Atomized liquid dressing composition for repairing cell microvesicles and preparation method thereof

Abstract

The invention discloses an atomized liquid dressing composition for repairing cell microvesicles and a preparation method of the atomized liquid dressing composition. Comprising the following raw materials: 1.0%-8.0% of a sugar source, 0.01%-10.0% of algal polysaccharides, 0.01%-5.0% of sodium alginate, 0.01%-2% of sodium bicarbonate, 0.001%-0.08% of hydroxyethyl cellulose, 0.1%-2% of hyaluronic acid, 0.5%-2% of carboxymethyl chitosan, 0.1%-0.9% of sodium chloride, 0.03%-0.6% of a humectant, 0.05%-0.5% of an osmotic pressure regulator and the balance of distilled water or purified water. By means of sodium alginate, hydroxyethyl cellulose and the sugar source, the space structure of mucus can be changed, the mucoprotein concentration, cohesiveness and elasticity can be reduced, the pH value is adjusted in combination with sodium bicarbonate, decomposition and mutation of the sugar source are avoided, and then the storage stability of the atomized liquid dressing is improved.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to an atomized liquid dressing composition for repairing cell microvesicles and a preparation method thereof. Background Art

[0002] Cell microvesicles, also known as exosomes, are membrane-secreted vesicle-like structures with a diameter of 30 to 150 nm. Their topological structure is similar to that of cells and are rich in specific proteins, lipids, nucleic acids and polysaccharide complexes.

[0003] In medicine, dressings generally refer to temporary protective coverings that are placed on wounds. When the skin is injured, choosing a suitable dressing to cover the wound is helpful in controlling bleeding, preventing infection, and absorbing secretions to promote rapid wound healing.

[0004] However, there are few medical dressing products for wound repair, especially for chronic wounds, which have slow healing speed and long course of disease. At the same time, the storage stability of aerosolized liquid dressings is poor. Therefore, we propose an aerosolized liquid dressing composition for cell microvesicle repair and its preparation method to solve the above-mentioned problems. Summary of the invention

[0005] The purpose of the present invention is to provide an atomized liquid dressing composition for cell microvesicle repair and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The atomized liquid dressing composition for cell microvesicle repair includes the following raw materials by weight percentage: 1.0%-8.0% of sugar source, 0.01%-10.0% of seaweed polysaccharide, 0.01%-5.0% of sodium alginate, 0.01%-2% of sodium bicarbonate, 0.001%-0.08% of hydroxyethyl cellulose, 0.1%-2% of hyaluronic acid, 0.5%-2% of carboxymethyl chitosan, 0.1%-0.9% of sodium chloride, 0.03%-0.6% of moisturizer, 0.05%-0.5% of osmotic pressure regulator, and the rest is distilled water or purified water.

[0008] As a further solution of the present invention: the raw materials include the following components in weight percentage: 0.8%-4.0% sugar source, 0.05%-5.0% seaweed polysaccharide, 0.03%-4.0% sodium alginate, 0.03%-3% sodium bicarbonate, 0.004%-0.12% hydroxyethyl cellulose, 0.2%-4% hyaluronic acid, 0.6%-3% carboxymethyl chitosan, 0.5%-1.2% sodium chloride, 0.01%-0.8% moisturizer, 0.04%-0.8% osmotic pressure regulator, and the rest is distilled water or purified water.

[0009] As a further solution of the present invention: the raw materials include the following components in weight percentage: 2.0%-3.0% sugar source, 0.06%-3.0% seaweed polysaccharide, 0.04%-3.0% sodium alginate, 0.04%-1% sodium bicarbonate, 0.005%-0.13% hydroxyethyl cellulose, 0.3%-5% hyaluronic acid, 0.4%-2.3% carboxymethyl chitosan, 0.3%-0.8% sodium chloride, 0.04%-0.9% moisturizer, 0.03%-0.5% osmotic pressure regulator, and the rest is distilled water or purified water.

[0010] As a further solution of the present invention: the raw materials include the following components in weight percentage: 1.4%-9.0% of sugar source, 0.02%-2.0% of seaweed polysaccharide, 0.05%-6.0% of sodium alginate, 0.05%-3% of sodium bicarbonate, 0.004%-0.14% of hydroxyethyl cellulose, 0.2%-2.3% of hyaluronic acid, 0.6%-2.5% of carboxymethyl chitosan, 0.23%-1.5% of sodium chloride, 0.08%-0.7% of moisturizing agent, 0.04%-0.7% of osmotic pressure regulator, and the rest is distilled water or purified water.

[0011] As a further solution of the present invention: the raw materials include the following components in weight percentage: 1.6%-13.0% of sugar source, 0.03%-2.0% of seaweed polysaccharide, 0.03%-1.0% of sodium alginate, 0.07%-2.4% of sodium bicarbonate, 0.009%-0.14% of hydroxyethyl cellulose, 0.15%-2.2% of hyaluronic acid, 0.51%-3.2% of carboxymethyl chitosan, 0.15%-1.3% of sodium chloride, 0.12%-0.8% of moisturizing agent, 0.02%-0.55% of osmotic pressure regulator, and the rest is distilled water or purified water.

[0012] As a further solution of the present invention: the raw materials include the following components in weight percentage: 1.3%-8.5% of sugar source, 0.03%-10.5% of seaweed polysaccharide, 0.02%-5.5% of sodium alginate, 0.023%-2.5% of sodium bicarbonate, 0.007%-0.15% of hydroxyethyl cellulose, 0.15%-2.5% of hyaluronic acid, 0.45%-2.15% of carboxymethyl chitosan, 0.15%-0.95% of sodium chloride, 0.035%-0.65% of moisturizing agent, 0.15%-0.25% of osmotic pressure regulator, and the rest is distilled water or purified water.

[0013] As a further solution of the present invention: the seaweed polysaccharide includes guluronic acid oligosaccharide, and the mass percentage of the guluronic acid oligosaccharide in the seaweed polysaccharide is between 70% and 88%.

[0014] As a further embodiment of the present invention: the seaweed polysaccharide further comprises one or more of carrageenan oligosaccharide, agar oligosaccharide, seaweed cellulose, xylan, mannan and agar.

[0015] As a further embodiment of the present invention: the sugar source includes one or more of brown algae polysaccharides and G-type brown algae oligosaccharides.

[0016] A method for preparing a nebulized liquid dressing composition for cell microvesicle repair comprises the following steps:

[0017] Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution;

[0018] Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution;

[0019] Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution;

[0020] Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The cell microcapsule repair atomized liquid dressing composition and preparation method thereof can change the spatial structure of mucus, reduce the concentration, cohesion and elasticity of mucin through sodium alginate, hydroxyethyl cellulose and sugar source, and adjust the pH value in combination with sodium bicarbonate to avoid the decomposition and mutation of the sugar source, thereby improving the storage stability of the atomized liquid dressing; the preparation method is simple and feasible, provides a moist healing environment for the wound through the synergistic effect of multiple components, promotes collagen production, shortens the wound healing time, and effectively promotes wound healing, has broad development and application prospects, and the preparation process is simple, green and highly safe, which is conducive to industrial scale-up production. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The present invention is a schematic flow chart of the method for preparing the atomized liquid dressing composition for cell microvesicle repair. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The present invention provides a technical solution: The technical solution of this patent is further described in detail below in conjunction with a specific implementation method.

[0026] Example 1

[0027] The atomized liquid dressing composition for cell microvesicle repair comprises the following raw materials by weight percentage: 1.0% sugar source, 0.01% seaweed polysaccharide, 0.01% sodium alginate, 0.01% sodium bicarbonate, 0.001% hydroxyethyl cellulose, 0.1% hyaluronic acid, 0.5% carboxymethyl chitosan, 0.1% sodium chloride, 0.03% moisturizer, 0.05% osmotic pressure regulator, and the rest is distilled water or purified water;

[0028] The preparation method of the atomized liquid dressing composition for cell microvesicle repair comprises the following steps:

[0029] Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution;

[0030] Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution;

[0031] Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution;

[0032] Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

[0033] Example 2

[0034] The atomized liquid dressing composition for cell microvesicle repair comprises the following raw materials by weight percentage: 8.0% of sugar source, 10.0% of seaweed polysaccharide, 5.0% of sodium alginate, 0.01% of sodium bicarbonate, 0.001% of hydroxyethyl cellulose, 0.1% of hyaluronic acid, 0.5% of carboxymethyl chitosan, 0.1% of sodium chloride, 0.6% of moisturizer, 0.05% of osmotic pressure regulator, and the rest is distilled water or purified water;

[0035] The preparation method of the atomized liquid dressing composition for cell microvesicle repair comprises the following steps:

[0036] Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution;

[0037] Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution;

[0038] Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution;

[0039] Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

[0040] Example 3

[0041] The atomized liquid dressing composition for cell microvesicle repair comprises the following raw materials by weight percentage: 8.0% of sugar source, 10.0% of seaweed polysaccharide, 0.01% of sodium alginate, 0.01% of sodium bicarbonate, 0.08% of hydroxyethyl cellulose, 0.1% of hyaluronic acid, 0.5% of carboxymethyl chitosan, 0.1% of sodium chloride, 0.03% of moisturizer, 0.05% of osmotic pressure regulator, and the rest is distilled water or purified water;

[0042] The preparation method of the atomized liquid dressing composition for cell microvesicle repair comprises the following steps:

[0043] Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution;

[0044] Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution;

[0045] Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution;

[0046] Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

[0047] Example 4

[0048] The atomized liquid dressing composition for cell microvesicle repair comprises the following raw materials by weight percentage: 1.0% sugar source, 0.01% seaweed polysaccharide, 5.0% sodium alginate, 2% sodium bicarbonate, 0.08% hydroxyethyl cellulose, 0.1% hyaluronic acid, 0.5% carboxymethyl chitosan, 0.1% sodium chloride, 0.03% moisturizer, 0.5% osmotic pressure regulator, and the rest is distilled water or purified water;

[0049] The preparation method of the atomized liquid dressing composition for cell microvesicle repair comprises the following steps:

[0050] Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution;

[0051] Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution;

[0052] Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution;

[0053] Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

[0054] Example 5

[0055] The atomized liquid dressing composition for cell microvesicle repair comprises the following raw materials by weight percentage: 8.0% sugar source, 0.01% seaweed polysaccharide, 0.01% sodium alginate, 2% sodium bicarbonate, 0.001% hydroxyethyl cellulose, 0.1% hyaluronic acid, 0.5% carboxymethyl chitosan, 0.9% sodium chloride, 0.6% moisturizer, 0.5% osmotic pressure regulator, and the rest is distilled water or purified water;

[0056] The preparation method of the atomized liquid dressing composition for cell microvesicle repair comprises the following steps:

[0057] Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution;

[0058] Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution;

[0059] Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution;

[0060] Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

[0061] Clinical efficacy trial

[0062] By comparing the clinical efficacy of the atomized liquid dressings prepared in Examples 1-5 with conventional treatments, the therapeutic effects and advantages of the atomized liquid dressings can be obtained;

[0063] Case selection: 40 patients with clinical records were randomly selected and divided into two groups: a conventional treatment group and an experimental group (Example 1, Example 2, Example 3, Example 4 and Example 5).

[0064] Treatment method: The conventional treatment group used conventional treatment, and the specific usage and dosage were in accordance with the clinical medication standards, and the observation period was 1 week; the experimental group used the atomized liquid dressing prepared in Example 1, Example 2, Example 3, Example 4 and Example 5 on the basis of conventional treatment, sprayed on the corresponding parts, 3 times a day, and the observation period was 1 week;

[0065] Scoring criteria: 0 points for deterioration, 1 point for no effect, 2-3 points for slight improvement, 4-6 points for moderate improvement, and 7-9 points for marked improvement.

[0066] Statistical methods: Statistical software was used to analyze the data, and the effect was scored at the end of treatment. A repeated measures variance design was used for analysis. The scores are shown in Table 1.

[0067] Table 1 Comparison of symptom scores before and after treatment

[0068] Group Fraction Conventional treatment group 3.45±1.15 Example 1 4.67±1.34 Example 2 5.45±1.76 Example 3 5.25±1.35 Example 4 6.15±1.05 Example 5 6.35±1.12

[0069] As can be seen from Table 1, the atomized liquid dressings prepared by Example 1, Example 2, Example 3, Example 4 and Example 5 have a certain therapeutic effect on the repair and oil collection of cell microvesicles; sodium alginate, hydroxyethyl cellulose and sugar source can change the spatial structure of mucus, reduce the concentration, cohesion and elasticity of mucin, and adjust the pH value with sodium bicarbonate to avoid the decomposition and mutation of the sugar source, thereby improving the storage stability of the atomized liquid dressing; the preparation method is simple and feasible, and provides a moist healing environment for the wound through the synergistic effect of multiple components, promotes collagen production, shortens the wound healing time, and effectively promotes wound healing. It has broad development and application prospects, and the preparation process is simple, green, and highly safe, which is conducive to industrial scale-up production.

[0070] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. Atomized liquid dressing composition for cell microvesicle repair, characterized in that: The raw materials include the following components in percentage by weight: 1.0%-8.0% of sugar source, 0.01%-10.0% of seaweed polysaccharide, 0.01%-5.0% of sodium alginate, 0.01%-2% of sodium bicarbonate, 0.001%-0.08% of hydroxyethyl cellulose, 0.1%-2% of hyaluronic acid, 0.5%-2% of carboxymethyl chitosan, 0.1%-0.9% of sodium chloride, 0.03%-0.6% of moisturizing agent, 0.05%-0.5% of osmotic pressure regulator, and the rest is distilled water or purified water.

2. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The raw materials include the following components in percentage by weight: 0.8%-4.0% of sugar source, 0.05%-5.0% of seaweed polysaccharide, 0.03%-4.0% of sodium alginate, 0.03%-3% of sodium bicarbonate, 0.004%-0.12% of hydroxyethyl cellulose, 0.2%-4% of hyaluronic acid, 0.6%-3% of carboxymethyl chitosan, 0.5%-1.2% of sodium chloride, 0.01%-0.8% of moisturizing agent, 0.04%-0.8% of osmotic pressure regulator, and the rest is distilled water or purified water.

3. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The raw materials include the following components in percentage by weight: 2.0%-3.0% of sugar source, 0.06%-3.0% of seaweed polysaccharide, 0.04%-3.0% of sodium alginate, 0.04%-1% of sodium bicarbonate, 0.005%-0.13% of hydroxyethyl cellulose, 0.3%-5% of hyaluronic acid, 0.4%-2.3% of carboxymethyl chitosan, 0.3%-0.8% of sodium chloride, 0.04%-0.9% of moisturizing agent, 0.03%-0.5% of osmotic pressure regulator, and the rest is distilled water or purified water.

4. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The raw materials include the following components in percentage by weight: 1.4%-9.0% of sugar source, 0.02%-2.0% of seaweed polysaccharide, 0.05%-6.0% of sodium alginate, 0.05%-3% of sodium bicarbonate, 0.004%-0.14% of hydroxyethyl cellulose, 0.2%-2.3% of hyaluronic acid, 0.6%-2.5% of carboxymethyl chitosan, 0.23%-1.5% of sodium chloride, 0.08%-0.7% of moisturizing agent, 0.04%-0.7% of osmotic pressure regulator, and the rest is distilled water or purified water.

5. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The raw materials include the following components in percentage by weight: 1.6%-13.0% of sugar source, 0.03%-2.0% of seaweed polysaccharide, 0.03%-1.0% of sodium alginate, 0.07%-2.4% of sodium bicarbonate, 0.009%-0.14% of hydroxyethyl cellulose, 0.15%-2.2% of hyaluronic acid, 0.51%-3.2% of carboxymethyl chitosan, 0.15%-1.3% of sodium chloride, 0.12%-0.8% of moisturizing agent, 0.02%-0.55% of osmotic pressure regulator, and the rest is distilled water or purified water.

6. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The raw materials include the following components in percentage by weight: 1.3%-8.5% of sugar source, 0.03%-10.5% of seaweed polysaccharide, 0.02%-5.5% of sodium alginate, 0.023%-2.5% of sodium bicarbonate, 0.007%-0.15% of hydroxyethyl cellulose, 0.15%-2.5% of hyaluronic acid, 0.45%-2.15% of carboxymethyl chitosan, 0.15%-0.95% of sodium chloride, 0.035%-0.65% of moisturizing agent, 0.15%-0.25% of osmotic pressure regulator, and the rest is distilled water or purified water.

7. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The seaweed polysaccharide comprises guluronic acid oligosaccharide, and the mass percentage of the guluronic acid oligosaccharide in the seaweed polysaccharide is between 70% and 88%.

8. The atomized liquid dressing composition for cell microvesicle repair according to claim 7, characterized in that: The seaweed polysaccharide also includes one or more of carrageenan oligosaccharide, agar oligosaccharide, seaweed cellulose, xylan, mannan, and agar.

9. The atomized liquid dressing composition for cell microvesicle repair according to claim 1, characterized in that: The sugar source includes one or more of brown algae polysaccharide and G-type brown algae oligosaccharide.

10. A method for preparing a nebulized liquid dressing composition for cell microvesicle repair, characterized in that: The atomized liquid dressing composition for cell microvesicle repair according to any one of claims 1 to 9, wherein the preparation method comprises the following steps: Step 1: Add a predetermined weight of sodium alginate and sodium chloride into a sufficient amount of distilled water or purified water and stir to obtain a sodium alginate-sodium chloride solution; Step 2: adding a predetermined weight of sugar source, seaweed polysaccharide, sodium bicarbonate, and hydroxyethyl cellulose to the sodium alginate-sodium chloride solution, stirring, and filtering to obtain a first mixed solution; Step 3: adding a predetermined weight of hyaluronic acid, carboxymethyl chitosan, moisturizer, and osmotic pressure regulator into a sufficient amount of distilled water or purified water and stirring to obtain a second mixed solution; Step 4: Evenly mix the first mixed solution and the second mixed solution to obtain an atomized liquid dressing.

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