Polypeptide with cell adhesion effect and application thereof

Abstract

The invention discloses a polypeptide with a cell adhesion effect and application thereof. The general formula of the polypeptide sequence is as follows: R1-(G-G-G) n-RGDY-OH, wherein R1 is sodium hyaluronate, G is glycine, R is arginine, D is aspartic acid, and Y is tyrosine. When n is equal to 2, the polypeptide is dodecapeptide, has very strong cell adhesiveness and also has skin barrier and moisturizing effects, and the cell adhesiveness can further enhance the skin barrier and moisturizing effects, so that a synergistic effect is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and specifically relates to a polypeptide with cell adhesion function and application thereof. Background Art

[0002] When the barrier function of the skin is damaged, the moisture in the epidermis will be lost through the stratum corneum, making the skin dry, thus causing or aggravating some skin problems. The skin needs a stable internal environment to maintain normal physiological functions. Moisturizing cosmetics are the most commonly used skin care products by simulating the skin's natural moisturizing system to maintain the skin's moisture content. Common moisturizing cosmetics are mostly composed of oils and hygroscopic ingredients. Oils are responsible for forming an oil film to prevent water evaporation, and hygroscopic ingredients can absorb moisture from the environment and increase the water content of the skin's stratum corneum. However, these only look moist on the surface and cannot achieve deep moisturizing.

[0003] In recent years, peptides with cell adhesion properties have been discovered, such as RGD peptide. RGD peptide can be specifically recognized by integrin αvβ3, thus having cell adhesion effect. However, the adhesion effect is not very strong in actual applications. Our team previously coupled RGD peptide with hyaluronic acid and found that it can achieve the effect of adhering cells to enhance the moisturizing effect of hyaluronic acid, but the improvement effect is not very obvious. Subsequently, we will further improve the polypeptide chain in an effort to further enhance the cell adhesion and moisturizing and repair effects. Summary of the invention

[0004] The purpose of the present invention is to provide a polypeptide having cell adhesion and moisturizing and repairing effects.

[0005] In order to achieve the above object, the technical solution adopted by the present invention includes:

[0006] In a first aspect, the present invention provides a polypeptide having cell adhesion function, wherein the general formula of the polypeptide sequence is: R1-(GGGG) n -RGDY-OH;

[0007] Among them, R1 is sodium hyaluronate, G is glycine, R is arginine, D is aspartic acid, and Y is tyrosine.

[0008] Furthermore, when n is 1 in the general formula, the polypeptide sequence is R1-GGGG-RGDY-OH.

[0009] Furthermore, when n is 2 in the general formula, the polypeptide sequence is R1-GGGGGGGG-RGDY-OH.

[0010] Furthermore, when n is 3 in the general formula, the polypeptide sequence is R1-GGGGGGGGGGGG-RGDY-OH

[0011] In a second aspect, the present invention provides a use of a polypeptide with cell adhesion function in the preparation of moisturizing and repairing cosmetics.

[0012] Furthermore, the moisturizing and repairing is achieved by promoting the expression of skin barrier-related genes LOR, K1, AQP3, CLDN-4 and / or FLG.

[0013] Furthermore, the cosmetic is one of essence, toner, lotion or cream.

[0014] The beneficial effects of the present invention are:

[0015] The polypeptide with cell adhesion prepared by the present invention has the dual effects of cell adhesion and moisturizing, and the gene expression levels of five barrier moisturizing-related aspects, namely LOR, K1, AQP3, CLDN-4 and FLG, are significantly increased.

[0016] The polypeptide with cell adhesion prepared by the present invention can have different lengths of peptide chains when n in the general formula is different, and the expression of cell adhesion and barrier moisturizing genes is different, among which n=2 is the best. When n=2, the polypeptide is a dodecapeptide, which has strong cell adhesion, and the effect of barrier moisturizing gene regulation is also enhanced. Cell adhesion means that the polypeptide can better adhere to cells, which will further enhance the skin barrier and moisturizing effects, achieving a synergistic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention is further described below in conjunction with the accompanying drawings.

[0018] Figure 1 H-(GGGG) prepared in Example 1 of the present invention 2 The mass spectrum of -RGDY-OH shows M+1=966, and its molecular weight is 965.

[0019] Figure 2 H-(GGGG) prepared in Example 1 of the present invention 2 The liquid chromatogram of -RGDY-OH, HPLC showed that 7.412 minutes was the peak of its liquid phase.

[0020] Figure 3 This is a comparison chart of the expression levels of AQP3, FLG, LOR, CLdn-4, and K1 related genes in the efficacy verification of the present invention.

[0021] Figure 4 This is a real picture of the cell adhesion test results in the efficacy verification of the present invention.

[0022] Figure 5 This is a statistical analysis chart of the number of cells in the cell adhesion test results in the efficacy verification of the present invention. DETAILED DESCRIPTION

[0023] In order to better understand the present invention, the present invention is explained in detail below through the description of preferred specific embodiments of the present invention, but the present invention is not limited in any form.

[0024] Unless otherwise specified, the raw materials or chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels.

[0025] Example 1

[0026] This example provides a method for preparing a polypeptide having cell adhesion function.

[0027] R1-(GGGG) n -RGDY-OH, when n=2, is prepared according to the following steps:

[0028] 1. H-(GGGG) 2 Solid Phase Synthesis of -RGDY-OH Resin Peptide

[0029] 2-Immersion of CTC Resin

[0030] Take 10g of 2-CTC Resin (substitution degree 1.57mmol / g), put it into a glass reactor, add 100ml of DCM, soak and stir for 30min, and protect with nitrogen;

[0031] 1) Ligation of Fmoc-Tyr(otbu)-OH

[0032] Take Fmoc-Tyr(otbu)-OH (1.5eq), DCM 100ml, DIEA (6eq), stir to dissolve, and add to a glass reactor; react for 2 hours, add 10ml anhydrous methanol, stir for 30 minutes, filter out DCM, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, and filter out DMF;

[0033] Deprotection of Fmoc

[0034] Add 100ml 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 100ml IPA for 5 minutes, then wash twice with 200ml DMF, each time for 5 minutes, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0035] 2) Ligation of Fmoc-Asp(otbu)-OH

[0036] Take Fmoc-Asp(otbu)-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF100ml, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF;

[0037] Deprotection of Fmoc

[0038] Add 100ml 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 1L IPA (5 minutes), then wash twice with 2L DMF, 5 minutes each time, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0039] 3) Fmoc-Gly-OH ligation

[0040] Take Fmoc-Gly-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF 100ml, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF;

[0041] Deprotection of Fmoc

[0042] Add 100 ml of 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 1 L of IPA for 5 minutes, then wash twice with 200 ml DMF, each time for 5 minutes, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0043] 4) Ligation of Fmoc-Arg(pbf)-OH

[0044] Take Fmoc-Arg(pbf)-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF100ml, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF;

[0045] Deprotection of Fmoc

[0046] Add 100ml 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 1L IPA for 5 minutes, then wash twice with 200ml 2L DMF, each time for 5 minutes, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0047] 5) Ligation of Fmoc-Gly-Gly-OH

[0048] Take Fmoc-Gly-Gly-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF2L, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF;

[0049] Deprotection of Fmoc

[0050] Add 100ml 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 100ml IPA (5 minutes), then wash twice with 200ml DMF, 5 minutes each time, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0051] 6) Ligation of Fmoc-Gly-Gly-OH

[0052] Take Fmoc-Gly-Gly-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF100ml, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF;

[0053] Deprotection of Fmoc

[0054] Add 100ml 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 1L IPA (5 minutes), then wash twice with 200ml DMF, 5 minutes each time, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0055] 7) Ligation of Fmoc-Gly-Gly-OH

[0056] Take Fmoc-Gly-Gly-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF 100ml, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF;

[0057] Deprotection of Fmoc

[0058] Add 100ml 20% Pip / DMF, stir for half an hour, filter out the solvent, wash once with 100ml IPA for 5 minutes, then wash twice with 200ml DMF, each time for 5 minutes, finally filter out the solvent, take a small amount of resin and add 5% ninhydrin for detection, which shows positive (black).

[0059] 8) Boc-Gly-Gly-OH ligation

[0060] Take Boc-Gly-Gly-OH (1.5eq), HBTU (1.5eq), DIEA (2eq), DMF100ml, stir and dissolve, and add to a glass reactor; react for 2 hours, take a small amount of resin and add 5% ninhydrin to detect, showing negative (colorless), filter out DMF, add 100ml IPA to wash once, wash for 5 minutes, filter out IPA, add 100ml DMF to wash once, wash for 5 minutes, filter out DMF; add 200ml methanol to wash twice, 5 minutes each time, drain, take out, and dry at 50°;

[0061] 2. H-(GGGG) 2 Crude preparation of -RGDY-OH

[0062] 1) Preparation of cutting fluid

[0063] CF3COOH: TIS: water = 95:4:1

[0064] 2) Cleavage of resin peptide

[0065] Put the resin peptide into the reactor, slowly add the cutting liquid, add a stirrer, stir magnetically for 2 hours, filter, take the reaction solution, vacuum concentrate the reaction solution at 40° until it becomes viscous, add 100ml of tert-butyl ether, stir rapidly, precipitate a white solid, stir for half an hour, centrifuge, pour off the supernatant, wash with 100ml of ethyl acetate, centrifuge, wash with 100ml of petroleum ether, centrifuge; finally take out the white solid, dry it with air at 40°; get white H-(GGGG) 2 -RGDY-OH crude product 12g;

[0066] 3. H-(GGGG) 2 Preparation of pure -RGDY-OH

[0067] 1) Dissolution of crude product: weigh up to 12.0g of crude product (maximum sample load of DAC-100 dynamic axial compression column is 12.0g) in a beaker, crush with a spoon, dissolve in 95% water + 5% acetonitrile to 20mg / ml, use an ultrasonic device to accelerate the dissolution, filter with a 0.45um organic filter membrane after dissolution, and wait for loading

[0068] 2) Equilibration: Run the semi-preparative liquid phase for more than 15 minutes according to the following method until the baseline is stable

[0069] 3) Sampling: Take the filtered sample and load it with 100% A pump. When the sample is almost finished, rinse the filter head with 20ml purified water to dilute it. Repeat three times. After the eluent injection is completed, stop all pumps.

[0070] 4) Salt transfer: Run the semi-preparative liquid phase for more than 10 minutes according to the following method

[0071] 5) Gradient elution: After loading, run the equipment as follows

[0072]

[0073] During the gradient run, the signal absorption peaks are collected and numbered, and the chromatographic peak information of each fragment number is recorded. It is recommended that the volume of the collected liquid in each tube should not exceed 250ml. After the run is completed, the collected samples are analyzed and tested.

[0074] 6) Analysis and testing: The collected samples are analyzed and tested, and the samples with purity greater than 90% are recorded as Final samples, and then freeze-dried after concentration.

[0075] 7) Freeze-drying: Collect all samples with purity greater than 90%, mix them for analysis and testing, concentrate them and freeze-dry them to obtain H-(GGGG) 2-RGDY-OH pure acetate 5g;

[0076] H-(GGGG) 2 -RGDY-OH is coupled with sodium hyaluronate to obtain HA-(GGGG) 2 -RGDY-OH, named R12-HA, is a polypeptide with cell adhesion effect.

[0077] Example 2

[0078] This example provides a method for preparing a polypeptide having cell adhesion function.

[0079] R1-(GGGG) n -RGDY-OH, when n=1, the number of connections to Fmoc-Gly-Gly-OH is different, and the rest is the same as in Example 1, and the following is an abbreviated step:

[0080] Using Fmoc-protected amino acids as raw materials, 2-CTC Resin (substitution degree 1.0 mmol / g) as a solid phase carrier, and DIEA / HBTU as a condensing agent, the octapeptide compound was synthesized, and finally cleaved from the resin and purified by high performance liquid chromatography;

[0081] All samples with a purity greater than 90% were collected, mixed for analysis and testing, concentrated and freeze-dried to obtain 5 g of pure acetate of H-(GGGG)-RGDY-OH;

[0082] H-(GGGG)-RGDY-OH was coupled with sodium hyaluronate to obtain HA-(GGGG)-RGDY-OH, named R8-HA, which is a polypeptide with cell adhesion effect.

[0083] Example 3

[0084] This example provides a method for preparing a polypeptide having cell adhesion function.

[0085] R1-(GGGG) n -RGDY-OH, when n=3, the number of connections to Fmoc-Gly-Gly-OH is different, and the rest is the same as in Example 1, and the following is an abbreviated step:

[0086] Using Fmoc-protected amino acids as raw materials, 2-CTC Resin (substitution degree 1.0 mmol / g) as a solid phase carrier, and DIEA / HBTU as a condensing agent, a hexadecapeptide compound was synthesized, which was finally cleaved from the resin and purified by HPLC.

[0087] Collect all samples with purity greater than 90%, mix them for analysis and testing, concentrate them and freeze-dry them to obtain H-(GGGG) 3 -RGDY-OH pure acetate 5g;

[0088] H-(GGGG) 3 -RGDY-OH is coupled with sodium hyaluronate to obtain HA-(GGGG) 3 -RGDY-OH, named R16-HA, thus obtaining a polypeptide with cell adhesion function.

[0089] Example 4

[0090] This embodiment provides a method for preparing a polypeptide.

[0091] R1-(GGGG)n-RGDY-OH, when n=4, the number of connections to Fmoc-Gly-Gly-OH is different, and the rest is the same as in Example 1, and the following is an abbreviated step:

[0092] Using Fmoc-protected amino acids as raw materials, 2-CTC Resin (substitution degree 1.0 mmol / g) as a solid phase carrier, and DIEA / HBTU as condensing agents, twenty peptide compounds were synthesized, and finally cleaved from the resin and purified by high performance liquid chromatography;

[0093] Collect all samples with purity greater than 90%, mix them for analysis and testing, concentrate them and freeze-dry them to obtain H-(GGGG) 4 -RGDY-OH pure acetate 5g;

[0094] H-(GGGG) 4 -RGDY-OH was coupled with sodium hyaluronate and named R20-HA, which is a polypeptide.

[0095] Efficacy verification

[0096] 1. Gene level detection:

[0097] Cells: Human immortalized epidermal cells: HaCat cells.

[0098] Reagents or materials: high-glucose DMEM (Gibco), PBS powder (XINTAI), Trypsin (Gibco), disodium ethylenediaminetetraacetic acid / EDTA (Sinopharm), fetal bovine serum / FBS (Sijiqing), TryPsin (Gibco), RNA iS OPLUS (TaKaRa), chloroform (Sinopharm), isopropanol (Sinopharm), 75% alcohol, DEPC water, double distilled water, 6-well plate.

[0099] RNAiso Plus (TaKaRa), Evo M-MLV reverse transcription reagent premix (Acrobio), SYBR GreenPro Taq HS premix qPCR kit (Acrobio).

[0100] Instruments and equipment: electronic balance (Pioneer), spiral mixer (Hangzhou Miou), pipette (Eppendorf), carbon dioxide incubator (Thermo), cell counter (Invitrogen), inverted fluorescence microscope (Nikon), oscillator (Kylin-Bell), clean bench (Sujing Antai), PCR instrument (Roche, Lightcycler96), ultra-micro spectrophotometer (DENOVIX), low-temperature centrifuge (Eppendorf), water bath (Kewei).

[0101] Detection method:

[0102] Sample preparation: Samples (HA, R8-HA, R12-HA) were prepared into a stock solution of 10 mg / mL and diluted according to the required concentration.

[0103] Gene level detection: Real-time fluorescence quantitative PCR method was used to inoculate HaCat cells in the logarithmic growth phase into 6-well plates at a density of 4×105 cells / well in an incubator (37°C, 5% CO 2 ) and incubate overnight. When the cell plating rate in the 6-well plate reaches about 50%, group drug administration is performed, and the concentration is (0.625mg / mL, 0.313mg / mL) and the dosage per well is 2mL. After drug administration, the 6-well plate is placed in an incubator (37℃, 5% CO 2 ) and incubated for 24 h. After the culture, the cells were washed three times with 2 mL / well PBS, and 1 mL RNAiso Plus was added to each well. After blowing and lysing the cells, the cell lysate was collected for RNA extraction, reverse transcription, and fluorescence quantitative PCR detection of gene levels. -△△Ct Method to calculate the results.

[0104] Data statistics: T-Test was performed on the values ​​of the sample group and the SC group using software. P < 0.05 was considered to be significantly different from the SC group, indicated by "*", and P < 0.01 was considered to be extremely significantly different from the SC group, indicated by "**".

[0105] Test results:

[0106] Gene level detection: The identification standard for differentially expressed genes was |log2 Fold Change|>0.5, that is, the fold difference was greater than 1.414 or less than 0.707, and P<0.05. If the above criteria were met at the same time, it was considered to be different.

[0107] The samples were set at two concentrations (0.625 mg / mL and 0.313 mg / mL), and the barrier-moisturizing related genes were detected by fluorescence quantitative PCR. The results are shown in Table 1. Figure 3 .

[0108] Table 1 Results of fluorescent quantitative PCR gene detection

[0109]

[0110] Table 2 Brief annotation of gene function

[0111]

[0112] in conclusion:

[0113] As shown in Table 1, Figure 3 The results showed that samples HA, R8-HA, and R12-HA were acted on HaCat cells for 24 hours. Among them, the levels of AQP3, FLG, LOR, K1, and CLDN-4 genes were significantly increased when R12-HA was exposed to concentrations of 0.625 mg / mL and 0.313 mg / mL. Combined with the brief annotation table of gene functions in Table 2, the overall analysis showed that R12-HA had the best barrier moisturizing effect.

[0114] 2. Cell Adhesion Detection

[0115] (1) Purpose of the experiment

[0116] This experiment was based on human skin fibroblast (HFB) cells to conduct a cell adhesion experiment to determine the interaction between the samples in Examples 1 to 4 and the cells and to determine whether the samples can promote cell adhesion.

[0117] (2) Experimental Materials

[0118] Experimental cells: The cells used in this experiment are human skin fibroblasts (HFB) cells, which were obtained by Guangdong Boxi Biotechnology Co., Ltd. through primary and subculture of cells. The cell generation used in the experiment is P4.

[0119] Experimental reagents: DMEM (Gibco), fetal bovine serum (Sijiqing), PBS (Boster), Hoechst33342 staining solution (Biyuntian), and trypsin (Gibco).

[0120] Experimental equipment: CO 2 Incubator (Thermo, 150i), clean bench (Suzhou Antai, SW-CJ-1F), inverted fluorescence microscope (Leica Microsystems Shanghai Co., Ltd., DMI1).

[0121] Experimental samples:

[0122]

[0123] All samples were prepared into stock solutions with a concentration of 10 mg / mL.

[0124] (3) Experimental methods:

[0125] Coating preparation and grouping:

[0126] 1. Blank group: 100uL PBS buffer was added to the 96-well plate for coating, with 3 replicates per group;

[0127] 2 Experimental group: 100uL sample solution (concentration 1mg / mL) was added to the 96-well plate for coating, with 3 replicates in each group;

[0128] At 37°C and 5% CO 2 After incubation in the incubator for 1-4 hours, remove the excess coating solution from the wells, add 100uL of 1% BSA-PBS solution, and incubate at 37°C and 5% CO 2 Continue incubating in the incubator for 1 hour. After removing the liquid in the well, wash three times with PBS, discard the washing solution, seal with sealing film, and place at 4°C for later use.

[0129] Cell recovery and culture:

[0130] Take out the P4 HFB cells from liquid nitrogen for recovery, digest them after the cells grow to 70%-80% of the culture flask, and resuspend and count them.

[0131] Adhesion Statistics:

[0132] The cells were seeded into 96-well plates at a density of 10,000 cells / well and incubated at 37°C with 5% CO. 2 After incubation in the incubator for 1-2 hours, the supernatant was aspirated and washed once with PBS, fixed with immunostaining fixative for 10 minutes and washed once with PBS, stained with Hoechst33342 staining solution and photographed. Figure 4 , and numerical statistics, see Figure 5 .

[0133] Test results and conclusions:

[0134] Compared with the control group, the cell adhesion rates of samples R8-HA (concentration 1 mg / mL), R12-HA (concentration 1 mg / mL), and R16-HA (concentration 1 mg / mL) were significantly increased, and the cell adhesion rate of sample HA-RGD20 (concentration 1 mg / mL) increased, but not strongly. This shows that samples R8-HA (concentration 1 mg / mL), HA-RGD12 (concentration 1 mg / mL), and R16-HA (concentration 1 mg / mL) can promote cell adhesion, among which R12-HA (concentration 1 mg / mL) has the best cell adhesion effect.

[0135] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0136] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A polypeptide having cell adhesion function, characterized in that: The general formula of the polypeptide sequence is: R1-(GGGG) n -RGDY-OH; Among them, R1 is sodium hyaluronate, G is glycine, R is arginine, D is aspartic acid, and Y is tyrosine.

2. A polypeptide having cell adhesion function according to claim 1, characterized in that: When n is 1 in the general formula, the polypeptide sequence is R1-GGGG-RGDY-OH.

3. The polypeptide having cell adhesion function according to claim 1, characterized in that: When n is 2 in the general formula, the polypeptide sequence is R1-GGGGGGGG-RGDY-OH.

4. The polypeptide having cell adhesion function according to claim 1, characterized in that: When n is 3 in the general formula, the polypeptide sequence is R1-GGGGGGGGGGGGG-RGDY-OH.

5. Use of the polypeptide with cell adhesion function as claimed in claim 1 in the preparation of moisturizing and repairing cosmetics.

6. The use according to claim 5, characterized in that The moisturizing repair is achieved by promoting the expression of skin barrier related genes LOR, K1, AQP3, CLDN-4 and / or FLG.

7. The use according to claim 5, characterized in that The cosmetic is one of essence, toner, lotion or cream.

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