Method for planting folium isatidis in severe saline-alkali soil

Abstract

The invention relates to the technical field of cultivation and planting of medicinal plants, in particular to a method for planting folium isatidis in severe saline-alkali soil. The method comprises the following steps: (1) soil preparation; (2) applying a base fertilizer: spreading 10-20 kg / mu of humic acid, 1-5 kg / mu of charcoal, 2000-4000 kg / mu of a microbial organic fertilizer, 100-150 kg / mu of waste mushroom sticks and 10-15 kg / mu of diammonium phosphate on the surface of the soil, and deeply plowing for 25-30 cm for two times; (3) ridging and sowing; (4) field management; (5) pest control; (6) harvesting. Through reasonable base fertilizer application and topdressing, the survival rate and yield of folium isatidis in the severe saline-alkali soil are increased, especially the content of effective components such as indirubin is increased, and high economic benefits are obtained while the severe saline-alkali soil is utilized.

Description

Technical Field

[0001] The invention relates to the technical field of cultivation and planting of medicinal plants, in particular to a method for planting Folium Isatidis in severe saline-alkali land. Background Art

[0002] Isatis root is the dried root of the cruciferous plant Isatis indigotica, also known as grass isatis; the leaves of isatis root are called isatis indigotica leaves, and the dark blue powder made from the fresh leaves is indigo. Both are commonly used Chinese medicines with similar properties, and have the functions of clearing away heat and detoxifying, cooling blood and reducing swelling. Currently, the wild resources of isatis root are becoming increasingly scarce, and the quality of large-scale artificial cultivation is uneven, making it difficult to meet the growing market demand.

[0003] As a special land resource, saline-alkali land has a decrease in soil fertility due to excessive salt and alkali, which seriously affects the normal growth of crops. Isatis root has a strong salt-alkali tolerance and is highly adaptable to the growth environment of saline-alkali land. Moreover, due to the appropriate salinity and alkalinity providing an "environmental stress" effect for Isatis root and Isatis indigotica, the plant will increase the synthesis of secondary metabolites such as alcohols, terpenes and various vitamins, thereby increasing the active medicinal ingredients of Isatis root and Isatis indigotica. However, Isatis indigotica has limited salt-alkali tolerance. It can generally grow normally in mild saline-alkali land, but it is difficult to grow normally in saline-alkali soils with a saline content of more than 0.6%. Therefore, developing an efficient and high-quality cultivation method for Isatis indigotica on severe saline-alkali land is of great significance for obtaining high-quality and high-yield Isatis root and Isatis indigotica. Summary of the invention

[0004] Based on the above content, the present invention provides a method for planting Folium Isatidis in severe saline-alkali land.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a method for planting Folium Isatidis in severe saline-alkali land, comprising the following steps:

[0007] (1) Land preparation: Heavy saline-alkali land is mechanically leveled, stones are removed and the land is raked flat, deep plowing is performed to 20-30 cm (the purpose of deep plowing is to break the heavy layer of saline-alkali soil to facilitate the growth of crop roots), and then raked flat;

[0008] (2) Applying basal fertilizer: spreading 10-20 kg / mu of humic acid, 1-5 kg / mu of biochar, 2000-4000 kg / mu of microbial organic fertilizer, 100-150 kg / mu of spent mushroom sticks and 10-15 kg of diammonium phosphate on the soil surface, and deep plowing 25-30 cm, the number of deep plowing is 2 times; wherein, if the amount of humic acid is less than 10 kg / mu, it will not work, and if it is greater than 20 kg / mu, the cost will increase, and it is easy to cause the compaction of the soil to increase, affecting the circulation of air and water, and further affecting the normal growth of plant roots; if the amount of microbial organic fertilizer is less than 2000 kg / mu, the organic fertilizer effect will be insufficient, and if it is greater than 4000 kg / mu, the cost will increase; within the range described in the present invention, humic acid, biochar, microbial organic fertilizer and spent mushroom sticks can play the best synergistic role.

[0009] (3) Ridge sowing: Soak the Radix Isatidis seeds in 40-50°C warm water for 3.5-4.5 hours, remove them and mix them with plant ash, and sow them in late April to early May. Ridge sowing is adopted during sowing, and drip irrigation facilities are laid at the same time; double rows are sown along the length of the ridge platform, with a row spacing of 20 cm, and a 1.5 cm shallow furrow is opened in each row. The Radix Isatidis seeds are evenly scattered in the furrow, covered with 1 cm of soil, pressed, and watered thoroughly with drip irrigation;

[0010] (4) Field management: When the seedlings reach 6-8 cm in height, the seedlings are transplanted with a spacing of 7 cm. In the area where the seedlings are missing, the seedlings are transplanted with soil nearby for supplementary seedlings. One week after the seedlings are supplemented, the soil acid-base regulator is applied through drip irrigation. One week later, the soil acid-base regulator is applied again through drip irrigation. In the middle period, 5 kg / mu of calcium ammonium nitrate and 20-30 kg / mu of compound fertilizer are added. The soil is tilled 4-5 times during the whole growth period. Before harvesting, foliar fertilizer is sprayed once, and the foliar fertilizer consists of 0.1-0.2% zinc fertilizer and 0.1-0.2% potassium fertilizer.

[0011] (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots or spray at the early stage of the disease, or spray with fungicides with equivalent efficacy and low toxicity (for example, spray with 1:1:150 Bordeaux mixture or 65% mancozeb 600-800 times solution at the early stage of downy mildew; spray with 50% carbendazim 1000 times solution at the early stage of root rot); for powdery mildew, spray 30% hexachlorvinphos before the disease or at the early stage of discovery, three times, with an interval of 7-10 days between each spray, or spray with fungicides with equivalent efficacy and low toxicity (for example, 70% thiophanate-methyl wettable powder 1000 times solution);

[0012] (6) Harvest.

[0013] The microbial organic fertilizer is an organic fertilizer containing multiple beneficial microorganisms such as Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum or yeast.

[0014] In a preferred embodiment of the present invention, the salt content of the severe saline-alkali land is 0.6-2.0 wt%.

[0015] In a preferred embodiment of the present invention, in step (2), the ridge platform is 30 cm high, 30 cm wide, and the ridge spacing is 35-40 cm.

[0016] In a preferred embodiment of the present invention, in step (4), the soil acid-base regulator is 25% polyglutamic acid powder; the application amount of the soil acid-base regulator each time is 500-600 g / mu.

[0017] In a preferred embodiment of the present invention, the mid-stage refers to when the seedlings grow to a height of 10-15 cm.

[0018] In a preferred embodiment of the present invention, in step (6), the first harvest is carried out 40 days after emergence, followed by harvests every 25 days, for a total of 5 harvests.

[0019] The present invention also provides a method for increasing the indirubin content in Folium Isatidis, which adopts the Folium Isatidis planting method in heavy saline-alkali land.

[0020] The present invention discloses the following technical effects:

[0021] The invention improves the survival rate and yield of Folium Isatidis in severe saline-alkali land by reasonably applying basal fertilizer and topdressing fertilizer, especially improves the content of effective ingredients such as indigo carmine, so that severe saline-alkali land can be utilized while obtaining higher economic benefits. DETAILED DESCRIPTION

[0022] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0023] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0024] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0025] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0026] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0027] The "%" described in the present invention, unless otherwise specified, refers to mass percentage.

[0028] Due to the high salt and alkalinity of severe saline-alkali land, the growth of traditional Chinese medicinal plants is severely restricted and it is almost difficult for them to survive normally. In response to this problem, the present invention proposes a systematic technical solution for planting Isatis indigofera in severe saline-alkali land, aiming to give full play to the potential of saline-alkali land and improve the yield and quality of Isatis indigofera. The method of the present invention first emphasizes the importance of improving severe saline-alkali soil. Before planting, the soil uses humic acid, biochar, microbial organic fertilizer, and mushroom waste sticks that can inhibit salt and alkali and improve fertility to improve the adaptability of Isatis indigofera to the soil and create a good growth environment for the growth of Isatis indigofera. Then, ridge planting is adopted in combination with drip irrigation to ensure that Isatis indigofera seeds germinate in severe saline-alkali soil and improve the germination rate. At the same time, elaborate post-sowing management measures are formulated, including the use of acid-base balance solution, control of watering procedures, precise application of special fertilizers, weeding and pest control, etc., to ensure that Isatis indigofera is planted in severe saline-alkali soil to achieve high yield.

[0029] The present invention provides a method for planting Folium Isatidis in severe saline-alkali land, comprising the following steps:

[0030] (1) Land preparation: Select a sunny, used or unused coastal saline-alkali land with a content of 0.6-2.0%, mechanically level the land, remove stones and harrow the land, deep plow 20-30 cm and harrow it;

[0031] (2) Applying basal fertilizer: Spread humic acid 10-20 kg / mu, biochar 1-5 kg / mu, microbial organic fertilizer 2000-4000 kg / mu, spent mushroom sticks 100-150 kg / mu, diammonium phosphate 10-15 kg on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0032] (3) Ridge sowing: Soak the seeds in 40-50℃ warm water for about 4 hours, then remove them and mix them with plant ash. Sow them in late April to early May. Ridge sowing is adopted when sowing. The ridge platform is 30 cm high, 30 cm wide, and the ridge spacing is 35-40 cm. At the same time, drip irrigation facilities are laid. Double rows are sown along the length of the ridge platform, with a row spacing of 20 cm. A 1.5 cm shallow trench is opened in each row. The seeds are evenly scattered in the trench, covered with soil about 1 cm, slightly pressed, and watered thoroughly with drip irrigation. Seedlings usually emerge in 5-7 days;

[0033] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for thinning. In areas where seedlings are missing, transplant the seedlings with soil nearby for supplementary seedlings. Apply soil acid-base regulators through drip irrigation one week after the seedlings are supplemented, with an application rate of 10-40g / mu. Continue drip irrigation once after one week. In the mid-term, add 5kg / mu of calcium ammonium nitrate and 20-30kg / mu of compound fertilizer. Till the soil 4-5 times during the entire growth period; spray foliar fertilizer once before harvesting. The foliar fertilizer consists of 0.1-0.2% zinc fertilizer and 0.1-0.2% potassium fertilizer;

[0034] (5) Pest and disease control: For downy mildew and root rot, use 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride at the early stage of the disease, apply to the roots or spray for control, or spray with a fungicide with equivalent efficacy and low toxicity; for powdery mildew, use 30% hexachlorvinphos before the disease or at the early stage of discovery, spray three times with an interval of 7-10 days, or spray with a pesticide with equivalent efficacy and low toxicity; the present invention does not impose any particular limitation on the amount of drugs used for pest and disease control, and the conventional amount used for pest and disease control in this field can be used.

[0035] (6) Harvesting: The first harvest is 40 days after emergence, followed by five harvests every 25 days.

[0036] The zinc fertilizer is zinc sulfate; the potassium fertilizer is potassium dihydrogen phosphate.

[0037] The method of the invention significantly improves the germination rate, growth rate, yield and medicinal component content of Folium Isatidis by improving the soil environment, implementing modern agronomic measures, optimizing the planting procedure and management, and applying fertilizers scientifically.

[0038] The technical solutions described in the present invention, unless otherwise specified, are all conventional solutions in the art, and the reagents or raw materials used, unless otherwise specified, are purchased from commercial channels or have been disclosed.

[0039] The microbial organic fertilizer used in the embodiment of the present invention was purchased from Hebei Sanfeng Biological Fertilizer Co., Ltd., and the main parameters were that the organic matter content was greater than 45%, and the effective live bacteria count of Bacillus subtilis was 20 million / g.

[0040] The compound fertilizer used in the examples of the present invention was purchased from Jiangsu Huachang Chemical Industry Co., Ltd., N:P2O5:K2O=15:15:15.

[0041] The spent mushroom sticks used in the embodiments of the present invention were purchased from Pingquan County, Chengde City, Hebei Province, with a moisture content of 17.3%, cellulose of 27.5%, lignin of 11.6%, and ash of 14.1%.

[0042] The 25% polyglutamic acid powder used in the examples of the present invention was purchased from Shandong Wuyang Biotechnology Co., Ltd. and contained 25% polyglutamic acid.

[0043] The unused coastal saline-alkali wasteland in the embodiment of the present invention is a severe saline-alkali land, 5-15 km away from the sea, with a salt content of 0.6-2.0%, a pH value of 8.1-8.7, an organic matter of 6.3-9.7 g / kg, and a cation exchange capacity of 10.5-11.7 cmol / kg.

[0044] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0045] Example 1

[0046] A method for planting Folium Isatidis in severe saline-alkali land comprises the following steps:

[0047] (1) Land preparation: In unused coastal saline-alkali wasteland with an average salt content of 2.0%, the land is leveled mechanically, stones are removed and the land is raked flat, deep plowing 20-30 cm and raking flat;

[0048] (2) Applying basal fertilizer: Spread 20 kg / mu of humic acid, 1 kg / mu of biochar, 4000 kg / mu of microbial organic fertilizer, 150 kg / mu of spent mushroom sticks, and 10 kg / mu of diammonium phosphate on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0049] (3) Ridge sowing: Select one-year-old Isatis indigo seeds (i.e. Isatis seeds) that are large, full, not shrunken or poisonous, and have a high germination rate. Soak the seeds in 40-50°C warm water for 4 hours, then remove and mix with wood ash. Sow in early May. Use ridge sowing, with a ridge platform of 30 cm high, 30 cm wide, and 35-40 cm between ridges. Lay drip irrigation facilities at the same time. Plant in double rows along the back of the ridge, with a row spacing of 20 cm. Open a 1.5 cm shallow trench in each row, evenly spread the seeds in the trench, cover with soil about 1 cm, slightly suppress, and water thoroughly with drip irrigation. Seedlings will begin to emerge in 5 days, and the emergence rate will reach 92% after 10 days.

[0050] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for transplanting. In areas where seedlings are missing, transplant the seedlings with soil nearby. Apply 25% polyglutamic acid powder, a soil acid-base regulator, through drip irrigation one week after transplanting, at a rate of 40g / mu. Continue drip irrigation one week later, at a rate of 40g / mu. Add 5kg / mu of calcium ammonium nitrate and 20kg / mu of compound fertilizer in mid-July. When weeds appear during the entire growth period, weed and till the soil for 5 times; spray foliar fertilizer once before harvesting. The foliar fertilizer consists of 0.2% zinc fertilizer and 0.1% potassium fertilizer by mass concentration;

[0051] (5) Disease and pest control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots for control in the early stage of the disease. For powdery mildew, spray 30% hexachlorvinphos at the early stage of discovery, once every 7 days, for a total of three times, using the normal amount.

[0052] (6) Harvesting and quality testing of Folium Isatidis: The first harvest was 40 days after emergence, followed by five harvests every 25 days, with a yield of 942 kg / mu.

[0053] Example 2

[0054] A method for planting Folium Isatidis in severe saline-alkali land comprises the following steps:

[0055] (1) Land preparation: In unused coastal saline-alkali wasteland with an average salt content of 1.6%, the land is leveled mechanically, stones are removed and the land is raked flat, plowed 20-30 cm deep and raked flat;

[0056] (2) Applying basal fertilizer: Spread 15 kg / mu of humic acid, 3 kg / mu of biochar, 3000 kg / mu of microbial organic fertilizer, 100 kg / mu of spent mushroom sticks and 20 kg / mu of diammonium phosphate on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0057] (3) Ridge sowing: Select large, full, non-shrunken and non-toxic Isatis indigotica seeds (i.e. Isatis indigotica seeds) within one year. Soak the seeds in 40-50℃ warm water for about 4 hours, then remove and mix with wood ash. Sow in late April. Ridge sowing is adopted, with a ridge platform of 30 cm high, 30 cm wide, and 35-40 cm between ridges. At the same time, lay drip irrigation facilities; plant in double rows along the back of the ridge, with a row spacing of 20 cm. Open a 1.5 cm shallow trench in each row, evenly spread the seeds in the trench, cover with soil about 1 cm, slightly suppress, and water thoroughly with drip irrigation. Seedlings will begin to emerge in 5 days, and the emergence rate will reach 90% after 10 days.

[0058] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for transplanting. In areas where seedlings are missing, transplant the seedlings with soil nearby. Apply 25% polyglutamic acid powder, a soil acid-base regulator, through drip irrigation one week after transplanting, at a rate of 30g / mu. Continue drip irrigation once after one week, at a rate of 30g / mu. In the mid-term, add 5kg / mu of calcium ammonium nitrate and 20kg / mu of compound fertilizer. When weeds appear during the entire growth period, shovel and weed and cultivate the soil for 5 times; spray foliar fertilizer once before harvesting. The foliar fertilizer consists of 0.1% zinc fertilizer and 0.2% potassium fertilizer by mass concentration;

[0059] (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots for control in the early stage of the disease; for powdery mildew, spray 30% hexachlorvinphos at the early stage of the disease, once every 7 days, for a total of three times;

[0060] (6) Harvesting and quality testing of Folium Isatidis: The first harvest was 40 days after emergence, followed by five harvests every 25 days. The fresh leaf yield reached 988 kg / mu.

[0061] Example 3

[0062] A method for planting Folium Isatidis in severe saline-alkali land comprises the following steps:

[0063] (1) Land preparation: In unused coastal saline-alkali wasteland with an average salt content of 1.6%, the land is leveled mechanically, stones are removed and the land is raked flat, plowed 20-30 cm deep and raked flat;

[0064] (2) Applying basal fertilizer: Spread 20 kg / mu of humic acid, 1 kg / mu of biochar, 2000 kg / mu of microbial organic fertilizer, 150 kg / mu of spent mushroom sticks, and 20 kg / mu of diammonium phosphate on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0065] (3) Ridge sowing: Select large and full-grained, non-shrunken and non-toxic Isatis indigotica seeds (i.e. Isatis indigotica seeds) within one year, with a high germination rate. Soak the seeds in 40-50℃ warm water for about 4 hours, then remove and mix with plant ash, and sow in early May. Ridge sowing is adopted, with a ridge platform of 30 cm high, 30 cm wide, and 35-40 cm between ridges. At the same time, drip irrigation facilities are laid; double-row strip sowing is carried out along the back of the ridge, with a row spacing of 20 cm, and a 1.5 cm shallow furrow is opened in each row. The seeds are evenly scattered in the furrow, covered with soil of about 1 cm, lightly pressed, and watered thoroughly with drip irrigation. Seedlings begin to emerge in 7 days, and the emergence rate reaches 88% after 10 days;

[0066] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for transplanting. In areas where seedlings are missing, transplant the seedlings with soil nearby. Apply a soil acid-base regulator 25% polyglutamic acid powder through drip irrigation one week after transplanting, with an application rate of 20g / mu. Continue drip irrigation once after one week, with an application rate of 30g / mu. In the mid-term, add 5kg / mu of calcium ammonium nitrate and 20kg / mu of compound fertilizer. When weeds appear during the entire growth period, shovel and weed and cultivate the soil for 5 times; spray foliar fertilizer once before harvesting. The foliar fertilizer consists of 0.1-0.2% zinc fertilizer and 0.1-0.2% potassium fertilizer;

[0067] (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots for control in the early stage of the disease; for powdery mildew, spray 30% hexachlorvinphos at the early stage of the disease, once every 7 days, for a total of three times;

[0068] (6) Harvesting and quality testing of Folium Isatidis: The first harvest was 40 days after emergence, followed by five harvests every 25 days. The fresh leaf yield reached 1073 kg / mu.

[0069] Example 4

[0070] A method for planting Folium Isatidis in severe saline-alkali land comprises the following steps:

[0071] (1) Land preparation: In unused coastal saline-alkali wasteland with an average salt content of 1.0%, the land is leveled mechanically, stones are removed and the land is raked flat, plowed 20-30 cm deep and raked flat;

[0072] (2) Application of basal fertilizer: spread 15 kg / mu of humic acid, 5 kg / mu of biochar, 2500 kg / mu of microbial organic fertilizer, 120 kg / mu of spent mushroom sticks and 10 kg / mu of diammonium phosphate on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0073] (3) Ridge sowing: Select large, full, non-shrunken and non-toxic seeds of Isatis indigotica seeds (i.e. Isatis indigotica seeds) within one year with a high germination rate. Soak the seeds in 40-50℃ warm water for about 4 hours, then remove and mix with wood ash. Sow in late April. Use ridge sowing method, with ridge height of 30 cm, ridge width of 30 cm, and ridge spacing of 35-40 cm. Lay drip irrigation facilities at the same time. Plant in double rows along the back of the ridge, with a row spacing of 20 cm. Open a 1.5 cm shallow trench in each row, evenly spread the seeds in the trench, cover with soil about 1 cm, slightly suppress, and water thoroughly with drip irrigation. Seedlings will begin to emerge in 5 days, and the emergence rate will reach 95% after 10 days.

[0074] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for transplanting. In areas where seedlings are missing, transplant the seedlings with soil nearby for supplementary seedlings. Apply 25% polyglutamic acid powder by drip irrigation one week after the seedlings are transplanted, with an application rate of 25g / mu. Continue drip irrigation once after one week, with an application rate of 25g / mu. In the mid-term, add 5kg / mu of calcium ammonium nitrate and 20kg / mu of compound fertilizer. When weeds appear during the entire growth period, shovel and weed and cultivate the soil for 5 times; spray foliar fertilizer once before harvesting. The foliar fertilizer consists of 0.2% zinc fertilizer and 0.1% potassium fertilizer by mass concentration;

[0075] (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots for control in the early stage of the disease; for powdery mildew, spray 30% hexachlorvinphos at the early stage of the disease, once every 7 days, for a total of three times;

[0076] (6) Harvesting and quality testing of Isatis indigofera: The first harvest was 40 days after emergence, followed by five harvests every 25 days. The fresh leaf yield reached 1125 kg / mu.

[0077] Example 5

[0078] A method for planting Folium Isatidis in severe saline-alkali land comprises the following steps:

[0079] (1) Land preparation: In unused coastal saline-alkali wasteland with an average salt content of 0.6%, the land is leveled mechanically, stones are removed and the land is raked flat, plowed 20-30 cm deep and raked flat;

[0080] (2) Applying basal fertilizer: Spread 15 kg / mu of humic acid, 1 kg / mu of biochar, 2000 kg / mu of microbial organic fertilizer, 150 kg / mu of spent mushroom sticks, and 10 kg / mu of diammonium phosphate on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0081] (3) Ridge sowing: Select large, full, non-shrunken and non-toxic Isatis indigotica seeds (i.e. Isatis indigotica seeds) within one year. Soak the seeds in 40-50℃ warm water for about 4 hours, then remove and mix with plant ash. Sow at the end of April. Ridge sowing is adopted, with a ridge platform of 30 cm high, 30 cm wide, and 35-40 cm between ridges. At the same time, drip irrigation facilities are laid; double-row strip sowing is carried out along the back of the ridge, with a row spacing of 20 cm. A 1.5 cm shallow furrow is opened in each row. The seeds are evenly scattered in the furrow, covered with soil of about 1 cm, lightly pressed, and watered thoroughly with drip irrigation. Seedlings begin to emerge in 5 days, and the emergence rate reaches 98% after 10 days.

[0082] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for transplanting. In areas where seedlings are missing, transplant the seedlings with soil nearby. Apply 25% polyglutamic acid powder by drip irrigation one week after transplanting, with an application rate of 35g / mu. Continue drip irrigation once after one week, with an application rate of 10g / mu. In the mid-term, add 5kg / mu of calcium ammonium nitrate and 15kg / mu of compound fertilizer. When weeds appear during the entire growth period, shovel and weed and cultivate the soil for 5 times; spray foliar fertilizer once before harvesting, which consists of 0.1% zinc fertilizer and 0.1% potassium fertilizer;

[0083] (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots for control in the early stage of the disease; for powdery mildew, spray 30% hexachlorpyrifos at the early stage of the disease, once every 7 days, for a total of three times;

[0084] (6) Harvesting and quality testing of Isatis indigofera: The first harvest was 40 days after emergence, followed by five harvests every 25 days. The fresh leaf yield reached 1133 kg / mu.

[0085] Example 6

[0086] A method for planting Folium Isatidis in severe saline-alkali land comprises the following steps:

[0087] (1) Land preparation: In unused coastal saline-alkali wasteland with an average salt content of 0.8%, the land is leveled mechanically, stones are removed and the land is raked flat, plowed 20-30 cm deep and raked flat;

[0088] (2) Applying basal fertilizer: Spread 10 kg / mu of humic acid, 4 kg / mu of biochar, 2200 kg / mu of microbial organic fertilizer, 110 kg / mu of spent mushroom sticks, and 10 kg / mu of diammonium phosphate on the soil surface, and deep plow 25-30 cm, twice to ensure that it is evenly mixed with the soil;

[0089] (3) Ridge sowing: Select large, full, non-shrunken and non-toxic Isatis indigotica seeds (i.e. Isatis indigotica seeds) within one year. Soak the seeds in 40-50℃ warm water for about 4 hours, then remove and mix with wood ash. Sow in early May. Ridge sowing is adopted, with a ridge platform of 30 cm high, 30 cm wide, and 35-40 cm between ridges. At the same time, lay drip irrigation facilities; plant in double rows along the back of the ridge, with a row spacing of 20 cm, and open a 1.5 cm shallow trench for each row. Evenly spread the seeds in the trench, cover with soil about 1 cm, and press lightly. Water thoroughly with drip irrigation. Seedlings begin to emerge in 5 days, and the emergence rate reaches 96% after 10 days.

[0090] (4) Field management: When the seedlings reach 6-8cm in height, use a plant spacing of 7cm for transplanting. In areas where seedlings are missing, transplant the seedlings with soil nearby. Apply 25% polyglutamic acid powder by drip irrigation one week after transplanting, with an application rate of 15g / mu. Continue drip irrigation once after one week, with an application rate of 40g / mu. In the mid-term, add 5kg / mu of calcium ammonium nitrate and 20kg / mu of compound fertilizer; when weeds appear during the whole growth period, shovel and weed and cultivate the soil 5 times; spray foliar fertilizer once before harvesting, and the foliar fertilizer consists of 0.15% zinc fertilizer and 0.15% potassium fertilizer by mass concentration;

[0091] (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots for control in the early stage of the disease; for powdery mildew, spray 30% hexachlorpyrifos at the early stage of the disease, once every 7 days, for a total of three times;

[0092] (6) Harvesting and quality testing of Isatis indigofera: The first harvest was 40 days after emergence, followed by five harvests every 25 days. The fresh leaf yield reached 1107 kg / mu.

[0093] The test method for counting the content of indigo carmine, the main active ingredient in each example, adopts liquid chromatography, referring to Parts 1 and 4 of the 2020 edition of the Pharmacopoeia of the People's Republic of China.

[0094] The germination rate (%) 10 days after sowing, the fresh leaf yield of Folium Isatidis (kg / mu), and the indigo carmine content (%) in fresh leaves in Examples 1-6 are statistically shown in Table 1.

[0095] Table 1 Germination rate, fresh leaf yield and indigo content of Folium Isatidis

[0096]

[0097] It can be seen from Table 1 that the indigo carmine content of Isatis indigotica produced in Examples 1-6 of the present invention is higher than 0.02% specified in the 2020 "Pharmacopoeia of the People's Republic of China", and the content is stable throughout the planting process.

[0098] Except for the scheme recorded in this application, other field management such as weeding, tillage management, pest and disease control during the cultivation of Folium Isatidis not recorded in the present invention is carried out according to the existing technology.

[0099] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for planting Folium Isatidis in severe saline-alkali land, characterized in that: The following steps are involved: (1) Land preparation: Heavy saline-alkali land is mechanically leveled, stones are removed and the land is raked flat, deep plowing 20-30 cm and raking flat; (2) Application of basal fertilizer: spread humic acid 10-20 kg / mu, biochar 1-5 kg / mu, microbial organic fertilizer 2000-4000 kg / mu, spent mushroom sticks 100-150 kg / mu and diammonium phosphate 10-15 kg on the soil surface, and deep plow 25-30 cm, twice; (3) Ridge sowing: Soak the Radix Isatidis seeds in 40-50°C warm water for 3.5-4.5 hours, remove them and mix them with plant ash, and sow them in late April to early May. Ridge sowing is adopted during sowing, and drip irrigation facilities are laid at the same time; double rows are sown along the length of the ridge platform, with a row spacing of 20-25 cm, and a 1.5 cm shallow furrow is opened in each row. The Radix Isatidis seeds are evenly scattered in the furrow, covered with 1 cm of soil, pressed, and watered thoroughly with drip irrigation; (4) Field management: When the seedlings reach 6-8 cm in height, the seedlings are transplanted with a spacing of 7 cm; the seedlings are transplanted with soil in the area where the seedlings are missing. A soil acid-base regulator is applied by drip irrigation one week after the seedlings are transplanted, and a soil acid-base regulator is applied again by drip irrigation one week later; 5 kg / mu of calcium ammonium nitrate and 20-30 kg / mu of compound fertilizer are added in the middle period; the soil is tilled 4-5 times during the whole growth period; foliar fertilizer is sprayed once before harvesting, and the foliar fertilizer consists of 0.1-0.2% zinc fertilizer and 0.1-0.2% potassium fertilizer; (5) Pest and disease control: For downy mildew and root rot, apply 62.5 g / L fluopyram and 625 g / L cymoxanil hydrochloride to the roots or spray for prevention and control at the early stage of the disease, or spray with fungicides with equivalent efficacy and low toxicity; for powdery mildew, spray 30% hexachlorpyrifos before the disease or at the early stage of discovery, three times with an interval of 7-10 days between each spray, or spray with fungicides with equivalent efficacy and low toxicity; (6) Harvest.

2. The method for planting Folium Isatidis in severe saline-alkali land according to claim 1, characterized in that: The salt content of the severe saline-alkali land is 0.6-2.0wt%.

3. The method for planting Folium Isatidis in severe saline-alkali land according to claim 1, characterized in that: In step (3), the ridge platform is 30 cm high, 30 cm wide, and the ridge spacing is 35-40 cm.

4. The method for planting Folium Isatidis in severe saline-alkali land according to claim 1, characterized in that: In step (4), the soil acid-base regulator is 25% polyglutamic acid powder or wood vinegar; the application amount of the soil acid-base regulator is 500-600g / mu each time.

5. The method for planting Folium Isatidis in severe saline-alkali land according to claim 1, characterized in that: The mid-stage refers to when the seedlings grow to 10-15 cm in height.

6. The method for planting Folium Isatidis in severe saline-alkali land according to claim 1, characterized in that: In step (6), the first harvest is carried out 40 days after seedling emergence, and then harvested every 25 days, for a total of 5 harvests.

7. A method for increasing the indirubin content in Folium Isatidis, characterized in that: The method for planting Folium Isatidis in severe saline-alkali land according to any one of claims 1 to 6 is adopted.

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