Application of Dioscorea composita alcohol extract in preparation of antibacterial product and antibacterial hand sanitizer

Abstract

The invention discloses an application of a Dioscorea composita alcohol extract in preparation of an antibacterial product and an antibacterial hand sanitizer. The research finds that the Dioscorea composita alcohol extract has a good antibacterial effect, so the application of the Dioscorea composita alcohol extract in preparation of antibacterial products is provided. On the basis, the extraction process of the Dioscorea composita alcohol extract is optimized, and the antibacterial effect of the Dioscorea composita alcohol extract is improved. Besides, the problem that the Dioscorea composita alcohol extract has an unpleasant traditional Chinese medicine smell is further solved through formula optimization, the developed hand sanitizer has excellent comprehensive properties such as smell, skin feeling, color and washing degree, the bacteriostatic effect on escherichia coli and staphylococcus aureus reaches 99% or above, and the hand sanitizer is high in foaming performance and decontamination ability, high in safety and free of toxic and side effects. The heat resistance and cold resistance meet relevant standards of the hand sanitizer. The application range of the dioscorea composita is widened, a new natural bacteriostatic component choice is provided for bacteriostatic products, and the dioscorea composita extract has important value and popularization and application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of daily necessities, and particularly relates to application of an alcohol extract of Dioscorea zingiberensis in preparing an antibacterial product and an antibacterial hand soap. Background Art

[0002] Cleaning of hands and other skin hygiene is not only an important means for hospitals to prevent and control viral infections, but also the main way to prevent bacterial infection in people's daily lives. Therefore, daily cleaning and washing products with antibacterial effects, such as antibacterial hand soaps, antibacterial shower gels, antibacterial laundry detergents for underwear, etc., are favored by more and more consumers; especially antibacterial hand soaps have gradually become an indispensable part of people's daily lives. At present, commercially available antibacterial hand soaps mainly achieve antibacterial functions by adding chemical antibacterial agents, but such additives not only harm the ecological environment, but also have certain damage to the skin of the hands, and consumers may feel uncomfortable when using them. Therefore, antibacterial hand soaps that use natural plant products as effective antibacterial ingredients have attracted much attention due to their mild characteristics.

[0003] Dioscorea composita Hemsl. is native to Mexico and has been introduced and cultivated in my country for more than 30 years. Current research has found that Dioscorea composita Hemsl. contains polysaccharides, amino acids, fatty acids, dioscin compounds, trace elements, starch, protein and other ingredients. It also contains rich active ingredients such as steroidal saponins, triterpenoid saponins, flavonoids, phenanthrene and bibenzyl. Among them, steroidal saponins have surface active properties, can significantly reduce the surface tension of water, and easily form persistent foam in aqueous solution. In addition, some studies have reported that some saponins also have antibacterial activity, so Dioscorea composita Hemsl. extract is expected to become an ideal ingredient for manufacturing antibacterial cleaning and washing products.

[0004] However, the biggest problem with using natural products is that the ingredients of natural plant extracts are complex, the content of specific ingredients is low, and the efficacy is difficult to control. The ingredients of Dioscorea zingiberensis extracts are complex and diverse, and it is not very clear which specific types of saponins have good antibacterial effects. There is still a lot of uncertainty in the development of antibacterial cleaning products using Dioscorea zingiberensis extracts. In addition, Dioscorea zingiberensis has a relatively unique Chinese medicine flavor, which will cause the product to have a more unpleasant odor. If you want to make a hand sanitizer product for daily use, solving the unpleasant odor is also a big problem. Summary of the invention

[0005] The present invention aims to overcome the above problems. Through the study of the effective components of the tubers of Dioscorea zingiberensis, it is proposed that the alcohol extract of Dioscorea zingiberensis has a good antibacterial effect, and further explores the extraction method and extraction conditions with the best antibacterial effect. The problem of the unpleasant taste of Chinese medicine in the extract of Dioscorea zingiberensis is solved through formula optimization. A hand sanitizer formula using the alcohol extract of Dioscorea zingiberensis as an antibacterial additive is developed. The antibacterial effect on Escherichia coli and Staphylococcus aureus can reach more than 99%, the foaming performance and decontamination ability are strong, and the heat and cold resistance meet the relevant standards of hand sanitizer. Moreover, the hand sanitizer has excellent comprehensive performance such as smell, skin feel, color, and flushing degree.

[0006] The first object of the present invention is to provide the use of the ethanol extract of Dioscorea zingiberensis in preparing antibacterial products.

[0007] The third object of the present invention is to provide an antibacterial product.

[0008] The fourth object of the present invention is to provide an antibacterial hand soap.

[0009] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0010] The invention proposes that the alcohol extract of Dioscorea zingiberensis has a good antibacterial effect by studying the effective components of the tubers of Dioscorea zingiberensis, and further explores the best extraction method and extraction conditions for the antibacterial effect, and the obtained extract has a very good inhibitory effect on Escherichia coli and Staphylococcus aureus. The problem that the alcohol extract of Dioscorea zingiberensis has an unpleasant taste of traditional Chinese medicine is further solved by formula optimization. In production and application, the alcohol extract of Dioscorea zingiberensis can be used as an antibacterial additive to prepare daily chemical cleaning and washing products, such as antibacterial hand soap, antibacterial shower gel, antibacterial laundry detergent for underwear, etc.

[0011] Firstly, the present invention seeks to protect the use of the ethanol extract of Dioscorea zingiberensis in the preparation of antibacterial products.

[0012] Preferably, the Dioscorea zingiberensis ethanol extract is Dioscorea zingiberensis ethanol extract.

[0013] More preferably, the mass volume ratio of Dioscorea zingiberensis to ethanol is 1:(10-20).

[0014] More preferably, the mass volume ratio of Dioscorea zingiberensis to ethanol is 1:15-20.

[0015] Most preferably, the mass volume ratio of Dioscorea zingiberensis to ethanol is 1:20.

[0016] Specifically, the mass fraction of the ethanol used is preferably 70% to 80%.

[0017] More preferably, the concentration of ethanol is 70%.

[0018] Further preferably, the Dioscorea zingiberensis ethanol extract is obtained by using cell disruption technology to assist ethanol extraction.

[0019] Preferably, the cell disruption technique is sonication.

[0020] Preferably, the ultrasonic treatment time is 20 to 40 minutes.

[0021] More preferably, the ultrasonic treatment time is 30 to 40 minutes.

[0022] Most preferably, the ultrasonic treatment time is 40 min.

[0023] Preferably, the power of the ultrasonic treatment is 500-700W.

[0024] More preferably, the power of the ultrasonic treatment is 600W.

[0025] Specifically, the preferred extraction scheme of the Dioscorea zingiberensis alcohol extract is: adding 70% to 80% ethanol to the Dioscorea zingiberensis tuber powder at a solid-liquid ratio of 1: (10 to 20), ultrasonically treating at 500 to 700W for 20 to 40 minutes, and then extracting to obtain the Dioscorea zingiberensis tuber extract.

[0026] Specifically and preferably, the extraction is carried out at a constant temperature of 35-45° C. and a uniform speed of 150-300 rpm.

[0027] More preferably, the extraction is carried out at a constant temperature of 37° C. and a constant speed of 200 rpm.

[0028] More preferably, the extraction time is 20 h to 36 h.

[0029] Most preferably, the extraction time is 24 hours.

[0030] The present invention also claims protection for an antibacterial product using the alcohol extract of Dioscorea zingiberensis as an antibacterial component. Specifically and preferably, the content of the alcohol extract of Dioscorea zingiberensis in the antibacterial product is 1% to 12% by mass.

[0031] Preferably, the extraction method and conditions of the Dioscorea zingiberensis ethanol extract are as described above.

[0032] The ethanol extract of Dioscorea zingiberensis obtained by the above method can be concentrated and then used as an additive to prepare an antibacterial product. Specifically, it can be concentrated to 40% to 60% of the original volume, preferably to 50% of the original volume.

[0033] More preferably, the content of the Dioscorea zingiberensis ethanol extract in the antibacterial product is 2% to 8% by mass ratio.

[0034] More preferably, the content of the Dioscorea zingiberensis ethanol extract in the antibacterial product is 3-6% by mass ratio.

[0035] Specifically, the antibacterial products include but are not limited to: antibacterial hand soap, antibacterial shower gel, antibacterial laundry detergent for underwear, etc.

[0036] As an optional antibacterial product solution, the present invention claims protection for an antibacterial hand sanitizer.

[0037] Specifically, the formula of the antibacterial hand sanitizer includes, by mass percentage: 1% to 12% of Dioscorea zingiberensis alcohol extract, 8% to 12% of sodium polyoxyethylene fatty alcohol ether sulfate (AES), 0.75% to 1.25% of glycerol, 1% to 4% of coconut oil diethanolamide, 1% to 3% of thickener, pH adjuster to adjust the pH to 6.5 to 7.5, and the balance is water.

[0038] Preferably, the content of the Dioscorea zingiberensis ethanol extract is 2% to 8%.

[0039] More preferably, the content of the Dioscorea zingiberensis ethanol extract is 3% to 6%.

[0040] As a specific implementation, the Dioscorea zingiberensis ethanol extract can be extracted according to the method described above, and the obtained Dioscorea zingiberensis ethanol extract can be concentrated before use, specifically, it can be concentrated to 40% to 60% of the original volume, preferably to 50% of the original volume.

[0041] When the concentrated chrysanthemum leaf yam alcohol extract is added to the hand sanitizer formula, the addition amount is preferably 1% to 6%, more preferably 2% to 4%, and most preferably 3%.

[0042] In addition, preferably, the content of AES is 8% to 10%.

[0043] More preferably, the content of AES is 9%.

[0044] Preferably, the content of glycerol is 0.8% to 1.2%.

[0045] More preferably, the content of glycerol is 1%.

[0046] Preferably, the content of coconut oil diethanolamide is 2% to 3%.

[0047] More preferably, the content of coconut oil diethanolamide is 2.5%.

[0048] Preferably, the content of the thickener is 2%.

[0049] Preferably, the thickener is at least one of sodium chloride and potassium chloride.

[0050] More preferably, the thickener is sodium chloride.

[0051] Specifically, the pH regulator includes one or more of tartaric acid, citric acid, and malic acid.

[0052] More specifically, the pH regulator is citric acid.

[0053] Preferably, the pH adjuster adjusts the pH to 7.0.

[0054] The present invention has the following beneficial effects:

[0055] The present invention has found that the alcohol extract of Dioscorea zingiberensis has a good antibacterial effect, and therefore has good application value in developing antibacterial products such as antibacterial hand soap, antibacterial shower gel, antibacterial laundry detergent for underwear and the like.

[0056] The present invention further explores the best extraction method and extraction conditions for antibacterial effect by studying the extraction process of effective ingredients of Dioscorea zingiberensis tubers. That is, Dioscorea zingiberensis tuber powder is added to ethanol at a solid-liquid ratio of 1: (10-20), and extraction is performed after ultrasonic treatment for 20-40 minutes. The obtained extract has a very good inhibitory effect on Escherichia coli and Staphylococcus aureus.

[0057] The present invention further solves the problem of the unpleasant Chinese medicinal taste of the chrysanthemum leaf Dioscorea alcohol extract through formula optimization, and develops a hand sanitizer formula using the chrysanthemum leaf Dioscorea alcohol extract as an antibacterial additive. The antibacterial effect on Escherichia coli and Staphylococcus aureus can reach more than 99%, the foaming performance and decontamination effect are strong, and the heat and cold resistance meet the relevant standards of hand sanitizer. The hand sanitizer has excellent comprehensive performance such as smell, skin feel, color, and flushing degree.

[0058] The invention not only broadens the application scope of Dioscorea zingiberensis, but also enriches the sources of effective ingredients that can be used to prepare antibacterial products, and is beneficial to the development of green and natural antibacterial products. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] Figure 1 This is a foaming stability test of hand sanitizers prepared with different AES contents. Examples 1, 2, and 3 in the figure refer to hand sanitizers prepared with AES contents of 8%, 9%, and 10%, respectively. DETAILED DESCRIPTION

[0060] The present invention is further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any form.

[0061] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the present technical field.

[0062] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0063] Example 1 Preparation of Dioscorea zingiberensis tuber extract and its antibacterial activity detection and optimal extraction conditions

[0064] 1. Extraction method of Dioscorea zingiberensis tuber extract

[0065] 1 g of Dioscorea zingiberensis powder was taken and placed in a centrifuge bottle, and 70% anhydrous ethanol was added to make the solid-liquid ratio (mass volume ratio) 1:10, 1:15, and 1:20, respectively. The experimental groups of each solid-liquid ratio were subjected to ultrasonic assisted treatment for 20 min, 30 min, and 40 min, respectively, with an ultrasonic power of 600 W; then the extraction was carried out in a constant temperature shaker at 37 ° C and a constant speed of 200 rpm for 24 h. After the end, the obtained extract was centrifuged and filtered to obtain the Dioscorea zingiberensis alcohol extract, and L9 (3 3 )Orthogonal experiment was used to explore the optimal extraction process.

[0066] 2. Determination of antibacterial activity of ethanol extract of Dioscorea zingiberensis

[0067] (1) Activation of test strains

[0068] Staphylococcus aureus, Escherichia coli MG1655, and Escherichia coli DH5α were taken out from a refrigerator at 4°C, inoculated into fresh LB liquid culture medium, cultured in a constant temperature shaker at 37°C for 24 hours for activation, and placed at 4°C for use.

[0069] Staphylococcus aureus was S. aureus ATCC6538, and Escherichia coli E. coli MG1655 was provided by the Microbiology Laboratory of the College of Life Sciences, South China Agricultural University.

[0070] Escherichia coli DH5α was prepared by our laboratory.

[0071] The composition of LB liquid culture medium is: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, which are dissolved and fixed to volume with water.

[0072] (2) Determination of antibacterial activity by filter paper diffusion method

[0073] Preparation of drug-sensitive paper discs: Cut the filter paper into round discs with a diameter of 6 mm and sterilize them by high pressure for later use.

[0074] Pour the plate: After the prepared LB solid culture medium is sterilized by high pressure and cooled to about 50°C, pour 15 to 20 mL of the culture medium into each culture dish.

[0075] The composition of LB solid culture medium is: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, 15 g / L agar powder, which are dissolved and fixed to volume with water.

[0076] Bacteria: Pipette 200 μL of bacterial solution (OD 600 =0.6~0.7) on the surface of LB solid culture medium, and use a sterile applicator to evenly spread the bacterial solution to ensure that the bacterial solution covers the entire surface of LB solid culture medium.

[0077] Paste the filter paper: Use dry and sterile tweezers to pick up a piece of autoclaved filter paper, stick it on the surface of the LB solid culture medium that has been smeared with the bacteria, press it gently, add 50 μL of Dioscorea zingiberensis ethanol extract on the filter paper, cover the culture dish, and place it in an incubator at 37°C for inversion and culture for 24 hours.

[0078] Measure the diameter of the inhibition zone: Take out the culture dish and measure the diameter of the inhibition zone with a vernier caliper.

[0079] (3) Results analysis

[0080] The results of the determination of the antibacterial activity of the Dioscorea zingiberensis ethanol extracts obtained by different extraction methods against different bacteria are shown in Tables 1, 2, and 3.

[0081] Table 1 is the results of the antibacterial activity test of the ethanol extract of Dioscorea zingiberensis against Escherichia coli DH5α.

[0082] Table 2 is the results of the antibacterial activity test of the ethanol extract of Dioscorea zingiberensis against Escherichia coli MG1655.

[0083] Table 3 is the results of the antibacterial activity test of the ethanol extract of Dioscorea zingiberensis against Staphylococcus aureus.

[0084] The solid-liquid ratio in the table is factor A, the ultrasonic time is factor B, the solid-liquid ratio of 1:10 / 1:15 / 1:20 is the three levels of factor A, namely level A1, A2, and A3, and the ultrasonic time of 20min, 30min, and 40min is the three levels of factor B, namely level B1, B2, and B3. K1 is the average of the sum of the extraction amounts of three tests at level 1 for each factor; K2 is the average of the sum of the extraction amounts of three tests at level 2 for each factor; K3 is the average of the sum of the extraction amounts of three tests at level 3 for each factor; the R value is the range, which indicates the influence of the factor on the result.

[0085] It can be seen from the R values ​​in Tables 1, 2, and 3 that the order of influence of factors A and B is B>A, that is, the ultrasonic time has a greater influence on the antibacterial effect of the Dioscorea zingiberensis alcohol extract than the solid-liquid ratio. According to the size of the K value, for Escherichia coli MG1655 and DH5α, the K3 values ​​in Tables 1 and 2 are greater than the K values ​​at other levels, and the levels of each factor are all at level three, that is, the process A3B3 is the best; for Staphylococcus aureus, the solid-liquid ratio K2 in Table 3 and the ultrasonic time K3 value are the largest, and the process A2B3 is the best. Considering that the ultrasonic time has a greater impact level than the solid-liquid ratio, on the whole, the Dioscorea zingiberensis alcohol extract obtained by the solid-liquid ratio of 1:20 and ultrasonic treatment for 40 minutes has the best inhibitory effect on the three bacteria. Therefore, the Dioscorea zingiberensis alcohol extract obtained under the above conditions is involved in the subsequent process.

[0086] Table 1 Orthogonal test factors, levels and results (Escherichia coli DH5α)

[0087]

[0088]

[0089] Table 2 Orthogonal test factors, levels and results (Escherichia coli MG1655)

[0090]

[0091] Table 3 Orthogonal test factors, levels and results (Staphylococcus aureus)

[0092]

[0093] Example 2 Preparation method of chrysanthemum leaf yam natural antibacterial hand soap

[0094] 1. Concentrated Dioscorea zingiberensis ethanol extract

[0095] The ethanol extract of Dioscorea zingiberensis obtained by the best extraction method in Example 1 was further concentrated by a rotary evaporator to 50% of the original volume before use.

[0096] 2. Formula setting of chrysanthemum leaf and yam natural antibacterial hand sanitizer

[0097] The basic formula of hand sanitizer: by mass ratio, 3% chrysanthemum leaf Dioscorea zingiberensis alcohol extract, 9% sodium polyoxyethylene fatty alcohol ether sulfate (AES), 1% glycerol, 2% sodium chloride, 0.5% citric acid, 2.5% coconut oil diethanolamide, and the rest water.

[0098] Each test was based on the preparation of 100 mL of hand sanitizer. According to the above basic formula, basic single-factor test conditions were set: other basic formulas remained unchanged, and the addition amount of Dioscorea zingiberensis ethanol extract (1%, 3%, 6%), the addition amount of AES (8%, 9%, 12%), and the addition amount of glycerol (0.5%, 1%, 1.25%) were changed respectively.

[0099] The specific preparation method is as follows: based on the preparation of 100mL of hand sanitizer, add 60mL of deionized water into a 200mL beaker, add sodium chloride and coconut oil diethanolamide, heat in a 60°C water bath, and adjust the pH to about 7.0 with citric acid; then add AES and glycerin and stir evenly, finally add the ethanol extract of Dioscorea zingiberensis, add deionized water to make the volume to 100mL, and take out and let it stand for use after it is completely dissolved.

[0100] The effects of three factors, namely, ethanol extract of Dioscorea zingiberensis, AES and glycerol, on the sensory quality of hand sanitizer were investigated. The sensory scoring criteria of hand sanitizer are shown in Table 4.

[0101] Table 4 Sensory scoring criteria for hand sanitizers

[0102] Itemization Excellent (5 points) Good (4 points) Medium (3 points) Bad (2 points) Very bad (1 point) odor fragrance good No odor Unpleasant There is a strange smell Skin feel Wetting Wetter generally Slightly dry dry color bright More transparent Moderate Darker Dark Flushing degree Easy to rinse Easier to rinse Moderate difficulty Difficult to rinse Very difficult to rinse

[0103] 3. Effect of the addition amount of Dioscorea zingiberensis ethanol extract on the performance of hand sanitizer

[0104] Hand sanitizers containing 1%, 3%, and 6% of the alcohol extract of Dioscorea zingiberensis were prepared respectively, and 5 students were randomly selected to evaluate according to the sensory scoring standard of hand sanitizer in Table 4. The sensory evaluation score of each student for each sample was the average of the student's scores for the four items of smell, skin feel, color, and flushing degree of the sample; the total score of each sample was the sum of the scores of the 5 students; the results are shown in Table 5. From the comprehensive analysis of Table 5, it can be seen that when the addition amount of the alcohol extract of Dioscorea zingiberensis is 3%, the hand sanitizer is in the best state, and is significantly better than the addition amounts of 1% and 6% of the alcohol extract.

[0105] Table 5 Sensory evaluation results of ethanol extract of Dioscorea zingiberensis

[0106]

[0107] 4. Effect of glycerol addition on hand sanitizer performance

[0108] Hand sanitizers containing 0.5%, 1%, and 1.25% glycerol were prepared respectively, and 5 students were randomly selected to evaluate according to the sensory scoring standards for hand sanitizers in Table 4. The sensory evaluation score of each student for each sample was the average of the student's scores for the sample's odor, skin feel, color, and flushing degree; the total score of each sample was the sum of the scores of the 5 students; the results are shown in Table 6. As can be seen from Table 6, when the glycerol addition amount is 1%, the sensory score of the hand sanitizer reaches the maximum value, so the glycerol addition amount of 1% is the best, and is significantly better than the glycerol addition amounts of 0.75% and 1.25%.

[0109] Table 6 Sensory scoring results of glycerol content

[0110]

[0111] 5. Effect of AES addition on hand sanitizer performance

[0112] (1) Sensory score measurement

[0113] Hand sanitizers containing 8%, 9%, and 12% AES were prepared respectively, and 5 students were randomly selected to evaluate according to the sensory scoring standards of hand sanitizers in Table 4. The sensory evaluation score of each student for each sample was the average of the student's scores for the sample's odor, skin feel, color, and flushing degree; the total score of each sample was the sum of the scores of the 5 students; the results are shown in Table 7. As can be seen from Table 7, the amount of AES added has a significant effect on the overall sensory score of hand sanitizer. When the amount of AES added is 9%, the sensory score of the hand sanitizer is the highest, and it is significantly better than the 8% and 12% AES additions.

[0114] Table 7 Sensory evaluation results of AES content

[0115]

[0116] (2) Foaming stability test of hand sanitizer

[0117] Take 2g of hand sanitizer containing 8%, 9%, and 12% AES, add them to 98mL of deionized water respectively, place them in a 250mL graduated cylinder, stir at high speed for 1min, read the foam height every 5min to determine the foam stability, and measure for 30min.

[0118] The foaming stability test results of hand sanitizers prepared with different contents of AES are as follows: Figure 1 As shown. Figure 1 It can be seen that the hand sanitizer with an AES content of 9% has the best foaming stability.

[0119] In summary, the best formula for natural antibacterial hand sanitizer from Dioscorea zingiberensis is as follows: based on the preparation of 100mL of hand sanitizer, add 60mL of deionized water, 2g of sodium chloride, and 2.5g of coconut oil diethanolamide into a 200mL beaker, heat in a 60℃ water bath, and adjust the pH to about 7.0 with citric acid; then add 9g of AES and 1g of glycerol and stir evenly, finally add 3g of Dioscorea zingiberensis alcohol extract, add deionized water to make the volume to 100mL, and prepare hand sanitizer.

[0120] Example 3 Performance and stability analysis of natural antibacterial hand soap made from yam leaf

[0121] The hand sanitizer prepared according to the optimal formula of the natural antibacterial hand sanitizer with chrysanthemum leaves and Dioscorea zingiberensis in Example 2 was sample group A, and the antibacterial hand sanitizer of a well-known commercial brand was sample group B.

[0122] 1. Foaming performance test

[0123] The foaming property of antibacterial hand sanitizer was determined using the traditional foam oscillation method. The specific process is as follows: at room temperature, take 1g of hand sanitizers in groups A and B, add them to 49mL of deionized water, place them in a 250mL graduated cylinder, stir at high speed for 1min, and measure the foam height (mm) and the time t required for the foam to reduce to half of its original height. 1 / 2 (s).

[0124] The results of the foaming test of groups A and B are shown in Table 8. The foam height of group A is higher than that of group B, and the time required for the foam of group A to be reduced to half of its original height is also higher than that of group B, indicating that the homemade hand soap of the present invention has better foaming performance.

[0125] Table 8 Foaming performance test of two groups of hand sanitizers A and B

[0126]

[0127] 2. Decontamination ability test

[0128] Measure 2.5 mL of group A and group B hand sanitizer respectively into a beaker, add distilled water to 100 mL, and shake well. Add motor oil dropwise into the beaker and record the volume added when the motor oil no longer dissolves.

[0129] The results show that in the experiment of group A, when the volume of engine oil added was 2.1 mL, the engine oil was no longer soluble in the hand sanitizer, indicating that the hand sanitizer of group A has a strong decontamination ability. In the experiment of group B, when the volume of engine oil added was 1.8 mL, the engine oil was no longer soluble in the hand sanitizer, indicating that the hand sanitizer of group B also has a strong decontamination ability. In contrast, the hand sanitizer prepared by the present invention has a higher decontamination ability than the commercially available hand sanitizer and has a good decontamination ability.

[0130] 3. Determination of antibacterial activity

[0131] The antibacterial activity of hand sanitizer samples A and B was tested respectively. 1 mL of hand sanitizers from groups A and B were added to LB solid medium (100 mL) cooled to about 50°C, mixed thoroughly and poured into a culture dish. The LB solid medium without hand sanitizer was used as the blank group. The activated Escherichia coli (E.coil) and Staphylococcus aureus (S.aureus) were transferred to LB liquid medium and cultured until OD 600 =0.3-0.4, take 200 μL of bacterial solution to plate. Place the culture dish in a constant temperature incubator at 37°C for 24 hours, count the colonies using the plate colony counting method, record the data, and take the average value. Repeat each group of data 3 times.

[0132] The results of the antibacterial activity test of the hand sanitizers of groups A and B are shown in Table 9. As can be seen from Table 9, the antibacterial rate of the self-made antibacterial hand sanitizer of the present invention against Escherichia coli is 99%, which is significantly better than the commercial hand sanitizer; the antibacterial rate against Staphylococcus aureus is 99.20%, which is the same as the commercial hand sanitizer. In summary, the antibacterial performance of the hand sanitizer prepared by the present invention is better than that of the commercial hand sanitizer.

[0133] Table 9 Antibacterial activity test results of hand sanitizers in groups A and B

[0134]

[0135] 4. Heat resistance and cold resistance test

[0136] Refer to the method described in GB / T34855-2017. Place hand sanitizer sample A and commercial hand sanitizer sample B at (40±1)℃ for 48h, take them out and compare with the original samples to see if they are layered, transparent, discolored, and if the product has odor. Repeat 3 times. If all are qualified, it can be judged that its stability meets the requirements. Parallel determination is performed 3 times.

[0137] Refer to the method described in GB / T34855-2017. Place hand sanitizer sample A and commercial hand sanitizer sample B in a refrigerator at (-5±2)℃ for 48h. When they are taken out and restored to room temperature, observe the product and the original sample to compare whether they are turbid or have flocculation. The measurement is repeated three times in parallel.

[0138] Experimental results: After the heat and cold resistance tests, it was observed that the hand sanitizers in group A and group B had no floccules, no stratification, no corruption or taste change, and no color change, indicating that the hand sanitizer prepared by the present invention has good heat and cold resistance stability.

[0139] In summary, the hand sanitizer prepared by the present invention has good antibacterial performance, foaming performance, and decontamination performance, and is superior to commercially available hand sanitizers.

[0140] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. Application of Dioscorea zingiberensis ethanol extract in the preparation of antibacterial products.

2. The application according to claim 1, characterized in that: The Dioscorea zingiberensis alcohol extract is the Dioscorea zingiberensis alcohol extract.

3. The application according to claim 2, characterized in that: The mass volume ratio of the Dioscorea zingiberensis to ethanol is 1:(10-20); wherein the mass fraction of ethanol is 70%-80%.

4. The use according to claim 1 or 2, characterized in that: The ethanol extract of Dioscorea zingiberensis is obtained by using cell disruption technology to assist ethanol extraction.

5. The application according to claim 4, characterized in that: The cell disruption technique is ultrasonic treatment, and the ultrasonic treatment time is 20 to 40 minutes.

6. The use according to claim 5, characterized in that: The yam zingiberensis alcohol extract is prepared by adding 70% to 80% ethanol to yam zingiberensis tuber powder at a solid-liquid ratio of 1: (10 to 20), and then subjecting the mixture to ultrasonic treatment at 500 to 700W for 20 to 40 minutes, thereby obtaining the yam zingiberensis tuber extract.

7. The use according to claim 6, characterized in that: The extraction is carried out at a constant temperature of 35-45°C and a constant speed of 150-300 rpm.

8. The use according to claim 7, characterized in that: The extraction time is 20h to 36h.

9. An antibacterial product, characterized in that: The antibacterial ingredient is the ethanol extract of Dioscorea zingiberensis.

10. An antibacterial hand soap, characterized in that: Calculated by mass percentage, it includes: 1% to 12% of Dioscorea zingiberensis alcohol extract, 8% to 12% of sodium polyoxyethylene fatty alcohol ether sulfate, 0.75% to 1.25% of glycerol, 1% to 4% of coconut oil diethanolamide, 1% to 3% of thickener, pH regulator to adjust the pH to 6.5 to 7.5, and the balance is water.

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