Low-temperature drying method for traditional Chinese medicine decoction pieces and compound decoction

Abstract

The invention provides a low-temperature drying method for traditional Chinese medicinal material decoction pieces, traditional Chinese medicinal materials are subsequent medicinal materials, and the low-temperature drying method comprises the following steps: 1) cutting fresh traditional Chinese medicinal materials into sections; preparing a protection solution; (2) soaking the cut traditional Chinese medicinal materials in the prepared protection liquid for 10-50 minutes; fishing out and naturally airing; 3) pre-freezing: pre-freezing the Chinese medicinal material sections in a freezing room at the temperature of-10 to-30 DEG C for 10-15 hours; and 4) freeze-drying: putting the pre-frozen Chinese medicinal material sections into freeze-drying equipment for freeze-drying. The invention also provides a compound decoction. According to the low-temperature drying method for the traditional Chinese medicinal decoction pieces, the following fresh traditional Chinese medicinal materials, especially the leaf fresh traditional Chinese medicinal materials, are treated with the protection liquid to keep effective components and keep leaves green, and then are subjected to low-temperature vacuum freezing, so that the effective components of the traditional Chinese medicinal decoction pieces are fully preserved.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese medicinal material processing, and particularly relates to a method for drying Chinese medicinal material slices and a decoction prepared by the method. Background Art

[0002] Vacuum freeze drying (freeze drying for short) is a drying method that first freezes the wet material to below the eutectic point to turn the water into solid ice, and then uses vacuum to directly sublimate the water in the material from solid to gas and discharge it out of the material.

[0003] Vacuum freeze drying technology is particularly popular due to its unique characteristics. This technology has been applied to the processing of some traditional Chinese medicine products, including precious herbs such as Rehmannia glutinosa, Gastrodia elata, Ginseng, Cordyceps sinensis and Ganoderma lucidum (Application and quality control of vacuum freeze drying in the processing of traditional Chinese medicine (English), Ren Difeng et al., Transactions of the Chinese Society of Agricultural Engineering, 2002, 18(5): 198-201.). In the study of the precious medicinal material Cordyceps sinensis, it was found that freeze drying and sun drying had a significant effect on the six main nucleosides and base components of Cordyceps sinensis. The total nucleosides, base components, hypoxanthine, xanthine, uridine, inosine and guanosine in the freeze-dried samples were higher than those in the sun-dried samples, accounting for the vast majority of the active ingredients, and the efficacy quality was better. Therefore, vacuum freeze drying is an important method and way to obtain high-quality medicinal materials (Research Progress in Drying Technology of Domestic Chinese Medicinal Materials and Raw Material Origin, Li Kunlun et al., Research and Promotion of Traditional Chinese Medicine / 2020, 2(4): 105-115).

[0004] Vacuum freeze drying has also been tried for woody Chinese medicinal materials. Patent application CN108635412A discloses a method for freshly extracting and freeze-drying woody Chinese medicinal materials into powder slices. After the medicinal materials are harvested, they are cleaned and selected to remove impurities such as mud and sand and non-medicinal parts, keep the epidermis moist, and cut into slices of 2 to 5 mm thick within 12 hours. Add 1 to 5 times the weight of the medicinal materials in 50 to 70°C water, soak and stir from time to time for 5 to 30 minutes, filter, and squeeze the residue to extract juice. Add 5 to 10 times the weight of the residue to the obtained residue, boil for 10 to 30 minutes, and filter while hot. Juice the residue again and collect the juice. Combine the above juices, pass through a 60 to 100 mesh sieve, and reduce the pressure at 50 to 80°C to a clear paste with a relative density of 1.10 to 1.25; keep 0.5% to 3% of the clear paste for use, and freeze the remaining clear paste and vacuum freeze-dry. The freeze-dried material is collected and mixed with the above-mentioned reserved clear paste to prepare granules, which are then dried, granulated and packaged to obtain the finished product.

[0005] Chinese herbal decoction refers to a liquid preparation obtained by decocting medicinal pieces or coarse granules with water or soaking them in boiling water, removing the residue and taking the juice. Its effective ingredients are well absorbed and take effect quickly. It is the most basic and most commonly used dosage form of Chinese herbal preparations. Chinese herbal decoctions often have limited pieces to be added later. When decocting the medicine, wait until the other medicinal materials are decocted before adding the medicinal materials added later, and fry them for a while. The medicinal materials added later mostly have volatile components and have a special aroma. Freeze-drying often leads to significant loss of many effective ingredients. Although low-temperature freezing technology has been widely used in the preparation of Chinese herbal pieces, how to keep the effective ingredients of leaf medicinal materials with volatile components from being lost is still a topic that requires continuous research and exploration by technical personnel in this field. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention proposes a low-temperature drying method for Chinese medicinal material slices of late-stage medicinal materials.

[0007] The second object of the present invention is to provide a compound decoction comprising the following Chinese medicinal materials:

[0008] The technical solution for achieving the above-mentioned purpose of the present invention is:

[0009] A method for low-temperature drying of Chinese herbal medicine slices, wherein the Chinese herbal medicine is a late-stage herbal medicine, and is dried by the following steps:

[0010] 1) Cut fresh Chinese medicinal materials into 1-5 cm segments; prepare a protective solution with a mass fraction of 5-8% glycerol, a mass fraction of 4-8% sodium chloride, a mass fraction of 0.05-0.1% chitosan oligosaccharide, a mass fraction of 2-8% glycerol, and a mass fraction of 0.5-1% glucose;

[0011] 2) Soak the cut Chinese medicinal materials in the prepared protective solution for 10 to 50 minutes; remove them and dry them naturally;

[0012] 3) Pre-freezing: Place the Chinese herbal medicine segments in a freezer at -10 to -30°C for 10 to 15 hours;

[0013] 4) Freeze drying: Place the pre-frozen Chinese medicinal material segments into a freeze drying device for freeze drying, set the freeze drying temperature to -20°C to -80°C, the vacuum degree to 30 to 80Pa, and the freeze drying time to 30 to 72 hours.

[0014] Wherein, the Chinese medicinal materials are selected from one or more of mint, patchouli, bitter melon, wormwood, perilla, mulberry leaf, artemisia annua, purslane, meconopsis, wormwood leaf and lophatherum gracile.

[0015] The protective liquid is prepared by: taking glycerol and water, placing them in a blender and mixing them thoroughly until they become emulsified to obtain glycerol emulsifiable concentrate;

[0016] Sodium chloride, chito oligosaccharide, glycerol and glucose are weighed and prepared according to the mass ratio; the mixture is placed in a blender and fully stirred and mixed with the glycerol emulsifiable concentrate to obtain a protective solution.

[0017] Preferably, in the protective solution, the mass fraction of glycerol is 5-6%, the mass fraction of sodium chloride is 4%-5%, the mass fraction of chitosan is 0.08-0.1%, the mass fraction of glucose is 0.4-0.6%, and the balance is water.

[0018] Chitosan oligosaccharide, also known as chitosan oligosaccharide or low-molecular-weight chitosan, is an oligosaccharide product with a degree of polymerization between 2 and 20 obtained by degrading chitosan by biological enzymes or chemical methods. Its molecular weight is ≤3200Da, and it is a low-molecular-weight product with high biological activity. Chitosan oligosaccharide has a high solubility, can be completely dissolved in water, and is easily absorbed and utilized by organisms. Chitosan oligosaccharide has an inhibitory effect on the growth of fungi and microorganisms, and is also used for the preservation and antibacterial preservation of fruits and vegetables.

[0019] Preferably, in step 4), the freeze-drying temperature is set at -40°C to -70°C, the vacuum degree is set at 40 to 70 Pa, and the freeze-drying time is set at 35 to 40 hours.

[0020] In the method, the Chinese herbal medicine slices are freeze-dried until the moisture content is reduced to below 5%.

[0021] The method further comprises the steps of:

[0022] 5) Crushing: crush the frozen medicinal materials into small pieces below 200 mesh.

[0023] A compound decoction comprises Chinese herbal medicine slices of the latter type, wherein the Chinese herbal medicine slices of the latter type are low-temperature dried by the method of the invention.

[0024] The compound decoction is composed of the following Chinese medicinal pieces in parts by weight: 12 to 18 parts of bupleurum, 10 to 12 parts of poria, 12 to 16 parts of stir-fried atractylodes, 12 to 15 parts of capillaris, 14 to 18 parts of perilla leaves, 10 to 14 parts of patchouli, and 5 to 7 parts of mint, wherein perilla leaves, patchouli and mint are the latter Chinese medicinal pieces.

[0025] Wherein, the compound decoction is prepared by the following method:

[0026] 1) Cut fresh medicinal materials of basil stem, patchouli and mint into sections according to mass proportions, and mix the cut Chinese medicinal materials.

[0027] 2) Soaking in the protective solution for 10 to 50 minutes; taking out and drying naturally;

[0028] 3) Mix the dried medicinal materials of Poria cocos, Atractylodes lancea and Artemisia capillaris according to the mass ratio, cut into 1-5 cm segments, and mix with the segments of the medicinal materials of the latter category; pre-freeze in a freezer at -15 to -30°C for 12 hours,

[0029] 4) Freeze drying: Place the pre-frozen Chinese medicinal materials in freeze drying equipment for freeze drying, set the freeze drying temperature to -65°C to -80°C, the vacuum degree to 30 to 80Pa, and the freeze drying time to 20 to 40 hours.

[0030] Although studies have shown that non-post-treatment drugs can be frozen at lower temperatures and without vacuuming, for compound decoctions, mixing various medicinal materials can achieve better synergistic effects; in addition, the inventors also noticed that freezing the post-treatment drugs and other medicinal materials separately and then mixing them will cause local agglomeration problems, resulting in longer crushing time.

[0031] A further preferred technical solution of the present invention is:

[0032] 1) Cut fresh medicinal materials of perilla leaves, patchouli and mint into 1-5 cm segments; mix them according to mass fraction, and prepare a protective solution according to the following ratio: 5-6% mass fraction of glycerol, 4%-5% mass fraction of sodium chloride, 0.06-0.08% mass fraction of chitosan oligosaccharide, 0.6-0.8% mass fraction of glucose, and the balance is water;

[0033] 2) Soak the cut Chinese medicinal materials in the prepared protective solution for 30 minutes; take them out and dry them naturally;

[0034] 3) Pre-freezing: Take dried medicinal materials of Poria cocos, Atractylodes lancea and Artemisia capillaris according to the mass proportion, cut into 1-5 cm segments, mix with the medicinal materials of the latter category, and pre-freeze all the segments of the Chinese medicinal materials in a -18°C freezer for 12 hours;

[0035] 4) The pre-frozen medicinal material segments are placed in a freeze dryer for freeze drying. The freeze dryer is pre-cooled to about -65 to -70°C, the vacuum degree is set to 60 to 70 Pa, and the freeze drying time is 30 to 40 hours;

[0036] 5) Grinding: Mix the frozen small pieces of medicinal materials with the later-stage medicinal pieces and grind them to less than 200 mesh.

[0037] When patchouli, atractylodes and poria are used together, they can enhance the effects of removing dampness, strengthening the spleen and stomach, and can effectively improve symptoms such as edema, phlegm, fatigue, and loose stools due to spleen deficiency. Patients with pale tongue and white fur, who are diagnosed with wind-cold and dampness, can consider using this compound decoction.

[0038] Directions: Reconstitute with water. Add about 300 mL of warm water for every 85 grams of decoction granules.

[0039] The beneficial effects of the present invention are:

[0040] The low-temperature drying method for Chinese herbal medicine slices proposed by the present invention is aimed at fresh Chinese herbal medicines, especially leaves, which are first treated with a protective liquid to maintain effective ingredients and keep the leaves green, and then low-temperature vacuum frozen, so that the effective ingredients of the Chinese herbal medicine slices can be fully preserved;

[0041] The compound decoction prepared by the method has a particle size of less than 200 meshes and can be directly reconstituted with water without the need for decocting, thereby greatly saving manpower and material resources and providing convenience in storage, transportation, packaging and sales. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is the spectrum of ultra-high performance liquid chromatography tandem mass spectrometry of the sample in Example 5 of the present invention (in positive ion mode);

[0043] Figure 2 This is the spectrum of ultra-high performance liquid chromatography tandem mass spectrometry of the sample in Example 5 (in negative ion mode);

[0044] Figure 3 is the spectrum of ultra-high performance liquid chromatography tandem mass spectrometry of the comparative sample (in positive ion mode);

[0045] Figure 4 It is the spectrum of ultra-high performance liquid chromatography tandem mass spectrometry of the comparative sample (under negative ion mode). DETAILED DESCRIPTION

[0046] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0047] Unless otherwise specified, the technical means used in the specification are all known in the art. All raw materials used can be purchased commercially.

[0048] Embodiment 1: Low temperature drying method for Chinese herbal medicine slices

[0049] Fresh mint leaves were pre-frozen in a -40°C refrigerator for 12 hours and then placed in a freeze dryer for freeze drying. The freeze dryer was pre-cooled to about -80°C (i.e., freeze drying temperature) and the freeze drying lasted for 24 hours.

[0050] After freeze-drying, the sample was crushed into 200 mesh, and 150 mL of water was added to about 30 g of the sample. The sample was shaken thoroughly to dissolve and then analyzed by GC-MS.

[0051] Embodiment 2 Low temperature drying method of Chinese herbal medicine slices

[0052] Fresh mint leaves were pre-frozen in a -40℃ refrigerator for 12 hours, and then placed in a freeze dryer for freeze drying. The freeze dryer was pre-cooled to about -70℃, the vacuum was set to 80Pa, and the freeze drying time was 24 hours.

[0053] After freeze-drying, the sample was crushed into 200 mesh, and 150 mL of water was added to about 30 g of the sample. The sample was shaken thoroughly to dissolve and then analyzed by GC-MS.

[0054] After the samples of Example 1 and Example 2 were freeze-dried, the mint leaves were gray and lost most of their fragrance. The researchers of this project referred to existing antifreeze drugs, color preservatives, etc., and tried to prepare protective solutions. Sodium chloride is a known color preservative used to keep the leaves green; there are many agents used for plant antifreeze, but this research project is a method for preparing Chinese herbal medicine slices, and any drugs that bring in other chemical activities or destroy the biological activity of Chinese medicine itself cannot be used. Therefore, only sugars and glycerol were selected for testing.

[0055] Embodiment 3: Low temperature drying method for Chinese herbal medicine slices

[0056] 1) Take fresh mint leaves; and prepare protective solution:

[0057] Take 6 kg of glycerol and 88 kg of water, place them in a blender and mix thoroughly until they become milky to obtain glycerol emulsifiable concentrate;

[0058] Weigh the drugs according to the following proportions: 5% sodium chloride by mass, 1% glucose by mass, add them into the glycerol emulsion, and stir evenly;

[0059] 2) Soak the mint leaves in the prepared protective solution for 30 minutes; remove them and dry them naturally;

[0060] 3) Pre-freezing: Pre-freeze the Chinese herbal medicine segments in a -18°C freezer for 12 hours;

[0061] 4) Freeze drying: The pre-frozen small pieces of Chinese medicinal materials are placed in a freeze drying device for freeze drying. The freeze drying temperature is set to -68°C, the vacuum degree is set to 70Pa, the freeze drying time is 36h, and the freeze drying is performed until the moisture content is reduced to 5%.

[0062] After freeze-drying, the sample was crushed into 200 mesh, and 150 mL of water was added to about 30 g of the sample. The sample was shaken thoroughly to dissolve and then analyzed by GC-MS.

[0063] Embodiment 4: Low temperature drying method for Chinese herbal medicine slices

[0064] The components of the prepared protective solution are: 5% sodium chloride, 0.1% chitosan, 6% glycerol, 0.5% glucose, and the balance water.

[0065] Other operations are the same as those in Example 3.

[0066] The mint leaves in Example 3 and Example 4 remained basically unchanged in color after being frozen, and their fragrance was well preserved.

[0067] This research project uses mint as a representative of leafy medicinal materials with volatile components. Fresh mint leaves were tested by cryogenic freezing, cryogenic vacuum freezing, and cryogenic vacuum freezing with a protective agent. After freezing, the appearance and smell were observed, and gas chromatography-mass spectrometry (GC-MS) analysis was performed: the GC headspace injection bottle was placed in the GC instrument and the direct headspace injection method was used for detection. The main components were identified by GC-MS, and the peak areas of the identified components were compared.

[0068] Sample preparation for unfreeze-dried Chinese herbal medicines: Take 300 mL of water, boil it, add 30 g of mint, and boil until the volume is reduced by about half. Use a 200-mesh sieve to filter the extract and let it cool to room temperature. Take 2 mL of the extract and put it into a GC headspace injection bottle for gas chromatography-mass spectrometry analysis.

[0069] GC-MS identified two representative components, L-menthone and dl-menthol. The molecular formula of L-menthone is C 10 H 18 O, retention time 18.1min; dl-menthol molecular formula C 10 H 20 O, retention time 18.6min, GC-MS identification results are shown in the table below. The test results show that if freeze-drying is performed without adding a protective agent, the peak area of ​​each component in the freeze-dried group is smaller than that in the non-freeze-dried group. Although p>0.05, the overall trend of volatile components decreasing is more obvious. Soaking in a protective solution and then freeze-drying has a significant protective effect on the representative components. Among them, the effect of Example 4 is better. The optimized freezing method is also used in the preparation of compound decoctions.

[0070] Table 1: Components and peak areas detected by GC (n=3)

[0071]

[0072] Embodiment 5 A kind of compound decoction

[0073] The present embodiment proposes a compound decoction, which is composed of the following Chinese herbal medicine pieces in parts by weight: 15 parts of bupleurum, 10 parts of poria, 15 parts of stir-fried atractylodes, 15 parts of capillaris, 15 parts of perilla leaves, 10 parts of patchouli, and 5 parts of mint, among which perilla leaves, patchouli and mint are the latter Chinese herbal medicine pieces.

[0074] The preparation method of this compound decoction is as follows:

[0075] 1) Cut fresh medicinal materials of perilla leaf, patchouli and mint into segments of 1 to 5 cm; mix them according to mass proportions, and prepare a protective solution according to the proportions of Example 4.

[0076] 2) Soak the cut Chinese medicinal materials in the prepared protective solution for 30 minutes; take them out and dry them naturally;

[0077] 3) Pre-freezing: Mix the dried medicinal materials of Poria cocos, Atractylodes lancea and Artemisia capillaris according to the mass proportion, cut into 1-5 cm segments, mix with the medicinal materials of the latter category, and pre-freeze all the segments of the Chinese medicinal materials in a -18°C freezer for 12 hours;

[0078] 4) placing the pre-frozen Chinese medicinal material segments into a freeze-drying device for freeze drying, setting the freeze drying temperature to -68°C, the vacuum degree to 70Pa, the freeze drying time to 36h, and freeze drying until the moisture content is reduced to less than 5%;

[0079] 5) Crushing: crush the frozen medicinal material slices to less than 200 mesh.

[0080] Directions: Reconstitute with water. Add about 300 mL of warm water for every 85 grams of decoction granules.

[0081] Comparative Example:

[0082] 15 parts of Bupleurum, 10 parts of Poria, 15 parts of stir-fried Atractylodes, 15 parts of Artemisia Capillaris, according to 85 grams of Chinese herbal medicine pieces (including the latter ones), soak in 760 mL of water for 30 minutes; after decocting for 25 minutes, add 15 g of Perilla leaves, 10 parts of Patchouli, and 5 parts of mint, continue to decoct for 5 minutes, filter the extract through a 100-mesh sieve, add 608 mL of water for the second time, decoct for 30 minutes, filter through a 100-mesh sieve, and combine the two extracts.

[0083] The decoction was concentrated to about 300 mL.

[0084] 100 μL of each sample from Example 5 and Comparative Example was extracted twice with 800 μL of acetonitrile, and the supernatant was taken by centrifugation. The supernatant was put into a LABCONCO tabletop vacuum centrifugal concentrator to evaporate the solvent, and re-dissolved in 100 μL of acetonitrile for UPLC-MS detection.

[0085] UPLC-MS detection conditions:

[0086] Chromatographic column: Waters liquid chromatography column (130A, 5μm, 4.6×250mm), column temperature: 30°C, injection volume: 2μL, flow rate: 0.3mL / min, mobile phase: A (acetonitrile, containing 0.1% formic acid); B (deionized water, containing 0.1% formic acid and 10mM ammonium acetate), gradient elution: 0-4min, 0% B; 4-6min, 0%-25% B; 6-15min, 25% B; 15-16min, 25-50% B; 16-25min, 50% B; 25-26min, 0% B; 26-30min, 0% B.

[0087] Ion source: sheath gas flow rate 40arb, auxiliary gas flow rate 15arb, capillary temperature 300℃, auxiliary gas heater temperature 330℃, positive spray voltage 3.2kv, negative spray voltage 3.0kv. The resolution of MS is 70000, the resolution of MS / MS is 17500, the scanning mode is full scan mode, positive and negative ion modes are detected simultaneously, and the mass spectrometry scanning range is m / z 75-1000.

[0088] Results Figures 1 to 4 Patchouli ketone, linoleic acid, ferulic acid, apigenin and 7-methoxycoumarin were detected, among which patchouli ketone was the main active ingredient in patchouli volatile oil. There was no significant difference in the peak area of ​​the same component between the two groups.

[0089] Although the present invention has been described above through embodiments, those skilled in the art should understand that without departing from the spirit and substance of the present invention, all improvements and modifications made to the present invention should fall within the protection scope of the present invention.

Claims

1. A method for low-temperature drying of Chinese herbal medicine slices, wherein the Chinese herbal medicine is a late-stage herbal medicine, characterized in that: Drying is done by: 1) Cut fresh Chinese medicinal materials into 1-5 cm segments; prepare a protective solution with a mass fraction of 5-8% glycerol, a mass fraction of 4-8% sodium chloride, a mass fraction of 0.05-0.1% chitosan oligosaccharide, a mass fraction of 2-8% glycerol, and a mass fraction of 0.5-1% glucose; 2) Soak the cut Chinese medicinal materials in the prepared protective solution for 10 to 50 minutes; remove them and dry them naturally; 3) Pre-freezing: Place the Chinese herbal medicine segments in a freezer at -10 to -30°C for 10 to 15 hours; 4) Freeze drying: Place the pre-frozen Chinese medicinal material segments into a freeze drying device for freeze drying, set the freeze drying temperature to -20°C to -80°C, the vacuum degree to 30 to 80Pa, and the freeze drying time to 30 to 72 hours.

2. The method for low-temperature drying of Chinese herbal medicine slices according to claim 1, characterized in that: The Chinese medicinal materials are selected from one or more of mint, patchouli, bitter melon, wormwood, perilla, mulberry leaf, artemisia annua, purslane, meconopsis, wormwood leaf and lophatherum gracile.

3. The method for low-temperature drying of Chinese herbal medicine slices according to claim 1, characterized in that: The protective liquid is prepared by: taking glycerol and water, placing them in a blender and mixing them thoroughly until they are emulsified to obtain glycerol emulsifiable concentrate; Sodium chloride, chito oligosaccharide, glycerol and glucose are weighed and prepared according to the mass ratio; the mixture is placed in a blender and fully stirred and mixed with the glycerol emulsifiable concentrate to obtain a protective solution.

4. The method for low-temperature drying of Chinese herbal medicine slices according to claim 1, characterized in that: In the protective solution, the mass fraction of glycerol is 5-6%, the mass fraction of sodium chloride is 4-5%, the mass fraction of chitosan is 0.08-0.1%, the mass fraction of glucose is 0.4-0.6%, and the balance is water.

5. The method for low-temperature drying of Chinese herbal medicine slices according to any one of claims 1 to 4, characterized in that: In step 4), the freeze-drying temperature is set at -40°C to -70°C, the vacuum degree is set at 40 to 70 Pa, and the freeze-drying time is set at 35 to 40 hours.

6. The method for low temperature drying of Chinese herbal medicine slices according to any one of claims 1 to 4, characterized in that: Also includes the steps: 5) Crushing: crush the frozen medicinal materials into small pieces below 200 mesh.

7. A compound decoction comprising Chinese medicinal pieces of the following type, characterized in that: The Chinese medicinal pieces of the latter type are low-temperature dried by the method described in any one of claims 1 to 6.

8. The compound decoction according to claim 7, characterized in that: The medicine is composed of the following Chinese medicinal pieces in parts by weight: 12-18 parts of bupleurum, 10-12 parts of poria, 12-16 parts of stir-fried atractylodes, 12-15 parts of capillaris, 14-18 parts of perilla leaves, 10-14 parts of patchouli, and 5-7 parts of mint, among which perilla leaves, patchouli and mint are the latter Chinese medicinal pieces.

9. The compound decoction according to claim 8, characterized in that: The compound decoction is prepared by the following method: 1) Cut fresh medicinal materials of basil stem, patchouli and mint into sections according to mass proportions, and mix the cut Chinese medicinal materials. 2) Soaking in the protective solution for 10 to 50 minutes; taking out and drying naturally; 3) Mix the dried medicinal materials of Poria cocos, Atractylodes lancea and Artemisia capillaris according to the mass ratio, cut into 1-5 cm segments, and mix with the segments of the medicinal materials of the latter category; pre-freeze in a freezer at -15 to -30°C for 12 hours, 4) Freeze drying: Place the pre-frozen Chinese medicinal materials in freeze drying equipment for freeze drying, set the freeze drying temperature to -65°C to -80°C, the vacuum degree to 30 to 80Pa, and the freeze drying time to 20 to 40 hours.

10. The compound decoction according to any one of claims 7 to 9, characterized in that: Prepared by the following steps: 1) Cut fresh medicinal materials of perilla leaves, patchouli and mint into 1-5 cm segments; mix them according to mass fraction, and prepare a protective solution according to the following ratio: 5-6% mass fraction of glycerol, 4%-5% mass fraction of sodium chloride, 0.06-0.08% mass fraction of chitosan oligosaccharide, 0.6-0.8% mass fraction of glucose, and the balance is water; 2) Soak the cut Chinese medicinal materials in the prepared protective solution for 30 minutes; take them out and dry them naturally; 3) Pre-freezing: Take dried medicinal materials of Poria cocos, Atractylodes lancea and Artemisia capillaris according to the mass proportion, cut into 1-5 cm segments, mix with the medicinal materials of the latter category, and pre-freeze all the segments of the Chinese medicinal materials in a -18°C freezer for 12 hours; 4) The pre-frozen medicinal material segments are placed in a freeze dryer for freeze drying. The freeze dryer is pre-cooled to about -65 to -70°C, the vacuum degree is set to 60 to 70 Pa, and the freeze drying time is 30 to 40 hours; 5) Grinding: Mix the frozen small pieces of medicinal materials with the later-stage medicinal pieces and grind them to less than 200 mesh.

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