Bactericidal composition and application thereof

Abstract

The invention relates to the technical field of biology, in particular to a bactericidal composition and application thereof. The bactericidal composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of mancozeb, 40 to 50 parts of carbendazim and 10 to 20 parts of a microbial preparation. The bactericidal composition has a very wide application prospect in biological prevention and control of sugarcane smut.

Description

[Technical field]

[0001] The present invention relates to the field of biotechnology, and in particular to a bactericidal composition and application thereof. [Background technology]

[0002] Sugarcane is a temperate and tropical crop and an important sugar cash crop in southern my country. During the planting process of sugarcane, pests and diseases will seriously hinder the growth of sugarcane. Among them, sugarcane smut is one of the important sugarcane diseases in the world and the most serious disease in my country. It is a fungal soil-borne disease. After infection, it seriously causes plant growth stagnation, reduction of effective stems, increase in fiber content, and reduction in sucrose content; it causes the sugarcane plant to show symptoms such as slender leaves, thin stems, increased tillers, and the growth of a black smut whip.

[0003] Therefore, how to prevent and control sugarcane smut and improve sugarcane yield and quality is of great research significance. [Summary of the invention]

[0004] In view of the above technical problems, the present invention aims to provide a fungicide composition and its application in preventing and controlling sugarcane smut.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A bactericidal composition comprises the following raw materials in parts by weight: 20-30 parts of zinconazole, 40-50 parts of carbendazim and 10-20 parts of a microbial preparation.

[0007] Furthermore, the bactericidal composition comprises the following raw materials in parts by weight: 25 parts of 70% mancozeb, 45 parts of 50% carbendazim and 15 parts of microbial preparation. The percentages are by mass.

[0008] Furthermore, the microbial preparation is prepared by the following method:

[0009] (1) Activation: Paenibacillus polymyxa KS-PS04 was inoculated into LB medium and cultured at 32°C for 48 h;

[0010] (2) Cultivation: The activated strain was inoculated into Gao Ze's medium No. 1, and cultured at 28-30° C. and 150-180 rpm for 24 h to obtain a seed culture solution;

[0011] (3) Fermentation: The seed culture liquid is inoculated into a fermentation tank filled with fermentation medium at an inoculation ratio of 5%, and cultured at 30-34° C. and 150-180 r / min, and sterile air is introduced for culture. The amount of sterile air introduced is 1 / 20 of the volume of the fermentation tank, until more than 95% of the bacteria in the fermentation liquid produce spores and the number of spores exceeds 1 billion / mL, and the fermentation liquid is obtained;

[0012] (4) Filter the fermentation liquid to separate the filtrate, add glucose and calcium bicarbonate to the filtrate, mix well, and then obtain a microbial preparation.

[0013] The above-mentioned Paenibacillus polymyxa KS-PS04 was isolated from sugarcane rhizosphere soil and the preservation number is CCTCC NO: M20221575; the preservation location is: China Center for Type Culture Collection, Wuhan University, Wuhan, China, and the preservation time is October 17, 2022.

[0014] The present invention also includes a method for controlling sugarcane smut using the above-mentioned fungicide composition, wherein the method comprises: before planting sugarcane, firstly treating sugarcane seeds as follows: soaking the sugarcane seeds in the fungicide composition for 8-12 minutes.

[0015] Furthermore, the sugarcane seeds are selected from double-bud sugarcane seeds, and are cut by an oblique knife.

[0016] The present invention has the following beneficial effects:

[0017] The fungicidal composition of the present application is mainly composed of zinc manganese sulfamethoxam, carbendazim and a microbial preparation. The microbial preparation is prepared by activating and culturing the strain KS-PS04. The formula after the above compounding can effectively prevent and control sugarcane smut. The development of the fungicidal composition reduces the prevention and control costs and pesticide residues, and is of great significance for the development of new agents for preventing and controlling sugarcane smut. [Specific implementation method]

[0018] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below.

[0019] All features disclosed in this specification, or steps in all methods or processes disclosed, except mutually exclusive features and / or steps, can be combined in any manner.

[0020] Any feature disclosed in this specification (including any appended claims and abstract), unless otherwise stated, is merely an example of a series of equivalent or similar features.

[0021] Embodiment 1:

[0022] The strain of Paenibacillus polymyxa KS-PS04 in this example has a deposit number of CCTCC NO: M20221575 and a Latin name of Paenibacillus polymyxa. The deposit location is: Wuhan University, Wuhan, China; China Center for Type Culture Collection, and the deposit time is October 17, 2022.

[0023] The above strains were collected from the soil around the sugarcane roots in the sugarcane field of Zhangsi Village, Quanfeng Village, Jiangzhou District, Chongzuo City, Guangxi Zhuang Autonomous Region, and isolated using the trapping method.

[0024] Embodiment 2:

[0025] This embodiment provides a bactericidal composition, which comprises the following raw materials in parts by weight: 20 parts of 70% mancozeb, 40 parts of 50% carbendazim and 10 parts of a microbial preparation.

[0026] Wherein, the microbial preparation is prepared by the following method:

[0027] (1) Activation: Paenibacillus polymyxa KS-PS04 was inoculated into LB medium and cultured at 32°C for 48 h;

[0028] (2) Cultivation: The activated strain was inoculated into Gao Ze's medium No. 1, and cultured at 28° C. and 150 rpm for 24 h to obtain a seed culture solution;

[0029] (3) Fermentation: The seed culture solution is inoculated into a fermentation tank filled with fermentation medium at an inoculation ratio of 5%, and cultured at 30° C. and 150 r / min, with sterile air introduced for culture, wherein the amount of sterile air introduced is 1 / 20 of the volume of the fermentation tank, until more than 95% of the bacteria in the fermentation solution produce spores and the number of spores exceeds 1 billion / mL, thereby obtaining a fermentation solution;

[0030] (4) Filter the fermentation liquid to separate the filtrate, add glucose and calcium bicarbonate to the filtrate, mix well, and then obtain a microbial preparation.

[0031] Embodiment 3:

[0032] This embodiment provides a bactericidal composition, which comprises the following raw materials in parts by weight: 25 parts of 70% mancozeb, 45 parts of 50% carbendazim and 15 parts of a microbial preparation.

[0033] Wherein, the microbial preparation is prepared by the following method:

[0034] (1) Activation: Paenibacillus polymyxa KS-PS04 was inoculated into LB medium and cultured at 32°C for 48 h;

[0035] (2) Cultivation: The activated strain was inoculated into Gao Ze's medium No. 1, and cultured at 29° C. and 160 rpm for 24 h to obtain a seed culture solution;

[0036] (3) Fermentation: The seed culture solution is inoculated into a fermentation tank filled with fermentation medium at an inoculation ratio of 5%, and cultured at 32° C. and 160 r / min, with sterile air introduced for culture, wherein the amount of sterile air introduced is 1 / 20 of the volume of the fermentation tank, until more than 95% of the bacteria in the fermentation solution produce spores and the number of spores exceeds 1 billion / mL, thereby obtaining a fermentation solution;

[0037] (4) Filter the fermentation liquid to separate the filtrate, add glucose and calcium bicarbonate to the filtrate, mix well, and then obtain a microbial preparation.

[0038] Embodiment 4:

[0039] This embodiment provides a bactericidal composition, which comprises the following raw materials in parts by weight: 30 parts of 70% mancozeb, 50 parts of 50% carbendazim and 20 parts of a microbial preparation.

[0040] Wherein, the microbial preparation is prepared by the following method:

[0041] (1) Activation: Paenibacillus polymyxa KS-PS04 was inoculated into LB medium and cultured at 32°C for 48 h;

[0042] (2) Cultivation: The activated strain was inoculated into Gao Ze's medium No. 1, and cultured at 30° C. and 180 rpm for 24 h to obtain a seed culture solution;

[0043] (3) Fermentation: The seed culture solution is inoculated into a fermentation tank filled with fermentation medium at an inoculation ratio of 5%, and cultured at 34° C. and 180 r / min, with sterile air introduced for culture, wherein the amount of sterile air introduced is 1 / 20 of the volume of the fermentation tank, until more than 95% of the bacteria in the fermentation solution produce spores and the number of spores exceeds 1 billion / mL, thereby obtaining a fermentation solution;

[0044] (4) Filter the fermentation liquid to separate the filtrate, add glucose and calcium bicarbonate to the filtrate, mix well, and then obtain a microbial preparation.

[0045] Embodiment 5:

[0046] Effect on the prevention and control of sugarcane smut:

[0047] The experiment was conducted from January to October 2023 at the Zhangshitun Experimental Base in Quanfeng Village, Leshui Town, Jiangzhou District, Chongzuo City, Guangxi Zhuang Autonomous Region.

[0048] The test method is as follows: before planting sugarcane, the sugarcane seeds are first treated as follows: the sugarcane seeds are soaked in the treatment solution (diluted 200 times) for 10 minutes. The sugarcane seeds are selected as double-bud sugarcane seeds, and the seeds are cut by an oblique knife.

[0049] In order to test the effect, the applicant used different treatment liquids for immersion treatment, which were divided into the following groups:

[0050] The first group: the treatment solution described in Example 3;

[0051] The second group: the composition weight parts of the bactericidal composition are: 25 parts of 70% mancozeb, 45 parts of 50% carbendazim and 3 parts of microbial preparation;

[0052] Group 3: Remove the microbial preparation ingredient;

[0053] Group 4: Remove the ingredient of zinc manganese;

[0054] Group 5: Remove the ingredient carbendazim;

[0055] Group 6: only microbial preparations were used as treatment fluid;

[0056] Group 7: Blank control group: soaked in clean water.

[0057] The incidence of smut during the planting period of the sugarcane seeds treated with the above-mentioned treatment solutions was compared. Except for the differences emphasized above, the other cultivation methods were the same. The comparison results are recorded in Table 1:

[0058] Table 1 Incidence of sugarcane smut under different treatments

[0059] Smut incidence (%) Group 1 11.38％ Group 2 23.82％ Group 3 28.11％ Group 4 21.69％ Group 5 30.01％ Group 6 29.83％ Group 7 45.26％

[0060] According to the results in Table 1, it can be seen that soaking sugarcane seeds with the fungicide composition of the present application can effectively reduce the incidence of smut. The development of the fungicide composition reduces the cost of prevention and control and the residue of pesticides, and is of great significance for the development of new agents for preventing and controlling sugarcane smut.

[0061] The above description is a detailed description of the preferred feasible embodiments of the present invention, but the embodiments are not intended to limit the scope of the patent application of the present invention. All equivalent changes or modified changes completed under the technical spirit suggested by the present invention should fall within the patent scope covered by the present invention.

Claims

1. A bactericidal composition, characterized in that: The bactericidal composition comprises the following raw materials in parts by weight: 20-30 parts of zinconazole, 40-50 parts of carbendazim and 10-20 parts of microbial preparation.

2. The bactericidal composition according to claim 1, characterized in that The bactericidal composition comprises the following raw materials in parts by weight: 25 parts of 70% mancozeb, 45 parts of 50% carbendazim and 15 parts of microbial preparation.

3. The bactericidal composition according to claim 1, characterized in that The microbial preparation is prepared by the following method: (1) Activation: Paenibacillus polymyxa KS-PS04 was inoculated into LB medium and cultured at 32°C for 48 h; (2) Cultivation: The activated strain was inoculated into Gao Ze's medium No. 1, and cultured at 28-30° C. and 150-180 rpm for 24 h to obtain a seed culture solution; (3) Fermentation: The seed culture liquid is inoculated into a fermentation tank filled with fermentation medium at an inoculation ratio of 5%, and cultured at 30-34° C. and 150-180 r / min, and sterile air is introduced for culture. The amount of sterile air introduced is 1 / 20 of the volume of the fermentation tank, until more than 95% of the bacteria in the fermentation liquid produce spores and the number of spores exceeds 1 billion / mL, and the fermentation liquid is obtained; (4) Filter the fermentation liquid to separate the filtrate, add glucose and calcium bicarbonate to the filtrate, mix well, and then obtain a microbial preparation.

4. Use of the fungicidal composition according to any one of claims 1 to 3 in controlling sugarcane smut.

5. A method for preventing and controlling sugarcane smut, characterized in that: The method comprises the following steps: before planting sugarcane, firstly treating sugarcane seeds as follows: soaking the sugarcane seeds in a bactericidal composition for 8-12 minutes.

6. The method according to claim 5, characterized in that The sugarcane seeds are selected from double-bud sugarcane seeds, and the seeds are cut by an oblique knife.