Method capable of improving extraction rate of protein polypeptide from sheep embryo muscle
Through multi-step processing methods, including cleaning, freezing, ultrafine crushing and hydrolysis, the problem of inconsistent raw material quality in the production of protein peptides for sheep embryo extract is solved, the extraction rate and purity are improved, and the production quality of beauty and health care products is ensured.
Patent Information
- Application Number
- CN202411455647.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-18
- Publication Date
- 2025-01-03
AI Technical Summary
In the existing production process of sheep embryo extraction protein polypeptides, large-scale production leads to inconsistent raw material quality, resulting in unstable extraction rate and purity, which reduces the production quality of beauty and health care products.
Multi-step treatment methods are adopted, including multiple cleaning of raw materials, freezing treatment, ultra-fine crushing, hydrolysis and spray drying, ensuring efficient extraction of sheep embryo raw materials and the purity of protein polypeptides. Specific steps include: raw material preparation, crushing, ultra-fine crushing, filtration and extraction, preparation of hydrolysate, mixing and regulation and drying collection, and minimize protein loss and impurity content by controlling temperature, pH and heating.
It improves the extraction rate and purity of protein polypeptides in sheep embryo muscles, ensures the quality and efficiency of the extracts, reduces raw material losses, and improves production income.
Smart Images
Figure CN119242746A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of protein polypeptide extraction, in particular to a method for improving the extraction rate of protein polypeptide from sheep embryonic muscle. Background Art
[0002] After the protein ingested by humans is acted upon by digestive enzymes, it is not absorbed mainly in the form of amino acids, but rather in the form of peptides. Some peptides can not only provide the nutrients needed for human growth and development, but also have multiple physiological functions, such as improving and enhancing the transport and absorption of minerals, anti-bacteria and anti-virus, improving immunity, anti-oxidation, lowering cholesterol, anti-cancer, scavenging free radicals and anti-aging.
[0003] Scientific research has shown that sheep embryo extract contains a variety of active ingredients that can regulate the functions of human tissues and organs, including superoxide dismutase (SOD), epidermal growth factor (EGF), hyaluronic acid stimulating factor (HASF) and basic fibroblast growth factor (bFGF), which have the effect of beautifying the skin. Therefore, sheep embryo extract has become one of the important raw materials for the production of beauty and health products.
[0004] However, in the existing production process of extracting protein peptides from sheep embryos, due to the large-scale production method of the enterprise, the quality of the sheep embryo raw materials cannot be guaranteed to be completely consistent, which makes different batches of sheep embryo extracts have different protein peptide extraction rates and different impurities, thereby reducing the purity of the protein peptide solution extracted from the sheep embryo raw materials and reducing the production quality of subsequent beauty and health products. In view of this, we proposed a method for improving the extraction rate of protein peptides from sheep embryo muscles. Summary of the invention
[0005] The object of the present invention is to provide a method for improving the extraction rate of protein polypeptides from sheep embryonic muscles, so as to solve the problems raised in the above-mentioned background technology.
[0006] To achieve the above object, the present invention provides the following technical solution: a method for improving the extraction rate of protein polypeptides from sheep embryonic muscle, comprising the following steps: Step 1, raw material preparation: take sheep embryos from quarantined healthy disease-free ewes with a gestation period of 2 to 3 months, and ensure that the sheep embryo raw materials are extracted within 12 hours, wash the sheep embryo raw materials with purified water and then freeze them to obtain frozen sheep embryos and sheep embryo washing solution; Step 2, crushing: crushing the frozen sheep embryo obtained in step 1 by a crusher, and ensuring that the operating environment of the crusher is at 0-5° C., and obtaining crude sheep embryo frozen powder after crushing; Step 3, ultrafine grinding: placing the crude sheep embryo frozen powder obtained in step 2 in a -30°C environment and freezing for 30 minutes, then placing the frozen crude sheep embryo frozen powder in an ultrafine grinder for ultrafine grinding, and finally obtaining fine sheep embryo frozen powder; Step 4, filtering and extracting: adding the fine sheep embryo frozen powder obtained in step 3 into a mixing container, and pouring the sheep embryo washing solution obtained in step 1 into it, and adding distilled water at a mass ratio of 1:10 to mix, centrifuging the mixed solution in the mixing container by a centrifuge, and filtering the suspension after the centrifugation to obtain a filtrate and a precipitate; Step 5, preparing a hydrolyzate: taking the precipitate obtained in step 4 into a hydrolysis container, adding an appropriate amount of protease into the hydrolysis container, and adjusting the pH value in the hydrolysis container to between 7.0 and 9.0, and controlling the temperature range of the hydrolysis container to 50° C.-60° C. by a temperature control device, cooling to room temperature after the hydrolysis is completed, and filtering to obtain a sheep embryo hydrolyzate; Step 6: Mixing and adjusting: Mix the filtrate obtained in step 4 with the sheep embryo hydrolyzate obtained in step 5, and adjust the pH value of the mixed solution to neutral to obtain sheep embryo essence stock solution; Step 7, drying and collection: pump the sheep embryo extract stock solution obtained in step 6 into a spray dryer to spray out fine droplets, and heat the fine droplets to evaporate and dry them quickly to obtain the protein polypeptide extract powder for collection.
[0007] Preferably, the sheep embryo raw material in the step one is cleaned with purified water before processing, and the cleaning process is divided into two steps. The first step is to continuously spray purified water onto the surface of the sheep embryo raw material to wash away impurities such as excess tissue and blood on the surface of the sheep embryo. The second step is to preserve the cleaning liquid obtained after cleaning the sheep embryo raw material to be used as a mixed liquid in the subsequent mixing step, thereby reducing the loss of raw materials and avoiding the impurities on the surface of the initial sheep embryo raw material from affecting the subsequent extraction operation, thereby improving the extraction accuracy of the sheep embryo extract.
[0008] Preferably, the freezing temperature of the sheep embryo raw material in the step 1 is set to between -45 and -50°C, and the temperature of the sheep embryo raw material is cooled in a stepwise manner during freezing, and the cooling time is set to 50 to 60 minutes, so that the sheep embryo raw material reaches the degree of freeze-drying after freezing without excessively damaging its internal structure, thereby ensuring its freshness, facilitating transportation and transshipment, ensuring its activity, and adopting a step-by-step cooling method to effectively avoid damage to the sheep embryo raw material, thereby affecting the subsequent protein and polypeptide extraction rate, and the sheep embryo cleaning solution is sealed and stored at room temperature to avoid being affected by other environmental factors during processing and to avoid affecting the preparation of the subsequent mixed solution.
[0009] Preferably, when the frozen sheep embryo is crushed in the step 2, a filtering device is used to filter the primary crushed material, and the filtering device adopts a filter with a mesh diameter of 5 mm, so that the fineness of the coarse frozen sheep embryo powder obtained in the step 2 is less than 5 mm, thereby facilitating the subsequent ultrafine crushing operation.
[0010] Preferably, the ultrafine grinding in the step 3 is carried out for multiple times. First, the crude sheep embryo frozen powder obtained in the step 2 is divided into several portions, and the ultrafine grinding operation is performed on one portion of the crude sheep embryo powder each time. The obtained fine sheep embryo frozen powder is placed in a freezing device for freezing, and then the output after each ultrafine grinding is mixed with the fine sheep embryo frozen powder obtained before, and finally all the mixed outputs are ultrafinely ground again to obtain uniformly mixed fine sheep embryo frozen powder, thereby ensuring the ultrafine grinding effect of the crude sheep embryo powder, effectively reducing the fineness of the sheep embryo powder, thereby facilitating the subsequent hydrolysis operation and improving the efficiency of filtering out impurities in purified water.
[0011] Preferably, when mixing in step 4, an ethanol solution is added in a volume ratio of 1:20 to distilled water, and the mixture is first shaken and then centrifuged, and the shaking-centrifugation-standing-precipitation steps are repeated more than three times until there is no obvious precipitation in the mixed solution, so as to effectively extract the precipitate in the mixed solution of fine sheep embryo frozen powder and ensure the dissolution effect of the mixed solution.
[0012] Preferably, during the centrifugation operation in step 4, the ambient temperature is ensured to be between 0 and 5°C, and the temperature of the added distilled water is also between 0 and 5°C, and the distilled water is added in three steps, with the volumes of purified water added three times being 20%, 30%, and 50% of the total added volume, respectively, to ensure that the fine sheep embryo frozen powder has a better dissolution effect and will not freeze or deteriorate, thereby affecting the subsequent mixing operation.
[0013] Preferably, NaOH solution is used for pH adjustment in step five, and the concentration of the NaOH solution is 1-3 mol / L, so that the pH value of the mixed solution in the hydrolysis container can be quickly adjusted while ensuring that as little pH adjustment solution as possible is added, so that the final result is more accurate, and a water bath heater is provided outside the hydrolysis container to make the temperature increase in the hydrolysis container more balanced and stable, and to maintain a good temperature maintenance effect, thereby ensuring the activity of the protease in the hydrolysis operation, thereby more efficiently destroying the cell wall of the cell, thereby releasing the protein polypeptide in the cell.
[0014] Preferably, a centrifuge is used for mixing in step six, and the speed of the centrifuge is set to 5000r / min, and the centrifugal operation time is not less than 10min and not more than 15min, so that the mixing between the sheep embryo hydrolyzate and the filtrate is more uniform, and the suspension of the mixed solution is removed before finally obtaining the sheep embryo essence stock solution, so as to remove impurities in the mixed solution to the maximum extent and ensure the purity of the extracted protein polypeptide.
[0015] Preferably, the heating method in the spray dryer is low-temperature heating, and the temperature range in the spray dryer is 40°C to 80°C to avoid affecting the activity of the protein polypeptide during the spray drying process, thereby reducing the use effect of the extracted protein polypeptide.
[0016] Compared with the prior art, the present invention provides a method for improving the extraction rate of protein polypeptides from sheep embryonic muscle, which has the following beneficial effects: 1. The method for improving the extraction rate of protein polypeptides from sheep embryo muscle comprises the following steps: washing sheep embryo raw materials for many times, ultrafine grinding after extracting coarse sheep embryo frozen powder, mixing hydrolyzate prepared from fine sheep embryo frozen powder and washing solution, and centrifugation and removal of suspension before obtaining sheep embryo essence stock solution, thereby extracting more protein polypeptides while minimizing protein loss, ensuring the efficiency and purity of protein polypeptides extracted from sheep embryo muscle raw materials, thereby improving the income of the preparation method and reducing the loss of raw materials.
[0017] 2. The method for improving the extraction rate of protein polypeptides from sheep embryo muscles comprises the following steps: performing batch multiple pulverization during the ultrafine pulverization process, and mixing the pulverized outputs and then performing the ultrafine pulverization step again, thereby ensuring the ultrafine pulverization effect of the coarse sheep embryo powder, effectively reducing the fineness of the sheep embryo powder, thereby facilitating the subsequent hydrolysis operation, improving the efficiency of filtering out impurities in purified water, and reducing the impurity content in the sheep embryo stock solution.
[0018] 3. The method for improving the extraction rate of protein peptides from sheep embryo muscles is to avoid the use of a water bath to stably heat the hydrolysis container and to add purified water in batches during the hydrolysis process to ensure that the fine sheep embryo frozen powder has a good dissolution effect and will not freeze or deteriorate, thereby affecting the subsequent mixing operation. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 Schematic diagram of the protein polypeptide extraction steps of the present invention. DETAILED DESCRIPTION
[0020] like Figure 1As shown, the present invention provides a technical solution: a method for improving the extraction rate of protein polypeptides from sheep embryonic muscle, 1. comprising the following steps: Step 1, raw material preparation: take sheep embryos from quarantined healthy disease-free ewes with a gestation period of 2 to 3 months, and ensure that the sheep embryo raw materials are extracted within 12 hours, wash the sheep embryo raw materials with purified water and then freeze them to obtain frozen sheep embryos and sheep embryo washing solution; Step 2, crushing: the frozen sheep embryos obtained in step 1 are collected and frozen in time by a crusher, and then crushed uniformly, and the operating environment of the crusher is ensured to be at 0-5° C., and crude sheep embryo frozen powder is obtained after crushing; Step 3, ultrafine grinding: placing the crude sheep embryo frozen powder obtained in step 2 in a -30°C environment and freezing for 30 minutes, then placing the frozen crude sheep embryo frozen powder in an ultrafine grinder for ultrafine grinding, and finally obtaining fine sheep embryo frozen powder; Step 4, filtering and extracting: adding the fine sheep embryo frozen powder obtained in step 3 into a mixing container, and pouring the sheep embryo washing solution obtained in step 1 into it, and adding distilled water at a mass ratio of 1:10 to mix, centrifuging the mixed solution in the mixing container by a centrifuge, and filtering the suspension after the centrifugation to obtain a filtrate and a precipitate; Step 5, preparing a hydrolyzate: taking the precipitate obtained in step 4 into a hydrolysis container, adding an appropriate amount of protease into the hydrolysis container, and adjusting the pH value in the hydrolysis container to between 7.0 and 9.0, and controlling the temperature range of the hydrolysis container to 50° C.-60° C. by a temperature control device, cooling to room temperature after the hydrolysis is completed, and filtering to obtain a sheep embryo hydrolyzate; Step 6: Mixing and adjusting: Mix the filtrate obtained in step 4 with the sheep embryo hydrolyzate obtained in step 5, and adjust the pH value of the mixed solution to neutral to obtain sheep embryo essence stock solution; Step 7, drying and collection: pump the sheep embryo extract stock solution obtained in step 6 into a spray dryer to spray out fine droplets, and heat the fine droplets to evaporate and dry them quickly to obtain the protein polypeptide extract powder for collection.
[0021] In one embodiment of the present invention, the sheep embryo raw material in step one is cleaned with purified water before processing, and the cleaning process is divided into two steps. The first step is to continuously spray purified water onto the surface of the sheep embryo raw material to wash away impurities such as excess tissue and blood on the surface of the sheep embryo. The second step is to preserve the cleaning solution obtained after cleaning the sheep embryo raw material to be used as a mixed solution in the subsequent mixing step, thereby reducing the loss of raw materials and avoiding the impurities on the surface of the initial sheep embryo raw material from affecting the subsequent extraction operation, thereby improving the extraction accuracy of the sheep embryo extract. Furthermore, the freezing temperature of the sheep embryo raw material in step one is The temperature is set between -45 and -50°C, and the sheep embryo raw material is cooled in a step-by-step manner during freezing, and the cooling time is set to 50 to 60 minutes, so that the sheep embryo raw material reaches the degree of freeze-drying after freezing without excessively destroying its internal structure, ensuring its freshness, facilitating transportation and transshipment, ensuring its activity, and adopting a step-by-step cooling method to effectively avoid damage to the sheep embryo raw material and affect the subsequent protein and polypeptide extraction rate, and the sheep embryo cleaning solution is sealed and stored at room temperature to avoid it being affected by other environmental factors during the processing and to avoid it affecting the preparation of the subsequent mixed solution.
[0022] In addition, when the frozen sheep embryo is crushed in step 2, a filtering device is used to filter the primary crushed material, and the filtering device adopts a filter with a mesh diameter of 5 mm, so that the fineness of the coarse frozen sheep embryo powder obtained in step 2 is less than 5 mm, thereby facilitating the subsequent ultrafine grinding operation.
[0023] At the same time, the ultrafine grinding in step 3 is carried out for multiple times. First, the crude sheep embryo frozen powder obtained in step 2 is divided into several portions, and the ultrafine grinding operation is performed on one portion of the crude sheep embryo powder each time. The obtained fine sheep embryo frozen powder is placed in a freezing device for freezing, and then the output after each ultrafine grinding is mixed with the fine sheep embryo frozen powder obtained before, and finally all the mixed outputs are ultrafinely ground again to obtain uniformly mixed fine sheep embryo frozen powder, thereby ensuring the ultrafine grinding effect of the crude sheep embryo powder, effectively reducing the fineness of the sheep embryo powder, thereby facilitating the subsequent hydrolysis operation and improving the efficiency of filtering out impurities in purified water.
[0024] In an embodiment of the present invention, when mixing in step 4, ethanol solution is added in a volume ratio of 1:20 to distilled water, and the mixture is first shaken and then centrifuged, and the shaking-centrifugation-standing-precipitation steps are repeated more than three times until there is no obvious precipitation in the mixed solution, so as to effectively extract the precipitate in the mixed solution of the fine sheep embryo frozen powder and ensure the dissolution effect of the mixed solution. At the same time, when centrifuging in step 4, the ambient temperature is ensured to be between 0 and 5°C, and the temperature of the added distilled water is also between 0 and 5°C, and the addition of distilled water is carried out in three steps, and the volumes of purified water added three times are 20%, 30%, and 50% of the total added volume, respectively, to ensure that the dissolution effect of the fine sheep embryo frozen powder is better, and freezing or deterioration will not occur, which will affect the subsequent mixing operation.
[0025] In the present invention, NaOH solution is used for pH adjustment in step 5, and the concentration of NaOH solution is 1-3 mol / L, so that the pH value of the mixed solution in the hydrolysis container can be quickly adjusted while ensuring that as little pH adjustment solution is added as possible, so that the final result is more accurate, and a water bath heater is arranged outside the hydrolysis container, so that the temperature increase in the hydrolysis container is more balanced and stable, and a good temperature maintenance effect can be maintained, thereby ensuring the activity of the protease in the hydrolysis operation, thereby more efficiently destroying the cell wall of the cell, thereby releasing the protein polypeptide in the cell, and specifically, centrifuge mixing is used for mixing in step 6, and the speed of the centrifuge is set to 5000r / min, and the centrifugal operation time is not less than 10min and not more than 15min, so that the mixing between the sheep embryo hydrolyzate and the filtrate is more uniform, and the suspension of the mixed solution is removed before finally obtaining the sheep embryo element stock solution, so as to remove impurities in the mixed solution to the maximum extent, and ensure the purity of the extracted protein polypeptide.
[0026] It is worth noting that the heating method in the spray dryer is low-temperature heating, and the temperature range in the spray dryer is 40°C to 80°C, so as to avoid affecting the activity of protein peptides during the spray drying process, thereby reducing the use effect of the extracted protein peptides.
[0027] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.
Claims
1. A method for improving the extraction rate of protein polypeptides from sheep embryonic muscle, characterized in that: The following steps are involved: Step 1, raw material preparation: take sheep embryos from quarantined healthy disease-free ewes with a gestation period of 2 to 3 months, and ensure that the sheep embryo raw materials are extracted within 12 hours, wash the sheep embryo raw materials with purified water and then freeze them to obtain frozen sheep embryos and sheep embryo washing solution; Step 2, crushing: crushing the frozen sheep embryo obtained in step 1 by a crusher, and ensuring that the operating environment of the crusher is at 0-5° C., and obtaining crude sheep embryo frozen powder after crushing; Step 3, ultrafine grinding: placing the crude sheep embryo frozen powder obtained in step 2 in a -30°C environment and freezing for 30 minutes, then placing the frozen crude sheep embryo frozen powder in an ultrafine grinder for ultrafine grinding, and finally obtaining fine sheep embryo frozen powder; Step 4, filtering and extracting: adding the fine sheep embryo frozen powder obtained in step 3 into a mixing container, and pouring the sheep embryo washing solution obtained in step 1 into it, and adding distilled water at a mass ratio of 1:10 to mix, centrifuging the mixed solution in the mixing container by a centrifuge, and filtering the suspension after the centrifugation to obtain a filtrate and a precipitate; Step 5, preparing a hydrolyzate: taking the precipitate obtained in step 4 into a hydrolysis container, adding an appropriate amount of protease into the hydrolysis container, and adjusting the pH value in the hydrolysis container to between 7.0 and 9.0, and controlling the temperature range of the hydrolysis container to 50° C.-60° C. by a temperature control device, cooling to room temperature after the hydrolysis is completed, and filtering to obtain a sheep embryo hydrolyzate; Step 6: Mixing and adjusting: Mix the filtrate obtained in step 4 with the sheep embryo hydrolyzate obtained in step 5, and adjust the pH value of the mixed solution to neutral to obtain sheep embryo essence stock solution; Step 7, drying and collection: pump the sheep embryo extract stock solution obtained in step 6 into a spray dryer to spray out fine droplets, and heat the fine droplets to evaporate and dry them quickly to obtain the protein polypeptide extract powder for collection.
2. A method for improving the extraction rate of protein polypeptides from sheep embryonic muscle according to claim 1, characterized in that: The sheep embryo raw material in step one is cleaned with purified water before processing, and the cleaning process is divided into two steps. The first step is to continuously spray purified water on the surface of the sheep embryo raw material to wash away impurities such as excess tissue and blood on the surface of the sheep embryo. The second step is to preserve the cleaning liquid obtained after cleaning the sheep embryo raw material to be used as a mixed liquid in the subsequent mixing step.
3. A method for improving the extraction rate of protein polypeptides from sheep embryo muscle according to claim 1, characterized in that: The freezing temperature of the sheep embryo raw material in the step 1 is set to between -45 and -50°C, and the temperature of the sheep embryo raw material is gradually reduced during freezing, the cooling time is set to 50 to 60 minutes, and the sheep embryo cleaning solution is sealed and stored at room temperature.
4. A method for improving the extraction rate of protein polypeptides from sheep embryo muscle according to claim 1, characterized in that: When the frozen sheep embryo is crushed in the step 2, a filtering device is used to filter the primary crushed material, and the filtering device uses a filter with a mesh diameter of 5 mm, so that the fineness of the coarse frozen sheep embryo powder obtained in the step 2 is less than 5 mm.
5. A method for improving the extraction rate of protein polypeptides from sheep embryo muscle according to claim 1, characterized in that: The ultrafine grinding in the step 3 is performed multiple times. First, the crude sheep embryo frozen powder obtained in the step 2 is divided into several portions, and the ultrafine grinding operation is performed on one portion of the crude sheep embryo powder each time. The obtained fine sheep embryo frozen powder is placed in a freezing device for freezing, and then the output after each ultrafine grinding is mixed with the fine sheep embryo frozen powder obtained before. Finally, all the mixed outputs are ultrafinely ground again to obtain uniformly mixed fine sheep embryo frozen powder.
6. A method for improving the extraction rate of protein polypeptides from sheep embryo muscle according to claim 1, characterized in that: When mixing in step 4, add ethanol solution in a volume ratio of 1:20 to distilled water, shake well first and then centrifuge, and repeat the shaking-centrifugation-standing-precipitation steps for more than three times until there is no obvious precipitation in the mixed solution.
7. A method for improving the extraction rate of protein polypeptides from sheep embryonic muscle according to claim 1, characterized in that: During the centrifugation operation in step 4, the ambient temperature is ensured to be between 0 and 5° C., and the temperature of the added distilled water is also between 0 and 5° C., and the addition of distilled water is carried out in three steps, with the volumes of purified water added three times being 20%, 30%, and 50% of the total added volume respectively.
8. A method for improving the extraction rate of protein polypeptides from sheep embryo muscle according to claim 1, characterized in that: In the step 5, NaOH solution is used for pH adjustment, and the concentration of the NaOH solution is 1-3 mol / L, and a water bath heater is arranged outside the hydrolysis container.
9. A method for improving the extraction rate of protein polypeptides from sheep embryo muscle according to claim 1, characterized in that: In the step 6, a centrifuge is used for mixing, and the speed of the centrifuge is set to 5000 r / min, and the centrifugal operation time is not less than 10 minutes and not more than 15 minutes.
10. A method for improving the extraction rate of protein polypeptides from sheep embryonic muscle according to claim 1, characterized in that: The heating method in the spray dryer is low temperature heating, and the temperature range in the spray dryer is 40°C to 80°C.