Anti-aging composition liposome, preparation method thereof and cosmetics

Abstract

The invention provides an anti-aging composition liposome, a preparation method thereof and cosmetics, and relates to the technical field of biomedical materials. The anti-aging composition liposome comprises betaine, xylitol, short-chain fatty alcohol, phospholipid, an antioxidant and an anti-aging composition, the anti-aging composition is prepared from CutiFine CLR, CutiGuard CLR, a cell lysis product of a fermentation product of a bifidus yeast and oleuropein. According to the anti-aging composition lipidosome provided by the invention, the betaine and the xylitol are adopted to form a deep eutectic solvent, so that the anti-aging composition lipidosome has a relatively good infiltration promotion effect, and the stability of the anti-aging composition is improved by carrying out synergistic infiltration promotion in combination with a lipidosome technology. The anti-aging composition lipidosome improves the skin residence rate, effectively improves the local treatment effect, reduces potential side effects, and enhances the stability and transdermal absorption of active ingredients, thereby playing an important role in skin care and anti-aging treatment.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical materials, and in particular to an anti-aging composition liposome, a preparation method thereof and cosmetics. Background Art

[0002] The anti-aging composition includes CutiFine CLR, CutiGuard CLR, bifid yeast fermentation lysate and oleuropein. As a complex, it has relatively complex ingredients and poor stability. It will settle after standing for a long time. Moreover, since most of them are extracts or fermentation products with strong polarity, it is difficult for them to penetrate the stratum corneum of the skin to exert their effects.

[0003] Liposomes are a type of inclusion body formed by self-assembly of phosphatidylcholine. They can carry both water-soluble and fat-soluble components, so the water-soluble components in the anti-aging composition can be loaded in its center. The structure of liposomes is very similar to that of cell membranes, and they are highly biocompatible and biodegradable, so they can carry the anti-aging composition through the stratum corneum of the skin to exert its effects.

[0004] A deep eutectic solvent is a solvent formed by a hydrogen bond acceptor and a hydrogen bond donor. It has similar properties to ionic liquids that promote transdermal absorption, and therefore can be used as a good transdermal penetration enhancer. Betaine is commonly used in cosmetics as an anti-inflammatory and anti-aging substance. It is also a strong hydrogen bond acceptor that can form a deep eutectic solvent with a variety of hydrogen bond donors. It mainly enhances the transdermal penetration of drugs by extracting lipids from the stratum corneum or changing the conformation of keratin. Existing technologies use deep eutectic solvents to promote transdermal penetration of drugs. Most of them promote the transdermal absorption of a single component or a small molecular weight component. There is no more research on the transdermal absorption of two or more targets.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] One of the purposes of the present invention is to provide an anti-aging composition liposome to solve at least one of the above technical problems in the prior art.

[0007] The second object of the present invention is to provide a method for preparing the anti-aging composition liposome.

[0008] A third object of the present invention is to provide a cosmetic.

[0009] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0010] The first aspect of the present invention provides an anti-aging composition liposome, comprising betaine, xylitol, short-chain fatty alcohol, phospholipids, antioxidants and an anti-aging composition;

[0011] The anti-aging composition includes CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein.

[0012] Furthermore, in the anti-aging composition, the mass ratio of CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein is (0.5-3):(1-8):(0.5-3):(0.01-0.1), preferably 1:5:1:0.01-0.1.

[0013] Furthermore, the preparation method of the anti-aging composition is to uniformly mix CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein to obtain the anti-aging composition.

[0014] Furthermore, the anti-aging composition liposome comprises, by mass percentage, 0.3-5% betaine, 0.3-5% xylitol, 50-70% short-chain fatty alcohol, 3-8% phospholipids, 0.1-0.5% antioxidant and 10-45% anti-aging composition.

[0015] Furthermore, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propylene glycol, 1,3-propylene glycol, glycerol, butylene glycol, pentanediol, and hexanediol, preferably 1,3-propylene glycol and glycerol.

[0016] Further, the phospholipids include soybean lecithin and / or hydrogenated lecithin;

[0017] Preferably, the antioxidant includes sodium metabisulfite, sodium thiosulfate, L-cysteine ​​and glutathione.

[0018] The second aspect of the present invention provides a method for preparing the anti-aging composition liposome, comprising the following steps:

[0019] A. Evenly mix betaine, xylitol and some short-chain fatty alcohols, and then add phospholipids thereto and mix evenly to obtain a mixed solution;

[0020] B. Evenly mix the remaining short-chain fatty alcohol, antioxidant and anti-aging composition, add them into the mixed solution to obtain a uniform solution, and finally homogenize the uniform solution to obtain the anti-aging composition liposome.

[0021] Furthermore, in step A, the mixing temperature is 50-80°C.

[0022] Preferably, it is added to the mixed solution under stirring.

[0023] Preferably, the stirring speed is 300-400 rpm and the stirring time is 5-15 min.

[0024] Furthermore, the homogenization pressure is 15000-25000 psi.

[0025] Preferably, the homogenization cycle is performed 1 to 5 times.

[0026] Preferably, the discharge temperature after homogenization is 10-30°C.

[0027] The third aspect of the present invention provides a cosmetic comprising the anti-aging composition liposomes described in the first aspect.

[0028] Compared with the prior art, the present invention has at least the following beneficial effects:

[0029] The anti-aging composition liposome provided by the present invention adopts betaine and xylitol to form a deep eutectic solvent, has a good penetration-promoting effect, and is synergistically promoted by combining liposome technology. The water-soluble substance in the anti-aging composition is dispersed in the hydrophilic center of the liposome, and the fat-soluble substance is dispersed in the liposome membrane layer. The presence of the liposome greatly improves the stability of the anti-aging composition. In vitro transdermal experiments show that the skin retention of the anti-aging composition liposome is increased by about 7 times, and the anti-aging composition liposome improves the skin retention rate, effectively improves the local treatment effect, reduces potential side effects, and enhances the stability and transdermal absorption of the active ingredients, thereby playing an important role in skin care and anti-aging treatment.

[0030] The method for preparing the anti-aging composition liposome provided by the present invention comprises the following steps: in the process of preparing the mixed solution, betaine and xylitol form a deep eutectic solvent, and then the liposome preparation technology is performed, the two processes are combined, and the solubility, stability and later bioavailability of the liposome are improved.

[0031] The cosmetics provided by the present invention, in view of the advantages of the above-mentioned anti-aging composition liposomes, enable the prepared cosmetics to enhance the anti-aging effect, and also improve the safety, stability and transdermal absorption capacity of the product, thereby providing consumers with an anti-aging option that is efficient, safe and applicable to a wide range of people. DETAILED DESCRIPTION

[0032] The embodiments of the present invention will be described in detail below in conjunction with the embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only used to illustrate the present invention and should not be considered to limit the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0033] The first aspect of the present invention provides an anti-aging composition liposome, comprising betaine, xylitol, short-chain fatty alcohol, phospholipids, antioxidants and an anti-aging composition;

[0034] The anti-aging composition includes CutiFine CLR, CutiGuard CLR, bifida yeast fermentation product lysate and oleuropein.

[0035] In the anti-aging composition, CutiFine CLR acts on the dermal compartment around the hair follicles to enhance the structural support of the skin, thereby maintaining small pores; CutiGuard CLR is a cell aging reversal factor that prevents premature skin aging by reducing the number of senescent cells and related inflammation and destructive mediators that cause obvious skin aging, while also reducing wrinkles; the lysate of the bifid yeast fermentation product promotes precise DNA repair and prevents DNA damage caused by ultraviolet radiation, and has the function of protecting the surface of the skin; oleuropein also has the function of protecting cells from ultraviolet damage and preventing ultraviolet rays from decomposing the skin membrane. Therefore, the bifid yeast fermentation product lysate and oleuropein in the anti-aging composition serve as the first line of defense for skin anti-aging, preventing cell aging by avoiding ultraviolet damage; CutiFine CLR and CutiGuardCLR perform anti-aging effects on cells at a deeper level through repair, anti-inflammatory, and anti-oxidation. These substances jointly promote skin anti-aging and anti-aging, achieving better anti-aging effects.

[0036] The main active ingredients of CutiFine CLR are Vaccaria segetalis extract and Baobab fruit pulp extract. Vaccaria segetalis extract contains saponins, flavonoids, polysaccharides and cyclic peptides, which have anti-inflammatory, antioxidant, anti-angiogenesis and even anti-tumor effects; Baobab fruit pulp extract mainly contains high content of calcium and vitamin C, in addition to carbohydrates, triterpenes, saponins and riboflavin.

[0037] CutiFine CLR can improve the problem of obvious skin pores by reducing skin secretion, reducing glycation, scavenging free radicals, reducing inflammation, and strengthening the dermis structure.

[0038] CutiGuard CLR is a cell aging reversal factor. Its main active ingredients are betaine and hydrolyzed red algae extract, which can prevent premature skin aging by reducing the number of senescent cells and the related inflammation and destructive mediators that lead to obvious skin aging, while also reducing wrinkles.

[0039] The lysate of the bifid yeast fermentation product can promote precise DNA repair and prevent DNA damage caused by ultraviolet radiation, and has the function of protecting the surface of the skin; oleuropein also protects cells from damage by ultraviolet rays and prevents ultraviolet rays from decomposing the skin membrane. Therefore, the bifid yeast fermentation product lysate and oleuropein in the anti-aging composition serve as the first line of defense for skin anti-aging, preventing cell aging by avoiding damage from ultraviolet rays; CutiFineCLR and CutiGuard CLR perform anti-aging effects on cells at a deeper level through repair, anti-inflammatory, and anti-oxidation, and they can jointly promote skin anti-aging and anti-aging.

[0040] Furthermore, the mass ratio of CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein is (0.5-3):(1-8):(0.5-3):(0.01-0.1), preferably 1:5:1:0.01-0.1.

[0041] Typically but not limiting, the mass ratio of CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein can be 0.5:1:0.5:0.01, 1:2:1:0.02, 2:6:1.5:0.05, 3:8:3:0.1, or any other ratio in the range of (0.5-3):(1-8):(0.5-3):(0.01-0.1); the preferred ratio range can be 1:5:1:0.01, 1:5:1:0.02, 1:5:1:0.03, 1:5:1:0.04, 1:5:1:0.05, 1:5:1:0.06, 1:5:1:0.07, 1:5:1:0.08 or 1:5:1:0.09, or any value in the range of 1:5:1:0.01-0.1.

[0042] The preparation method of the anti-aging composition is to uniformly mix CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein to obtain the anti-aging composition.

[0043] The preparation method of the anti-aging composition provided by the present invention can obtain the anti-aging composition by uniformly mixing all raw materials, has a simple process, a large batch processing volume, and is suitable for industrial large-scale production.

[0044] Anti-aging compositions contain a variety of effective substances, which have complex ingredients, poor stability, strong hydrophilicity, and are not easily absorbed by the human stratum corneum. Therefore, liposome technology is used to embed water-soluble ingredients in the center of the liposome, which can both improve stability and increase transdermal absorption capacity.

[0045] The anti-aging composition liposome provided by the present invention disperses the water-soluble substances in the anti-aging composition in the hydrophilic center of the liposome, and the fat-soluble substances are dispersed in the liposome membrane layer. The presence of the liposome greatly improves the stability of the anti-aging composition. In vitro transdermal experiments show that the skin retention of the anti-aging composition liposome is increased by about 7 times, and the anti-aging composition liposome increases the skin retention rate, effectively improves the local treatment effect, reduces potential side effects, and enhances the stability and transdermal absorption of the active ingredients, thereby playing an important role in skin care and anti-aging treatment.

[0046] Furthermore, the anti-aging composition liposome comprises, by mass percentage, 0.3-5% betaine, 0.3-5% xylitol, 50-70% short-chain fatty alcohol, 3-8% phospholipids, 0.1-0.5% antioxidant and 10-45% anti-aging composition.

[0047] Typically but not limiting, the mass percentage of betaine in the anti-aging composition liposome can be 0.3%, 0.5%, 1%, 2%, 3%, 4% or 5%, or any value within the range of 0.3% to 5%; the mass percentage of xylitol can be 0.3%, 0.5%, 1%, 2%, 3%, 4% or 5%, or any value within the range of 0.3% to 5%; the mass percentage of short-chain fatty alcohol can be 50%, 52%, 54%, 56%, 58%, 60%, 62%, 64%, 66%, 68% or 70%, or any value within the range of 50% to 70%; the mass percentage of phospholipids can be 0.5%, 1%, 2%, 3%, 4% or 5%, or any value within the range of 0.3% to 5%. The mass percentage of the antioxidant can be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%, or any value within the range of 0.1% to 0.5%; the mass percentage of the anti-aging composition can be 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40% or 45%, or any value within the range of 10% to 45%.

[0048] Furthermore, the short-chain fatty alcohol includes at least one of ethanol, 1,2-propylene glycol, 1,3-propylene glycol, glycerol, butylene glycol, pentanediol, and hexanediol, preferably 1,3-propylene glycol and glycerol.

[0049] Preferably, the phospholipids include soy lecithin and / or hydrogenated lecithin.

[0050] Preferably, the antioxidant includes sodium metabisulfite, sodium thiosulfate, L-cysteine ​​and glutathione.

[0051] The second aspect of the present invention provides a method for preparing the anti-aging composition liposome, comprising the following steps:

[0052] A. Evenly mix betaine, xylitol and some short-chain fatty alcohols, and then add phospholipids thereto and mix evenly to obtain a mixed solution;

[0053] B. Evenly mix the remaining short-chain fatty alcohol, antioxidant and anti-aging composition, add them into the mixed solution to obtain a uniform solution, and finally homogenize the uniform solution to obtain the anti-aging composition liposome.

[0054] The method for preparing the anti-aging composition liposome provided by the present invention comprises the following steps: in the process of preparing the mixed solution, betaine and xylitol form a deep eutectic solvent, and then the liposome preparation technology is performed, and the two processes are combined to improve the solubility, stability and later bioavailability of the liposome. Because the presence of the deep eutectic solvent changes the structure of the stratum corneum cells, it helps the fusion of the liposome particles with the keratin lipids, thereby assisting the transfer of the active substance to the dermis, promoting the penetration of the liposome, and thus improving the anti-aging effect of the liposome.

[0055] Liposomes are a type of inclusion body formed by self-assembly of phosphatidylcholine. They can carry both water-soluble and fat-soluble components, so the water-soluble components in the anti-aging composition can be loaded in its center. The structure of liposomes is very similar to that of cell membranes, and they are highly biocompatible and biodegradable, so they can carry the anti-aging composition through the stratum corneum of the skin to exert its effects.

[0056] A deep eutectic solvent is a solvent formed by a hydrogen bond acceptor and a hydrogen bond donor. It has similar properties to ionic liquids that promote transdermal absorption, and therefore can be used as a good transdermal penetration enhancer. Betaine is commonly used in cosmetics as an anti-inflammatory and anti-aging substance. It is also a strong hydrogen bond acceptor that can form a deep eutectic solvent with a variety of hydrogen bond donors. It mainly enhances the transdermal penetration of drugs by extracting lipids from the stratum corneum or changing the conformation of keratin. The present invention combines liposome technology for synergistic penetration enhancement.

[0057] Furthermore, in step A, the mixing temperature is 50-80°C.

[0058] Typically but not limiting, the mixing temperature in step A can be, for example, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C or 80°C, or any value within the range of 50°C to 80°C.

[0059] Preferably, it is added to the mixed solution under stirring.

[0060] Preferably, the stirring speed is 300-400 rpm and the stirring time is 5-15 min.

[0061] Typically but not limitatively, the stirring speed may be, for example, 300 rpm, 320 rpm, 340 rpm, 360 rpm, 380 rpm, 400 rpm, or any value within the range of 300-400 rpm; the stirring time may be, for example, 5 min, 7 min, 9 min, 11 min, 13 min, 15 min, or any value within the range of 5-15 min.

[0062] Furthermore, the homogenization pressure is 15000-25000 psi.

[0063] Typically but not limiting, the homogenization pressure may be, for example, 15,000 psi, 18,000 psi, 21,000 psi, 24,000 psi, 27,000 psi, or any value within the range of 15,000 to 25,000 psi.

[0064] Preferably, the homogenization cycle is performed 1 to 5 times.

[0065] Typically but not limiting, the number of homogenization cycles may be, for example, 1, 2, 3, 4, 5 times, or any value within the range of 1 to 5 times.

[0066] Preferably, the discharge temperature after homogenization is 10-30°C.

[0067] Typically but not limiting, the discharge temperature after homogenization may be, for example, 10°C, 15°C, 20°C, 25°C, 30°C, or any value within the range of 10-30°C.

[0068] The third aspect of the present invention provides a cosmetic comprising the anti-aging composition liposome.

[0069] The cosmetics provided by the present invention, in view of the advantages of the above-mentioned anti-aging composition liposomes, enable the prepared cosmetics to enhance the anti-aging effect, and also improve the safety, stability and transdermal absorption capacity of the product, thereby providing consumers with an anti-aging option that is efficient, safe and applicable to a wide range of people.

[0070] The present invention is further described below by specific examples and comparative examples, but it should be understood that these examples are only used for more detailed description and should not be understood as limiting the present invention in any form. The raw materials used in the examples and comparative examples of the present invention, if no specific conditions are specified, are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used without indicating the manufacturer are all conventional products that can be purchased commercially.

[0071] Example 1

[0072] This embodiment provides an anti-aging composition, which is obtained by uniformly mixing CutiFine CLR (CLRBerlin, Germany), CutiGuard CLR (CLR Berlin, Germany), bifid yeast fermentation product lysate and oleuropein in a mass ratio of 1:5:1:0.05.

[0073] Example 2

[0074] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0075] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 0.9%, soy lecithin is 5%, glycerol is 38.3%, the anti-aging composition of Example 1 is 35.2%, and sodium pyrosulfite is 0.1%.

[0076] The specific preparation method is as follows:

[0077] 1. Stir betaine, xylitol and 1,3-propylene glycol at 50-80°C until the solution becomes transparent, then add soy lecithin, stir at 65°C until dissolved and transparent, then cool to 50°C to obtain a mixed solution.

[0078] 2. Stir glycerol, the anti-aging composition and sodium metabisulfite until they are completely mixed without particles, and then slowly add them into the mixed solution at a speed of 400 rpm for 10 minutes to obtain a uniform solution.

[0079] 3. The uniform solution was homogenized by a homogenizer, the homogenization pressure was controlled to be 20000 psi, the number of cycles was 3 times, and the discharge temperature was 20° C., and the anti-aging composition liposome was obtained.

[0080] Example 3

[0081] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0082] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 0.3%, soy lecithin is 5%, glycerol is 38.9%, the anti-aging composition of Example 1 is 35.2%, and sodium pyrosulfite is 0.1%.

[0083] The preparation method is the same as that of Example 2.

[0084] Example 4

[0085] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0086] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 5%, soy lecithin is 5%, glycerol is 34.2%, the anti-aging composition of Example 1 is 35.2%, and sodium pyrosulfite is 0.1%.

[0087] The preparation method is the same as that of Example 2.

[0088] Example 5

[0089] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0090] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 0.9%, soy lecithin is 5%, glycerol is 63.5%, the anti-aging composition of Example 1 is 10%, and sodium pyrosulfite is 0.1%.

[0091] The preparation method is the same as that of Example 2.

[0092] Example 6

[0093] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0094] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 0.9%, soy lecithin is 5%, glycerol is 28.5%, the anti-aging composition of Example 1 is 45%, and sodium pyrosulfite is 0.1%.

[0095] The preparation method is the same as that of Example 2.

[0096] Example 7

[0097] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0098] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 0.9%, soy lecithin is 5%, glycerol is 64.5%, the anti-aging composition of Example 1 is 9%, and sodium pyrosulfite is 0.1%.

[0099] The preparation method is the same as that of Example 2.

[0100] Example 8

[0101] This embodiment provides an anti-aging composition liposome, which is composed of the following components:

[0102] 1,3-Propanediol is 20%, betaine is 0.5%, xylitol is 0.9%, soy lecithin is 5%, glycerol is 27.5%, the anti-aging composition of Example 1 is 46%, and sodium pyrosulfite is 0.1%.

[0103] The preparation method is the same as that of Example 2.

[0104] Comparative Example 1

[0105] This comparative example provides an anti-aging composition emulsion, which is composed of the following components:

[0106] The content of 1,3-propylene glycol was 20%, the content of glycerol was 44.7%, the content of the anti-aging composition of Example 1 was 35.2%, and the content of sodium pyrosulfite was 0.1%.

[0107] The preparation method is as follows:

[0108] The propylene glycol and the anti-aging composition are mixed and stirred until transparent to obtain an anti-aging composition solution; then 1,3-propylene glycol and sodium metabisulfite are mixed evenly to obtain a sodium metabisulfite solution; finally, the sodium metabisulfite solution and the anti-aging composition solution are mixed evenly and subjected to high-pressure homogenization at a homogenization pressure of 15000 psi for 4 times to obtain an anti-aging composition emulsion.

[0109] Comparative Example 2

[0110] This comparative example provides an anti-aging composition emulsion, which is composed of the following components:

[0111] 1,3-Propanediol is 20%, glycerol is 43.3%, the anti-aging composition of Example 1 is 35.2%, sodium pyrosulfite is 0.1%, betaine is 0.5%, and xylitol is 0.9%.

[0112] The preparation method is as follows:

[0113] The propylene glycol and the anti-aging composition are mixed and stirred until transparent to obtain an anti-aging composition solution; then 1,3-propylene glycol, betaine, xylitol and sodium metabisulfite are stirred at 70°C until transparent to obtain a mixed solution containing a deep eutectic solvent; finally, the mixed solution and the anti-aging composition solution are evenly mixed and subjected to high-pressure homogenization at a homogenization pressure of 15000psi, and homogenization is performed 4 times to obtain an anti-aging composition emulsion.

[0114] Comparative Example 3

[0115] This comparative example provides an anti-aging composition liposome, which is composed of the following components:

[0116] 1,3-Propanediol is 20%, glycerol is 39.7%, the anti-aging composition of Example 1 is 35.2%, sodium pyrosulfite is 0.1%, and soybean lecithin is 5%.

[0117] The preparation method is as follows:

[0118] The glycerol and the anti-aging composition are mixed and stirred until transparent to obtain an anti-aging composition solution; then 1,3-propylene glycol, soy lecithin and sodium metabisulfite are stirred at 80°C for 30 minutes until transparent, and then the temperature is reduced to 50°C to obtain a mixed solution; finally, the mixed solution and the anti-aging composition solution are mixed evenly and subjected to high-pressure homogenization at a homogenization pressure of 15000 psi, and homogenization is performed 4 times to obtain anti-aging composition liposomes.

[0119] Test Example 1

[0120] The liposomes or emulsions obtained in the examples and comparative examples were subjected to dynamic light scattering detection, and their average particle sizes after 30 days were measured, and their appearances after 30 days were observed. The results are recorded in Table 1.

[0121] Table 1

[0122] Sample name 30d particle size Appearance changes Example 2 546.3 Stable, no crystallization Example 3 544.1 Stable, no crystallization Example 4 623.4 Turbid, with a few small crystals Example 5 526.5 Stable, no crystallization Example 6 728.1 Turbidity, crystallization Example 7 522.7 Stable, no crystallization Example 8 805.6 Stratification and crystallization Comparative Example 1 677.3nm Slightly turbid, no crystallization Comparative Example 2 654.3nm Slightly turbid, no crystallization Comparative Example 3 558.4nm Stable, no crystallization

[0123] Test Example 2

[0124] The liposomes or emulsions obtained in the examples and comparative examples were compared in terms of penetration ability. The specific method is as follows:

[0125] Taking oleuropein as a standard substance, 50 parts of liposomes or emulsions were weighed and then mixed with 50 parts of SIMULGELFL (1.6%) (Seppic, France) aqueous solution at 300 r / min to obtain the essence required for the test.

[0126] Skin retention rate test: The skin permeation test is carried out according to the in vitro permeation test guidelines stipulated by the OECD / OCDE European OECD Chemical Testing Guidelines.

[0127] The retention data are recorded in Table 2.

[0128] Table 2

[0129]

[0130] Comparative analysis of Table 1 and Table 2 shows that the retention of the sample solutions of Comparative Examples 1, 2 and 3 in the skin is significantly lower than that of the liposomes prepared in Example 2, and the highest retention amount can reach 2.04 μg / cm 2 .

[0131] Comparative analysis of Comparative Examples 1-3 shows that the transdermal absorption of oleuropein can be improved by introducing xylitol eutectic solvent and liposome encapsulation technology, wherein the penetration-promoting effect of the eutectic is slightly better than that of the liposome treatment. The eutectic solvent obtained by xylitol and betaine treatment is incorporated into the liposome to obtain Example 2, which has a penetration-promoting effect on oleuropein that is about 7 times that of the untreated oleuropein emulsion, 3.8 times higher than the xylitol eutectic solvent, and 4.7 times higher than the oleuropein liposome. Therefore, this solution combines the xylitol eutectic solvent with the liposome encapsulation technology to significantly increase the skin retention of oleuropein.

[0132] A penetration comparison experiment was conducted using oleuropein in the anti-aging composition as a standard. As shown in Table 2, when the content of xylitol in Example 2 is 0.9%, the deep eutectic liposome composition formed with betaine has good stability and can significantly improve the retention of oleuropein in the skin; when the content of xylitol is significantly reduced to 0.3%, the retention of oleuropein in the stratum corneum of the skin is significantly reduced; when the content of xylitol is 5%, due to excessive hydrogen bond donors, the eutectic solvent formation will reach saturation, and excessive xylitol will also affect the stability of the liposome, resulting in the transdermal amount of oleuropein in the anti-aging composition being slightly lower than that in Example 2; therefore, it can be considered that the addition of 0.9% xylitol in Example 2 is the best preparation scheme on the basis of ensuring economy, stability and efficiency.

[0133] The presence of the anti-aging composition will affect the morphology of the liposomes. When the content of the anti-aging composition is low, the morphology and integrity of the liposomes will remain in a stable state, such as in Examples 5 and 7. However, due to the low content of the anti-aging composition, the skin retention amount is also low. As the content of the anti-aging composition increases, its skin retention amount also increases. However, when it increases to a higher level, its skin retention amount will decrease instead. This is because a higher level of anti-aging composition will destroy the morphology of the liposomes, thereby affecting its stability and transdermal effect. According to research, when the content of the anti-aging composition is about 35.2%, its stability and transdermal performance are both at a better level, as described in Example 2.

[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An anti-aging composition liposome, characterized in that: It includes betaine, xylitol, short-chain fatty alcohol, phospholipid, antioxidant and anti-aging composition; The anti-aging composition includes CutiFine CLR, CutiGuard CLR, bifida yeast fermentation product lysate and oleuropein.

2. The anti-aging composition liposome according to claim 1, characterized in that: In the anti-aging composition, the mass ratio of CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein is (0.5-3):(1-8):(0.5-3):(0.01-0.1), preferably 1:5:1:0.01-0.

1.

3. The anti-aging composition liposome according to claim 1, characterized in that: The preparation method of the anti-aging composition is to uniformly mix CutiFine CLR, CutiGuard CLR, bifid yeast fermentation product lysate and oleuropein to obtain the anti-aging composition.

4. The anti-aging composition liposome according to claim 1, characterized in that: The invention comprises, by mass percentage, 0.3-5% of betaine, 0.3-5% of xylitol, 50-70% of short-chain fatty alcohol, 3-8% of phospholipid, 0.1-0.5% of antioxidant and 10-45% of anti-aging composition.

5. The anti-aging composition liposome according to any one of claims 1 to 4, characterized in that: The short-chain fatty alcohol includes at least one of ethanol, 1,2-propylene glycol, 1,3-propylene glycol, glycerol, butylene glycol, pentanediol, and hexanediol, preferably 1,3-propylene glycol and glycerol.

6. The anti-aging composition liposome according to any one of claims 1 to 4, characterized in that: The phospholipids include soybean lecithin and / or hydrogenated lecithin; Preferably, the antioxidant comprises at least one of sodium metabisulfite, sodium thiosulfate, L-cysteine ​​and glutathione.

7. A method for preparing the anti-aging composition liposome according to any one of claims 1 to 6, characterized in that: The following steps are involved: A. Evenly mix betaine, xylitol and some short-chain fatty alcohols, and then add phospholipids thereto and mix evenly to obtain a mixed solution; B. Evenly mix the remaining short-chain fatty alcohol, antioxidant and anti-aging composition, add them into the mixed solution to obtain a uniform solution, and finally homogenize the uniform solution to obtain the anti-aging composition liposome.

8. The preparation method according to claim 7, characterized in that: In step A, the mixing temperature is 50 to 80° C.; Preferably, adding to the mixed solution under stirring; Preferably, the stirring speed is 300-400 rpm and the stirring time is 5-15 min.

9. The preparation method according to claim 7, characterized in that: The homogenization pressure is 15000-25000psi; Preferably, the number of cycles of the homogenization is 1 to 5 times; Preferably, the discharge temperature after homogenization is 10-30°C.

10. A cosmetic, characterized in that: The anti-aging composition liposome comprises the anti-aging composition liposome according to any one of claims 1 to 6.

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