Portable milch goat recessive mastitis diagnostic device and diagnostic method based on visible-near infrared absorption spectrum

Abstract

The invention provides a diagnosis device and method based on a visible-near infrared absorption spectrum technology. Comprising a spectrum acquisition device and a host. By establishing a regression model of visible-near infrared absorption light transmission spectrum data and the number of staphylococcus aureus in goat milk, the correlation between the number of bacteria and the milk goat mastitis is determined, and spectrum data are collected in real time to evaluate the health state of the milk goat mastitis, so that effective diagnosis of the milk goat mastitis is realized. The method has the advantages of being easy and convenient to operate, short in consumed time, high in accuracy rate and the like, accurate detection of milch goat mastitis can be easily achieved, and powerful support is provided for milch goat health management and disease prevention and control.

Description

Technical Field

[0001] The invention belongs to the technical field of nondestructive testing, and in particular relates to a portable dairy goat latent mastitis diagnosis device and a diagnosis method based on visible-near infrared absorption spectroscopy. Background Art

[0002] As one of the major dairy goat breeding countries, my country's goat milk production accounts for about 2% of the global total. Goat milk is rich in essential amino acids and various minerals such as potassium, magnesium, iron, and zinc. Its protein and fat particles are small and evenly distributed, so goat milk is easy to digest and absorb, and has high nutritional value and low allergy. With the improvement of consumers' awareness of healthy diet, the market potential of goat milk products with high nutritional value is huge. However, in the development of the goat milk industry, the occurrence of diseases such as mastitis is undoubtedly a major challenge faced by various breeding units. Infections with bacteria such as Staphylococcus aureus, substandard breeding environment hygiene, and untimely care during the dry period can all cause mastitis. Mastitis not only leads to a decrease in milk production in dairy goats, but also changes the nutritional components in goat milk. Even its pathogens may be transmitted to consumers through goat milk, seriously threatening human health.

[0003] Scientific feeding management and standardized health monitoring have become standard practices in modern dairy goat farming, and accurate diagnosis of mastitis is an important part of dairy goat health management. Timely and accurate identification of dairy goats with mastitis is crucial for early implementation of treatment measures and reduction of economic losses. However, traditional diagnostic methods such as clinical observation, temperature detection, ultrasonic scanning, and currently commonly used milk testing methods still have the following limitations in the rapid diagnosis of mastitis: (1) Clinical observation method: It relies on personal experience, is highly subjective, and is prone to misdiagnosis.

[0004] (2) Temperature measurement: Mastitis may not cause elevated body temperature in all cases.

[0005] (3) Breast milk testing: It is necessary to collect breast milk samples, which is a complicated and time-consuming process. Summary of the invention

[0006] In view of this, it is crucial to develop a simple, fast, accurate method for diagnosing mastitis in dairy sheep that requires low operator skills.

[0007] In order to meet the above challenges, the present invention proposes a diagnostic method based on visible-near infrared absorption spectroscopy technology. By establishing a regression model of visible-near infrared absorption light transmission spectral data and the number of Staphylococcus aureus in goat milk, the correlation between the number of bacteria and goat mastitis is determined, and the spectral data is collected in real time to evaluate its health status, thereby achieving effective diagnosis of goat mastitis. This method has the characteristics of simple operation, short time consumption, high accuracy, etc., which helps to achieve accurate detection of goat mastitis and provides strong support for goat health management and disease prevention and control.

[0008] The device described in the present invention comprises a spectrum collection device and a host; The spectrum acquisition device and the host are connected via wired or wireless means; Preferably, optical fiber connection is used to improve data transmission efficiency and effectiveness.

[0009] The spectrum collection device comprises a halogen lamp, a dairy goat breast cup, and a condenser; The halogen lamp and the condenser are arranged opposite to each other on both sides of the milk goat udder cup; the milk goat udder cup is customized according to the size of the milk goat nipple.

[0010] The host includes a spectrometer and an industrial computer; The spectrometer receives data from the spectrum acquisition device; The central controller in the industrial computer performs data processing; The data processing includes a diagnostic procedure for latent mastitis in dairy sheep.

[0011] Specifically, the diagnostic procedure for latent mastitis in dairy goats includes the following steps: Acquiring data from the spectrum acquisition device; Processing to obtain transmission spectrum data; The data obtained by comparing the correlation between visible-near infrared absorption light transmission spectrum data and the number of Staphylococcus aureus in goat milk; Determine whether it is latent mastitis in dairy sheep; Output the diagnostic results.

[0012] On the basis of the above solution, a heat dissipation device is provided on one side of the halogen lamp to facilitate long-term operation.

[0013] On the basis of the above solution, a holding device including a handle is arranged on the lower side of the goat breast cup.

[0014] On the basis of the above scheme, the host comprises an industrial computer, a spectrometer and a housing; the housing of the host is connected to the bottom plate, and the spectrometer is fixed therebetween; the industrial computer is embedded in the housing of the host.

[0015] Based on the above solution, a display device is included to display the information of the diagnostic device and the diagnosis result.

[0016] In a second aspect, a portable method for diagnosing latent mastitis in dairy goats based on visible-near infrared absorption spectroscopy is provided, comprising the following steps: The number of Staphylococcus aureus was measured using a petri dish; To determine the correlation between the number of Staphylococcus aureus and mastitis; Establish a correlation model between transmission spectrum data and the number of Staphylococcus aureus units in goat milk; Using the above diagnostic device to process and obtain visible-near infrared absorption light transmission spectrum data; The data obtained by comparing the correlation between visible-near infrared absorption light transmission spectrum data and the number of Staphylococcus aureus in goat milk; Determine whether it is latent mastitis in dairy sheep.

[0017] Specifically, the method of measuring the number of Staphylococcus aureus using a culture dish comprises the following steps: Sample processing: The collected goat milk samples were diluted to facilitate colony counting on culture plates; Spread inoculation: Spread the diluted goat milk sample onto the selective culture medium; Culture observation: Place the inoculated culture dish in an incubator and culture it under a suitable environment; count the colonies when they grow to a suitable number; Bacterial count: According to the number of colonies on the culture dish and the dilution multiple, the number of units of Staphylococcus aureus in goat milk is calculated.

[0018] Specifically, the judgment criterion is a threshold value of the number of Staphylococcus aureus in goat milk.

[0019] Specifically, the correlation model specifically includes: establishing a linear model, taking the transmission spectrum data as an independent variable and the number of Staphylococcus aureus units in goat milk as a dependent variable; The number of Staphylococcus aureus units in goat milk was predicted by inputting the transmission spectrum data into the model.

[0020] Beneficial effects of the present invention: (1) The present invention proposes a method for detecting latent mastitis in dairy goats based on visible-near infrared absorption spectroscopy. The method has the advantages of being simple, non-destructive and rapid. At the same time, the developed detection device is compact and easy to carry, suitable for mobile detection environments such as pastures, and meets the needs of different scenarios.

[0021] (2) The operation process of the present invention is simple. It only requires placing the nipple of the dairy goat into a special container, and the test results can be displayed in real time on the display device. Most of the detection and data processing work is automatically completed by the central controller of the industrial computer, which significantly reduces the complexity of the operation and has low requirements on the technical level and experience of the staff. It is very suitable for promotion in practical application scenarios such as farms.

[0022] (3) The present invention uses a halogen lamp as a light source, which has the advantages of long service life, low energy consumption, low heat level and stable light emission, thereby reducing the cost of the equipment. The close integration of the light source component and the detection device ensures that the equipment can maintain efficient working performance in various environments.

[0023] (4) The visible-near infrared absorption spectrum detection technology of the present invention has good scalability. It can not only be used for the detection of mastitis in dairy sheep, but also can be migrated to other health detection fields, providing technical support and application prospects for the non-destructive detection of various diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The present invention has the following accompanying drawings: Figure 1 Overall diagram of the goat mastitis diagnostic device; Figure 2 Schematic diagram of the spectrum collection device; Figure 3 Schematic diagram of the host structure; Figure 4 Schematic diagram of the structure of the goat mastitis diagnostic device.

[0025] Reference numerals: 1: spectrum collection device 2: host 3: optical fiber 4: cooling fan 5: halogen lamp 6: halogen lamp fixing ring 7: lamp holder 8: air inlet 9: Handle 10: Dairy goat breast cup 11: Condenser 12: Industrial computer 13: Host shell 14: DC switching power supply 15: Fiber optic interface 16: Spectrometer 17: Spectrometer base 18: Spectrometer fixing slot 19: Bottom plate 20: Screw hole DETAILED DESCRIPTION

[0026] In order to make the objects, advantages and features of the present invention more obvious, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0027] like Figure 1-3 As shown, a specific embodiment of a portable dairy goat latent mastitis diagnostic device based on visible-near infrared absorption spectroscopy is provided.

[0028] like Figure 1As shown, the device mainly consists of a spectrum acquisition device 1 and a host 2, wherein the spectrum acquisition device 1 and the host 2 are connected via an optical fiber 3. Those skilled in the art will appreciate that the connection may also be made via other communication cable forms or wireless communication methods such as Bluetooth and 4G.

[0029] The maximum size of the spectrum acquisition device is 95×60×170mm, the main unit size is 115×90×45mm, and the optical fiber length is about 100mm.

[0030] like Figure 2 As shown, the spectrum acquisition device 1 mainly consists of 4: cooling fan, 5: halogen lamp, 6: halogen lamp fixing ring, 7: lamp holder, 8: air inlet, 9: handle, 10: goat udder cup, 11: condenser, etc. The cooling fan 4 is fixed to the lamp holder 7 by screws, and the halogen lamp 5 is embedded in the lamp holder 7 by the halogen lamp fixing ring 6. The condenser 11 has threads and is fixed to the goat udder cup 10 by this.

[0031] like Figure 3 As shown, the host 2 is mainly composed of an industrial computer, a spectrometer and a housing. The host housing 13 and the bottom plate 19 are connected by screws through screw holes 20, and the spectrometer 16 is fixed between the two. The industrial computer 12 is embedded in the host housing 13, and is connected to the spectrometer 16 through a USB data cable to power it and transmit data. The condenser 11 and the optical fiber interface 15 are connected through optical fibers to achieve the transmission of optical signals. The DC switching power supply 14 is connected to the cooling fan 4, the halogen lamp 5 and the industrial computer 12 through wires to achieve stable power supply. The spectrometer 16 is firmly mounted on the bottom plate 19 through the cooperation of the base 17 and the fixing groove 18. Among them, the size of the DC switching power supply 14 is 50×20×10mm, and the diameter of the screw hole 20 is 5mm.

[0032] Based on the diagnostic device of this embodiment, a method for detecting latent mastitis in dairy goats based on visible-near infrared absorption spectroscopy is proposed. Figure 1-4 Describe the usage process.

[0033] Including preliminary preparation: Method for measuring the number of Staphylococcus aureus using a culture dish (1) Sample processing: The collected goat milk samples were diluted to facilitate colony counting on the culture plate.

[0034] (2) Spread inoculation: Spread the diluted goat milk sample onto the selective culture medium.

[0035] (3) Cultivation observation: Place the inoculated culture dish in an incubator and culture it under a suitable environment. When the colonies grow to a suitable number, count the colonies.

[0036] (4) Bacterial count: Calculate the number of Staphylococcus aureus units in goat milk based on the number of colonies on the culture dish and the dilution factor.

[0037] Determine the correlation between Staphylococcus aureus counts and mastitis In a series of experiments, the method of measuring the number of Staphylococcus aureus in a petri dish was used to compare the number of Staphylococcus aureus in the milk of healthy goats and goats with latent mastitis, and the relationship between the number of colonies and the severity of mastitis was determined. Specifically, it was measured by a "threshold value". That is, when the number of Staphylococcus aureus in goat milk exceeds a certain value, it can be judged that the dairy goat has mastitis; Establish a correlation model between transmission spectrum data and the number of Staphylococcus aureus units in goat milk By collecting the transmission spectrum data and the number of Staphylococcus aureus units of a large number of goat milk samples, a precise model was constructed using chemometric methods for practical application of portable diagnostic devices. A linear model was established, that is, "transmission spectrum data" was used as the independent variable and "number of Staphylococcus aureus units in goat milk" was used as the dependent variable. The number of Staphylococcus aureus units in goat milk was predicted by inputting the transmission spectrum data into the model.

[0038] And the usage process: When in use, first turn on the DC switching power supply 14 to ensure that the device starts normally. The power supply module uses a reusable lithium battery and is equipped with a voltage stabilization and voltage regulation circuit. The module provides stable power for the cooling fan 4, the halogen lamp 5 and the industrial computer 12 to ensure that each component works normally.

[0039] At this time, the industrial computer 12 is initialized and ready. The industrial computer 12 is connected to the spectrometer 16 via a USB data cable and provides power support for it. Click the button on the display screen of the industrial computer 12 to start the cooling fan 4 and light up the halogen lamp 5. Then put the milk goat nipple into the milk goat breast cup 10, making sure that the nipple fits the inner wall of the container to ensure the accuracy of the detection. Then the device officially starts working.

[0040] The light in the container irradiates the nipple of the goat, and the condenser 11 receives the visible-near infrared transmitted light, and transmits the optical signal to the spectrometer 16 through the optical fiber 3. The analog signal is then converted into a digital signal in the spectrometer 16 and transmitted to the central controller module of the industrial computer 12 through a USB data cable.

[0041] The data processing module embedded in the central controller module performs noise reduction, filtering, transformation and classification processing on the digital signal to obtain transmission spectrum data. The transmission spectrum data is input into the correlation model between the transmission spectrum data and the unit number of Staphylococcus aureus in goat milk, so as to accurately obtain the unit number of Staphylococcus aureus in goat milk. Finally, the value is input into the correlation model between the number of Staphylococcus aureus and mastitis to determine whether the dairy goat suffers from mastitis.

[0042] The final test results will be transmitted from the data processing module of the central controller to the display screen and displayed on the screen in real time to achieve efficient diagnosis.

[0043] It should be noted that any process or method description in the embodiments may be understood as representing a module, fragment or portion of a code including one or more executable instructions for implementing steps of a specific logical function or process, and that the scope of the preferred embodiments of the present invention includes alternative implementations in which the functions may not be performed in the order shown or discussed, including performing the functions in a substantially simultaneous manner or in the reverse order according to the functions involved, which should be understood by those skilled in the art to which the embodiments of the present invention belong.

[0044] It should be noted that the logic and / or steps in the embodiments, for example, can be considered as a sequenced list of executable instructions for implementing logical functions, and can be specifically implemented in any computer-readable medium for use by an instruction execution system, device or equipment (such as a computer-based system, a system including a processor, or other system that can fetch instructions from an instruction execution system, device or equipment and execute instructions), or in combination with these instruction execution systems, devices or equipment. For the purpose of this specification, "computer-readable medium" can be any device that can contain, store, communicate, propagate or transmit a program for use by an instruction execution system, device or equipment, or in combination with these instruction execution systems, devices or equipment. More specific examples of computer-readable media (non-exhaustive list) include the following: an electrical connection with one or more wirings (electronic device), a portable computer disk box (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable and programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disk read-only memory (CDROM). In addition, the computer-readable medium may even be paper or other suitable medium on which the program is printed, since the program may be obtained electronically, for example, by optically scanning the paper or other medium and then editing, interpreting or processing in other suitable ways if necessary, and then stored in a computer memory.

[0045] It should be understood that the various parts of the present invention can be implemented by hardware, software, firmware or a combination thereof. In the above-mentioned embodiments, a plurality of steps or methods can be implemented by software or firmware stored in a memory and executed by a suitable instruction execution system. For example, if implemented by hardware, it can be implemented by any one of the following technologies known in the art or their combination: a discrete logic circuit having a logic gate circuit for implementing a logic function for a data signal, a dedicated integrated circuit having a suitable combination of logic gate circuits, a programmable gate array (PGA), a field programmable gate array (FPGA), etc.

[0046] A person skilled in the art may understand that all or part of the steps of implementing the above-mentioned embodiment method may be completed by instructing related hardware through a program, and the program may be stored in a computer-readable storage medium, which, when executed, includes one of the steps of the method embodiment or a combination thereof.

[0047] In addition, each functional module in the embodiment of the present invention may be integrated into a processing module, or each module may exist physically separately, or two or more modules may be integrated into one module. The above-mentioned integrated module may be implemented in the form of hardware or in the form of a software functional module. If the integrated module is implemented in the form of a software functional module and sold or used as an independent product, it may also be stored in a computer-readable storage medium.

[0048] The storage medium mentioned above can be a read-only memory, a magnetic disk or an optical disk, etc.

[0049] The above embodiments describe the technical solutions of the present invention in detail. Obviously, the present invention is not limited to the described embodiments. Based on the embodiments of the present invention, people familiar with the technical field can also make various changes accordingly, but any changes that are equivalent or similar to the present invention belong to the scope of protection of the present invention.

[0050] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

Claims

1. A portable diagnostic device for latent mastitis in dairy goats based on visible-near infrared absorption spectroscopy, characterized in that: Including spectrum acquisition device and host; The spectrum acquisition device and the host are connected via wired or wireless means; The spectrum collection device comprises a halogen lamp, a dairy goat breast cup, and a condenser; The halogen lamp and the condenser are arranged opposite to each other on both sides of the goat breast cup; The host includes a spectrometer and an industrial computer; The spectrometer receives data from the spectrum acquisition device; The central controller in the industrial computer performs data processing; The data processing includes a diagnostic procedure for latent mastitis in dairy sheep.

2. The diagnostic device according to claim 1, characterized in that The diagnostic procedure for latent mastitis in dairy goats comprises the following steps: Acquiring data from the spectrum acquisition device; Processing to obtain transmission spectrum data; The data obtained by comparing the correlation between visible-near infrared absorption light transmission spectrum data and the number of Staphylococcus aureus in goat milk; Determine whether it is latent mastitis in dairy sheep; Output the diagnostic results.

3. The diagnostic device according to claim 1, characterized in that A heat dissipation device is arranged on one side of the halogen lamp.

4. The diagnostic device according to claim 1, characterized in that A holding device is arranged on the lower side of the milk goat breast sleeve cup.

5. The diagnostic device according to claim 1, characterized in that The host comprises an industrial computer, a spectrometer and a shell; the shell of the host is connected to the bottom plate, and the spectrometer is fixed between the two; and the industrial computer is embedded in the shell of the host.

6. The diagnostic device according to claim 1, characterized in that A display device is included for displaying information of the diagnosis device and diagnosis results.

7. A portable method for diagnosing latent mastitis in dairy goats based on visible-near infrared absorption spectroscopy, characterized in that: The steps include: The number of Staphylococcus aureus was measured using a petri dish; To determine the correlation between the number of Staphylococcus aureus and mastitis; Establish a correlation model between transmission spectrum data and the number of Staphylococcus aureus units in goat milk; Using the diagnostic device as described in any of claims 1 to 6 to obtain visible-near infrared absorption light transmission spectrum data; The data obtained by comparing the correlation between visible-near infrared absorption light transmission spectrum data and the number of Staphylococcus aureus in goat milk; Determine whether it is latent mastitis in dairy sheep.

8. The diagnostic method according to claim 7, characterized in that: The method of measuring the number of Staphylococcus aureus using a culture dish comprises the following steps: Sample processing: The collected goat milk samples were diluted to facilitate colony counting on culture plates; Spread inoculation: Spread the diluted goat milk sample onto the selective culture medium; Culture observation: Place the inoculated culture dish in an incubator and culture it under a suitable environment; count the colonies when they grow to a suitable number; Bacterial count: According to the number of colonies on the culture dish and the dilution multiple, the number of units of Staphylococcus aureus in goat milk is calculated.

9. The diagnostic method according to claim 7, characterized in that: The judgment criterion is the threshold value of the number of Staphylococcus aureus in goat milk.

10. The diagnostic method according to claim 7, characterized in that: The correlation model specifically includes: establishing a linear model, taking the transmission spectrum data as an independent variable and the number of Staphylococcus aureus units in goat milk as a dependent variable; The number of Staphylococcus aureus units in goat milk was predicted by inputting the transmission spectrum data into the model.

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