Fingerprint detection method for Yankanning tablets

Abstract

The invention is applicable to the technical field of detection of Yankingning tablets, and particularly relates to a fingerprint detection method for Yankingning tablets, which comprises the following steps: preparing a Yankingning tablet test solution; preparing a reference solution; after the chromatographic conditions are matched with the system applicability, respectively injecting 5 microliters of the test solution and 5 microliters of the reference solution into a liquid chromatograph for determination to obtain a fingerprint spectrum and a reference fingerprint spectrum of the Yankingning tablets; and analyzing according to the fingerprint spectrum of the Yankingning tablet and the control fingerprint spectrum to obtain similarity information. According to the method, through a standardized detection process, various components in the Yankingning tablet are detected at the same time, the basis of pharmacodynamic substances of the Yankingning tablet is comprehensively reflected, and the quality of the Yankingning tablet can be comprehensively evaluated; by comprehensively analyzing the similarity between the fingerprint spectrum of the Yankingning tablets and the control fingerprint spectrum, the quality and consistency of the Yankingning tablets can be comprehensively evaluated, and the stability and reliability of quality evaluation of the Yankingning tablets are improved.

Description

Technical Field

[0001] The present application belongs to the technical field of Yankening tablet detection, and in particular, relates to a Yankening tablet fingerprint detection method. Background Art

[0002] Yankening tablets are a commonly used Chinese patent medicine, widely used to treat diseases caused by inflammation, such as pharyngitis, tonsillitis, etc. In order to ensure the quality and efficacy of Yankening tablets, it is very necessary to accurately analyze its ingredients qualitatively and quantitatively. In recent years, with the development of science and technology, fingerprint technology has been widely used because it can fully reflect the complexity of Chinese medicine ingredients. Fingerprint technology can qualitatively and quantitatively analyze the various active ingredients in Yankening tablets through high-performance liquid chromatography (HPLC) and other means, thus providing a scientific and accurate means of quality control.

[0003] Traditional fingerprint detection methods for Yankening tablets often rely on the detection of a single component, which is time-consuming and difficult to quickly and comprehensively reflect the complex components and efficacy of Yankening tablets. Summary of the invention

[0004] The embodiment of the present application provides a fingerprint spectrum detection of Yankening tablets, which can solve the problem that the traditional fingerprint spectrum detection method of Yankening tablets often relies on the detection of a single component, is time-consuming, and is difficult to quickly and comprehensively reflect the complex components and efficacy of Yankening tablets.

[0005] In the first aspect, the present application embodiment provides a method for detecting a fingerprint of Yankening tablets, comprising:

[0006] Prepare Yankening tablets test solution;

[0007] Prepare reference solution;

[0008] After the chromatographic conditions are matched with the system suitability, 5 μl of the Yankening Tablets test solution and the reference solution are respectively injected into the liquid chromatograph for determination to obtain the Yankening Tablets fingerprint spectrum and the reference fingerprint spectrum;

[0009] The fingerprint spectrum of the Yankening tablets and the reference fingerprint spectrum are analyzed to obtain similarity information.

[0010] The above technical solutions in the embodiments of the present application have at least the following technical effects:

[0011] The fingerprint detection method of Yankening tablets provided in the present application is firstly prepared by preparing a test solution of Yankening tablets and a reference solution; after the chromatographic conditions are matched with the system suitability, 5 μl of each of the test solution of Yankening tablets and the reference solution are injected into the liquid chromatograph for determination to obtain the fingerprint of Yankening tablets and the reference fingerprint; finally, the fingerprint of Yankening tablets and the reference fingerprint are analyzed to obtain similarity information. This method uses a standardized detection process, compares with the reference solution, and simultaneously detects multiple components in Yankening tablets, comprehensively reflects the basis of its effective substances, and helps to comprehensively evaluate the quality of Yankening tablets; by comprehensively analyzing the similarity between the fingerprint of Yankening tablets and the reference fingerprint, the quality and consistency of Yankening tablets can be comprehensively evaluated, and the stability and reliability of the quality evaluation of Yankening tablets can be improved.

[0012] In a possible implementation of the first aspect, the preparation of the Yankening tablet test solution includes:

[0013] Take 10 Yankening tablets, remove the coating and grind into powder, take 0.3g Yankening powder and put it into a stoppered conical bottle;

[0014] Then, add 50 ml of 80% pure methanol into the stoppered conical flask to obtain a first mixed solution, and weigh the mixture;

[0015] The first mixed liquid is ultrasonically treated for 30 minutes to obtain a second mixed liquid. The second mixed liquid is cooled to room temperature and then weighed. The weight of the second mixed liquid is supplemented with methanol with a purity of 80%, and then the second mixed liquid is homogenized and filtered to obtain a test solution of Yankening tablets; wherein the power of the ultrasonic treatment is 480 W and the frequency is 40 kHz.

[0016] In a possible implementation of the first aspect, preparing the reference solution includes:

[0017] Weigh preset weights of berberine hydrochloride reference substance, baicalin reference substance, aloe-emodin reference substance, rhein acid reference substance, rhein reference substance and chrysophanol reference substance, and use 80% pure methanol to make a solution containing 10 μg of berberine hydrochloride, 55 μg of baicalin, 4 μg of aloe-emodin, 10 μg of rhein acid, 2 μg of rhein and 4 μg of chrysophanol per 1 ml to obtain a reference substance solution.

[0018] In a possible implementation manner of the first aspect, the chromatographic conditions are matched with the system suitability, including:

[0019] Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A and 0.2% phosphoric acid solution is used as mobile phase B for gradient elution; under preset detection conditions, the theoretical plate number is not less than 2000 calculated based on the baicalin peak.

[0020] In a possible implementation of the first aspect, the performing gradient elution includes:

[0021] When the time period is 0 to 70 minutes: the volume percentage of mobile phase A increases from 30% to 65%; the volume percentage of mobile phase B decreases from 70% to 35%;

[0022] When the time period is 70 to 85 minutes: the volume percentage of mobile phase A increases from 65% to 80%; the volume percentage of mobile phase B decreases from 35% to 20%;

[0023] When the time period is 85 to 90 minutes: the volume percentage of mobile phase A decreases from 80% to 30%; the volume percentage of mobile phase B increases from 20% to 70%;

[0024] When the time period is 90 to 95 minutes: the volume percentage of mobile phase A is 30%; the volume percentage of mobile phase B is 70%.

[0025] In a possible implementation manner of the first aspect, the preset detection conditions include: a detection wavelength of 254 nm; a flow rate of 1.0 ml / min; and a column temperature of 30°C.

[0026] In a possible implementation of the first aspect, the analyzing the fingerprint of the Yankening tablets and the reference fingerprint to obtain similarity information includes:

[0027] Analyze according to the reference fingerprint spectrum to obtain a key peak characteristic chain;

[0028] Mapping the key peak characteristic chain and the Yankening tablet fingerprint spectrum into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets;

[0029] The similarity information is obtained by analyzing the key peak feature chain and the characteristic peak information of the Yankening tablets.

[0030] In a possible implementation of the first aspect, mapping the key peak characteristic chain and the Yankening tablet fingerprint spectrum into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets includes:

[0031] Mapping the key peak characteristic chain and the Yankening tablet fingerprint spectrum to the same coordinate system, matching and analyzing the chromatographic peaks in the Yankening tablet fingerprint spectrum with the chromatographic peaks reflected by the key peak characteristic chain to obtain a Yankening tablet chromatographic peak analysis group;

[0032] The peak number of the chromatographic peak analysis group of the Yankening Tablets is compared with the peak number of the key peak characteristic chain. If the peak numbers are the same, the chromatographic peak analysis group of the Yankening Tablets is determined as the characteristic peak information of the Yankening Tablets.

[0033] In a possible implementation of the first aspect, mapping the key peak characteristic chain and the Yankening tablet fingerprint spectrum into the same coordinate system for analysis to obtain Yankening tablet characteristic peak information further includes:

[0034] If the number of peaks is different, abnormal peak information is determined; the abnormal peak information is used to reflect the chromatographic peak in the chromatographic peak analysis group of the Yankening Tablets corresponding to the same chromatographic peak in the key peak feature chain;

[0035] Calculating the difference between the retention time of each chromatographic peak in the abnormal peak information and the retention of the chromatographic peak reflected by the key peak feature chain to obtain a difference group;

[0036] The chromatographic peak corresponding to the minimum value in the difference group is retained as the chromatographic peak corresponding to the key peak characteristic chain, and combined with other normal peaks to obtain the characteristic peak information of Yankening Tablets.

[0037] In a possible implementation of the first aspect, matching and analyzing the chromatographic peaks in the fingerprint of the Yankening tablets with the chromatographic peaks reflected by the key peak feature chain to obtain a chromatographic peak analysis group of the Yankening tablets includes:

[0038] Obtaining peak head information and peak tail information of each chromatographic peak in the key peak feature chain; wherein the peak head information is used to reflect the time when the chromatographic peak appears, and the peak tail information is used to reflect the time when the chromatographic peak ends;

[0039] Analyze the peak head information and the peak tail information to obtain a time analysis interval; wherein the time analysis interval is used to reflect the time range of the chromatographic peak in the key peak feature chain;

[0040] The retention time of the chromatographic peak in the fingerprint of Yankening Tablets is matched with the time analysis interval, and the chromatographic peak in the fingerprint of Yankening Tablets within the time analysis interval is screened out to obtain the chromatographic peak analysis group of Yankening Tablets.

[0041] In a second aspect, the present application embodiment provides a Yankening tablet fingerprint detection system, comprising:

[0042] A first analysis module, used to analyze the reference fingerprint spectrum to obtain a key peak feature chain;

[0043] The second analysis module is used to map the key peak characteristic chain and the Yankening tablet fingerprint spectrum into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets;

[0044] The third analysis module is used to analyze the key peak feature chain and the characteristic peak information of the Yankening tablets to obtain similarity information.

[0045] In the third aspect, an embodiment of the present application provides a fingerprint detection device for Yankening tablets, comprising a control device and a high performance liquid chromatograph, wherein the control device is electrically connected to the high performance liquid chromatograph, the control device comprises a memory, a processor, and a computer program stored in the memory and executable on the processor, and when the processor executes the computer program, the method described in any one of the first aspects above is implemented.

[0046] In a fourth aspect, an embodiment of the present application provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the method described in any one of the above-mentioned first aspects is implemented.

[0047] In the fifth aspect, an embodiment of the present application provides a computer program product. When the computer program product is run on a Yankening tablet fingerprint detection device, the Yankening tablet fingerprint detection device enables the Yankening tablet fingerprint detection method described in any one of the first aspects above.

[0048] It can be understood that the beneficial effects of the second to fifth aspects mentioned above can be found in the relevant description of the first aspect mentioned above, and will not be repeated here. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0050] Figure 1 It is a schematic diagram of the process of the fingerprint spectrum detection method of Yankening tablets provided in the embodiment of the present application;

[0051] Figure 2 This is one of the implementation flow diagrams of step S400 in the fingerprint spectrum detection method of Yankening tablets provided in the embodiment of the present application;

[0052] Figure 3 This is the second implementation flow diagram of step S400 in the fingerprint spectrum detection method of Yankening tablets provided in the embodiment of the present application;

[0053] Figure 4 It is a structural schematic diagram of the fingerprint spectrum detection system of Yankening tablets provided in the embodiment of the present application;

[0054] Figure 5 It is a schematic diagram of the structure of the control device of the Yankening tablet fingerprint detection equipment provided in the embodiment of the present application. DETAILED DESCRIPTION

[0055] In the following description, specific details such as specific system structures, technologies, etc. are provided for the purpose of illustration rather than limitation, so as to provide a thorough understanding of the embodiments of the present application. However, it should be clear to those skilled in the art that the present application may also be implemented in other embodiments without these specific details. In other cases, detailed descriptions of well-known systems, devices, circuits, and methods are omitted to prevent unnecessary details from obstructing the description of the present application.

[0056] It should be understood that when used in the present specification and the appended claims, the term "comprising" indicates the presence of described features, wholes, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, wholes, steps, operations, elements, components and / or combinations thereof.

[0057] It should also be understood that the term “and / or” used in the specification and appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0058] As used in the specification of this application and the appended claims, the term "if" can be interpreted as "when" or "uponce" or "in response to determining" or "in response to detecting" depending on the context. Similarly, the phrase "if it is determined" or "if the described condition or event is detected" can be interpreted as meaning "uponce it is determined" or "in response to determining" or "uponce the described condition or event is detected" or "in response to detecting the described condition or event" depending on the context.

[0059] In addition, in the description of the present application specification and the appended claims, the terms "first", "second", "third", etc. are only used to distinguish the descriptions and cannot be understood as indicating or implying relative importance.

[0060] References to "one embodiment" or "some embodiments" etc. described in the specification of this application mean that one or more embodiments of the present application include specific features, structures or characteristics described in conjunction with the embodiment. Therefore, the statements "in one embodiment", "in some embodiments", "in some other embodiments", "in some other embodiments", etc. that appear in different places in this specification do not necessarily refer to the same embodiment, but mean "one or more but not all embodiments", unless otherwise specifically emphasized in other ways. The terms "including", "comprising", "having" and their variations all mean "including but not limited to", unless otherwise specifically emphasized in other ways.

[0061] Yankening tablets are a commonly used Chinese patent medicine, widely used to treat diseases caused by inflammation, such as pharyngitis, tonsillitis, etc. In order to ensure the quality and efficacy of Yankening tablets, it is very necessary to accurately analyze its ingredients qualitatively and quantitatively. In recent years, with the development of science and technology, fingerprint technology has been widely used because it can fully reflect the complexity of Chinese medicine ingredients. Fingerprint technology can qualitatively and quantitatively analyze the various active ingredients in Yankening tablets through high-performance liquid chromatography (HPLC) and other means, thus providing a scientific and accurate means of quality control.

[0062] Traditional fingerprint detection methods for Yankening tablets often rely on the detection of a single component, which is time-consuming and difficult to quickly and comprehensively reflect the complex components and efficacy of Yankening tablets.

[0063] To solve the above problems, the embodiment of the present application provides a fingerprint detection of Yankening tablets. In this method, firstly, a test solution of Yankening tablets and a reference solution are prepared; after the chromatographic conditions are matched with the system suitability, 5 μl of the test solution of Yankening tablets and the reference solution are respectively injected into the liquid chromatograph for measurement to obtain the fingerprint of Yankening tablets and the reference fingerprint; finally, the fingerprint of Yankening tablets and the reference fingerprint are analyzed to obtain similarity information. This method uses a standardized detection process, compares with the reference solution, and detects multiple components in Yankening tablets at the same time, comprehensively reflects the basis of its effective substances, and helps to comprehensively evaluate the quality of Yankening tablets; by comprehensively analyzing the similarity between the fingerprint of Yankening tablets and the reference fingerprint, the quality and consistency of Yankening tablets can be comprehensively evaluated, and the stability and reliability of the quality evaluation of Yankening tablets can be improved.

[0064] The Yankening Tablets fingerprint detection method provided in the embodiment of the present application can be applied to the Yankening Tablets fingerprint detection device. At this time, the Yankening Tablets fingerprint detection device is the execution entity of the Yankening Tablets fingerprint detection method provided in the embodiment of the present application. The embodiment of the present application does not impose any restrictions on the specific type of the Yankening Tablets fingerprint detection device.

[0065] For example, the fingerprint detection equipment of Yankening tablets may include a control device and a high performance liquid chromatograph. The control device may be a mobile phone, a tablet computer, a wearable device, a laptop computer, an ultra-mobile personal computer (UMPC), a netbook, a desktop computer, a smart large screen, a smart TV and other terminal devices, an Internet of Things terminal, a computer, a laptop computer, a handheld communication device, a handheld computing device, etc.

[0066] In order to better understand the fingerprint detection method of Yankening tablets provided in the embodiment of the present application, the specific implementation process of the fingerprint detection method of Yankening tablets provided in the embodiment of the present application is exemplarily introduced below.

[0067] Figure 1 The schematic flow chart of the fingerprint spectrum detection method of Yankening tablets provided in the embodiment of the present application is shown, and the fingerprint spectrum detection method of Yankening tablets includes:

[0068] S100, prepare the test solution of Yankening tablets.

[0069] It can be understood that the test solution of Yankening tablets refers to the analytical solution made of Yankening tablets that need to be quality tested. It can be prepared manually or with automatic preparation equipment, etc., but it is not limited to this. The automatic preparation equipment may include an automatic grinding device, a weighing device, a clamping device, an ultrasonic treatment device, a filtering device and a control device, and the control device is electrically connected to the automatic grinding device, the weighing device, the clamping device, the ultrasonic treatment device, and the filtering device. The automatic grinding device is used to crush and grind the Yankening tablets after removing the coating. The automatic grinding device can be a grinder, a grinding ball, etc., but it is not limited to this. The weighing device is used to weigh the weight of the Yankening powder after grinding and the weight of the solution before and after the subsequent ultrasonic treatment. The clamping device is used to clamp the crushed Yankening tablets and clamp the container containing the solution to move. The clamping device can be a mechanical arm, an electric clamp, etc., but it is not limited to this. The ultrasonic treatment device is used to ultrasonically treat the solution containing the Yankening tablets. The filtering device is used to filter the solution after ultrasonic treatment. The filtering device can be a microporous membrane filter, a fully automatic filter, etc., but is not limited thereto.

[0070] In some embodiments, in step S100, preparing a Yankening tablet test solution comprises:

[0071] S110, take 10 Yankening tablets, remove the coating and grind them into powder, take 0.3g of Yankening powder and put it into a stoppered conical flask.

[0072] It is understood that coating refers to a thin film covering the surface of the tablet, which is used to improve the appearance, taste, stability or control the drug release rate of the tablet. The coating on the surface of the tablet can be carefully scraped off manually with a blade or needle; the tablet can also be immersed in a solvent that can dissolve the coating material to dissolve the coating, etc., but not limited to this. The Yankening tablets after removing the coating can be crushed and ground manually, or ground with an automatic grinder, etc., but not limited to this.

[0073] S120, adding 50 ml of methanol with a purity of 80% into the stoppered conical flask to obtain a first mixed solution, and weighing the mixed solution.

[0074] It can be understood that the weighed weight is the total weight of the yankening powder, methanol and the stoppered conical flask. The weight of the stoppered conical flask can be weighed after the yankening powder and methanol are placed in the stoppered conical flask; the weights of the yankening powder and methanol can be weighed separately before they are placed in the stoppered conical flask, and then the weights are added together, and so on.

[0075] S130, subjecting the first mixed solution to ultrasonic treatment for 30 minutes to obtain a second mixed solution, cooling the second mixed solution to room temperature and weighing the weight, supplementing the weight of the second mixed solution with methanol with a purity of 80%, homogenizing the second mixed solution, filtering, and obtaining a test solution of Yankening tablets; wherein the power of the ultrasonic treatment is 480 W and the frequency is 40 kHz.

[0076] It is understood that ultrasonic treatment is to clean up the impurities and microorganisms in the yankening powder, and the cavitation effect produced by high-frequency vibration destroys the cell wall and tissue structure in the yankening tablet, so that the effective ingredient is more easily dissolved in the solvent. The stoppered conical flask can be naturally cooled to room temperature, or a cooling device (such as a constant temperature cooling box, a water bath, etc.) can be used for cooling. Homogenization includes shaking and centrifugation. Shaking can be done by manually shaking the stoppered conical flask, or the stoppered conical flask can be placed on a vibration table and shaken, etc., but is not limited to this. Centrifugation can be done by manually rotating the stoppered conical flask to separate the solid particles or sediment in the second mixed solution from the liquid portion, or a centrifugal device can be used for centrifugation, etc., but is not limited to this.

[0077] S200, prepare reference solution.

[0078] It is understood that the reference solution is a standard solution for comparative analysis with the Yankening Tablets test solution. It can be prepared by a method similar to the method for preparing the Yankening Tablets test solution in step S100.

[0079] In some embodiments, in step S200, preparing a reference solution comprises:

[0080] Weigh preset weights of berberine hydrochloride reference substance, baicalin reference substance, aloe-emodin reference substance, rhein acid reference substance, rhein reference substance and chrysophanol reference substance, and use 80% pure methanol to make a solution containing 10 μg of berberine hydrochloride, 55 μg of baicalin, 4 μg of aloe-emodin, 10 μg of rhein acid, 2 μg of rhein and 4 μg of chrysophanol per 1 ml to obtain a reference substance solution.

[0081] It can be understood that the preset weight can be weighed according to the different amounts of reference solution used, and there is a one-to-one correspondence between them. The more reference solution is used, the greater the preset weight is, and the more methanol is used.

[0082] S300, after the chromatographic conditions are matched with the system suitability, 5 μl of the test solution and the reference solution of Yankening Tablets are respectively injected into the liquid chromatograph for determination to obtain the fingerprint spectrum of Yankening Tablets and the reference fingerprint spectrum.

[0083] It can be understood that the matching of chromatographic conditions and system suitability refers to the matching of the chromatographic conditions used (such as mobile phase, flow rate, detection wavelength, etc.) and the working state of the liquid chromatography (HPLC) system, so that the analytical method can work normally within the expected range and achieve the expected separation effect and sensitivity.

[0084] In some embodiments, in step S300, the chromatographic conditions are matched with the system suitability, including:

[0085] S310, using octadecylsilane bonded silica gel as filler; using methanol as mobile phase A and 0.2% phosphoric acid solution as mobile phase B, gradient elution is performed; under the preset detection conditions, the theoretical plate number calculated based on the baicalin peak is not less than 2000.

[0086] It can be understood that the calculation formula for the number of theoretical plates is Where t is the retention time of the solute, and W is the width of the chromatographic peak at the baseline. The larger the value, the higher the separation efficiency of the chromatographic column, the sharper the peak shape, and the better the separation effect. Therefore, the minimum limit of the theoretical plate number is specified in the chromatographic analysis method to improve the accuracy and repeatability of the analysis method. The theoretical plate number (N) is a parameter for measuring the separation efficiency of the chromatographic column in chromatographic analysis. It is based on the plate theory and is used to describe the performance of the chromatographic column.

[0087] Optionally, in some embodiments, in step S310, gradient elution is performed, comprising:

[0088] When the time period is 0 to 70 minutes: the volume percentage of mobile phase A increases from 30% to 65%; the volume percentage of mobile phase B decreases from 70% to 35%;

[0089] When the time period is 70 to 85 minutes: the volume percentage of mobile phase A increases from 65% to 80%; the volume percentage of mobile phase B decreases from 35% to 20%;

[0090] When the time period is 85 to 90 minutes: the volume percentage of mobile phase A decreases from 80% to 30%; the volume percentage of mobile phase B increases from 20% to 70%;

[0091] When the time period is 90 to 95 minutes: the volume percentage of mobile phase A is 30%; the volume percentage of mobile phase B is 70%.

[0092] It is understood that gradient elution refers to an elution method that changes the elution capacity by gradually changing the ratio of two or more components in the mobile phase during liquid chromatography analysis. This method can improve the separation effect of each component in a complex mixture, and is particularly suitable for analyzing samples containing multiple compounds. Exemplarily, the gradient program in the following table can be set in the software of the HPLC instrument, and it is ensured that the instrument can automatically adjust the composition of the mobile phase according to the set time period and ratio change, and the flow rate, ratio and elution time of the eluent can also be manually controlled, etc., but not limited to this.

[0093] Table 1 shows the elution program for gradient elution:

[0094] Table 1

[0095]

[0096]

[0097] Optionally, in some embodiments, in step S310, under preset detection conditions, the following steps are included:

[0098] The detection wavelength was 254 nm; the flow rate was 1.0 ml / min; and the column temperature was 30°C.

[0099] It can be understood that setting the flow rate of the mobile phase to 1.0 ml per minute can make the solution move at a moderate speed in the chromatographic column, neither too fast to cause poor separation effect, nor too slow to cause too long analysis time, which helps to improve the resolution of the chromatographic peaks, so that adjacent chromatographic peaks can be better separated, thereby improving the accuracy of the analysis. Setting the column temperature to 30° can provide a relatively stable analysis environment. Temperature has a great influence on chromatographic analysis. The increase in temperature usually increases the distribution coefficient of the sample between the mobile phase and the stationary phase, thereby affecting the retention time. By setting a constant column temperature, the impact of temperature fluctuations on the analysis results can be reduced, and the repeatability and reliability of the data can be improved; in addition, a temperature of 30° usually does not cause thermal damage to the chromatographic column and the sample, which is beneficial to protecting the service life of the chromatographic column and the stability of the sample.

[0100] S400, analyzing the fingerprint spectrum of Yankening tablets and the reference fingerprint spectrum to obtain similarity information.

[0101] It can be understood that similarity information refers to the similarity between the fingerprint of Yankening tablets and the control fingerprint. It can help to judge whether the quality of Yankening tablets meets the standard, so as to ensure the consistency of its quality and efficacy. Exemplarily, each chromatographic peak in the fingerprint of Yankening tablets can be extracted, and then the degree of coincidence or overlap between each chromatographic peak and the control fingerprint can be analyzed to obtain similarity information; it is also possible to directly compare the control fingerprint with the fingerprint of Yankening tablets, such as inputting it into the learning model to obtain similarity information, etc., but it is not limited thereto. The learning model is trained by multiple sets of training data, and each set of training data in the multiple sets of training data includes the fingerprint of Yankening tablets to be tested and the control fingerprint and similarity of the standard.

[0102] In one possible implementation, see Figure 2 and Figure 3 In step S400, the fingerprint of Yankening tablets and the reference fingerprint are analyzed to obtain similarity information, including:

[0103] S410, analyzing according to the reference fingerprint spectrum to obtain a key peak feature chain.

[0104] It can be understood that since the reference solution corresponding to the reference fingerprint is made of berberine hydrochloride reference, baicalin reference, aloe-emodin reference, rhein reference, rhein reference and chrysophanol reference with specific content and concentration, the key peak feature chain is used to reflect the retention time and content of each reference, that is, the time point of the chromatographic peak and the total area under the peak. For example, the key peak feature chain is obtained by using chromatography software (such as Agilent ChemStation, Waters Empower, etc.) for peak identification, or by manually marking each chromatographic peak in the reference spectrum and recording each feature of the chromatographic peak.

[0105] S420, mapping the key peak characteristic chain and the fingerprint spectrum of Yankening tablets into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets.

[0106] It can be understood that the control fingerprint and the Yankening tablets fingerprint can be used respectively to mark and record the characteristics of each chromatographic peak, including retention time, peak area, peak height, etc., and the contours of these characteristics can be converted into coordinate points, creating a new coordinate system, setting the horizontal axis as retention time and the vertical axis as signal intensity (such as peak height or peak area), and importing all converted coordinate points into the coordinate system.

[0107] Exemplarily, the fingerprint of Yankening Tablets can be matched with the key peak characteristic chain to obtain the chromatographic peak group corresponding to the key peak characteristic chain in the fingerprint of Yankening Tablets, and then the chromatographic peak group can be further analyzed and screened to obtain the characteristic peak information of Yankening Tablets; each chromatographic peak of the fingerprint of Yankening Tablets can also be matched with the chromatographic peak reflected by the key peak characteristic chain, and the chromatographic peak of the fingerprint of Yankening Tablets corresponding to the coordinate can be marked to obtain the characteristic peak of Yankening Tablets, and so on, but not limited to this.

[0108] In one possible implementation, see Figure 3 In step S420, the key peak feature chain and the Yankening tablet fingerprint spectrum are mapped to the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets, including:

[0109] S421, mapping the key peak characteristic chain and the fingerprint spectrum of Yankening Tablets to the same coordinate system, matching and analyzing the chromatographic peaks in the fingerprint spectrum of Yankening Tablets with the chromatographic peaks reflected by the key peak characteristic chain, and obtaining the chromatographic peak analysis group of Yankening Tablets.

[0110] It is understandable that Yankening Tablets may be affected by other substances, which may introduce new chromatographic peaks or change the response values ​​of known components. Therefore, the chromatographic peaks in the fingerprint of Yankening Tablets are often more than the number of chromatographic peaks reflected by the key peak characteristic chain. In addition, there may be a situation where two chromatographic peaks in the fingerprint of Yankening Tablets are close to the same chromatographic peak in the key peak characteristic chain. Therefore, the chromatographic peak analysis group of Yankening Tablets may not be completely one-to-one corresponding to the key peak characteristic chain.

[0111] Exemplarily, the retention time and existence time of the chromatographic peak can be used as matching features to match the chromatographic peak in the fingerprint of Yankening Tablets with the chromatographic peak reflected by the key peak feature chain to obtain the chromatographic peak analysis group of Yankening Tablets, or the chromatographic peak whose retention time difference between the retention time of the chromatographic peak in the fingerprint of Yankening Tablets and the chromatographic peak reflected by the key peak feature chain within the preset difference range can be used as the chromatographic peak analysis group of Yankening Tablets, etc., but not limited to this. The preset difference range is a pre-set range value, which can be manually input by humans, or can be obtained in the detection database, etc., but not limited to this. The data in the detection database can be obtained by means such as laboratory experiments, on-site measurements and monitoring, and previous experience. After obtaining, the collected data is sorted, classified and archived, useful information and rules are extracted, and the relevant data are saved in the database to form a detection database.

[0112] In one possible implementation, see Figure 3In step S421, the chromatographic peaks in the fingerprint of Yankening tablets are matched and analyzed with the chromatographic peaks reflected by the key peak feature chain to obtain the chromatographic peak analysis group of Yankening tablets, including:

[0113] S4211, obtaining the peak head information and peak tail information of each chromatographic peak in the key peak feature chain; wherein the peak head information is used to reflect the time when the chromatographic peak appears, and the peak tail information is used to reflect the time when the chromatographic peak ends.

[0114] Exemplarily, each chromatographic peak in the key peak feature chain can be directly located in the coordinates to obtain the peak head information and the peak tail information.

[0115] S4212, analyzing the peak head information and the peak tail information to obtain a time analysis interval; wherein the time analysis interval is used to reflect the time range of the chromatographic peak in the key peak feature chain.

[0116] It can be understood that the time of the peak head and the peak tail of each chromatographic peak is calculated to obtain the time analysis interval. For example, the peak head information of chromatographic peak No. 1 of the key peak feature chain is 21.875min, and the peak tail information is 22.056min, then the time analysis interval is (21.875~22.056), and so on.

[0117] S4213, matching the retention time of the chromatographic peak in the fingerprint of Yankening tablets with the time analysis interval, screening out the chromatographic peak in the fingerprint of Yankening tablets within the time analysis interval, and obtaining the chromatographic peak analysis group of Yankening tablets.

[0118] For example, assuming that the time analysis intervals corresponding to the chromatographic peaks in the key peak feature chain are (21.875-22.056), (32.523-33.102) and (60.658-61.956), if the retention times of all chromatographic peaks in the fingerprint of Yankening Tablets are 10.542, 16.568, 18.654, 21.985, 32.879, 54.369, 61.326, 82.542, respectively, then the chromatographic peak analysis group of Yankening Tablets contains It includes the chromatographic peaks corresponding to 21.985, 32.879, and 61.326; if the retention times of all chromatographic peaks in the fingerprint of Yankening Tablets are 10.542, 16.568, 18.654, 21.985, 22.012, 32.879, 54.369, 61.326, and 82.542, respectively, then the chromatographic peak analysis group of Yankening Tablets includes the chromatographic peaks corresponding to 21.985, 22.012, 32.879, 61.326, and so on.

[0119] With this setting, by obtaining the peak head information and peak tail information of each chromatographic peak, the time range of the chromatographic peak can be determined more accurately, thereby improving the matching accuracy; in the screening process, interfering peaks that are not within the time analysis interval can be excluded, thereby reducing the possibility of misjudgment.

[0120] S422A, compare the peak number of the chromatographic peak analysis group of Yankening Tablets with the peak number of the key peak characteristic chain. If the peak numbers are the same, the chromatographic peak analysis group of Yankening Tablets is determined as the characteristic peak information of Yankening Tablets.

[0121] It can be understood that, assuming that the time analysis intervals corresponding to the chromatographic peaks in the key peak characteristic chain are (21.875-22.056), (32.523-33.102) and (60.658-61.956), respectively, if the retention times of all chromatographic peaks in the fingerprint of Yankening Tablets are 10.542, 16.568, 18.654, 21.985, 32.879, 54.369, 61.326 and 82.542, respectively, then the chromatographic peak analysis group of Yankening Tablets includes the chromatographic peaks corresponding to 21.985, 32.879 and 61.326. At this time, the number of peaks in the chromatographic peak analysis group of Yankening Tablets is the same as the number of peaks in the key peak characteristic chain, then the chromatographic peaks corresponding to 21.985, 32.879 and 61.326 are determined as the characteristic peak information of Yankening Tablets.

[0122] In one possible implementation, see Figure 3 In step S400, the key peak feature chain and the fingerprint spectrum of Yankening tablets are mapped to the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets, and further comprising:

[0123] S422a, if the number of peaks is different, determine the abnormal peak information; the abnormal peak information is used to reflect the chromatographic peak of the same chromatographic peak in the corresponding key peak feature chain in the chromatographic peak analysis group of Yankening Tablets.

[0124] It can be understood that if the retention times of all chromatographic peaks in the fingerprint of Yankening Tablets are 10.542, 16.568, 18.654, 21.985, 22.012, 32.879, 54.369, 61.326, and 82.542 respectively, then the chromatographic peak analysis group of Yankening Tablets includes the chromatographic peaks corresponding to 21.985, 22.012, 32.879, and 61.326. At this time, the number of peaks in the chromatographic peak analysis group of Yankening Tablets is different from the number of peaks in the key peak characteristic chain, and the abnormal peak information is the chromatographic peaks corresponding to the retention times of 21.985 and 22.012.

[0125] S423, calculating the difference between the retention time of each chromatographic peak in the abnormal peak information and the retention of the chromatographic peak reflected by the key peak feature chain to obtain a difference group.

[0126] It can be understood that, assuming that the time analysis interval in the key peak feature chain is (21.875-22.056), the retention time corresponding to the chromatographic peak is 21.951, the retention time corresponding to the chromatographic peak (32.523-33.102) is 32.879, the retention time corresponding to the chromatographic peak (60.658-61.956) is 61.326, and the abnormal peak information is the chromatographic peaks corresponding to the retention times of 21.985 and 22.012, then the difference between 21.985 and 21.951 is calculated to be 0.034, the difference between 22.012 and 21.951 is 0.061, and the difference group is a set of 0.034 and 0.061.

[0127] S424, retaining the chromatographic peak corresponding to the minimum value in the difference group as the chromatographic peak corresponding to the key peak characteristic chain, and combining it with other normal peaks to obtain the characteristic peak information of Yankening Tablets.

[0128] It can be understood that, according to step S423, assuming that the difference group obtained is 0.034 and 0.061, the chromatographic peak corresponding to 0.034 is marked and combined with other chromatographic peaks without abnormalities to obtain the characteristic peak information of Yankening Tablets.

[0129] With this setting, during the screening process, interfering peaks with large differences in retention time from the key peak characteristic chain can be excluded, thereby reducing the possibility of misjudgment. By accurately calculating the retention time difference, it can be ensured that the closest matching peak can be found even if there is a small deviation, thereby improving the matching accuracy. The chromatographic peaks that meet the conditions can be quickly screened out, saving a lot of manual comparison time.

[0130] S430, analyzing the key peak feature chain and the characteristic peak information of Yankening Tablets to obtain similarity information.

[0131] Exemplarily, the key peak feature chain and the characteristic peak information of Yankening Tablets can be analyzed by the peak matching method, that is, by comparing the characteristic peak information of Yankening Tablets with the peak position, peak area, peak height and other features of the key peak feature chain, and evaluating the similarity by calculating the peak position deviation, area deviation, etc.; the evaluation can also be performed by comprehensively considering multiple features (such as peak position, peak area, peak height, etc.), that is, extracting the deviation or overlap of the key features of each peak (such as peak position, peak area, peak height, etc.), assigning weights to each feature, and then calculating the weighted score, summarizing the weighted scores, and obtaining a comprehensive similarity score, etc., but not limited to this.

[0132] With this setting, by comparing the key peak feature chain with the fingerprint spectrum of Yankening Tablets in the same coordinate system, it can be ensured that the characteristics such as retention time, peak area and peak height are compared under the same standard, thereby improving the accuracy of matching. At the same time, the chromatographic peaks to be analyzed can be clearly identified, interfering peaks can be effectively excluded, and the possibility of misjudgment can be reduced.

[0133] It should be understood that the size of the serial numbers of the steps in the above embodiments does not mean the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0134] Corresponding to the Yankening Tablets fingerprint detection method described in the above embodiment, the embodiment of the present application also provides a Yankening Tablets fingerprint detection system, and the various modules of the system can implement the various steps of the Yankening Tablets fingerprint detection method. Figure 4 A structural block diagram of the fingerprint detection system of Yankening tablets provided in an embodiment of the present application is shown. For the sake of ease of explanation, only the parts related to the embodiment of the present application are shown.

[0135] Reference Figure 4 The Yankening tablets fingerprint detection system includes:

[0136] In a second aspect, the present application embodiment provides a Yankening tablet fingerprint detection system, comprising:

[0137] The first analysis module is used to analyze according to the reference fingerprint spectrum to obtain the key peak feature chain.

[0138] The second analysis module is used to map the key peak feature chain and the fingerprint spectrum of Yankening Tablets into the same coordinate system for analysis to obtain the characteristic peak information of Yankening Tablets.

[0139] The third analysis module is used to analyze the key peak feature chain and the characteristic peak information of Yankening Tablets to obtain similarity information.

[0140] It should be noted that the information interaction, execution process and other contents between the above-mentioned modules are based on the same concept as the method embodiment of the present application. Their specific functions and technical effects can be found in the method embodiment part and will not be repeated here.

[0141] Those skilled in the art can clearly understand that, for the convenience and simplicity of description, only the division of the above modules is used as an example for illustration. In practical applications, the above functions can be assigned to different modules as needed, that is, the internal structure of the system can be divided into different modules to complete all or part of the functions described above. The modules in the embodiment can be integrated into a processing unit, or each module can exist physically alone, or two or more modules can be integrated into one unit. The above integrated unit can be implemented in the form of hardware or in the form of software functional units. In addition, the specific names of the modules are only for the convenience of distinguishing from each other, and are not used to limit the scope of protection of this application. The specific working process of the modules in the above system can refer to the corresponding process in the aforementioned method embodiment, which will not be repeated here.

[0142] The present application also provides a fingerprint detection device for Yankening tablets, including a control device and a high performance liquid chromatograph, wherein the control device is electrically connected to the high performance liquid chromatograph. The control device 6 includes: at least one processor 60 ( Figure 5 Only one is shown), at least one memory 61 ( Figure 5 Only one is shown) and a computer program 62 stored in the at least one memory 61 and executable on the at least one processor 60. When the processor 60 executes the computer program 62, the control device 6 implements the steps in any of the above-mentioned embodiments of the fingerprint spectrum detection method of Yankening tablets, or implements the functions of the modules in the above-mentioned embodiments.

[0143] Exemplarily, the computer program 62 may be divided into one or more modules / units, which are stored in the memory 61 and executed by the processor 60 to complete the present application. The one or more modules / units may be a series of computer program instruction segments capable of completing specific functions, which are used to describe the execution process of the computer program 62 in the control device 6.

[0144] The control device 6 may be a computing device such as a desktop computer, a notebook, a PDA, or a cloud server. The control device may include, but is not limited to, a processor 60 and a memory 61. Those skilled in the art will appreciate that Figure 5 It is only an example of the control device 6 and does not constitute a limitation on the control device 6. It may include more or fewer components than shown in the figure, or a combination of certain components, or different components. For example, it may also include input and output devices, network access devices, buses, etc.

[0145] The processor 60 may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor or any conventional processor, etc.

[0146] In some embodiments, the memory 61 may be an internal storage unit of the control device 6, such as a hard disk or memory of the control device 6. In other embodiments, the memory 61 may also be an external storage device of the control device 6, such as a plug-in hard disk, a smart media card (SMC), a secure digital (SD) card, a flash card, etc. equipped on the control device 6. Further, the memory 61 may also include both an internal storage unit and an external storage device of the control device 6. The memory 61 is used to store an operating system, an application program, a boot loader (BootLoader), data, and other programs, such as the program code of the computer program. The memory 61 may also be used to temporarily store data that has been output or is to be output.

[0147] An embodiment of the present application further provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the steps in any of the above method embodiments are implemented.

[0148] An embodiment of the present application provides a computer program product. When the computer program product runs on a Yankening tablet fingerprint detection device, the Yankening tablet fingerprint detection device implements the steps in any of the above-mentioned method embodiments.

[0149] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the present application implements all or part of the process in the above-mentioned embodiment method, which can be completed by instructing the relevant hardware through a computer program, and the computer program can be stored in a computer-readable storage medium, and the computer program can be executed by the processor. The steps of each method embodiment can be implemented. Wherein, the computer program includes a computer program code, and the computer program code can be in source code form, object code form, executable file or some intermediate form. The computer-readable medium may at least include: any entity or device, recording medium, computer memory, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), electric carrier signal, telecommunication signal and software distribution medium that can carry the computer program code to the fingerprint spectrum detection device of Yankening tablets. For example, a USB flash drive, a mobile hard disk, a disk or an optical disk.

[0150] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described or recorded in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0151] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0152] In the embodiments provided in the present application, it should be understood that the disclosed Yankening Tablets fingerprint spectrum detection device and system can be implemented in other ways. For example, the above-described Yankening Tablets fingerprint spectrum detection system embodiment is only schematic. For example, the division of the modules is only a logical function division. There may be other division methods in actual implementation. For example, multiple modules can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interfaces, devices or modules, which can be electrical, mechanical or other forms.

[0153] The modules described as separate components may or may not be physically separated, and the components shown as modules may or may not be physical modules, that is, they may be located in one place or distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0154] The embodiments described above are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, a person skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application, and should all be included in the protection scope of the present application.

Claims

1. A fingerprint detection method for Yankening tablets, characterized in that: include: Prepare Yankening tablets test solution; Prepare reference solution; After the chromatographic conditions are matched with the system suitability, 5 μl of the Yankening Tablets test solution and the reference solution are respectively injected into the liquid chromatograph for determination to obtain the Yankening Tablets fingerprint spectrum and the reference fingerprint spectrum; The fingerprint spectrum of the Yankening tablets and the reference fingerprint spectrum are analyzed to obtain similarity information.

2. The fingerprint detection method of Yankening tablets according to claim 1, characterized in that: The method for preparing the Yankening tablet test solution comprises: Take 10 Yankening tablets, remove the coating and grind into powder, take 0.3g Yankening powder and put it into a stoppered conical bottle; Then, add 50 ml of 80% pure methanol into the stoppered conical flask to obtain a first mixed solution, and weigh the mixture; The first mixed liquid is ultrasonically treated for 30 minutes to obtain a second mixed liquid. The second mixed liquid is cooled to room temperature and then weighed. The weight of the second mixed liquid is supplemented with methanol with a purity of 80%, and then the second mixed liquid is homogenized and filtered to obtain a test solution of Yankening tablets; wherein the power of the ultrasonic treatment is 480 W and the frequency is 40 kHz.

3. The fingerprint detection method of Yankening tablets according to claim 1, characterized in that: The preparation of the reference substance solution comprises: Weigh preset weights of berberine hydrochloride reference substance, baicalin reference substance, aloe-emodin reference substance, rhein acid reference substance, rhein reference substance and chrysophanol reference substance, and use 80% pure methanol to make a solution containing 10 μg of berberine hydrochloride, 55 μg of baicalin, 4 μg of aloe-emodin, 10 μg of rhein acid, 2 μg of rhein and 4 μg of chrysophanol per 1 ml to obtain a reference substance solution.

4. The fingerprint detection method of Yankening tablets according to claim 1, characterized in that: The chromatographic conditions were matched to the system suitability and included: Octadecylsilane bonded silica gel is used as filler; methanol is used as mobile phase A and 0.2% phosphoric acid solution is used as mobile phase B for gradient elution; under preset detection conditions, the theoretical plate number is not less than 2000 calculated based on the baicalin peak.

5. The fingerprint detection method of Yankening tablets according to claim 4, characterized in that: The gradient elution comprises: When the time period is 0 to 70 minutes: the volume percentage of mobile phase A increases from 30% to 65%; the volume percentage of mobile phase B decreases from 70% to 35%; When the time period is 70 to 85 minutes: the volume percentage of mobile phase A increases from 65% to 80%; the volume percentage of mobile phase B decreases from 35% to 20%; When the time period is 85 to 90 minutes: the volume percentage of mobile phase A decreases from 80% to 30%; the volume percentage of mobile phase B increases from 20% to 70%; When the time period is 90 to 95 minutes: the volume percentage of mobile phase A is 30%; the volume percentage of mobile phase B is 70%.

6. The fingerprint detection method of Yankening tablets according to claim 4, characterized in that: The preset detection conditions include: a detection wavelength of 254 nm; a flow rate of 1.0 ml / min; and a column temperature of 30°C.

7. The fingerprint detection method of Yankening tablets according to claim 1, characterized in that: The similarity information is obtained by analyzing the fingerprint spectrum of the Yankening tablets and the reference fingerprint spectrum, including: Analyze according to the reference fingerprint spectrum to obtain a key peak characteristic chain; Mapping the key peak characteristic chain and the Yankening tablet fingerprint spectrum into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets; The similarity information is obtained by analyzing the key peak feature chain and the characteristic peak information of the Yankening tablets.

8. The fingerprint detection method of Yankening tablets according to claim 7, characterized in that: The key peak characteristic chain and the Yankening tablet fingerprint spectrum are mapped into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets, including: Mapping the key peak characteristic chain and the Yankening tablet fingerprint spectrum to the same coordinate system, matching and analyzing the chromatographic peaks in the Yankening tablet fingerprint spectrum with the chromatographic peaks reflected by the key peak characteristic chain to obtain a Yankening tablet chromatographic peak analysis group; The peak number of the chromatographic peak analysis group of the Yankening Tablets is compared with the peak number of the key peak characteristic chain. If the peak numbers are the same, the chromatographic peak analysis group of the Yankening Tablets is determined as the characteristic peak information of the Yankening Tablets.

9. The fingerprint detection method of Yankening tablets according to claim 8, characterized in that: The key peak characteristic chain and the Yankening tablet fingerprint spectrum are mapped into the same coordinate system for analysis to obtain the characteristic peak information of Yankening tablets, and further includes: If the number of peaks is different, abnormal peak information is determined; the abnormal peak information is used to reflect the chromatographic peak in the chromatographic peak analysis group of the Yankening Tablets corresponding to the same chromatographic peak in the key peak feature chain; Calculating the difference between the retention time of each chromatographic peak in the abnormal peak information and the retention of the chromatographic peak reflected by the key peak feature chain to obtain a difference group; The chromatographic peak corresponding to the minimum value in the difference group is retained as the chromatographic peak corresponding to the key peak characteristic chain, and combined with other normal peaks to obtain the characteristic peak information of Yankening Tablets.

10. The fingerprint detection method of Yankening tablets according to claim 8, characterized in that: The chromatographic peaks in the fingerprint of the Yankening tablets are matched and analyzed with the chromatographic peaks reflected by the key peak characteristic chain to obtain a chromatographic peak analysis group of the Yankening tablets, including: Obtaining peak head information and peak tail information of each chromatographic peak in the key peak feature chain; wherein the peak head information is used to reflect the time when the chromatographic peak appears, and the peak tail information is used to reflect the time when the chromatographic peak ends; Analyze the peak head information and the peak tail information to obtain a time analysis interval; wherein the time analysis interval is used to reflect the time range of the chromatographic peak in the key peak feature chain; The retention time of the chromatographic peak in the fingerprint of Yankening Tablets is matched with the time analysis interval, and the chromatographic peak in the fingerprint of Yankening Tablets within the time analysis interval is screened out to obtain the chromatographic peak analysis group of Yankening Tablets.

Images