Plant cell group solid culture device and culture method thereof
By designing a solid culture device for plant cell groups that includes multiple collaborative work, the problem of uneven distribution of plant tissue cells in a single culture is solved, and more efficient cell culture and uniform distribution is achieved.
Patent Information
- Application Number
- CN202411793391.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-02-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing plant tissue cell solid culture device has uneven distribution of plant tissue cells in a single culture, resulting in low culture efficiency.
A solid culture device for plant cell group is designed, including a barrel, a longitudinal rotation shaft, a directional rotation mechanism, a liquid supply system, a floating mixing mechanism, an elastic adjustment mechanism and a circumferential driving mechanism. Through the coordinated work of these components, uniform distribution of plant tissue cells and culture fluid is achieved.
This device can effectively avoid the problem of concentration of plant tissue cells in the culture medium, ensure the uniform distribution of cells and culture medium, improve the culture efficiency of plant tissue cells, and support the culture conditions for stable absorption of light.
Smart Images
Figure CN119506090A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of plant tissue cells, and in particular relates to a plant cell group solid culture device and a culture method thereof. Background Art
[0002] The currently used solid culture device for plant tissue cells is equipped with a shaking mechanism. The external control mechanism starts the motor, and the motor drives the connecting rod assembly to rotate, thereby causing the culture mechanism connected by the connecting rod assembly to rotate as a whole, shaking the culture solution placed in the solid culture device, thereby solving the problem of inconvenience in adding nutrient solution to the solid culture device.
[0003] However, the number of plant tissue cells cultured at a single time is limited, which is not conducive to improving the efficiency of plant tissue cell culture. In addition, when mixed by shaking, the culture solution and the plant tissue cells cannot be evenly distributed, resulting in a concentration problem, which is not conducive to subsequent culture work. Summary of the invention
[0004] The purpose of the embodiments of the present invention is to provide a plant cell group solid culture device and a culture method thereof, aiming to solve the problems existing in the above-mentioned background technology.
[0005] The embodiment of the present invention is implemented as follows: a plant cell group solid culture device includes a barrel, a longitudinal rotating shaft, a directional rotating mechanism, a liquid supply system, a floating mixing mechanism, an elastic adjustment mechanism and a circumferential driving mechanism; The longitudinal rotating shaft 1 is rotatably mounted on the barrel body, and a double-track pulley is installed at the end of the longitudinal rotating shaft 1 and is driven by an external motor; The directional rotation mechanism is arranged at the end of the longitudinal rotation shaft 1, is used to place plant cell tissue, and rotates synchronously with the longitudinal rotation shaft 1; The liquid supply system is installed on the outside of the barrel to add culture liquid to the plant tissue cells; The floating mixing mechanism is installed on the barrel body so as to facilitate mixing when the placement frame in the directional rotating mechanism is directly below the floating mixing mechanism; The elastic adjustment mechanism is arranged on the barrel body, the elastic adjustment mechanism is in transmission connection with the longitudinal rotating shaft, and the elastic adjustment mechanism is also connected with the floating mixing mechanism to facilitate height adjustment during mixing; The circumferential driving mechanism is arranged at the bottom end of the barrel body and is driven by an external rotating motor to adjust the rotation angle of the barrel body.
[0006] Preferably, the directional rotation mechanism comprises a fixing rod, a square frame, a positioning column and a placement frame; The fixing rods are provided in plurality and fixedly connected to the end of the longitudinal rotating shaft 1, and a positioning column is rotatably mounted on the end of each fixing rod; The end of the positioning column is eccentrically installed with the square frame, and a placement frame is movably installed inside the square frame.
[0007] Preferably, the liquid supply system comprises a fixing plate, a liquid storage tank, a diagonal support rod, a liquid infusion tube and a nozzle; The fixing plate is horizontally installed on the outside of the barrel, and a liquid storage tank is installed at the bottom of the fixing plate; A liquid storage tank is installed on the upper surface of the fixed plate, a side of the liquid storage tank is connected with a liquid infusion pipe, and a nozzle is longitudinally arranged at the end of the liquid infusion pipe.
[0008] Preferably, the floating mixing mechanism comprises a bracket, a second longitudinal rotating shaft, a transmission shaft, a first bevel gear, a second bevel gear, a stirring shaft and a stirring rod; The bracket is fixedly mounted on the end of the barrel body, a second longitudinal rotating shaft is rotatably mounted on the bracket, and a first bevel gear is mounted on the end of the second longitudinal rotating shaft; The other end of the second longitudinal rotating shaft is provided with a single track pulley, which is connected to the double track pulley through a synchronous belt. The transmission shaft is rotatably mounted on the barrel body, and a second bevel gear is mounted on the transmission shaft, and the second bevel gear is meshed with the first bevel gear; A stirring shaft is slidably installed in the transmission shaft, and a stirring rod is installed at the bottom end of the stirring shaft to stir the culture solution.
[0009] Preferably, the elastic adjustment mechanism comprises a base, a longitudinal rotating shaft 3, a cam, a movable rod, a column and an elastic support member; The base is fixedly mounted on the barrel body, and a longitudinal rotating shaft 3 is rotatably mounted on the base, and a single track pulley at the end of the longitudinal rotating shaft 3 is transmission-connected to the longitudinal rotating shaft 1 through a synchronous belt; A cam is installed on the longitudinal rotating shaft 3, the cam abuts against the movable rod, the end of the movable rod is rotatably connected to the top of the stirring shaft, and the limit bar installed on the stirring shaft is slidably connected to the transmission shaft; The movable rod is slidably connected with a column arranged on the side of the barrel body, and an elastic supporting piece is sleeved on the column.
[0010] Preferably, the circumferential driving mechanism comprises a base plate, a support rod, an annular groove and a gear shaft; The bottom plate is arranged at the bottom end of the barrel body, the support rod is fixedly installed on the side of the barrel body, and the support rod is slidably connected with the annular groove provided in the bottom plate; The interior of the bottom plate is a hollow structure, in which an outer gear ring connected to the support rod is arranged, and the outer gear ring is also in a meshing state with the gear shaft installed on the bottom plate.
[0011] A plant cell group solid culture method comprises the following steps: S1, the directional rotation mechanism drives the boxes and placement boxes at multiple positions to rotate, and when the boxes rotate to a horizontal position, the culture solution is added to the inside of the boxes through the liquid supply system; S2, the longitudinal rotating shaft 1 drives the floating mixing mechanism through the synchronous belt to stir the culture solution in the placement frame; S3, the longitudinal rotating shaft 1 also drives the elastic adjustment mechanism to rise and fall through the synchronous belt, and pulls the floating mixing mechanism to move synchronously, so as to adjust the mixing height regularly; S4, the barrel and the multiple placement frames inside can also be rotated by the arranged circumferential drive mechanism The beneficial effects of the present invention are that the placement frame is not only easy to load and unload, but also easy to add culture solution into the interior. After adding, it can be fully stirred while avoiding movement interference, ensuring that the culture solution and plant tissue cells in each placement frame are evenly distributed. In addition, the movement trajectory of the placement frame can also be controlled to ensure stable absorption of light for cultivation. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 A schematic diagram of the structure of a plant cell group solid culture device provided by an embodiment of the present invention; Figure 2 A three-dimensional structural diagram of a square frame and a placement frame in a plant cell group solid culture device provided by an embodiment of the present invention; Figure 3 for Figure 1 A partial enlarged view of the middle part; Figure 4 for Figure 1 A partial enlarged view of point B in the middle; Figure 5 A schematic diagram of the transmission of the longitudinal rotating shaft 1, the longitudinal rotating shaft 2 and the longitudinal rotating shaft 3 in a plant cell group solid culture device provided by an embodiment of the present invention; Figure 6 A schematic diagram of the structure of an end cover in a plant cell group solid culture device provided by an embodiment of the present invention; In the attached drawings: 1-barrel; 2-longitudinal rotation axis 1; 3-fixing rod; 4-square frame; 5-positioning column; 6-placing frame; 7-fixing plate; 8-liquid storage tank; 9-diagonal support rod; 10-infusion tube; 11-sprinkler; 12-bracket; 13-longitudinal rotation axis 2; 14-transmission shaft; 15-bevel gear 1; 16-bevel gear 2; 17-stirring shaft; 18-stirring rod; 19-base; 20-longitudinal rotation axis 3; 21-cam; 22-movable rod; 23-column; 24-elastic support member; 25-limiting strip; 26-double-track pulley; 27-single-track pulley; 28-synchronous belt; 29-bottom plate; 30-support rod; 31-annular groove; 32-gear shaft; 33-groove; 34-stuck column; 35-end cover; 36-handle; 100-directional rotation mechanism; 200-liquid supply system; 300-floating mixing mechanism; 400-elastic adjustment mechanism; 500-circumferential drive mechanism. DETAILED DESCRIPTION
[0013] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0014] The specific implementation of the present invention is described in detail below in conjunction with specific embodiments.
[0015] like Figure 1-Figure 6 As shown, it is a structural diagram of a plant cell group solid culture device provided by an embodiment of the present invention, comprising a barrel body 1, a longitudinal rotating shaft 2, a directional rotating mechanism 100, a liquid supply system 200, a floating mixing mechanism 300, an elastic adjustment mechanism 400 and a circumferential driving mechanism 500; the longitudinal rotating shaft 2 is rotatably mounted on the barrel body 1, and a double-track pulley 26 is installed at the end of the longitudinal rotating shaft 2 and is driven by an external motor; the directional rotating mechanism 100 is arranged at the end of the longitudinal rotating shaft 2, for placing plant cell tissues, and rotates synchronously with the longitudinal rotating shaft 2; the liquid supply system 200 is installed in the barrel body 1, and the liquid supply system 200 is installed in the barrel body 1; The outer side of the barrel body 1 is used to add culture fluid to the plant tissue cells; the floating mixing mechanism 300 is installed on the barrel body 1, so that mixing can be performed when the placement frame 6 in the directional rotation mechanism 100 is directly below the floating mixing mechanism 300; the elastic adjustment mechanism 400 is arranged on the barrel body 1, and the elastic adjustment mechanism 400 is transmission-connected with the longitudinal rotating shaft 2, and the elastic adjustment mechanism 400 is also connected to the floating mixing mechanism 300, so as to adjust the height during mixing; the circumferential driving mechanism 500 is arranged at the bottom end of the barrel body 1, and the circumferential driving mechanism 500 is driven by an external rotating motor to adjust the rotation angle of the barrel body 1.
[0016] In one example of the present invention, an end cover 35 is arranged on the side of the barrel body 1, and a plurality of clamping columns 34 are installed on the side of the end cover 35. The clamping columns 34 are slidably connected to the grooves 33 opened on the barrel body 1, and a handle 36 is installed on the outer side of the end cover 35 to facilitate the quick installation of the end cover 35 on the barrel body 1; in addition, the barrel body 1 is made of transparent material to facilitate light transmission.
[0017] like Figure 1 and Figure 2 As shown, as a preferred embodiment of the present invention, the directional rotation mechanism 100 includes a fixing rod 3, a square frame 4, a positioning column 5 and a placement frame 6; The fixing rods 3 are provided in plurality and fixedly connected to the ends of the longitudinal rotating shaft 2, and a positioning column 5 is rotatably mounted on the ends of each fixing rod 3; The end of the positioning column 5 is eccentrically mounted to the square frame 4 , and a placement frame 6 is movably mounted inside the square frame 4 .
[0018] In an example of the present invention, when the longitudinal rotating shaft 2 rotates along the barrel body 1 driven by an external motor, the fixing rod 3 rotates synchronously therewith, and the plurality of position frames 4 and the placement frame 6 move horizontally inside the barrel body 1 accordingly.
[0019] like Figure 1 As shown, as another preferred embodiment of the present invention, the liquid supply system 200 includes a fixing plate 7, a liquid storage tank 8, a diagonal support rod 9, a liquid infusion tube 10 and a nozzle 11; The fixing plate 7 is horizontally installed on the outside of the barrel body 1, and a liquid storage tank 8 is installed at the bottom of the fixing plate 7; A liquid storage tank 8 is installed on the upper surface of the fixing plate 7 , a liquid infusion tube 10 is connected to the side of the liquid storage tank 8 , and a nozzle 11 is longitudinally arranged at the end of the liquid infusion tube 10 .
[0020] In one example of the present invention, when the square frame 4 and the placement frame 6 are rotated to a horizontal state, the pump body inside the liquid storage tank 8 works, the culture fluid enters the barrel body 1 along the infusion tube 10, and is finally sprayed from the nozzle 11 into the placement frame 6.
[0021] like Figure 1 , Figure 3 and Figure 5 As shown, as another preferred embodiment of the present invention, the floating mixing mechanism 300 includes a bracket 12, a longitudinal rotating shaft 2 13, a transmission shaft 14, a bevel gear 1 15, a bevel gear 2 16, a stirring shaft 17 and a stirring rod 18; The bracket 12 is fixedly mounted on the end of the barrel body 1, and a longitudinal rotating shaft 13 is rotatably mounted on the bracket 12, and a bevel gear 15 is mounted on the end of the longitudinal rotating shaft 13; The other end of the second longitudinal rotating shaft 13 is provided with a single track pulley 27, which is connected to the double track pulley 26 through a synchronous belt 28; The transmission shaft 14 is rotatably mounted on the barrel body 1, and a second bevel gear 16 is mounted on the transmission shaft 14, and the second bevel gear 16 is meshed with the first bevel gear 15; A stirring shaft 17 is slidably mounted in the transmission shaft 14 , and a stirring rod 18 is mounted at the bottom end of the stirring shaft 17 to stir the culture solution.
[0022] In one example of the present invention, when the longitudinal rotating shaft 1 2 rotates, the double-track pulley 26 rotates synchronously, and drives the single-track pulley 27 and the longitudinal rotating shaft 2 13 to rotate through the arranged synchronous belt 28. Since the bevel gear 1 15 and the bevel gear 2 16 are in a meshing state, the transmission shaft 14 can be driven to rotate along the barrel body 1 when the longitudinal rotating shaft 2 13 rotates. At the same time, the stirring shaft 17 and the stirring rod 18 rotate synchronously therewith to stir the culture medium in the placement frame 6.
[0023] like Figure 1 , Figure 4 and Figure 5 As shown, as another preferred embodiment of the present invention, the elastic adjustment mechanism 400 includes a base 19, a longitudinal rotation shaft 20, a cam 21, a movable rod 22, a column 23 and an elastic support member 24; The base 19 is fixedly mounted on the barrel body 1, and a longitudinal rotating shaft 3 20 is rotatably mounted on the base 19, and a single track pulley 27 at the end of the longitudinal rotating shaft 3 20 is transmission-connected to the longitudinal rotating shaft 1 2 through a synchronous belt 28; The longitudinal rotating shaft 20 is provided with a cam 21, the cam 21 abuts against a movable rod 22, the end of the movable rod 22 is rotatably connected to the top of the stirring shaft 17, and a limit bar 25 installed on the stirring shaft 17 is slidably connected to the transmission shaft 14; The movable rod 22 is slidably connected to a column 23 arranged on the side of the barrel body 1 , and an elastic support member 24 is sleeved on the column 23 .
[0024] In one example of the present invention, the longitudinal rotating shaft 1 2 drives the longitudinal rotating shaft 3 20 and the cam 21 to rotate along the base 19 through the synchronous belt 28. The cam 21 pushes the movable rod 22 to slide along the column 23. The elastic support member 24 is elastically deformed by the force during this process. The movable rod 22 can pull the stirring shaft 17 to move in the height direction to avoid motion interference when the frame 4 and the placement frame 6 move horizontally.
[0025] like Figure 1 As shown, as another preferred embodiment of the present invention, the circumferential driving mechanism 500 includes a bottom plate 29, a support rod 30, an annular groove 31 and a gear shaft 32; The bottom plate 29 is arranged at the bottom end of the barrel body 1, and the support rod 30 is fixedly installed on the side of the barrel body 1, and the support rod 30 is slidably connected with the annular groove 31 opened on the bottom plate 29; The interior of the bottom plate 29 is a hollow structure, and an outer gear ring connected to the support rod 30 is arranged inside. The outer gear ring is also in meshing state with the gear shaft 32 installed on the bottom plate 29.
[0026] In one embodiment of the present invention, when the rotation angle of the barrel body 1 and the internal mechanism needs to be adjusted, the external motor can drive the gear shaft 32 to rotate along the bottom plate 29. Since the gear shaft 32 is engaged with the outer gear ring, the outer gear ring and the support rod 30 can be driven to slide synchronously along the annular groove 31 opened on the bottom plate 29, thereby driving the barrel body 1 and the internal components to rotate.
[0027] A plant cell group solid culture method comprises the following detailed steps: S1, when the longitudinal rotating shaft 1 2 is driven by the external motor to rotate along the barrel body 1, the fixing rod 3 rotates synchronously therewith, and the plurality of position frames 4 and the placement frame 6 move horizontally inside the barrel body 1; S2, when the square frame 4 and the placement frame 6 are rotated to a horizontal state, the pump body inside the liquid storage tank 8 works, the culture solution enters the barrel body 1 along the infusion tube 10, and is finally sprayed from the nozzle 11 into the placement frame 6; S3, when the longitudinal rotating shaft 1 2 rotates, the single track pulley 27 and the longitudinal rotating shaft 2 13 are driven to rotate through the synchronous belt 28. Since the bevel gear 1 15 and the bevel gear 2 16 are in a meshing state, the transmission shaft 14 can be driven to rotate along the barrel body 1 when the longitudinal rotating shaft 2 13 rotates. At the same time, the stirring shaft 17 and the stirring rod 18 rotate synchronously therewith to stir the culture solution in the placement frame 6; S4, the longitudinal rotating shaft 1 2 drives the longitudinal rotating shaft 3 20 and the cam 21 to rotate along the base 19 through the synchronous belt 28, and the cam 21 pushes the movable rod 22 to slide along the column 23, and the movable rod 22 can pull the stirring shaft 17 to move in the height direction; S5, the external motor can drive the gear shaft 32 to rotate along the bottom plate 29. Since the gear shaft 32 is meshed with the outer gear ring, it can drive the outer gear ring and the support rod 30 to slide synchronously along the annular groove 31 opened on the bottom plate 29, thereby driving the barrel body 1 and the internal components to rotate.
[0028] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.
[0029] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.
Claims
1. A plant cell group solid culture device, characterized in that: It includes a barrel, a longitudinal rotating shaft, a directional rotating mechanism, a liquid supply system, a floating mixing mechanism, an elastic adjusting mechanism and a circumferential driving mechanism; The longitudinal rotating shaft 1 is rotatably mounted on the barrel body, and a double-track pulley is installed at the end of the longitudinal rotating shaft 1 and is driven by an external motor; The directional rotation mechanism is arranged at the end of the longitudinal rotation shaft 1, is used to place plant cell tissue, and rotates synchronously with the longitudinal rotation shaft 1; The liquid supply system is installed on the outside of the barrel to add culture liquid to the plant tissue cells; The floating mixing mechanism is installed on the barrel body so as to facilitate mixing when the placement frame in the directional rotating mechanism is directly below the floating mixing mechanism; The elastic adjustment mechanism is arranged on the barrel body, the elastic adjustment mechanism is in transmission connection with the longitudinal rotating shaft, and the elastic adjustment mechanism is also connected with the floating mixing mechanism to facilitate height adjustment during mixing; The circumferential driving mechanism is arranged at the bottom end of the barrel body and is driven by an external rotating motor to adjust the rotation angle of the barrel body.
2. A plant cell group solid culture device according to claim 1, characterized in that: The directional rotation mechanism includes a fixing rod, a square frame, a positioning column and a placement frame; The fixing rods are provided in plurality and fixedly connected to the end of the longitudinal rotating shaft 1, and a positioning column is rotatably mounted on the end of each fixing rod; The end of the positioning column is eccentrically installed with the square frame, and a placement frame is movably installed inside the square frame.
3. A plant cell group solid culture device according to claim 1, characterized in that: The liquid supply system comprises a fixed plate, a liquid storage tank, a diagonal support rod, a liquid infusion tube and a nozzle; The fixing plate is horizontally installed on the outside of the barrel, and a liquid storage tank is installed at the bottom of the fixing plate; A liquid storage tank is installed on the upper surface of the fixed plate, a side of the liquid storage tank is connected with a liquid infusion pipe, and a nozzle is longitudinally arranged at the end of the liquid infusion pipe.
4. The plant cell group solid culture device according to claim 1, characterized in that: The floating mixing mechanism comprises a bracket, a second longitudinal rotating shaft, a transmission shaft, a first bevel gear, a second bevel gear, a stirring shaft and a stirring rod; The bracket is fixedly mounted on the end of the barrel body, a second longitudinal rotating shaft is rotatably mounted on the bracket, and a first bevel gear is mounted on the end of the second longitudinal rotating shaft; The other end of the second longitudinal rotating shaft is provided with a single track pulley, which is connected to the double track pulley through a synchronous belt. The transmission shaft is rotatably mounted on the barrel body, and a second bevel gear is mounted on the transmission shaft, and the second bevel gear is meshed with the first bevel gear; A stirring shaft is slidably installed in the transmission shaft, and a stirring rod is installed at the bottom end of the stirring shaft to stir the culture solution.
5. A plant cell group solid culture device according to claim 4, characterized in that: The elastic adjustment mechanism comprises a base, a longitudinal rotating shaft 3, a cam, a movable rod, a column and an elastic support member; The base is fixedly mounted on the barrel body, and a longitudinal rotating shaft 3 is rotatably mounted on the base, and a single track pulley at the end of the longitudinal rotating shaft 3 is transmission-connected to the longitudinal rotating shaft 1 through a synchronous belt; A cam is installed on the longitudinal rotating shaft 3, the cam abuts against the movable rod, the end of the movable rod is rotatably connected to the top of the stirring shaft, and the limit bar installed on the stirring shaft is slidably connected to the transmission shaft; The movable rod is slidably connected with a column arranged on the side of the barrel body, and an elastic supporting piece is sleeved on the column.
6. The plant cell group solid culture device according to claim 1, characterized in that: The circumferential driving mechanism comprises a base plate, a support rod, an annular groove and a gear shaft; The bottom plate is arranged at the bottom end of the barrel body, the support rod is fixedly installed on the side of the barrel body, and the support rod is slidably connected with the annular groove provided in the bottom plate; The interior of the bottom plate is a hollow structure, in which an outer gear ring connected to the support rod is arranged, and the outer gear ring is also in a meshing state with the gear shaft installed on the bottom plate.
7. A method for solid culture of plant cells, characterized in that: Cultivating using the plant cell group solid culture device according to any one of claims 1 to 6; The plant cell tissue culture method comprises the following steps: S1, the directional rotation mechanism drives the boxes and placement boxes at multiple positions to rotate, and when the boxes rotate to a horizontal position, the culture solution is added to the inside of the boxes through the liquid supply system; S2, the longitudinal rotating shaft 1 drives the floating mixing mechanism through the synchronous belt to stir the culture solution in the placement frame; S3, the longitudinal rotating shaft 1 also drives the elastic adjustment mechanism to rise and fall through the synchronous belt, and pulls the floating mixing mechanism to move synchronously, so as to adjust the mixing height regularly; S4, the barrel body and the multiple placement frames inside can also be rotated by the arranged circumferential driving mechanism.