Traditional Chinese medicine composition for preventing and treating ischemic heart failure as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention relates to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for preventing and treating ischemic heart failure, which is prepared from the following raw material medicines in parts by weight: 3-30 parts of edible tulip, 3-30 parts of herba artemisiae anomalae, 10-40 parts of astragalus membranaceus, 10-30 parts of codonopsis pilosula, 3-30 parts of honeysuckle and 3-20 parts of lily. The invention further discloses a preparation method of the traditional Chinese medicine preparation for preventing and treating the ischemic heart failure and application of the traditional Chinese medicine composition in preventing and treating the ischemic heart failure by inhibiting myocarditis reaction. The controllability and stability of single use of the traditional Chinese medicine medicament are improved, the storage life of the medicament is prolonged, the characteristics and advantages of the traditional Chinese medicine are exerted, the clinical requirements for preventing and treating ischemic heart failure are met, the use difficulty of the traditional Chinese medicine medicament is reduced, and popularization and use are facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine combination for preventing and treating ischemic heart failure, and a preparation method and application thereof. Background Art

[0002] In recent years, ischemic heart failure (IHF) caused by myocardial infarction has become a major disease threatening human health. The number of deaths related to cardiovascular diseases has increased year by year worldwide, bringing a heavy burden to the global economy and society.

[0003] At present, the clinical progress of chemical drugs for the treatment of IHF is relatively slow. For decades, they have mainly focused on traditional drugs such as beta-blockers, ACEI / ARB, aldosterone receptor antagonists, and diuretics. However, the incidence and mortality of IHF remain high and continue to rise without a turning point. Possible reasons include the limited effects of these drugs themselves, and the side effects of the drugs also limit their clinical application. More and more evidence shows that traditional Chinese medicine has advantages in treating IHF. It can not only improve the clinical symptoms of patients and alleviate the side effects of Western medicine, but also improve the cardiac function and quality of life of IHF patients.

[0004] For example, Chinese patent document CN102423347B discloses a Chinese medicine composition for treating heart failure and its application: 10-15 parts of light aconite root, 20-40 parts of raw astragalus, 15-20 parts of antler slices, 20-30 parts of scutellaria baicalensis, and 10-15 parts of raw white peony root. This drug combination is mainly used to improve clinical symptoms and ischemic symptoms caused by palpitations. However, the above-mentioned Chinese medicine composition needs to be decocted for a long time when used, which is inconvenient for patients to use under hospitalization conditions, and the degree of promotion of use is reduced. If it is decocted in advance, since they are all water extracts, the active ingredients therein are easily affected and difficult to be used for a long time. At the same time, because the water decoction technology is difficult to completely release the active ingredients in the medicinal materials, the efficacy of a single portion of Chinese medicine is not stable, which further causes the situation that it is difficult to see the effect and difficult to promote.

[0005] Therefore, there is a need for a Chinese medicine combination and Chinese medicine preparation that is easy to use and has stable efficacy to increase the promotion of Chinese medicine drugs for preventing and treating ischemic heart failure. Summary of the invention

[0006] The purpose of the present invention is to provide a Chinese medicine combination for preventing and treating ischemic heart failure and a preparation method and application thereof, so as to solve the technical problems in the prior art.

[0007] In order to solve the above technical problems, the present invention specifically provides the following technical solutions:

[0008] The invention provides a Chinese medicine combination for preventing and treating ischemic heart failure. The Chinese medicine composition is prepared from the following raw materials in parts by weight: 3-30 parts of Trillium gracile, 3-30 parts of Rhizoma Cynomorii, 10-40 parts of Radix Astragali, 10-30 parts of Codonopsis pilosula, 3-30 parts of Flos Lonicerae and 3-20 parts of Lilium. The Chinese medicine composition is the raw material of a Chinese medicine preparation for immediate prevention and treatment of ischemic heart failure.

[0009] The invention provides a traditional Chinese medicine preparation for preventing and treating ischemic heart failure. The traditional Chinese medicine preparation contains water extracts of Tripterygium wilfordii, Astragalus membranaceus, Codonopsis pilosula, Honeysuckle and Lilium odoratum.

[0010] The present invention provides a method for preparing a Chinese medicine preparation for preventing and treating ischemic heart failure, comprising the following steps:

[0011] S100, freeze-drying the Psoralea corylifolia to obtain freeze-dried Psoralea corylifolia blocks, and grinding the freeze-dried blocks at low temperatures to obtain Psoralea corylifolia fine powder;

[0012] S200, boiling a mixture of Rhizoma Cynanchum, Radix Astragali, Radix Codonopsis, Flos Lonicerae, and Bulbus Lilii with water for multiple times, combining the filtrates, and concentrating the filtrates under reduced pressure to obtain a medicinal solution;

[0013] S300, adding the Psoralea corylifolia powder into the medicinal solution to prepare the traditional Chinese medicine preparation.

[0014] As a preferred embodiment of the present invention, in S200, the mass fraction of the water is 6-10 times that of the traditional Chinese medicine composition.

[0015] As a preferred embodiment of the present invention, in S200, the decocting times is 2 to 3 times.

[0016] As a preferred embodiment of the present invention, in S200, the boiling time for each time is 40 to 60 minutes.

[0017] As a preferred embodiment of the present invention, in S200, each milliliter of the medicinal solution contains 0.2 g to 3 g of the crude drug.

[0018] The present invention provides an application of a Chinese medicine combination in preventing and treating ischemic heart failure. The Chinese medicine combination prevents and treats ischemic heart failure by inhibiting myocardial inflammatory response.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention freeze-dries the Trillium pilosula, thereby grinding it into fine powder at low temperature, improving the uniformity of the Trillium pilosula solid particles in the medicinal solution, facilitating stable production, improving the controllability and stability of single-use Chinese medicines, and giving full play to the characteristics and advantages of traditional Chinese medicine to prevent and treat ischemic heart failure, which has clinical needs;

[0021] The present invention preserves the effective ingredients in solid particles, retains the effective ingredients in Tripterygium wilfordii to the greatest extent, increases the shelf life of the anti-inflammatory ingredients, and facilitates long-term storage and immediate use;

[0022] The invention decocts, crushes or compresses Chinese medicinal materials respectively to obtain instant medicines that can be packaged and preserved, thereby reducing the difficulty of using Chinese medicinal medicines and further improving the efficacy according to the medicinal properties, thereby facilitating popularization and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0024] Figure 1 The present invention provides a schematic flow chart of a Chinese medicine preparation for preventing and treating ischemic heart failure;

[0025] Figure 2 The present invention provides a comparison chart of the effects of the Yiqi Zhuyu Huadu prescription on myocardial infarction area in Example 1. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Improving IHF by inhibiting inflammatory response is an innovative direction, but there are few patents with clear evidence of innovative Chinese medicine combinations, preparation methods and applications for improving IHF by inhibiting inflammatory response.

[0028] In order to improve IHF from the perspective of anti-inflammatory, the present invention provides a Chinese medicine combination for preventing and treating ischemic heart failure, and a preparation method and application thereof, including Trillium gracile, Ligusticum wallichii, Astragalus membranaceus, Codonopsis pilosula, Honeysuckle and Lilium bulb.

[0029] Chinese medicine composition of the present invention can be used for ischemic heart failure (including ischemic heart disease and heart failure patients after cardiac surgery). Chinese medicine believes that the pathogenesis of ischemic heart failure is mainly based on deficiency of the root and excess of the side, and the basic pathogenesis is chest yang deficiency, yin evil ascending yang position, yin evil blocking, and chest and back qi stagnation and obstruction. The main manifestation of deficiency of the root is qi and yang deficiency, which causes insufficient heart qi and yang deficiency, and qi and yang deficiency can gradually develop into qi deficiency and inability to circulate blood, qi does not transform body fluid, yang deficiency cold coagulation, phlegm stasis tissue qi movement, etc., which affects the normal operation of the heart and falls ill, and blood stasis and poison are mutually causal and mutually transformed, and poisonous evil invades the heart and causes the disharmony of body fluid, qi and blood, and visceral dysfunction causes angina, i.e. "no evil is not toxic, heat is transformed from poison, change is caused by poison, and blood stasis is caused by poison". The inventor found that Chinese medicine composition of the present invention can significantly improve the cardiac function of ischemic heart failure animal model, reduce myocardial infarction area, and inhibit ischemic heart failure cardiac inflammatory reaction through a large number of drug screenings, thereby preventing and treating ischemic heart failure.

[0030] In the prescription of the present invention, the monarch medicine is Rhizoma Cibotii and Rhizoma Ligustici. Rhizoma Cibotii is sweet, slightly pungent, cool in nature, enters the liver and spleen meridians, clears away heat and detoxicates, resolves phlegm and disperses stagnation, and is used for carbuncle, carbuncle, scrofula, phlegm nucleus, and lumps; Rhizoma Ligustici is bitter in nature, cold in taste, enters the spleen, stomach, liver, and gallbladder meridians, promotes blood circulation and removes blood stasis, promotes menstruation and relieves pain, eliminates accumulation, stops bleeding and reduces swelling, and is used for blood stasis, amenorrhea, lumps, bruises, bleeding from wounds, carbuncle, etc. The ministerial medicines are Radix Astragali and Radix Codonopsis. Astragalus, sweet in taste, slightly warm in nature, enters the spleen and lung meridians, strengthens the spleen and replenishes the middle, raises yang and lifts the sunken, benefits the defense and consolidates the exterior, and is mainly used to treat qi deficiency and fatigue, qi and blood deficiency, and middle qi sinking; Codonopsis, sweet in taste, neutral in nature, enters the spleen and lung meridians, strengthens the spleen and benefits the lungs, nourishes blood and produces body fluid, and is used for spleen and lung qi deficiency, lack of appetite and fatigue, cough and asthma, qi and blood deficiency, palpitations and shortness of breath; the adjuvant is honeysuckle. Honeysuckle, sweet in taste, cold in nature, enters the lung, heart, and stomach meridians, clears away heat and detoxifies, reduces inflammation and reduces swelling, and is used for carbuncle, furuncle, heat toxicity and bloody diarrhea, etc. The assistant drug is lily. Lily is sweet in taste and cold in nature. It enters the heart and lung meridians, nourishes yin and moistens the lungs, clears the heart and calms the mind. It is used for restlessness, palpitations, insomnia, dreaminess, mental confusion, etc. "Ben Cao": "Lily is sweet and mild in taste. It can treat abdominal distension caused by evil spirits, heart pain, promote bowel movements, tonify the middle and replenish qi. It grows in rivers and valleys." These medicines are used together to achieve the effect of detoxifying, dispersing nodules, replenishing qi and removing blood stasis.

[0031] The principle of Chinese medicine is based on the syndrome differentiation and treatment of traditional Chinese medicine. Through comprehensive research such as early theoretical excavation, case review, clinical epidemiological investigation and expert discussion, the inventor concluded that the occurrence and development of IHF is closely related to the "Qi deficiency, blood stasis and toxicity" in traditional Chinese medicine. Under the guidance of traditional Chinese medicine theory, the corresponding Chinese medicine combination is selected for symptomatic treatment to prevent and treat IHF Qi deficiency, blood stasis and toxicity syndrome safely and effectively. Based on this, the inventor has developed a Chinese medicine composition that can effectively treat IHF - the Yiqi Zhuyu Huadu formula.

[0032] At the same time, from the perspective of chemical analysis, a total of 45 compounds were identified in cultivated Trillium. Research shows that 23 compounds can interact with core targets such as TNF and exert anti-inflammatory effects through the PI3K / Akt and NF-κB signaling pathways.

[0033] It can be seen that the Chinese medicine composition for treating heart failure of the present invention conforms to the "monarch, minister, assistant and envoy" principle of traditional Chinese medicine, has fewer medicinal ingredients, has the advantages of high efficacy and no toxic side effects, and with the artificial production of Trillium, this Chinese medicine composition also has the advantage of low price and can be accepted by most patients, thereby further improving the promotion rate and having a good clinical application prospect.

[0034] Specifically, the Chinese medicine composition is prepared from the following raw materials in parts by weight: 3-30 parts of Trillium, 3-30 parts of Ligusticum wallichii, 10-40 parts of Astragalus, 10-30 parts of Codonopsis pilosula, 3-30 parts of Honeysuckle, and 3-20 parts of Lilium.

[0035] Exemplary, a traditional Chinese medicine composition for preventing and treating ischemic heart failure by inhibiting myocardial inflammatory response, wherein the raw materials of the traditional Chinese medicine composition include, by weight: 15 parts by weight of Trillium, 15 parts by weight of Ligusticum chuanxiong, 15 parts by weight of Astragalus, 10 parts by weight of Codonopsis pilosula, 15 parts by weight of Honeysuckle, and 10 parts by weight of Lilium.

[0036] Exemplary, a traditional Chinese medicine composition for preventing and treating ischemic heart failure by inhibiting myocardial inflammatory response, wherein the raw materials of the traditional Chinese medicine composition include, by weight: 10 parts by weight of Trillium gracile, 10 parts by weight of Rhizoma Cynomorii, 30 parts by weight of Radix Astragali, 15 parts by weight of Codonopsis pilosula, 15 parts by weight of Flos Lonicerae, and 10 parts by weight of Lilium.

[0037] Exemplary, a traditional Chinese medicine composition for preventing and treating ischemic heart failure by inhibiting myocardial inflammatory response, wherein the raw materials of the traditional Chinese medicine composition include, by weight: 20 parts by weight of Trillium, 20 parts by weight of Ligusticum chuanxiong, 10 parts by weight of Astragalus, 15 parts by weight of Codonopsis pilosula, 15 parts by weight of Honeysuckle, and 10 parts by weight of Lilium.

[0038] The basic formula composed of the raw materials of the above-mentioned traditional Chinese medicine composition of the present invention can be adjusted according to clinical symptoms on the basis of the above-mentioned traditional Chinese medicine composition during specific implementation, so as to achieve the purpose of improving the cardiac verification response of ischemic heart failure and improving certain special clinical symptoms, further enhance the adaptability of the composition of the present invention, and improve the therapeutic effect. These adjustments made based on the basic formula of the present invention are also within the protection scope of this application.

[0039] In the above-mentioned Chinese medicine combination, Trillium gracile, Rhizoma Coptidis, Radix Astragali, Codonopsis pilosula, Flos Lonicerae, and Lilium can be decocted in water and preferably crushed before decocting. However, it is not practical to crush the Chinese medicine in general use scenarios. Trillium gracile, as an anti-inflammatory agent, can be extracted with water and can also play a therapeutic role. However, due to its special properties, patients can swallow Trillium gracile directly, and the effect of direct swallowing is good with few side effects. Therefore, the above-mentioned Chinese medicine combination can be deeply processed to obtain a ready-to-eat Chinese medicine preparation for the prevention and treatment of ischemic heart failure.

[0040] The invention discloses a Chinese medicine preparation for preventing and treating ischemic heart failure, which contains solid particles of Tripterygium wilfordii and water extracts of Rhizoma Cynomorii, Radix Astragali, Radix Codonopsis pilosulae, Flos Lonicerae and Lilium. The Chinese medicine preparation can be eaten immediately.

[0041] In the Chinese medicine preparation of this embodiment, the radix trillium exists in the form of crushed particles, so that its efficacy can be maximized in the medicine, the anti-inflammatory effect is improved, and IHF is improved by inhibiting the inflammatory response. The water extracts of Rhizoma Cibotii, Radix Astragali, Radix Codonopsis, Flos Lonicerae, and Lilium assist the radix trillium in anti-inflammatory treatment, reduce the side effects of the radix trillium, replenish qi and strengthen the body of the patient, and improve the patient's survival status.

[0042] Instant Chinese medicine preparations can improve the portability of Chinese medicines, enable them to be widely promoted, and improve their application. According to actual needs, the instant Chinese medicine preparations for preventing and treating ischemic heart failure can also include pharmaceutically acceptable excipients and be made into various preparations according to conventional preparation processes, such as decoctions, granules, pills, capsules, tablets, powders, oral liquids and other dosage forms.

[0043] like Figure 1 As shown, the present invention provides a method for preparing a Chinese medicine preparation for preventing and treating ischemic heart failure, the preparation method comprising the following steps:

[0044] S100, weighing the Chinese medicine composition according to claim 1, freeze-drying the Psoralea corylifolia to obtain freeze-dried Psoralea corylifolia blocks, and cryogenically grinding the freeze-dried blocks to obtain Psoralea corylifolia fine powder;

[0045] S200, boiling a mixture of Rhizoma Cynanchum, Radix Astragali, Radix Codonopsis, Flos Lonicerae, and Bulbus Lilii with water for multiple times, combining the filtrates, and concentrating the filtrates under reduced pressure to obtain a medicinal solution;

[0046] S300, adding the Psoralea corylifolia powder into the medicinal solution to prepare the traditional Chinese medicine preparation.

[0047] The Chinese medicine preparation for preventing and treating ischemic heart failure can be prepared according to the following methods, such as method 1:

[0048] According to the formula, take Trillium gracile, Ligusticum wallichii, Astragalus membranaceus, Codonopsis pilosula, Honeysuckle and Lilium bulb, grind and mix them, add 6-10 times the weight of water, soak for 0.5-2 hours, then boil for 2-3 times, each time for 40-60 minutes, combine the decoctions, filter, and concentrate the filtrate under reduced pressure until each milliliter of liquid is equivalent to 0.2g-3g of the crude drug.

[0049] Method 2: Take Trillium gracile according to the formula and grind it into fine powder, combine it with the formula amount of Rhizoma Cynomorii, Radix Astragali, Codonopsis pilosula, Flos Lonicerae and Lilium, decoct it 2 to 3 times with 6 to 10 times the mass of water, each time for 40 to 60 minutes, combine the decoctions, filter, and concentrate the filtrate under reduced pressure until each milliliter of liquid is equivalent to 0.2 g to 3 g of the crude drug.

[0050] However, in the present invention, in order to improve the efficacy of the instant medicament, the medicament contains solid particles of Tripterygium wilfordii, and the specific method is as follows:

[0051] Take Trillium gracile according to the formula and grind it into fine powder for standby use; combine the formula amounts of Rhizoma Cibotii, Radix Astragali, Codonopsis pilosula, Flos Lonicerae and Lilium, and boil them 2 to 3 times with water of 6 to 10 times the weight of the total amount of the medicine, each time for 40 to 60 minutes, combine the decoctions, filter, and concentrate the filtrate under reduced pressure to the point where each milliliter of the medicine liquid is equivalent to 0.2 g to 3 g of the crude drug, and then add the Trillium gracile fine powder to the concentrated solution and mix well, so as to obtain a traditional Chinese medicine preparation for preventing and treating ischemic heart failure.

[0052] Freeze-dry the Trillium to obtain Trillium freeze-dried blocks, and crush the freeze-dried blocks to obtain Trillium fine powder.

[0053] The Chinese medicine preparation prepared by the present invention has the following effects:

[0054] (1) Improve the quality of life and TCM syndrome scores of patients with ischemic heart failure;

[0055] (2) Prevent and treat ischemic heart failure and improve cardiac function and hemodynamic indicators;

[0056] (3) reduce the level of NT-proBNP, a marker of heart failure;

[0057] (4) reduce myocardial infarction area;

[0058] (5) Inhibit cardiac inflammatory response after ischemic injury;

[0059] In addition, unless otherwise specified, the raw materials of the Chinese medicine composition of the present invention can be obtained commercially, and any range recorded in the present invention, including the end values ​​and any numerical value between the end values, and any sub-range of the Chinese medicine composition composed of the end values ​​or any numerical value between the end values, can achieve the purpose of inhibiting the cardiac inflammatory response after ischemic injury, thereby effectively preventing and treating ischemic heart failure.

[0060] In order to be able to be evenly mixed in the medicinal solution, the Trillium needs to be fully ground. Since Trillium is a block-like medicinal material, the finished medicinal material contains a small amount of water, which makes it difficult to grind and it is difficult to obtain a powdered medicinal material with sufficient particle size. Over-drying may cause the active ingredients in Trillium to flow out, greatly affecting its anti-inflammatory effect.

[0061] To this end, in order to obtain an instant medicine with uniform texture, the present invention first freeze-dries the Trillium gracile to remove moisture from the Trillium gracile and retain the active ingredients (alcohols, esters, etc.) therein, so that the texture of the Trillium gracile becomes brittle and easy to grind, thereby facilitating the acquisition of fine powder, improving the uniformity of the solid powder in subsequent medicines, and ensuring that the efficacy of each part of the medicine is consistent after packaging, thereby facilitating subsequent use and facilitating medical staff to predict the patient's state after using the medicine.

[0062] The following examples are provided to illustrate the efficacy of the medicament:

[0063] Embodiment 1:

[0064] Take Tripterygium wilfordii, Northern Liujinu, Astragalus, Codonopsis pilosula, Honeysuckle, Lily bulb, and water according to the formula;

[0065] Freeze-dry the Trillium gracile and bury it into powder for later use;

[0066] Combine the prescribed amount of northern liujinu, astragalus, codonopsis, honeysuckle and lily, decoct three times with water 6-10 times the weight of the total amount of the medicine, decoct for 40-60 minutes each time, combine the decoctions, filter, and concentrate the filtrate under reduced pressure until each milliliter of the medicine contains 1g of the crude drug (one tenth of the original filtrate), to obtain a concentrated solution;

[0067] Then add Trillium gracile powder into the concentrated solution and mix well to obtain the Chinese medicine preparation for preventing and treating ischemic heart failure.

[0068] Verification of the effect of Chinese medicine preparations in preventing and treating ischemic heart failure:

[0069] 1. The Yiqi Zhuyu Huadu formula can improve the quality of life of patients with ischemic heart failure

[0070] Before treatment, there was no statistical difference in the total score of Minnesota Heart Failure Life Quality Scale (MLHFQ) between the observation group and the control group (p>0.05), and the two groups were comparable. After treatment, the total score of MLHFQ in the observation group was lower than that in the control group, and the difference was statistically significant (p<0.05).

[0071] Table 1 Comparison of MLHFQ dimensions between the two groups before and after treatment

[0072]

[0073] Note: Compared with before treatment, * P<0.05; compared with the control group, # P<0.05.

[0074] 2. The Yiqi Zhuyu Huadu formula can improve the TCM syndrome score of patients with ischemic heart failure

[0075] Before treatment, there was no statistical difference in the TCM syndrome scores between the observation group and the control group, which was comparable (p>0.05). After treatment, the total effective rate of TCM syndrome in the observation group was higher than that in the control group, and the difference was statistically significant (p<0.05).

[0076] Table 2 The efficacy of TCM syndromes in the two groups before and after treatment

[0077]

[0078] Note: Compared with the control group, # P<0.05. Total effective rate = (markedly effective + effective) / total number of patients*100%.

[0079] 3. The Yiqi Zhuyu Huadu recipe can improve the cardiac function of mice with ischemic heart failure

[0080] In order to observe the pharmacological effect of Yiqi Zhuyu Huadu prescription on ischemic heart failure, an ischemic heart failure mouse model was established by ligation of the left anterior descending artery, and the mice with successful modeling were randomly divided into 3 groups, with 12 mice in each group. During the 2-week observation period, 3 mice (25%) died in the model group, 1 mouse (8%) treated with Yiqi Zhuyu Huadu prescription died, and 3 mice (25%) treated with the positive control drug captopril died. As shown in Table 3, compared with the sham operation group, the mice in the model group showed a significant decrease in left ventricular ejection fraction (EF), a decrease in short axis shortening fraction (FS), an increase in end-systolic ventricular internal diameter (LVIDs), an increase in end-diastolic ventricular internal diameter (LVIDd), an increase in end-systolic ventricular volume (LVVs), an increase in end-diastolic ventricular volume (LVVd), and an increase in ventricular mass (LVM) 2 weeks after modeling (P<0.01). Compared with the model group, the LVIDs, LVIDd, LVVs, LVVd, and LVM of mice treated with captopril were significantly reduced (P<0.01), but there was no statistical difference in EF and FS. Compared with the model group, the EF and FS of mice treated with Yiqi Zhuyu Huadu prescription were significantly increased (P<0.01), and LVIDs, LVIDd, LVVs, LVVd, and LVM were all reduced (P<0.05). The above results show that Yiqi Zhuyu Huadu prescription can effectively improve the cardiac function and ventricular remodeling of mice with ischemic heart failure and reduce the mortality rate after myocardial infarction.

[0081] Table 3 Effects of Yiqi Zhuyu Huadu prescription on cardiac function in mice with ischemic heart failure

[0082]

[0083]

[0084] Note: HR: heart rate; LVIDs, ventricular internal diameter at end-systole; LVIDd, ventricular internal diameter at end-diastole; LVVs, ventricular volume at end-systole; LVVd, ventricular volume at end-diastole; EF, ejection fraction; FS, fractional shortening; LVM, ventricular mass. △△ Compared with the sham operation group, P < 0.01; * Compared with the model group, P < 0.05; ** Compared with the model group, P<0.01.

[0085] 4. The prescription of replenishing qi, removing blood stasis and detoxifying can reduce the area of ​​myocardial infarction

[0086] Masson-stained heart sections were used to evaluate the effect of Yiqi Zhuyu Huadu prescription on myocardial infarction area in ischemic heart failure model mice. Figure 2 As shown, the myocardial infarction area of ​​mice treated with the Yiqi Zhuyu Huadu prescription was significantly reduced, and the therapeutic effect of the Yiqi Zhuyu Huadu prescription was better than that of the Yiqi Huoxue prescription.

[0087] 5. The Yiqi Zhuyu Huadu formula can inhibit the inflammatory response of the heart after ischemic injury

[0088] As shown in Table 4, the treatment of Yiqi Zhuyu Huadu prescription can significantly reduce the levels of serum inflammatory factors IL-6, IL-1β, and TNF-α in mice with ischemic heart failure (P<0.01). As shown in Table 5, the treatment of Yiqi Zhuyu Huadu prescription can significantly reduce the expression levels of cardiac inflammatory response-related genes TLR4, TRAF6, and NF-κB (P<0.05). The therapeutic effect of Yiqi Zhuyu Huadu prescription in inhibiting cardiac inflammation in ischemic heart failure is better than that of Yiqi Huoxue prescription.

[0089] Table 4 Effects of Yiqi Zhuyu Huadu prescription on serum inflammatory factors

[0090]

[0091] Note: IL-6: interleukin 6; IL-1β: interleukin 1β; TNF-α: tumor necrosis factor α. △△ Compared with the sham operation group, P < 0.01; * Compared with the model group, P < 0.05; ** Compared with the model group, P<0.01.

[0092] Table 5 Effects of Yiqi Zhuyu Huadu prescription on the expression of genes related to cardiac inflammatory response

[0093]

[0094]

[0095] Note: TLR4: Toll-like receptor 4; TRAF6: tumor necrosis factor receptor-associated factor 6; NF-κB: nuclear factor κB. △△ Compared with the sham operation group, P < 0.01; * Compared with the model group, P < 0.05; ** Compared with the model group, P<0.01.

[0096] 6. The Yiqi Zhuyu Huadu recipe can improve the hemodynamic parameters of zebrafish with heart failure

[0097] ISO was used to induce a zebrafish heart failure model. As shown in Table 6, after 4 hours of ISO stimulation, compared with the blank group zebrafish, the heart area, venous congestion area, blood flow rate and cardiac output of the zebrafish in the heart failure model group were increased (P<0.05).

[0098] Captopril treatment reduced the heart area, reduced the area of ​​venous congestion, increased blood flow velocity, and increased cardiac output in zebrafish (P<0.05), indicating that the heart failure model was successfully established.

[0099] Compared with the model group, the zebrafish treated with the Yiqi Zhuyu Huadu prescription had reduced heart area, reduced venous congestion area, increased blood flow velocity, and increased cardiac output (P<0.05).

[0100] The above results show that the Yiqi Zhuyu Huadu prescription can effectively improve the hemodynamic indicators of zebrafish heart failure model.

[0101] Table 6 Effects of Yiqi Zhuyu Huadu prescription on hemodynamics of zebrafish with heart failure

[0102]

[0103] Note: △ Compared with the sham operation group, P < 0.05; △△ Compared with the sham operation group, P < 0.01; * Compared with the model group, P < 0.05; ** Compared with the model group, P<0.01.

[0104] According to the examples, the Chinese medicine composition of the present invention has a rigorous formula, can inhibit excessive activation of cardiac inflammatory response in patients with ischemic heart failure, avoid further aggravation of myocardial damage, prevent and treat myocardial remodeling in ischemic heart failure by inhibiting cardiac inflammatory response, and improve patient prognosis. There is a clinical need to give full play to the characteristics and advantages of traditional Chinese medicine in the prevention and treatment of ischemic heart failure.

[0105] From the perspective of traditional Chinese medicine theory, "Qi deficiency, blood stasis and toxic accumulation" is one of the TCM syndrome characteristics of ischemic heart failure. The Chinese medicine composition of the present invention has the effects of detoxifying, dispersing stagnation, invigorating Qi and removing blood stasis, and has a good therapeutic effect on patients with Qi deficiency, blood stasis and toxic accumulation syndrome of ischemic heart failure.

[0106] Through the study of its pharmacological mechanism, this example found that it can improve the cardiac function of the ischemic heart failure mouse animal model and reduce the area of ​​myocardial infarction. Its mechanism may be to inhibit the excessive activation of cardiac inflammatory response and thus improve the myocardial remodeling of ischemic heart failure.

[0107] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present application.

Claims

1. A Chinese medicine combination for preventing and treating ischemic heart failure, characterized in that: The Chinese medicine composition is prepared from the following raw materials in parts by weight: 3-30 parts of Tripterygium wilfordii, 3-30 parts of Rhizoma Cynomorii, 10-40 parts of Radix Astragali, 10-30 parts of Codonopsis pilosula, 3-30 parts of Flos Lonicerae, and 3-20 parts of Bulbs of Lilium; The Chinese medicine composition is the raw material of a Chinese medicine preparation for immediate prevention and treatment of ischemic heart failure.

2. The Chinese medicine combination for preventing and treating ischemic heart failure according to claim 1, characterized in that: The traditional Chinese medicine preparation contains the water extract of Trillium gracile or solid particles of Trillium gracile, and the water extracts of Rhizoma Cynomorii, Radix Astragali, Radix Codonopsis pilosulae, Flos Lonicerae and Lilium gracile.

3. A method for preparing a Chinese medicine preparation for preventing and treating ischemic heart failure, characterized in that: The steps include: S100, weighing the Chinese medicine composition according to claim 1, and crushing the Psoralea corylifolia to obtain Psoralea corylifolia fine powder; S200, boiling a mixture of Rhizoma Cynanchum, Radix Astragali, Radix Codonopsis, Flos Lonicerae, and Bulbus Lilii with water for multiple times, combining the filtrates, and concentrating the filtrates under reduced pressure to obtain a medicinal solution; S300, adding the Psoralea corylifolia powder into the medicinal solution to prepare the traditional Chinese medicine preparation.

4. The method for preparing the Chinese medicine preparation for preventing and treating ischemic heart failure according to claim 3, characterized in that: In S200, the mass fraction of the water is 6-10 times that of the traditional Chinese medicine composition.

5. The method for preparing the Chinese medicine preparation for preventing and treating ischemic heart failure according to claim 3, characterized in that: In S200, the decoction is performed 2 to 3 times.

6. The method for preparing a Chinese medicine preparation for preventing and treating ischemic heart failure according to claim 3, characterized in that: In S200, each decoction takes 40 to 60 minutes.

7. The method for preparing a Chinese medicine preparation for preventing and treating ischemic heart failure according to claim 3, characterized in that: In S200, each milliliter of the drug solution contains 0.2 g to 3 g of the crude drug.

8. Use of the Chinese medicine combination as claimed in claim 1 in preventing and treating ischemic heart failure, characterized in that: The Chinese medicine combination can prevent and treat ischemic heart failure by inhibiting myocardial inflammatory response.

Images