Skin-care composition for relieving allergy and repairing as well as preparation method and application of skin-care composition

Abstract

The invention relates to an anti-allergy and repairing skin care composition and a preparation method and application thereof.The anti-allergy and repairing skin care composition comprises an oil-phase raw material, a water-phase raw material and a surfactant which are emulsified, the oil-phase raw material comprises hydroxytyrosol, olive oil and kojic acid dipalmitate, and the water-phase raw material comprises hydroxytyrosol, olive oil and kojic acid dipalmitate; the water-phase raw materials comprise a cyclodextrin-included eurotium cristatum fermentation extract, Ectoine and water. According to the skin care composition disclosed by the invention, the eurotium cristatum fermentation extract is included by the cyclodextrin, so that the stability, the solubility and the bioavailability of the eurotium cristatum fermentation extract are improved, and the irritation is reduced; according to the skin care composition, the hydroxytyrosol, the olive oil, the kojic acid dipalmitate, the cyclodextrin-included eurotium cristatum fermentation extract and the Ectoine are compounded, and all the components have a synergistic effect in the aspect of improving the allergy-relieving and repairing effects of the product.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and relates to a soothing and repairing skin care composition and a preparation method and application thereof. Background Art

[0002] Allergic reaction refers to a series of overreactions made by the immune system when the human body comes into contact with specific allergens (such as insect venom, food and drugs, injections through skin contact, etc.). This phenomenon is considered to be a disorder of the immune system. It can also be said that allergic reaction is an abnormal, pathological, and harmful immune reaction to the human body, also known as hypersensitivity or allergic reaction. When cosmetics or skin care products are applied to the surface of human skin, abnormal immune reactions may occur to specific chemicals in them, causing disorders in the body's physiological functions, and even the risk of tissue damage, triggering a series of clinical symptoms that may be mild or severe. Skin allergic reactions usually cause dilation of capillaries on the surface of the skin, increased cell permeability, tissue degeneration or even necrosis, as well as symptoms such as desquamation, swelling, redness, itching, and dryness on the surface of the skin.

[0003] Eurotium cristatum, also known as golden flower, is a strain that plays an important role in the fermentation process of tea. The secondary metabolites produced by it have extremely strong biological activity, covering multiple categories such as benzaldehyde derivatives, anthraquinone derivatives, diketopiperazine indole alkaloids, polyketides, benzoic acids, phenols, polysaccharides, etc. The secondary metabolites of Eurotium cristatum have anti-inflammatory and soothing and repairing effects, but the secondary metabolite ferulic acid produced by it is unstable in nature and is an easily oxidized substance, which will make the color of cosmetics yellow and smell bad. In addition, the carboxyl and hydroxyl groups of ferulic acid are easy to hydrate, but the benzene ring and the side chain containing double bonds have high hydrophobicity, which makes ferulic acid poorly water-soluble and fat-soluble. Under light conditions, the double bonds in the side chain of ferulic acid are prone to flipping, causing trans-cis photoisomerization, which makes ferulic acid unstable to light. The impact of ferulic acid on the formula and low bioavailability limit the application of secondary metabolites produced by Eurotium cristatum containing ferulic acid in cosmetics.

[0004] Therefore, how to develop excellent formulation technology containing secondary metabolites of Eriobotrya coronaria, inhibit the degradation and isomerization of ferulic acid, reduce the impact of ferulic acid on the cosmetic formula system, and prevent the color and smell of cosmetics from changing are still urgent problems to be solved. Summary of the invention

[0005] In view of the deficiencies in the prior art, the object of the present invention is to provide a soothing and repairing skin care composition and a preparation method and application thereof.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In the first aspect, the present invention provides a soothing and repairing skin care composition, which comprises a composition formed by emulsification of oil phase raw materials, water phase raw materials and surfactants, wherein the oil phase raw materials comprise hydroxytyrosol, olive oil and kojic acid dipalmitate, and the water phase raw materials comprise cyclodextrin-encapsulated Eurotium cristolon fermentation extract, ectoine and water.

[0008] The skin care composition of the present invention includes the fermented extract of Eurotium cristatum with cyclodextrin, thereby improving the stability, solubility and bioavailability of the fermented extract of Eurotium cristatum and reducing irritation; hydroxytyrosol not only has the effect of soothing and repairing, but also can protect the ferulic acid in the fermented extract from being oxidized, improve the stability of ferulic acid, and prevent the product from turning yellow and smelling bad; olive oil has antioxidant and anti-inflammatory activities, can help the skin resist the damage of free radicals, reduce inflammatory reactions, and contribute to the repair and regeneration of skin cells, and olive oil has good permeability and skin affinity The property is used to enhance the transdermal absorption of the fermented extract of Eurotium cristatum, and further improve the soothing and repairing performance of the product; kojic acid dipalmitate can inhibit the activity of aminotransferase, regulate the skin's inflammatory response, and has a certain soothing effect on sensitive skin and acne skin. When used in combination with the fermented extract of Eurotium cristatum, it can protect the skin from oxidative stress damage, thereby contributing to soothing and repairing and delaying skin aging; ectoine can reduce the degradation or photoisomerization reaction of ferulic acid under light conditions through its protective effect, thereby improving the photostability of ferulic acid, thereby further improving the soothing and repairing performance of the product. Therefore, the skin care composition involved in the present invention is compounded by five components: hydroxytyrosol, olive oil, kojic acid dipalmitate, cyclodextrin-encapsulated Eurotium cristatum fermented extract, and ectoine, and each component synergizes in improving the soothing and repairing effect of the product.

[0009] The present invention also utilizes the solubilization and stabilization effects of the nanoemulsion to further improve the solubility and stability of the skin care composition. At the same time, the nanoemulsion has a smaller particle size and can more easily penetrate the stratum corneum of the skin, thereby enhancing the transdermal absorption of the skin care composition, solving the problem of poor transdermal absorption of the cyclodextrin-encapsulated Eurotium cristatum fermentation extract.

[0010] Preferably, the raw materials for preparing the soothing and repairing skin care composition include, by mass, 15-21 parts of hydroxytyrosol, 2-10 parts of olive oil, 12-24 parts of kojic acid dipalmitate, 25-30 parts of cyclodextrin, 25-35 parts of Eurotium cristatum fermentation extract, 25-30 parts of ectoine, 10-50 parts of water, and 10-25 parts of surfactant.

[0011] When the raw materials for preparing the soothing and repairing skin care composition are formulated in the above-mentioned specific mass ratio, it has a more significant effect in improving the soothing and repairing efficacy of the product.

[0012] The mass fraction of the hydroxytyrosol can be selected from 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, etc.; the mass fraction of the olive oil can be selected from 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the kojic acid dipalmitate can be selected from 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, etc.; the mass fraction of the cyclodextrin can be selected from 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, etc.; the mass fraction of the fermented extract of the cosmidia cristatum can be selected from 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, etc. The mass fraction of the described surfactant can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 24 parts, 25 parts, etc.; the mass fraction of the described ectoine can be selected from 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, etc.; the mass fraction of the described water can be selected from 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, etc.; the mass fraction of the described surfactant can be selected from 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 24 parts, 25 parts, etc.; other specific point values ​​within the above numerical ranges can be selected, and they will not be described one by one here.

[0013] Preferably, the oil phase raw material also includes almond oil.

[0014] Almond oil can cover up the unpleasant smell of oxidized ferulic acid and has good skin penetration properties. It cooperates synergistically with olive oil in enhancing the transdermal absorption of the fermented extract of Eurotium cristatum and improving the product's soothing and repairing properties.

[0015] Preferably, the raw materials for preparing the soothing and repairing skin care composition also include 2-10 parts of almond oil by mass, for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc. Other specific points within this numerical range can be selected and will not be described here one by one.

[0016] Preferably, the surfactant includes any one of PEG-6 caprylic / capric glyceride, PEG-8 caprylic / capric glyceride, Tween 80, Tween 20, polyoxyethylene castor oil, Span 80, glyceryl monostearate, lecithin, stearyl alcohol polyether or polyethylene glycol stearate, or a combination of at least two thereof.

[0017] Preferably, the raw materials for preparing the soothing and repairing skin care composition also include 10-20 parts by mass of a co-surfactant, for example, 10 parts, 12 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc.; the co-surfactant is selected from any one of diethylene glycol monoethyl ether, dipropylene glycol, 1,3-butanediol, ethanol, 1,2-pentanediol, 1,2-hexanediol, propylene glycol, glycerol or polyethylene glycol, or a combination of at least two of them.

[0018] Preferably, the fermentation extract of Eurotium cristatum is prepared by a preparation method comprising the following steps:

[0019] (1) crushing black tea, green tea, golden camellia and oat bran and mixing them with water to obtain a fermentation substrate solution;

[0020] (2) mixing a bacterial solution of Eurotium cristatum, cellulase and a fermentation substrate solution, fermenting and sterilizing to obtain a fermentation product;

[0021] (3) crushing the fermentation product, and then mixing it with an ethanol aqueous solution to perform diafiltration extraction to obtain an extract;

[0022] (4) filtering the extract to obtain the fermentation extract of Eurotium cristatum.

[0023] The fermented extract of Eurotium cristatum used in the present invention is preferably prepared by the above-mentioned specific preparation process, selecting a combination of black tea, green tea, golden camellia and oat bran as the fermentation substrate, inoculating the bacterial liquid of Eurotium cristatum and cellulase into the fermentation substrate for co-fermentation, and finally obtaining the fermented extract of Eurotium cristatum by crushing treatment combined with ethanol percolation extraction. The product prepared by this process has a better soothing and repairing effect than the products prepared by other processes.

[0024] Preferably, the fermentation substrate solution comprises, by mass, 12-24 parts of dark tea, 5-10 parts of green tea, 5-10 parts of golden camellia, 5-10 parts of oat bran, and 50-120 parts of water.

[0025] Preferably, the fermentation substrate solution further comprises 5-10 parts by mass of citric acid, which can promote the dissolution of active ingredients in the aforementioned fermentation substrate in water.

[0026] The mass fraction of the dark tea can be selected from 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts, etc.; the mass fraction of the green tea can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the golden camellia can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the oat bran can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the water can be selected from 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, 100 parts, 110 parts, 120 parts, etc.; the mass fraction of citric acid can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; other specific point values ​​within the numerical range of the above items can be selected, and they will not be repeated here one by one.

[0027] Preferably, the crushing is to 60-100 mesh, for example, 60 mesh, 65 mesh, 70 mesh, 75 mesh, 80 mesh, 85 mesh, 90 mesh, 95 mesh, 100 mesh, etc.

[0028] Preferably, the viable bacterial count of the Eurotium cristatum bacterial solution is (5-9)×10 9 CFU / mL, for example 5×10 9 CFU / mL, 6×10 9 CFU / mL, 7×10 9 CFU / mL, 8×10 9 CFU / mL, 9×10 9 CFU / mL, etc.

[0029] Preferably, the inoculation amount of the Eurotium cristatum bacterial solution is 1-3%, for example, 1%, 1.5%, 2%, 2.5%, 3%, etc.

[0030] Preferably, the cellulase is mixed in the form of a solution with a concentration of 1-5 U / mL, such as 1 U / mL, 2 U / mL, 3 U / mL, 4 U / mL, 5 U / mL, etc.; and its usage is 0.2-2% of the fermentation substrate solution, such as 0.2%, 0.5%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc.

[0031] Preferably, the fermentation temperature is 10-45°C, for example, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, etc.; the fermentation time is 20-90h, for example, 20h, 30h, 40h, 50h, 60h, 70h, 80h, 90h, etc.

[0032] Preferably, the sterilization is carried out at 70-90°C (e.g., 70°C, 75°C, 80°C, 85°C, 90°C, etc.) for 10-20 min (e.g., 10 min, 13 min, 16 min, 18 min, 20 min, etc.).

[0033] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0034] Preferably, the crushing treatment is high-pressure homogenization at 50-70 MPa (e.g., 50 MPa, 55 MPa, 60 MPa, 65 MPa, 70 MPa, etc.) for 5-15 min (e.g., 5 min, 8 min, 9 min, 10 min, 12 min, 13 min, 15 min, etc.).

[0035] Preferably, the volume fraction of the ethanol aqueous solution is 60-80%, for example 60%, 65%, 70%, 75%, 80%, etc.; the mass is 5-10 times that of the fermentation product, for example 5 times, 6 times, 7 times, 8 times, 9 times, 10 times, etc.

[0036] Preferably, the percolation extraction is carried out at 30-60°C (e.g., 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, etc.) for 3-8h (e.g., 3h, 4h, 5h, 6h, 7h, 8h, etc.).

[0037] Preferably, the filtration is followed by concentration and drying.

[0038] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0039] In the present invention, the cyclodextrin-encapsulated Eurotium cristatum fermentation extract is prepared by a preparation method comprising the following steps:

[0040] Dissolving cyclodextrin in water to obtain a cyclodextrin solution; mixing the cyclodextrin solution with the fermentation extract of Eurotium cristatum, stirring, centrifuging, precipitating and drying to obtain the cyclodextrin-encapsulated fermentation extract of Eurotium cristatum.

[0041] Preferably, the temperature of the water is 50-60°C, for example, 50°C, 52°C, 55°C, 56°C, 57°C, 58°C, 59°C, 60°C, etc.

[0042] Preferably, the stirring is carried out at 20-50°C (e.g., 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, etc.) for 20-60min (e.g., 20min, 25min, 30min, 35min, 40min, 45min, 50min, 55min, 60min, etc.).

[0043] Preferably, the centrifugation is performed at 1800-2200 rpm (e.g., 1800 rpm, 1900 rpm, 2000 rpm, 2100 rpm, 2200 rpm, etc.) for 20-30 min (e.g., 20 min, 22 min, 24 min, 26 min, 28 min, 30 min, etc.).

[0044] Preferably, after drying, the sieve is further sieved through a 100-150 mesh sieve, such as a 100 mesh sieve, a 110 mesh sieve, a 120 mesh sieve, a 130 mesh sieve, a 140 mesh sieve, a 150 mesh sieve, etc.

[0045] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0046] In a second aspect, the present invention provides a method for preparing the soothing and repairing skin care composition according to the first aspect, the preparation method comprising:

[0047] mixing an optional co-surfactant with kojic acid dipalmitate, olive oil, optional almond oil, then with a surfactant, and finally with hydroxytyrosol to obtain an oil phase;

[0048] Mixing various raw materials of the water phase to obtain a water phase;

[0049] The oil phase and the water phase are mixed and emulsified to obtain the soothing and repairing skin care composition.

[0050] In a third aspect, the present invention provides use of the soothing and repairing skin care composition described in the first aspect in the preparation of cosmetics.

[0051] Compared with the prior art, the present invention has the following beneficial effects:

[0052] The skin care composition involved in the present invention includes the fermented extract of Eurotium cristatum with cyclodextrin, thereby improving the stability, solubility and bioavailability of the fermented extract of Eurotium cristatum and reducing the irritation; the skin care composition involved in the present invention compoundes five components including hydroxytyrosol, olive oil, kojic acid dipalmitate, cyclodextrin-included fermented extract of Eurotium cristatum and ectoine, and the components synergistically enhance the soothing and repairing effect of the product. DETAILED DESCRIPTION

[0053] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0054] The Eurotium cristatum involved in the following preparation examples is a product with model number Bio-59287 purchased from Beijing Biobowei Biotechnology Co., Ltd.; the cellulase is a product with model number YB37524 purchased from Hunan Yunbang Biotechnology Co., Ltd.; and the cyclodextrin is a product with model number α-cyclodextrin purchased from Shandong Tongwang Biotechnology Co., Ltd.

[0055] Preparation Example 1

[0056] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method is as follows:

[0057] (1) 18 parts of dark tea, 7 parts of green tea, 7 parts of golden camellia and 8 parts of oat bran are crushed and passed through an 80-mesh sieve, and mixed with 90 parts of water and 7 parts of citric acid to obtain a fermentation substrate solution;

[0058] (2) Add the fungus solution (8×10 9 CFU / mL) at an inoculation amount of 2% and a cellulase solution (3U / mL) at a usage amount of 1% were mixed with the fermentation substrate solution of step (1), fermented at 36°C for 72h, and sterilized at 90°C for 15min to obtain a fermentation product;

[0059] (3) The fermentation product was homogenized under high pressure at 60 MPa for 10 min, and then mixed with 8 times the mass of 70% ethanol aqueous solution and subjected to diafiltration extraction at 50° C. for 5 h to obtain an extract;

[0060] (4) filtering the extract, concentrating and drying to obtain the fermentation extract of Eurotium cristatum.

[0061] A cyclodextrin-encapsulated fermented extract of Eurotium cristatum is also provided, and the preparation method is as follows:

[0062] Dissolve 25 parts of cyclodextrin in 60°C water to prepare a saturated cyclodextrin solution; mix the cyclodextrin solution with 25 parts of the above-prepared fermentation extract of Eurotium cristatum, stir at 45°C for 50 minutes, centrifuge at 2000 rpm for 30 minutes, precipitate and dry to obtain the product.

[0063] Preparation Example 2

[0064] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method is as follows:

[0065] (1) crushing 12 parts of dark tea, 10 parts of green tea, 10 parts of golden camellia and 5 parts of oat bran, passing through a 100-mesh sieve, and mixing with 120 parts of water and 10 parts of citric acid to obtain a fermentation substrate solution;

[0066] (2) Add the fungus solution (9×10 9CFU / mL) at an inoculation amount of 1% and a cellulase solution (1U / mL) at a usage amount of 2% are mixed with the fermentation substrate solution of step (1), fermented at 40°C for 60h, and sterilized at 80°C for 20min to obtain a fermentation product;

[0067] (3) The fermentation product was homogenized under high pressure at 70 MPa for 5 min, and then mixed with 10 times the mass of 60% ethanol aqueous solution and subjected to diafiltration extraction at 40° C. for 8 h to obtain an extract;

[0068] (4) filtering the extract, concentrating and drying to obtain the fermentation extract of Eurotium cristatum.

[0069] A cyclodextrin-encapsulated fermented extract of Eurotium cristatum is also provided, and the preparation method is as follows:

[0070] Dissolve 30 parts of cyclodextrin in 50°C water to prepare a saturated cyclodextrin solution; mix the cyclodextrin solution with 35 parts of the fermentation extract of Eurotium cristatum prepared above, stir at 40°C for 60 minutes, centrifuge at 2200 rpm for 20 minutes, precipitate and dry to obtain the product.

[0071] Preparation Example 3

[0072] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method is as follows:

[0073] (1) crushing 24 parts of dark tea, 5 parts of green tea, 5 parts of golden camellia and 10 parts of oat bran, passing through a 60-mesh sieve, and mixing with 60 parts of water and 5 parts of citric acid to obtain a fermentation substrate solution;

[0074] (2) Add the bacterial solution of Eurotium cristatum (5×10 9 CFU / mL) at an inoculation amount of 2% and a cellulase solution (3U / mL) at a usage amount of 0.5% were mixed with the fermentation substrate solution of step (1), fermented at 35°C for 90h, and sterilized at 90°C for 20min to obtain a fermentation product;

[0075] (3) The fermentation product was homogenized under high pressure at 50 MPa for 15 min, and then mixed with 5 times the mass of 80% ethanol aqueous solution and subjected to diafiltration extraction at 60° C. for 4 h to obtain an extract;

[0076] (4) filtering the extract, concentrating and drying to obtain the fermentation extract of Eurotium cristatum.

[0077] A cyclodextrin-encapsulated fermented extract of Eurotium cristatum is also provided, and the preparation method is as follows:

[0078] Dissolve 27.5 parts of cyclodextrin in 50°C water to prepare a saturated cyclodextrin solution; mix the cyclodextrin solution with 30 parts of the above-prepared fermentation extract of Eurotium cristatum, stir at 50°C for 40 minutes, centrifuge at 1800 rpm for 30 minutes, precipitate and dry to obtain the product.

[0079] Preparation Example 4

[0080] This preparation example provides a fermented extract of Eurotium cristatum, and the preparation method thereof is different from that of Preparation Example 1 only in that: dark tea is lacking in the fermentation substrate, and its reduced mass is proportionally distributed to the mass of green tea, golden camellia and oat bran, and other conditions remain unchanged.

[0081] Also provided is a cyclodextrin-encapsulated fermentation extract of Eurotium cristatum, the preparation method of which is the same as that of Preparation Example 1.

[0082] Preparation Example 5

[0083] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method thereof is different from that of Preparation Example 1 only in that green tea is lacking in the fermentation substrate, and its reduced mass is proportionally distributed to the mass of black tea, golden camellia and oat bran, while other conditions remain unchanged.

[0084] Also provided is a cyclodextrin-encapsulated fermentation extract of Eurotium cristatum, the preparation method of which is the same as that of Preparation Example 1.

[0085] Preparation Example 6

[0086] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method thereof is different from that of Preparation Example 1 only in that: the fermentation substrate lacks Camellia chrysantha, and its reduced mass is proportionally distributed to the mass of black tea, green tea and oat bran, and other conditions remain unchanged.

[0087] Also provided is a cyclodextrin-encapsulated fermentation extract of Eurotium cristatum, the preparation method of which is the same as that of Preparation Example 1.

[0088] Preparation Example 7

[0089] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method thereof is different from that of Preparation Example 1 only in that oat bran is lacking in the fermentation substrate, and its reduced mass is proportionally distributed to the mass of black tea, green tea and golden camellia, while other conditions remain unchanged.

[0090] Also provided is a cyclodextrin-encapsulated fermentation extract of Eurotium cristatum, the preparation method of which is the same as that of Preparation Example 1.

[0091] Preparation Example 8

[0092] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method thereof is different from that of Preparation Example 1 only in that in step (2), no cellulase solution is added to mix with the fermentation substrate solution, and other conditions remain unchanged.

[0093] Also provided is a cyclodextrin-encapsulated fermentation extract of Eurotium cristatum, the preparation method of which is the same as that of Preparation Example 1.

[0094] Preparation Example 9

[0095] This preparation example provides a fermentation extract of Eurotium cristatum, and the preparation method thereof is different from that of Preparation Example 1 only in that: in step (1), citric acid is not added to the fermentation substrate, and other conditions remain unchanged.

[0096] Also provided is a cyclodextrin-encapsulated fermentation extract of Eurotium cristatum, the preparation method of which is the same as that of Preparation Example 1.

[0097] The olive oil involved in the following embodiments and comparative examples is a product purchased from Jiangxi Yisenyuan Plant Flavor Co., Ltd. under the model of olive oil; the almond oil is a product purchased from Jiangxi Yisenyuan Plant Flavor Co., Ltd. under the model of sweet almond oil; and the polyoxyethylene castor oil is a product purchased from Wuhan Lanabai Pharmaceutical Chemical Co., Ltd. under the model of polyoxyethylene castor oil.

[0098] Example 1

[0099] The present embodiment provides a soothing and repairing skin care composition, wherein the raw materials for its preparation include, by weight: 21 parts of hydroxytyrosol, 5 parts of olive oil, 24 parts of kojic acid dipalmitate, 50 parts of the cyclodextrin-encapsulated fermented extract of Eurotium cristatum obtained in Preparation Example 1, 25 parts of ectoine, 2 parts of PEG-6 caprylic / capric glyceride, 6 parts of polyoxyethylene castor oil, 8 parts of 1,3-butylene glycol, 10 parts of glycerol, and 20 parts of water;

[0100] The preparation method is as follows:

[0101] 1,3-Butanediol, glycerin, kojic acid dipalmitate and olive oil were mixed and stirred at 65°C for 20 minutes; PEG-6 caprylic / capric glyceride and polyoxyethylene castor oil were then added and stirred for 20 minutes; hydroxytyrosol was then added and stirred for 20 minutes to obtain an oil phase;

[0102] Mix water, the cyclodextrin-encapsulated Eurotium cristatum fermentation extract prepared in Preparation Example 1, and ectoin, and stir at 45° C. for 15 minutes to obtain an aqueous phase;

[0103] The oil phase and the water phase are mixed, and emulsified and homogenized at 45°C to obtain a primary emulsion;

[0104] The primary emulsion is secondarily emulsified using a high-pressure homogenizing emulsifier to obtain a soothing and repairing skin care composition.

[0105] Example 2

[0106] The present embodiment provides a soothing and repairing skin care composition, wherein the raw materials for its preparation include, by weight: 15 parts of hydroxytyrosol, 10 parts of olive oil, 12 parts of kojic acid dipalmitate, 65 parts of the cyclodextrin-encapsulated fermented extract of Eurotium cristatum obtained in Preparation Example 1, 30 parts of ectoine, 2 parts of PEG-8 caprylic / capric glyceride, 6 parts of polyoxyethylene castor oil, 6 parts of dipropylene glycol, 6 parts of glycerin, and 30 parts of water;

[0107] The preparation method is as follows:

[0108] Dipropylene glycol, glycerin, kojic acid dipalmitate and olive oil were mixed and stirred at 60°C for 30 minutes; PEG-8 caprylic / capric glyceride and polyoxyethylene castor oil were then added and stirred for 30 minutes; hydroxytyrosol was then added and stirred for 20 minutes to obtain an oil phase;

[0109] Mix water, the cyclodextrin-encapsulated Eurotium cristatum fermentation extract prepared in Preparation Example 1, and ectoin, and stir at 40° C. for 20 minutes to obtain an aqueous phase;

[0110] The oil phase and the water phase are mixed, and emulsified and homogenized at 40°C to obtain a primary emulsion;

[0111] The primary emulsion is secondarily emulsified using a high-pressure homogenizing emulsifier to obtain a soothing and repairing skin care composition.

[0112] Example 3

[0113] The present embodiment provides a soothing and repairing skin care composition, wherein the raw materials for its preparation include, by weight: 18 parts of hydroxytyrosol, 6 parts of olive oil, 18 parts of kojic acid dipalmitate, 57.5 parts of the cyclodextrin-encapsulated fermented extract of Eurotium cristatum obtained in Preparation Example 1, 27.5 parts of ectoine, 2 parts of PEG-8 caprylic / capric glyceride, 7 parts of polyoxyethylene castor oil, 6 parts of 1,2-pentanediol, 9 parts of glycerol, and 25 parts of water;

[0114] The preparation method is as follows:

[0115] Mix 1,2-pentanediol, glycerin, kojic acid dipalmitate and olive oil, and stir at 60°C for 30 minutes; then add PEG-8 caprylic / capric glyceride and polyoxyethylene castor oil, and continue stirring for 25 minutes; then add hydroxytyrosol, and continue stirring for 20 minutes to obtain an oil phase;

[0116] Mix water, the cyclodextrin-encapsulated Eurotium cristatum fermentation extract prepared in Preparation Example 1, and ectoin, and stir at 40° C. for 20 minutes to obtain an aqueous phase;

[0117] The oil phase and the water phase are mixed, and emulsified and homogenized at 40°C to obtain a primary emulsion;

[0118] The primary emulsion is secondarily emulsified using a high-pressure homogenizing emulsifier to obtain a soothing and repairing skin care composition.

[0119] Embodiment 4-9

[0120] This embodiment provides six skin care compositions for soothing and repairing skin. The difference between the raw materials and those in Example 1 is that the cyclodextrin-encapsulated fermented extract of Eurotium cristatum prepared in Preparation Example 1 is replaced by the cyclodextrin-encapsulated fermented extract of Eurotium cristatum prepared in Preparation Examples 4-9 in equal amounts, and the other components and contents remain unchanged. The preparation method is the same as in Example 1.

[0121] Example 10

[0122] This embodiment provides a soothing and repairing skin care composition, the raw materials of which are different from those in Example 1 in that 5 parts of olive oil are replaced by 3 parts of olive oil and 2 parts of almond oil, and the other components and contents remain unchanged. The preparation method is the same as in Example 1.

[0123] Comparative Example 1

[0124] This comparative example provides a skin care composition for soothing and repairing. The raw materials for its preparation differ from those in Example 1 only in that hydroxytyrosol is lacking, and its reduced mass is proportionally distributed to the mass of cyclodextrin-encapsulated Eurotium cristatum fermented extract, kojic acid dipalmitate, olive oil, and ectoin, while other components and contents remain unchanged. The preparation method is referred to Example 1.

[0125] Comparative Example 2

[0126] This comparative example provides a soothing and repairing skin care composition, the raw materials of which are different from those of Example 1 only in that kojic acid dipalmitate is missing, and its reduced mass is proportionally distributed to the mass of cyclodextrin-encapsulated Eurotium cristatum fermentation extract, hydroxytyrosol, olive oil, and ectoine, while other components and contents remain unchanged. The preparation method is referred to Example 1.

[0127] Comparative Example 3

[0128] This comparative example provides a skin care composition for soothing and repairing. The raw materials for its preparation differ from those in Example 1 only in that olive oil is missing, and its reduced mass is proportionally distributed to the mass of cyclodextrin-encapsulated Eurotium cristatum fermented extract, hydroxytyrosol, kojic acid dipalmitate, and ectoin, while other components and contents remain unchanged. The preparation method is referred to Example 1.

[0129] Comparative Example 4

[0130] This comparative example provides a soothing and repairing skin care composition, the raw materials of which differ from those of Example 1 only in that ectoine is missing, and its reduced mass is proportionally distributed to the mass of cyclodextrin-encapsulated Eurotium cristatum fermentation extract, hydroxytyrosol, olive oil, and kojic acid dipalmitate, while other components and contents remain unchanged. The preparation method is referred to Example 1.

[0131] Comparative Example 5

[0132] This comparative example provides a skin care composition for soothing and repairing. The raw materials for its preparation differ from those in Example 1 only in that the fermented extract of Eurotium cristatum encapsulated by cyclodextrin is missing, and its reduced mass is proportionally distributed to the mass of ectoine, hydroxytyrosol, olive oil, and kojic acid dipalmitate, while the other components and their contents remain unchanged. The preparation method is referred to Example 1.

[0133] Comparative Example 6

[0134] This comparative example provides a skin care composition for soothing and repairing skin. The raw materials for its preparation differ from those in Example 1 only in that hydroxytyrosol is missing, and its reduced mass is allocated to the mass of ectoine, while other components and contents remain unchanged. The preparation method is the same as in Example 1.

[0135] Comparative Example 7

[0136] This comparative example provides a skin care composition for soothing and repairing skin. The raw materials for its preparation differ from those in Example 1 only in that it lacks ectoine, and its reduced mass is distributed to the mass of hydroxytyrosol, while other components and contents remain unchanged. The preparation method is the same as in Example 1.

[0137] Test Example 1

[0138] Safety performance evaluation:

[0139] The safety performance of the products of Examples 1-10 and Comparative Examples 1-7 was evaluated. 50 social volunteers who met the requirements, aged 20-45 years old, were selected and randomly divided into 5 groups, and each group tried 3 or 4 different products. Placed in the small chamber of the spot tester, the control well was not treated in any way. The spot tester with the test substance was applied to the inner side of the forearm of the subject with a low-allergenic tape, and gently pressed with the palm of the hand to evenly apply it to the skin for 24 hours. Observe the skin reaction according to the standards in Table 1 at 30 minutes, 24 hours and 48 hours after removing the test substance spot tester.

[0140] Table 1

[0141]

[0142]

[0143] The results show that the products of Examples 1-10 of the present invention and Comparative Examples 1-7 all had negative reactions after trial use, indicating that the skin care composition of the present invention is safe and mild and will not cause adverse reactions such as skin irritation and sensitization.

[0144] Test Example 2

[0145] Evaluation of hyaluronidase inhibition effect:

[0146] The hyaluronidase inhibition effect of the products of Examples 1-10 and Comparative Examples 1-7 was evaluated. Hyaluronic acid is a component in the tissue matrix that limits the diffusion of water and other extracellular substances. After being hydrolyzed by hyaluronidase, the cells become non-viscous, causing cell degranulation and exudation of new synthesized media, exerting biological effects, and leading to the occurrence of immediate allergic reactions. Hyaluronidase is a participant in allergic reactions and has a strong correlation with allergies. Allergic phenomena can be alleviated and skin can be improved by inhibiting the activity of hyaluronidase.

[0147] Take 0.1mL CaCl2 solution (0.25mmol / L) and 0.5mL hyaluronidase solution (100U / mL) were reacted in a 37°C water bath for 20min; 0.5mL of a 5-fold dilution of the product obtained in each embodiment and comparative example was added and kept warm for 20min; 0.5mL sodium hyaluronate solution (0.5g / L) was added, and after reacting for 30min, the mixture was taken out and cooled for 5min; 0.1mL sodium hydroxide solution (0.4mol / L) and 0.5mL acetylacetone solution (3.5mL acetylacetone was dissolved in 50mL of 1.0mol / L sodium carbonate solution) were added, and the mixture was immediately transferred to an ice water bath for 5min after boiling in a water bath for 15min; 1mL Ehrlich reagent (0.8g p-dimethylaminobenzaldehyde was dissolved in 15mL concentrated hydrochloric acid and 15mL anhydrous ethanol) was added dropwise, and diluted with 3mL anhydrous ethanol, and placed at 25°C for 20min for color development, and its absorbance was measured at 540nm, and the hyaluronidase inhibition rate was calculated according to the following formula:

[0148] Hyaluronidase inhibition rate = {[(AB)-(CD)] / (AB)}×100%

[0149] In the formula: A is the absorbance of the control solution (using acetate buffer solution instead of the sample solution); B is the absorbance of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C is the absorbance of the sample solution; D is the absorbance of the sample blank solution (using acetate buffer solution instead of the enzyme solution).

[0150] The results are shown in Table 2:

[0151] Table 2

[0152] Sample name Inhibition rate of hyaluronidase (%) Example 1 91.5 Example 2 89.3 Example 3 90.8 Example 4 86.1 Example 5 85.8 Example 6 86.3 Example 7 87.5 Example 8 83.7 Example 9 88.2 Example 10 90.2 Comparative Example 1 76.6 Comparative Example 2 71.9 Comparative Example 3 81.5 Comparative Example 4 80.1 Comparative Example 5 69.6 Comparative Example 6 72.7 Comparative Example 7 82.4

[0153] It can be seen from the data in Table 2 that the skin care composition involved in the present invention has a good inhibitory effect on hyaluronidase, with an inhibition rate of more than 83.7%, and therefore has a soothing effect on the skin. By comparing the data results of Example 1 and Comparative Examples 1-5, it can be seen that the five components of hydroxytyrosol, olive oil, kojic acid dipalmitate, cyclodextrin-encapsulated Eurotium cristatum fermentation extract, and ectoine can mutually promote the performance of the above-mentioned effects of the skin care composition, verifying the rationality of the component combination. By comparing the data results of Example 1 and Examples 4-9, it can be seen that the preparation process of the Eurotium cristatum fermentation extract also affects the performance of the above-mentioned effects to a certain extent.

[0154] Test Example 3

[0155] Evaluation of the effect of soothing skin redness:

[0156] The products of Examples 1-10 and Comparative Examples 1-7 were evaluated for their effect of soothing skin redness. 170 social volunteers who met the requirements were selected, aged 20-55 years old, regardless of gender. They were randomly divided into 17 groups, and each group tried 10 times diluted products of Examples 1-10 and Comparative Examples 1-7. Filter paper soaked with 50 μL of 10% lactic acid solution was applied to the nasolabial grooves on both sides of the volunteers at the same time. When the stimulation score reached ≥2 points (stimulation end point), the filter paper was removed. Different test samples were applied to the test areas on both sides at the same time, with a sample amount of 0.1 mL in each area. The VISIA image analysis system was used to collect images of the facial skin of the subjects, and the a* value of the skin before and 30 minutes after the sample was used was tested with Media Cybernetics Image-Pro Plus 7.0. The a* value is an indicator of skin redness. The larger the a* value, the more serious the skin redness. A* value change rate (%) = (data after use - data before use) / data before use × 100%. The results are shown in Table 3.

[0157] Table 3

[0158]

[0159]

[0160] From the data in Table 3, it can be seen that the skin care composition of the present invention has an excellent effect in soothing skin redness. By comparing the data results of Example 1 and Comparative Examples 1-5, it can be seen that the five components of hydroxytyrosol, olive oil, kojic acid dipalmitate, cyclodextrin-encapsulated Eurotium cristatum fermented extract, and ectoin can mutually promote the performance of the above-mentioned effects of the skin care composition, verifying the rationality of the component combination. At the same time, by comparing the data results of Example 1 and Examples 4-9, it can be seen that the preparation process of the Eurotium cristatum fermented extract also affects the performance of the above-mentioned effects to a certain extent.

[0161] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0162] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0163] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A soothing and repairing skin care composition, characterized in that: The soothing and repairing skin care composition includes a composition formed by emulsifying oil phase raw materials, water phase raw materials and surfactants, wherein the oil phase raw materials include hydroxytyrosol, olive oil and kojic acid dipalmitate, and the water phase raw materials include cyclodextrin-encapsulated Eurotium cristatum fermentation extract, ectoine and water.

2. The soothing and repairing skin care composition according to claim 1, characterized in that: The preparation raw materials of the soothing and repairing skin care composition include, by weight: 15-21 parts of hydroxytyrosol, 2-10 parts of olive oil, 12-24 parts of kojic acid dipalmitate, 25-30 parts of cyclodextrin, 25-35 parts of Eurotium cristatum fermentation extract, 25-30 parts of ectoine, 10-50 parts of water, and 10-25 parts of surfactant; Preferably, the oil phase raw material further comprises almond oil; Preferably, the raw materials for preparing the soothing and repairing skin care composition further include 2-10 parts of almond oil by weight.

3. The soothing and repairing skin care composition according to claim 1 or 2, characterized in that: The surfactant includes any one or a combination of at least two of PEG-6 caprylic / capric glyceride, PEG-8 caprylic / capric glyceride, Tween 80, Tween 20, polyoxyethylene castor oil, Span 80, glyceryl monostearate, lecithin, stearyl alcohol polyether or polyethylene glycol stearate; Preferably, the raw materials for preparing the soothing and repairing skin care composition also include 10-20 parts by mass of a co-surfactant, and the co-surfactant is selected from any one of diethylene glycol monoethyl ether, dipropylene glycol, 1,3-butylene glycol, ethanol, 1,2-pentanediol, 1,2-hexanediol, propylene glycol, glycerol or polyethylene glycol, or a combination of at least two of them.

4. The soothing and repairing skin care composition according to any one of claims 1 to 3, characterized in that: The fermented extract of Eurotium cristatum is prepared by a preparation method comprising the following steps: (1) crushing black tea, green tea, golden camellia and oat bran and mixing them with water to obtain a fermentation substrate solution; (2) mixing a bacterial solution of Eurotium cristatum, cellulase and a fermentation substrate solution, fermenting and sterilizing to obtain a fermentation product; (3) crushing the fermentation product, and then mixing it with an ethanol aqueous solution to perform diafiltration extraction to obtain an extract; (4) filtering the extract to obtain the fermentation extract of Eurotium cristatum.

5. The soothing and repairing skin care composition according to claim 4, characterized in that: The fermentation substrate solution comprises, by weight, 12-24 parts of dark tea, 5-10 parts of green tea, 5-10 parts of golden camellia, 5-10 parts of oat bran, and 50-120 parts of water; Preferably, the fermentation substrate solution further comprises 5-10 parts by mass of citric acid; Preferably, the pulverization is to 60-100 mesh.

6. The soothing and repairing skin care composition according to claim 4 or 5, characterized in that: The viable bacterial count of the cristatum cristatum bacterial liquid is (5-9)×10 9 CFU / mL; Preferably, the inoculation amount of the Eurotium cristatum bacterial solution is 1-3%; Preferably, the cellulase is mixed in the form of a solution, the concentration of which is 1-5 U / mL, and the amount used is 0.2-2% of the fermentation substrate solution; Preferably, the fermentation temperature is 10-45°C and the fermentation time is 20-90h; Preferably, the sterilization is carried out at 70-90°C for 10-20 min.

7. The soothing and repairing skin care composition according to any one of claims 4 to 6, characterized in that: The crushing process is high pressure homogenization at 50-70 MPa for 5-15 minutes; Preferably, the volume fraction of the ethanol aqueous solution is 60-80%, and the mass is 5-10 times that of the fermentation product; Preferably, the percolation extraction is carried out at 30-60° C. for 3-8 h; Preferably, the filtration is followed by concentration and drying.

8. The soothing and repairing skin care composition according to any one of claims 1 to 7, characterized in that: The cyclodextrin-enclosed Eurotium cristatum fermentation extract is prepared by a preparation method comprising the following steps: Dissolving cyclodextrin in water to obtain a cyclodextrin solution; mixing the cyclodextrin solution with the fermentation extract of Eurotium cristatum, stirring, centrifuging, precipitating and drying to obtain the cyclodextrin-encapsulated fermentation extract of Eurotium cristatum; Preferably, the temperature of the water is 50-60°C; Preferably, the stirring is performed at 20-50° C. for 20-60 min; Preferably, the centrifugation is performed at 1800-2200 rpm for 20-30 min; Preferably, the drying is followed by sieving through a 100-150 mesh sieve.

9. The method for preparing the soothing and repairing skin care composition according to any one of claims 1 to 8, characterized in that: The preparation method comprises: mixing an optional co-surfactant with kojic acid dipalmitate, olive oil, optional almond oil, then with a surfactant, and finally with hydroxytyrosol to obtain an oil phase; Mixing various raw materials of the water phase to obtain a water phase; The oil phase and the water phase are mixed and emulsified to obtain the soothing and repairing skin care composition.

10. Use of the soothing and repairing skin care composition according to any one of claims 1 to 8 in the preparation of cosmetics.

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