Bio-enzyme laundry detergent and preparation method thereof

Abstract

The invention provides a bio-enzyme laundry detergent and a preparation method thereof, and belongs to the technical field of laundry detergents. The bio-enzyme immobilized microspheres are prepared from the following raw materials in parts by weight: 7-12 parts of bio-enzyme immobilized microspheres, 10-15 parts of a surfactant, 100-120 parts of deionized water, 0.1-0.2 part of a preservative and 0.15-0.3 part of essence. According to the preparation method, glutaric acid and sodium alginate are subjected to esterification reaction cross-linking and then are further solidified under the action of calcium chloride to form the immobilized microspheres, and hydrogen bonds are formed through hydroxyl and the composite biological enzyme for complexing immobilization, so that the biological enzyme immobilized microspheres are prepared, the influence of alkaline conditions on the activity of the biological enzyme can be effectively avoided, and the biological enzyme activity is improved. The stability of the prepared laundry detergent can be improved, the shelf life is prolonged, and the laundry detergent has a relatively good washing effect and a wide application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of laundry detergents, and in particular to a bio-enzyme laundry detergent and a preparation method thereof. Background Art

[0002] Laundry detergent is a liquid detergent. Compared with traditional washing powder, laundry detergent has the advantages of being neutral, mild, gentle, and non-irritating to clothes and skin. Replacing washing powder and soap with laundry detergent can reduce environmental pollution. In recent years, the demand for laundry detergent has increased significantly. However, it is difficult to add biological enzymes to laundry detergent, which makes the cleaning ability of laundry detergent weaker and limits its application. The biological enzymes added to laundry detergent include protease, lipase, cellulase, lysozyme, amylase, etc. Since the biological enzyme itself is also a protein, it will self-digest in the water phase, so the water content in the formula needs to be as small as possible. The laundry detergent with enzymes added on the market is concentrated, and the water content needs to be less than 50%; at the same time, in order to ensure the activity of the biological enzymes in the laundry detergent, enzyme stabilizers need to be added, which will increase the cost of the laundry detergent; irregular transportation and storage conditions will also affect the stability of the enzyme; the pH value of the laundry detergent is alkaline, and the biological enzyme is very easy to deteriorate in an alkaline environment; some surfactants have a great influence on the stability of the biological enzyme, such as the fatty alcohol polyoxyethylene ether sodium sulfate (AES) commonly used in laundry detergent. The laundry detergent with AES added will significantly reduce the activity of the biological enzyme. In addition, without adding any protection, the retention period of the biological enzyme in the laundry detergent generally does not exceed three months, which is relatively short. Summary of the invention

[0003] The purpose of the present invention is to provide a bio-enzyme laundry detergent and a preparation method thereof, which can effectively avoid the influence of alkaline conditions on the activity of the bio-enzyme, improve the stability of the prepared laundry detergent, extend the shelf life, and have a good washing effect, thus having broad application prospects.

[0004] The technical solution of the present invention is achieved in this way:

[0005] The invention provides a bioenzyme laundry detergent, which is prepared from the following raw materials in parts by weight: 7-12 parts of bioenzyme immobilized microspheres, 10-15 parts of surfactants, 100-120 parts of deionized water, 0.1-0.2 parts of preservatives, and 0.15-0.3 parts of essences.

[0006] As a further improvement of the present invention, the surfactant includes a hydrophilic emulsifier and a lipophilic emulsifier in a mass ratio of 12-15:7-10.

[0007] As a further improvement of the present invention, the hydrophilic emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85.

[0008] As a further improvement of the present invention, the lipophilic emulsifier is selected from at least one of Span-20, Span-40, Span-60, Span-80 and Span-85.

[0009] As a further improvement of the present invention, the preparation method of the bio-enzyme immobilized microspheres is as follows:

[0010] S1. Add sodium alginate to dimethylformamide, add glutaric acid and a catalyst, heat to reflux and stir to react, add calcium chloride solution dropwise, centrifuge, wash, and dry to obtain immobilized microspheres;

[0011] S2. Add the composite bio-enzyme into water, add the immobilized microspheres, and freeze-dry to obtain the bio-enzyme immobilized microspheres.

[0012] As a further improvement of the present invention, the mass ratio of sodium alginate, glutaric acid and catalyst in step S1 is 10-12:3-5:0.1-0.2.

[0013] As a further improvement of the present invention, the concentration of the calcium chloride solution in step S1 is 2-4wt%, the catalyst is concentrated sulfuric acid, and the stirring reaction time is 10-12h.

[0014] As a further improvement of the present invention, the composite biological enzyme in step S2 is one or both of protease and lipase.

[0015] As a further improvement of the present invention, the mass ratio of the composite biological enzyme to the immobilized microspheres in step S2 is 3-5:10.

[0016] The present invention further protects a method for preparing the above-mentioned bio-enzyme laundry detergent, comprising the following steps: mixing bio-enzyme immobilized microspheres, surfactant, deionized water, preservative and essence uniformly, discharging the materials, and preparing the bio-enzyme laundry detergent.

[0017] The present invention has the following beneficial effects:

[0018] The present invention adopts glutaric acid and sodium alginate to undergo esterification reaction for cross-linking, and then further solidifies under the action of calcium chloride to form immobilized microspheres, and forms hydrogen bond complexation and fixation with the composite biological enzyme through hydroxyl groups, thereby preparing the biological enzyme immobilized microspheres, which can not only effectively avoid the influence of alkaline conditions on the biological enzyme activity, but also improve the stability of the prepared laundry detergent, extend the shelf life, and have good washing effect, and have broad application prospects. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1

[0021] This embodiment provides a bio-enzyme laundry detergent.

[0022] Raw material composition (parts by weight): 7 parts of bio-enzyme immobilized microspheres, 10 parts of surfactants, 100 parts of deionized water, 0.1 parts of methylisothiazolinone, and 0.15 parts of flavors. The surfactants include Tween-20 and Span-20, with a mass ratio of 12:7.

[0023] The preparation method of bio-enzyme immobilized microspheres is as follows:

[0024] S1. 10 g of sodium alginate was added to 200 mL of dimethylformamide, 3 g of glutaric acid and 0.1 g of concentrated sulfuric acid were added, the mixture was heated under reflux and stirred for 10 h, 500 mL of 2 wt% calcium chloride solution was added dropwise, the mixture was centrifuged, washed, and dried to obtain immobilized microspheres;

[0025] S2. Add 3 g of composite biological enzyme into water, add 10 g of immobilized microspheres, freeze-dry, and prepare bioenzyme immobilized microspheres; the composite biological enzyme includes trypsin, neutral protease, and α-lipase, with a mass ratio of 10:7:5.

[0026] The preparation method of the bio-enzyme laundry detergent comprises the following steps: mixing bio-enzyme immobilized microspheres, a surfactant, deionized water, methylisothiazolinone and essence for 30 minutes, discharging the mixture, and preparing the bio-enzyme laundry detergent.

[0027] Example 2

[0028] This embodiment provides a bio-enzyme laundry detergent.

[0029] Raw material composition (parts by weight): 12 parts of bio-enzyme immobilized microspheres, 15 parts of surfactants, 120 parts of deionized water, 0.2 parts of methylisothiazolinone, and 0.3 parts of flavors. The surfactants include Tween-40 and Span-40, with a mass ratio of 15:10.

[0030] The preparation method of bio-enzyme immobilized microspheres is as follows:

[0031] S1. 12 g of sodium alginate was added to 200 mL of dimethylformamide, 5 g of glutaric acid and 0.2 g of concentrated sulfuric acid were added, the mixture was heated under reflux with stirring for 12 h, 500 mL of 4 wt% calcium chloride solution was added dropwise, the mixture was centrifuged, washed, and dried to obtain immobilized microspheres;

[0032] S2. Add 5 g of composite biological enzyme into water, add 10 g of immobilized microspheres, freeze-dry, and prepare bioenzyme immobilized microspheres; the composite biological enzyme includes trypsin, neutral protease, and α-lipase, and the mass ratio is 10:7:5.

[0033] The preparation method of the bio-enzyme laundry detergent comprises the following steps: mixing bio-enzyme immobilized microspheres, a surfactant, deionized water, methylisothiazolinone and essence for 30 minutes, discharging the mixture, and preparing the bio-enzyme laundry detergent.

[0034] Example 3

[0035] This embodiment provides a bio-enzyme laundry detergent.

[0036] Raw material composition (parts by weight): 10 parts of bio-enzyme immobilized microspheres, 12 parts of surfactants, 110 parts of deionized water, 0.15 parts of methylisothiazolinone, and 0.2 parts of flavor. The surfactants include Tween-85 and Span-85, with a mass ratio of 13:8.

[0037] The preparation method of bio-enzyme immobilized microspheres is as follows:

[0038] S1. 11 g of sodium alginate was added to 200 mL of dimethylformamide, 4 g of glutaric acid and 0.15 g of concentrated sulfuric acid were added, the mixture was heated under reflux and stirred for 11 h, 500 mL of 3 wt% calcium chloride solution was added dropwise, the mixture was centrifuged, washed, and dried to obtain immobilized microspheres;

[0039] S2. Add 4 g of composite biological enzyme into water, add 10 g of immobilized microspheres, freeze-dry, and prepare bioenzyme immobilized microspheres; the composite biological enzyme includes trypsin, neutral protease, and α-lipase, with a mass ratio of 10:7:5.

[0040] The preparation method of the bio-enzyme laundry detergent comprises the following steps: mixing bio-enzyme immobilized microspheres, a surfactant, deionized water, methylisothiazolinone and essence for 30 minutes, discharging the mixture, and preparing the bio-enzyme laundry detergent.

[0041] Comparative Example 1

[0042] Compared with Example 3, the difference is that the bio-enzyme immobilized microspheres are replaced by an equal amount of composite bio-enzymes.

[0043] This embodiment provides a bio-enzyme laundry detergent.

[0044] Raw material composition (parts by weight): 10 parts of composite biological enzyme, 12 parts of surfactant, 110 parts of deionized water, 0.15 parts of methylisothiazolinone, and 0.2 parts of flavor. The surfactant includes Tween-85 and Span-85, with a mass ratio of 13:8. The composite biological enzyme includes trypsin, neutral protease, and α-lipase, with a mass ratio of 10:7:5.

[0045] The preparation method of the bio-enzyme laundry detergent comprises the following steps: mixing composite bio-enzyme, surfactant, deionized water, methylisothiazolinone and essence for 30 minutes, discharging the mixture, and preparing the bio-enzyme laundry detergent.

[0046] Comparative Example 2

[0047] Compared with Example 3, the difference is that the surfactant is a single Tween-85.

[0048] This embodiment provides a bio-enzyme laundry detergent.

[0049] Raw material composition (parts by weight): 10 parts of bio-enzyme immobilized microspheres, 12 parts of Tween-85, 110 parts of deionized water, 0.15 parts of methylisothiazolinone, and 0.2 parts of essence.

[0050] The preparation method of bio-enzyme immobilized microspheres is as follows:

[0051] S1. 11 g of sodium alginate was added to 200 mL of dimethylformamide, 4 g of glutaric acid and 0.15 g of concentrated sulfuric acid were added, the mixture was heated under reflux and stirred for 11 h, 500 mL of 3 wt% calcium chloride solution was added dropwise, the mixture was centrifuged, washed, and dried to obtain immobilized microspheres;

[0052] S2. Add 4 g of composite biological enzyme into water, add 10 g of immobilized microspheres, freeze-dry, and prepare bioenzyme immobilized microspheres; the composite biological enzyme includes trypsin, neutral protease, and α-lipase, with a mass ratio of 10:7:5.

[0053] The preparation method of the bio-enzyme laundry detergent comprises the following steps: mixing bio-enzyme immobilized microspheres, Tween-85, deionized water, methylisothiazolinone and essence for 30 minutes, discharging the materials, and preparing the bio-enzyme laundry detergent.

[0054] Comparative Example 3

[0055] Compared with Example 3, the difference is that the surfactant is a single Span-85.

[0056] This embodiment provides a bio-enzyme laundry detergent.

[0057] Raw material composition (parts by weight): 10 parts of bio-enzyme immobilized microspheres, 12 parts of Span-85, 110 parts of deionized water, 0.15 parts of methylisothiazolinone, and 0.2 parts of flavor.

[0058] The preparation method of bio-enzyme immobilized microspheres is as follows:

[0059] S1. 11 g of sodium alginate was added to 200 mL of dimethylformamide, 4 g of glutaric acid and 0.15 g of concentrated sulfuric acid were added, the mixture was heated under reflux and stirred for 11 h, 500 mL of 3 wt% calcium chloride solution was added dropwise, the mixture was centrifuged, washed, and dried to obtain immobilized microspheres;

[0060] S2. Add 4 g of composite biological enzyme into water, add 10 g of immobilized microspheres, freeze-dry, and prepare bioenzyme immobilized microspheres; the composite biological enzyme includes trypsin, neutral protease, and α-lipase, with a mass ratio of 10:7:5.

[0061] The preparation method of the bio-enzyme laundry detergent comprises the following steps: mixing bio-enzyme immobilized microspheres, Span-85, deionized water, methylisothiazolinone and fragrance for 30 minutes, discharging the materials, and preparing the bio-enzyme laundry detergent.

[0062] Test Example 1

[0063] The bio-enzyme laundry detergents prepared in Examples 1-3 of the present invention and Comparative Examples 1-3 were tested for detergency and anti-redeposition ability according to the national standard B / T13174-2003 for detergents for clothing.

[0064] The results are shown in Table 1.

[0065] Table 1

[0066]

[0067]

[0068] It can be seen from the above table that the bio-enzyme laundry detergents prepared in Examples 1-3 of the present invention have better washing effects.

[0069] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A bio-enzyme laundry detergent, characterized in that: The invention is prepared from the following raw materials in parts by weight: 7-12 parts of bio-enzyme immobilized microspheres, 10-15 parts of surfactants, 100-120 parts of deionized water, 0.1-0.2 parts of preservatives and 0.15-0.3 parts of essences.

2. The bio-enzyme laundry detergent according to claim 1, characterized in that: The surfactant includes a hydrophilic emulsifier and a lipophilic emulsifier, and the mass ratio is 12-15:7-10.

3. The bio-enzyme laundry detergent according to claim 2, characterized in that: The hydrophilic emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85.

4. The bio-enzyme laundry detergent according to claim 2, characterized in that: The lipophilic emulsifier is selected from at least one of Span-20, Span-40, Span-60, Span-80 and Span-85.

5. The bio-enzyme laundry detergent according to claim 1, characterized in that: The preparation method of the bio-enzyme immobilized microspheres is as follows: S1. Add sodium alginate to dimethylformamide, add glutaric acid and a catalyst, heat to reflux and stir to react, add calcium chloride solution dropwise, centrifuge, wash, and dry to obtain immobilized microspheres; S2. Add the composite bio-enzyme into water, add the immobilized microspheres, and freeze-dry to obtain the bio-enzyme immobilized microspheres.

6. The bio-enzyme laundry detergent according to claim 5, characterized in that: The mass ratio of sodium alginate, glutaric acid and catalyst in step S1 is 10-12:3-5:0.1-0.

2.

7. The bio-enzyme laundry detergent according to claim 5, characterized in that: The concentration of the calcium chloride solution in step S1 is 2-4wt%, the catalyst is concentrated sulfuric acid, and the stirring reaction time is 10-12h.

8. The bio-enzyme laundry detergent according to claim 5, characterized in that: The composite biological enzyme in step S2 is one or both of protease and lipase.

9. The bio-enzyme laundry detergent according to claim 5, characterized in that: The mass ratio of the composite biological enzyme to the immobilized microspheres in step S2 is 3-5:

10.

10. A method for preparing the bio-enzyme laundry detergent according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: mixing biological enzyme immobilized microspheres, surfactant, deionized water, preservative and essence evenly, discharging the materials, and preparing biological enzyme laundry detergent.

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