Method for rapidly propagating rodgersia aesculifolia and rodgersia southwest rodgersia aesculifolia

Abstract

The invention provides a rapid propagation method for rodgersia aesculifolia and rodgersia aesculifolia. By controlling a cut block wound surface treatment mode, treatment time, cut block length, cut block thickness, embedding matrix humidity and illumination conditions, cut blocks are induced to bud, and transplanting and field planting are carried out. In the whole cultivation process, phytotoxicity, poison and other chemical influences are not generated on a cultivation medium and seedlings, a positive effect is achieved in the utilization process of important medicinal plants, and the method has good development, utilization, popularization and application prospects in the aspect of utilization of similar plants and is pioneered in China.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and more particularly to a method for rapid propagation of Coprinus pinnata and Coprinus fasciata. Background Art

[0002] Rodgersia is a perennial herbaceous plant of the Saxifragaceae family, mainly produced in the mountains of southwest my country. There are 5 species in the world (excluding subspecies), 4 of which are produced in China. As representative species of this genus, R. pinnata and R. sambucifolia are produced in Sichuan, Guizhou and Yunnan provinces in my country. Their habitats are mostly humid environments on the slopes at an altitude of 1,800-3,000 meters, and areas with a certain degree of shade under forests and by the water. R. pinnata and R. sambucifolia usually have horizontal rhizomes, which are also the main parts of the species used as medicine. As an important ethnic medicine, the feather-leaved Coleus chinensis and the southwestern Coleus chinensis are commonly used by the Naxi, Bai, Lisu and Yi peoples in my country. The common name of the medicine is generally "Yantuo". It contains anthraquinones, cardiac glycosides, tannins, etc. It tastes bitter and cold in nature. It has the effects of promoting blood circulation and regulating menstruation, dispelling wind and dampness, astringing and stopping diarrhea. It is mainly used to treat traumatic injuries, fractures, irregular menstruation, dysmenorrhea, rheumatic pain, traumatic bleeding, enteritis, dysentery and other diseases. In addition to its medicinal effects, the rhizomes of the feather-leaved Coleus chinensis and the southwestern Coleus chinensis are usually high in starch content, with a starch content of more than 18% in fresh products and up to 50% after drying. They can be used to make wine, vinegar and soy sauce. Its leaves contain a lot of tannins and can be made into tannin extract. In addition, the extracts of the feather-leaved Coleus chinensis and the southwestern Coleus chinensis (10% leaching) also have broad-spectrum antiviral effects and can be used as good source plants for the preparation of antiviral drugs.

[0003] At present, as a medicinal resource plant, except for its medicinal value included in local chronicles such as "Yunnan Medicine Record", "Dehong Medicine Record", "Yunnan Province Record", "Demin Record", and "Dali Capital Record", there has been no report on the rapid breeding technology of Featherleaf Ghost Lantern and Southwestern Ghost Lantern. However, in the southwest of my country, which is its main distribution area, folk use has long relied on the excavation of wild resources. With the increase in excavation intensity and scale year by year, its wild resources have sharply decreased. In Lijiang City, Yunnan Province, where Featherleaf Ghost Lantern and Southwestern Ghost Lantern are widely distributed, the wild population has decreased year by year in the nearly 20 years from 2000 to 2020, especially in areas with low altitudes and convenient transportation, where its wild population has decreased by 30%-40%. As the population decreases, the available rhizomes excavated are also getting smaller year by year, and the size of the rhizomes has decreased by more than 50%. In addition, under natural conditions, the pollination and fruit setting rates of this genus are extremely low, and the seed abortion phenomenon is very serious. Based on the results of previous surveys, the fruiting rate of wild populations is only about 50%. However, as the age structure of the population is getting younger (adult plants are being harvested in large numbers), the fruiting rate of most populations is less than 30%. It is urgent to carry out rapid reproduction and protection of the feather-leaved witch hazel and the southwestern witch hazel.

[0004] Therefore, finding more efficient planting methods to carry out rapid breeding of Psoraleafella and Psoraleafella can protect resources, meet the needs of returning to their native habitats, and alleviate the contradiction between supply and demand in the medicinal materials market. Summary of the invention

[0005] In order to solve or partially solve the problems existing in the related art, the present invention application provides a method for rapid propagation of Coprinus pinnata and Coprinus styraciflua.

[0006] The present application provides a method for rapid propagation of Coprinus pinnata and Coprinus serrata, the method comprising the following steps:

[0007] S1: Select the plants of Coprinus pinnatifida or Coprinus styraciflua with a rhizome length of 18-22 cm and an average diameter of 2 cm, remove the branches, leaves and fibrous roots, cut the rhizome perpendicular to the growth direction to obtain a block with a length of 3-5 cm, use a mixed powder to astringe the wound of the block within 2 hours, place it in a cool place, and cover the surface of the block with moist leaf mold; the mixed powder comprises talcum powder and sulfur powder;

[0008] S2: The cut pieces are buried in vermiculite, and the volume moisture content of the buried area is maintained at 50%-60%. After 45-50 days, budding individuals are obtained.

[0009] S3: Take out the budding individuals in S2, transplant them individually into a sterilized leaf mold substrate, and shade them with a shading degree of 75% until they become seedlings.

[0010] Furthermore, the volume ratio of talcum powder to sulfur powder in S1 is 20:1, and the particles of the mixed powder need to pass through a 500-mesh sieve.

[0011] Furthermore, the average diameter of the rhizome cut pieces in S1 is not less than 2 cm.

[0012] Furthermore, the embedding depth in S2 is such that the cut pieces are no more than 1 cm from the surface of the vermiculite and are kept from being exposed.

[0013] Furthermore, a plastic mesh frame with water permeability on all sides needs to be used in S2, and water should be replenished daily.

[0014] Furthermore, the germination condition in S2 is full shading.

[0015] Furthermore, the medium sterilized leaf mold matrix is ​​leaf mold that has passed through a 4-mesh sieve and has a volume moisture content of 50%-60%.

[0016] Furthermore, the particle size of the vermiculite is 1-3 mm.

[0017] Furthermore, the spacing between rows and plants was maintained at 20-25 cm during transplanting in S3.

[0018] Beneficial technical effects of the present invention:

[0019] 1. The method provided by the present invention is for asexual reproduction of rhizomes of P. pinnata and P. philadelphica by cutting. Since the obtained cuttings are of suitable physiological age, they can easily form adult plants in the later development and growth, thus shortening the years of cultivation.

[0020] 2. The method provided by the present invention mainly provides a detailed description and precise condition control of the rhizome cutting method, cutting size and cutting processing of the pinnate-leaved Coprinus japonica and the southwestern Coprinus japonica, inducing the formation of multi-bud individuals. In addition, the entire method does not use harmful substances such as chemical agents, and does not cause phytotoxicity, poisoning and other chemical effects on the cultivation medium and seedlings, which plays a positive role in the utilization of the plants as important medicinal plants.

[0021] 3. The method provided by the present invention is relatively simple to operate, and systematically explains the characteristics of cutting and propagating of P. pinnata and P. southwesternensis, and has good prospects for development, utilization and promotion of other plants in this genus. DETAILED DESCRIPTION

[0022] The optional embodiments of the present invention will be described in more detail below. Although the optional embodiments of the present invention are described, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0023] For the sake of clarity, the following is described in detail by the following Example 1. Unless otherwise specified, the test materials and reagents used in the following Example 1 are purchased from the market.

[0024] Example 1

[0025] A method for rapid propagation of Coprinus pinnata and Coprinus fasciata, comprising the following steps:

[0026] (1) In 2020 and 2021, live plants of P. lanceolata and P. styracifolia were collected from the vicinity of Yulong Snow Mountain in Lijiang City, Yunnan Province, and cultivated in the planting base of Lijiang Forestry Science Research Institute. After a growth cycle of not less than 2 years, in 2023, P. lanceolata and P. styracifolia plants with a rhizome length of 18-22 cm and an average diameter of 2 cm were selected. After removing branches, leaves and fibrous roots, the plants were cut into 3 cm blocks perpendicular to the growth direction of the rhizome, and 50 blocks were grouped into 1 group. The wounds were immediately treated (e.g., sprayed, dipped or smeared) with a mixed powder of talc powder and sulfur powder with a volume ratio of 20:1 to astringe the wounds, and placed in a cool place without direct sunlight for use, and the surface was covered with moist leaf mold to keep it moist.

[0027] (2) The cut pieces are buried in 1-3 mm particle vermiculite, with the depth of the cut pieces not exceeding 1 cm from the substrate surface, and they are kept out of the ground. The burying container is a plastic mesh frame with water permeability on all sides (specifications: length × width × height = 50 cm × 35 cm × 5 cm), and water is replenished daily to maintain its volume moisture content at 50%-60%. The plastic mesh frame is placed in a dark room (under complete darkness) and maintained at a room temperature of 15-25°C until the cut pieces sprout.

[0028] (3) Remove the pieces that have formed buds and transplant them individually into a seedbed filled with sterilized leaf mold that has passed a 4-mesh sieve, keeping the row spacing between plants at 20-25 cm. Water the substrate to maintain a volume moisture content of 50%-60%, and provide 75% shade until the seedlings are mature and then transplant them under the forest.

[0029] Comparative Example 1

[0030] According to the method for rapid propagation of Coprinus pinnata and Coprinus styraciflua provided in Example 1, in step (1), the ratio of talcum powder to sulfur powder is adjusted to 5:1, 10:1, 50:1 or without sulfur powder, and wound astringency is performed to determine the effectiveness of sulfur powder in wound treatment. Then, the steps (2) and (3) of Example 1 are implemented.

[0031] Table 1 Effects of different wound treatment methods on the survival rate of cut pieces of P. pelargonifolia and P. pelargonifolia

[0032]

[0033] As shown in Table 1, compared with Example 1 and Control Example 1, in the process of wound treatment of cut pieces of Coprinus lanceolata and Coprinus lanceolata, due to the high starch content of their rhizomes, they are very susceptible to microbial infection. The combination of talcum powder and sulfur powder is necessary for wound treatment, which can effectively reduce the infection rate of the cut pieces and ensure the budding and survival in the later stage. The compounding method of talcum powder and sulfur powder with a volume ratio of 20:1 should be the optimal ratio, which not only ensures the sterilization effect, but also minimizes the amount of sulfur powder and saves costs.

[0034] Comparative Example 2

[0035] According to the method for rapid propagation of Coprinus pinnata and Coprinus styraciflua provided in Example 1, in step (1), the time interval for treating the cut wound is adjusted, the cut wound is placed in an environment with an air humidity of 80%, the cut wound is treated after 1 hour, 2 hours, 8 hours and 24 hours, and the final seedling rate of the cut is observed. Then, the steps (2) and (3) of Example 1 are implemented.

[0036] Table 2 Effects of different wound treatment times on bud formation and bud number of cut pieces of P. pelargonifolia and P. cyrtonema

[0037]

[0038] As can be seen from Table 2, compared with Example 1 and Control Example 2, in the process of treating the cut wounds of Coprinus pinnata and Coprinus fasciata, immediate treatment of the wound is necessary, and should not be later than 2 hours at the latest. As the wound treatment of the cut is delayed, the dissolution and loss of nutrients in the rhizome will be caused. Although the total number of cuts for bud formation does not change much, it will greatly affect the number of buds in a single cut. The reduction of buds affects the synthesis and secretion of various growth hormones on the one hand, and also weakens the growth ability during the vegetative growth of the aboveground part on the other hand, which is unfavorable for later cultivation and planting.

[0039] Comparative Example 3

[0040] According to the method for rapid propagation of Coprinus pinnata and Coprinus styraciflua provided in Example 1, in step (1), the diameter of the cut pieces is controlled to be 2 cm, the length of the cut pieces is adjusted to be 1 cm, 2 cm, 4 cm, and 5 cm, and the relationship between the length of the cut pieces and the seedlings is determined. Then, the steps (2) and (3) of Example 1 are implemented.

[0041] Table 3 Effects of different cutting lengths on bud formation and number of buds in cuttings of Psoraleafella and Psoraleafella

[0042]

[0043] It can be seen from Table 3 that, compared with Example 1 and Control Example 3, when selecting the length of the cut pieces of Coprinus pinnata and Coprinus fasciata, they should be cut to at least about 3 cm to ensure effective budding of the cut pieces and the number of buds formed in a single cut piece.

[0044] Comparative Example 4

[0045] According to the method for rapid propagation of Coprinus pinnata and Coprinus styraciflua provided in Example 1, in step (1), the diameter of the cut pieces is adjusted to 1 cm, 1.5 cm, and 2.5 cm, and the relationship between the diameter of the cut pieces and the seedlings is determined. Then, the steps (2) and (3) of Example 1 are implemented.

[0046] Table 4 Effects of different cutting sizes on bud formation and bud number of cut pieces of P. pelargonifolia and P. cyrtonema

[0047]

[0048] Note: “\” represents unbudded, the same below.

[0049] As shown in Table 4, in Comparative Example 1 and Reference Example 4, when the thickness of the cut pieces of P. affine and P. affine was selected, the number of buds was the largest when the cut piece length was 2 cm. The growth of the rhizomes of P. affine and P. affine can usually reach 2 cm thick after 2-3 years of planting and cultivation, which ensures that the rhizomes have enough physiological age and can effectively sprout when cutting and propagating. The rhizomes with too young physiological age cannot sprout during the cutting and propagation process. And because the lateral growth rate of the rhizomes is faster, the thickening is slower. In order to obtain thicker rhizomes, longer growth years are often required, which is disadvantageous for rapid breeding and increases cost consumption.

[0050] Comparative Example 5

[0051] According to the method for rapid propagation of Coprinus pinnata and Coprinus styraciflua provided in Example 1, after implementing step (1) of Example 1, the volume moisture content of the substrate is controlled to be 30%-40%, 40%-50%, 60%-70%, 70%-80%, and the key humidity conditions for inducing buds from cuttings are determined. Then, step (3) of Example 1 is implemented.

[0052] Table 5 Effects of different germination substrate humidity on bud formation and bud number of cut pieces of Psoraleafella and Psoraleafella

[0053]

[0054] As shown in Table 5, compared with Example 1 and Comparative Example 5, 50%-60% is the most suitable humidity for embedding the cut pieces of Coleus pinnatus and Coleus styraciflua. Too high humidity will cause a large number of cut pieces to rot and die, or even fail to sprout; too low humidity will cause the cut pieces to sprout, but the number of sprouts from a single cut piece will show a significant downward trend, although the cut pieces can still sprout.

[0055] Comparative Example 6

[0056] According to the method for rapid propagation of Coprinus pinnata and Coprinus styraciflua provided in Example 1, after implementing step (1) of Example 1, the plastic mesh frame is placed under light intensity of 2000 lux, 24 h light (full light), 12 h light, and 12 h dark conditions to determine the effect of light induction on bud formation of cuttings. Then, step (3) of Example 1 is implemented.

[0057] Table 6 Effects of different germination substrate humidity on bud formation and bud number of cut pieces of Psoraleafella and Psoraleafella

[0058]

[0059] It can be seen from Table 6 that, compared with Example 1 and Control Example 6, after the cut pieces of Coprinus pinnata and Coprinus fasciata are embedded in the matrix, a completely dark environment should be selected for bud induction. Although light will not significantly affect the number of buds of a single cut piece, it will reduce the number of buds of the cut piece.

[0060] In summary, the method for rapid propagation of the feather-leaved Coprinus odoratus and the southwestern Coprinus odoratus provided by the present invention is relatively simple to operate, and the cuttings are induced to sprout and transplant and plant by controlling the cutting wound treatment method, treatment time, cutting length, thickness, embedding matrix humidity and lighting conditions. In the case where effective seed seedlings cannot be obtained for the feather-leaved Coprinus odoratus and the southwestern Coprinus odoratus, the reproductive capacity of the plant is improved, and because the physiological age of the cuttings is suitable, the growth can be quickly restored, shortening the cultivation and planting time. In the entire cultivation process, no chemical agents are used, so no drug damage, poisoning and other chemical effects are produced on the cultivation matrix and seedlings, which plays a positive role in the utilization process as an important medicinal plant, and has good development and utilization and promotion and application prospects in the utilization of similar plants, and is still the first in China.

[0061] Embodiment 1 of the present invention has been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiment 1. Many modifications and changes are obvious to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of terms used in this article is intended to best explain the principles of the embodiments, practical applications, or improvements to the technology in the market, or to enable other ordinary technicians in the art to understand the embodiments disclosed herein.

Claims

1. A method for rapid propagation of Coprinus pinnata and Coprinus fasciata, characterized in that: The method comprises the following steps: S1: Select a plant of Coprinus pinnatifida or Coprinus styraciflua with a rhizome length of 18-22 cm and an average diameter of 2 cm, remove branches, leaves and fibrous roots, cut the plant transversely perpendicular to the growth direction of the rhizome to obtain a block with a length of 3-5 cm, use a mixed powder to astringe the wound of the block within 2 hours, place the block in a cool place, and cover the surface of the block with moist leaf mold; the mixed powder comprises talcum powder and sulfur powder; S2: burying the cut pieces in vermiculite, maintaining the volume moisture content of the buried area at 50%-60%, and obtaining budding individuals after 45-50 days; S3: Take out the budding individuals in S2, transplant them individually into a sterilized leaf mold substrate, and shade them with a shading degree of 75% until they become seedlings.

2. The breeding method according to claim 1, characterized in that: The volume ratio of the talcum powder to the sulfur powder in S1 is 20:1, and the particles of the mixed powder need to pass through a 500-mesh sieve.

3. The breeding method according to claim 1, characterized in that: The average diameter of the rhizome pieces in S1 is not less than 2 cm.

4. The breeding method according to claim 1, characterized in that: The embedding depth of S2 is such that the cut pieces are no more than 1 cm away from the surface of the vermiculite and are kept from being exposed.

5. The breeding method according to claim 1, characterized in that: In the S2, a plastic mesh frame with water permeability on all sides should be used, and water should be replenished daily.

6. The breeding method according to claim 1, characterized in that: The germination conditions in S2 are full shading.

7. The breeding method according to claim 1, characterized in that: The sterilized leaf humus matrix in S3 is leaf humus that has passed through a 4-mesh sieve and has a volume moisture content of 50%-60%.

8. The breeding method according to claim 1, characterized in that: The vermiculite has a particle size of 1-3 mm.

9. The breeding method according to claim 1, characterized in that: During transplanting in S3, the spacing between rows and plants was maintained at 20-25 cm.

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