Biological tissue paraffin embedding and slicing all-in-one machine

Abstract

The invention provides a biological tissue paraffin embedding and slicing all-in-one machine, and relates to the technical field of embedding and slicing, the biological tissue paraffin embedding and slicing all-in-one machine comprises a base, the base is provided with an embedding mechanism facilitating paraffin embedding of tissues, a fixing mechanism facilitating slicing of paraffin blocks, and a cutting mechanism facilitating slicing of the paraffin blocks; the embedding mechanism comprises a transmission frame, a metal box, a movable rod and a liquid storage tank, the fixing mechanism comprises an adjusting frame and an adjusting plate, and the cutting mechanism comprises a mounting block, a push plate and a slicing knife. According to the device, the adjusting plate and the fixing frame which can work alternately are arranged to be matched with each other, so that the processes of loading, slicing, disassembling and the like are carried out in parallel, and the time waste caused by the fact that the next process can be carried out after one process is completed in the existing mode is avoided; for a laboratory or a production environment needing a large number of slices and loading slices, the overall operation time is greatly shortened, and the slicing efficiency is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of embedding and slicing, and more specifically, particularly relates to an integrated machine for embedding and slicing biological tissues using paraffin wax. Background Art

[0002] Paraffin embedding and sectioning is a commonly used histological technique used to prepare biological tissue samples for observation and analysis under a microscope. Paraffin sections are not only used to observe the morphological structure of normal cell tissues, but are also the main method used by disciplines such as pathology and forensic medicine to study, observe and judge the morphological changes of cell tissues.

[0003] The Chinese patent publication number is: CN113670694A, a biological tissue paraffin embedding and slicing integrated machine, the invention can automatically perform paraffin embedding, cooling, and slicing, without the need for multiple manual transfers, which can improve the operating efficiency, while reducing the work intensity of workers, and is convenient and fast; in order to prevent the wax liquid from overflowing onto the conveyor belt during unloading, a wax box is arranged outside the embedding box to hold the overflow wax, and a support block is arranged between the bottom of the embedding box and the wax box, which can prevent the embedding box from sticking to the wax box after the wax overflows, facilitate the removal of the embedding box, and effectively improve its functionality and practicality.

[0004] Existing embedding and slicing equipment has the following disadvantages:

[0005] 1. When controlling the wax liquid conflict, the existing equipment mostly uses manual operation of valves for processing. The wax liquid usually remains in liquid state at a higher temperature. When manually operating the valve, it is easy to come into contact with the high-temperature wax liquid, which poses a risk of scalding. It is difficult to accurately control the flow rate and dripping time of the wax liquid by manual control. In addition, the existing wax outlet has a fixed height, and the wax liquid may splash when discharging wax;

[0006] 2. The existing equipment has only a single workbench. During the actual slicing operation, the next round of operation can only be carried out after the loading and unloading processes are completed. It is impossible to carry out the processes in parallel, which reduces work efficiency. In addition, the single operating table requires operators to switch frequently between different processes, which is prone to confusion and incoordination, affecting work efficiency.

[0007] Therefore, in view of this, the existing structure and defects are studied and improved, and a biological tissue paraffin embedding and slicing integrated machine is provided, in order to achieve a purpose with greater practical value. Summary of the invention

[0008] In order to solve the above technical problems, the present invention provides a biological tissue paraffin embedding and slicing integrated machine to solve the above problems.

[0009] A biological tissue paraffin embedding and slicing integrated machine comprises a base, the base is provided with an embedding mechanism for facilitating paraffin embedding of tissues, the base is provided with a fixing mechanism for facilitating slicing of wax blocks, the base is provided with a cutting mechanism for facilitating slicing of wax blocks, the embedding mechanism comprises a transmission frame, a metal box, a movable rod and a liquid storage tank, the fixing mechanism comprises an adjustment frame and an adjustment plate, the cutting mechanism comprises a mounting block, a push plate and a slicing knife, the transmission frame is fixedly mounted on the upper end of the base, an L-shaped frame is fixedly mounted on the upper end of the metal box, the liquid storage tank is fixedly mounted on the inner side wall of the L-shaped frame, the movable rod is located at the side end of the transmission frame, the adjustment frame is fixedly mounted on the side end of the base, and the side end of the adjustment frame is penetrated by a circular opening The cam is provided with a plurality of adjustment plates, each of which is fixedly mounted on the circumferential end of the movable shaft at an equal distance. The mounting block is fixedly mounted on the upper end of the base. The upper end of the mounting block is fixedly mounted on a cylinder. The upper end of the cylinder is fixedly mounted on a frame. Two push rods are fixedly mounted on the side ends of the push plate. A T-shaped plate is fixedly mounted on the side ends of the push plate. Two fifth telescopic rods are fixedly mounted between the side ends of the T-shaped plate and the frame. A cooling box is fixedly mounted on the upper end of the transmission frame. A valve is fixedly mounted on the lower end of the liquid storage tank. A bellows is fixedly mounted on the lower end of the valve. A valve plate is provided on the inner side wall of the valve. A rotating Axle, a first gear is fixedly installed on the circumferential end of the rotating shaft, the rotating shaft passes through and is rotatably installed on the circumferential end of the valve, a sliding groove is passed through the side end of the transmission frame, a wedge is fixedly installed on the side end of the movable rod, and the wedge passes through and is slidably installed on the inner side wall of the sliding groove, two first telescopic rods are fixedly installed between the movable rod and the transmission frame, and two first springs are also fixedly installed between the movable rod and the transmission frame, and the two first springs are respectively located on the inner side walls of the two first telescopic rods, two connecting pieces are fixedly installed on the lower end of the two connecting pieces, and two cross plates are fixedly installed on the upper ends of the two cross plates, and the racks are fixedly installed on the upper ends of the two cross plates, and the racks are meshed with the rotating shaft, and the side end of the adjusting frame passes through Four limiting grooves are opened, a pull rod is provided at the side end of the movable shaft, a second telescopic rod is fixedly installed between the pull rod and the movable shaft, a second spring is also fixedly installed between the pull rod and the movable shaft, the second telescopic rod is located on the inner side wall of the second spring, a limiting rod is fixedly installed on the side end of the pull rod, and the limiting rod is located on the inner side wall of the limiting groove, a fixing frame is fixedly installed on the side end of each adjustment plate, a bottom plate is fixedly installed on the lower end of each fixing frame, a plurality of C-shaped positioning plates are slidably installed on the end of each fixing frame, a third spring is fixedly installed between each C-shaped positioning plate and the bottom plate, a movable plate is also slidably installed on the inner side wall of the fixed frame, and two fourth springs are fixedly installed between the movable plate and the bottom plate.Two fourth telescopic rods are fixedly installed between the movable plate and the bottom plate, and the two fourth telescopic rods are respectively located on the inner side walls of the fourth spring. A side rod is fixedly installed on the side end of the frame, and a T-shaped slide groove is provided on the side end of the side rod. A turntable is provided on the side end of the frame, and a handle is fixedly installed on the side end of the turntable. The handle is rotatably installed on the side end of the frame, and a second gear is fixedly installed on the circumferential end of the handle. A first round rod is also fixedly installed on the side end of the frame.

[0010] Preferably, a threaded groove is penetrated through the side end portion of the T-shaped plate, a threaded rod is threadedly rotatably installed on the inner side wall of the threaded groove, the threaded rod is penetrated and rotatably installed on the side end portion of the frame, a first conical tooth is fixedly installed on the circumferential end portion of the threaded rod, a third gear is rotatably installed on the circumferential end portion of the first round rod, a toothed belt is provided between the third gear and the second gear, a second conical tooth is fixedly installed on the side end portion of the third gear, a second round rod is also penetrated and rotatably installed on the side end portion of the frame, a third conical tooth and a fourth conical tooth are fixedly installed on the circumferential end portion of the second round rod, the third conical tooth and the second conical tooth are meshed with each other, and the fourth conical tooth and the first conical tooth are meshed with each other.

[0011] Preferably, a T-shaped slide bar is fixedly mounted on the side end of the slicing knife, and the T-shaped slide bar is slidably mounted on the inner wall of the T-shaped slide groove. A second round protrusion is fixedly mounted on the side end of the T-shaped slide bar, and a transmission rod is rotatably mounted between the second round protrusion and the first round protrusion.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] In the present invention, by arranging the metal box, the movable rod and the valve plate to cooperate, when the metal box moves to the bottom of the bellows, the valve plate can be driven to open automatically, and when the metal box leaves the bottom of the bellows, the valve plate can be automatically driven to close, without the need for manual operation of the valve plate, reducing the time and steps of manual intervention, and ensuring that the wax liquid will drip only when the metal box is in the correct position each time, so that the amount of wax liquid can be accurately controlled to avoid affecting the embedding quality due to too much or too little wax liquid. When the metal box leaves the liquid outlet, the valve is automatically closed, which can effectively prevent the wax liquid from accidentally dripping and reduce the pollution to the working environment.

[0014] In the present invention, by providing a valve plate that opens and closes automatically, the opening and closing of the valve plate can be changed by changing the position of the metal box, thereby eliminating the need for manual adjustment of the valve plate. Since wax liquid usually remains in liquid form at a relatively high temperature, manual operation of the valve plate is likely to contact the high-temperature wax liquid, which poses a risk of scalding. However, the coordination of the movable rod, the rack and the first gear enables the valve plate to be opened and closed without manual contact or proximity to the valve area, thereby enabling operators to stay away from the high-temperature area and avoid scalding accidents.

[0015] In the present invention, by arranging the adjustment plate and the fixing frame that can work alternately, the parallel operation of the processes of loading, slicing, and disassembling is realized, which avoids the time waste of having to wait for one process to be completed before the next process can be carried out in the existing method. Multiple workbenches work alternately, which greatly shortens the overall operation time and improves the slicing efficiency for laboratories or production environments that require a large amount of slicing and loading.

[0016] In the present invention, by arranging the adjustment plates, the fixed frames and the movable shafts that are used alternately, the alternate work tables enable the operators to clearly divide the work, with one part focusing on the slicing operation and the other part responsible for the loading work. The clear division of labor can also reduce the switching time and error rate of the operators between different processes, improve the coordination and fluency of the overall work, enable the manpower in the laboratory to be flexibly allocated, and optimize the work efficiency.

[0017] In the present invention, the embedding mechanism and the slicing mechanism are coordinated to form an integrated design. The integrated design allows the tissue sample to be directly sliced ​​after embedding. Since the sample does not need to be moved between different devices, the risk of sample damage caused by transportation, collision, etc. is reduced, the intermediate operation steps and time waste are reduced, and the space utilization rate of the experimental environment is improved, ensuring work efficiency.

[0018] In the present invention, by arranging the threaded rod, the T-shaped plate, the push plate and the movable plate to cooperate with each other, when the handle is rotated to drive the slicer to move back and forth for cutting, the wax block on the embedding plate is driven to move at a uniform speed, so that the speed at which the wax block passes through the blade is constant during each cutting, which makes the thickness of the slices cut more uniform, avoids the problem of excessive difference in slice thickness caused by unstable movement speed of the wax block, ensures the slice quality while ensuring the slice efficiency, and has better stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the base of the present invention;

[0020] Figure 2 It is a structural schematic diagram of the transmission frame of the present invention;

[0021] Figure 3 It is a structural schematic diagram of the liquid storage tank of the present invention;

[0022] Figure 4 It is a schematic diagram of the structure of the bellows of the present invention;

[0023] Figure 5 It is a structural schematic diagram of the adjustment frame of the present invention;

[0024] Figure 6It is a structural schematic diagram of the fixing frame of the present invention;

[0025] Figure 7 It is a structural schematic diagram of the turntable of the present invention;

[0026] Figure 8 It is a structural schematic diagram of the push plate of the present invention;

[0027] Fig. 9 It is a structural schematic diagram of the toothed belt of the present invention.

[0028] In the figure, the corresponding relationship between the names of the components and the numbers of the accompanying drawings is: 1. Base; 11. Cooling box; 12. L-shaped stand; 13. Liquid storage box; 14. Valve; 15. Bellows; 16. Valve plate; 17. Rotating shaft; 18. First gear; 19. Slide; 2. Metal box; 21. Transmission frame; 3. Movable rod; 31. First spring; 32. First telescopic rod; 33. Wedge block; 34. Connecting piece; 35. Cross plate; 36. Rack; 4. Adjusting frame; 41. Limiting groove; 42. Circular groove; 43. Movable shaft; 44. Second spring; 45. Second telescopic rod; 46. Pull rod; 47. Limiting rod; 5. Adjusting plate; 51. Fixed frame; 52. Bottom plate; 53. C-shaped positioning plate ; 54. third spring; 55. movable plate; 56. fourth spring; 57. fourth telescopic rod; 6. mounting block; 61. cylinder; 62. frame; 63. side rod; 64. T-shaped slide; 65. turntable; 66. first round bump; 67. handle; 68. second gear; 69. first round rod; 7. push plate; 71. push rod; 72. T-shaped plate; 73. threaded groove; 74. fifth telescopic rod; 75. threaded rod; 76. first conical tooth; 8. toothed belt; 81. third gear; 82. second conical tooth; 83. second round rod; 84. third conical tooth; 85. fourth conical tooth; 9. slicing knife; 91. T-shaped slide; 92. second round bump; 93. transmission rod. DETAILED DESCRIPTION

[0029] The following is a further detailed description of the embodiments of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0030] See also Figure 1 - Fig. 9The present invention provides a biological tissue paraffin embedding and slicing integrated machine, comprising a base 1, an embedding mechanism for facilitating paraffin embedding of tissues is provided on the base 1, a fixing mechanism for facilitating slicing of wax blocks is provided on the base 1, a cutting mechanism for facilitating slicing of wax blocks is provided on the base 1, the embedding mechanism comprises a transmission frame 21, a metal box 2, a movable rod 3 and a liquid storage tank 13, the fixing mechanism comprises an adjustment frame 4 and an adjustment plate 5, and the cutting mechanism comprises a mounting block 6, a push plate 7 and a slicing knife 9. After the tissue is dipped in wax, the embedding operation of the tissue can be started, the tissue is placed in the metal box 2, a proper amount of wax liquid is dripped into the liquid storage tank 13, after cooling in the cooling box 11, the embedding frame is fixed on the movable plate 55, and the handle 67 is turned to drive the slicing knife 9 to cut reciprocally up and down to obtain slices, so as to facilitate the subsequent drying, staining and sealing of the slices;

[0031] The transmission frame 21 is fixedly mounted on the upper end of the base 1, the upper end of the metal box 2 is fixedly mounted with an L-shaped frame 12, and the liquid storage tank 13 is fixedly mounted on the inner side wall of the L-shaped frame 12. The liquid storage tank 13 is provided with a heating device and a constant temperature device to ensure that the wax liquid is in a liquid state;

[0032] The movable rod 3 is located at the side end of the transmission frame 21, and the adjustment frame 4 is fixedly installed on the side end of the base 1. The side end of the adjustment frame 4 is penetrated by a circular groove 42, and the inner side wall of the circular groove 42 is rotatably installed with a movable shaft 43. There are four adjusting plates 5, and each adjusting plate 5 is fixedly installed on the circumferential end of the movable shaft 43 at an equal distance. The mounting block 6 is fixedly installed on the upper end of the base 1, and a cylinder 61 is fixedly installed on the upper end of the mounting block 6, and a frame 62 is fixedly installed on the upper end of the cylinder 61. Two push rods 71 ​​are fixedly installed on the side end of the push plate 7, and a T-shaped plate 72 is fixedly installed on the side end of the push plate 7. Two fifth telescopic rods 74 are fixedly installed between the side end of the T-shaped plate 72 and the frame 62. When the slicing operation is performed, when the adjusting plate 5 rotates to move to one side of the slicing knife 9 in a vertical state, the user can operate the cylinder 61 to contract, driving the entire frame 62 to move downward, and the downward movement of the frame 62 will drive the slicing knife 9 and the push plate 7 on the frame 62 to move downward at the same time, as shown in the attached figure. Figure 1 As shown, when the push plate 7 moves downward and enters the gap between the movable plate 55 and the bottom plate 52, the push rod 71 at the side end of the push plate 7 will abut against the end of the movable plate 55, and the slicing knife 9 moves downward and approaches the wax block position on the embedding plate at the side end of the fixing frame 51;

[0033] A cooling box 11 is fixedly installed on the upper end of the transmission frame 21. After the metal box 2 enters the cooling box 11, the user can also open the door of the cooling box 11, press the embedding frame on the top of the metal box 2, and then close the door of the cooling box 11, and start the cooling box 11 to cool the wax liquid to solidify the wax liquid. The cooling box 11 is a water cooling box. The water cooling box takes away the heat of the wax liquid through the circulating cold water, so that the wax liquid is quickly cooled. After the wax liquid is cooled and solidified, the embedding plate and the wax block on the embedding plate can be taken out of the metal box 2, and the embedding plate can be fixed in the fixing frame 51 to carry out the next step of slicing.

[0034] A valve 14 is fixedly installed at the lower end of the liquid storage tank 13, a bellows 15 is fixedly installed at the lower end of the valve 14, a valve plate 16 is provided on the inner side wall of the valve 14, a rotating shaft 17 is fixedly installed on the side end of the valve plate 16, a first gear 18 is fixedly installed on the circumferential end of the rotating shaft 17, the rotating shaft 17 penetrates and is rotatably installed on the circumferential end of the valve 14, a sliding groove 19 is penetrated through the side end of the transmission frame 21, a wedge 33 is fixedly installed on the side end of the movable rod 3, the wedge 33 penetrates and is slidably installed on the inner side wall of the sliding groove 19, two first telescopic rods 32 are fixedly installed between the movable rod 3 and the transmission frame 21, two first springs 31 are also fixedly installed between the movable rod 3 and the transmission frame 21, and the two first springs 31 are respectively located on the inner side walls of the two first telescopic rods 32, two connecting members 34 are fixedly installed on the lower end of the two connecting members 34, two cross plates 35 are fixedly installed on the lower ends of the two cross plates 35, and gears are fixedly installed on the upper ends of the two cross plates 35 The rack 36, the rack 36 and the shaft 17 are meshed. When embedding the tissue, the wax-impregnated tissue can be placed in the metal box 2, and the metal box 2 is inserted into the inner wall of the transmission frame 21. After the metal box 2 enters the inner wall of the transmission frame 21, the wedge block 33 will be pushed. The movement of the wedge block 33 will stretch the first telescopic rod 32 and the liquid storage tank 13. At this time, the movement of the wedge block 33 will also drive the movable rod 3 to move. The movement of the movable rod 3 will drive the cross plate 35 to move through the connecting piece 34. The movement of the cross plate 35 will drive the rack 36 to move. The movement of the rack 36 will drive the first gear 18 to rotate. The rotation of the first gear 18 will drive the valve plate 16 to rotate and open in the valve 14 through the shaft 17. At this time, the wax liquid in the liquid storage tank 13 will drip into the metal box 2 through the valve 14 and the bellows 15. The bellows 15 can be retracted. The user can stretch the bellows 15 so that the end of the bellows 15 is as close to the metal box 2 as possible to prevent the wax liquid from splashing to the area outside the metal box 2.

[0035] Four limiting grooves 41 are formed through the side end of the adjustment frame 4, and a pull rod 46 is provided at the side end of the movable shaft 43. A second telescopic rod 45 is fixedly installed between the pull rod 46 and the movable shaft 43, and a second spring 44 is also fixedly installed between the pull rod 46 and the movable shaft 43. The second telescopic rod 45 is located on the inner side wall of the second spring 44, and a limiting rod 47 is fixedly installed on the side end of the pull rod 46. The limiting rod 47 is located on the inner side wall of the limiting groove 41. When fixing the embedded plate, the embedded plate can be placed on the movable plate 55, and the C-shaped positioning plate 53 is pulled close to the movable plate 55 by the third spring 54 to press and fix the embedded plate. Four adjustment plates 5 are provided at the circumferential end of the movable shaft 43. The user can drive the adjustment plate 5 to change its position by rotating the movable shaft 43, so that each adjustment plate 5 can be fixed to the movable plate 55. The section plates 5 can be used alternately, that is, when one of the adjustment plates 5 is at the side end of the slicing knife 9 for slicing, the position of the adjustment plate 5 behind it can be parallel to the transmission frame 21, and the staff can process and fix the next batch of wax blocks that need to be embedded and sliced. When adjusting the adjustment plate 5, the pull rod 46 can be pulled, and the movement of the pull rod 46 will drive the limit rod 47 to move away from the inner side wall of the limit groove 41. At this time, the user can rotate the pull rod 46 to drive the movable shaft 43 to rotate through the second telescopic rod 45. The rotation of the movable shaft 43 can drive each adjustment plate 5 to rotate. After rotating the movable shaft 43 by ninety degrees, the user can release the pull rod 46, and the second spring 44 will pull the pull rod 46 to move and drive the limit rod 47 to insert into the limit groove 41 for reset, and fix the movable shaft 43 again;

[0036] A fixing frame 51 is fixedly installed on the side end of each adjustment plate 5, a bottom plate 52 is fixedly installed on the lower end of each fixing frame 51, a plurality of C-shaped positioning plates 53 are slidably installed on the end of each fixing frame 51, a third spring 54 is fixedly installed between each C-shaped positioning plate 53 and the bottom plate 52, a movable plate 55 is slidably installed on the inner side wall of the fixing frame 51, two fourth springs 56 are fixedly installed between the movable plate 55 and the bottom plate 52, two fourth telescopic rods 57 are fixedly installed between the movable plate 55 and the bottom plate 52, and the two fourth telescopic rods 57 are respectively located on the inner side walls of the fourth spring 56, a side rod 63 is fixedly installed on the side end of the frame 62, a T-shaped slide groove 64 is provided on the side end of the side rod 63, and a turntable 65 is provided on the side end of the frame 62 A handle 67 is fixedly installed on the side end of the turntable 65, and the handle 67 is rotatably installed on the side end of the frame 62. A second gear 68 is fixedly installed on the circumferential end of the handle 67. A first round rod 69 is also fixedly installed on the side end of the frame 62. A threaded groove 73 is penetrated through the side end of the T-shaped plate 72, and a threaded rod 75 is threadedly rotatably installed on the inner side wall of the threaded groove 73. The threaded rod 75 penetrates and is rotatably installed on the side end of the frame 62. A first conical tooth 76 is fixedly installed on the circumferential end of the threaded rod 75, and a third gear 81 is rotatably installed on the circumferential end of the first round rod 69. A toothed belt 8 is sleeved between the third gear 81 and the second gear 68. A second conical tooth 82 is fixedly installed on the side end of the third gear 81. The side end of the frame 62 also penetrates and is rotatably installed A second round rod 83 is installed, and a third conical tooth 84 and a fourth conical tooth 85 are fixedly installed on the circumferential end of the second round rod 83. The third conical tooth 84 and the second conical tooth 82 are meshed with each other, and the fourth conical tooth 85 is meshed with the first conical tooth 76. A T-shaped slide bar 91 is fixedly installed on the side end of the slicing knife 9. The T-shaped slide bar 91 is slidably installed on the inner side wall of the T-shaped slide groove 64. A second round protrusion 92 is fixedly installed on the side end of the T-shaped slide bar 91. A transmission rod 93 is rotatably installed between the second round protrusion 92 and the first round protrusion 66. After completing the adjustment of the cylinder 61, the user can rotate the handle 67. The rotation of the handle 67 drives the turntable 65 to rotate. The rotation of the turntable 65 drives the first round protrusion 66 to rotate. The rotation of the first round protrusion 66 drives the second round protrusion 92 to rotate through the transmission rod 93. The two round protrusions 92 move up and down reciprocatingly, and the movement of the second round protrusion 92 will drive the slicing knife 9 to move up and down reciprocatingly through the T-shaped sliding rod 91 to slice the wax block, and when the handle 67 is rotated, the second gear 68 will be driven to rotate. The rotation of the second gear 68 drives the third gear 81 to rotate through the toothed belt 8, and the rotation of the third gear 81 will drive the second conical tooth 82 to rotate, and the rotation of the second conical tooth 82 will drive the third conical tooth 84 to rotate, and the rotation of the third conical tooth 84 will drive the fourth conical tooth 85 to rotate through the second round rod 83, and the rotation of the fourth conical tooth 85 will drive the threaded rod 75 to rotate through the first conical tooth 76, and the rotation of the threaded rod 75 will drive the T-shaped plate 72 to approach the frame 62 through the thread groove 73, and the movement of the T-shaped plate 72 will drive the push plate 7 to move.The movement of the push plate 7 will drive the push rod 71 to move, and the movement of the push rod 71 will gradually push the movable plate 55 to move. The movement of the movable plate 55 will stretch the fourth spring 56 and the third spring 54. The movement of the movable plate 55 will drive the fixed embedding plate and wax block on the movable plate 55 to move at a uniform speed, so that the slicing knife 9 can complete the slicing work of the next time after the slicing is completed, and the slicing is uniform.

[0037] Working principle:

[0038] The first step is paraffin embedding and sectioning, which is a commonly used histological technique for preparing biological tissue samples for observation and analysis under a microscope. Fresh biological tissue samples are placed in an appropriate fixative, such as formalin, to prevent tissue autolysis and corruption. The fixed tissue needs to be dehydrated through a series of ethanol solutions with increasing concentrations to remove moisture from the tissue, usually starting from a low concentration of ethanol and gradually increasing to a high concentration of ethanol. Each concentration is soaked for a certain period of time. The dehydrated tissue is placed in a transparent agent, such as xylene, to make the tissue transparent so that paraffin can penetrate better. The transparent time is generally tens of minutes. The transparent tissue is placed in molten paraffin and soaked at an appropriate temperature for a period of time so that the paraffin fully penetrates into the tissue. After the tissue is waxed, the embedding operation of the tissue can be started. The tissue is placed in a metal box 2, and an appropriate amount of wax liquid is dripped through a liquid storage tank 13. After cooling in a cooling box 11, the embedding frame is fixed on a movable plate 55, and the handle 67 is turned to drive the slicer 9 to cut up and down to obtain slices, so as to facilitate subsequent drying, staining and sealing of the slices.

[0039] In the second step, when embedding the tissue, the wax-impregnated tissue can be placed in the metal box 2, and the metal box 2 is inserted into the inner wall of the transmission frame 21. After the metal box 2 enters the inner wall of the transmission frame 21, the wedge block 33 will be pushed. The movement of the wedge block 33 will stretch the first telescopic rod 32 and the liquid storage tank 13. At this time, the movement of the wedge block 33 will also drive the movable rod 3 to move. The movement of the movable rod 3 will drive the cross plate 35 to move through the connecting piece 34. The movement of the cross plate 35 will drive the rack 36 to move. The movement of the rack 36 will drive the first gear 18 to rotate. The rotation of the first gear 18 will drive the valve plate 16 to rotate and open in the valve 14 through the rotating shaft 17. At this time, the wax liquid in the liquid storage tank 13 The wax will drip into the metal box 2 through the valve 14 and the bellows 15. The bellows 15 can be extended and retracted. The user can stretch the bellows 15 so that the end of the bellows 15 is as close to the metal box 2 as possible to prevent the wax liquid from splashing into the area outside the metal box 2 and affecting the working environment. When an appropriate amount of wax liquid drips into the metal box 2, the user can push the metal box 2 into the inner wall of the cooling box 11. When the metal box 2 leaves the end of the wedge block 33, the first telescopic rod 32 will rebound and drive the movable rod 3 to move and reset. The rack 36 on the movable rod 3 drives the first gear 18 to rotate, and then drives the valve plate 16 to rotate to close the valve 14, so that the wax liquid no longer drips.

[0040] The device is provided with a metal box 2, a movable rod 3 and a valve plate 16, which cooperate with each other. When the metal box 2 moves to the bottom of the bellows 15, the valve plate 16 can be driven to open automatically. When the metal box 2 leaves the bottom of the bellows 15, the valve plate 16 can be automatically driven to close. There is no need to manually operate the valve plate 16, which reduces the time and steps of manual intervention. It can ensure that the wax liquid will drip only when the metal box 2 is in the correct position each time. In this way, the amount of wax liquid can be accurately controlled to avoid affecting the embedding quality due to too much or too little wax liquid. When the metal box 2 leaves the liquid outlet, the valve 14 is automatically closed, which can effectively prevent the accidental dripping of wax liquid and reduce the pollution to the working environment.

[0041] The device is provided with a valve plate 16 that opens and closes automatically. The opening and closing of the valve plate 16 can be changed by changing the position of the metal box 2, so that there is no need to manually adjust the valve plate 16. Because the wax liquid usually remains in a liquid state at a higher temperature, the manual operation of the valve plate 16 is easy to contact the high-temperature wax liquid, which poses a risk of scalding. The cooperation of the movable rod 3, the rack 36 and the first gear 18 enables the opening and closing of the valve plate 16 to be achieved without manual contact or proximity to the valve 14 area, so that the operator can stay away from the high-temperature area to avoid the occurrence of scalding accidents.

[0042] In the third step, after the metal box 2 enters the cooling box 11, the user can also open the door of the cooling box 11, press the embedding frame on the top of the metal box 2, and then close the door of the cooling box 11, and start the cooling box 11 to cool the wax liquid to solidify the wax liquid. The cooling box 11 is a water cooling box. The water cooling box takes away the heat of the wax liquid through the circulating cold water, so that the wax liquid is quickly cooled. After the wax liquid is cooled and solidified, the embedding plate and the wax block on the embedding plate can be taken out of the metal box 2, and the embedding plate is fixed in the fixing frame 51 to carry out the next step of slicing.

[0043] In the fourth step, when fixing the embedding plate, the embedding plate can be placed on the movable plate 55, and the C-shaped positioning plate 53 is pulled close to the movable plate 55 by the third spring 54 to press and fix the embedding plate. Four adjustment plates 5 are arranged at the circumferential end of the movable shaft 43. The user can drive the adjustment plate 5 to change its position by rotating the movable shaft 43, so that each adjustment plate 5 can be used alternately, that is, when one of the adjustment plates 5 is at the end of the slicing knife 9 for slicing, the position of the adjustment plate 5 behind it can be parallel to the transmission frame 21, and the staff can adjust the position of the next batch of slices to be cut. To process and fix the wax block for embedding and sectioning, when adjusting the adjustment plate 5, the pull rod 46 can be pulled, and the movement of the pull rod 46 will drive the limit rod 47 to move away from the inner wall of the limit groove 41. At this time, the user can rotate the pull rod 46 to drive the movable shaft 43 to rotate through the second telescopic rod 45. The rotation of the movable shaft 43 can drive each adjustment plate 5 to rotate. After rotating the movable shaft 43 by ninety degrees, the user can release the pull rod 46, and the second spring 44 will pull the pull rod 46 to move and drive the limit rod 47 to insert into the limit groove 41 to reset, and fix the movable shaft 43 again;

[0044] The device realizes the parallel operation of the processes of loading, slicing, and disassembling by arranging the adjustment plate 5 and the fixing frame 51 which can work alternately, thus avoiding the time waste of having to wait for one process to be completed before proceeding to the next process in the existing method. Multiple workbenches work alternately, which greatly shortens the overall operation time and improves the slicing efficiency for laboratories or production environments that require a large amount of slicing and loading.

[0045] The device is provided with an adjustment plate 5, a fixed frame 51 and a movable shaft 43 that are used alternately. The alternating work tables allow operators to clearly divide the work. One part of the operators focuses on the slicing operation, while the other part is responsible for the loading work. The clear division of labor can also reduce the switching time and error rate of the operators between different processes, improve the coordination and fluency of the overall work, and enable the manpower in the laboratory to be flexibly allocated to optimize the work efficiency.

[0046] In the fifth step, when the slicing operation is performed, when the adjustment plate 5 rotates to a vertical position and moves to one side of the slicing knife 9, the user can operate the cylinder 61 to contract, driving the entire frame 62 to move downward. The downward movement of the frame 62 will drive the slicing knife 9 and the push plate 7 on the frame 62 to move downward at the same time, as shown in the attached figure. Figure 1 As shown, when the push plate 7 moves downward and enters the gap between the movable plate 55 and the bottom plate 52, the push rod 71 at the side end of the push plate 7 will abut against the end of the movable plate 55, and the slicing knife 9 will move downward and approach the wax block position on the embedding plate at the side end of the fixed frame 51. After completing the adjustment of the cylinder 61, the user can turn the handle 67. The rotation of the handle 67 drives the turntable 65 to rotate. The rotation of the turntable 65 drives the first round protrusion 66 to rotate. The rotation of the first round protrusion 66 drives the second round protrusion 92 to move up and down reciprocatingly through the transmission rod 93. The movement of the second round protrusion 92 drives the slicing knife 9 to move up and down reciprocatingly through the T-shaped slide bar 91 to slice the wax block. When the handle 67 rotates, it also drives the second gear 68 to rotate. The second gear 68 rotates and drives the third gear 81 to rotate through the toothed belt 8. The rotation of the third gear 81 will drive The second conical tooth 82 is driven to rotate, and the rotation of the second conical tooth 82 drives the third conical tooth 84 to rotate. The rotation of the third conical tooth 84 drives the fourth conical tooth 85 to rotate through the second round rod 83. The rotation of the fourth conical tooth 85 drives the threaded rod 75 to rotate through the first conical tooth 76. The rotation of the threaded rod 75 drives the T-shaped plate 72 to approach the frame 62 through the thread groove 73. The movement of the T-shaped plate 72 drives the push plate 7 to move. The movement of the push plate 7 drives the push rod 71 to move. The movement of the push rod 71 gradually drives the movable plate 55 to move. The movement of the movable plate 55 will stretch the fourth spring 56 and the third spring 54. The movement of the movable plate 55 will drive the fixed embedding plate and the wax block on the movable plate 55 to move at a uniform speed, so that after the slicing knife 9 reciprocates up and down for slicing, the next slicing work can be carried out after one slicing is completed, and the slicing is uniform.

[0047] The device is provided with an embedding mechanism and a slicing mechanism in cooperation, so that the embedding mechanism and the slicing mechanism are integrated. The integrated design allows the tissue sample to be directly sliced ​​after embedding. Since the sample does not need to be moved between different devices, the risk of sample damage caused by transportation, collision, etc. is reduced, the intermediate operation steps and time waste are reduced, and the space utilization rate of the experimental environment is improved, ensuring work efficiency.

[0048] The device is provided with a threaded rod 75, a T-shaped plate 72, a push plate 7 and a movable plate 55, which cooperate with each other. When the handle 67 is turned to drive the slicer 9 to move back and forth for cutting, the wax block on the embedding plate is driven to move at a uniform speed, which can ensure that the speed of the wax block passing through the blade is constant during each cutting, so that the thickness of the slices cut is more uniform, avoiding the problem of excessive difference in slice thickness caused by unstable movement speed of the wax block, ensuring the slice efficiency while ensuring the quality of the slice, and having better stability.

[0049] The embodiments of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

Claims

1. A biological tissue paraffin embedding and slicing machine, comprising a base (1), characterized in that: The base (1) is provided with an embedding mechanism for facilitating paraffin embedding of tissues, the base (1) is provided with a fixing mechanism for facilitating slicing of wax blocks, and the base (1) is provided with a cutting mechanism for facilitating slicing of wax blocks; The embedding mechanism comprises a transmission frame (21), a metal box (2), a movable rod (3) and a liquid storage tank (13); the fixing mechanism comprises an adjustment frame (4) and an adjustment plate (5); the cutting mechanism comprises a mounting block (6), a push plate (7) and a slicing knife (9); the transmission frame (21) is fixedly mounted on the upper end of the base (1); an L-shaped stand (12) is fixedly mounted on the upper end of the metal box (2); the liquid storage tank (13) is fixedly mounted on the inner side wall of the L-shaped stand (12); the movable rod (3) is located at the side end of the transmission frame (21); the adjustment frame (4) is fixedly mounted on the side end of the base (1); and a circular groove ( 42), a movable shaft (43) is rotatably mounted on the inner side wall of the circular groove (42), the number of the adjusting plates (5) is four, each of the adjusting plates (5) is fixedly mounted on the circumferential end of the movable shaft (43) at equal distances, the mounting block (6) is fixedly mounted on the upper end of the base (1), a cylinder (61) is fixedly mounted on the upper end of the mounting block (6), a frame (62) is fixedly mounted on the upper end of the cylinder (61), two push rods (71) are fixedly mounted on the side end of the push plate (7), a T-shaped plate (72) is fixedly mounted on the side end of the push plate (7), and two fifth telescopic rods (74) are fixedly mounted between the side end of the T-shaped plate (72) and the frame (62).

2. A biological tissue paraffin embedding and slicing integrated machine as claimed in claim 1, characterized in that: A cooling box (11) is fixedly mounted on the upper end of the transmission frame (21), a valve (14) is fixedly mounted on the lower end of the liquid storage box (13), a bellows (15) is fixedly mounted on the lower end of the valve (14), and a valve plate (16) is provided on the inner side wall of the valve (14); Wherein, a rotating shaft (17) is fixedly mounted on the side end of the valve plate (16), and a first gear (18) is fixedly mounted on the circumferential end of the rotating shaft (17); The rotating shaft (17) is rotatably mounted on the circumferential end of the valve (14).

3. A biological tissue paraffin embedding and slicing integrated machine as claimed in claim 2, characterized in that: A slide groove (19) is penetrated through the side end of the transmission frame (21), a wedge block (33) is fixedly installed on the side end of the movable rod (3), and the wedge block (33) is slidably installed on the inner side wall of the slide groove (19), two first telescopic rods (32) are fixedly installed between the movable rod (3) and the transmission frame (21), and two first springs (31) are also fixedly installed between the movable rod (3) and the transmission frame (21); Wherein, the two first springs (31) are respectively located on the inner side walls of the two first telescopic rods (32).

4. A biological tissue paraffin embedding and slicing integrated machine as claimed in claim 3, characterized in that: Two connecting members (34) are fixedly mounted on the lower end of the movable rod (3), two transverse plates (35) are fixedly mounted on the lower ends of the two connecting members (34), and racks (36) are fixedly mounted on the upper ends of the two transverse plates (35), and the racks (36) are meshed with the rotating shaft (17).

5. A biological tissue paraffin embedding and slicing integrated machine as claimed in claim 4, characterized in that: Four limiting grooves (41) are formed through the side end of the adjustment frame (4), a pull rod (46) is formed at the side end of the movable shaft (43), a second telescopic rod (45) is fixedly installed between the pull rod (46) and the movable shaft (43), a second spring (44) is also fixedly installed between the pull rod (46) and the movable shaft (43), and the second telescopic rod (45) is located on the inner side wall of the second spring (44); Wherein, a limiting rod (47) is fixedly installed on the side end of the pull rod (46), and the limiting rod (47) is located on the inner side wall of the limiting groove (41).

6. A biological tissue paraffin embedding and slicing integrated machine as claimed in claim 5, characterized in that: A fixing frame (51) is fixedly mounted on the side end of each adjustment plate (5), a bottom plate (52) is fixedly mounted on the lower end of each fixing frame (51), a plurality of C-shaped positioning plates (53) are slidably mounted on the end of each fixing frame (51), and a third spring (54) is fixedly mounted between each C-shaped positioning plate (53) and the bottom plate (52).

7. A biological tissue paraffin embedding and slicing integrated machine as claimed in claim 6, characterized in that: A movable plate (55) is also slidably mounted on the inner side wall of the fixed frame (51), two fourth springs (56) are fixedly mounted between the movable plate (55) and the bottom plate (52), and two fourth telescopic rods (57) are also fixedly mounted between the movable plate (55) and the bottom plate (52); Wherein, the two fourth telescopic rods (57) are respectively located on the inner side walls of the fourth spring (56).

8. The biological tissue paraffin embedding and slicing integrated machine as claimed in claim 7, characterized in that: A side rod (63) is fixedly mounted on the side end of the frame (62), a T-shaped slide groove (64) is provided on the side end of the side rod (63), a turntable (65) is provided on the side end of the frame (62), a handle (67) is fixedly mounted on the side end of the turntable (65), the handle (67) is rotatably mounted on the side end of the frame (62), a second gear (68) is fixedly mounted on the circumferential end of the handle (67), and a first round rod (69) is also fixedly mounted on the side end of the frame (62).

9. The biological tissue paraffin embedding and slicing integrated machine as claimed in claim 8, characterized in that: A thread groove (73) is formed through the side end of the T-shaped plate (72); a threaded rod (75) is threadedly rotatably mounted on the inner side wall of the thread groove (73); the threaded rod (75) is rotatably mounted on the side end of the frame (62); a first conical tooth (76) is fixedly mounted on the circumferential end of the threaded rod (75); a third gear (81) is rotatably mounted on the circumferential end of the first round rod (69); a toothed belt (8) is sleeved between the third gear (81) and the second gear (68); and a second conical tooth (82) is fixedly mounted on the side end of the third gear (81); The side end of the frame (62) is also penetrated by a second round rod (83) rotatably installed, and the circumferential end of the second round rod (83) is fixedly installed with a third conical tooth (84) and a fourth conical tooth (85), the third conical tooth (84) is meshed with the second conical tooth (82), and the fourth conical tooth (85) is meshed with the first conical tooth (76).

10. The biological tissue paraffin embedding and slicing integrated machine as claimed in claim 9, characterized in that: A T-shaped slide bar (91) is fixedly mounted on the side end of the slicing knife (9), and the T-shaped slide bar (91) is slidably mounted on the inner side wall of the T-shaped slide groove (64). A second round protrusion (92) is fixedly mounted on the side end of the T-shaped slide bar (91), and a transmission rod (93) is rotatably mounted between the second round protrusion (92) and the first round protrusion (66).

Images