Breast enlarging composition containing natural plant extract and preparation method thereof

Abstract

The invention relates to the technical field of daily necessities, and particularly discloses a breast enlarging composition containing natural plant extracts and a preparation method of the breast enlarging composition. The breast enlargement composition containing the natural plant extracts comprises the following components: a CYNARA SCOLYMUS leaf extract, a HUMULUS LUPULUS extract, a puerarin root extract, a pawpaw fruit extract, a ginseng root extract, acetyl hexapeptide-8, hexapeptide-9, dipeptide diaminobutyrylbenzylamide diacetate, arginine / lysine polypeptide and deionized water, and is prepared from the following raw materials: the CYNARA SCOLYMUS leaf extract, the HUMULUS LUPULUS extract, the puerarin root extract, the pawpaw fruit extract, the PANAX GINSENG root extract, the acetyl hexapeptide-8, the hexapeptide-9, the dipeptide diaminobutyrylbenzylamide diacetate, the arginine / lysine polypeptide and the deionized water. Through the synergistic effect of all the components, breast tissue development and cell regeneration are further promoted, so that the compactness of breast skin is enhanced, and the breast enlarging effect is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of daily necessities and relates to a breast enlargement composition containing natural plant extracts and a preparation method thereof. Background Art

[0002] Breasts are an important part of the female body. With the changes of the times, women's pursuit of beautiful body curves is also increasing. Due to incomplete breast development in adolescence, inadequate care after breastfeeding, or uneven estrogen secretion before and after menopause, many women are troubled by problems such as small, flat, loose, and sagging breasts.

[0003] There are many kinds of breast enhancement products on the market, including breast enhancement creams, breast enhancement essential oils, etc. Most of them are artificial synthetic hormones with irritating ingredients and poor efficacy. Long-term use may cause irritation to the skin and cause health problems. Summary of the invention

[0004] The object of the present invention is to provide a breast enhancement composition containing natural plant extracts and a preparation method thereof. The present invention further promotes breast tissue development and cell regeneration through the synergistic effect of artichoke (CYNARA SCOLYMUS) leaf extract, hops (HUMULUS LUPULUS) extract, kudzu (PUERARIA LOBATA) root extract, papaya (CHAENOMELES SINENSIS) fruit extract, ginseng (PANAXGINSENG) root extract, acetyl hexapeptide-8, hexapeptide-9, dipeptide diaminobutyryl benzylamide diacetate, and arginine / lysine polypeptide, thereby enhancing the firmness of breast skin and achieving breast enhancement effect.

[0005] The purpose of the present invention can be achieved through the following technical solutions: A breast enlargement composition containing natural plant extracts, wherein the raw materials of the breast enlargement composition include, by weight, 0.8-1.2 parts of cynarin (cynarinus scolymus) leaf extract, 19-21 parts of hop (humulus lupulus) extract, 1.5-2.5 parts of pueraria lobata (pueraria lobata) root extract, 0.3-0.7 parts of papaya (chaenomeles sinenessis) fruit extract, 18-22 parts of ginseng (panax ginseng) root extract, 1.4-1.6 parts of acetyl hexapeptide-8, 1.2-1.3 parts of hexapeptide-9, 1.2-1.4 parts of dipeptide diaminobutyryl benzylamide diacetate, 1.3-1.5 parts of arginine / lysine polypeptide and 40-50 parts of deionized water; The preparation method of the artichoke (CYNARA SCOLYMUS) leaf extract and the hop (HUMULUS LUPULUS) extract comprises the following steps: Step S1, washing and drying safflower, adding it into deionized water at a ratio of 1:8-12 g / ml, adding Bacillus subtilis accounting for 0.1-0.2% of the mass of safflower, and simultaneously adding 0.1-0.3 mol / L citric acid dropwise to adjust the pH to 6.5-7.5, fermenting at 30-40° C. for 3-7 days, filtering with a 300-mesh filter cloth after the fermentation is completed, and obtaining safflower fermentation liquid; Step S2, mixing safflower fermentation liquid, soybean extract, Tween-80 and deionized water in a mass ratio of 1:0.5-1.1:0.2-0.3:5-9, and adding 0.1-0.3 mol / L citric acid to adjust the pH to 6.8-7.2, and stirring to obtain fermentation liquid; Step S3, washing and chopping the artichoke leaves, adding them to the fermentation liquid at a ratio of 1:5-7 g / ml, adding Bacillus subtilis accounting for 0.12-0.18% of the mass of the artichoke leaves, fermenting at 32-34° C. for 3-5 days to obtain artichoke leaf fermentation products, filtering with a 300-mesh filter cloth to obtain artichoke leaf fermentation clear liquid, mixing the artichoke leaf fermentation clear liquid and olive oil at a volume ratio of 1:1.4-1.8, soaking for 24-28 hours, and freeze-drying to obtain artichoke (CYNARA SCOLYMUS) leaf extract; Step S4, washing and crushing the hops, passing through a 60-100 mesh sieve, adding the hops to the fermentation liquid at a ratio of 1:6-6.8 g / ml, adding Bacillus subtilis accounting for 0.2-0.22% of the mass of the hops, fermenting at 35-37° C. for 3-5 days to obtain a hop fermentation product, filtering with a 300-mesh filter cloth to obtain a hop fermentation clear liquid, mixing the hop fermentation clear liquid and olive oil at a volume ratio of 1:1.6-2, soaking for 26-32 hours, and freeze-drying to obtain a hop (HUMULUS LUPULUS) extract.

[0006] Furthermore, the effective bacterial content of the Bacillus subtilis is 1.1-1.3×10 7 CFU / g; the freeze-drying operation is to pre-freeze at -40°C for 4-6 hours, and then heat to 10°C at a heating rate of 1-2°C / h under a pressure of 120-160μmHg.

[0007] Furthermore, the preparation method of the breast enhancement composition comprises the following steps: Step 1, adding artichoke (CYNARA SCOLYMUS) leaf extract, hops (HUMULUS LUPULUS) extract, kudzu (PUERARIA LOBATA) root extract, papaya (CHAENOMELES SINENSIS) fruit extract, and ginseng (PANAXGINSENG) root extract into deionized water, and mixing by ultrasonication to obtain a mixture; Step 2: Add acetyl hexapeptide-8 and hexapeptide-9 to preheated deionized water, stir at medium speed for 10-20 minutes, then add dipeptide diaminobutyryl benzylamide diacetate, stir at medium speed for 5-10 minutes, then add arginine / lysine polypeptide, stir at medium speed for 4-10 minutes, transfer the obtained mixed solution to a high shear emulsifier for staged emulsification, adjust the pH at the same time, filter, and obtain 4D anti-wrinkle composite peptide; Step 3: Add 4D anti-wrinkle complex peptide into the mixture in a high-pressure shear emulsifier and start the high-pressure shear emulsifier to process to obtain a breast enhancement composition.

[0008] Furthermore, the parameters of the ultrasonic mixing in step 1 are: ultrasonic frequency of 40-60kHz, ultrasonic frequency of 100-500W, ultrasonic temperature of 38-46°C, and ultrasonic time of 10-20min.

[0009] Furthermore, the mass ratio of the deionized water in step 1 and step 2 is 2-3:1; and the temperature of the preheated deionized water in step 2 is 50-60°C.

[0010] Furthermore, the speed of the medium-speed stirring in step 2 is 300-400rpm; the segmented emulsification is first stirred at a speed of 2000-4000rpm for 2-8min, and then stirred at a speed of 6000-10000rpm for 15-25min; the pH adjustment refers to adjusting the pH to 5.0-6.5 using 0.15-0.25mol / L citric acid; and the filtration uses a 0.22μm filter membrane.

[0011] Furthermore, the rotation speed and time of the high-pressure shear emulsifier treatment in step 3 are 3000-6000 rpm and 10-20 min respectively.

[0012] Furthermore, the breast enhancement composition is added in an amount of 7.5-8.5wt% in a breast enhancement product.

[0013] Beneficial effects of the present invention: (1) The artichoke (CYNARA SCOLYMUS) leaf extract added in the present invention is rich in polyphenols and flavonoids, has antioxidant and microcirculation-promoting effects, can help improve skin elasticity and firmness, and can also soothe the skin and increase skin moisture; the hops (HUMULUS LUPULUS) extract contains phytoestrogens, which can simulate the effects of estrogen in the body, thereby promoting the development and fullness of breast tissue; the kudzu (PUERARIA LOBATA) root extract is rich in phytoestrogens, which has a promoting effect on the growth of breast cells and further enhances the breast enhancement effect; the papaya (CHAENOMELES SINENSIS) fruit extract is rich in vitamins and enzymes, which helps promote the metabolism and cell regeneration of breast tissue; the ginseng (PANAXGINSENG) root extract has anti-aging and cell vitality-enhancing effects, can stimulate the healthy development of breast tissue, and support the breast fullness effect. Multiple plant extracts synergistically exert the breast enhancement effect.

[0014] (2) The present invention uses a fermentation broth containing safflower fermentation broth and soybean extract when preparing artichoke (CYNARA SCOLYMUS) leaf extract and hop (HUMULUS LUPULUS) extract. With the help of the fermentation action of Bacillus subtilis, the complex macromolecules in the plant raw materials are converted into small molecules that are more easily absorbed, so that the prepared products are more easily absorbed by the skin, significantly improving the permeability and bioavailability of the extracts. In addition, safflower fermentation broth improves microcirculation and anti-oxidation, soybean extract provides additional phytoestrogens, and finally olive oil is used to optimize the absorption of active ingredients. The components work synergistically to effectively promote the development of breast tissue, improve skin elasticity, and ultimately achieve a breast enhancement effect.

[0015] (3) The present invention also adds 4D anti-wrinkle composite peptides, wherein acetyl hexapeptide-8 blocks the release of neurotransmitters, reduces the frequency of muscle contraction, relaxes the surface muscles of the skin around the breast, and makes the skin around the breast smoother and firmer; hexapeptide-9 can stimulate fibroblasts to synthesize collagen and elastin, promote the regeneration ability of breast skin tissue, and make the breasts appear fuller and rounder; dipeptide diaminobutyryl benzylamide diacetate antagonizes the activity of collagenase, etc., prevents the skin around the breast from sagging, and helps maintain the upright appearance of the breasts; arginine / lysine polypeptide can effectively improve the moisture and elasticity of the breast skin, enhance the overall effect of the breast enhancement composition, and multiple peptides synergistically enhance the breast enhancement effect. DETAILED DESCRIPTION

[0016] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0017] The pueraria lobata root extracts in all the embodiments and comparative examples of the present invention were purchased directly from the market, and were purchased from Jiahao Biotechnology (Guangzhou) Co., Ltd.; SINENSIS fruit extracts were purchased directly from the market, all purchased from Hongze (Guangzhou) Biotechnology Co., Ltd.; ginseng root extracts were purchased directly from the market, all purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd.; acetyl hexapeptide-8 was purchased directly from the market, all purchased from Shenzhen Jianyuan Pharmaceutical Technology Co., Ltd.; hexapeptide-9 was purchased directly from the market, all purchased from Shenzhen Jianyuan Pharmaceutical Technology Co., Ltd.; dipeptide diaminobutyryl benzylamide diacetate was purchased directly from the market, and arginine / lysine peptides were purchased from Shenzhen Jianyuan Pharmaceutical Technology Co., Ltd.; Bacillus subtilis was purchased directly from the market, all purchased from Shandong Hui'an Chemical Co., Ltd.; soybean extracts were purchased directly from the market, all purchased from Zhengzhou Yukong Biotechnology Co., Ltd.; Tween-80 was purchased directly from the market, all purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd.; olive oil was purchased directly from supermarkets.

[0018] Example 1 A breast enhancement composition containing natural plant extracts. The raw materials of the breast enhancement composition of this embodiment include, by weight, 0.8 parts of artichoke (CYNARA SCOLYMUS) leaf extract, 19 parts of hops (HUMULUS LUPULUS) extract, 1.5 parts of kudzu (PUERARIA LOBATA) root extract, 0.3 parts of papaya (CHAENOMELES SINENSIS) fruit extract, 18 parts of ginseng (PANAX GINSENG) root extract, 1.4 parts of acetyl hexapeptide-8, 1.2 parts of hexapeptide-9, 1.2 parts of dipeptide diaminobutyryl benzylamide diacetate, 1.3 parts of arginine / lysine polypeptide and 40 parts of deionized water; The preparation method of the artichoke (CYNARA SCOLYMUS) leaf extract and the hop (HUMULUS LUPULUS) extract comprises the following steps: Step S1, washing and drying safflower, adding it into deionized water at a ratio of 1:8 g / ml, adding Bacillus subtilis accounting for 0.1% of the mass of safflower, and simultaneously adding 0.1 mol / L citric acid to adjust the pH to 6.5, fermenting at 30° C. for 3 days, filtering with a 300-mesh filter cloth after the fermentation to obtain safflower fermentation liquid; Step S2, mixing safflower fermentation liquid, soybean extract, Tween-80 and deionized water in a mass ratio of 1:0.5:0.2:5, adding 0.1 mol / L citric acid to adjust the pH to 6.8, and stirring to obtain a fermentation liquid; Step S3, washing and chopping the artichoke leaves, adding them to the fermentation liquid at a ratio of 1:5 g / ml, adding Bacillus subtilis accounting for 0.12% of the mass of the artichoke leaves, fermenting at 32° C. for 3 days to obtain artichoke leaf fermentation products, filtering with a 300-mesh filter cloth to obtain artichoke leaf fermentation clear liquid, mixing the artichoke leaf fermentation clear liquid and olive oil at a volume ratio of 1:1.4, soaking for 24 hours, and freeze-drying to obtain artichoke (CYNARA SCOLYMUS) leaf extract; Step S4, washing and crushing the hops and passing through a 60-mesh sieve, adding the hops to the fermentation liquid at a ratio of 1:6 g / ml, adding Bacillus subtilis accounting for 0.2% of the mass of the hops, fermenting at 35° C. for 3 days to obtain a hop fermentation product, filtering with a 300-mesh filter cloth to obtain a hop fermentation clear liquid, mixing the hop fermentation clear liquid and olive oil at a volume ratio of 1:1.6, soaking for 26 hours, and freeze-drying to obtain a hop (HUMULUS LUPULUS) extract.

[0019] The effective bacterial content of Bacillus subtilis in this example is 1.1×10 7 The freeze-drying operation was to prefreeze at -40°C for 4 h and then heat to 10°C at a rate of 1°C / h under a pressure of 120 μmHg.

[0020] The preparation method of the breast enhancement composition of this embodiment comprises the following steps: Step 1, adding artichoke (CYNARA SCOLYMUS) leaf extract, hops (HUMULUS LUPULUS) extract, kudzu (PUERARIA LOBATA) root extract, papaya (CHAENOMELES SINENSIS) fruit extract, and ginseng (PANAXGINSENG) root extract into deionized water, and mixing by ultrasonication to obtain a mixture; Step 2: Add acetyl hexapeptide-8 and hexapeptide-9 to preheated deionized water, stir at medium speed for 10 minutes, then add dipeptide diaminobutyryl benzylamide diacetate, stir at medium speed for 5 minutes, then add arginine / lysine polypeptide, stir at medium speed for 4 minutes, transfer the obtained mixture to a high shear emulsifier for staged emulsification, adjust the pH at the same time, filter, and obtain 4D anti-wrinkle composite peptide; Step 3: Add 4D anti-wrinkle complex peptide into the mixture in a high-pressure shear emulsifier and start the high-pressure shear emulsifier to process to obtain a breast enhancement composition.

[0021] The parameters of ultrasonic mixing in step 1 of this embodiment are: ultrasonic frequency of 40 kHz, ultrasonic frequency of 100 W, ultrasonic temperature of 38° C., and ultrasonic time of 10 min.

[0022] The mass ratio of the deionized water in step 1 and step 2 of this embodiment is 2:1; the temperature of the preheated deionized water in step 2 is 50°C.

[0023] The speed of medium-speed stirring in step 2 of this embodiment is 300 rpm; the staged emulsification is first stirred at 2000 rpm for 2 minutes, and then stirred at 6000 rpm for 15 minutes; the pH adjustment refers to adjusting the pH to 5.0 using 0.15 mol / L citric acid; and the filtration uses a 0.22 μm filter membrane.

[0024] The rotation speed and time of the high pressure shear emulsifier treatment in step 3 of this embodiment are 3000 rpm and 10 min respectively.

[0025] The amount of the breast enhancement composition of this embodiment added to the breast enhancement product is 7.5wt%.

[0026] Example 2 A breast enhancement composition containing natural plant extracts. The raw materials of the breast enhancement composition of this embodiment include, by weight, 1.2 parts of artichoke (CYNARA SCOLYMUS) leaf extract, 21 parts of hops (HUMULUS LUPULUS) extract, 2.5 parts of kudzu (PUERARIA LOBATA) root extract, 0.7 parts of papaya (CHAENOMELES SINENSIS) fruit extract, 22 parts of ginseng (PANAX GINSENG) root extract, 1.6 parts of acetyl hexapeptide-8, 1.3 parts of hexapeptide-9, 1.4 parts of dipeptide diaminobutyryl benzylamide diacetate, 1.5 parts of arginine / lysine polypeptide and 50 parts of deionized water; The preparation method of the artichoke (CYNARA SCOLYMUS) leaf extract and the hop (HUMULUS LUPULUS) extract comprises the following steps: Step S1, washing and drying safflower, adding it into deionized water at a ratio of 1:12 g / ml, adding Bacillus subtilis accounting for 0.2% of the mass of safflower, and simultaneously adding 0.3 mol / L citric acid to adjust the pH to 7.5, fermenting at 40° C. for 7 days, filtering with a 300-mesh filter cloth after the fermentation to obtain safflower fermentation liquid; Step S2, mixing safflower fermentation liquid, soybean extract, Tween-80 and deionized water in a mass ratio of 1:1.1:0.3:9, adding 0.3 mol / L citric acid dropwise to adjust the pH to 7.2, and stirring to obtain a fermentation liquid; Step S3, washing and chopping the artichoke leaves, adding them to the fermentation liquid at a ratio of 1:7 g / ml, adding Bacillus subtilis accounting for 0.18% of the mass of the artichoke leaves, fermenting at 34° C. for 5 days to obtain artichoke leaf fermentation products, filtering with a 300-mesh filter cloth to obtain artichoke leaf fermentation clear liquid, mixing the artichoke leaf fermentation clear liquid and olive oil at a volume ratio of 1:1.8, soaking for 28 hours, and freeze-drying to obtain artichoke (CYNARA SCOLYMUS) leaf extract; Step S4, washing and crushing the hops, passing through a 100-mesh sieve, adding the hops to the fermentation liquid at a ratio of 1:6.8 g / ml, and adding Bacillus subtilis accounting for 0.22% of the mass of the hops, fermenting at 37° C. for 5 days to obtain a hop fermentation product, filtering with a 300-mesh filter cloth to obtain a hop fermentation clear liquid, mixing the hop fermentation clear liquid and olive oil at a volume ratio of 1:2, soaking for 32 hours, and freeze-drying to obtain a hop (HUMULUS LUPULUS) extract.

[0027] The effective bacterial content of Bacillus subtilis in this example is 1.3×10 7 CFU / g; freeze-drying operation was to pre-freeze at -40℃ for 6h, then heat to 10℃ at a rate of 2℃ / h under a pressure of 160μmHg.

[0028] The preparation method of the breast enhancement composition of this embodiment comprises the following steps: Step 1, adding artichoke (CYNARA SCOLYMUS) leaf extract, hops (HUMULUS LUPULUS) extract, kudzu (PUERARIA LOBATA) root extract, papaya (CHAENOMELES SINENSIS) fruit extract, and ginseng (PANAXGINSENG) root extract into deionized water, and mixing by ultrasonication to obtain a mixture; Step 2: Add acetyl hexapeptide-8 and hexapeptide-9 to preheated deionized water, stir at medium speed for 20 minutes, then add dipeptide diaminobutyryl benzylamide diacetate, stir at medium speed for 10 minutes, then add arginine / lysine polypeptide, stir at medium speed for 10 minutes, transfer the obtained mixture to a high shear emulsifier for staged emulsification, adjust the pH at the same time, filter, and obtain 4D anti-wrinkle composite peptide; Step 3: Add 4D anti-wrinkle complex peptide into the mixture in a high-pressure shear emulsifier and start the high-pressure shear emulsifier to process to obtain a breast enhancement composition.

[0029] The parameters of ultrasonic mixing in step 1 of this embodiment are: ultrasonic frequency of 60 kHz, ultrasonic frequency of 500 W, ultrasonic temperature of 46° C., and ultrasonic time of 20 min.

[0030] The mass ratio of the deionized water in step 1 and step 2 of this embodiment is 3:1; the temperature of the preheated deionized water in step 2 is 60°C.

[0031] The speed of medium-speed stirring in step 2 of this embodiment is 400 rpm; the staged emulsification is first stirred at 4000 rpm for 8 minutes, and then stirred at 10000 rpm for 25 minutes; the pH adjustment refers to adjusting the pH to 6.5 using 0.25 mol / L citric acid; and the filtration uses a 0.22 μm filter membrane.

[0032] The rotation speed and time of the high pressure shear emulsifier treatment in step 3 of this embodiment are 6000 rpm and 20 min respectively.

[0033] The amount of the breast enhancement composition of this embodiment added to the breast enhancement product is 8.5wt%.

[0034] Example 3 A breast enhancement composition containing natural plant extracts. The raw materials of the breast enhancement composition of this embodiment include, by weight, 1 part of artichoke (CYNARA SCOLYMUS) leaf extract, 20 parts of hops (HUMULUS LUPULUS) extract, 2 parts of kudzu (PUERARIA LOBATA) root extract, 0.5 parts of papaya (CHAENOMELES SINENSIS) fruit extract, 20 parts of ginseng (PANAX GINSENG) root extract, 1.5 parts of acetyl hexapeptide-8, 1.25 parts of hexapeptide-9, 1.3 parts of dipeptide diaminobutyryl benzylamide diacetate, 1.4 parts of arginine / lysine polypeptide and 45 parts of deionized water; The preparation method of the artichoke (CYNARA SCOLYMUS) leaf extract and the hop (HUMULUS LUPULUS) extract comprises the following steps: Step S1, washing and drying safflower, adding it into deionized water at a ratio of 1:10 g / ml, adding Bacillus subtilis accounting for 0.15% of the mass of safflower, and simultaneously adding 0.2 mol / L citric acid to adjust the pH to 7, fermenting at 35° C. for 5 days, filtering with a 300-mesh filter cloth after the fermentation is completed, and obtaining safflower fermentation liquid; Step S2, mixing safflower fermentation liquid, soybean extract, Tween-80 and deionized water in a mass ratio of 1:0.8:0.25:7, adding 0.2 mol / L citric acid dropwise to adjust the pH to 7, and stirring to obtain a fermentation liquid; Step S3, washing and chopping the artichoke leaves, adding them to the fermentation liquid at a ratio of 1:6 g / ml, adding Bacillus subtilis accounting for 0.15% of the mass of the artichoke leaves, fermenting at 33° C. for 4 days to obtain artichoke leaf fermentation products, filtering with a 300-mesh filter cloth to obtain artichoke leaf fermentation clear liquid, mixing the artichoke leaf fermentation clear liquid and olive oil at a volume ratio of 1:1.6, soaking for 26 hours, and freeze-drying to obtain artichoke (CYNARA SCOLYMUS) leaf extract; Step S4, washing and crushing the hops and passing through an 80-mesh sieve, adding the hops to the fermentation liquid at a ratio of 1:6.4 g / ml, adding Bacillus subtilis accounting for 0.21% of the mass of the hops, fermenting at 36° C. for 4 days to obtain a hop fermentation product, filtering with a 300-mesh filter cloth to obtain a hop fermentation clear liquid, mixing the hop fermentation clear liquid and olive oil at a volume ratio of 1:1.8, soaking for 29 hours, and freeze-drying to obtain a hop (HUMULUS LUPULUS) extract.

[0035] The effective bacterial content of Bacillus subtilis in this example is 1.2×10 7 The freeze-drying operation was to prefreeze at -40°C for 5 h and then heat to 10°C at a rate of 1.5°C / h under a pressure of 140 μmHg.

[0036] The preparation method of the breast enhancement composition of this embodiment comprises the following steps: Step 1, adding artichoke (CYNARA SCOLYMUS) leaf extract, hops (HUMULUS LUPULUS) extract, kudzu (PUERARIA LOBATA) root extract, papaya (CHAENOMELES SINENSIS) fruit extract, and ginseng (PANAXGINSENG) root extract into deionized water, and mixing by ultrasonication to obtain a mixture; Step 2: Add acetyl hexapeptide-8 and hexapeptide-9 to preheated deionized water, stir at medium speed for 15 minutes, then add dipeptide diaminobutyryl benzylamide diacetate, stir at medium speed for 7.5 minutes, then add arginine / lysine polypeptide, stir at medium speed for 7 minutes, transfer the obtained mixture to a high shear emulsifier for staged emulsification, adjust the pH at the same time, filter, and obtain 4D anti-wrinkle composite peptide; Step 3: Add 4D anti-wrinkle complex peptide into the mixture in a high-pressure shear emulsifier and start the high-pressure shear emulsifier to process to obtain a breast enhancement composition.

[0037] The parameters of ultrasonic mixing in step 1 of this embodiment are: ultrasonic frequency of 50 kHz, ultrasonic frequency of 300 W, ultrasonic temperature of 42° C., and ultrasonic time of 15 min.

[0038] The mass ratio of deionized water in step 1 and step 2 of this embodiment is 2.5:1; the temperature of the preheated deionized water in step 2 is 55°C.

[0039] The speed of medium-speed stirring in step 2 of this embodiment is 350 rpm; the staged emulsification is first stirred at 3000 rpm for 5 minutes, and then stirred at 8000 rpm for 20 minutes; the pH adjustment refers to adjusting the pH to 5.8 using 0.2 mol / L citric acid; and the filtration uses a 0.22 μm filter membrane.

[0040] The rotation speed and time of the high pressure shear emulsifier treatment in step 3 of this embodiment are 4500 rpm and 15 min respectively.

[0041] The amount of the breast enhancement composition of this embodiment added to the breast enhancement product is 8 wt %.

[0042] Comparative Example 1 On the basis of Example 3, the artichoke (CYNARA SCOLYMUS) leaf extract in the breast enhancement composition was removed and replaced with an equal weight of hop (HUMULUS LUPULUS) extract, and other conditions were the same as those in Example 3.

[0043] Comparative Example 2 On the basis of Example 3, the hops (HUMULUS LUPULUS) extract in the breast enhancement composition was removed and replaced with an equal weight of pueraria lobata (PUERARIA LOBATA) root extract. Other conditions were the same as those in Example 3.

[0044] Comparative Example 3 On the basis of Example 3, the Pueraria lobata root extract in the breast enhancement composition was removed and replaced with an equal weight of CHAENOMELES SINENSIS fruit extract. Other conditions were the same as those in Example 3.

[0045] Comparative Example 4 On the basis of Example 3, the papaya (CHAENOMELES SINENSIS) fruit extract in the breast enhancement composition was removed and replaced with an equal weight of ginseng (PANAX GINSENG) root extract. Other conditions were the same as those in Example 3.

[0046] Comparative Example 5 On the basis of Example 3, the ginseng root extract in the breast enhancement composition was removed and replaced with an equal weight of 4D anti-wrinkle complex peptide, and other conditions were consistent with Example 3.

[0047] Comparative Example 6 On the basis of Example 3, the 4D anti-wrinkle complex peptide in the breast enhancement composition was removed and replaced with an equal weight of CYNARA SCOLYMUS leaf extract, and other conditions were the same as those in Example 3.

[0048] Comparative Example 7 On the basis of Example 3, the safflower fermented liquid in the fermented liquid was removed and replaced with an equal weight of soybean extract, and the other conditions were the same as those in Example 3.

[0049] Comparative Example 8 On the basis of Example 3, the soybean extract in the fermentation broth was removed and replaced with an equal weight of safflower fermentation broth. Other conditions were the same as those in Example 3.

[0050] Comparative Example 9 On the basis of Example 3, acetyl hexapeptide-8 in the 4D anti-wrinkle composite peptide was removed and replaced with an equal weight of hexapeptide-9, and other conditions were consistent with Example 3.

[0051] Comparative Example 10 On the basis of Example 3, hexapeptide-9 in the 4D anti-wrinkle composite peptide was removed and replaced with an equal weight of dipeptide diaminobutyrylbenzylamide diacetate, and other conditions were consistent with Example 3.

[0052] Comparative Example 11 On the basis of Example 3, the dipeptide diaminobutyrylbenzylamide diacetate in the 4D anti-wrinkle composite peptide was removed and replaced with an equal weight of arginine / lysine polypeptide, and other conditions were consistent with Example 3.

[0053] Comparative Example 12 On the basis of Example 3, the arginine / lysine polypeptide in the 4D anti-wrinkle composite peptide was removed and replaced with an equal weight of acetyl hexapeptide-8, and the other conditions were consistent with Example 3.

[0054] Comparative Example 13 On the basis of Example 3, keeping other conditions the same, the steps of the preparation method of the artichoke (CYNARA SCOLYMUS) leaf extract and the hop (HUMULUS LUPULUS) extract were changed to: Step S1, washing and drying safflower, adding it into deionized water at a ratio of 1:10 g / ml, adding Bacillus subtilis accounting for 0.15% of the mass of safflower, and simultaneously adding 0.2 mol / L citric acid to adjust the pH to 7, fermenting at 35° C. for 5 days, filtering with a 300-mesh filter cloth after the fermentation is completed, and obtaining safflower fermentation liquid; Step S2, mixing safflower fermentation liquid, soybean extract, Tween-80 and deionized water in a mass ratio of 1:0.8:0.25:7, adding 0.2 mol / L citric acid dropwise to adjust the pH to 7, and stirring to obtain a fermentation liquid; Step S3, washing and chopping the artichoke leaves, adding them to the fermentation liquid at a ratio of 1:6 g / ml, and adding 0.15% of the mass of the artichoke leaves of Bacillus subtilis, fermenting at 33° C. for 4 days to obtain artichoke leaf fermentation products, filtering the artichoke leaf fermentation clear liquid with a 300-mesh filter cloth, and freeze-drying to obtain artichoke (CYNARA SCOLYMUS) leaf extract; Step S4, washing and crushing the hops, passing through an 80-mesh sieve, adding the hops to the fermentation liquid at a ratio of 1:6.4 g / ml, and adding Bacillus subtilis accounting for 0.21% of the mass of the hops, fermenting at 36° C. for 4 days to obtain a hop fermentation product, filtering the hop fermentation clear liquid with a 300-mesh filter cloth, and freeze-drying to obtain a hop (HUMULUS LUPULUS) extract.

[0055] 1. Patch test Fifteen volunteers with healthy skin were selected, half of them were male and half were female, and their ages were between 20 and 45. All volunteers had no history of skin disease, no known allergy to the test materials, and had not used any drugs or cosmetics that might affect skin reactions within at least 2 weeks before the test.

[0056] The breast enlargement composition obtained by extraction in Examples 1-3 was used as a sample, and the samples were dissolved in deionized water to prepare a sample solution with a concentration of 8%. 0.02±0.005 ml of the sample solution was dripped into a spot tester, and attached to the flexor side of the forearm of the subject with a low-allergenic tape. After 24 hours, the spot tester was removed, and the skin reaction was observed 0.5 hours, 24 hours, and 48 hours after removal. The results were recorded according to the skin reaction grading standard in the "Safety Technical Specifications for Cosmetics" (2015 edition), where the scoring grading is divided into 0-4 levels, and 0 is a negative reaction. The test results are shown in Table 1.

[0057] Table 1 Patch test results

[0058] As can be seen from Table 1, the breast enhancement compositions containing natural plant extracts prepared in Examples 1-3 of the present invention are mild to the skin and have no adverse reactions when used.

[0059] 2. The breast enlargement composition obtained by extracting Example 3 and Comparative Examples 1-13 was used as a sample, and the sample was dissolved in deionized water to prepare a sample solution with a concentration of 8%. 140 female volunteers aged 20-50 were randomly divided into 14 groups, with 10 people in each group. All volunteers had no history of skin disease, no known allergy to the test material, and had not used any drugs or cosmetics that may affect skin reaction within at least 2 weeks before the test. The method of use was to apply the test solution to the chest surface in the morning and evening, gently massage until absorbed, and use it continuously for 3 months without using other products during the period. After the test, the effect of use was evaluated, wherein the chest was significantly enlarged and the contour was fuller and more upright as marked effect, the chest was enlarged and the sagging and outward expansion phenomenon was improved as effective, and the chest state did not show significant changes as invalid, and the overall effective rate was calculated and recorded as shown in Table 2 below; at the same time, the change in chest elasticity was scored according to the 10-point system according to the evaluation of the volunteers, and the average score was recorded as shown in Table 2 below.

[0060] Table 2 Breast enhancement effectiveness and elasticity evaluation

[0061] It can be seen from Table 2 that the breast enhancement composition containing natural plant extracts prepared in the embodiment of the present invention can effectively improve skin elasticity, plump up breasts, and exert breast enhancement effects.

[0062] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any indirect modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A breast enhancement composition containing natural plant extracts, characterized in that: The raw materials of the breast enlargement composition include, by weight, 0.8-1.2 parts of cynarin (CYNARA SCOLYMUS) leaf extract, 19-21 parts of hop (HUMULUSLUPULUS) extract, 1.5-2.5 parts of pueraria (PUERARIA LOBATA) root extract, 0.3-0.7 parts of papaya (CHAENOMELES SINENSIS) fruit extract, 18-22 parts of ginseng (PANAX GINSENG) root extract, 1.4-1.6 parts of acetyl hexapeptide-8, 1.2-1.3 parts of hexapeptide-9, 1.2-1.4 parts of dipeptide diaminobutyryl benzylamide diacetate, 1.3-1.5 parts of arginine / lysine polypeptide and 40-50 parts of deionized water; The preparation method of the artichoke (CYNARA SCOLYMUS) leaf extract and the hop (HUMULUS LUPULUS) extract comprises the following steps: Step S1, washing and drying safflower, adding it into deionized water at a ratio of 1:8-12 g / ml, adding Bacillus subtilis accounting for 0.1-0.2% of the mass of safflower, and simultaneously adding 0.1-0.3 mol / L citric acid dropwise to adjust the pH to 6.5-7.5, fermenting at 30-40° C. for 3-7 days, filtering with a 300-mesh filter cloth after the fermentation is completed, and obtaining safflower fermentation liquid; Step S2, mixing safflower fermentation liquid, soybean extract, Tween-80 and deionized water in a mass ratio of 1:0.5-1.1:0.2-0.3:5-9, and adding 0.1-0.3 mol / L citric acid to adjust the pH to 6.8-7.2, and stirring to obtain fermentation liquid; Step S3, washing and chopping the artichoke leaves, adding them to the fermentation liquid at a ratio of 1:5-7 g / ml, adding Bacillus subtilis accounting for 0.12-0.18% of the mass of the artichoke leaves, fermenting at 32-34° C. for 3-5 days to obtain artichoke leaf fermentation products, filtering with a 300-mesh filter cloth to obtain artichoke leaf fermentation clear liquid, mixing the artichoke leaf fermentation clear liquid and olive oil at a volume ratio of 1:1.4-1.8, soaking for 24-28 hours, and freeze-drying to obtain artichoke (CYNARA SCOLYMUS) leaf extract; Step S4, washing and crushing the hops, passing through a 60-100 mesh sieve, adding the hops to the fermentation liquid at a ratio of 1:6-6.8 g / ml, adding Bacillus subtilis accounting for 0.2-0.22% of the mass of the hops, fermenting at 35-37° C. for 3-5 days to obtain a hop fermentation product, filtering with a 300-mesh filter cloth to obtain a hop fermentation clear liquid, mixing the hop fermentation clear liquid and olive oil at a volume ratio of 1:1.6-2, soaking for 26-32 hours, and freeze-drying to obtain a hop (HUMULUS LUPULUS) extract.

2. A breast enhancement composition containing natural plant extracts according to claim 1, characterized in that: The effective bacterial content of Bacillus subtilis is 1.1-1.3×10 7 CFU / g; the freeze-drying operation is to pre-freeze at -40°C for 4-6 hours, and then heat to 10°C at a heating rate of 1-2°C / h under a pressure of 120-160μmHg.

3. A method for preparing a breast enhancement composition containing natural plant extracts as claimed in any one of claims 1 to 2, characterized in that: The preparation method of the breast enhancement composition comprises the following steps: Step 1, adding artichoke (CYNARA SCOLYMUS) leaf extract, hops (HUMULUS LUPULUS) extract, kudzu (PUERARIA LOBATA) root extract, papaya (CHAENOMELES SINENSIS) fruit extract, and ginseng (PANAXGINSENG) root extract into deionized water, and mixing by ultrasonication to obtain a mixture; Step 2: Add acetyl hexapeptide-8 and hexapeptide-9 to preheated deionized water, stir at medium speed for 10-20 minutes, then add dipeptide diaminobutyryl benzylamide diacetate, stir at medium speed for 5-10 minutes, then add arginine / lysine polypeptide, stir at medium speed for 4-10 minutes, transfer the obtained mixed solution to a high shear emulsifier for staged emulsification, adjust the pH at the same time, filter, and obtain 4D anti-wrinkle composite peptide; Step 3: Add 4D anti-wrinkle complex peptide into the mixture in a high-pressure shear emulsifier and start the high-pressure shear emulsifier to process to obtain a breast enhancement composition.

4. The method for preparing a breast enhancement composition containing natural plant extracts according to claim 3, characterized in that: The parameters of the ultrasonic mixing in step 1 are: ultrasonic frequency of 40-60kHz, ultrasonic frequency of 100-500W, ultrasonic temperature of 38-46°C, and ultrasonic time of 10-20min.

5. The method for preparing a breast enhancement composition containing natural plant extracts according to claim 3, characterized in that: The mass ratio of the deionized water in step 1 and step 2 is 2-3:1; the temperature of the preheated deionized water in step 2 is 50-60°C.

6. The method for preparing a breast enhancement composition containing natural plant extracts according to claim 3, characterized in that: The speed of the medium-speed stirring in step 2 is 300-400rpm; the segmented emulsification is first stirred at a speed of 2000-4000rpm for 2-8min, and then stirred at a speed of 6000-10000rpm for 15-25min; the pH adjustment refers to adjusting the pH to 5.0-6.5 using 0.15-0.25mol / L citric acid; and the filtration uses a 0.22μm filter membrane.

7. The method for preparing a breast enhancement composition containing natural plant extracts according to claim 3, characterized in that: The rotation speed and time of the high-pressure shear emulsifier treatment in step 3 are 3000-6000 rpm and 10-20 min respectively.

8. A method for preparing a breast enhancement composition containing natural plant extracts according to any one of claims 3 to 7, characterized in that: The breast enhancement composition is added in an amount of 7.5-8.5 wt % in breast enhancement products.

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