Stem cell preparation system

Abstract

The invention discloses a stem cell preparation system, and particularly relates to the field of stem cell preparation, the stem cell preparation system comprises a cell extraction and cleaning module, a digestion module, a digestion termination module, a primary culture module, a subculture module and an activity detection module; the cell extracting and cleaning module is used for extracting adipose tissue cells and immersing the adipose tissue cells into a sodium chloride injection for cleaning and disinfection; the digestion module is used for cutting the adipose tissue cells into pieces, and then adding digestive enzymes into the cut adipose tissue cells to digest the adipose tissue cells; and the digestion termination module is used for adding normal saline into the digested adipose tissue cells to terminate digestion of the adipose tissue cells. According to the method, normal digestion of adipose tissue cells can be guaranteed, and the situation that the cell adherence capability and proliferation activity are poor due to the fact that digestion time is too long and enzyme has certain damage and aging to the cells is avoided.

Description

Technical Field

[0001] The present invention relates to the field of stem cell preparation, and more specifically, to a stem cell preparation system. Background Art

[0002] As human beings continue to explore the field of life sciences, cell research and application have become one of the hot topics of scientific circles and the global biomedical industry, especially the research on stem cells. Stem cells are a precious biological genetic resource that can not only treat various intractable diseases, but also be used for anti-aging health care. Their unique tissue repair ability and immune regulation function have won the favor and focus of scientists.

[0003] In regenerative medicine, adult stem cells are the most promising cell type for cell-based therapies. Human adipose tissue has been introduced as a new source of pluripotent stem cells. These so-called adipose-derived stem cells (ADSCs) are considered ideal for regenerative therapy applications. Their main advantage over mesenchymal stem cells derived from other sources, such as bone marrow, is that they can be easily and reproducibly obtained using minimally invasive techniques and with low morbidity.

[0004] The main method for preparing stem cells is enzyme digestion. The time of enzyme digestion is not easy to control. If the digestion time is not enough, it is impossible to obtain a sufficient number of cells. If the digestion time is too long, the enzyme will cause certain damage and aging to the cells, resulting in poor cell adhesion and proliferation activity.

[0005] In order to solve the above problems, a technical solution is now provided. Summary of the invention

[0006] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present invention provide a method that can ensure the normal digestion of adipose tissue cells, avoid the digestion time being too long, and avoid the enzyme causing certain damage and aging of the cells, resulting in poor cell adhesion ability and proliferation activity, so as to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above object, the present invention provides the following technical solution: comprising: a cell extraction and cleaning module, a digestion module, a digestion termination module, a primary culture module, a subculture module and an activity detection module;

[0008] The cell extraction and cleaning module is used to extract adipose tissue cells and immerse the adipose tissue cells in sodium chloride injection for cleaning and disinfection;

[0009] The digestion module is used to cut the fat tissue cells into pieces, and then add digestive enzymes into the cut fat tissue cells to digest the fat tissue cells;

[0010] The digestion termination module is used for adding physiological saline into the digesting adipose tissue cells to terminate the digestion of the adipose tissue cells;

[0011] The primary culture module is used to add the digested adipose tissue cells into serum-free complete culture medium, place them in an incubator for culture, and obtain a suspension;

[0012] The subculture module is used to centrifuge the suspension and then perform subculture to obtain stem cells;

[0013] The activity detection module is used to detect the activity of stem cells.

[0014] In a preferred embodiment, the operation process of the cell extraction and cleaning module includes the following:

[0015] Extract 100ml of adipose tissue cells, the concentration of sodium chloride injection is 0.9%, the sodium chloride injection is 120-150ml, immerse 100ml of adipose tissue cells in 120-150ml of 0.9% sodium chloride injection for cleaning and disinfection, the cleaning and disinfection time is set to 10-15min, and the adipose tissue cells are taken out after cleaning and disinfection are completed.

[0016] In a preferred embodiment, the operation process of the digestion module includes the following:

[0017] The fat tissue cells are cut into small pieces to obtain fat tissue blocks, and the particle size of the fat tissue blocks is 2 to 2.5 mm. 3 The digestive enzyme is pancreatic lipase, and pancreatic lipase is added to the adipose tissue block to digest the adipose tissue cells.

[0018] In a preferred embodiment, the process of terminating the operation of the digestion module includes the following:

[0019] During the digestion process, the adipose tissue cells are placed under an electron microscope, which is connected to a computer. The electron microscope is controlled by the computer to record the adipose tissue cells during the digestion process.

[0020] Compare the recorded images with the images of the adipose tissue cell digestion process stored in the computer to obtain the difference rate;

[0021] The difference change rate is obtained according to the obtained difference rate.

[0022] In a preferred embodiment, the process of obtaining the difference rate includes the following contents:

[0023] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t1;

[0024] Mark the difference ratio of the adipose tissue cell digestion process photos as Xi , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0025] X i The difference rate is marked as M1;

[0026] The calculation formula is: In the formula, It is the average value of the difference ratio of the photos of adipose tissue cell digestion process.

[0027] In a preferred embodiment, the process of obtaining the difference rate further includes the following contents:

[0028] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t2;

[0029] The difference ratio of the adipose tissue cell digestion process photos is marked as Y i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0030] Y i The difference rate is marked as M2;

[0031] The calculation formula is: In the formula, is the average value of the difference ratio of the photos of the adipose tissue cell digestion process;

[0032] Among them, M2<M1, a difference rate threshold is set, and when M1 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is less than the difference rate threshold, the digestion of the adipose tissue cells continues.

[0033] In a preferred embodiment, the process of obtaining the difference change rate includes the following:

[0034] Label the difference change rate as S;

[0035] in,

[0036] Set the difference change rate threshold, and perform early warning processing when S is greater than or equal to the difference change rate threshold.

[0037] In a preferred embodiment, the operation process of the primary culture module includes the following:

[0038] The CO2 concentration in the incubator was set to 4-6%, and the temperature in the incubator was set to 36-38°C.

[0039] In a preferred embodiment, the operation process of the subculture module includes the following:

[0040] When the cell confluence reaches 80-85%, centrifugation is performed to obtain stem cells.

[0041] Technical effects and advantages of a stem cell preparation system of the present invention:

[0042] 1. The present invention records the difference ratio of photos of the digestion process of adipose tissue cells taken at a certain time, then calculates the difference rate, sets the difference rate threshold, and terminates the digestion of adipose tissue cells when the difference rate is between the difference rate threshold. When the difference rate is less than the difference rate threshold, the digestion of adipose tissue cells continues. In this way, the normal digestion of adipose tissue cells can be ensured, and the digestion time is too long, which may cause certain damage and aging of the enzyme to the cells, resulting in poor cell adhesion ability and proliferation activity.

[0043] 2. The present invention calculates the difference change rate and sets a difference change rate threshold. When S is greater than or equal to the difference change rate threshold, early warning processing is performed. When S is greater than or equal to the difference change rate threshold, it indicates that the adipose tissue cells are digested too quickly per unit time and there are certain abnormalities. The adipose tissue cells need to be checked, thereby ensuring the effect of adipose tissue cell digestion.

[0044] 3. The present invention detects the activity of stem cells, and the activity rate is relatively high, which can meet the needs of stem cell preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 This is a schematic diagram of the structure of a stem cell preparation system of the present invention. DETAILED DESCRIPTION

[0046] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] Example 1

[0048] Figure 1 The present invention provides a stem cell preparation system, comprising: a cell extraction and cleaning module, a digestion module, a digestion termination module, a primary culture module, a subculture module and an activity detection module;

[0049] The cell extraction and cleaning module is used to extract adipose tissue cells and immerse the adipose tissue cells in sodium chloride injection for cleaning and disinfection;

[0050] The digestion module is used to cut the fat tissue cells into pieces, and then add digestive enzymes into the cut fat tissue cells to digest the fat tissue cells;

[0051] The digestion termination module is used for adding physiological saline into the digesting adipose tissue cells to terminate the digestion of the adipose tissue cells;

[0052] The primary culture module is used to add the digested adipose tissue cells into serum-free complete culture medium, place them in an incubator for culture, and obtain a suspension;

[0053] The subculture module is used to centrifuge the suspension and then perform subculture to obtain stem cells;

[0054] The activity detection module is used to detect the activity of stem cells.

[0055] The operation process of the cell extraction and cleaning module includes the following:

[0056] Extract 100ml of adipose tissue cells, the concentration of sodium chloride injection is 0.9%, the sodium chloride injection is 120ml, 100ml of adipose tissue cells are immersed in 120ml of 0.9% sodium chloride injection for cleaning and disinfection, the cleaning and disinfection time is set to 10min, and the adipose tissue cells are taken out after cleaning and disinfection are completed.

[0057] The operation process of the digestion module includes the following:

[0058] The fat tissue cells were cut into pieces to obtain fat tissue blocks, and the particle size of the fat tissue blocks was 2 mm. 3 The digestive enzyme is pancreatic lipase, and pancreatic lipase is added to the adipose tissue block to digest the adipose tissue cells.

[0059] The process of terminating the digestion module includes the following:

[0060] During the digestion process, the adipose tissue cells are placed under an electron microscope, which is connected to a computer. The electron microscope is controlled by the computer to record the adipose tissue cells during the digestion process.

[0061] Compare the recorded images with the images of the adipose tissue cell digestion process stored in the computer to obtain the difference rate;

[0062] The difference change rate is obtained according to the obtained difference rate.

[0063] The process of obtaining the difference rate includes the following:

[0064] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t1;

[0065] Mark the difference ratio of the adipose tissue cell digestion process photos as X i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0066] X i The difference rate is marked as M1;

[0067] The calculation formula is: In the formula, It is the average value of the difference ratio of the photos of adipose tissue cell digestion process.

[0068] The process of obtaining the difference rate also includes the following:

[0069] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t2;

[0070] The difference ratio of the adipose tissue cell digestion process photos is marked as Y i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0071] Y i The difference rate is marked as M2;

[0072] The calculation formula is: In the formula, is the average value of the difference ratio of the photos of the adipose tissue cell digestion process;

[0073] Among them, M2<M1, a difference rate threshold is set, and when M1 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is less than the difference rate threshold, the digestion of the adipose tissue cells continues.

[0074] The process of obtaining the difference change rate includes the following:

[0075] Label the difference change rate as S;

[0076] in,

[0077] Set the difference change rate threshold, and perform early warning processing when S is greater than or equal to the difference change rate threshold.

[0078] The operation process of the primary culture module includes the following:

[0079] The CO2 concentration in the incubator was set to 4% and the temperature in the incubator was set to 36°C.

[0080] The operation process of the generation training module includes the following:

[0081] When the cell confluence reaches 80%, centrifugation is performed to obtain stem cells.

[0082] Example 2

[0083] Figure 1 The present invention provides a stem cell preparation system, comprising: a cell extraction and cleaning module, a digestion module, a digestion termination module, a primary culture module, a subculture module and an activity detection module;

[0084] The cell extraction and cleaning module is used to extract adipose tissue cells and immerse the adipose tissue cells in sodium chloride injection for cleaning and disinfection;

[0085] The digestion module is used to cut the fat tissue cells into pieces, and then add digestive enzymes into the cut fat tissue cells to digest the fat tissue cells;

[0086] The digestion termination module is used for adding physiological saline into the digesting adipose tissue cells to terminate the digestion of the adipose tissue cells;

[0087] The primary culture module is used to add the digested adipose tissue cells into serum-free complete culture medium, place them in an incubator for culture, and obtain a suspension;

[0088] The subculture module is used to centrifuge the suspension and then perform subculture to obtain stem cells;

[0089] The activity detection module is used to detect the activity of stem cells.

[0090] The operation process of the cell extraction and cleaning module includes the following:

[0091] Extract 100ml of adipose tissue cells, the concentration of sodium chloride injection is 0.9%, the sodium chloride injection is 135ml, immerse 100ml of adipose tissue cells in 135ml of 0.9% sodium chloride injection for cleaning and disinfection, the cleaning and disinfection time is set to 13min, and the adipose tissue cells are taken out after cleaning and disinfection are completed.

[0092] The operation process of the digestion module includes the following:

[0093] The fat tissue cells were cut into pieces to obtain fat tissue blocks, and the particle size of the fat tissue blocks was 2.3 mm. 3 The digestive enzyme is pancreatic lipase, and pancreatic lipase is added to the adipose tissue block to digest the adipose tissue cells.

[0094] The process of terminating the digestion module includes the following:

[0095] During the digestion process, the adipose tissue cells are placed under an electron microscope, which is connected to a computer. The electron microscope is controlled by the computer to record the adipose tissue cells during the digestion process.

[0096] Compare the recorded images with the images of the adipose tissue cell digestion process stored in the computer to obtain the difference rate;

[0097] The difference change rate is obtained according to the obtained difference rate.

[0098] The process of obtaining the difference rate includes the following:

[0099] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t1;

[0100] Mark the difference ratio of the adipose tissue cell digestion process photos as X i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0101] X i The difference rate is marked as M1;

[0102] The calculation formula is: In the formula, It is the average value of the difference ratio of the photos of adipose tissue cell digestion process.

[0103] The process of obtaining the difference rate also includes the following:

[0104] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t2;

[0105] The difference ratio of the adipose tissue cell digestion process photos is marked as Y i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0106] Y i The difference rate is marked as M2;

[0107] The calculation formula is: In the formula, is the average value of the difference ratio of the photos of the adipose tissue cell digestion process;

[0108] Among them, M2<M1, a difference rate threshold is set, and when M1 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is less than the difference rate threshold, the digestion of the adipose tissue cells continues.

[0109] The process of obtaining the difference change rate includes the following:

[0110] The difference change rate is labeled as S;

[0111] in,

[0112] Set the difference change rate threshold, and perform early warning processing when S is greater than or equal to the difference change rate threshold.

[0113] The operation process of the primary culture module includes the following:

[0114] The CO2 concentration in the incubator was set to 5%, and the temperature in the incubator was set to 37°C.

[0115] The operation process of the generation training module includes the following:

[0116] When the cell confluence reached 83%, the cells were centrifuged to obtain stem cells.

[0117] Example 3

[0118] Figure 1 The present invention provides a stem cell preparation system, comprising: a cell extraction and cleaning module, a digestion module, a digestion termination module, a primary culture module, a subculture module and an activity detection module;

[0119] The cell extraction and cleaning module is used to extract adipose tissue cells and immerse the adipose tissue cells in sodium chloride injection for cleaning and disinfection;

[0120] The digestion module is used to cut the fat tissue cells into pieces, and then add digestive enzymes into the cut fat tissue cells to digest the fat tissue cells;

[0121] The digestion termination module is used for adding physiological saline into the digesting adipose tissue cells to terminate the digestion of the adipose tissue cells;

[0122] The primary culture module is used to add the digested adipose tissue cells into serum-free complete culture medium, place them in an incubator for culture, and obtain a suspension;

[0123] The subculture module is used to centrifuge the suspension and then perform subculture to obtain stem cells;

[0124] The activity detection module is used to detect the activity of stem cells.

[0125] The operation process of the cell extraction and cleaning module includes the following:

[0126] Extract 100ml of adipose tissue cells, the concentration of sodium chloride injection is 0.9%, the sodium chloride injection is 150ml, immerse 100ml of adipose tissue cells in 150ml of 0.9% sodium chloride injection for cleaning and disinfection, the cleaning and disinfection time is set to 15min, and the adipose tissue cells are taken out after cleaning and disinfection are completed.

[0127] The operation process of the digestion module includes the following:

[0128] The fat tissue cells were cut into pieces to obtain fat tissue blocks, and the particle size of the fat tissue blocks was 2.5 mm. 3 The digestive enzyme is pancreatic lipase, and pancreatic lipase is added to the adipose tissue block to digest the adipose tissue cells.

[0129] The process of terminating the digestion module includes the following:

[0130] During the digestion process, the adipose tissue cells are placed under an electron microscope, which is connected to a computer. The electron microscope is controlled by the computer to record the adipose tissue cells during the digestion process.

[0131] Compare the recorded images with the images of the adipose tissue cell digestion process stored in the computer to obtain the difference rate;

[0132] The difference change rate is obtained according to the obtained difference rate.

[0133] The process of obtaining the difference rate includes the following:

[0134] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t1;

[0135] Mark the difference ratio of the adipose tissue cell digestion process photos as X i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0136] X i The difference rate is marked as M1;

[0137] The calculation formula is: In the formula, It is the average value of the difference ratio of the photos of adipose tissue cell digestion process.

[0138] The process of obtaining the difference rate also includes the following:

[0139] Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t2;

[0140] The difference ratio of the adipose tissue cell digestion process photos is marked as Y i , i=1, 2, 3, 4, ..., n, n is a positive integer;

[0141] Y i The difference rate is marked as M2;

[0142] The calculation formula is: In the formula, is the average value of the difference ratio of the photos of the adipose tissue cell digestion process;

[0143] Among them, M2<M1, a difference rate threshold is set, and when M1 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is less than the difference rate threshold, the digestion of the adipose tissue cells continues.

[0144] The process of obtaining the difference change rate includes the following:

[0145] The difference change rate is labeled as S;

[0146] in,

[0147] Set the difference change rate threshold, and perform early warning processing when S is greater than or equal to the difference change rate threshold.

[0148] The operation process of the primary culture module includes the following:

[0149] The CO2 concentration in the incubator was set to 6% and the temperature in the incubator was set to 38°C.

[0150] The operation process of the generation training module includes the following:

[0151] When the cell confluence reaches 85%, centrifugation is performed to obtain stem cells.

[0152] The activity of the stem cells prepared in Examples 1 to 3 was tested, and the results are shown in the following table:

[0153]

[0154] It can be seen from the above table that the stem cell preparation system of the present invention can effectively improve the viability of stem cells, and Example 2 is the best example.

[0155] The above formulas are all dimensionless and numerical calculations. The formula is a formula for the most recent real situation obtained by collecting a large amount of data and performing software simulation. The preset parameters and thresholds in the formula are set by technicians in this field according to actual conditions.

[0156] The above embodiments can be implemented in whole or in part by software, hardware, firmware or any other combination. When implemented using software, the above embodiments can be implemented in whole or in part in the form of a computer program product. The computer program product includes one or more computer instructions or computer programs. When the computer instructions or computer programs are loaded or executed on a computer, the process or function described in the embodiment of the present application is generated in whole or in part. The computer can be a general-purpose computer, a special-purpose computer, a computer network, or other programmable devices. The computer instructions can be stored in a computer-readable storage medium, or transmitted from one computer-readable storage medium to another computer-readable storage medium. For example, the computer instructions can be transmitted from one website site, computer, server or data center to another website site, computer, server or data center by wired (e.g., infrared, wireless, microwave, etc.). The computer-readable storage medium can be any available medium that a computer can access or a data storage device such as a server or data center that contains one or more available media sets. The available medium can be a magnetic medium (e.g., a floppy disk, a hard disk, a tape), an optical medium (e.g., a DVD), or a semiconductor medium. The semiconductor medium can be a solid-state hard disk.

[0157] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0158] In the several embodiments provided in the present application, it should be understood that the disclosed systems and devices can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interfaces, devices or units, which can be electrical, mechanical or other forms.

[0159] The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0160] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit.

[0161] If the functions are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present application can be essentially or partly embodied in the form of a software product that contributes to the prior art. The computer software product is stored in a storage medium and includes several instructions for a computer device (which can be a personal computer, server, or network device, etc.) to perform all or part of the steps described in each embodiment of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM), random access memory (RAM), magnetic disk or optical disk, and other media that can store program codes.

[0162] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

[0163] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A stem cell preparation system, characterized in that: include: Cell extraction and cleaning module, digestion module, digestion termination module, primary culture module, subculture module and activity detection module; The cell extraction and cleaning module is used to extract adipose tissue cells and immerse the adipose tissue cells in sodium chloride injection for cleaning and disinfection; The digestion module is used to cut the fat tissue cells into pieces, and then add digestive enzymes into the cut fat tissue cells to digest the fat tissue cells; The digestion termination module is used for adding physiological saline into the digesting adipose tissue cells to terminate the digestion of the adipose tissue cells; The primary culture module is used to add the digested adipose tissue cells into serum-free complete culture medium, place them in an incubator for culture, and obtain a suspension; The subculture module is used to centrifuge the suspension and then perform subculture to obtain stem cells; The activity detection module is used to detect the activity of stem cells.

2. A stem cell preparation system according to claim 1, characterized in that: The operation process of the cell extraction and cleaning module includes the following: Extract 100ml of adipose tissue cells, the concentration of sodium chloride injection is 0.9%, the sodium chloride injection is 120-150ml, immerse 100ml of adipose tissue cells in 120-150ml of 0.9% sodium chloride injection for cleaning and disinfection, the cleaning and disinfection time is set to 10-15min, and the adipose tissue cells are taken out after cleaning and disinfection are completed.

3. A stem cell preparation system according to claim 1, characterized in that: The operation process of the digestion module includes the following: The fat tissue cells are cut into pieces to obtain fat tissue blocks, and the particle size of the fat tissue blocks is 2 to 2.5 mm. 3 The digestive enzyme is pancreatic lipase, and pancreatic lipase is added to the adipose tissue block to digest the adipose tissue cells.

4. A stem cell preparation system according to claim 1, characterized in that: The process of terminating the digestion module includes the following: During the digestion process, the adipose tissue cells are placed under an electron microscope, which is connected to a computer. The electron microscope is controlled by the computer to record the adipose tissue cells during the digestion process. Compare the recorded images with the images of the adipose tissue cell digestion process stored in the computer to obtain the difference rate; The difference change rate is obtained according to the obtained difference rate.

5. A stem cell preparation system according to claim 4, characterized in that: The process of obtaining the difference rate includes the following: Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t1; Mark the difference ratio of the adipose tissue cell digestion process photos as X i , i=1, 2, 3, 4, ..., n, n is a positive integer; X i The difference rate is marked as M1; The calculation formula is: In the formula, It is the average value of the difference ratio of the photos of adipose tissue cell digestion process.

6. A stem cell preparation system according to claim 5, characterized in that: The process of obtaining the difference rate also includes the following: Record the difference ratio of the photos of the adipose tissue cell digestion process taken at time t2; The difference ratio of the adipose tissue cell digestion process photos is marked as Y i , i=1, 2, 3, 4, ..., n, n is a positive integer; Y i The difference rate is marked as M2; The calculation formula is: In the formula, is the average value of the difference ratio of the photos of the adipose tissue cell digestion process; Among them, M2<M1, a difference rate threshold is set, and when M1 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is between the difference rate threshold, the digestion of the adipose tissue cells is terminated; when M2 is less than the difference rate threshold, the digestion of the adipose tissue cells continues.

7. A stem cell preparation system according to claim 6, characterized in that: The process of obtaining the difference change rate includes the following: Label the difference change rate as S; in, Set the difference change rate threshold, and perform early warning processing when S is greater than or equal to the difference change rate threshold.

8. A stem cell preparation system according to claim 1, characterized in that: The operation process of the primary culture module includes the following: The CO2 concentration in the incubator was set to 4-6%, and the temperature in the incubator was set to 36-38°C.

9. A stem cell preparation system according to claim 1, characterized in that: The operation process of the subculture module includes the following: When the cell confluence reaches 80-85%, centrifugation is performed to obtain stem cells.

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