Health-care feed additive for enhancing animal immunity and preparation method of health-care feed additive

Abstract

The invention provides a health-care feed additive for enhancing animal immunity and a preparation method thereof, and belongs to the field of feed additives. The preparation method comprises the following steps: preparing a composite enzymatic hydrolysate, preparing a composite extracting solution, preparing a pre-composite carrier, modifying the carrier, and mixing the preparation. The health-care feed additive capable of enhancing the animal immunity can effectively reduce the dosage of antibiotics in the animal feeding process, can improve the animal immunity through the synergistic and stable cooperation of various plant components and other raw materials, and can condition animal bodies in a health-care manner, and the utilization rate of the plant components is high; the stability, the anti-interference performance and the anti-oxidation performance of plant components in the feed additive are improved, and the storage resistance of the feed additive is improved.

Description

Technical Field

[0001] The invention relates to the field of feed additives, in particular to a health-care feed additive capable of enhancing animal immunity and a preparation method thereof. Background Art

[0002] In recent years, antibiotic feed additives have been widely used in the treatment and prevention of animal viral infections due to their advantages of rapid onset and good effects. However, the large-scale and long-term use of antibiotic feed additives not only harms the health of animals, but also the antibiotics will remain in the animals and eventually enter the human body through the food chain, posing a great threat to human health and posing huge risks to food safety and environmental safety.

[0003] Studies have shown that a large number of antibiotic ingredients are enriched in the human body through the food chain, which will lead to a decrease in the human immune function and increase the incidence of chronic diseases; in severe cases, it will also lead to immune diseases. At the same time, the large-scale and long-term use of antibiotic ingredients will lead to drug resistance in bacteria and the emergence of a large number of oxygen-resistant strains. In addition, while inhibiting pathogenic microorganisms, antibiotic feed additives will disrupt the pattern of mutual restraint between microbial populations or communities in animals, causing imbalance in the microecology of the flora, causing excessive reproduction of native bacteria or passing bacteria and location transfer, causing secondary infection or endogenous infection. In particular, the long-term and large-scale use of antibiotics will cause dysbiosis in the body. Harmful bacteria lurking in the animal's body take the opportunity to reproduce in large numbers, causing endogenous infection; although antibiotics will eliminate sensitive bacteria in the animal's body, they will cause a large number of vacancies on some microbial attachment points in the animal's body, providing an opportunity for external drug-resistant bacteria to take advantage of the situation.

[0004] In order to reduce the negative effects of antibiotics, starting from enhancing the animal's own immunity, adding a certain amount of non-antibiotic active ingredients to animal feed, reducing the occurrence of animal diseases and epidemic infectious diseases, reducing antibiotic residues, and avoiding the abuse of antibiotics are effective ways to improve the current breeding industry environment.

[0005] Studies have shown that plant extracts and probiotics, as the main ingredients of animal feed additives, can not only promote the healthy growth of animals, prevent infectious diseases, and improve animal immunity, but can also be used as therapeutic drug ingredients to solve problems such as bacterial infections and infectious disease outbreaks encountered during animal breeding. At the same time, this type of feed additive can also be used as an auxiliary agent for animal growth, regulate the animal's intestines, solve problems such as animal eating and food accumulation, and make the animal's hair smoother and brighter.

[0006] However, the pharmacological actions of different types of plant ingredients are not the same, and their coordination is unstable. In order to obtain better feed additive effects, it is difficult to screen and match the large number of existing plant ingredients. At the same time, it is difficult for multiple plant ingredients to coordinate with each other, and the utilization rate of the effective ingredients of existing plant ingredients is low, and they are not easily absorbed and converted. In order to ensure that the plant ingredients achieve their due effects, it is necessary to add a large number of plant ingredients (such as plant dry powder, plant extracts, etc.) to animal feed, which directly leads to a decrease in the palatability of animal feed and a decrease in the average daily feed intake of animals. This not only limits the role of plant ingredients and fails to effectively improve animal immunity, but may also stimulate the gastrointestinal tract of animals, causing vomiting and diarrhea in animals.

[0007] At the same time, existing plant ingredients (such as plant dry powder, plant extracts, etc.) have poor stability, anti-interference and antioxidant properties. They are not only easily ineffective during the processing and preparation of feed additives, or are affected by other ingredients in animal feed; they also lead to poor storage resistance of feed additives, and the immunity-enhancing and health-care effects on animals are reduced after long-term storage. Summary of the invention

[0008] In order to solve the technical problems existing in the prior art, the present invention provides a health-care feed additive for enhancing animal immunity and a preparation method thereof, which can effectively reduce the amount of antibiotics used in the animal breeding process, can coordinate and stably cooperate with a variety of plant components and other raw materials, and can improve the animal immunity while maintaining health and conditioning the animal body, and the utilization rate of the plant components is high; and the stability, anti-interference and antioxidant properties of the plant components in the feed additive are improved, and the storage resistance of the feed additive is improved.

[0009] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows: A method for preparing a health-care feed additive for enhancing animal immunity, comprising the following steps: preparing a composite enzymatic hydrolysate, preparing a composite extract, preparing a pre-composite carrier, modifying the carrier, and preparing a mixed preparation; The method for preparing the composite enzymatic hydrolysate comprises the following steps: respectively crushing dried Poria cocos and Sophora flavescens into Poria cocos powder and Sophora flavescens powder; extracting the Poria cocos powder and Sophora flavescens powder with a sodium hydroxide solution, precipitating, and separating to obtain a solid; enzymatically hydrolyzing the solid with papain and trypsin, and then inactivating the enzymes to obtain the composite enzymatic hydrolysate; The method for preparing the composite extract comprises: crushing dried inulin and licorice into inulin powder and licorice powder respectively; extracting the inulin powder and licorice powder with ethanol solution, and concentrating the extract to obtain the composite extract; The method for preparing the pre-composite carrier comprises the following steps: adding defatted rice bran and defatted bone meal into a pre-treatment liquid, heating the liquid to 40-45° C., keeping the temperature and stirring, separating and obtaining a solid, washing and drying the solid, and obtaining the pre-treatment carrier; then adding the pre-treatment carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, and 2,2'-bipyrimidine into a methanol solution, mixing the mixture evenly, adding potassium oxalate, keeping the temperature and stirring at 42-45° C., separating and obtaining a solid, washing and drying the solid, and obtaining the pre-composite carrier; The pretreatment liquid is an isopropanol aqueous solution in which erucamidopropyltrimethylammonium chloride is dispersed; The carrier modification method comprises: performing carrier modification treatment on the pre-composite carrier using N,N,N-trimethylglycine in a solvent environment to obtain a modified carrier; The mixed preparation method comprises the following steps: evenly mixing the composite enzymatic hydrolysate and the composite extract, adding the modified carrier, stirring at room temperature, separating the solid matter, and drying to obtain the composite plant component; evenly mixing the composite plant component, Bifidobacterium lactis powder, and Lactobacillus plantarum powder to obtain the health feed additive capable of enhancing animal immunity.

[0010] Preferably, in the preparation of the pre-composite carrier, the defatted rice bran and defatted bone meal are kept in the pre-treatment liquid for stirring for 17-19 hours; The weight ratio of defatted rice bran, defatted bone meal and pretreatment liquid is 5-6:1.3-1.5:80-85; In the pretreatment liquid, the weight percentage content of erucamide propyltrimethylammonium chloride is 1.6-1.8wt%, and the volume concentration of the isopropanol aqueous solution is 32-34%.

[0011] Preferably, in the preparation of the pre-composite carrier, the stirring time at 42-45°C is 10-12h; The weight ratio of the pretreated carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, 2 2'-bipyrimidine, methanol solution, and potassium oxalate is 16-16.5:2-2.2:0.7-0.8:3.6-4.1:95-100:3.2-3.3; The volume concentration of the methanol solution is 50-55%.

[0012] Furthermore, the method for modifying the carrier is to add N,N,N-trimethylglycine to an ethanol solution, mix well, add a pre-composite carrier, heat to 35-40°C, keep warm and stir for 5-6 hours, separate and obtain a solid, wash and dry the solid to obtain a modified carrier.

[0013] Preferably, in the carrier modification, the solvent is an ethanol solution with a volume concentration of 30-35%; The weight ratio of N,N,N-trimethylglycine, solvent and pre-composite carrier is 2-2.2:100:10-11.

[0014] Preferably, in the mixed preparation, the stirring time at room temperature after adding the modified carrier is 8-10 hours; The weight ratio of the composite enzymatic hydrolysate, the composite extract and the modified carrier is 30-35:65-70:18-20; The weight ratio of the composite plant component, the lactobacillus powder and the plant lactobacillus powder is 100:3.1-3.2:1.9-2.

[0015] Preferably, in the preparation of the composite enzymatic hydrolysate, the mass concentration of the sodium hydroxide solution is 0.04-0.05wt%; The temperature of extracting Poria cocos powder and Sophora flavescens powder with sodium hydroxide solution is 35-40℃, and the extraction time is 1.5-2.5h; The weight ratio of Poria cocos powder, Sophora flavescens powder and sodium hydroxide solution is 0.7-0.8:1.5-1.6:18-20.

[0016] Preferably, in the preparation of the composite enzymatic hydrolysis solution, the enzymatic hydrolysis temperature is 40-45°C and the enzymatic hydrolysis time is 8-10h; The weight ratio of solid matter, papain and trypsin is 100-105:3-4:1.2-1.6.

[0017] Preferably, in the preparation of the composite extract, the extraction temperature is 80-85°C and the extraction time is 2-2.5h; The weight ratio of the micropowder of Inula officinalis, the micropowder of Licorice and the ethanol solution is 2-2.2:4.5-4.6:115-120; The volume concentration of the ethanol solution is 65-70%.

[0018] A health-care feed additive for enhancing animal immunity is prepared by the above-mentioned preparation method.

[0019] Compared with the prior art, the present invention has the following beneficial effects: (1) The preparation method of the health-care feed additive for enhancing animal immunity of the present invention comprises the following steps: extracting and precipitating Poria cocos and Sophora flavescens with sodium hydroxide solution, and then enzymolyzing the precipitate with papain and trypsin to obtain a composite enzymolysis solution; extracting and concentrating Inula officinalis and Licorice with alcohol and water to obtain a composite extract solution; and then, in the step of preparing a pre-composite carrier, selecting defatted rice bran and defatted bone meal as carrier matrix materials, and treating them with a pre-treatment solution containing erucamidopropyl trimethyl ammonium chloride, and adjusting the defatted rice bran and defatted bone meal with erucamidopropyl trimethyl ammonium chloride. The surface charge distribution of the powder and the modification effect on its surface can improve the binding stability of the pre-treated carrier and the iron / zinc-based composite organic metal framework material, improve the storage resistance of the feed additive, and enable the feed additive to maintain its original performance after long-term storage; the pre-treated carrier is combined with the iron / zinc-based composite organic metal framework material in an in-situ composite manner to obtain a pre-composite carrier; the large specific surface and multi-porous characteristics of the composite carrier can effectively and stably combine with the plant active ingredients, thereby achieving effective protection of the plant active ingredients, improving the anti-interference and antioxidant capabilities of the plant active ingredients, and avoiding the active ingredients in the feed. The pre-composite carrier can prevent the ineffectiveness of the feed additive during processing, preparation and storage, avoid the ineffectiveness of other ingredients in animal feed, supplement the nutritional ingredients of the feed additive, and improve the utilization rate of the effective ingredients in the feed additive; at the same time, the pre-composite carrier can also improve the absorption of the effective ingredients in the animal's stomach and intestines; then in the carrier modification step, N,N,N-trimethylglycine is used to modify the pre-composite carrier to further improve the binding performance of the modified carrier with the effective ingredients in the composite enzymatic hydrolysate and composite extract, and realize the effective coordination of various plant components, and improve gastrointestinal digestion and absorption, and further improve the immunity of animals; Finally, in the mixed preparation step, a composite plant component containing effective ingredients of Poria cocos, Sophora flavescens, Inula japonica and Licorice is combined with Bifidobacterium lactis powder and Lactobacillus plantarum powder to prepare a health-care feed additive for enhancing animal immunity. Various technical means cooperate with each other and work synergistically to effectively reduce the amount of antibiotics used in the animal breeding process. A variety of plant components can be stably coordinated with other raw materials to improve animal immunity while maintaining health and conditioning the animal body, with a high utilization rate of plant components. The stability, anti-interference and antioxidant properties of the plant components in the feed additive are improved, and the storage resistance of the feed additive is improved.

[0020] (2) The health-care feed additive for enhancing animal immunity of the present invention is used in feeding white-feathered broiler chickens. When the amount of the feed additive added is 2% of the weight of conventional feed, the initial average body weight of the broiler chickens (day 0) is 204.9 g, which can increase to 532.3 g after 7 days of feeding, 810.6 g after 14 days of feeding, and 13115.8 g after 21 days of feeding. At the same time, it can effectively improve the Newcastle disease antibody level of the broiler chickens and improve the immunity of the broiler chickens. It can also effectively improve the brightness of the feathers of the white-feathered broiler chickens, and will not affect the normal eating of the broiler chickens during the feeding process, and can effectively prevent diarrhea in the broiler chickens.

[0021] (3) The health-care feed additive for enhancing animal immunity of the present invention is used in feeding pet dogs. When the amount of the feed additive added is 3% of the weight of conventional feed, after feeding for 30 days, the immune protein IgA content in the pet dog can reach 1.67 g / L, the IgG content can reach 6.75 g / L, and the IgM content can reach 2.19 g / L, which can effectively improve the immunity of the pet dog. At the same time, it can also improve the intestinal environment of the pet dog, increase the content of beneficial bacteria (Lactobacillus, Bifidobacterium) in its intestine, reduce the content of harmful bacteria (Escherichia coli), and improve the coat quality of the pet dog.

[0022] (4) After testing, the health-care feed additive for enhancing animal immunity of the present invention was placed in an environment with a temperature of 40°C and a relative humidity of 80%, and was used for feeding pet dogs after being left to stand for 120 days. After 30 days of feeding, the content of immune proteins IgA, IgG, and IgM in the pet dogs was not significantly different from that of the feed additive that had not been stored. The feed additive has good storage resistance and can maintain its performance for a long time. DETAILED DESCRIPTION

[0023] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation methods of the present invention are now described.

[0024] The invention provides a method for preparing a health-care feed additive for enhancing animal immunity, which comprises the following steps: preparing a composite enzymatic hydrolysate, preparing a composite extract, preparing a pre-composite carrier, modifying the carrier, and preparing a mixed preparation.

[0025] The method for preparing the composite enzymatic hydrolysate comprises placing Poria cocos and Sophora flavescens in a constant temperature drying oven, drying at a constant temperature of 60-70°C until the moisture content is less than 5wt%, and crushing them to 50-60 meshes respectively to obtain Poria cocos powder and Sophora flavescens powder; then putting the Poria cocos powder and Sophora flavescens powder into a sodium hydroxide solution with a mass concentration of 0.04-0.05wt%, stirring and heating to 35-40°C, extracting with stirring and keeping warm for 1.5-2.5h, and centrifuging at 5000-6000rpm to obtain a centrifugal supernatant; adjusting the pH of the centrifugal supernatant to 4.5-5.0 with hydrochloric acid, letting it stand for precipitation for 10-12h, and centrifuging at 7000-8000rpm to obtain a solid, washing the solid with water until it is neutral, putting the solid into 8-9 times the weight of deionized water, and then adding papain and trypsin, keeping warm at 40-45°C for enzymatic hydrolysis for 8-10h, heating to inactivate the enzymes, and cooling to obtain the composite enzymatic hydrolysate.

[0026] In the preparation of the composite enzymatic hydrolysate, the weight ratio of Poria cocos powder, Sophora flavescens powder and sodium hydroxide solution is 0.7-0.8:1.5-1.6:18-20; The weight ratio of solid matter, papain, and trypsin is 100-105:3-4:1.2-1.6; The enzymatic activity of papain is 100,000 U / g; the enzymatic activity of trypsin is 4000 U / g.

[0027] The method for preparing the composite extract comprises placing inulin and licorice in a constant temperature drying oven, drying at a constant temperature of 60-70°C until the moisture content is less than 5wt%, and crushing them to 50-60 meshes respectively to obtain inulin powder and licorice powder; then adding the inulin powder and licorice powder into an ethanol solution with a volume concentration of 65-70%, stirring and heating to 80-85°C, keeping the temperature under reflux and stirring for 2-2.5h, filtering to remove solids, transferring the filtrate into a vacuum concentrator, and concentrating the filtrate at 60-65°C to 45-50% of the original volume of the filtrate under a vacuum degree of 0.08-0.09MPa to obtain the composite extract.

[0028] In the preparation of the composite extract, the weight ratio of the inula powder, the liquorice powder and the ethanol solution is 2-2.2:4.5-4.6:115-120.

[0029] The method for preparing the pre-composite carrier comprises the following steps: adding defatted rice bran and defatted bone meal into a pre-treatment liquid, stirring and heating to 40-45° C., keeping the temperature and stirring for 17-19 hours, naturally cooling to room temperature, centrifuging to obtain solid matter, washing the solid matter 2-3 times with deionized water of 6-7 times the weight, and drying to obtain the pre-treatment carrier; then adding the pre-treatment carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, and 2,2'-bipyrimidine into a methanol solution with a volume concentration of 50-55%, stirring for 15-30 minutes, and then adding potassium oxalate, keeping the temperature and stirring at 42-45° C. for 10-12 hours, and centrifuging to obtain solid matter, washing the solid matter with anhydrous methanol and deionized water in turn, and placing the solid matter in a vacuum drying oven, and drying the solid matter at 60-65° C. to a constant weight under a vacuum degree of 0.08-0.09 MPa to obtain the pre-composite carrier.

[0030] In the preparation of the pre-composite carrier, the weight ratio of defatted rice bran, defatted bone meal and pre-treatment liquid is 5-6:1.3-1.5:80-85; The pretreatment liquid is an isopropanol aqueous solution in which erucamidopropyl trimethylammonium chloride is dispersed; in the pretreatment liquid, the weight percentage content of erucamidopropyl trimethylammonium chloride is 1.6-1.8wt%, and the volume concentration of the isopropanol aqueous solution is 32-34%.

[0031] In the preparation of the pre-composite carrier, the weight ratio of the pre-treated carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, 2 2'-bipyrimidine, methanol solution and potassium oxalate is 16-16.5:2-2.2:0.7-0.8:3.6-4.1:95-100:3.2-3.3.

[0032] The carrier modification method comprises the following steps: adding N,N,N-trimethylglycine to an ethanol solution with a volume concentration of 30-35%, stirring for 10-20 minutes, then adding a pre-composite carrier, stirring and heating to 35-40° C., keeping the temperature and stirring for 5-6 hours, and then centrifuging to obtain a solid, washing the solid with 4-5 times the weight of deionized water, placing the solid in a vacuum drying oven, and drying at 60-65° C. to a constant weight under a vacuum degree of 0.08-0.09 MPa to obtain a modified carrier.

[0033] In the carrier modification, the weight ratio of N,N,N-trimethylglycine, ethanol solution and pre-composite carrier is 2-2.2:100:10-11.

[0034] The mixed preparation method comprises the following steps: evenly mixing the composite enzymatic hydrolysate and the composite extract, adding the modified carrier, stirring at room temperature for 8-10 hours, centrifuging to obtain solid matter, placing the solid matter in a vacuum drying oven, drying to constant weight at a vacuum degree of 0.08-0.09 MPa and 60-65° C. to obtain the composite plant component; and evenly mixing the composite plant component, Bifidobacterium lactis powder and Lactobacillus plantarum powder to obtain the health feed additive capable of enhancing animal immunity.

[0035] In the mixed preparation, the weight ratio of the composite enzymatic hydrolysate, the composite extract, and the modified carrier is 30-35:65-70:18-20; The weight ratio of the composite plant component, the Bifidobacterium lactis powder, and the Lactobacillus plantarum powder is 100:3.1-3.2:1.9-2; The effective bacterial concentration of Bifidobacterium lactis powder is ≥10 billion cfu / g; the effective bacterial concentration of Lactobacillus plantarum powder is ≥10 billion cfu / g.

[0036] The present invention also provides a health-care feed additive for enhancing animal immunity prepared by the above method.

[0037] Example 1 This embodiment provides a method for preparing a health-care feed additive for enhancing animal immunity, which comprises the following steps: 1. Preparation of compound enzymatic solution Poria cocos and Sophora flavescens were placed in a constant temperature drying oven, dried at 60°C until the moisture content was less than 5wt%, and crushed to 50 meshes respectively to obtain Poria cocos powder and Sophora flavescens powder; then the Poria cocos powder and Sophora flavescens powder were put into a sodium hydroxide solution with a mass concentration of 0.04wt%, stirred and heated to 35°C, extracted with insulation and stirring for 1.5h, and centrifuged at 5000rpm to obtain a centrifugal supernatant; after adjusting the pH of the centrifugal supernatant to 4.5 with hydrochloric acid, the solution was allowed to stand for 10h, and centrifuged at 7000rpm to obtain a solid, washed the solid with water to neutrality, put the solid into 8 times the weight of deionized water, and then added papain and trypsin, and after enzymatic hydrolysis at 40°C for 8h, the solution was heated to inactivate the enzyme and then cooled to obtain a composite enzymatic hydrolyzate.

[0038] Among them, the weight ratio of Poria cocos powder, Sophora flavescens powder and sodium hydroxide solution is 0.7:1.5:18.

[0039] The weight ratio of solid matter, papain and trypsin is 100:3:1.2.

[0040] The enzymatic activity of papain is 100,000 U / g; the enzymatic activity of trypsin is 4000 U / g.

[0041] 2. Preparation of composite extract The Inula and Licorice were placed in a constant temperature drying oven, dried at 60°C until the moisture content was less than 5wt%, and then crushed to 50 mesh, respectively, to obtain Inula powder and Licorice powder; then the Inula powder and Licorice powder were added to an ethanol solution with a volume concentration of 65%, stirred and heated to 80°C, kept refluxed and stirred for 2 hours, filtered to remove solids, and the filtrate was transferred to a vacuum concentrator, and concentrated at 60°C to 45% of the original volume of the filtrate under a vacuum degree of 0.08MPa to obtain a composite extract.

[0042] Among them, the weight ratio of inula odorata powder, licorice powder and ethanol solution is 2:4.5:115.

[0043] 3. Preparation of pre-composite carrier Defatted rice bran and defatted bone meal were put into the pretreatment liquid, stirred and heated to 40°C, kept warm and stirred for 17 hours, then naturally cooled to room temperature, centrifuged to obtain solids, washed the solids twice with 6 times the weight of deionized water, and dried to obtain a pretreatment carrier; then the pretreatment carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, and 2,2'-bipyrimidine were put into a methanol solution with a volume concentration of 50%, stirred for 15 minutes, and potassium oxalate was continued to be added. After keeping warm and stirring at 42°C for 10 hours, solids were obtained by centrifugation. The solids were washed with anhydrous methanol and deionized water in turn, placed in a vacuum drying oven, and dried to constant weight at 60°C under a vacuum degree of 0.08MPa to obtain a pre-composite carrier.

[0044] Among them, the weight ratio of defatted rice bran, defatted bone meal and pretreatment liquid is 5:1.3:80.

[0045] The pretreatment liquid is an isopropanol aqueous solution in which erucamidopropyl trimethylammonium chloride is dispersed; in the pretreatment liquid, the weight percentage content of erucamidopropyl trimethylammonium chloride is 1.6wt%, and the volume concentration of the isopropanol aqueous solution is 32%.

[0046] The weight ratio of the pretreated carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, 2 2'-bipyrimidine, methanol solution and potassium oxalate is 16:2:0.7:3.6:95:3.2.

[0047] 4. Carrier modification N,N,N-trimethylglycine was added to an ethanol solution with a volume concentration of 30%, and after stirring for 10 minutes, the pre-composite carrier was continued to be added, and the temperature was raised to 35°C with stirring. After keeping warm and stirring for 5 hours, the solid was obtained by centrifugation. The solid was washed with 4 times the weight of deionized water, placed in a vacuum drying oven, and dried at 60°C to constant weight under a vacuum degree of 0.08 MPa to obtain a modified carrier.

[0048] Wherein, the weight ratio of N,N,N-trimethylglycine, ethanol solution and pre-composite carrier is 2:100:10.

[0049] 5. Mixed preparations The composite enzymatic hydrolysate and the composite extract are mixed evenly, and then added into the modified carrier. After stirring at room temperature for 8 hours, the solid matter is separated by centrifugation, and the solid matter is placed in a vacuum drying oven, and dried to constant weight at a vacuum degree of 0.08 MPa and 60° C. to obtain a composite plant component; then the composite plant component, Bifidobacterium lactis powder, and Lactobacillus plantarum powder are mixed evenly to obtain a health feed additive for enhancing animal immunity.

[0050] Among them, the weight ratio of the composite enzymatic hydrolysate, the composite extract and the modified carrier is 30:70:18.

[0051] The weight ratio of the composite plant component, the Bifidobacterium lactis powder and the Lactobacillus plantarum powder is 100:3.1:1.9.

[0052] The Bifidobacterium lactis powder used in this embodiment was purchased from Zhongke Jiayi Nutritional Medicine (Shandong) Microecology Research Institute Co., Ltd., with an effective bacterial concentration of 10 billion cfu / g; the Lactobacillus plantarum powder used was purchased from Zhongke Jiayi Nutritional Medicine (Shandong) Microecology Research Institute Co., Ltd., with an effective bacterial concentration of 10 billion cfu / g.

[0053] This embodiment also provides a health-care feed additive for enhancing animal immunity prepared by the aforementioned method.

[0054] Example 2 This embodiment provides a method for preparing a health-care feed additive for enhancing animal immunity, which comprises the following steps: 1. Preparation of compound enzymatic solution Poria cocos and Sophora flavescens were placed in a constant temperature drying oven, dried at 65°C until the moisture content was less than 5wt%, and crushed to 55 meshes respectively to obtain Poria cocos powder and Sophora flavescens powder; then the Poria cocos powder and Sophora flavescens powder were put into a sodium hydroxide solution with a mass concentration of 0.042wt%, stirred and heated to 37°C, extracted with insulation and stirring for 2h, and centrifuged at 5500rpm to obtain a centrifugal supernatant; after adjusting the pH of the centrifugal supernatant to 4.6 with hydrochloric acid, the solution was allowed to stand for 11h, and centrifuged at 7500rpm to obtain a solid, which was washed with water to neutrality, and then put into 8.5 times the weight of deionized water, and then papain and trypsin were added, and after enzymatic hydrolysis at 42°C for 9h, the solution was heated to inactivate the enzyme and then cooled to obtain a composite enzymatic hydrolyzate.

[0055] Among them, the weight ratio of Poria cocos powder, Sophora flavescens powder and sodium hydroxide solution is 0.75:1.58:19.

[0056] The weight ratio of solid matter, papain and trypsin is 102:3.5:1.3.

[0057] The enzymatic activity of papain is 100,000 U / g; the enzymatic activity of trypsin is 4000 U / g.

[0058] 2. Preparation of composite extract The Inula and Licorice were placed in a constant temperature drying oven, dried at 65°C until the moisture content was less than 5wt%, and then crushed to 55 mesh, respectively, to obtain Inula powder and Licorice powder; then the Inula powder and Licorice powder were added to an ethanol solution with a volume concentration of 68%, stirred and heated to 83°C, kept refluxed and stirred for 2.2 hours, filtered to remove solids, and the filtrate was transferred to a vacuum concentrator, and concentrated at 62°C to 50% of the original volume of the filtrate under a vacuum degree of 0.085MPa to obtain a composite extract.

[0059] Among them, the weight ratio of inula odorata powder, licorice powder and ethanol solution is 2.1:4.6:117.

[0060] 3. Preparation of pre-composite carrier Defatted rice bran and defatted bone meal were added to the pretreatment liquid, stirred and heated to 42°C, kept warm and stirred for 18 hours, then naturally cooled to room temperature, centrifuged to obtain solids, washed the solids three times with 6.5 times the weight of deionized water, and dried to obtain a pretreatment carrier; then the pretreatment carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, and 2,2'-bipyrimidine were added to a methanol solution with a volume concentration of 53%, stirred for 20 minutes, and potassium oxalate was added. After keeping warm and stirring at 44°C for 11 hours, solids were obtained by centrifugation. The solids were washed with anhydrous methanol and deionized water in turn, placed in a vacuum drying oven, and dried to constant weight at 62°C under a vacuum degree of 0.085MPa to obtain a pre-composite carrier.

[0061] Among them, the weight ratio of defatted rice bran, defatted bone meal and pretreatment liquid is 5.5:1.4:83.

[0062] The pretreatment liquid is an isopropanol aqueous solution in which erucamidopropyl trimethylammonium chloride is dispersed; in the pretreatment liquid, the weight percentage content of erucamidopropyl trimethylammonium chloride is 1.7wt%, and the volume concentration of the isopropanol aqueous solution is 33%.

[0063] The weight ratio of the pretreated carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, 2 2'-bipyrimidine, methanol solution and potassium oxalate is 16.3:2.1:0.75:3.9:96:3.25.

[0064] 4. Carrier modification N,N,N-trimethylglycine was added to an ethanol solution with a volume concentration of 32%, and after stirring for 15 minutes, the pre-composite carrier was continued to be added, and the temperature was raised to 38°C with stirring. After keeping warm and stirring for 5.5 hours, the solid was obtained by centrifugation. The solid was washed with 5 times the weight of deionized water, placed in a vacuum drying oven, and dried at 62°C to constant weight under a vacuum degree of 0.085 MPa to obtain a modified carrier.

[0065] Wherein, the weight ratio of N,N,N-trimethylglycine, ethanol solution and pre-composite carrier is 2.1:100:10.6.

[0066] 5. Mixed preparations The composite enzymatic hydrolysate and the composite extract are mixed evenly, and then added into the modified carrier. After stirring at room temperature for 9 hours, the solid matter is separated by centrifugation, and the solid matter is placed in a vacuum drying oven, and dried to constant weight at a vacuum degree of 0.085 MPa and 62° C. to obtain a composite plant component; then the composite plant component, Bifidobacterium lactis powder, and Lactobacillus plantarum powder are mixed evenly to obtain a health-care feed additive for enhancing animal immunity.

[0067] Among them, the weight ratio of the composite enzymatic hydrolysate, the composite extract and the modified carrier is 33:67:19.

[0068] The weight ratio of the composite plant component, the Bifidobacterium lactis powder and the Lactobacillus plantarum powder is 100:3.15:1.96.

[0069] The Bifidobacterium lactis powder used in this embodiment was purchased from Zhongke Jiayi Nutritional Medicine (Shandong) Microecology Research Institute Co., Ltd., with an effective bacterial concentration of 10 billion cfu / g; the Lactobacillus plantarum powder used was purchased from Zhongke Jiayi Nutritional Medicine (Shandong) Microecology Research Institute Co., Ltd., with an effective bacterial concentration of 10 billion cfu / g.

[0070] This embodiment also provides a health-care feed additive for enhancing animal immunity prepared by the aforementioned method.

[0071] Example 3 This embodiment provides a method for preparing a health-care feed additive for enhancing animal immunity, which comprises the following steps: 1. Preparation of compound enzymatic solution Poria cocos and Sophora flavescens were placed in a constant temperature drying oven, dried at 70°C until the moisture content was less than 5wt%, and crushed to 60 meshes respectively to obtain Poria cocos powder and Sophora flavescens powder; then the Poria cocos powder and Sophora flavescens powder were put into a sodium hydroxide solution with a mass concentration of 0.05wt%, stirred and heated to 40°C, extracted with insulation and stirring for 2.5 hours, and centrifuged at 6000rpm to obtain a centrifugal supernatant; after adjusting the pH of the centrifugal supernatant to 5.0 with hydrochloric acid, the solution was allowed to stand for 12 hours, and centrifuged at 8000rpm to obtain a solid, washed the solid with water to neutrality, put the solid into 9 times the weight of deionized water, and then added papain and trypsin, and after enzymatic hydrolysis at 45°C for 10 hours, the solution was heated to inactivate the enzyme, and then cooled to obtain a composite enzymatic hydrolyzate.

[0072] Among them, the weight ratio of Poria cocos powder, Sophora flavescens powder and sodium hydroxide solution is 0.8:1.6:20.

[0073] The weight ratio of solid matter, papain and trypsin is 105:4:1.6.

[0074] The enzymatic activity of papain is 100,000 U / g; the enzymatic activity of trypsin is 4000 U / g.

[0075] 2. Preparation of composite extract The Inula and Licorice were placed in a constant temperature drying oven, dried at 70°C until the moisture content was less than 5wt%, and then crushed to 60 mesh to obtain Inula powder and Licorice powder respectively; then the Inula powder and Licorice powder were added to an ethanol solution with a volume concentration of 70%, stirred and heated to 85°C, kept refluxed and stirred for 2.5 hours, filtered to remove solids, and the filtrate was transferred to a vacuum concentrator, concentrated at 65°C to 50% of the original volume of the filtrate under a vacuum degree of 0.09MPa to obtain a composite extract.

[0076] Among them, the weight ratio of inula odorata powder, licorice powder and ethanol solution is 2.2:4.6:120.

[0077] 3. Preparation of pre-composite carrier Defatted rice bran and defatted bone meal were put into the pretreatment liquid, stirred and heated to 45°C, kept warm and stirred for 19 hours, then naturally cooled to room temperature, centrifuged to obtain solids, washed the solids three times with 7 times the weight of deionized water, and dried to obtain a pretreatment carrier; then the pretreatment carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, and 2,2'-bipyrimidine were put into a methanol solution with a volume concentration of 55%, stirred for 30 minutes, and potassium oxalate was added. After keeping warm and stirring at 45°C for 12 hours, solids were obtained by centrifugation. The solids were washed with anhydrous methanol and deionized water in turn, placed in a vacuum drying oven, and dried to constant weight at 65°C under a vacuum degree of 0.09 MPa to obtain a pre-composite carrier.

[0078] Among them, the weight ratio of defatted rice bran, defatted bone meal and pretreatment liquid is 6:1.5:85.

[0079] The pretreatment liquid is an isopropanol aqueous solution in which erucamide propyl trimethyl ammonium chloride is dispersed; in the pretreatment liquid, the weight percentage content of erucamide propyl trimethyl ammonium chloride is 1.8wt%, and the volume concentration of the isopropanol aqueous solution is 34%.

[0080] The weight ratio of the pretreated carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, 2 2'-bipyrimidine, methanol solution and potassium oxalate is 16.5:2.2:0.8:4.1:100:3.3.

[0081] 4. Carrier modification N,N,N-trimethylglycine was added to an ethanol solution with a volume concentration of 35%, and after stirring for 20 minutes, the pre-composite carrier was continued to be added, and the temperature was raised to 40°C with stirring. After keeping warm and stirring for 6 hours, the solid was obtained by centrifugation. The solid was washed with 5 times the weight of deionized water, placed in a vacuum drying oven, and dried at 65°C to constant weight under a vacuum degree of 0.09 MPa to obtain a modified carrier.

[0082] Wherein, the weight ratio of N,N,N-trimethylglycine, ethanol solution and pre-composite carrier is 2.2:100:11.

[0083] 5. Mixed preparations The composite enzymatic hydrolysate and the composite extract are mixed evenly, and then added into the modified carrier. After stirring at room temperature for 10 hours, the solid matter is separated by centrifugation, and the solid matter is placed in a vacuum drying oven, and dried to constant weight at a vacuum degree of 0.09 MPa and 65° C. to obtain a composite plant component; then the composite plant component, Bifidobacterium lactis powder, and Lactobacillus plantarum powder are mixed evenly to obtain a health feed additive for enhancing animal immunity.

[0084] Among them, the weight ratio of the composite enzymatic hydrolysate, the composite extract and the modified carrier is 35:65:20.

[0085] The weight ratio of the composite plant component, the Bifidobacterium lactis powder and the Lactobacillus plantarum powder is 100:3.2:2.

[0086] The Bifidobacterium lactis powder used in this embodiment was purchased from Zhongke Jiayi Nutritional Medicine (Shandong) Microecology Research Institute Co., Ltd., with an effective bacterial concentration of 10 billion cfu / g; the Lactobacillus plantarum powder used was purchased from Zhongke Jiayi Nutritional Medicine (Shandong) Microecology Research Institute Co., Ltd., with an effective bacterial concentration of 10 billion cfu / g.

[0087] This embodiment also provides a health-care feed additive for enhancing animal immunity prepared by the aforementioned method.

[0088] Comparative Example 1 The technical solution of Example 2 is adopted, but the differences are: 1) the addition of Sophora flavescens is omitted in the step of preparing the composite enzymatic hydrolysate, and Poria cocos is used to make up the weight of Sophora flavescens; 2) the addition of Inula japonica is omitted in the step of preparing the extract, and Licorice is used to make up the weight of Inula japonica.

[0089] Comparative Example 2 The technical solution of Example 2 is adopted, but the differences are as follows: 1) in the step of preparing the pre-composite carrier, the subsequent use of ferrous chloride tetrahydrate, zinc chloride monohydrate, 2,2'-bipyrimidine, potassium oxalate, etc. is omitted, and the pretreated carrier is used instead of the pre-composite carrier in the subsequent carrier modification step; 2) in the mixed preparation step, the addition of Bifidobacterium lactis powder and Lactobacillus plantarum powder is omitted.

[0090] Comparative Example 3 The technical solution of Example 2 is adopted, but the differences are as follows: 1) in the step of preparing the pre-composite carrier, the treatment of defatted rice bran and defatted bone meal by the pretreatment liquid is omitted, and defatted rice bran and defatted bone meal are used in the subsequent treatment in a weight ratio of 5.5:1.4 instead of the pretreatment carrier; 2) the carrier modification step is omitted.

[0091] Test Example 1 The health feed additives for enhancing animal immunity in Examples 1-3 and Comparative Examples 1-3 were used to conduct a broiler feeding test. Specifically, a broiler farm in Anqiu, Weifang City was selected as the test site, and 140 healthy white-feathered broilers of 7 days of age and similar weight were selected and randomly divided into 7 groups, and the broilers in each group were numbered, weighed and calculated, and the average weight was recorded as the broiler weight on the 0th day of feeding.

[0092] Among them, groups 1-6 were fed with the health-care feed additives for enhancing animal immunity of Examples 1-3 and Comparative Examples 1-3 in combination with conventional broiler feed (the amount of feed additives added was 2% of the weight of conventional feed); Group 7 was fed only with conventional broiler feed as a blank control group. Each group drank water normally during the feeding process. On the 7th, 14th and 21st days of feeding, the broilers in each group were weighed, and the mean weight was calculated, which was recorded as the average weight of the broilers on the 7th, 14th and 21st days of feeding.

[0093] The composition and nutritional level of the conventional broiler feed used in Experimental Example 1 are shown in the following table:

[0094] On the 0th, 7th, 14th and 21st day of feeding, the average body weight of broilers in each group is shown in the following table:

[0095] Furthermore, during the feeding process of the broiler chickens in the aforementioned groups 1-7, blood was collected from the jugular vein of the broiler chickens in each group on the 14th, 21st and 29th days of feeding, and the serum was separated and tested for Newcastle disease antibodies, and the average value was taken. The specific results are shown in the following table:

[0096] It can be seen that compared with Comparative Example 1 which only uses Poria cocos hydrolysate and licorice extract, the feed additives of Examples 1-3 of the present invention are prepared by extracting and hydrolyzing Poria cocos and Sophora flavescens to obtain a composite hydrolysate; the composite extract is prepared by extracting and treating Inula officinalis and licorice with alcohol and water; the effective active ingredients in the composite hydrolysate and the composite extract are combined with probiotics to effectively promote the growth and development of broilers, increase the weight of broilers, and simultaneously improve the immunity and antiviral ability of broilers. After Comparative Example 2 omits the subsequent composite treatment of the pre-treated carrier, the binding performance of the carrier with the composite hydrolysate and the effective active ingredients in the composite extract is reduced, the anti-interference performance of the feed additive during use is reduced, and it lacks effective coordination with probiotics, which is specifically manifested in that the weight of the broilers in Comparative Example 2 is reduced compared with Example 2, and the improvement effect on the immunity and antiviral ability of broilers is reduced. In Comparative Example 3, the treatment of defatted rice bran and defatted bone meal with the pretreatment solution containing erucic acid amide propyl trimethyl ammonium chloride was omitted, and the binding stability of defatted rice bran, defatted bone meal with the metal organic framework material was reduced. At the same time, the modification treatment of the pre-composite carrier with N,N,N-trimethylglycine was omitted, and the carrier could not effectively combine with the effective active ingredients in the composite enzymatic hydrolyzate and the composite extract. The plant active ingredients were lost and failed during their preparation, use and storage, which led to a reduction in the effect of the feed additive. Specifically, the weight of the broilers in Comparative Example 3 was significantly reduced compared with that in Example 2, and the improvement effect on the immunity and antiviral ability of broilers was significantly reduced.

[0097] Furthermore, on the 14th day of feeding the broilers in the aforementioned groups 1-7, the brightness of the feathers on the head and back of the broilers in each group was detected using a CR-400 colorimeter, and the L value in the colorimeter was taken as the brightness value. The specific results are shown in the following table:

[0098] Furthermore, during the 14th to 22nd day of feeding the broiler chickens in the aforementioned groups 1-7, the average daily feed intake of the broiler chickens in each group and the diarrhea rate of the broiler chickens were counted. The specific results are shown in the following table:

[0099] It can be seen that the broilers fed with the feed additives of Examples 1-3 had no significant change in the average daily feed intake from the 14th day to the 22nd day compared with the blank control group, and the feed additives of Examples 1-3 had good palatability and would not affect the normal eating of the broilers; at the same time, the feed additives of Examples 1-3 would not irritate the stomach and intestines of the broilers, and could effectively prevent diarrhea in the broilers, and could also regulate the intestines of the broilers, and solve problems with animal eating, diarrhea, etc.; and the feed additives of Examples 1-3 could also increase the glossiness of the broiler feathers and regulate the physique of the broilers.

[0100] Test Example 2 The health feed additives for enhancing animal immunity in Examples 1-3 and Comparative Examples 1-3 were used to conduct a feeding test for pet dogs. Specifically, a kennel in Jinan was selected as the test site, and 70 healthy pet dogs of 2 months old and similar weight were selected and randomly divided into 7 groups.

[0101] Among them, groups 1-6 were fed with the health-care feed additives for enhancing animal immunity of Examples 1-3 and Comparative Examples 1-3 in combination with conventional dog food (the amount of feed additives added was 3% of the weight of conventional feed); group 7 was fed with conventional dog food only as a blank control group. During the feeding process, each group drank water normally. The feeding time was 30 days.

[0102] After 30 days of feeding, blood samples were collected from the pet dogs in each group to test the levels of immune proteins IgA, IgG, and IgM in each pet dog. The specific results are shown in the following table:

[0103] Furthermore, when the pet dogs in the aforementioned groups 1-7 were fed for 25 days, the fresh feces of the pet dogs in each group were collected and refrigerated, and then within 2 hours of feces collection, 1.0g of feces were weighed in a sterile environment and placed in a sterile test tube, 9mL of sterile saline was added, and after sufficient shaking and dilution, the samples were spread on agar medium for aerobic culture for 24 hours and then the colonies were counted; and the samples were spread on MRS agar medium for anaerobically cultured for 48 hours and then the colonies were counted. The specific results are shown in the following table:

[0104] Furthermore, when the pet dogs in the aforementioned groups 1-3 and group 7 (blank control group) were fed for 28 days, the brightness and softness of the coat of the pet dogs in each group were visually observed; it was observed that the coat of the pet dogs fed with the feed additives of Examples 1-3 was shiny, moist and soft, and its coat brightness and softness were significantly better than those of the blank control group.

[0105] It can be seen that compared with Comparative Example 1 which only uses Poria cocos hydrolysate and licorice extract, the feed additives of Examples 1-3 of the present invention are prepared by extracting and enzymolyzing Poria cocos and Sophora flavescens to obtain a composite enzymolyte; and by subjecting Inula officinalis and licorice to an alcohol-water mixed extraction treatment to obtain a composite extract; the effective active ingredients in the composite enzymolyte and the composite extract are combined with probiotics to effectively improve the immunity of pet dogs, improve the intestinal environment of pet dogs, increase the content of beneficial bacteria (Lactobacillus, Bifidobacterium) in the intestine, reduce the content of harmful bacteria (Escherichia coli), and improve the coat quality of pet dogs. After the subsequent composite treatment of the pretreated carrier was omitted in Comparative Example 2, the binding performance of the carrier with the effective active ingredients in the composite enzymatic hydrolysate and the composite extract was reduced, the anti-interference performance of the feed additive during use was reduced, and it lacked effective cooperation with the probiotics, which was specifically manifested in that the content of immune proteins IgA, IgG, and IgM in the pet dogs of Comparative Example 2 was lower than that in Example 2, and the effect of improving the immunity of the pet dogs was reduced; at the same time, since the addition of probiotics was omitted in Comparative Example 2, the bacterial content of each bacteria in the intestinal flora of the pet dogs decreased, but it was still significantly better than the blank control group. Analysis showed that the plant active ingredients in the composite enzymatic hydrolysate and the composite extract had an improvement effect on the gastrointestinal conditioning of the pet dogs. In Comparative Example 3, the treatment of defatted rice bran and defatted bone meal with the pretreatment solution containing erucic acid amide propyl trimethyl ammonium chloride is omitted, and the binding stability of defatted rice bran, defatted bone meal with the metal organic framework material is reduced. At the same time, the modification treatment of the pre-composite carrier with N,N,N-trimethylglycine is omitted, and the carrier cannot be effectively combined with the effective active ingredients in the composite enzymatic hydrolyzate and the composite extract. The plant active ingredients are lost and lose their effectiveness during their preparation, use and storage, which leads to a reduction in the effect of the feed additive. Specifically, the content of immune proteins IgA, IgG, and IgM in the pet dogs of Comparative Example 3 is significantly reduced compared with those in Example 2, and the effect of modifying the immunity of the pet dogs is reduced; and the conditioning effect on the gastrointestinal tract of the pet dogs is reduced, which is specifically manifested in that the content of beneficial bacteria in the feces of the pet dogs is reduced compared with that in Example 2, and the content of harmful bacteria is increased compared with that in Example 2.

[0106] Test Example 3 The feed additives of Example 2, Comparative Example 2 and Comparative Example 3 were placed in an environment with a temperature of 45°C and a relative humidity of 80%. After standing for 120 days, the feed additives of Example 2, Comparative Example 2 and Comparative Example 3 were used for feeding pet dogs according to the test method of Test Example 2. After 30 days of feeding, blood was collected from the pet dogs in each group to detect the content of immune proteins IgA, IgG and IgM in each pet dog. The specific results are shown in the following table:

[0107] It can be seen that the feed additive of the present invention has good storage resistance. After being stored in a high temperature and humid environment, it can be used for feeding pet dogs. The effect of improving the immunity of pet dogs is not significantly different from that before storage, and the feed additive can maintain its due performance for a long time. After the subsequent composite treatment of the pretreated carrier was omitted in Comparative Example 2, the long-term stability of the combination of the carrier with the effective active ingredients in the composite enzymatic hydrolysate and the composite extract was reduced, and the effective ingredients were easily lost and ineffective during high-temperature and humid storage, and the immunity improvement effect on pet dogs was reduced; at the same time, Comparative Example 3 omitted the treatment of defatted rice bran and defatted bone meal with the pretreatment solution containing erucic acid amide propyl trimethyl ammonium chloride, and the binding stability of defatted rice bran, defatted bone meal and metal organic framework materials was reduced. At the same time, the modification treatment of the pre-composite carrier by N, N, N-trimethylglycine was omitted, and the carrier could not be effectively combined with the effective active ingredients in the composite enzymatic hydrolysate and the composite extract. The plant active ingredients were lost and ineffective during their preparation, use and storage, which directly led to a significant reduction in the application effect of the feed additive after long-term storage in a high-temperature and humid environment, and a reduction in the immunity improvement effect on pet dogs; specifically, the content of immune proteins IgA, IgG, and IgM in pet dogs fed with the feed additives of Comparative Examples 2 and Comparative Examples 3 was reduced.

[0108] In summary, the preparation method of the health-care feed additive for enhancing animal immunity of the present invention comprises the following steps: extracting and precipitating Poria cocos and Sophora flavescens with sodium hydroxide solution, and then enzymolyzing the precipitate with papain and trypsin to obtain a composite enzymolysis solution; extracting and concentrating Inula and Licorice with alcohol and water to obtain a composite extract solution; and then, in the step of preparing a pre-composite carrier, selecting defatted rice bran and defatted bone meal as carrier matrix materials, and treating them with a pre-treatment solution containing erucamidopropyl trimethyl ammonium chloride, and adjusting the defatted rice bran and defatted bone meal by erucamidopropyl trimethyl ammonium chloride. The surface charge distribution of fat-bone meal and the modification of its surface improve the binding stability between the pre-treated carrier and the iron / zinc-based composite organic metal framework material, improve the storage resistance of the feed additive, and enable the feed additive to maintain its original performance after long-term storage; the pre-treated carrier is combined with the iron / zinc-based composite organic metal framework material in an in-situ composite manner to obtain a pre-composite carrier; the large specific surface area and multi-porous characteristics of the composite carrier can effectively and stably combine with the plant active ingredients, thereby achieving effective protection of the plant active ingredients, improving the anti-interference and antioxidant capabilities of the plant active ingredients, and avoiding the active ingredients in The feed additive is ineffective during the processing, preparation and storage process, so as to avoid the ineffectiveness caused by other ingredients in animal feed, and to supplement the nutrients in feed additives, thereby improving the utilization rate of effective ingredients in feed additives; at the same time, the pre-composite carrier can also improve the absorption of effective ingredients in the animal stomach and intestine; then in the carrier modification step, N,N,N-trimethylglycine is used to modify the pre-composite carrier, so as to further improve the binding performance of the modified carrier with the effective ingredients in the composite enzymatic hydrolysate and composite extract, and to achieve effective coordination of various plant ingredients, and to improve gastrointestinal digestion and absorption, thereby further improving the immunity of animals. ; Finally, in the mixed preparation step, a composite plant component containing effective ingredients of Poria cocos, Sophora flavescens, Inula japonica and Licorice is used in combination with Bifidobacterium lactis powder and Lactobacillus plantarum powder to prepare a health-care feed additive for enhancing animal immunity. Various technical means cooperate with each other and work synergistically, which can effectively reduce the amount of antibiotics used in the animal breeding process. A variety of plant components can be stably coordinated with other raw materials to improve animal immunity while maintaining health and conditioning the animal body, and the utilization rate of plant components is high; the stability, anti-interference and antioxidant properties of the plant components in the feed additive are improved, and the storage resistance of the feed additive is improved.

[0109] Unless otherwise specified, all percentages used in the present invention are by mass.

[0110] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing a health-care feed additive for enhancing animal immunity, characterized in that: The method comprises the following steps: preparing a composite enzymatic hydrolysate, preparing a composite extract, preparing a pre-composite carrier, modifying the carrier, and preparing a mixed preparation; The method for preparing the composite enzymatic hydrolysate comprises the following steps: respectively crushing dried Poria cocos and Sophora flavescens into Poria cocos powder and Sophora flavescens powder; extracting the Poria cocos powder and Sophora flavescens powder with a sodium hydroxide solution, precipitating, and separating to obtain a solid; enzymatically hydrolyzing the solid with papain and trypsin, and then inactivating the enzymes to obtain the composite enzymatic hydrolysate; The method for preparing the composite extract comprises: crushing dried inulin and licorice into inulin powder and licorice powder respectively; extracting the inulin powder and licorice powder with ethanol solution, and concentrating the extract to obtain the composite extract; The method for preparing the pre-composite carrier comprises the following steps: adding defatted rice bran and defatted bone meal into a pre-treatment liquid, heating the liquid to 40-45° C., keeping the temperature and stirring, separating and obtaining a solid, washing and drying the solid, and obtaining the pre-treatment carrier; then adding the pre-treatment carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, and 2,2'-bipyrimidine into a methanol solution, mixing the mixture evenly, adding potassium oxalate, keeping the temperature and stirring at 42-45° C., separating and obtaining a solid, washing and drying the solid, and obtaining the pre-composite carrier; The pretreatment liquid is an isopropanol aqueous solution in which erucamidopropyltrimethylammonium chloride is dispersed; The carrier modification method comprises: performing carrier modification treatment on the pre-composite carrier using N,N,N-trimethylglycine in a solvent environment to obtain a modified carrier; The mixed preparation method comprises the following steps: evenly mixing the composite enzymatic hydrolysate and the composite extract, adding the modified carrier, stirring at room temperature, separating the solid matter, and drying to obtain the composite plant component; evenly mixing the composite plant component, Bifidobacterium lactis powder, and Lactobacillus plantarum powder to obtain the health feed additive capable of enhancing animal immunity.

2. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the preparation of the pre-composite carrier, the defatted rice bran and defatted bone meal are kept in the pre-treatment liquid and stirred for 17-19 hours; The weight ratio of defatted rice bran, defatted bone meal and pretreatment liquid is 5-6:1.3-1.5:80-85; In the pretreatment liquid, the weight percentage content of erucamide propyltrimethylammonium chloride is 1.6-1.8wt%, and the volume concentration of the isopropanol aqueous solution is 32-34%.

3. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the preparation of the pre-composite carrier, the stirring time at 42-45° C. is 10-12 hours; The weight ratio of the pretreated carrier, ferrous chloride tetrahydrate, zinc chloride monohydrate, 2 2'-bipyrimidine, methanol solution, and potassium oxalate is 16-16.5:2-2.2:0.7-0.8:3.6-4.1:95-100:3.2-3.3; The volume concentration of the methanol solution is 50-55%.

4. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: The carrier modification method comprises the following steps: adding N,N,N-trimethylglycine to an ethanol solution, mixing uniformly, adding a pre-composite carrier, heating to 35-40° C., stirring for 5-6 hours, separating and obtaining a solid, washing and drying the solid, and obtaining a modified carrier.

5. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the carrier modification, the solvent is an ethanol solution with a volume concentration of 30-35%; The weight ratio of N,N,N-trimethylglycine, solvent and pre-composite carrier is 2-2.2:100:10-11.

6. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the mixed preparation, the stirring time at room temperature after adding the modified carrier is 8-10 hours; The weight ratio of the composite enzymatic hydrolysate, the composite extract and the modified carrier is 30-35:65-70:18-20; The weight ratio of the composite plant component, the lactobacillus powder and the plant lactobacillus powder is 100:3.1-3.2:1.9-2.

7. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the preparation of the composite enzymatic hydrolysate, the mass concentration of the sodium hydroxide solution is 0.04-0.05wt%; The temperature of extracting Poria cocos powder and Sophora flavescens powder with sodium hydroxide solution is 35-40℃, and the extraction time is 1.5-2.5h; The weight ratio of Poria cocos powder, Sophora flavescens powder and sodium hydroxide solution is 0.7-0.8:1.5-1.6:18-20.

8. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the preparation of the composite enzymatic hydrolysate, the enzymatic hydrolysis temperature is 40-45° C. and the enzymatic hydrolysis time is 8-10 h; The weight ratio of solid matter, papain and trypsin is 100-105:3-4:1.2-1.

6.

9. The method for preparing the health-care feed additive for enhancing animal immunity according to claim 1, characterized in that: In the preparation of the composite extract, the extraction temperature is 80-85° C. and the extraction time is 2-2.5 h; The weight ratio of the micropowder of Inula officinalis, the micropowder of Licorice and the ethanol solution is 2-2.2:4.5-4.6:115-120; The volume concentration of the ethanol solution is 65-70%.

10. A health feed additive for enhancing animal immunity, characterized in that: The method is prepared by the method according to any one of claims 1 to 9.

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