;

[0052] (5) Disinfection treatment: Place the oat seeds treated three times in a solution diluted 100 times with 35% imidacloprid suspension, stir at 80 r / min for 5 min, then take out and allow the surface to dry naturally. The disinfected oat seeds are ready for use.

[0053] Comparative Example 3:

[0054] Oat seed treatment:

[0055] (1) Weigh the ingredients according to the following content and add them into water. Disperse them under 400W ultrasonic vibration for 15 minutes to prepare the seed soaking agent: tea polyphenols 100 mg / L, 2,3-butanediol 40 mL / L, caffeine 10 mg / L, vitamin B12 50 mg / L;

[0056] (2) Dilute the above seed soaking agent with water by 2 times, then mix it with oat seeds and submerge them, increase the pressure to 0.2 MPa in an ice water bath and under nitrogen protection, and let it stand for 20 minutes, then take out the preliminarily treated oat seeds for use;

[0057] (3) After washing the oat seeds treated initially with water, immediately place them in a seed soaking agent diluted 2 times with water, increase the pressure to 0.4 MPa in an ice water bath and under nitrogen protection, and let it stand for 10 minutes. Then take them out to obtain the oat seeds treated twice for use;

[0058] (4) After washing the oat seeds treated twice with clean water, immediately place them in a seed soaking agent diluted 2 times with water, soak them at room temperature and pressure for 5 hours, and then soak them in a water bath at 35°C for 5 minutes to obtain oat seeds treated three times for use;

[0059] (5) Disinfection treatment: Place the oat seeds treated three times in a solution diluted 100 times with 35% imidacloprid suspension, stir at 80 r / min for 5 min, then take out and allow the surface to dry naturally. The disinfected oat seeds are ready for use.

[0060] Comparative Example 4:

[0061] Oat seed treatment:

[0062] (1) Weigh the ingredients according to the following content and add them into water. Disperse them under 400W ultrasonic vibration for 15 minutes to prepare the seed soaking agent: tea polyphenols 100 mg / L, 2,3-butanediol 40 mL / L, caffeine 10 mg / L, vitamin B12 50 mg / L;

[0063] (2) Dilute the above seed soaking agent with water by 5 times, then mix it with oat seeds and submerge them, increase the pressure to 0.2 MPa in an ice water bath and under nitrogen protection, and let it stand for 20 minutes. Take out the preliminarily treated oat seeds for use;

[0064] (3) After washing the oat seeds treated initially with water, immediately place them in a seed soaking agent diluted 5 times with water, increase the pressure to 0.4 MPa in an ice water bath and under nitrogen protection, and let it stand for 10 minutes. Then take them out to obtain the oat seeds treated twice for use;

[0065] (4) After washing the oat seeds treated twice with clean water, immediately place them in a seed soaking agent diluted 5 times with water, soak them for 5 hours at room temperature and pressure, and then soak them in a water bath at 35°C for 5 minutes to obtain oat seeds treated three times for use;

[0066] (5) Disinfection treatment: Place the oat seeds treated three times in a solution diluted 100 times with 35% imidacloprid suspension, stir at 80 r / min for 5 min, then take out and allow the surface to dry naturally. The disinfected oat seeds are ready for use.

[0067] Detection:

[0068] The same batch of black rose oat seeds were selected as experimental materials, and were processed by the methods of the above-mentioned Examples 1-2 and Comparative Examples 1-4, and then the following experiments were carried out:

[0069] 1. Seed germination experiment:

[0070] A double layer of filter paper soaked in distilled water was laid in a culture dish, 25 treated oat seeds were sprinkled in each culture dish (4 culture dishes were set for each group of pre-treated seeds), and the dish was placed in a 20°C incubator for germination test. The germination rate of oat seeds was recorded daily for 1d, 2d, and 5d in each group. The specific results are shown in Table 1 below:

[0071] Table 1

[0072]

[0073] As can be seen from Table 1 above, the germination rate of oat seeds can be significantly improved by treating oat seeds in the manner of Example 1 and Example 2. In Comparative Example 1, the pressurized ice water bath is not used for soaking seeds, and the addition of caffeine will significantly inhibit the germination of seeds. In Comparative Example 2, the efficiency of promoting oat seed germination in the early stage by reducing the addition of caffeine will be higher, but the subsequent germination effect is significantly weakened. In Comparative Examples 3 and 4, the concentration of the soaking solution for soaking seeds is adjusted, which also reduces the germination effect of oat seeds to a certain extent.

[0074] 2. Seeding experiment:

[0075] Garden soil is used as the matrix. The specific properties of the garden soil are shown in Table 2 below:

[0076] Table 2

[0077]

[0078] The garden soil was filled into the seedling tray, and then the oat seeds after treatment in each group were sown in the seedling tray within 12 hours for routine management. The emergence rate was recorded 5d, 8d, and 10d after sowing. The specific results are shown in Table 3 below:

[0079] Table 3

[0080]

[0081] The height of each group of oat seedlings was recorded at 10d and 15d after sowing (five oat seedlings were randomly selected from each group and the average value was calculated). The specific results are shown in Table 4 below:

[0082] Table 4

[0083]

[0084] It can be seen from the above table that the seeds treated in Example 1 and Example 2 have better growth performance.

[0085] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A treatment method for promoting the germination and growth of oat seeds, It is characterized in that The processing method comprises the following steps: S1. Preparation of seed soaking agent: prepare seed soaking agent according to 100-120 mg / L of tea polyphenols, 40-60 mL / L of 2,3-butanediol, 10-20 mg / L of caffeine, 50-80 mg / L of vitamin B12, and the rest is water; S2. Preliminary seed soaking treatment: dilute the above seed soaking agent with water by 2 times, then put in oat seeds, increase the pressure to 0.2-0.3 MPa in an ice water bath and let stand for 15-20 minutes, take out the preliminarily treated oat seeds for use; S3, secondary seed soaking treatment: the oat seeds treated initially are placed in a seed soaking agent diluted 5 times with water, the pressure is increased to 0.4-0.5 MPa under ice water bath conditions, and the pressure is left to stand for 8-10 minutes, and then the oat seeds treated twice are taken out for use; S4, three-time seed soaking treatment: place the above-mentioned twice-treated oat seeds in a seed soaking agent diluted 10 times with water, soak for 5-8 hours at room temperature and pressure, and then soak for 5-10 minutes at a water bath temperature of 30-45°C to obtain three-time-treated oat seeds for use; S5, disinfection treatment: placing the oat seeds treated three times in a disinfectant, taking them out after stirring, and allowing the surface to dry naturally, thereby obtaining the disinfected oat seeds for use; S6. Sowing: placing the disinfected oat seeds on the planting site for sowing.

2. The processing method according to claim 1, Features: The method for preparing the seed soaking agent in step S1 is to weigh the corresponding weight of each component and add it into water, and disperse it by ultrasonic vibration at 400-600W for 10-15 minutes.

3. The processing method according to claim 1, Features: Before the secondary seed soaking treatment and the tertiary seed soaking treatment, the oat seeds are first rinsed once with clean water.

4. The processing method according to claim 1, Features: The interval time between the initial seed soaking treatment, the secondary seed soaking treatment and the tertiary seed soaking treatment is ≤5min.

5. The processing method according to claim 1, Features: The pressurized environment of the primary seed soaking treatment and the secondary seed soaking treatment are both carried out under nitrogen protection.

6. The processing method according to claim 1, Features: The water bath temperature in step S4 is 35°C.

7. The processing method according to claim 1, Features: The disinfectant in step S5 is a water diluent of any one or more of the following drugs: 35% imidacloprid suspension, 10% difenoconazole water dispersible granules, 35% thiamethoxam suspension, and 50% fludioxonil wettable powder.

8. The processing method according to claim 1, Features: The stirring speed in step S5 is 80-120 r / min, and the stirring time is 3-5 min.

9. The processing method according to claim 1, Features: The sowing time in step S6 is carried out within 12 hours after the disinfection treatment.

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