Method for separating and preparing cis-trans isomers in vitamin K1
Through supercritical fluid chromatography separation method, the mixed mobile phase of supercritical carbon dioxide and organic solvents was used to successfully improve the purity of the vitamin K1 trans isomer, solve the problem that the proportion of vitamin K1 cis isomer in the prior art is difficult to control, and achieve efficient and environmentally friendly separation and preparation effect.
Patent Information
- Application Number
- CN202411803815.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-10
- Publication Date
- 2025-03-21
AI Technical Summary
The prior art is difficult to control the proportion of cis isomers in vitamin K1 to less than 21%, and the purity of trans isomers obtained by high performance liquid chromatography separation still needs to be improved.
The crude vitamin K1 product was dissolved in an organic solvent by supercritical fluid chromatography separation method, and a mixture of supercritical carbon dioxide and n-hexane or n-heptane was used as the mobile phase, and silica gel was used as the chromatographic column filler. The cis and trans isomers were collected respectively by isometric or gradient elution.
The purity of the vitamin K1 trans isomer is achieved by 99%, simplifying the post-processing procedure, reducing the use of organic reagents, and improving the green and environmental protection and economic benefits of the separation method.