Long-acting foam antibacterial disinfectant and preparation method thereof

Abstract

The invention relates to the technical field of antibacterial disinfectants, and provides a long-acting foam antibacterial disinfectant and a preparation method thereof.The method comprises the steps that gelatin is added into hot water to be fully stirred and dissolved, a gelatin solution with the mass fraction being 4-8% is formed, then 1-3 drops of glutaraldehyde solution are added into the gelatin solution, eucalyptus oil is immediately added after the gelatin solution is stirred to be uniform, and the mixture is stirred to be uniform; after uniformly stirring, performing vacuum drying for 0.5 to 1h to obtain a material A; adding 1-3 drops of glutaraldehyde solution into the material A, standing for 1.5-2 hours at the temperature of 20-25 DEG C, washing with water, and carrying out vacuum drying for 0.5-1 hour at the temperature of 80-100 DEG C to obtain a material B; fully grinding the material B to form antibacterial particles smaller than 50 microns; the long-acting foam antibacterial disinfectant is obtained by adding the antibacterial particles, the biguanide disinfectant, the antibacterial peptide and the surfactant into the propylene glycol solvent and uniformly mixing. According to the technical scheme, the problem of energy consumption waste of the foam antibacterial disinfectant in the prior art is solved.

Description

Technical Field

[0001] The invention relates to the technical field of antibacterial disinfectants, and in particular to a long-acting foam antibacterial disinfectant and a preparation method thereof. Background Art

[0002] Disinfection of farms (pig farms, chicken farms, etc.) is an important measure to protect animal health and prevent the spread of diseases. The main purpose of farm disinfection is to kill or eliminate pathogenic microorganisms in the environment, including bacteria, viruses, fungi, etc., to reduce the risk of animal infection and improve the biosafety level of farms. Through disinfection, the transmission pathway of the disease can be cut off, the morbidity and mortality of the disease can be reduced, and the breeding efficiency can be improved.

[0003] When disinfecting farms, antibacterial disinfectants are needed. Traditional disinfectants are mostly in liquid form. Although they have certain bactericidal effects, they have many limitations in practical applications. When spraying, factors such as surface tension and gravity can easily lead to uneven coverage, resulting in disinfection blind spots, which affect the overall disinfection effect. Based on this, foam antibacterial disinfectants came into being. Through their unique foam form, they can more effectively cover the disinfected surface and improve the disinfection effect. At the same time, the active ingredients in the foam disinfectant can stay on the surface of the object for a longer time to form lasting protection. In addition, the foam disinfectant can also be adjusted through the formula to reduce irritation to the farm environment and improve the safety of use.

[0004] Most of the existing foam antibacterial disinfectants are composite antibacterial disinfectants, which are mixtures of different types of antibacterial disinfectants to enable them to exert their synergistic capabilities and thus enhance their bactericidal effects. They mainly rely on strict control of bactericidal ingredients and proportions, and the physical form of foam covering further enhances the antibacterial effect.

[0005] However, this method also has certain disadvantages, namely: when the same amount of antibacterial disinfectant acts on the surface of objects inside the farm, when in the form of a spray, the spray can completely cover the surface of the object and contact the object, thereby achieving the purpose of a large amount of antibacterial disinfectant taking effect. When it acts on the surface of the object in the form of foam, only the innermost layer of foam is in contact with the surface of the object, and the outer layer of foam needs to wait for the inner layer of foam to complete its effect before it can be immersed in the surface of the object. During the waiting process, the outer layer of foam will be in contact with the air for a long time, which can easily affect its antibacterial and disinfecting performance. Therefore, when the outer layer of foam acts on the surface of the object, it can no longer exert its optimal performance or even lose its antibacterial and disinfecting effect, which will reduce the antibacterial and disinfecting effect of the antibacterial disinfectant, making it unable to fully exert its effect, resulting in energy waste of the antibacterial disinfectant. Summary of the invention

[0006] The present invention provides a long-acting foam antibacterial disinfectant and a preparation method thereof, which solves the following problems in the related art:

[0007] How to prevent energy waste from foam antibacterial disinfectants?

[0008] The technical solution of the present invention is as follows:

[0009] In a first aspect, the present invention discloses a long-acting foam antibacterial disinfectant, comprising the following raw materials in parts by weight: 55-75 parts of biguanide disinfectant, 20-30 parts of antimicrobial peptide, 10-15 parts of gelatin, 15-25 parts of eucalyptus oil, 16-18 parts of surfactant, and 120-150 parts of propylene glycol solvent;

[0010] Preferably, the biguanide disinfectant is 60-70 parts, the antimicrobial peptide is 23-28 parts, the gelatin is 11-14 parts, the eucalyptus oil is 18-23 parts, the surfactant is 16-18 parts, and the propylene glycol solvent is 130-140 parts;

[0011] More preferably, the biguanide disinfectant is 65 parts, the antimicrobial peptide is 25 parts, the gelatin is 13 parts, the eucalyptus oil is 20 parts, the surfactant is 17 parts, and the propylene glycol solvent is 135 parts.

[0012] In a further embodiment of the present invention: the biguanide disinfectant is one or more of chlorhexidine, polyhexamethylene biguanide hydrochloride or polyhexamethylene biguanide;

[0013] Preferably, the biguanide disinfectant is a mixture of chlorhexidine and polyhexamethylene biguanide hydrochloride, the mass ratio of the two is 10:(3-4), more preferably, the mass ratio of the two is 10:3.5.

[0014] In a further embodiment of the present invention: the surfactant is a quaternary ammonium salt;

[0015] Preferably, the quaternary ammonium salt is one or more of arsenicum chloride, benzalkonium chloride or benzethonium chloride;

[0016] More preferably, the quaternary ammonium salt is arsenic ammonium chloride.

[0017] In a further embodiment of the present invention, the surfactant further comprises one or both of alkylbenzene sulfonate and lauric acid, preferably lauric acid.

[0018] In a further embodiment of the present invention: the mass fraction of the propylene glycol solvent is 10-20%;

[0019] Preferably, the mass fraction of propylene glycol is 12-18%, more preferably 15%.

[0020] In a second aspect, the present invention further discloses a method for preparing the long-acting foam antibacterial disinfectant as described above, comprising the following steps:

[0021] S1: Add gelatin to hot water at 50-60°C and stir thoroughly to dissolve to form a gelatin solution with a mass fraction of 4-8%, then add 1-3 drops of glutaraldehyde solution to the gelatin solution, stir for 1.5-2h and immediately add eucalyptus oil, stir evenly and transfer to an environment of 80-100°C and vacuum dry for 0.5-1h to obtain material A;

[0022] Preferably, the hot water temperature is 55°C, the mass fraction of the gelatin solution is 6%, the stirring time is 2h, the vacuum drying temperature is 90°C, and the vacuum drying time is 0.8h;

[0023] Preferably, the mass fraction of glutaraldehyde is 20-30%, preferably 25%, and the ratio of the amount added to gelatin is (0.02-0.05) mL:1 g, preferably 0.03 mL:1 g.

[0024] S2: Add 1-3 drops of glutaraldehyde solution to material A, let stand at 20-25°C for 1.5-2h, wash with water, and then place under vacuum drying at 80-100°C for 0.5-1h to obtain material B;

[0025] Preferably, the standing temperature is 20°C, the standing time is 2h, the vacuum drying temperature is 90°C, and the vacuum drying time is 0.8h;

[0026] Preferably, the mass fraction of glutaraldehyde is 20-30%, preferably 25%, and the ratio of the amount added to gelatin is (0.02-0.05) mL:1 g, preferably 0.03 mL:1 g.

[0027] S3: Grind material B fully to form antibacterial particles smaller than 50 μm;

[0028] Preferably, material B is ground by ultrasonic grinding or ball milling.

[0029] S4: adding the antibacterial particles, biguanide disinfectant, antibacterial peptide and surfactant into the propylene glycol solvent and mixing them evenly to obtain the long-acting foam antibacterial disinfectant;

[0030] Preferably, the method of adding the antibacterial microparticles, biguanide disinfectants and antimicrobial peptides into the propylene glycol solvent and mixing them evenly is: injecting the propylene glycol solvent into a mixer, then adding the antibacterial microparticles, biguanide disinfectants and antimicrobial peptides, and stirring at 500-600 rpm for 2-3 hours.

[0031] The working principle and beneficial effects of the present invention are:

[0032] (1) The present invention selects a plurality of antibacterial agents for compounding to obtain an antibacterial disinfectant with better antibacterial effect, wherein: the biguanide disinfectant has a positive charge on its surface, can interact with negatively charged bacteria and fungi, and attract each other, and can inhibit the synthesis of liposomes in the membrane after entering the cell membrane of bacteria and fungi, thereby causing apoptosis of the bacteria; the antimicrobial peptide can form a transmembrane channel on the biological cell membrane, inhibit the metabolism of functional proteins, DNA, RNA synthesis, etc., and play an antibacterial role. At the same time, the transmembrane channel is conducive to the rapid entry of other bactericides into the body, thereby achieving the function of rapid sterilization; eucalyptus oil is also a biological disinfectant, and the active ingredients inside it can penetrate the cell membrane of bacteria or fungi, destroy their integrity, cause the leakage of cell contents, make bacteria or fungi lose their activity, and cooperate with biguanide disinfectants and antimicrobial peptides to kill bacteria; quaternary ammonium salt is selected as a surfactant, and quaternary ammonium salt has a strong bactericidal function, which can further enhance the bactericidal performance of the antibacterial disinfectant of the present invention;

[0033] (2) The antimicrobial peptides and eucalyptus oil in the present invention are both biological antimicrobial agents, which are natural ingredients extracted from organisms. They are generally less toxic and have less impact on the farm environment, making them healthier and more environmentally friendly.

[0034] (3) When preparing the antibacterial disinfectant of the present invention, eucalyptus oil is first combined with gelatin to form antibacterial microparticles, wherein the eucalyptus oil, as an antibacterial substance, also plays the role of an oil phase solvent in the process of forming the antibacterial microparticles; the antibacterial microparticles are then mixed with biguanide disinfectants and antimicrobial peptides, and during the mixing process, part of the biguanide disinfectants and antimicrobial peptides will enter the interior of the antibacterial microparticles; when the antibacterial microparticles are covered on the surface of an object in the form of foam, the biguanide disinfectants, antimicrobial peptides and eucalyptus oil inside the antibacterial microparticles in the outer layer of foam are protected by gelatin to avoid direct contact with the air, thereby preventing volatilization on the one hand and preventing the gas or impurities in the air of the farm from affecting its performance on the other hand, thereby ensuring that the outer layer of foam still maintains its performance when it acts on the surface of the object;

[0035] (4) In the antibacterial disinfectant of the present invention, when the antibacterial particles come into contact with the bacterial surface, under the action of other antibacterial substances (biguanide disinfectants and antimicrobial peptides), the cell membrane of the bacteria or fungi is destroyed, the antibacterial particles enter the interior of the organism, and the antibacterial components in the antibacterial particles are slowly released, which is beneficial to improve the long-term effectiveness of the antibacterial disinfectant and enhance the bactericidal and disinfecting ability during farm cleaning. DETAILED DESCRIPTION

[0036] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0038] Example 1

[0039] Step 1: Pour 250 mL of water into a stirring tank, heat to 50° C. and keep constant temperature, add 10 g of gelatin, stir at 500 rpm for 1 hour to form a gelatin solution with a mass fraction of 4%, then drop 200 uL of 20% glutaraldehyde solution into the gelatin solution, stir at 500 rpm for 2 hours, then add 15 g of eucalyptus oil, stir at 500 rpm for 0.5 hour until uniform, then transfer to a beaker, place the beaker in a vacuum drying oven at 80° C. and vacuum dry for 1 hour to obtain material A;

[0040] Step 2: Add 200uL of 20% glutaraldehyde solution to material A, let it stand at room temperature of 20°C for 2h, wash with water, and place in a vacuum drying oven at 80°C for 1h to obtain material B;

[0041] Step 3: Add material B into a ball mill and grind it into antibacterial particles smaller than 50 μm;

[0042] Step 4: Inject 120g of 10% propylene glycol solvent into the mixer, then add the above antibacterial particles, 30g chlorhexidine, 25g polyhexamethylene biguanide hydrochloride, 20g antibacterial peptide, 10g arsenic ammonium chloride, and 6g lauric acid, and stir at 500rpm for 3h to obtain a foam antibacterial disinfectant.

[0043] Example 2

[0044] Step 1: Pour 183 mL of water into a stirring tank, heat to 55°C and keep constant temperature, add 11 g of gelatin, stir at 500 rpm for 1 hour to form a gelatin solution with a mass fraction of about 6%, then drop 330 uL of 25% glutaraldehyde solution into the gelatin solution, stir at 500 rpm for 2 hours, then add 18 g of eucalyptus oil, stir at 500 rpm for 0.5 hour until uniform, then transfer to a beaker, place the beaker in a vacuum drying oven at 90°C for 0.8 hour, and obtain material A;

[0045] Step 2: Add 330uL of 25% glutaraldehyde solution to material A, let stand at room temperature of 20°C for 2h, wash with water, and place in a vacuum drying oven at 90°C for 0.8h to obtain material B;

[0046] Step 3: Add material B into a ball mill and grind it into antibacterial particles smaller than 50 μm;

[0047] Step 4: Finally, inject 130g of 15% propylene glycol solvent into the mixer, then add the above antibacterial particles, 40g chlorhexidine, 20g polyhexamethylene biguanide hydrochloride, 23g antibacterial peptide, 10g arsenic ammonium chloride, and 6g lauric acid, stir at 550rpm for 2.5h, and obtain the foam antibacterial disinfectant after the end.

[0048] Example 3

[0049] Step 1: Pour 217 mL of water into a stirring tank, heat to 55°C and keep constant temperature, add 13 g of gelatin, stir at 500 rpm for 1 hour to form a gelatin solution with a mass fraction of about 6%, then drop 390 uL of 25% glutaraldehyde solution into the gelatin solution, stir at 500 rpm for 2 hours, then add 20 g of eucalyptus oil, stir at 500 rpm for 0.5 hour until uniform, then transfer to a beaker, place the beaker in a vacuum drying oven at 90°C for 0.8 hour, and obtain material A;

[0050] Step 2: Add 390uL of 25% glutaraldehyde solution to material A, let stand at room temperature of 20°C for 2h, wash with water, and place in a vacuum drying oven at 90°C for 0.8h to obtain material B;

[0051] Step 3: Add material B into a ball mill and grind it into antibacterial particles smaller than 50 μm;

[0052] Step 4: Finally, inject 135g of 15% propylene glycol solvent into the mixer, then add the above antibacterial particles, 40g chlorhexidine, 25g polyhexamethylene biguanide hydrochloride, 25g antibacterial peptide, 11g arsenic ammonium chloride, and 6g lauric acid, and stir at 550rpm for 2.5h to obtain a foam antibacterial disinfectant.

[0053] Example 4

[0054] Step 1: Pour 233 mL of water into a stirring tank, heat to 55°C and keep constant temperature, add 14 g of gelatin, stir at 500 rpm for 1 hour to form a gelatin solution with a mass fraction of about 6%, then drop 420 uL of 25% glutaraldehyde solution into the gelatin solution, stir at 500 rpm for 2 hours, then add 23 g of eucalyptus oil, stir at 500 rpm for 0.5 hour until uniform, then transfer to a beaker, place the beaker in a vacuum drying oven at 90°C for 0.8 hour, and obtain material A;

[0055] Step 2: Add 420uL of 25% glutaraldehyde solution to material A, let stand at room temperature of 20°C for 2h, wash with water, and place in a vacuum drying oven at 90°C for 0.8h to obtain material B;

[0056] Step 3: Add material B into a ball mill and grind it into antibacterial particles smaller than 50 μm;

[0057] Step 4: Finally, inject 140g of 15% propylene glycol solvent into the mixer, then add the above antibacterial particles, 45g chlorhexidine, 25g polyhexamethylene biguanide hydrochloride, 25g antibacterial peptide, 12g arsenic ammonium chloride, and 6g lauric acid, and stir at 550rpm for 2.5h to obtain a foam antibacterial disinfectant.

[0058] Example 5

[0059] Step 1: Pour 187.5 mL of water into a stirring tank, heat to 60°C and keep constant temperature, add 15 g of gelatin, stir at 500 rpm for 1 hour to form a gelatin solution with a mass fraction of 8%, then drop 750 uL of a 30% glutaraldehyde solution into the gelatin solution, stir at 500 rpm for 2 hours, then add 25 g of eucalyptus oil, stir at 500 rpm for 0.5 hour until uniform, then transfer to a beaker, place the beaker in a vacuum drying oven at 100°C for 0.5 hour, and obtain material A;

[0060] Step 2: Add 750uL of 30% glutaraldehyde solution to material A, let it stand at room temperature of 20°C for 2h, wash with water, and then place it in a vacuum drying oven at 100°C for 0.5h to obtain material B;

[0061] Step 3: Add material B into a ball mill and grind it into antibacterial particles smaller than 50 μm;

[0062] Step 4: Finally, inject 150g of 20% propylene glycol solvent into the mixer, then add the above antibacterial particles, 45g chlorhexidine, 30g polyhexamethylene biguanide hydrochloride, 25g antibacterial peptide, 12g arsenic ammonium chloride, and 6g lauric acid, and stir at 600rpm for 2h to obtain a foam antibacterial disinfectant.

[0063] Comparative Example 1

[0064] Compared with Example 3, the only difference is that the eucalyptus oil in step 1 is replaced by glycerol, and the other steps are exactly the same, and finally a foam antibacterial disinfectant is prepared.

[0065] Comparative Example 2

[0066] Inject 135g of 15% propylene glycol solvent into the mixer, then add 15g gelatin, 25g eucalyptus oil, 40g chlorhexidine, 25g polyhexamethylene biguanide hydrochloride, 25g antimicrobial peptide, 11g arsenic ammonium chloride, and 6g lauric acid, stir at 550rpm for 2.5h, and obtain the foam antibacterial disinfectant.

[0067] The foam antibacterial disinfectants of Examples 1-5 and Comparative Examples 1-2 were tested for bactericidal performance. The test method was based on the Technical Specifications for Identification of Veterinary Disinfectants (1992) Agricultural (Animal Medicine) No. 101, wherein the selected flora included two types of bacteria and fungi. The sampling method of the antibacterial disinfectant was as follows:

[0068] Spray the antibacterial disinfectant (dilution ratio is 1:200) on the surface of the clean glass plate through a spray gun to form a foam layer with a thickness of about 5 cm (to prevent the foam from overflowing, a fence is set around the glass plate to block it, and the height of the fence is 5 cm). After every 10 minutes, 20 minutes, 30 minutes, 40 minutes, 50 minutes, and 60 minutes, take the outermost layer of foam with a thickness of no more than 1 cm as the test sample of the antibacterial disinfectant. The specific method of sterilization is as follows:

[0069] First, prepare bacterial culture liquid, take out Staphylococcus aureus and Escherichia coli stored at -40°C, streak on soybean casein agar medium for activation, and invert and culture in a constant temperature incubator at 37°C for 24-48h; after a single colony grows on the plate, pick a single colony and inoculate it in 10mL LB medium for shake flask culture, place it in a shaker at 37°C and 120rpm for reaction for 24h, dilute the culture liquid cultured overnight in a gradient manner, take 100uL of Escherichia coli and Staphylococcus aureus culture liquid at appropriate dilution concentrations, spread on the plate, and place it in a constant temperature incubator at 37°C for inverted culture for 24h;

[0070] The prepared bacterial solution was counted for viable bacteria and then diluted with LB liquid medium to a concentration of 10 6 -10 7CFU / mL of test bacterial liquid; collect the test sample as an antibacterial disinfectant into a glass box, add 0.1g of defoaming agent (silicone oil) and stir evenly to obtain a disinfectant sample, draw 0.5mL of the test bacterial liquid into 4.5mL of the disinfectant sample, and place it in a 20℃ water bath for 5min; then immediately draw 0.5mL of the above-mentioned bacterial and drug mixture, add it to 4.5mL of neutralizing solution (now prepared with 1% sodium thiosulfate, 3% Tween 80 aqueous solution) and mix well; after neutralization for 10min, take 100uL of the bacterial liquid to coat the plate respectively, place it in a constant temperature incubator at 37℃ and invert it for overnight culture, finally count the live bacteria and calculate the bactericidal rate.

[0071] The test results are listed in Table 1. Table 1 is as follows:

[0072] Table 1

[0073]

[0074] By analyzing the data in Table 1, it can be found that the foam antibacterial disinfectants prepared in Examples 1-5 have a stronger bactericidal rate of the outermost layer of foam ear than those in Comparative Examples 1-2 as time goes by, which shows that when the foam is exposed to the air for a long time, the foam antibacterial disinfectant of the present invention still has a stronger bactericidal performance, which can prevent the foam antibacterial disinfectant from wasting energy.

[0075] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A long-acting foam antibacterial disinfectant, characterized in that: The invention comprises the following raw materials in parts by weight: 55-75 parts of biguanide disinfectant, 20-30 parts of antimicrobial peptide, 10-15 parts of gelatin, 15-25 parts of eucalyptus oil, 16-18 parts of surfactant and 120-150 parts of propylene glycol solvent.

2. The long-acting foam antibacterial disinfectant according to claim 1, characterized in that: The biguanide disinfectant is one or more of chlorhexidine, polyhexamethylene biguanide hydrochloride or polyhexamethylene biguanide.

3. The long-acting foam antibacterial disinfectant according to claim 1, characterized in that: The surfactant is a quaternary ammonium salt.

4. The long-acting foam antibacterial disinfectant according to claim 3, characterized in that: The quaternary ammonium salt is one or more of arsenic chloride, benzalkonium chloride or benzethonium chloride.

5. The long-acting foam antibacterial disinfectant according to claim 3, characterized in that: The surfactant further comprises one or both of alkylbenzene sulfonate and lauric acid.

6. The long-acting foam antibacterial disinfectant according to claim 1, characterized in that: The mass fraction of the propylene glycol solvent is 10-20%.

7. A method for preparing the long-acting foam antibacterial disinfectant according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1: Add gelatin to hot water at 50-60°C and stir thoroughly to dissolve to form a gelatin solution with a mass fraction of 4-8%, then add 1-3 drops of glutaraldehyde solution to the gelatin solution, stir for 1.5-2h and immediately add eucalyptus oil, stir evenly and transfer to an environment of 80-100°C and vacuum dry for 0.5-1h to obtain material A; S2: Add 1-3 drops of glutaraldehyde solution to material A, let stand at 20-25°C for 1.5-2h, wash with water, and then place under vacuum drying at 80-100°C for 0.5-1h to obtain material B; S3: Grind material B fully to form antibacterial particles smaller than 50 μm; S4: adding the antibacterial microparticles, biguanide disinfectants, antibacterial peptides and surfactants into the propylene glycol solvent and mixing them evenly to obtain the long-acting foam antibacterial disinfectant.

8. The method for preparing the long-acting foam antibacterial disinfectant according to claim 7, characterized in that: In steps S1-S2, the mass fraction of glutaraldehyde is 20-30%, and the ratio of the amount of glutaraldehyde added twice to the amount of gelatin is (0.02-0.05) mL:1 g.

9. The method for preparing the long-acting foam antibacterial disinfectant according to claim 1, characterized in that: In step S3, material B is ground by ultrasonic grinding or ball milling.

10. The method for preparing the long-acting foam antibacterial disinfectant according to claim 1, characterized in that: In step S4, the antibacterial microparticles, biguanide disinfectants and antimicrobial peptides are added to the propylene glycol solvent and mixed uniformly by injecting the propylene glycol solvent into a mixer, then adding the antibacterial microparticles, biguanide disinfectants and antimicrobial peptides, and stirring at 500-600 rpm for 2-3 hours.

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