Fermented sunflower seed oil composition and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a fermented sunflower seed oil composition and application thereof. The composition comprises an astragalus membranaceus root extract, an American ginseng root extract, a salvia miltiorrhiza root extract, a ginseng root extract and sunflower seed oil. The sunflower seed oil, turmeric, astragalus membranaceus, American ginseng, salvia miltiorrhiza and ginseng are mixed and fermented to obtain the sunflower seed oil fermentation composition. Through microbial action, active ingredients in plants are fully released and are converted into micromolecular substances which are easier to absorb, and the composition has better anti-wrinkle, soothing and other effects after being added into cosmetics.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, in particular to a fermented sunflower seed oil composition and application thereof. Background Art

[0002] Most of the active ingredients extracted from cosmetic plants are water-soluble. However, a large part of the active ingredients in plants are insoluble in water and are oil-soluble. There are also many oil-soluble plant extracts on the market. However, due to their large molecular weight of active substances, they are not easily absorbed by human skin.

[0003] Fermentation is the process of preparing microbial cells themselves, or direct metabolites or secondary metabolites through life activities under aerobic or anaerobic conditions.

[0004] Sunflower oil, also known as sunflower oil or sunflower seed oil, is the oil extracted from sunflower seeds and is commonly used in cooking and cosmetics as an emollient. Sunflower has been shown to effectively relieve many skin conditions, including dryness, acne, and sun damage. Its benefits come from a compound called beta-carotene, which is commonly found in fruits and vegetables. In addition, sunflower oil is rich in antioxidants, which can help remove free radicals and toxins that cause certain skin problems due to cell damage.

[0005] However, one drawback of vegetable oil as a skin care product is that the temperature difference between hot and cold can cause the oily substances to precipitate, which in turn leads to problems with the stability, skin feel, and efficacy of the product.

[0006] To address this, the present invention provides a fermented sunflower seed oil composition and application thereof. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a fermented sunflower oil composition, which comprises:

[0008] Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, Helianthus annuus seed oil.

[0009] As an embodiment of the present invention, the mass ratio of the Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, and sunflower seed oil is (15-20): (0.5-1.5): (1-3): (18-22): (40-55).

[0010] As an embodiment of the present invention, the mass ratio of the Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, and sunflower seed oil is 18:0.9:2:20:50.1.

[0011] As an embodiment of the present invention, the composition further comprises turmeric root extract and jojoba oil.

[0012] As an embodiment of the present invention, the preparation method of the composition is as follows:

[0013] S01 Wash, dry, crush, mix and soak the roots of Astragalus membranaceus, American ginseng, Salvia miltiorrhiza, ginseng and turmeric respectively, and soak them in a 60% ethanol solution for 48 hours; filter to obtain filtrate A and residue A; S02 mix the residue A and culture medium A, inoculate strain A for fermentation, and filter to obtain residue B;

[0014] S03: Mix the filter residue B with sunflower seed oil and jojoba oil for fermentation, centrifuge the final fermentation product at 4000 r / min for 30 min, and take the supernatant to obtain a fermented sunflower seed oil composition;

[0015] The solvent of S04 filtrate A is evaporated at a constant temperature of 30°C until its mass is half of that of the plant raw material and then set aside.

[0016] An emulsion comprises the fermented sunflower seed oil composition.

[0017] As an embodiment of the present invention, the raw materials for preparing the emulsion include:

[0018] Deionized water, PEG-100 stearate, glyceryl stearate, cetearyl alcohol, cetearyl glucoside, PEG-20 methyl glucose sesquistearate, cetearyl alcohol, polydimethylsiloxane, ethylhexyl palmitate, squalane, glycerin, 1,3-propylene glycol, carbomer, arginine, 1,2-hexanediol, p-hydroxyacetophenone, and the fermented sunflower oil composition according to any one of claims 1 to 5.

[0019] As an embodiment of the present invention, the raw materials for preparing the emulsion include: 77.3% deionized water, PEG-100 stearate, 2% glyceryl stearate, 0.2% cetearyl alcohol, 0.3% cetearyl glucoside, 0.8% PEG-20 methyl glucose sesquistearate, 1% cetearyl alcohol, 2% polydimethylsiloxane, 5% ethylhexyl palmitate, 3% squalane, 3% glycerol, 2% 1,3-propylene glycol, 0.2% carbomer, 0.2% arginine, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and 2% fermented sunflower oil composition.

[0020] As an embodiment of the present invention, the preparation method of the emulsion comprises the following steps:

[0021] (1) Preparing phase 1: Take a small amount of deionized water and dissolve arginine evenly for later use;

[0022] (2) Preparing phase 2: Dissolve 1,3-propylene glycol and p-hydroxyacetophenone in a water bath at 70° C. with stirring until completely dissolved and set aside;

[0023] (3) Add deionized water to a beaker, and then add glycerol, carbomer, and filtrate A in the fermented sunflower seed oil composition in sequence, heat to 80-85°C, and stir at 800rpm-1000rpm for 10min-20min until uniform; take another beaker and add PEG-100 stearate, glyceryl stearate, cetearyl alcohol, cetearyl glucoside, PEG-20 methyl glucose sesquistearate, polydimethylsiloxane, ethylhexyl palmitate, and squalane in sequence, heat to 80-82°C, and stir to dissolve completely; add the oil phase to the water phase and homogenize at 2500rpm-3000rpm with a homogenizer for 3min-5min for emulsification, cool after emulsification, and wait until the material body is cooled to 45-50°C, then add preformed phase 1, preformed phase 2, 1,2-hexanediol, and the fermented sunflower seed oil composition in sequence, and stir to uniformity.

[0024] As an embodiment of the present invention, the fermented sunflower seed oil composition is used in the field of skin care.

[0025] By adopting the above technical solution, the present invention has the following beneficial effects:

[0026] The present invention mixes sunflower oil with turmeric, astragalus, American ginseng, salvia miltiorrhiza and ginseng to obtain a sunflower oil fermentation composition. Through the action of microorganisms, the active ingredients in the plants are fully released and converted into small molecules that are easier to absorb. When added to cosmetics, the composition has good anti-wrinkle and soothing effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 The emulsion obtained in Example 5. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The present invention provides a fermented sunflower seed oil composition, which comprises:

[0031] Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, Helianthus annuus seed oil.

[0032] As an embodiment of the present invention, the composition further comprises turmeric root extract and jojoba oil.

[0033] As an embodiment of the present invention, the preparation method of the composition is as follows:

[0034] S01 Wash, dry, crush and mix the roots of Astragalus membranaceus, American ginseng, Salvia miltiorrhiza, ginseng and turmeric respectively, soak them in a 60% ethanol solution at a solid-liquid ratio of 1:4 for 48 hours; filter to obtain filtrate A and residue A;

[0035] S02: mixing the filter residue A and the culture solution A, inoculating the strain A for fermentation, and filtering to obtain the filtrate B and the filter residue B;

[0036] The components of culture medium A are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, and 1000.0 mL of distilled water.

[0037] Bacterial strain A is Bacillus subtilis, China Industrial Microbiological Culture Collection Management Center, 21322; the inoculation amount is 3wt%.

[0038] S03: Mix the filter residue B with sunflower seed oil and jojoba oil for fermentation, centrifuge the final fermentation product at 4000 r / min for 30 min, and take the supernatant to obtain a fermented sunflower seed oil composition;

[0039] Strain B is Pichia jadinii, from China Industrial Microbiological Culture Collection Center, 1258, the inoculation amount was 2wt%.

[0040] Culture medium B is composed of 1% glucose, 0.5% peptone, 0.3% yeast extract, 0.0135% citric acid, 0.126% sodium citrate, and the balance water.

[0041] S04 filtrate A and filtrate B are mixed and the solvent is evaporated at a constant temperature of 30°C until the mass is half of the mass of the plant raw material, and then set aside.

[0042] The present invention is further explained below in conjunction with specific implementation modes.

[0043] Example 1

[0044] The present embodiment provides a fermented sunflower seed oil composition, which comprises: Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, sunflower seed oil, Curcuma longa root extract and jojoba oil; and the mass ratio is: 18:0.9:2:20:50.1:9:1.

[0045] In this embodiment, the preparation method of the fermented sunflower oil composition is as follows:

[0046] S01 Wash, dry, crush and mix the roots of Astragalus membranaceus, American ginseng, Salvia miltiorrhiza, ginseng and turmeric respectively, soak them in a 60% ethanol solution at a solid-liquid ratio of 1:4 for 48 hours; filter to obtain filtrate A and residue A;

[0047] S02: Mix the filter residue A and the culture solution A, inoculate the strain A for fermentation, and then filter out the filtrate B and the filter residue B; the mass ratio of the filter residue A to the culture solution A is 1:15; the fermentation condition is 30°C for 36 hours;

[0048] The components of culture medium A are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, and 1000.0 mL of distilled water.

[0049] Bacterial strain A is Bacillus subtilis, China Industrial Microbiological Culture Collection Management Center, 21322; the inoculation amount is 3wt%.

[0050] S03: Mix the filter residue B with sunflower seed oil and jojoba oil for fermentation, centrifuge the final fermentation product at 4000 r / min for 30 min, and take the supernatant to obtain a fermented sunflower seed oil composition;

[0051] In S03, strain B is Pichia jadinii, which is from China Industrial Microbiological Culture Collection Center. 1258, the inoculation amount was 2wt%.

[0052] The culture medium B is composed of 1% glucose, 0.5% peptone, 0.3% yeast extract, 0.0135% citric acid, 0.126% sodium citrate, and the balance water.

[0053] The mass ratio of the residue B to sunflower seed oil, jojoba oil and culture medium B is 1:10.

[0054] S04 filtrate A and filtrate B are mixed and the solvent is evaporated at a constant temperature of 30°C until the mass is half the mass of the plant raw material, which is filtrate concentrate C for use.

[0055] Example 2

[0056] The difference from Example 1 is that the composition does not contain: turmeric root extract and jojoba oil.

[0057] Example 3

[0058] The difference from Example 1 is that strain A is replaced by

[0059] Example 4

[0060] The difference from Example 1 is that the preparation method of the fermented sunflower seed oil composition is as follows:

[0061] S01 Wash, dry, crush and mix the roots of Astragalus membranaceus, American ginseng, Salvia miltiorrhiza, ginseng and turmeric respectively, soak them in a 60% ethanol solution at a solid-liquid ratio of 1:4 for 48 hours; filter to obtain a filtrate and a filter residue;

[0062] S02: mixing the filter residue in S01 with sunflower seed oil and jojoba oil for fermentation, centrifuging the final fermentation product at 4000 r / min for 30 min, and taking the supernatant to obtain a fermented sunflower seed oil composition;

[0063] In S02, the strain is Pichia jadinii, which is from the China Industrial Microbiological Culture Collection Center. 1258, the inoculation amount was 2wt%.

[0064] The culture medium is composed of 1% glucose, 0.5% peptone, 0.3% yeast extract, 0.0135% citric acid, 0.126% sodium citrate and the balance water.

[0065] The mass ratio of the filter residue to sunflower seed oil, jojoba oil and culture medium is 1:10.

[0066] S03 The filtrate in S01 is subjected to solvent evaporation at a constant temperature of 30°C until its mass is half that of the plant material, which is filtrate concentrate C for later use.

[0067] Example 5

[0068] The present embodiment provides an emulsion, and the raw materials for preparing the emulsion include: 77.3% deionized water, PEG-100 stearate, 2% glyceryl stearate, 0.2% cetearyl alcohol, 0.3% cetearyl glucoside, 0.8% PEG-20 methyl glucose sesquistearate, 1% cetearyl alcohol, 2% polydimethylsiloxane, 5% ethylhexyl palmitate, 3% squalane, 3% glycerol, 2% 1,3-propylene glycol, 0.2% carbomer, 0.2% arginine, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and 2% of the fermented sunflower oil composition described in Example 1.

[0069] This embodiment also provides a method for preparing an emulsion, the steps are as follows:

[0070] (1) Preparing phase 1: Take a small amount of deionized water and dissolve arginine evenly for later use;

[0071] (2) Preparing phase 2: Dissolve 1,3-propylene glycol and p-hydroxyacetophenone in a water bath at 70° C. with stirring until completely dissolved and set aside;

[0072] (3) Add deionized water to a beaker, and then add glycerol and carbomer in sequence. The filtrate concentrate C in the fermented sunflower oil composition in Example 1 is heated to 80-85°C, and stirred at 800rpm-1000rpm for 10min-20min until uniform; take another beaker and add PEG-100 stearate, glyceryl stearate, cetearyl alcohol, cetearyl glucoside, PEG-20 methyl glucose sesquistearate, polydimethylsiloxane, ethylhexyl palmitate, and squalane in sequence, heat to 80-82°C, and stir to dissolve completely; add the oil phase to the water phase and homogenize at 2500rpm-3000rpm with a homogenizer for 3min-5min for emulsification, cool after emulsification, and wait until the material body is cooled to 45-50°C, then add prefabricated phase 1, prefabricated phase 2, 1,2-hexanediol, and the fermented sunflower oil composition obtained in step S03 in Example 1 in sequence, and stir evenly.

[0073] The difference between Example 6 and Example 5 is that Example 6 does not contain the fermented sunflower oil composition of Example 1, but contains the fermented sunflower oil composition of Example 2.

[0074] The difference between Example 7 and Example 5 is that Example 7 does not contain the fermented sunflower oil composition of Example 1, but contains the fermented sunflower oil composition of Example 3.

[0075] The difference between Example 8 and Example 5 is that Example 8 does not contain the fermented sunflower oil composition of Example 1, but contains the fermented sunflower oil composition of Example 4.

[0076] The difference between Example 9 and Example 5 is that the filtrate concentrate C in the fermented sunflower seed oil composition of Example 1 is not included.

[0077] Performance Testing

[0078] Test 1: The emulsions prepared in Examples 5 to 9 were placed at -15°C, 5°C and 48°C for 90 days to observe the properties of the emulsions; the specific results are shown in Table 1.

[0079] Test 2: The emulsions prepared in Examples 5 to 9 were placed at 0°C for 12 h, and then at 48°C for 12 h. This cycle was repeated 5 times to check the product properties. The specific results are shown in Table 1.

[0080] Table 1 Test results of performance test 1 and test 2

[0081]

[0082] Test 3: Human anti-wrinkle and firming efficacy evaluation test

[0083] Take an appropriate amount of this product and apply it evenly to the skin around the eyes, and gently massage along the skin texture around the eyes until absorbed. Frequency of use: once in the morning and once in the evening.

[0084] 2.1 Average depth, number, volume and length of crow's feet

[0085] Test site: end of eye on one side

[0086] Test instrument Skin fast 3D imaging system PRIMOS-CR

[0087] Test time: Before using the sample, 28 days after using the sample

[0088] Parameter Description The smaller the measured value, the smaller the average depth, number, volume and length of crow's feet

[0089] 2.2 Area ratio of crow's feet

[0090] Test site: One side of the eye

[0091] Test method: Facial image analyzer VISIA-CR, image analysis software Image-ProPlus

[0092] Test time: before using the sample, 28 days after using the sample

[0093] Parameter description: The smaller the measured value, the fewer crow's feet wrinkles there are.

[0094] 2.3 Skin elasticity R2 value

[0095] Test area: Skin around the eye on one side

[0096] Test method Courage+Khazaka Skin elasticity tester Cutometer

[0097] Test time: Before using the sample, 28 days after using the sample

[0098] Parameter Description: The larger the measurement value, the better the skin elasticity.

[0099] 2.4 Skin firmness F4 value

[0100] Test area: Skin around the eye on one side

[0101] Test method Courage+Khazaka Skin Elasticity Tester Cutometer MPA580

[0102] Test time: Before using the sample, 28 days after using the sample

[0103] Parameter Description The smaller the measurement value, the higher the skin firmness.

[0104] Number of subjects: 30 participants, 30 participants were actually included, and the effective rate was 100%

[0105] Gender of subjects: 5 males, 25 females

[0106] Age of subjects: 36 to 60 years old, average 47.63 years old

[0107] Test area: Corner of eye

[0108] Test environment: temperature 21±1℃, relative humidity 50±10%RH

[0109] Testing period: 28 days

[0110] Testing Process

[0111] Before using the sample

[0112] One week before the test, the subjects stopped using facial cosmetics or topical medications. The night before the test, the subjects did not apply any skin care products on their faces after washing their faces at home. On the morning of the test, the subjects did not apply any skin care products on their faces after washing their faces with clean water at home. The qualified subjects filled out the informed consent form. The subjects cleaned their faces with clean water and rested in a constant temperature and humidity chamber (temperature 21±1℃, relative humidity 50±10%RH) for 30 minutes. The PRIMOS-CR photo test was performed. The photos were taken using VISIA-7. The skin elasticity tester Cutometer was used to measure the skin elasticity R2 value and skin firmness F4 value. The test samples and instructions for use were distributed.

[0113] After using the sample for 28 days

[0114] After cleaning their faces with clean water, the subjects rested in a constant temperature and humidity chamber (temperature 21±1°C, relative humidity 50±10% RH) for 30 minutes; performed the PRIMOS-CR test; took photos using VISIA-7; used a skin elasticity tester Cutometer to measure the skin elasticity R2 value and skin firmness F4 value; and the subjects performed self-assessment.

[0115] Table 2 Test results of test 3

[0116]

[0117] Improvement rate of crow's feet wrinkles = (average value before use - average value after use) / (average value before use) * 100%

[0118] Skin elasticity R2 value improvement rate = (average after use - average before use) / (average before use) * 100% Skin firmness F4 value improvement rate = (average before use - average after use) / (average before use) * 100% Test 4: Human patch test

[0119] 1. Materials and Methods

[0120] 1. Test substance: dilute the final product with distilled water to a concentration of 1%

[0121] 2. Negative control: distilled water control. 3. Subjects: 30 subjects, 2 males and 28 females, aged 24 to 60 years old, with an average age of 45.20±11.91 years old, who met the voluntary inclusion criteria.

[0122] 4. Patch test method: Select qualified patch test equipment and use the closed patch test method. Place about 0.020mL~0.025ml of the test substance in the patch tester, and apply it to the back of the subject with low-allergenic tape. Remove the test substance after 24 hours, and observe the skin reaction 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0123] Table 3 Summary of cosmetics human skin patch test results

[0124]

[0125] Test 5: Hyaluronidase inhibition rate

[0126] Hyaluronidase activity inhibition test (soothing)

[0127] Hyaluronidase is a hyaluronic acid hydrolase. Hyaluronic acid is an acidic mucopolysaccharide widely present in the human body, animals and plants. It is an important component of the extracellular matrix in the human body and is more present in connective tissue, skin, etc. Adding hyaluronidase inhibitors to cosmetics can increase the relative level of hyaluronic acid, keep the skin moisturized, and have an anti-allergic effect. This experiment uses a double antibody sandwich method to detect the inhibition rate of samples on hyaluronidase. The inhibitory factors that may exist in the sample inhibit the activity of hyaluronidase. The still active hyaluronidase binds to the biotin-labeled antibody and then to streptavidin-HRP to form an immune complex, incubate, wash, and then add the color developing solution to develop color. After the reaction is terminated, the absorbance value is tested by an enzyme marker. At the same time, a control group and a blank group are made. By comparing and analyzing the test data, the inhibitory effect of the sample on hyaluronidase is evaluated. From this data, the soothing effect of cosmetics can be relatively reasonably evaluated.

[0128] Prepare Examples 5 to 9, a control group, and a blank group, add samples and hyaluronidase to the corresponding wells of the sample group and the control group, and incubate at 37° C. for 10 minutes; then add biotin-labeled antibodies and enzyme-labeled reagents to the corresponding wells of the test group and the control group, incubate at 37° C. for 60 minutes, wash the plate 5 times, pat dry, add color developing solutions A and B to all wells, develop at 37° C. for 15 minutes, and add stop solution; within 10 minutes, test the OD value at 450 nm with an enzyme reader, and calculate the hyaluronidase activity inhibition rate.

[0129] Sample inhibition rate = (average absorbance of the control group - average absorbance of the sample group) / (average absorbance of the control group - average absorbance of the blank group) * 100%. The test results are shown in Table 4.

[0130] Table 4 Test results

[0131] Example Inhibition rate / % Example 5 66.842±0.673 Example 6 57.245±1.863 Example 7 62.472±1.164 Example 8 59.163±0.934 Example 9 55.746±1.148

[0132] Test 6: Elastase inhibition rate

[0133] Elastase test

[0134] The anti-wrinkle and firming effects are mainly characterized by evaluating the inhibition rate of test samples on elastase. Elastase is mainly synthesized and secreted by fibroblasts, and can degrade elastin in the skin, leading to skin aging. The experimental principle of inhibiting elastase is that porcine pancreatic elastase catalyzes the reaction with the enzyme substrate. After adding active substances, the absorbance changes, and the size of the absorbance change reflects the inhibition rate of the elastase inhibitor.

[0135] Reagents

[0136] Elastase (Porcine Pancreas), BR

[0137] N-Succinyl L-Alanyl I-Alanyl L-Alanine, 98% (EGCG), 98% Epigallocatechin Gallate

[0138] Handling of control and test samples

[0139] Emulsion: Dilute with pure water to a sample concentration of 0.1%:

[0140] Positive control (EGCG): Dilute with water to a positive control concentration of 0.1%:

[0141] Negative control: pure water.

[0142] Test operation steps

[0143] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions to the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0144] Elastase inhibition rate %=(1-(CD) / (AB))*100.

[0145] Wherein: A- is the absorbance of the reaction solution without sample; B- is the absorbance of the reaction solution without sample and enzyme; C- is the absorbance of the reaction solution containing sample and enzyme; D- is the absorbance of the reaction solution containing sample and no enzyme.

[0146] Table 5 Test results of test 6

[0147] Example Inhibition rate / % Example 5 51.505±1.862 Example 6 41.505±1.862 Example 7 48.254±1.146 Example 8 46.286±0.782 Example 9 40.012±1.363 Positive control 81.143±1.145 Negative control -6.932±2.452

[0148] Test 7: DPPH free radical scavenging rate

[0149] The determination is carried out by colorimetry. Principle of determination: DPPH free radical has a single electron, which makes it have a very strong absorption at 525nm (dark purple). When there is a free radical scavenger, the absorption gradually disappears due to pairing with its single electron. The degree of fading is quantitatively related to the number of electrons it receives, so it can be quantitatively analyzed by spectrophotometry.

[0150] Method: Take 2.0mL of 120μmol / L DPPH free radical solution and add it to 20μL of the sample to be tested. Use the same dose of double distilled water instead of the sample to add the same volume of DPPH free radical solution as the control (A0). Use 2.0mL of 50% ethanol instead of DPPH free radical solution to adjust to zero. After placing at 25℃ for 20min, measure the absorbance of the DPPH free radical mixed solution at 520nm (sample A). The scavenging ability is expressed as the scavenging rate. Repeat 3 times and calculate according to the following formula:

[0151] DPPH free radical scavenging rate % = (A0-A 样 )×100 / A0

[0152] Table 6 Test results of test 7

[0153] Example Inhibition rate / % Example 5 58.941±2.276 Example 6 50.159±1.043 Example 7 57.261±0.836 Example 8 56.739±1.036 Example 9 49.342±2.537 Control group 6.583±0.843

[0154] The present invention adopts a method of first fermenting and extracting, then adding an oily composition, and then adding yeast for extraction, so as to prepare a component with high product stability. The product is not easy to separate, and the product prepared by matching with polydimethylsiloxane has a better effect. The product obtained by the fermentation step of the present invention has a small molecular weight and better product absorbency.

[0155] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fermented sunflower oil composition, characterized in that: The composition comprises: Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, Helianthus annuus seed oil.

2. A fermented sunflower oil composition according to claim 1, characterized in that: The mass ratio of the Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract and sunflower seed oil is (15-20): (0.5-1.5): (1-3): (18-22): (40-55).

3. A fermented sunflower oil composition according to claim 1, characterized in that: The mass ratio of the Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract and sunflower seed oil is 18:0.9:2:20:50.

1.

4. A fermented sunflower seed oil composition according to any one of claims 1 to 3, characterized in that: The composition also includes turmeric root extract and jojoba oil.

5. A fermented sunflower oil composition according to any one of claim 4, characterized in that: The preparation method of the composition is as follows: S01 Wash, dry, crush, mix, and soak the Astragalus membranaceus root extract, American ginseng root extract, Salvia miltiorrhiza root extract, Panax ginseng root extract, and Curcuma longa root extract in 60% ethanol solution for 48 hours; filter to obtain filtrate A and residue A; S02: mixing the filter residue A and the culture solution A, inoculating the strain A for fermentation, and filtering to obtain the filter residue B; S03: Mix the filter residue B with sunflower seed oil and jojoba oil for fermentation, centrifuge the final fermentation product at 4000 r / min for 30 min, and take the supernatant to obtain a fermented sunflower seed oil composition; The solvent of S04 filtrate A is evaporated at a constant temperature of 30°C until its mass is half of that of the plant raw material and then set aside.

6. An emulsion, characterized in that A fermented sunflower seed oil composition comprising any one of claims 1 to 5.

7. An emulsion according to claim 6, characterized in that The raw materials for preparation include: Deionized water, PEG-100 stearate, glyceryl stearate, cetearyl alcohol, cetearyl glucoside, PEG-20 methyl glucose sesquistearate, cetearyl alcohol, polydimethylsiloxane, ethylhexyl palmitate, squalane, glycerin, 1,3-propylene glycol, carbomer, arginine, 1,2-hexanediol, p-hydroxyacetophenone, and the fermented sunflower oil composition according to any one of claims 1 to 5.

8. An emulsion according to claim 6, characterized in that The preparation raw materials include: 77.3% deionized water, PEG-100 stearate, 2% glyceryl stearate, 0.2% cetearyl alcohol, 0.3% cetearyl glucoside, 0.8% PEG-20 methyl glucose sesquistearate, 1% cetearyl alcohol, 2% polydimethylsiloxane, 5% ethylhexyl palmitate, 3% squalane, 3% glycerol, 2% 1,3 propanediol, 0.2% carbomer, 0.2% arginine, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and 2% fermented sunflower oil composition.

9. A method for preparing an emulsion according to any one of claims 6 to 8, characterized in that: Here are the steps: (1) Preparing phase 1: Take a small amount of deionized water and dissolve arginine evenly for later use; (2) Preparing phase 2: Dissolve 1,3-propylene glycol and p-hydroxyacetophenone in a water bath at 70° C. with stirring until completely dissolved and set aside; (3) Add deionized water to a beaker, and then add glycerol, carbomer, and filtrate A in the fermented sunflower seed oil composition in sequence, heat to 80-85°C, and stir at 800rpm-1000rpm for 10min-20min until uniform; take another beaker and add PEG-100 stearate, glyceryl stearate, cetearyl alcohol, cetearyl glucoside, PEG-20 methyl glucose sesquistearate, polydimethylsiloxane, ethylhexyl palmitate, and squalane in sequence, heat to 80-82°C, and stir to dissolve completely; add the oil phase to the water phase and homogenize at 2500rpm-3000rpm with a homogenizer for 3min-5min for emulsification, cool after emulsification, and wait until the material body is cooled to 45-50°C, then add preformed phase 1, preformed phase 2, 1,2-hexanediol, and the fermented sunflower seed oil composition in sequence, and stir to uniformity.

10. The fermented sunflower oil composition according to any one of claims 1 to 5, characterized in that: Used in skin care field.

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