Formula and preparation method of composite black fungus blood fat reducing product

Abstract

The invention provides a composite black fungus blood fat reducing product formula and a preparation method thereof, and belongs to the technical field of biology. On the first aspect, the invention provides a formula of a composite black fungus product for reducing blood fat, and the formula of the composite black fungus product for reducing blood fat comprises the following components: black fungus enzymolysis macromolecules, an active activator, a mulberry leaf extract and trehalose; secondly, the invention provides a preparation method of the composite black fungus blood fat reducing product. Thirdly, the invention provides a preparation method of the black fungus enzymolysis macromolecules. In the fourth aspect, the invention provides a preparation method of raspberry fermentation broth freeze-dried powder.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a formula of a composite black fungus blood lipid lowering product and a preparation method thereof. Background Art

[0002] As people's living standards continue to improve, the large intake of high-energy and high-fat foods, the increase in alcohol consumption and the reduction in exercise have led to a clear upward trend in the incidence of hyperlipidemia, which is an important factor in inducing coronary atherosclerotic heart disease (CHD), arteriosclerosis, fatty liver, diabetes, obesity, etc. According to preliminary estimates, the prevalence of hyperlipidemia in my country is over 18%, which has become the main culprit threatening the lives of middle-aged and elderly people. Hyperlipidemia is one of the important risk factors for cardiovascular and cerebrovascular diseases.

[0003] Hyperlipidemia is one of the common and frequently occurring modern intractable diseases, which is mainly manifested by excessively high levels of serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL-C) and (or) low levels of high-density lipoprotein (HDL-C). Long-term high-fat diet can easily reduce the activity of lipid metabolism enzymes such as hepatic lipase (HL) and lipoprotein esterase (LPL), which can easily lead to lipid metabolism disorders. Modern medicine believes that a high-fat diet is a clear risk factor for hyperlipidemia, and hyperlipidemia is an important and clear risk factor for hypertension, coronary heart disease, atherosclerosis (AS), diabetes and other diseases. Therefore, the prevention and treatment of this disease has received increasing attention from the medical community.

[0004] With the improvement of health awareness and the intensification of the aging trend, the market size of blood lipid-lowering products continues to expand. In recent years, due to factors such as improved living standards and bad eating habits, the number of patients with hyperlipidemia has continued to rise, and consumers' demand for blood lipid-lowering products has also increased. It is expected that in the next few years, as consumers pay more attention to health management, the blood lipid-lowering product market will continue to grow.

[0005] At present, there are many types of lipid-lowering products on the market, mainly including two categories: pharmaceuticals and non-pharmaceuticals. Pharmaceuticals are mainly prescription drugs, such as statins and fibrates, which lower blood lipid levels through specific drug mechanisms; non-pharmaceuticals include health products, nutritional supplements, health foods, etc., which are mostly based on plant extracts, dietary fiber, deep-sea fish oil, etc., and are supplemented daily to assist in regulating blood lipids.

[0006] Although the lipid-lowering product market is developing rapidly, it still faces some problems and challenges. First, there is a serious problem of product homogeneity in the market. The functions of lipid-lowering products of different brands are similar, making it difficult to form differentiated competition. Second, some consumers have misunderstandings about lipid-lowering products, thinking that as long as they take the products, their blood lipid status can be quickly improved, ignoring the importance of a reasonable diet and lifestyle. In addition, the side effects and safety issues of lipid-lowering products have also attracted much attention, such as some drugs may have adverse effects on the liver.

[0007] Black fungus, also known as black vegetable, fungus, and cloud fungus, belongs to the Auriculariaceae family, Auricularia genus. It is a precious colloid fungus that can be used as both medicine and food in my country. It is also a recognized health food in the world.

[0008] Since ancient times, black fungus has been a famous edible and medicinal fungus in my country. It is the best edible product and is known as the king of precious edible fungi. Black fungus has the functions of lowering blood lipids, anti-thrombosis, anti-aging, and anti-tumor. Whether it is eaten directly or used as a food formula ingredient, it is an ideal health food resource.

[0009] According to traditional Chinese medicine, black fungus is sweet and mild in nature, and has the effects of clearing the lungs and moistening the intestines, nourishing yin and blood, promoting blood circulation and removing blood stasis, improving eyesight and nourishing the stomach. It can be used to treat symptoms such as metrorrhagia, hemorrhoids, bloody diarrhea, anemia and constipation. At the same time, the fermentation elements and alkaloids it contains can promote the secretion of glands in the digestive tract and urinary tract, and cooperate with the secretions to catalyze stones, and have a significant dissolving effect on gallstones and kidney stones. Modern medicinal research on black fungus began in the 1980s, and it was first discovered that it has a significant inhibitory effect on platelet aggregation, which attracted the attention of the world. Wu Wutong and others extracted and separated the important active ingredient black fungus polysaccharide from black fungus. In the following 20 years, a large number of pharmacological studies on black fungus polysaccharides have been carried out, revealing the wide physiological activity and extremely high medicinal and health care functions of black fungus.

[0010] Black fungus polysaccharide has the effect of inhibiting platelet aggregation, and its mechanism is mainly to inhibit the activity of thrombin. Domestic studies have shown that in vitro tests with 0.1mL of 30μmol / L polysaccharide solution mixed with 0.9mL of rabbit blood can extend the coagulation time by 2 times. Mice were divided into groups for intravenous injection, intraperitoneal injection and oral gavage of 50mg / kg, and the coagulation time was extended by 2.1, 1.3 and 1.3 times compared with the control group, respectively. Studies have reported that the lipid-lowering effect of black fungus polysaccharide was administered to hyperlipidemia mice at a daily dose of 300mg / kg for 12 weeks. It was determined that the total cholesterol content and atherosclerosis index of mice serum can be significantly reduced, and the antioxidant capacity of serum and liver can be improved. Cai Xiaoling et al. injected black fungus polysaccharide into hyperlipidemia mice every day, and the cholesterol content in the mouse serum was significantly reduced after one week. Zhou Guohua et al. conducted experiments on high-fat model mice using black fungus polysaccharides at different concentrations. The results showed that the serum triglycerides, total cholesterol and low-density lipoprotein of the mice in the black fungus polysaccharide group were lower than those in the control group to varying degrees, while the high-density lipoprotein was significantly higher than that in the control group, indicating that black fungus polysaccharides have a lipid-lowering effect.

[0011] Shen Jianhe et al. found in their test results on rabbits that black fungus polysaccharide can significantly prolong the formation time of specific thrombus and fibrin thrombus, shorten the length of thrombus, and reduce the wet weight and dry weight of thrombus. In addition, black fungus polysaccharide also has multiple effects such as anti-ulcer, anti-hepatitis, anti-infection, anti-mutation, and promoting nucleic acid and protein biosynthesis.

[0012] In view of this, in order to overcome the problems existing in blood lipid lowering products, the present invention provides a composite black fungus blood lipid lowering product formula and a preparation method thereof through technological innovation. Summary of the invention

[0013] The purpose of the present invention is to provide a formula of a composite black fungus blood lipid lowering product and a preparation method thereof.

[0014] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0015] In a first aspect, the present invention provides a composite black fungus lipid-lowering product formula, which comprises black fungus enzymatic hydrolysis macromolecules, an active activator, mulberry leaf extract, and trehalose.

[0016] Furthermore, in a preferred embodiment of the present invention, by weight, the black fungus enzymatic hydrolyzed macromolecules are 280-320 parts, the activity activator is 18-22 parts, the mulberry leaf extract is 50-60 parts, and the trehalose is 56-64 parts.

[0017] Furthermore, in a preferred embodiment of the present invention, by weight, the black fungus enzymatic hydrolyzed macromolecules are 300 parts, the activity activator is 20 parts, the mulberry leaf extract is 55 parts, and the trehalose is 60 parts.

[0018] Furthermore, in a preferred embodiment of the present invention, the active activator is composed of phytosaccharide and freeze-dried powder of raspberry fermentation broth.

[0019] Furthermore, in a preferred embodiment of the present invention, the weight ratio of the euphorbia polysaccharide to the freeze-dried powder of raspberry fermentation broth is 20:25-35.

[0020] In a second aspect, the present invention provides a method for preparing a composite black fungus hypolipidemic product, the specific process being:

[0021] The specific process of the preparation method is:

[0022] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder at a weight ratio of 20:25-35;

[0023] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0024] Furthermore, in a preferred embodiment of the present invention, the euphorbia polysaccharide and the freeze-dried powder of raspberry fermentation broth are mixed in a weight ratio of 20:30.

[0025] In a third aspect, the present invention also provides a method for preparing black fungus enzymatic macromolecules, the specific process is: taking black fungus powder, adding acetone, the liquid-to-solid ratio is 1:25-35, reflux for 2-4 hours to defat, filter, and dry the defatted black fungus powder at 60°C to constant weight; then extract with hot water, the extraction temperature is 98°C, the extraction time is 4-6 hours, the liquid-to-solid ratio is 1:40-60, centrifuge, extract twice, combine the supernatant and treat separately; add water to the black fungus residue after centrifugation, the liquid-to-solid ratio is 1:25-35, add complex enzyme consisting of hemicellulase 800-1200U / L, pectinase 400-600U / L, and papain 200-400U / L, perform enzymolysis at 36-40°C for 5-7 hours, then inactivate at 100°C for 5 minutes, collect the supernatant by centrifugation, pass through A845 weak acid acrylic resin column, collect the supernatant, then separate with 1000D membrane, take the retained portion, freeze-dry, and obtain black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

[0026] Further, in a preferred embodiment of the present invention, the specific process of the preparation method is: take black fungus powder, add acetone, the liquid-to-solid ratio is 1:30, reflux for 3h to defatting, filter, and dry the defatted black fungus powder at 60°C to constant weight; then extract with hot water, the extraction temperature is 98°C, the extraction time is 5h, the liquid-to-solid ratio is 1:50, centrifuge, extract twice, combine the supernatant and treat it separately; add water to the black fungus residue after centrifugation, the liquid-to-solid ratio is 1:30, add a composite enzyme, the composite enzyme consists of 1000U / L hemicellulase, 500U / L pectinase, and 300U / L papain, enzymolysis at 38°C for 6 hours, then inactivate at 100°C for 5 minutes, centrifuge to collect the supernatant, pass through an A845 weak acid acrylic resin column, collect the supernatant, then separate with a 1000D membrane, take the retained portion, freeze-dry, and obtain black fungus enzymatic macromolecular freeze-dried powder.

[0027] In a fourth aspect, the present invention further provides a method for preparing freeze-dried powder of raspberry fermented liquid, the specific process being:

[0028] Raspberries were crushed according to a conventional method, and then an equal weight of white eyed bean superfine powder and 2 times the weight of water were added, the pH value was adjusted to 7.0-7.2, sterilized at 121°C for 20 minutes, inoculated with Lactobacillus bulgaricus at 42-48°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, and the supernatant was collected by centrifugation, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, the supernatant was collected, appropriately concentrated and then freeze-dried to obtain freeze-dried powder.

[0029] Furthermore, in a preferred embodiment of the present invention, the specific process of the preparation method is:

[0030] Raspberries were crushed according to a conventional method, and then an equal weight of white eyebrow bean superfine powder and 2 times the weight of water were added, the pH value was adjusted to 7.1, sterilized at 121°C for 20 minutes, inoculated with Lactobacillus bulgaricus at 45°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, the supernatant was collected by centrifugation, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, the supernatant was collected, appropriately concentrated and then freeze-dried to obtain freeze-dried powder.

[0031] Compared with the prior art, the beneficial effects of the present invention include:

[0032] Compared with the prior art, the invention has significant improvements in relieving diarrhea, especially in lowering blood lipids. DETAILED DESCRIPTION

[0033] The embodiments of the present invention will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. If specific conditions are not specified in the examples, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0034] The features and properties of the present invention are further described in detail below in conjunction with the embodiments:

[0035] Example 1

[0036] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 280 parts of black fungus enzymatic hydrolyzed macromolecules, 22 parts of active activators, 50 parts of mulberry leaf extracts, and 64 parts of trehalose.

[0037] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0038] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:25;

[0039] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0040] The black fungus enzymatic hydrolysis macromolecule is obtained by enzymatic hydrolysis of black fungus residue. The preparation method of the black fungus enzymatic hydrolysis macromolecule has the following specific process:

[0041] 50 kg of black fungus powder was taken, acetone was added, the liquid-to-solid ratio was 1:35, refluxed for 2 hours for degreasing, filtered, and the defatted black fungus powder was dried at 60° C. to constant weight; hot water was used for extraction, the extraction temperature was 98° C., the extraction time was 6 hours, the liquid-to-solid ratio was 1:40, centrifuged, extracted twice, and the supernatant was combined and processed separately; water was added to the black fungus residue after centrifugation, the liquid-to-solid ratio was 1:35, and a composite enzyme was added, the composite enzyme consisted of 800 U / L hemicellulase, 600 U / L pectinase, and 200 U / L papain, enzymolysis was performed at 40° C. for 5 hours, and then inactivated at 100° C. for 5 minutes, the supernatant was collected by centrifugation, passed through an A845 weak acid acrylic resin column, the supernatant was collected, and then separated by a 1000D membrane, the intercepted portion was taken, and freeze-dried to obtain 983.5 g of black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

[0042] The preparation method of raspberry fermented liquid freeze-dried powder, the specific process is:

[0043] 1 kg of raspberries were crushed according to the conventional method, and an equal weight of white eyebrow bean superfine powder and 2 times the weight of water were added respectively, the pH value was adjusted to 7.2, sterilized at 121°C for 20 min, inoculated with Lactobacillus bulgaricus at 42°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, centrifuged to collect the supernatant, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, collected the supernatant, appropriately concentrated and then freeze-dried to obtain 30.4 g of freeze-dried powder.

[0044] Example 2

[0045] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 320 parts of black fungus enzymatic hydrolyzed macromolecules, 18 parts of active activators, 60 parts of mulberry leaf extracts, and 56 parts of trehalose.

[0046] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0047] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:35;

[0048] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0049] The black fungus enzymatic hydrolysis macromolecule is obtained by enzymatic hydrolysis of black fungus residue. The preparation method of the black fungus enzymatic hydrolysis macromolecule has the following specific process:

[0050] Take 50 kg of black fungus powder, add acetone, the liquid-to-solid ratio is 1:25, reflux for 4 hours to defat, filter, and dry the defatted black fungus powder at 60°C to constant weight; then use hot water for extraction, the extraction temperature is 98°C, the extraction time is 4 hours, the liquid-to-solid ratio is 1:60, centrifuge, extract twice, combine the supernatants and treat them separately; add water to the black fungus residue after centrifugation, the liquid-to-solid ratio is 1:25, add complex enzyme, the complex enzyme consists of 1200 U / L hemicellulase, 400 U / L pectinase, and 400 U / L papain, enzymolyze at 36°C for 7 hours, then inactivate at 100°C for 5 minutes, centrifuge to collect the supernatant, pass through an A845 weak acid acrylic resin column, collect the supernatant, then separate with a 1000D membrane, take the intercepted part, freeze-dry, and obtain 957.3 g of black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

[0051] The preparation method of raspberry fermented liquid freeze-dried powder, the specific process is:

[0052] 1 kg of raspberries were crushed according to the conventional method, and an equal weight of white eyebrow bean superfine powder and 2 times the weight of water were added respectively, the pH value was adjusted to 7.0, sterilized at 121°C for 20 min, inoculated with Lactobacillus bulgaricus at 48°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, centrifuged to collect the supernatant, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, collected the supernatant, appropriately concentrated and then freeze-dried to obtain 30.8 g of freeze-dried powder.

[0053] Example 3

[0054] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0055] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0056] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0057] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0058] The black fungus enzymatic hydrolysis macromolecule is obtained by enzymatic hydrolysis of black fungus residue. The preparation method of the black fungus enzymatic hydrolysis macromolecule has the following specific process:

[0059] Take 50 kg of black fungus powder, add acetone, the liquid-to-solid ratio is 1:30, reflux for 3 hours to defat, filter, and dry the defatted black fungus powder at 60°C to constant weight; then use hot water for extraction, the extraction temperature is 98°C, the extraction time is 5 hours, the liquid-to-solid ratio is 1:50, centrifuge, extract twice, combine the supernatants and treat them separately; add water to the black fungus residue after centrifugation, the liquid-to-solid ratio is 1:30, add complex enzyme, the complex enzyme consists of 1000 U / L hemicellulase, 500 U / L pectinase, and 300 U / L papain, enzymolyze at 38°C for 6 hours, then inactivate at 100°C for 5 minutes, centrifuge to collect the supernatant, pass through an A845 weak acid acrylic resin column, collect the supernatant, then separate with a 1000D membrane, take the intercepted part, freeze-dry, and obtain 1028.9 g of black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

[0060] The preparation method of raspberry fermented liquid freeze-dried powder, the specific process is:

[0061] 1 kg of raspberries were crushed according to the conventional method, and an equal weight of white eyebrow bean superfine powder and 2 times the weight of water were added respectively, the pH value was adjusted to 7.1, sterilized at 121°C for 20 min, inoculated with Lactobacillus bulgaricus at 45°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, centrifuged to collect the supernatant, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, collected the supernatant, appropriately concentrated and then freeze-dried to obtain 31.5 g of freeze-dried powder.

[0062] Comparative Example 1

[0063] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus superfine powder, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0064] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0065] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0066] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0067] The black fungus ultrafine powder is prepared by the following method: taking black fungus, removing surface impurities and hard root parts, washing and drying, and then performing preliminary crushing with a cyclone crusher, passing through a 100-mesh sieve to obtain coarse powder, and then performing airflow ultrafine crushing on the coarse powder with an airflow crusher, and drying in a vacuum drying oven to constant weight to obtain black fungus ultrafine powder, wherein the particle size is 10 to 50 μm and the average particle size is 25 μm.

[0068] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0069] Comparative Example 2

[0070] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus water extract, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0071] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0072] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0073] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0074] The black fungus water extract is prepared by the following method: taking 1 kg of black fungus powder, adding acetone, the liquid-to-solid ratio is 1:30, reflux for 3 hours to defatted, filtering, and drying the defatted black fungus powder at 60°C to constant weight; then extracting with hot water, the extraction temperature is 98°C, the extraction time is 5 hours, the liquid-to-solid ratio is 1:50, centrifuging, extracting twice, combining the supernatant, freeze-drying, and obtaining 83.6 g of black fungus water extract dry powder.

[0075] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0076] Comparative Example 3

[0077] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus polysaccharide, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0078] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0079] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0080] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0081] Black fungus polysaccharide is prepared by the following method: taking 1 kg of black fungus powder, adding acetone, the liquid-to-solid ratio is 1:30, reflux for 3 hours to defatted, filtering, and drying the defatted black fungus powder at 60°C to constant weight; then extracting with hot water, the extraction temperature is 98°C, the extraction time is 5 hours, the liquid-to-solid ratio is 1:50, centrifuging, extracting twice, combining the supernatants, passing through an A845 weak acid acrylic resin column, collecting the supernatants, and then separating with a 1000D membrane, taking the retained portion, freeze-drying, and obtaining 24.5 g of black fungus polysaccharide dry powder.

[0082] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0083] Comparative Example 4

[0084] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0085] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0086] (1) Preparation of active activator: Spirulina polysaccharide and raspberry fermentation broth freeze-dried powder are mixed at a weight ratio of 20:30;

[0087] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0088] The method for preparing black fungus enzymatic macromolecules has the same specific process as Example 3.

[0089] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0090] Comparative Example 5

[0091] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0092] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0093] (1) Preparation of active activator: mix phytosaccharide and strawberry enzymatic hydrolysate freeze-dried powder in a weight ratio of 20:30;

[0094] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0095] The method for preparing black fungus enzymatic macromolecules has the same specific process as Example 3.

[0096] The method for preparing freeze-dried powder of strawberry enzymatic hydrolysate is the same as the method for preparing freeze-dried powder of raspberry fermentation liquid in Example 3.

[0097] Comparative Example 6

[0098] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0099] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0100] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry freeze-dried powder in a weight ratio of 20:30;

[0101] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0102] The method for preparing black fungus enzymatic macromolecules has the same specific process as Example 3.

[0103] The preparation method of raspberry freeze-dried powder comprises the following specific processes: taking 1 kg of raspberries, crushing them according to a conventional method, and freeze-drying them to obtain freeze-dried powder.

[0104] Comparative Example 7

[0105] The formula of the composite lipid-lowering product includes the following components by weight: 300 grams of starch, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0106] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0107] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0108] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0109] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0110] Comparative Example 8

[0111] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0112] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0113] (1) Preparation of active activator: mix phytoplankton polysaccharide and starch at a weight ratio of 20:30;

[0114] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0115] The method for preparing black fungus enzymatic macromolecules has the same specific process as Example 3.

[0116] Comparative Example 9

[0117] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0118] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0119] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0120] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0121] The black fungus enzymatic hydrolysis macromolecule is obtained by enzymatic hydrolysis of black fungus residue. The preparation method of the black fungus enzymatic hydrolysis macromolecule has the following specific process:

[0122] 5 kg of black fungus powder was taken, acetone was added, the liquid-to-solid ratio was 1:30, refluxed for 3 hours for degreasing, filtered, and the defatted black fungus powder was dried at 60° C. to constant weight; hot water was used for extraction, the extraction temperature was 98° C., the extraction time was 5 hours, the liquid-to-solid ratio was 1:50, centrifuged, extracted twice, and the supernatant was combined and processed separately; water was added to the black fungus residue after centrifugation, the liquid-to-solid ratio was 1:30, and a composite enzyme was added, the composite enzyme consisted of 1000 U / L hemicellulase, 500 U / L pectinase, and 300 U / L ficin, enzymolysis was performed at 38° C. for 6 hours, and then inactivated at 100° C. for 5 minutes, the supernatant was collected by centrifugation, passed through an A845 weak acid acrylic resin column, the supernatant was collected, and then separated by a 1000D membrane, the intercepted portion was taken, and freeze-dried to obtain 114.5 g of black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

[0123] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0124] Comparative Example 10

[0125] The preparation method of raspberry fermented liquid freeze-dried powder, the specific process is:

[0126] 1 kg of raspberries were crushed according to the conventional method, and an equal weight of superfine soybean powder and 2 times the weight of water were added respectively, the pH value was adjusted to 7.1, sterilized at 121°C for 20 min, inoculated with Lactobacillus bulgaricus at 45°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, centrifuged to collect the supernatant, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, collected the supernatant, appropriately concentrated and then freeze-dried to obtain 36.1 g of freeze-dried powder.

[0127] The rest is the same as in Example 3.

[0128] Comparative Example 11

[0129] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0130] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0131] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0132] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0133] The black fungus enzymatic hydrolysis macromolecule is obtained by enzymatic hydrolysis of black fungus residue. The preparation method of the black fungus enzymatic hydrolysis macromolecule has the following specific process:

[0134] 5 kg of black fungus powder was taken, acetone was added, the liquid-to-solid ratio was 1:30, refluxed for 3 hours for degreasing, filtered, and the defatted black fungus powder was dried at 60° C. to constant weight; hot water was used for extraction, the extraction temperature was 98° C., the extraction time was 5 hours, the liquid-to-solid ratio was 1:50, centrifuged, extracted twice, and the supernatant was combined and processed separately; water was added to the black fungus residue after centrifugation, the liquid-to-solid ratio was 1:30, and a composite enzyme was added, the composite enzyme consisted of 1000 U / L hemicellulase, 500 U / L pectinase, and 300 U / L papain, enzymolysis was performed at 38° C. for 6 hours, and then inactivated at 100° C. for 5 minutes, the supernatant was collected by centrifugation, passed through a DOWS MSA-2 type macroporous II strong alkaline styrene anion exchange resin column, the supernatant was collected, and then separated by a 1000D membrane, the intercepted portion was taken, and freeze-dried to obtain 136.4 g of black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

[0135] The method for preparing freeze-dried powder of raspberry fermented liquid is the same as that in Example 3.

[0136] Comparative Example 12

[0137] The formula of the composite black fungus lipid-lowering product includes the following components by weight: 300 grams of black fungus enzymatic hydrolyzed macromolecules, 20 grams of active activator, 55 grams of mulberry leaf extract, and 60 grams of trehalose.

[0138] The preparation method of the composite black fungus lipid-lowering product formula adopts a dry process production, including the following steps: active activator preparation, crushing, screening, premixing, total mixing, filling with inert gas, subpackaging, packaging, the specific process is:

[0139] (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder in a weight ratio of 20:30;

[0140] (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

[0141] The preparation method of raspberry fermented liquid freeze-dried powder, the specific process is:

[0142] Take 1 kg of raspberries and crush them according to the conventional method, then add an equal weight of white eyebrow bean superfine powder and 2 times the weight of water, adjust the pH value to 7.1, sterilize at 121°C for 20 min, inoculate Lactobacillus bulgaricus at 45°C, culture in a closed manner for 3 days, and then inactivate at 100°C for 5 minutes, collect the supernatant by centrifugation, pass through an A845 weak acid acrylic resin column, collect the supernatant, appropriately concentrate and then freeze-dry to obtain 28.6 g of freeze-dried powder.

[0143] The method for preparing black fungus enzymatic macromolecules has the same specific process as Example 3.

[0144] The beneficial effects of the present invention are shown in the following table:

[0145] Experimental Example 1 Effect on Diarrhea Rate in Mice

[0146] Male mice were randomly divided into 10 groups, namely normal control group, model group, examples and comparative groups. Except for the normal control group, the rest groups were intraperitoneally injected with 0.4 mL 5×10 8 The model was successfully established when the mice showed mental depression, decreased activity, perianal dirtiness, and loose stools.

[0147] After successful modeling, medication was started, once a day, for 15 consecutive days. The dosage of each group of mice in the embodiment and the comparative example was 0.3 g sample / kg body weight, and the mice in the normal control group and the model group were gavaged with the same weight of starch. On the 16th day, the diarrhea rate of the experimental mice was measured.

[0148] Diarrhea rate = number of animals with diarrhea / total number of animals in the group × 100%.

[0149] Table 1 Effect on diarrhea rate of mice

[0150] Diarrhea rate (%) Normal control group 0 Model Group 90 Example 1 20 Example 2 10 Example 3 10 Comparative Example 1 60 Comparative Example 2 70 Comparative Example 3 50 Comparative Example 4 60 Comparative Example 5 80 Comparative Example 6 70 Comparative Example 7 50 Comparative Example 8 60 Comparative Example 9 80 Comparative Example 10 40 Comparative Example 11 70 Comparative Example 12 60

[0151] Experimental Example 2 Effects on blood lipids in hyperlipidemia mice

[0152] Male mice were randomly divided into groups, 10 in each group, namely, a normal control group, a model group, each group of the embodiment, and each group of the comparative example. Except for the normal control group, which was fed with a basic feed, the mice in the other groups were fed with a high-fat feed, which was freely ingested without quantity. After 30 days, the mice in each group of the embodiment and the comparative example were gavaged with 0.15g sample / kg body weight, and the mice in the normal control group and the model group were gavaged with an equal weight of starch. Except for the normal control group, which was fed with a basic feed, the mice in the other groups were fed with a high-fat feed. After the last administration on the 60th day, the mice were fasted, whole blood was collected by the eyeball blood method, and the blood was left to stand for 30 minutes. The serum was separated by centrifugation, and the TC, TG, HDL-C, and LDL-C contents in the serum were determined.

[0153] High-fat feed formula: take 1% cholesterol, 5% egg yolk powder, 10% lard, 5% fructose, 0.2% sodium cholate, and 78.8% basic feed, mix them thoroughly, press them into round strips, dry them, pack them into bags, and sterilize them by irradiation.

[0154] Table 2 Effects on blood lipids in hyperlipidemia mice

[0155]

[0156]

[0157] In summary, compared with the comparative examples, the relevant technical effects of the embodiments of the present invention are significantly improved.

[0158] The links not described in detail in the present invention are all common knowledge that can be selected by ordinary technicians in the field. Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to ordinary technicians in the field that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A composite black fungus lipid-lowering product formula, characterized in that: The formula of the composite black fungus blood lipid lowering product comprises the following components: black fungus enzymatic hydrolysis macromolecules, an active activator, a mulberry leaf extract, and trehalose.

2. The composite black fungus blood lipid lowering product formula according to claim 1, characterized in that: In terms of weight, the black fungus enzymatic hydrolysis macromolecules are 280 to 320 parts, the activity activator is 18 to 22 parts, the mulberry leaf extract is 50 to 60 parts, and the trehalose is 56 to 64 parts.

3. The composite black fungus hypolipidemic product formula according to claim 1, characterized in that: In terms of weight, the black fungus enzymatic hydrolysis macromolecules are 300 parts, the activity activator is 20 parts, the mulberry leaf extract is 55 parts, and the trehalose is 60 parts.

4. The composite black fungus hypolipidemic product formula according to claim 1, characterized in that: The active activator consists of phytoplankton polysaccharide and freeze-dried powder of raspberry fermentation liquid.

5. The composite black fungus blood lipid lowering product formula according to claim 4, characterized in that: In terms of weight, the weight ratio of the euphorbia polysaccharide to the freeze-dried powder of raspberry fermentation liquid is 20:25-35.

6. A method for preparing the composite black fungus hypolipidemic product according to claim 1, characterized in that: The specific process of the preparation method is: (1) Preparation of active activator: mix phytoplankton polysaccharide and raspberry fermentation broth freeze-dried powder at a weight ratio of 20:25-35; (2) Weigh the above raw materials by weight, crush them into 80 mesh, sieve them through a 50 mesh sieve, pre-mix them by equal incremental method for 25 minutes, mix them in a three-dimensional motion mixer for 30 minutes, fill them with nitrogen to control the residual oxygen content to less than 3%, and package them to obtain a composite black fungus hypolipidemic product.

7. A method for preparing black fungus enzymatic macromolecules according to claim 1, characterized in that: The specific process of the preparation method is as follows: taking black fungus powder, adding acetone, the liquid-to-solid ratio is 1:25-35, refluxing for 2-4 hours to defat, filtering, and drying the defatted black fungus powder at 60° C. to constant weight; then extracting with hot water, the extraction temperature is 98° C., the extraction time is 4-6 hours, the liquid-to-solid ratio is 1:40-60, centrifuging, extracting twice, combining the supernatant and treating it separately; adding water to the black fungus residue after centrifugation, the liquid-to-solid ratio is 1:25-35, adding The composite enzyme consists of 800-1200 U / L of hemicellulase, 400-600 U / L of pectinase and 200-400 U / L of papain, and is enzymolyzed at 36-40°C for 5-7 hours, then inactivated at 100°C for 5 minutes, and the supernatant is collected by centrifugation, passed through an A845 weak acid acrylic resin column, and then separated by a 1000D membrane, and the retained portion is taken and freeze-dried to obtain black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

8. The method for preparing black fungus enzymatic macromolecules according to claim 7, characterized in that: The specific process of the preparation method is as follows: taking black fungus powder, adding acetone, the liquid-to-solid ratio is 1:30, refluxing for 3 hours to defat, filtering, and drying the defatted black fungus powder at 60°C to constant weight; then extracting with hot water, the extraction temperature is 98°C, the extraction time is 5 hours, the liquid-to-solid ratio is 1:50, centrifuging, extracting twice, combining the supernatant and treating separately; adding water to the black fungus residue after centrifugation, the liquid-to-solid ratio is 1:30, adding a composite enzyme, the composite enzyme consists of 1000U / L hemicellulase, 500U / L pectinase, and 300U / L papain, enzymolysis at 38°C for 6 hours, and then inactivating at 100°C for 5 minutes, centrifuging and collecting the supernatant, passing through an A845 weak acid acrylic resin column, collecting the supernatant, and then separating with a 1000D membrane, taking the intercepted part, freeze-drying, and obtaining black fungus enzymatic hydrolysis macromolecular freeze-dried powder.

9. A method for preparing freeze-dried powder of raspberry fermented liquid according to claim 4, characterized in that: The specific process of the preparation method is: Raspberries were crushed according to a conventional method, and then an equal weight of white eyebrow bean superfine powder and 2 times the weight of water were added, the pH value was adjusted to 7.0-7.2, sterilized at 121°C for 20 minutes, inoculated with Lactobacillus bulgaricus at 42-48°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, and the supernatant was collected by centrifugation, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, the supernatant was collected, appropriately concentrated and then freeze-dried to obtain freeze-dried powder.

10. The method for preparing freeze-dried powder of raspberry fermented liquid according to claim 9, characterized in that: The specific process of the preparation method is: Raspberries were crushed according to a conventional method, and then an equal weight of white eyebrow bean superfine powder and 2 times the weight of water were added, the pH value was adjusted to 7.1, sterilized at 121°C for 20 minutes, inoculated with Lactobacillus bulgaricus at 45°C, cultured in a closed manner for 3 days, and then inactivated at 100°C for 5 minutes, the supernatant was collected by centrifugation, passed through a DOWS MSA-2 macroporous II strong alkaline styrene anion exchange resin column, the supernatant was collected, appropriately concentrated and then freeze-dried to obtain freeze-dried powder.

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