Plant extract for inhibiting helicobacter pylori as well as preparation method and application of plant extract

Abstract

The invention discloses a plant extract for inhibiting helicobacter pylori as well as a preparation method and application of the plant extract. The plant extract comprises dandelion, fennel and pomegranate bark. According to the invention, dandelion, fennel and pomegranate rind are adopted as active ingredients, and the traditional Chinese medicine composition can be used for preparing medicines for preventing / treating helicobacter pylori. The invention develops a new field for the application of traditional Chinese medicinal materials, and provides a new direction for the research and development of high-efficiency low-toxicity helicobacter pylori-resistant medicines or the development of health-care foods.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine, and in particular to a plant extract for inhibiting Helicobacter pylori, comprising dandelion, pomegranate peel and fennel, and application of the plant extract in preparing a medicine for preventing / treating Helicobacter pylori. Background Art

[0002] Helicobacter pylori (HP) is a microaerophilic Gram-negative bacterium commonly found in the human gastric mucosa. HP infection is an important cause of gastrointestinal diseases such as gastric ulcer, duodenal ulcer, and gastric cancer. Currently, most clinical treatment regimens are based on proton pump inhibitors (PPIs) and bismuth agents, combined with quadruple therapy consisting of two antibiotics. Although the eradication rate is relatively high, these drugs currently have problems such as large toxic side effects, easy secondary drug resistance, superinfection, and patient compliance. Drug-resistant strains are also the main reason for the decline in the success rate of drug eradication of Helicobacter pylori (HP). Therefore, it is necessary to seek ideal drugs with fewer side effects, no drug resistance, and low recurrence rate. Summary of the invention

[0003] Based on the above reasons, in view of the problems or defects in the prior art, the present invention aims to provide a plant extract for inhibiting Helicobacter pylori and its preparation method and application. The plant extract of the present invention has a milder efficacy, avoids large toxic side effects, and can effectively inhibit bacteria.

[0004] The technical solution adopted by the present invention is as follows:

[0005] A plant extract comprising dandelion, fennel and pomegranate peel.

[0006] A plant extract comprises 30-40 parts by weight of dandelion, 22-25 parts by weight of fennel and 10-15 parts by weight of pomegranate peel.

[0007] The second object of the present invention is to provide a method for preparing a plant extract, comprising the following steps:

[0008] S1. Take dried pomegranate peel, fennel and dandelion and crush them into powder using a grinding device;

[0009] S2, extracting by reflux, filtering, extracting by reflux again the residue, and drying by freeze drying to obtain powder, thereby obtaining the plant extract.

[0010] Preferably, the extraction is performed by reflux with water, ethanol or ethanol-water solution.

[0011] Alternatively, the present invention provides another method for preparing a plant extract, comprising the following steps:

[0012] Take pomegranate peel, fennel and dandelion respectively, add appropriate amount of water to soak for 30 minutes, then decoct for 30 minutes, filter, soak and decoct the filter residue repeatedly, mix the two filtrates, evaporate and concentrate, and freeze-dry to obtain the plant extract.

[0013] The third object of the present invention is to provide the use of the above plant extract or the plant extract prepared by the above method in the preparation of drugs for preventing / treating Helicobacter pylori.

[0014] The roles played by each raw material of the present invention in the present invention are as follows:

[0015] Dandelion has a strong killing effect on Helicobacter pylori (HP), and also has the effects of clearing away heat, detoxifying, reducing swelling and dispersing nodules.

[0016] Pomegranate peel has an inhibitory effect on Helicobacter pylori (HP), which may be related to the tannins, pigments and trace alkaloids it contains. Pomegranate peel can nourish the stomach and strengthen the spleen, and has a good therapeutic effect on patients with long-term indigestion and stomach bloating and stomach pain.

[0017] Fennel warms the kidney and dispels cold, harmonizes the stomach and regulates qi, and mainly contains volatile oils, fennel ketones, terpenes, flavonoids and other active ingredients, which can inhibit the growth of Helicobacter pylori (HP), stimulate gastrointestinal nerves and blood vessels, promote the secretion of digestive juices, increase gastrointestinal motility, and have the effect of strengthening the stomach and promoting qi, which helps to relieve stomach cramps and relieve pain, while preventing other components from being too cold and damaging the liver.

[0018] The invention optimizes the combination of three natural products, has a synergistic effect, the optimal ratio can inhibit Helicobacter pylori, and at the same time strengthens the stomach and promotes gastrointestinal peristalsis, not only achieving the effect of treatment, but also achieving the effect of nourishing the stomach and preventing recurrence. The raw materials are simple and easy to obtain, high-concentration gastric acid is an environment suitable for the reproduction of Helicobacter pylori, fennel and pomegranate peel can reduce the secretion of gastric acid, can inhibit the growth and reproduction of Helicobacter pylori to a certain extent, change the conditions for the survival of Helicobacter pylori, and improve the clearance rate and eradication rate of Helicobacter pylori.

[0019] The preparation method of the present invention adopts grinding combined with extraction technology to enable effective active components to be fully dissolved, and the freeze-drying method can retain the active ingredients compared with the common drying method, so that the pharmaceutical composition takes effect quickly and has reliable efficacy. DETAILED DESCRIPTION

[0020] According to the information contained in this application, various changes can be easily made to the precise description of the present invention for those skilled in the art.It should be understood that the scope of the present invention is not limited to defined processes, properties or components, because these embodiments and other descriptions are only for illustrating specific aspects of the present invention.

[0021] In order to better understand the present invention but not to limit the scope of the present invention, all the numbers used in this application to express the amount, percentage, and other numerical values ​​should be understood as modified by the word "about" in all cases. Therefore, unless otherwise specified, the numerical parameters listed in the specification are approximate values, which may be changed according to the different ideal properties attempted to be obtained. Each numerical parameter should at least be regarded as obtained based on the reported significant figures and by conventional rounding methods.

[0022] The equipment and raw materials used in the present invention can be purchased from the market or are commonly used in the art. The methods in the following embodiments are all conventional methods in the art unless otherwise specified.

[0023] Example 1

[0024] Prepare the following raw materials and weight ratios:

[0025] Dandelion 40g Pomegranate peel 15g Fennel 25g

[0026] The preparation method of the present invention comprises the following steps: taking dried pomegranate peel, fennel and dandelion, crushing them into powder using a grinding device; extracting by ethanol reflux, filtering, re-refluxing extraction of medicinal residues, and drying by a freeze dryer to obtain powder, thereby obtaining the plant extract.

[0027] Example 2

[0028] Prepare the following raw materials and weight ratios:

[0029] Dandelion 30g Pomegranate peel 10g Fennel 22g

[0030] The preparation method of the present invention comprises the following steps: taking dried pomegranate peel, fennel and dandelion, crushing them into powder using a grinding device; extracting by ethanol reflux, filtering, re-refluxing extraction of medicinal residues, and drying by a freeze dryer to obtain powder, thereby obtaining the plant extract.

[0031] Example 3

[0032] Prepare the following raw materials and weight ratios:

[0033] Dandelion 35g Pomegranate peel 12g Fennel 23g

[0034] The preparation method of the present invention comprises the following steps: taking dried pomegranate peel, fennel and dandelion, crushing them into powder using a grinding device; extracting by ethanol reflux, filtering, re-refluxing extraction of medicinal residues, and drying by a freeze dryer to obtain powder, thereby obtaining the plant extract.

[0035] Example 4

[0036] Prepare the following raw materials and weight ratios:

[0037] Dandelion 40g Pomegranate peel 15g Fennel 25g

[0038] The preparation method of the present invention comprises the following steps: taking pomegranate peel, fennel and dandelion respectively, adding appropriate amount of water to soak for 30 minutes and then decocting for 30 minutes, filtering, repeatedly soaking and decocting the filter residue, mixing the two filtrates, evaporating and concentrating, and freeze-drying to obtain the plant extract.

[0039] Example 5

[0040] Prepare the following raw materials and weight ratios:

[0041] Dandelion 30g Pomegranate peel 10g Fennel 22g

[0042] The preparation method of the present invention comprises the following steps: taking pomegranate peel, fennel and dandelion respectively, adding appropriate amount of water to soak for 30 minutes and then decocting for 30 minutes, filtering, repeatedly soaking and decocting the filter residue, mixing the two filtrates, evaporating and concentrating, and freeze-drying to obtain the plant extract.

[0043] Example 6

[0044] Prepare the following raw materials and weight ratios:

[0045] Dandelion 35g Pomegranate peel 12g Fennel 23g

[0046] The preparation method of the present invention comprises the following steps: taking pomegranate peel, fennel and dandelion respectively, adding appropriate amount of water to soak for 30 minutes and then decocting for 30 minutes, filtering, repeatedly soaking and decocting the filter residue, mixing the two filtrates, evaporating and concentrating, and freeze-drying to obtain the plant extract.

[0047] Example 7 Preparation of anti-HP drugs

[0048] (1) Tablets

[0049] The plant extracts prepared in Examples 1 to 4 were mixed with an appropriate amount of dextrin to prepare granules, an appropriate amount of magnesium stearate was added, the mixture was mixed and evenly mixed, and then compressed to prepare tablets.

[0050] (2) Capsules

[0051] The plant extracts prepared in Examples 1 to 4 were filled into capsules to prepare capsules.

[0052] Experimental example

[0053] (1) After the mice were adaptively raised for one week, they were randomly divided into a normal group and a modeling group according to the random number table method. The modeling group was infected with HP bacteria solution (1×10 9 CFU / ml, 0.2ml / mouse), infected once every other day, a total of 5 times, colonized for 2 weeks after infection.

[0054] (2) Administration: The modeling group was divided into a model group, a standard quadruple group, and a high-, medium-, and low-dose Chinese medicine group, with 8 mice in each group. The mice in the normal group and the model group were gavaged with normal saline every day. The Chinese medicine group was gavaged with different concentrations of Chinese medicine composition (400, 200, 100 mg / kg), and the standard quadruple group was gavaged with a quadruple drug suspension. The administration was once a day for a total of 2 weeks.

[0055] Among them, the standard quadruple drugs are: esomeprazole enteric-coated capsules, amoxicillin clavulanate potassium tablets, clarithromycin tablets, and colloidal pectin bismuth dry suspension. The reference adult (60kg) dosage per dose is: esomeprazole 20mg, amoxicillin 1000mg, clarithromycin 500mg, colloidal pectin bismuth 300mg, and the mouse dosage is converted according to the body surface area of ​​60kg adults. The equivalent daily dose for mice is 262.5mg / kg. First, grind and mix each drug in proportion and dissolve it in distilled water before gavage.

[0056] The Chinese medicine group used the plant extract prepared in Example 1.

[0057] (3) One hour after the last administration, the mice were killed by dislocation of the neck, and the whole stomach was removed by laparotomy. The stomach was cut open along the greater curvature, and the gastric mucosa was exposed after the stomach contents were flushed with saline. The antrum and body mucosa were observed with the naked eye to see if there was erosion, bleeding, or ulceration. Gastric mucosal tissues were taken from the antrum, body, and fundus for rapid urease test. Urease is an important colonization factor and virulence factor of HP. The principle is that bacteria produce urease, which decomposes urea. The ammonia produced will change the color of the reagent, thereby judging whether there is Helicobacter pylori infection.

[0058] Table 1 Results of rapid urease test in mouse gastric mucosal tissue

[0059] Group quantity HP(-) HP(+) HP removal rate Normal group 8 8 0 - Model Group 8 0 8 - Standard quadruple set 8 8 0 100％ Chinese medicine group (400mg / kg) 8 7 1 87.5％ Chinese medicine group (200mg / kg) 8 6 2 75％ Chinese medicine group (100mg / kg) 8 4 4 50％

[0060] As can be seen from Table 1, the plant extract of the present invention has a strong inhibitory and killing effect on Helicobacter pylori. Long-term use of the medicine of the present invention has no adverse reactions and toxic side effects. Compared with the current Western medicine treatment of this disease, the treatment cost is reduced by more than 60%, the efficacy is milder, and the toxic side effects are avoided. It is an ideal Chinese herbal medicine preparation for treating HP infected gastropathy.

[0061] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A plant extract, characterized in that The plant extracts include dandelion, fennel and pomegranate peel.

2. A plant extract, characterized in that The plant extract comprises 30-40 parts by weight of dandelion, 22-25 parts by weight of fennel and 10-15 parts by weight of pomegranate peel.

3. The method for preparing a plant extract according to claim 1, characterized in that: The steps include: S1. Take dried pomegranate peel, fennel and dandelion and crush them into powder using a grinding device; S2, extracting by reflux, filtering, extracting by reflux again the residue, and drying by freeze drying to obtain powder, thereby obtaining the plant extract.

4. The preparation method according to claim 3, wherein the extraction is performed by reflux with water, ethanol or ethanol-water solution.

5. The method for preparing the plant extract according to claim 1, characterized in that: The steps include: Take pomegranate peel, fennel and dandelion respectively, add appropriate amount of water to soak for 30 minutes, then decoct for 30 minutes, filter, soak and decoct the filter residue repeatedly, mix the two filtrates, evaporate and concentrate, and freeze-dry to obtain the plant extract.

6. Use of the plant extract according to any one of claims 1 to 2 or the plant extract obtained according to the preparation method according to any one of claims 3 to 5 in the preparation of a drug for preventing / treating Helicobacter pylori.