Microbial genetic material nucleotide extraction device and use method thereof

Abstract

The invention discloses a microbial genetic material nucleotide extraction device and a use method thereof, and relates to the technical field of nucleotide extraction. A microbial genetic material nucleotide extraction device comprises a box body, a glass plate, a control panel and a placement groove plate, a plurality of notches are formed in the top of the placement groove plate, test tubes are inserted into the notches, an electric push rod in communication connection with the control panel is arranged at the top of the box body, and the telescopic end of the electric push rod is connected with a push plate. The push plate is located on the inner top wall of the box body, a plurality of magnetic bars in one-to-one correspondence with the test tubes are arranged at the bottom of the push plate, and a plurality of anti-take-out mechanisms in one-to-one correspondence with the test tubes are arranged at the bottom of the push plate. According to the invention, by arranging the anti-take-out mechanism, a solution adhered to the surface of the magnetic bar can be pushed into the test tube, so that the magnetic bar is prevented from taking an eluent out of the test tube, and the possibility of cross contamination among samples is also avoided while the waste of the solution is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleotide extraction, and in particular to a device for extracting nucleotides from microbial genetic material and a method for using the device. Background Art

[0002] The device for extracting nucleotides from microbial genetic material usually includes a series of equipment for efficiently extracting DNA, RNA or other nucleic acids. The device includes a sample processing system, a magnetic bead separation system, a temperature control system, and a washing and elution system. The magnetic beads adsorb the nucleic acids in the microorganisms through mechanical or magnetic methods and release the purified nucleic acids in the elution solution. This device can perform automated nucleic acid extraction, improve extraction efficiency and purity, and is widely used in molecular biology research, clinical testing, food safety and other fields.

[0003] The Chinese patent with patent announcement number CN220201942U discloses a microbial nucleotide extraction device, including a box, a test tube rack is installed at the bottom of the inner cavity of the box, a rubber pad is fixedly connected to the bottom of the inner cavity of the test tube rack, a plurality of identical jacks are equidistantly provided on the top of the rubber pad, test tubes are plugged into the jacks, and vibration motors are installed on both sides of the bottom of the test tube rack. The patent sets up a box, a test tube rack, a rubber pad, a jack, a test tube, a vibration motor, a servo motor, a drive disk, a pull rod, a through hole, a connecting plate, a pin seat and a magnetic rod, and starts the vibration motor to drive the solution in the test tube rack and the test tube to oscillate at a high frequency, and drives the drive disk to rotate through the servo motor, and cooperates with the pull rod and the pin seat to pull the connecting plate to move up and down at a high frequency, thereby greatly improving the efficiency of the solution oscillation and the nucleic acid falling off the surface of the magnetic beads and dissolving into the eluent, further improving the efficiency of microbial nucleotide extraction.

[0004] However, the current extraction device has the following problems: after the extraction device extracts nucleotides in the eluent, the extracted eluent will adhere to the surface of the magnetic rod and be carried out of the test tube by the magnetic rod. The carried-out solution will cause cross-contamination between samples, affecting the reliability of the experimental results. Summary of the invention

[0005] The object of the present invention is to provide a device for extracting nucleotides from microbial genetic material and a method for using the same to solve the problems pointed out in the background technology.

[0006] The technical solution of the present invention to solve the above technical problems is as follows:

[0007] A microbial genetic material nucleotide extraction device comprises a box, a glass plate arranged on one side of the box, a control panel arranged on one side of the box, and a placement slot plate arranged on the inner bottom wall of the box, a plurality of slots are provided on the top of the placement slot plate, and test tubes are inserted in the slots, an electric push rod connected to the control panel for communication is provided on the top of the box, a push plate is connected to the telescopic end of the electric push rod, the push plate is located on the inner top wall of the box, a plurality of magnetic bars corresponding to the test tubes are provided on the bottom of the push plate, and a plurality of anti-carrying-out mechanisms corresponding to the test tubes are provided on the bottom of the push plate;

[0008] The anti-smuggling mechanism includes a track plate arranged at the bottom of the push plate and located on one side of the magnetic rod, a U-shaped block slidably arranged in the track plate, a hinged rod hinged on the side of the U-shaped block close to the magnetic rod, an elastic telescopic rod hinged on the end of the hinged rod away from the U-shaped block, and a push ring arranged at the bottom of the telescopic end of the elastic telescopic rod and in contact with the outer wall of the magnetic rod, and a spring is arranged between the side of the U-shaped block away from the hinged rod and the track plate.

[0009] Furthermore, the anti-slip mechanism also includes fixed blocks symmetrically arranged on the outer walls of both sides of the elastic telescopic rod, a long rod penetrating and laterally sliding on the two fixed blocks, a semicircular connecting block connected between the two long rods, a connecting rod arranged on the semicircular connecting block and corresponding to the elastic telescopic rod, a plurality of knocking rods arranged at intervals on one side of the connecting rod and in contact with the outer wall of the elastic telescopic rod, a fixed frame arranged at the bottom of the track plate, and a plurality of semicircular rods arranged at intervals from top to bottom on the fixed frame and corresponding to the arc surface of the semicircular connecting block, the semicircular rods are located on the motion trajectory of the semicircular connecting block, and a spring 2 is arranged between the fixed block and the semicircular connecting block.

[0010] Furthermore, the two sets of springs are arranged on the outer wall of the long rod.

[0011] Furthermore, the track plates are provided in two groups and are symmetrically distributed on both sides of the magnetic rod, and the number of U-shaped blocks, hinged rods, elastic telescopic rods, and fixing frames is equal to the number of track plates.

[0012] Furthermore, the inner wall of the track plate is provided with a slide groove matching the U-shaped block, the two side walls of the U-shaped block are equipped with balls, and the inner walls on both sides of the slide groove are provided with limit grooves matching the balls on both sides of the U-shaped block.

[0013] Furthermore, a limit block matching the groove of the U-shaped block is provided on the inner wall of one end of the slide groove away from the magnetic bar, and the length of the limit block is greater than the length of the groove of the U-shaped block.

[0014] Furthermore, the above-mentioned spring 1 is provided with two groups and is respectively located on both sides of the limit block, and the two springs 1 are respectively connected between the two ends of the U-shaped block and the inner wall of the track plate.

[0015] Furthermore, an anti-pollution mechanism is provided at the bottom of the push ring;

[0016] The anti-pollution mechanism includes a vertical rod arranged at the bottom of the push ring, and a connecting ring arranged at the bottom of the vertical rod, a brush ring is clamped at the bottom of the connecting ring, the central axis of the connecting ring and the central axis of the brush ring coincide with the central axis of the push ring respectively, and the inner diameter of the connecting ring and the inner diameter of the brush ring are equal to the inner diameter of the push ring, and a flexible brush is provided on the inner side of the brush ring.

[0017] A method for using a microbial genetic material nucleotide extraction device comprises the following steps:

[0018] S1. Operate the control panel and start the electric push rod, which pushes the push plate downward, thereby driving the magnetic rod and the anti-drag-out mechanism downward, and the bottom of the anti-drag-out mechanism contacts the top of the test tube, and finally the magnetic rod passes through the anti-drag-out mechanism and drives the magnetic beads into the test tube;

[0019] S2. When the magnetic rod is gradually extended into the test tube, the magnetic rod vibrates under the action of the anti-carry-out mechanism, so that the nucleotides adsorbed on the surface of the magnetic beads fall off more quickly into the eluent in the test tube;

[0020] S3. After the elution of the magnetic beads on the magnetic rod is completed, the electric push rod drives the push plate, the magnetic rod and the anti-carry-out mechanism to move upward and reset, and the solution adhered to the magnetic rod is pushed into the test tube through the anti-carry-out mechanism;

[0021] S4. During the resetting process, the magnetic bar will further vibrate under the action of the anti-carry-out mechanism, and the eluent adhered to the surface of the magnetic bar will quickly flow back into the test tube.

[0022] Further, S11, when the push plate moves downward, the bottom of the push ring in the anti-carry-out mechanism will contact the top of the test tube, and under the interaction force between the push ring and the test tube, the push ring will drive the elastic telescopic rod to push the U-shaped block to move through the hinged rod;

[0023] S12. The movement of the U-shaped block will squeeze the spring 1, and the push ring will squeeze the elastic telescopic rod, and finally the magnetic rod will pass through the push ring and drive the magnetic beads into the test tube.

[0024] The present invention has the following beneficial effects:

[0025] (1) The microbial genetic material nucleotide extraction device of the present invention, through the setting of the anti-carry-out mechanism, makes the track plate, U-shaped block, hinged rod, elastic telescopic rod and push ring cooperate, when the telescopic end of the electric push rod drives the push plate and the magnetic rod to move upward, the spring 1 and the elastic telescopic rod are reset by their own elastic force, so that the push ring moves downward on the surface of the magnetic rod, so that the push ring pushes the solution adhered to the surface of the magnetic rod into the test tube, thereby preventing the magnetic rod from carrying the eluent out of the test tube, avoiding the waste of solution, and avoiding the possibility of cross contamination between samples.

[0026] (2) The present invention sets an anti-carry-out mechanism, and cooperates with a fixed block, a long rod, a semicircular connecting block, a connecting rod, a knocking rod, a fixed frame, a semicircular connecting block, and a magnetic rod. The resetting of the second spring will drive the semicircular connecting block, the connecting rod, and the knocking rod to reset. In this way, the knocking rod will knock the elastic telescopic rod, thereby causing the elastic telescopic rod to vibrate. The vibration of the elastic telescopic rod will be transmitted to the magnetic rod through the push ring, thereby causing the magnetic rod to vibrate, so that the nucleotides on the surface of the magnetic beads fall off into the eluent more quickly. At the same time, through the cooperation of the elastic telescopic rod, the push ring, and the magnetic rod, when the push ring pushes the solution adhered to the surface of the magnetic rod into the test tube, the elastic telescopic rod will also vibrate. The vibration of the elastic telescopic rod will be transmitted to the magnetic rod through the push ring, thereby causing the solution adhered to the surface of the magnetic rod to flow back into the test tube faster.

[0027] (3) The present invention provides an anti-pollution mechanism so that the brush ring and the magnetic rod cooperate to enable the magnetic rod to adsorb the magnetic beads suspended in the buffer solution or other solutions. The magnetic beads will pass through the brush ring, and the brush ring will clean the buffer solution or other solutions on the surface of the magnetic beads. The brush ring is clamped on the bottom of the connecting ring to facilitate the removal and replacement of the brush ring. After completing an extraction step, the brush ring can be replaced, thereby reducing the interference of impurities on the surface of the magnetic beads and other solutions, and improving the washing and elution effect of the eluent on the nucleotides on the surface of the magnetic beads. Since the nucleotides are usually combined with the magnetic beads through electrostatic effects, hydrogen bonds or other non-covalent interactions, the magnetic beads with strong adsorption force can generally better retain the nucleotides. This adsorption force is sufficient to resist the physical effects of the flexible brush and the push ring, and will not affect the nucleotides on the surface of the magnetic beads. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of the device for extracting nucleotides from microbial genetic material;

[0029] Figure 2 It is a schematic diagram of the side section structure of a device for extracting nucleotides from microbial genetic material;

[0030] Figure 3 It is a schematic diagram of the structure of the anti-carry-out mechanism;

[0031] Figure 4 A schematic diagram of the specific structure of the anti-carry-out mechanism;

[0032] Figure 5 for Figure 4 A schematic diagram of the enlarged structure at point A;

[0033] Figure 6 for Figure 4 A schematic diagram of the enlarged structure at B;

[0034] Figure 7 This is a schematic diagram of the structure of the anti-pollution mechanism.

[0035] In the figure: 1. box body; 2. glass plate; 3. control panel; 4. placement slot plate; 5. test tube; 6. electric push rod; 7. push plate; 71. magnetic rod; 8. anti-carrying out mechanism; 81. track plate; 8101. slide groove; 8102. limit groove; 8103. limit block; 82. U-shaped block; 821. groove; 83. hinged rod; 84. elastic telescopic rod; 85. push ring; 86. spring one; 87. fixed block; 88. long rod; 89. semicircular connecting block; 810. connecting rod; 811. knock rod; 812. fixed frame; 813. semicircular rod; 814. spring two; 9. anti-pollution mechanism; 91. vertical rod; 92. connecting ring; 93. brush ring. DETAILED DESCRIPTION

[0036] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0037] like Figure 1 and Figure 2As shown, an embodiment of the present invention provides a microbial genetic material nucleotide extraction device, including a box body 1, the box body 1 is hollow inside, a glass plate 2 is arranged on one side of the box body 1, the glass plate 2 is arranged on the front side of the box body 1, and the arrangement of the glass plate 2 is convenient for operators to observe the specific operation conditions in the box body 1; a control panel 3 is arranged on one side of the box body 1, and the control panel 3 is arranged on the front side of the box body 1; and a placement slot plate 4 is arranged on the inner bottom wall of the box body 1, and a plurality of slots are opened on the top of the placement slot plate 4, and test tubes 5 are inserted in the slots. The side panels and the top panel for placing the slot panel 4 cooperate as the sample room of the extraction device; the top of the box body 1 is provided with an electric push rod 6 that is connected to the control panel 3 for communication. The electric push rod 6 is fixed to the top of the box body 1 through a mounting frame. The top of the box body 1 is provided with a through hole that matches the telescopic end of the electric push rod 6. The telescopic end of the electric push rod 6 is connected with a push plate 7. The push plate 7 is located on the inner top wall of the box body 1. The telescopic end of the electric push rod 6 passes through the through hole on the top of the box body 1 and is connected to the top of the push plate 7; the bottom of the push plate 7 is provided with a plurality of magnetic rods 71 ​​that correspond to the test tubes 5 one by one. The electric push rod 6 is started by operating the control panel 3. The telescopic end of the electric push rod 6 pushes the push plate 7 to drive the magnetic rod 71 to move downward, and the magnetic rod 71 is extended into the test tube 5 below, so that the nucleotides adsorbed on the surface of the magnetic beads fall off into the eluent in the test tube 5.

[0038] like Figures 2 to 6 As shown, in order to prevent the extracted eluent from adhering to the surface of the magnetic rod 71 and being carried out of the test tube 5 by the magnetic rod 71, and to prevent the carried-out solution from causing cross-contamination between samples, a plurality of carry-out prevention mechanisms 8 corresponding to the test tubes 5 are provided at the bottom of the push plate 7;

[0039] The anti-smuggling mechanism 8 includes a track plate 81 arranged at the bottom of the push plate 7 and located on one side of the magnetic rod 71, and a U-shaped block 82 slidably arranged in the track plate 81. Specifically, the inner wall of the track plate 81 is provided with a slide groove 8101 matching the U-shaped block 82, and the two side walls of the U-shaped block 82 are installed with ball bearings (not shown in the figure), and the inner walls on both sides of the slide groove 8101 are provided with limiting grooves 8102 matching the ball bearings on both sides of the U-shaped block 82; a hinge rod 83 hinged on the side of the U-shaped block 82 close to the magnetic rod 71, an elastic telescopic rod 84 hinged on the end of the hinge rod 83 away from the U-shaped block 82, and a push ring 85 arranged at the bottom of the telescopic end of the elastic telescopic rod 84 and in contact with the outer wall of the magnetic rod 71, and a spring 86 is arranged between the side of the U-shaped block 82 away from the hinge rod 83 and the track plate 81. Among them, the hinged rod 83 and the elastic telescopic rod 84 are arranged vertically, and the elastic telescopic rod 84 specifically includes an outer tube and an inner rod axially sliding in the outer tube, the inner rod serves as a telescopic end, and a reset spring is connected between the inner rod and the bottom wall of the outer tube. The outer tube in the elastic telescopic rod 84 is hinged to the hinged rod 83, and the end of the inner rod in the elastic telescopic rod 84 away from the outer tube is connected to the push ring 85, and the push ring 85 is a circular ring structure. The push ring 85 is sleeved on the outer wall of the magnetic rod 71, and its inner wall is in contact with the outer wall of the magnetic rod 71. The push ring 85 includes an outer ring and an inner ring arranged on the inner side of the outer ring, and the inner ring is made of a flexible material, such as silicone, rubber, etc.; because nucleotides are usually combined with magnetic beads through electrostatic action, hydrogen bonds or other non-covalent interactions, magnetic beads with strong adsorption force can generally better retain nucleotides, and this adsorption force is sufficient to resist the physical action of the push ring 85 and will not affect the nucleotides on the surface of the magnetic beads.

[0040] In this embodiment, a stopper 8103 matching the groove 821 of the U-shaped block 82 is disposed on the inner wall of one end of the slide slot 8101 away from the magnetic bar 71, and the length of the stopper 8103 is greater than the length of the groove 821 of the U-shaped block 82, and the stopper 8103 is used to limit the sliding position of the U-shaped block 82. Two groups of springs 1 86 are provided and are respectively located on both sides of the stopper 8103, and the two springs 1 86 are respectively connected between the two ends of the U-shaped block 82 and the inner wall of the track plate 81.

[0041] When in use, the electric push rod 6 is started through the control panel 3. When the telescopic end of the electric push rod 6 is extended, it will push the push plate 7 to move downward. The downward movement of the push plate 7 will drive the magnetic beads to extend into the test tube 5 through the magnetic rod 71, so that the eluent inside the test tube 5 will remove the nucleotides adsorbed on the surface of the magnetic beads, thereby achieving the effect of nucleotide extraction; when the push plate 7 moves downward, the bottom of the push ring 85 will conflict with the top of the test tube 5, and under the interaction force between the push ring 85 and the test tube 5, the push ring 85 will push the U-shaped block 82 to move through the elastic telescopic rod 84 and the hinged rod 83, and the U-shaped block 82 The movement will squeeze the spring 1 86, and the push ring 85 will squeeze the elastic telescopic rod 84, thereby ensuring that the magnetic rod 71 and the magnetic beads can enter the test tube 5; when the elution is completed, the telescopic end of the electric push rod 6 drives the push plate 7 and the magnetic rod 71 to move upward, and the spring 1 86 and the elastic telescopic rod 84 are reset by their own elastic force, so that the push ring 85 moves downward on the surface of the magnetic rod 71, thereby pushing the solution adhered to the surface of the magnetic rod 71 into the test tube 5, preventing the magnetic rod 71 from taking the extracted solution out of the test tube 5, avoiding solution waste, and avoiding the possibility of cross contamination between samples.

[0042] like Figure 4 and Figure 5 As shown, the anti-smuggling mechanism 8 also includes fixed blocks 87 symmetrically arranged on the outer walls of both sides of the elastic telescopic rod 84, and the fixed blocks 87 are symmetrically fixed to the outer walls of both sides of the outer tube of the elastic telescopic rod 84; a long rod 88 that penetrates and slides laterally on the two fixed blocks 87, and a sliding hole matching the long rod 88 is opened on the fixed block 87, and a semicircular connecting block 89 connected between the two long rods 88, the semicircular connecting block 89 is located at one end of the two long rods 88 away from the magnetic rod 71, and the arc surface of the semicircular connecting block 89 faces the magnetic rod 71; a connecting rod 810 arranged on the semicircular connecting block 89 and corresponding to the elastic telescopic rod 84, and the connecting rod 810 is fixed to the semicircular connecting block 89 is on the side away from the magnetic rod 71, and a plurality of knocking rods 811 are arranged at intervals on one side of the connecting rod 810 and in contact with the outer wall of the elastic telescopic rod 84, all the knocking rods 811 are arranged at intervals from top to bottom, and the knocking rods 811 are in contact with the outer tube on the elastic telescopic rod 84; a fixing frame 812 is arranged at the bottom of the track plate 81, and a plurality of semicircular rods 813 are arranged at intervals from top to bottom on the fixing frame 812 and correspond to the arc surface of the semicircular connecting block 89, the semicircular rods 813 are located on the movement trajectory of the semicircular connecting block 89, and a spring 2 814 is arranged between the fixing block 87 and the semicircular connecting block 89; and the spring 2 814 is sleeved on the outer wall of the long rod 88.

[0043] When the push ring 85 squeezes the elastic telescopic rod 84, the inner rod of the elastic telescopic rod 84 moves upward along the outer tube, and the elastic telescopic rod 84 pushes the U-shaped block 82 to slide along the slide groove 8101 in the track plate 81 through the hinge rod 83. At the same time, the elastic telescopic rod 84 moves upward, which will drive the fixed block 87 to move. The movement of the fixed block 87 will drive the long rod 88 and the semicircular connecting block 89 to move upward. When the arc surface of the semicircular connecting block 89 contacts the arc surface of the semicircular rod 813, under the sliding cooperation between the long rod 88 and the fixed block 87, the semicircular connecting block 89 will be pushed to move away from the elastic telescopic rod 84. At this time, the semicircular connecting block 89 makes the spring 81 4 is stretched, and at the same time, the connecting rod 810 drives the knocking rod 811 away from the elastic telescopic rod 84; when the arc surface of the semicircular connecting block 89 is not in contact with the arc surface of the semicircular rod 813, the second spring 814 is reset under the action of its elastic force, and the reset of the second spring 814 will drive the semicircular connecting block 89, the long rod 88, and the connecting rod 810 to reset, and then the knocking rod 811 will knock the outer wall of the elastic telescopic rod 84, and so on, so that the elastic telescopic rod 84 vibrates, and the vibration of the elastic telescopic rod 84 is transmitted to the magnetic rod 71 through the push ring 85, so that the magnetic rod 71 vibrates, so that the nucleotides on the surface of the magnetic beads fall off faster into the eluent of the test tube 5.

[0044] When the spring 1 86 drives the U-shaped block 82 to reset and the elastic telescopic rod 84 to reset, the push ring 85 moves downward on the surface of the magnetic rod 71 and pushes the solution adhered to the surface of the magnetic rod 71 into the test tube 5. With the cooperation of the semicircular connecting block 89, the connecting rod 810, the knocking rod 811 and the semicircular rod 813, the elastic telescopic rod 84 will also vibrate. The vibration of the elastic telescopic rod 84 will be transmitted to the magnetic rod 71 through the push ring 85, thereby causing the magnetic rod 71 to vibrate, thereby accelerating the speed at which the solution adhered to the surface of the magnetic rod 71 flows back into the test tube 5.

[0045] Specifically, the track plates 81 in this embodiment are provided with two groups and are symmetrically distributed on both sides of the magnetic rod 71. The number of U-shaped blocks 82, hinged rods 83, elastic telescopic rods 84, and fixed frames 812 are equal to the number of track plates 81, which can further improve the stability of the push ring 85.

[0046] like Figure 3 and Figure 7 As shown, another embodiment of the present invention provides a microbial genetic material nucleotide extraction device, and an anti-pollution mechanism 9 is provided at the bottom of the push ring 85;

[0047] Specifically, the anti-pollution mechanism 9 includes a vertical rod 91 arranged at the bottom of the push ring 85, and the vertical rod 91 is fixed to the bottom of the outer ring of the push ring 85; and a connecting ring 92 arranged at the bottom of the vertical rod 91, and the bottom of the connecting ring 92 is clamped with a brush ring 93, and the bottom of the connecting ring 92 is provided with a slot, and the top of the brush ring 93 is provided with a strip clamped with the slot, thereby facilitating the disassembly, assembly and replacement of the brush ring 93; the central axis of the connecting ring 92 and the central axis of the brush ring 93 coincide with the central axis of the push ring 85 respectively, and the inner diameter of the connecting ring 92 and the inner diameter of the brush ring 93 are equal to the inner diameter of the push ring 85, and a flexible brush is provided on the inner side of the brush ring 93.

[0048] During use, when the magnetic bar 71 adsorbs the magnetic beads suspended in the buffer or other solution, the magnetic beads will pass through the brush ring 93, and the flexible brush on the brush ring 93 will clean the buffer or other solution on the surface of the magnetic beads, reducing the interference of impurities or other solutions on the surface of the magnetic beads, thereby improving the washing and elution effect of the eluent on the nucleotides on the surface of the magnetic beads. Since the nucleotides are usually combined with the magnetic beads through electrostatic effects, hydrogen bonds or other non-covalent interactions, magnetic beads with strong adsorption force can generally better retain the nucleotides, and this adsorption force is sufficient to resist the physical action of the flexible brush on the brush ring 93, thereby ensuring that the flexible brush only cleans the solution on the surface of the magnetic beads and does not affect the nucleotides on the surface of the magnetic beads.

[0049] Another embodiment of the present invention also provides a method for using a microbial genetic material nucleotide extraction device, comprising the following steps:

[0050] S1, operate the control panel 3 and start the electric push rod 6, the electric push rod 6 will push the push plate 7 to move downward, and then drive the magnetic rod 71 and the anti-carry-out mechanism 8 to move downward, the bottom of the anti-carry-out mechanism 8 contacts the top of the test tube 5, and finally the magnetic rod 71 passes through the anti-carry-out mechanism 8 and drives the magnetic beads to extend into the test tube 5;

[0051] S11, when the push plate 7 moves downward, the bottom of the push ring 85 in the anti-carry-out mechanism 8 will contact the top of the test tube 5, and under the interaction force between the push ring 85 and the test tube 5, the push ring 85 will drive the elastic telescopic rod 84 to push the U-shaped block 82 to move through the hinge rod 83;

[0052] S12, the movement of the U-shaped block 82 will squeeze the spring 1 86, and the push ring 85 will squeeze the elastic telescopic rod 84, and finally the magnetic bar 71 will pass through the push ring 85 and drive the magnetic beads to extend into the test tube 5;

[0053] S2, when the magnetic bar 71 gradually extends into the test tube 5, the magnetic bar 71 vibrates under the action of the anti-carry-out mechanism 8, so that the nucleotides adsorbed on the surface of the magnetic beads fall off more quickly into the eluent in the test tube 5;

[0054] When the elastic telescopic rod 84 moves upward, the fixed block 87 will be driven to move, and the movement of the fixed block 87 will drive the long rod 88 and the semicircular connecting block 89 to move upward. When the arc surface of the semicircular connecting block 89 contacts the arc surface of the semicircular rod 813, the semicircular connecting block 89 will be pushed to move in the direction away from the elastic telescopic rod 84. At this time, the spring 2 814 is stretched by the semicircular connecting block 89, and the connecting rod 810 drives the knocking rod 811 away from the elastic telescopic rod 84; when the arc surface of the semicircular connecting block 89 does not contact the arc surface of the semicircular rod 813, the spring 2 814 is reset under the action of its elastic force, and the reset of the spring 2 814 will drive the semicircular connecting block 89, the long rod 88, and the connecting rod 810 to reset, and then the outer wall of the elastic telescopic rod 84 is knocked by the knocking rod 811, and the magnetic bar 71 can vibrate in this reciprocating manner.

[0055] S3, after the elution of the magnetic beads on the magnetic rod 71 is completed, the electric push rod 6 drives the push plate 7, the magnetic rod 71 and the anti-carry-out mechanism 8 to move upward and reset, and the solution adhered to the magnetic rod 71 is pushed into the test tube 5 through the anti-carry-out mechanism 8;

[0056] S4, during the resetting process, the magnetic bar 71 will further vibrate under the action of the anti-carry-out mechanism 8, and the eluent adhered to the surface of the magnetic bar 71 will quickly flow back into the test tube 5;

[0057] S41, when the telescopic end of the electric push rod 6 drives the push plate 7 and the magnetic rod 71 to move upward, the spring 1 86 and the elastic telescopic rod 84 are reset, thereby driving the push ring 85 to move from top to bottom on the surface of the magnetic rod 71, so that the push ring 85 pushes the solution adhered to the surface of the magnetic rod 71 into the test tube 5.

[0058] S42. With the cooperation of the semicircular connecting block 89, the connecting rod 810, the knocking rod 811 and the semicircular rod 813, the elastic telescopic rod 84 will vibrate, and the vibration of the elastic telescopic rod 84 will be transmitted to the magnetic rod 71 through the push ring 85, causing the magnetic rod 71 to vibrate, thereby accelerating the speed at which the solution adhered to the surface of the magnetic rod 71 flows back into the test tube 5.

[0059] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial genetic material nucleotide extraction device, comprising a housing (1), a glass plate (2) arranged on one side of the housing (1), a control panel (3) arranged on one side of the housing (1), and a placement slot plate (4) arranged on the inner bottom wall of the housing (1), the placement slot plate (4) having a plurality of slots on the top, and test tubes (5) inserted into the slots, an electric push rod (6) communicating with the control panel (3) being arranged on the top of the housing (1), a push plate (7) being connected to the telescopic end of the electric push rod (6), the push plate (7) being located on the inner top wall of the housing (1), and a plurality of magnetic rods (71) corresponding to the test tubes (5) being arranged on the bottom of the push plate (7), characterized in that: The bottom of the push plate (7) is provided with a plurality of anti-carrying-out mechanisms (8) corresponding one to one with the test tubes (5); The anti-smuggling mechanism (8) comprises a track plate (81) arranged at the bottom of the push plate (7) and located on one side of the magnetic rod (71), a U-shaped block (82) slidably arranged in the track plate (81), a hinge rod (83) hinged on the side of the U-shaped block (82) close to the magnetic rod (71), an elastic telescopic rod (84) hinged on the end of the hinge rod (83) away from the U-shaped block (82), and a push ring (85) arranged at the bottom of the telescopic end of the elastic telescopic rod (84) and in contact with the outer wall of the magnetic rod (71), and a spring (86) is arranged between the side of the U-shaped block (82) away from the hinge rod (83) and the track plate (81).

2. The microbial genetic material nucleotide extraction device according to claim 1, characterized in that: The anti-slip mechanism (8) further comprises fixed blocks (87) symmetrically arranged on the outer walls of both sides of the elastic telescopic rod (84), long rods (88) penetrating and laterally slidably arranged on the two fixed blocks (87), a semicircular connecting block (89) connected between the two long rods (88), a connecting rod (810) arranged on the semicircular connecting block (89) and corresponding to the elastic telescopic rod (84), a plurality of knocking rods (811) arranged at intervals on one side of the connecting rod (810) and in contact with the outer wall of the elastic telescopic rod (84), a fixed frame (812) arranged at the bottom of the track plate (81), and a plurality of semicircular rods (813) arranged at intervals on the fixed frame (812) from top to bottom and corresponding to the arc surface of the semicircular connecting block (89), the semicircular rods (813) being located on the motion trajectory of the semicircular connecting block (89), and a spring 2 (814) being arranged between the fixed block (87) and the semicircular connecting block (89).

3. The microbial genetic material nucleotide extraction device according to claim 2, characterized in that: The second spring (814) is sleeved on the outer wall of the long rod (88).

4. The microbial genetic material nucleotide extraction device according to claim 2, characterized in that: The track plates (81) are provided with two groups and are symmetrically distributed on both sides of the magnetic rod (71); the number of the U-shaped blocks (82), the hinge rods (83), the elastic telescopic rods (84), and the fixing frames (812) is equal to the number of the track plates (81).

5. The microbial genetic material nucleotide extraction device according to claim 1, characterized in that: The inner wall of the track plate (81) is provided with a slide groove (8101) matching the U-shaped block (82), the two side walls of the U-shaped block (82) are equipped with ball bearings, and the inner walls on both sides of the slide groove (8101) are provided with limit grooves (8102) matching the ball bearings on both sides of the U-shaped block (82).

6. The device for extracting nucleotides from microbial genetic material according to claim 5, characterized in that: A limit block (8103) matching the groove (821) of the U-shaped block (82) is provided on the inner wall of one end of the slide groove (8101) away from the magnetic bar (71), and the length of the limit block (8103) is greater than the length of the groove (821) of the U-shaped block (82).

7. The device for extracting nucleotides from microbial genetic material according to claim 6, characterized in that: The spring one (86) is provided in two groups and is respectively located on both sides of the limit block (8103), and the two springs one (86) are respectively connected between the two ends of the U-shaped block (82) and the inner wall of the track plate (81).

8. The device for extracting nucleotides from microbial genetic material according to any one of claims 1 to 7, characterized in that: The bottom of the push ring (85) is provided with an anti-pollution mechanism (9); The anti-pollution mechanism (9) comprises a vertical rod (91) arranged at the bottom of the push ring (85), and a connecting ring (92) arranged at the bottom of the vertical rod (91); a brush ring (93) is clamped at the bottom of the connecting ring (92); the central axis of the connecting ring (92) and the central axis of the brush ring (93) respectively coincide with the central axis of the push ring (85); the inner diameter of the connecting ring (92) and the inner diameter of the brush ring (93) are equal to the inner diameter of the push ring (85); and a flexible brush is provided on the inner side of the brush ring (93).

9. A method for using the microbial genetic material nucleotide extraction device according to claim 1, characterized in that: The following steps are involved: S1, operate the control panel (3) and start the electric push rod (6), the electric push rod (6) will push the push plate (7) to move downward, thereby driving the magnetic rod (71) and the anti-carry-out mechanism (8) to move downward, the bottom of the anti-carry-out mechanism (8) contacts the top of the test tube (5), and finally the magnetic rod (71) passes through the anti-carry-out mechanism (8) and drives the magnetic beads to extend into the test tube (5); S2, when the magnetic rod (71) gradually extends into the test tube (5), the magnetic rod (71) vibrates under the action of the anti-carry-out mechanism (8), so that the nucleotides adsorbed on the surface of the magnetic beads fall off more quickly into the eluent in the test tube (5); S3, after the elution of the magnetic beads on the magnetic rod (71) is completed, the electric push rod (6) drives the push plate (7), the magnetic rod (71) and the anti-carry-out mechanism (8) to move upward and reset, and the solution adhered to the magnetic rod (71) is pushed into the test tube (5) through the anti-carry-out mechanism (8); S4. During the resetting process, the magnetic bar (71) will further vibrate under the action of the anti-carry-out mechanism (8), and the eluent adhered to the surface of the magnetic bar (71) will quickly flow back into the test tube (5).

10. The method for using the microbial genetic material nucleotide extraction device according to claim 9, characterized in that: S11. When the push plate (7) moves downward, the bottom of the push ring (85) in the anti-carry-out mechanism (8) contacts the top of the test tube (5). Under the interaction force between the push ring (85) and the test tube (5), the push ring (85) drives the elastic telescopic rod (84) to push the U-shaped block (82) to move through the hinge rod (83); S12. The movement of the U-shaped block (82) will squeeze the spring 1 (86), and at the same time the push ring (85) will squeeze the elastic telescopic rod (84), eventually causing the magnetic rod (71) to pass through the push ring (85) and drive the magnetic beads to extend into the test tube (5).

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