A preparation device for glucose oxidase

Abstract

The present invention relates to the field of glucose oxidase preparation, and discloses a glucose oxidase preparation device, including a salting-out chamber, wherein a mixed aluminum cylinder is movably mounted on the inner upper side of the salting-out chamber through a bearing seat, a central axis is movably mounted in the inner middle of the salting-out chamber, a stirring rod is fixedly connected to both sides of the middle of the central axis, a magnetic rod is fixedly mounted on both sides of the inner side of the salting-out chamber, a feeding chamber is fixedly connected to both sides of the inner side of the shell, and the bottom of the feeding chamber is connected to the inner side of the mixed aluminum cylinder. The present invention drives the stirring rod to rotate clockwise through the central axis, and the mixed aluminum cylinder rotates counterclockwise at the same time, and the two cooperate to fully mix the crude enzyme solution and the salting-out material, thereby improving the mixing effect, and utilizing the magnetic rods on both sides of the inner side of the salting-out chamber to generate an induced current and an electric field when the mixed aluminum cylinder rotates, so that the charged glucose oxidase molecules are accelerated to interact and precipitate with the salting-out agent under the action of the electric field, thereby improving the salting-out efficiency.

Description

Technical Field

[0001] The invention relates to the field of glucose oxidase preparation, in particular to a glucose oxidase preparation device. Background Art

[0002] In the field of biotechnology, glucose oxidase, as an important industrial enzyme preparation, is widely used in food, medicine, chemical industry and other industries. Its main function is to catalyze the oxidation of glucose into gluconic acid and hydrogen peroxide.

[0003] Traditional methods for preparing glucose oxidase usually involve multiple complex steps, among which the salting-out process is a key step. In the salting-out process, the crude enzyme solution needs to be mixed with a salting-out agent to cause the glucose oxidase to precipitate. However, the existing salting-out device has some shortcomings in practical applications.

[0004] On the one hand, it is difficult to accurately control the amount of salting-out agent added in traditional salting-out devices, which may lead to unstable salting-out effect and affect the yield and quality of glucose oxidase. On the other hand, during the salting-out process, the mixing effect of crude enzyme solution and salting-out agent is often not ideal, which will prolong the reaction time and reduce production efficiency.

[0005] In addition, the existing salting-out device also needs to be improved in terms of salting-out efficiency. In order to overcome these problems, researchers are committed to developing a new type of glucose oxidase preparation device to achieve precise control of the amount of salting-out agent added, improve the mixing effect of crude enzyme solution and salting-out agent, and improve salting-out efficiency. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention provides a preparation device for glucose oxidase, which solves the problem that it is difficult for traditional salting-out devices to accurately control the amount of salting-out agent added, which may lead to unstable salting-out effect and the mixing effect of crude enzyme solution and salting-out agent during the salting-out process is often not ideal, which will prolong the reaction time and reduce production efficiency.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a preparation device for glucose oxidase, comprising a salting-out chamber, a mixed aluminum cylinder is movably mounted on the inner upper side of the salting-out chamber through a bearing seat, a central axis rod is movably mounted in the inner middle of the salting-out chamber, stirring rods are fixedly connected to both sides of the middle of the central axis rod, magnetic rods are fixedly mounted on both sides of the inner part of the salting-out chamber, a shell is fixedly connected to the middle of the top of the salting-out chamber, a feeding chamber is fixedly connected to both sides of the inner part of the shell, and the bottom ends of the feeding chambers are connected to the inner part of the mixed aluminum cylinder, and a movable seat is movably mounted in the inner middle of the feeding chamber. A feeding channel is opened in the middle of the movable seat, and a stopper is movably installed on the inner top of the feeding chamber. The bottom end of the stopper is fixedly connected with a long rod, and the end of the long rod penetrates the interior of the feeding channel and extends to the interior of the mixed aluminum cylinder. A sealing block is fixedly connected to the outer diameter of the long rod near the bottom of the feeding channel. A cylinder body is fixedly installed on the upper outer diameter of the central axis rod, and an inclined oblique bearing seat is movably provided on the outer diameter of the cylinder body. Both ends of the oblique bearing seat are fixedly connected with a rocker arm, and a connecting rod is movably installed at the end of the rocker arm, and the end of the connecting rod is movably installed at the bottom of the long rod on the corresponding side.

[0008] Preferably, a three-phase asynchronous motor is fixedly installed on one side of the inner bottom of the salting-out chamber, a driving bevel gear is fixedly installed on the driving end of the three-phase asynchronous motor, a first driven bevel gear is fixedly installed on the lower outer diameter of the central axis, a connecting ring is fixedly connected to the bottom end of the mixed aluminum cylinder, a second driven bevel gear is fixedly connected to the bottom end of the connecting ring, and one side of the first driven bevel gear and the second driven bevel gear are both meshed and connected with the outer end of the driving bevel gear.

[0009] Preferably, a fixed seat is fixedly installed on the inner bottom of the unloading chamber, guide rods are fixedly connected to the inner sides of the fixed seat, guide grooves are opened on the inner sides of the movable seat, and the ends of the guide rods are movably arranged inside the guide grooves on the corresponding sides.

[0010] Preferably, an external thread is provided on the middle outer diameter of the movable seat, a retaining ring is movably installed on the lower outer diameter of the discharge chamber, an internal thread is provided on the inner end of the retaining ring, and the inner end of the internal thread is connected to the outer end thread of the retaining ring.

[0011] Preferably, the outer ends of the retaining rings are fixedly connected to a worm wheel, a worm is movably installed inside the housing and both sides of the worm are meshed and connected to the inner side of the worm wheel, and one end of the worm extends to the outside of the housing and is fixedly installed with a knob.

[0012] Preferably, a silo is fixedly connected to the top of the shell, and two discharge branch pipes are fixedly connected to the bottom of the silo, and the ends of the discharge branch pipes extend to the inner upper side of the discharge chamber on the corresponding side.

[0013] Preferably, a detector is fixedly installed on the other side of the inner bottom of the salting-out chamber, a detection tube is fixedly installed on the top of the detector, a leakage tube is fixedly connected to one side of the bottom of the mixed aluminum cylinder, a pressure ring is connected to one side of the bottom of the mixed aluminum cylinder near the outer side of the leakage tube through a reset spring, a push rod is fixedly installed in the middle of the pressure ring, the top of the push rod extends to the top of the leakage tube and is fixedly installed with a sealing plug.

[0014] Preferably, a door is provided on the top front side of the salting-out chamber, an observation window is fixedly connected to the upper front side of the salting-out chamber, and a controller is fixedly installed on the lower front side of the salting-out chamber.

[0015] Working principle: first open the door, pour a certain amount of crude enzyme liquid raw material into the mixing aluminum cylinder and then close the door, pour the required salting-out material such as ammonium sulfate solid particles into the silo, and the salting-out material will enter the two unloading chambers along the unloading branch pipe, and the worm will rotate by rotating the knob. The rotating worm and the worm gear will mesh with each other to drive the retaining rings on the outer diameters of the two unloading chambers to rotate. The rotating retaining ring will drive the inner thread on the inside to mesh with the outer thread on the outer diameter of the movable seat, and the movable seat will be lifted and lowered by the limiting effect of the guide rod and the guide groove. The lifting and lowering of the movable seat can change the size of the space on the upper side of the unloading chamber, thereby changing the amount of salting-out material entering the unloading chamber each time, which is convenient for precise control of the unloading amount of the salting-out material and completes the adjustment. After that, start the three-phase asynchronous motor, and drive the main shaft to rotate through the three-phase asynchronous motor to drive the active bevel gear to rotate. The rotating active bevel gear will mesh with the first driven bevel gear and the second driven bevel gear. When the first driven bevel gear rotates, it will drive the middle shaft to rotate clockwise. When the middle shaft rotates, it will drive the stirring rod to rotate with it, and the raw materials in the mixed aluminum cylinder will be stirred clockwise, while the second driven bevel gear will rotate counterclockwise, thereby driving the connecting ring and the mixed aluminum cylinder to rotate counterclockwise. The clockwise rotating stirring rod cooperates with the counterclockwise rotating mixing aluminum cylinder to complete the mixing of the crude enzyme solution and the salting out product, thereby improving the mixing effect. When the middle shaft rotates, it will also drive the top cylinder body to rotate. Due to the existence of the inclined bearing seat and the center axis of the cylinder body, When the cylinder body rotates, the balls are driven to move in the ball grooves, thereby driving the two ends of the inclined bearing seat to swing up and down, and cooperating with the rocker arm and the connecting rod to drive the long rods on both sides to move up and down alternately. When the long rod moves down, the block and the blocking block are driven to move down, and the downward moving block will close the connection between the discharge branch pipe and the discharge chamber to prevent the salt precipitation from entering the discharge chamber, and when the blocking block moves down, the bottom of the discharge channel will be opened, and the salt precipitation in the discharge chamber will enter the mixed aluminum cylinder along the discharge channel, and when the long rod moves up, the blocking block will block the discharge channel again, and the block will also release the blocking of the discharge branch pipe, and the salt precipitation will enter the discharge chamber again. In the chamber, the two long rods are used to work alternately up and down in a reciprocating manner to realize the continuous feeding of the salting out material, so as to facilitate the mixing with the crude enzyme solution in the mixing aluminum cylinder. In addition, since magnetic bars are arranged on both sides of the interior of the salting out chamber, when the mixing aluminum cylinder rotates, the magnetic flux of the mixing aluminum cylinder will change, thereby generating an induced current and forming an electric field, so that the charged glucose oxidase molecules in the crude enzyme solution move toward the specific electrode under the action of the electric field, accelerating the interaction and precipitation with the salting out agent, and improving the salting out efficiency. At the same time, when the mixing aluminum cylinder rotates, it will also drive the pressure ring at the bottom to do circular motion. When the bottom of the moving pressure ring contacts the top of the detection tube, it will be pushed upward for a short distance, thereby driving the ejector rod and the sealing plug to move upward, and opening the top opening of the leakage tube.The mixed liquid flows along the leaking tube into the detection tube and finally into the detector. The detector is used to detect the parameters of the mixed liquid. As the mixing aluminum cylinder continues to rotate, the pressure ring and the detection tube are separated. Under the action of the reset spring, the pressure ring is reset, driving the ejector rod and the sealing plug to reset, preventing the mixed liquid from leaking out. The cycle is repeated, and the detector can detect the mixed liquid parameters in real time.

[0016] The present invention provides a device for preparing glucose oxidase, which has the following beneficial effects:

[0017] 1. The present invention drives the stirring rod to rotate clockwise through the central axis rod, and the mixing aluminum cylinder rotates counterclockwise at the same time. The cooperation of the two can fully mix the crude enzyme solution and the salting-out product, thereby improving the mixing effect. At the same time, the rotation of the central axis rod drives the cylinder body, the oblique bearing seat, the swing rod and the connecting rod to move, so that the long rods on both sides move up and down alternately, thereby realizing the continuous discharge of the salting-out product, which is convenient for mixing with the crude enzyme solution. In addition, the magnetic bars on both sides of the salting-out chamber generate induced current and electric field when the mixing aluminum cylinder rotates, so that the charged glucose oxidase molecules are accelerated to interact and precipitate with the salting-out agent under the action of the electric field, thereby improving the salting-out efficiency.

[0018] 2. The present invention drives the worm, worm wheel and snap ring to rotate by rotating the knob. The snap ring and the movable seat are threadedly transmitted. With the limit of the guide rod and the guide groove, the lifting and lowering of the movable seat can be accurately adjusted, thereby changing the size of the internal space of the discharge chamber and realizing accurate control of the discharge amount of the salt precipitation material.

[0019] 3. The rotation of the mixing aluminum cylinder of the present invention drives the pressure ring to make a circular motion. When the pressure ring contacts the detection tube, the mixed liquid can enter the detector for real-time parameter detection. After the detection is completed, the sealing plug is reset under the action of the reset spring to prevent the mixed liquid from leaking out. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A perspective view of the present invention;

[0021] Figure 2 It is a schematic diagram of the internal structure of the present invention;

[0022] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0023] Figure 4 for Figure 2 Enlarged view of point B in the middle;

[0024] Figure 5 for Figure 2 Enlarged view of point C in the middle;

[0025] Figure 6 It is a schematic diagram of the internal structure of the feeding chamber in the present invention.

[0026] Among them, 1. salt precipitation chamber; 2. mixing aluminum cylinder; 3. three-phase asynchronous motor; 4. driving bevel gear; 5. middle shaft; 6. first driven bevel gear; 7. connecting ring; 8. second driven bevel gear; 9. stirring rod; 10. magnetic rod; 11. shell; 12. unloading chamber; 13. movable seat; 14. unloading channel; 15. fixed seat; 16. guide rod; 17. guide groove; 18. external thread; 19. snap ring; 20. internal thread; 21. Worm gear; 22. Block; 23. Long rod; 24. Block; 25. Worm; 26. Knob; 27. Bin; 28. Feed branch pipe; 29. ​​Cylinder; 30. Oblique bearing seat; 31. Rocker arm; 32. Connecting rod; 33. Detector; 34. Detection tube; 35. Leakage tube; 36. Reset spring; 37. Press ring; 38. Push rod; 39. Sealing plug; 40. Bin door; 41. Observation window; 42. Controller. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings of the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example:

[0029] Please see attached Figure 1 -Attached Figure 6 The present invention provides a preparation device for glucose oxidase, such as Figure 1As shown, it includes a salting-out chamber 1, and a mixed aluminum cylinder 2 made of aluminum is movably installed on the upper side of the interior of the salting-out chamber 1 through a bearing seat. When the mixed aluminum cylinder 2 rotates, the magnetic flux of the mixed aluminum cylinder 2 will change, thereby generating an induced current and forming an electric field, so that the charged glucose oxidase molecules in the crude enzyme solution move toward a specific electrode under the action of the electric field, accelerating the interaction and precipitation with the salting-out agent, and improving the salting-out efficiency. At the same time, aluminum will not react with the salting-out material and the crude enzyme solution. A central axis rod 5 is movably installed in the middle of the salting-out chamber 1. The upper and lower ends of the central axis rod 5 are both installed on the inner wall of the salting-out chamber 1 through a bearing seat, and the middle part passes through the bottom of the mixed aluminum cylinder 2. The central axis rod 5 Stirring rods 9 are fixedly connected to both sides of the middle of the rod 5, which are the main structure for stirring and mixing. Magnetic bars 10 are fixedly installed on both sides of the interior of the salting-out chamber 1. A shell 11 is fixedly connected to the middle of the top of the salting-out chamber 1, which is installed for fixed protection. A feeding chamber 12 is fixedly connected to both sides of the interior of the shell 11, and the bottom end of the feeding chamber 12 is connected to the interior of the mixing aluminum cylinder 2, which is convenient for feeding. A movable seat 13 is movably installed in the middle of the feeding chamber 12, and the movable seat 13 can move up and down in the feeding chamber 12, so as to change the size of the upper space of the feeding chamber 12. A feeding channel 14 is opened in the middle of the movable seat 13, which is the main channel for feeding. The inner top of the chamber 12 is movably installed with a block 22. When the block 22 moves down, the connection between the feed branch pipe 28 and the feed chamber 12 is closed to prevent the salt precipitation from entering the feed chamber 12. The bottom end of the block 22 is fixedly connected with a long rod 23, and the end of the long rod 23 passes through the inside of the feed channel 14 and extends to the inside of the mixed aluminum cylinder 2. The movement of the long rod 23 drives the block 22 to move. The outer diameter of the long rod 23 is fixedly connected with a blocking block 24 near the bottom of the feed channel 14. When the blocking block 24 moves down, the bottom of the feed channel 14 is opened, and the salt precipitation in the feed chamber 12 will enter the mixed aluminum cylinder 2 along the feed channel 14. A cylinder body 29 is fixedly installed on the outer diameter of the upper side of the middle axis rod 5, and an inclined oblique bearing seat 30 is movably arranged on the outer diameter of the cylinder body 29. Both ends of the oblique bearing seat 30 are fixedly connected with a swing rod 31, and the end of the swing rod 31 is movably installed with a connecting rod 32. The end of the connecting rod 32 is movably installed at the bottom of the corresponding side long rod 23. An inclined ball groove is provided on the outer diameter of the cylinder body 29, and the inner ring ball of the oblique bearing seat 30 is arranged in the ball groove. When the cylinder body 29 rotates, it will drive the ball to move in the ball groove, thereby driving the two ends of the oblique bearing seat 30 to swing up and down, and cooperate with the action of the swing rod 31 and the connecting rod 32 to drive the long rods 23 on both sides to move up and down alternately.

[0030] Specifically, when the middle shaft rod 5 rotates, it will also drive the cylinder body 29 on the top to rotate. Since there is a certain angle between the inclined bearing seat 30 and the central axis of the cylinder body 29, an inclined ball groove is provided on the outer diameter of the cylinder body 29, and the inner ring ball of the inclined bearing seat 30 is arranged in the ball groove. When the cylinder body 29 rotates, it will drive the ball to move in the ball groove, thereby driving the two ends of the inclined bearing seat 30 to swing up and down, and cooperate with the action of the swing rod 31 and the connecting rod 32 to drive the long rods 23 on both sides to move up and down alternately. When the long rod 23 moves downward, it will drive the block 22 and the blocking block 24 to move downward, and the downward moving block 22 will push the feeding branch pipe The connection between 28 and the discharge chamber 12 is closed to prevent the salted-out material from entering the discharge chamber 12. When the blocking block 24 moves downward, the bottom of the discharge channel 14 will be opened, and the salted-out material in the discharge chamber 12 will enter the mixed aluminum cylinder 2 along the discharge channel 14. When the long rod 23 moves upward, the blocking block 24 will block the discharge channel 14 again, and the block 22 will also release the obstruction of the discharge branch pipe 28, and the salted-out material will enter the discharge chamber 12 again. The cycle is repeated, and the two long rods 23 are alternately moved up and down to realize the continuous discharge of the salted-out material, which is convenient for mixing with the crude enzyme solution in the mixed aluminum cylinder 2.

[0031] In this embodiment, a three-phase asynchronous motor 3 is fixedly installed on one side of the inner bottom of the salting-out chamber 1, a driving bevel gear 4 is fixedly installed on the driving end of the three-phase asynchronous motor 3, a first driven bevel gear 6 is fixedly installed on the lower outer diameter of the middle shaft rod 5, a connecting ring 7 is fixedly connected to the bottom end of the mixed aluminum cylinder 2, a second driven bevel gear 8 is fixedly connected to the bottom end of the connecting ring 7, one side of the first driven bevel gear 6 and the second driven bevel gear 8 are both meshed and connected with the outer end of the driving bevel gear 4, and the main shaft is driven by the three-phase asynchronous motor 3 to rotate and drive the driving bevel gear 4 to rotate, the rotating driving bevel gear 4 will mesh with the first driven bevel gear 6 and the second driven bevel gear 8, when the first driven bevel gear 6 rotates, it will drive the middle shaft rod 5 to rotate clockwise, and the second driven bevel gear 8 will rotate counterclockwise, thereby driving the connecting ring 7 and the mixed aluminum cylinder 2 to rotate counterclockwise.

[0032] Specifically, the three-phase asynchronous motor 3 is started, and the main shaft is driven by the three-phase asynchronous motor 3 to rotate and drive the active bevel gear 4 to rotate. The rotating active bevel gear 4 will mesh with the first driven bevel gear 6 and the second driven bevel gear 8. When the first driven bevel gear 6 rotates, it will drive the central axis rod 5 to rotate clockwise. When the central axis rod 5 rotates, it will drive the stirring rod 9 to rotate accordingly, and the raw materials in the mixed aluminum cylinder 2 will be stirred clockwise, while the second driven bevel gear 8 will rotate counterclockwise, thereby driving the connecting ring 7 and the mixed aluminum cylinder 2 to rotate counterclockwise. The clockwise rotating stirring rod 9 cooperates with the counterclockwise rotating mixed aluminum cylinder 2 to complete the mixing of the crude enzyme solution and the salting out product, thereby improving the mixing effect. In addition, since magnetic bars 10 are provided on both sides of the interior of the salting out chamber 1, when the mixed aluminum cylinder 2 rotates, the magnetic flux of the mixed aluminum cylinder 2 will change, thereby generating an induced current and forming an electric field, so that the charged glucose oxidase molecules in the crude enzyme solution move toward specific electrodes under the action of the electric field, accelerating the interaction and precipitation with the salting out agent, thereby improving the salting out efficiency.

[0033] Furthermore, a fixed seat 15 is fixedly installed on the inner bottom of the unloading chamber 12 for connecting and fixing. The middle part of the fixed seat 15 is also hollow, and the side walls are attached to the inner walls of the unloading channel 14. Guide rods 16 are fixedly connected on both sides of the interior of the fixed seat 15, and guide grooves 17 are opened on both sides of the interior of the movable seat 13. The ends of the guide rods 16 are movably arranged inside the corresponding side guide grooves 17 to improve the stability of the movable seat 13 when moving.

[0034] Furthermore, an external thread 18 is provided on the middle outer diameter of the movable seat 13, and a retaining ring 19 is movably installed on the lower outer diameter of the discharge chamber 12. The inner end of the retaining ring 19 is provided with an internal thread 20, and the inner end of the internal thread 20 is threadedly connected to the outer end of the retaining ring 19. The rotating retaining ring 19 will drive the inner internal thread 20 to engage with the external thread 18 on the outer diameter of the movable seat 13 for transmission, and the movable seat 13 is driven to rise and fall by utilizing the limiting effect of the guide rod 16 and the guide groove 17. By lifting and lowering the movable seat 13, the size of the space on the upper side of the discharge chamber 12 can be changed, thereby changing the amount of salting out material entering the discharge chamber 12 each time.

[0035] Furthermore, the outer ends of the retaining rings 19 are fixedly connected to the worm gear 21, a worm 25 is movably installed inside the housing 11 and both sides of the worm 25 are meshingly connected to the inner sides of the worm gear 21, one end of the worm 25 extends to the outside of the housing 11 and is fixedly installed with a knob 26, and the worm 25 is driven to rotate by rotating the knob 26, and the rotating worm 25 is meshingly transmitted with the worm gear 21, driving the retaining rings 19 on the outer diameters of the two discharge chambers 12 to rotate.

[0036] Furthermore, a silo 27 is fixedly connected to the top of the shell 11 for loading salting-out raw materials, and two discharge branch pipes 28 are fixedly connected to the bottom of the silo 27, and the ends of the discharge branch pipes 28 extend to the upper side of the corresponding side discharge chamber 12, so as to facilitate the salting-out raw materials to enter the discharge chamber 12.

[0037] Specifically, the salting-out material required for preparation, such as ammonium sulfate solid particles, is poured into the silo 27, and the salting-out material will enter the two unloading chambers 12 along the unloading branch pipe 28. The worm 25 is driven to rotate by rotating the knob 26, and the rotating worm 25 is meshed with the worm wheel 21 to drive the retaining ring 19 on the outer diameter of the two unloading chambers 12 to rotate. The rotating retaining ring 19 will drive the inner internal thread 20 to mesh with the external thread 18 on the outer diameter of the movable seat 13. The movable seat 13 is driven to rise and fall by utilizing the limiting effect of the guide rod 16 and the guide groove 17. The lifting and lowering of the movable seat 13 can change the size of the space on the upper side of the unloading chamber 12, thereby changing the amount of the salting-out material entering the unloading chamber 12 each time, so as to facilitate the precise control of the unloading amount of the salting-out material.

[0038] Furthermore, a detector 33 is fixedly installed on the other side of the inner bottom of the salting-out chamber 1. It is a main instrument for detecting the components of the mixed liquid and belongs to the prior art, which will not be elaborated on here. A detection tube 34 is fixedly installed on the top of the detector 33, and a leakage tube 35 is fixedly connected to one side of the bottom of the mixed aluminum cylinder 2. A pressure ring 37 is connected to the outer position of the leakage tube 35 on one side of the bottom of the mixed aluminum cylinder 2 through a reset spring 36. A push rod 38 is fixedly installed in the middle of the pressure ring 37, and the top of the push rod 38 extends to the top of the leakage tube 35 and is fixedly installed with a sealing plug 39 for sealing the leakage tube 35.

[0039] Specifically, when the mixed aluminum cylinder 2 rotates, it will also drive the pressure ring 37 at the bottom to make a circular motion. When the bottom of the moving pressure ring 37 contacts the top of the detection tube 34, it will be pushed upward a short distance, thereby driving the push rod 38 and the sealing plug 39 to move upward, opening the top opening of the leakage tube 35, allowing the mixed liquid to enter the detection tube 34 along the leakage tube 35 and finally enter the detector 33. The detector 33 is used to perform parameter detection on the mixed liquid. As the mixed aluminum cylinder 2 continues to rotate, the pressure ring 37 and the detection tube 34 are separated. Under the action of the reset spring 36, the pressure ring 37 is reset, driving the push rod 38 and the sealing plug 39 to reset, preventing the mixed liquid from leaking out. This cycle is repeated, and the detector 33 can perform real-time parameter detection on the mixed liquid.

[0040] Furthermore, a door 40 is provided at the top front side of the salting-out chamber 1 , an observation window 41 is fixedly connected to the upper front side of the salting-out chamber 1 , and a controller 42 is fixedly installed at the lower front side of the salting-out chamber 1 .

[0041] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A preparation device for glucose oxidase, comprising a salting-out chamber (1), characterized in that: A mixed aluminum cylinder (2) is movably mounted on the upper side of the interior of the salting-out chamber (1) via a bearing seat, a central axis rod (5) is movably mounted in the middle of the interior of the salting-out chamber (1), stirring rods (9) are fixedly connected to both sides of the middle of the central axis rod (5), magnetic rods (10) are fixedly mounted on both sides of the interior of the salting-out chamber (1), a shell (11) is fixedly connected to the middle of the top of the salting-out chamber (1), a feed chamber (12) is fixedly connected to both sides of the interior of the shell (11), and the bottom ends of the feed chamber (12) are connected to the interior of the mixed aluminum cylinder (2), a movable seat (13) is movably mounted in the middle of the interior of the feed chamber (12), a feed channel (14) is provided in the middle of the movable seat (13), and the top of the feed chamber (12) is fixedly connected to the bottom of the shell (11). A stopper (22) is movably mounted, the bottom end of each of the stoppers (22) is fixedly connected to a long rod (23), and the end of each of the long rods (23) penetrates the interior of the material discharge channel (14) and extends into the interior of the mixed aluminum cylinder (2), a blocking block (24) is fixedly connected to the position of the outer diameter of the long rod (23) near the bottom of the material discharge channel (14), a cylinder body (29) is fixedly mounted on the upper outer diameter of the central axis rod (5), an inclined oblique bearing seat (30) is movably arranged on the outer diameter of the cylinder body (29), both ends of the oblique bearing seat (30) are fixedly connected to a swing rod (31), the end of each of the swing rods (31) is movably mounted to a connecting rod (32), and the end of each of the connecting rods (32) is movably mounted on the bottom of the corresponding side of the long rod (23); A three-phase asynchronous motor (3) is fixedly mounted on one side of the inner bottom of the salt precipitation chamber (1); a driving bevel gear (4) is fixedly mounted on the driving end of the three-phase asynchronous motor (3); a first driven bevel gear (6) is fixedly mounted on the lower outer diameter of the middle shaft (5); a connecting ring (7) is fixedly connected to the bottom end of the mixing aluminum cylinder (2); a second driven bevel gear (8) is fixedly connected to the bottom end of the connecting ring (7); one side of each of the first driven bevel gear (6) and the second driven bevel gear (8) is meshedly connected to the outer side end of the driving bevel gear (4); A detector (33) is fixedly mounted on the other side of the inner bottom of the salt precipitation chamber (1), a detection tube (34) is fixedly mounted on the top of the detector (33), a liquid leakage tube (35) is fixedly connected to one side of the bottom of the mixed aluminum cylinder (2), a pressure ring (37) is connected to one side of the bottom of the mixed aluminum cylinder (2) near the outer side of the liquid leakage tube (35) via a return spring (36), a push rod (38) is fixedly mounted in the middle of the pressure ring (37), and the top of the push rod (38) extends to the top of the liquid leakage tube (35) and is fixedly mounted with a sealing plug (39).

2. The preparation device of glucose oxidase according to claim 1, characterized in that: A fixed seat (15) is fixedly installed on the inner bottom of the unloading chamber (12), and guide rods (16) are fixedly connected to both sides of the interior of the fixed seat (15). Guide grooves (17) are opened on both sides of the interior of the movable seat (13), and the ends of the guide rods (16) are movably arranged inside the guide grooves (17) on the corresponding sides.

3. The preparation device of glucose oxidase according to claim 1, characterized in that: The movable seat (13) is provided with an external thread (18) on the middle outer diameter, and a clamping ring (19) is movably mounted on the lower outer diameter of the material discharge chamber (12). The inner end of the clamping ring (19) is provided with an internal thread (20), and the inner end of the internal thread (20) is threadably connected to the outer end of the clamping ring (19).

4. The preparation device of glucose oxidase according to claim 3, characterized in that: The outer ends of the snap rings (19) are fixedly connected to a worm wheel (21), a worm (25) is movably mounted inside the housing (11), and both sides of the worm (25) are meshedly connected to the inner side of the worm wheel (21), and one end of the worm (25) extends to the outside of the housing (11) and is fixedly mounted with a knob (26).

5. The preparation device of glucose oxidase according to claim 1, characterized in that: A material bin (27) is fixedly connected to the top of the shell (11), and two material discharge branch pipes (28) are fixedly connected to the bottom of the material bin (27), and the ends of the material discharge branch pipes (28) extend to the upper inner side of the corresponding material discharge chamber (12).

6. The preparation device of glucose oxidase according to claim 1, characterized in that: A door (40) is provided on the top front side of the salting-out chamber (1), an observation window (41) is fixedly connected to the upper front side of the salting-out chamber (1), and a controller (42) is fixedly installed on the lower front side of the salting-out chamber (1).

Images