Medium preparation device

Abstract

The application discloses a culture medium preparation device, which comprises a control panel and a containing vessel. The surface of the containing vessel is provided with a solution volume metering scale. The containing vessel is provided with stirring blades near the lower part. The bottom and the side of the containing vessel are provided with electromagnetic heating elements. The outer sidewall of the containing vessel is provided with a cooling structure. The sidewall of the containing vessel is connected with a support. The support partially protrudes upwardly to the containing vessel. The support is provided with an acid-base reservoir, a pH probe and a thermometer. The bottom of the acid-base reservoir is provided with a rubber-tipped dropper. The pH probe and the thermometer are respectively used for detecting the pH value and the temperature of the solution in the containing vessel. The bottom of the containing vessel is provided with a leakage valve. The control panel is used for controlling the rotation of the stirring blades, controlling the heating of the electromagnetic heating elements and controlling the cooling of the containing vessel by the cooling structure. The culture medium preparation device can assist manual preparation of the culture medium, saves the labor cost and the time cost and improves the production efficiency of the bacterial strain.

Description

Technical Field

[0001] This utility model relates to the field of culture medium preparation technology, and in particular to a culture medium preparation device. Background Technology

[0002] Culture medium refers to a nutrient substrate composed of different nutrients, prepared to support the growth and reproduction of microorganisms, plants, or animals (or tissues). Different microorganisms require different nutrients, and therefore generally need to be formulated.

[0003] In existing technologies, the preparation process of culture medium in shake flasks in the incubator, including operations such as gelatinization, cooling, volume adjustment, and acid-base adjustment, is mostly carried out manually. The continuity of the process depends on the movement of personnel and manual operation, resulting in high time costs, high labor consumption, and low preparation efficiency.

[0004] Based on this, and after analyzing and studying the culture medium preparation process, and in accordance with the actual production needs for saving time and effort, a culture medium preparation device is proposed to assist manual labor in the rapid preparation of culture media. Utility Model Content

[0005] This application provides a culture medium preparation device that can assist manual labor in the rapid preparation of culture media, thereby saving labor and time costs, improving the production efficiency of microorganisms, and ensuring production progress.

[0006] This application provides a culture medium preparation device, including a control panel and a container. The surface of the container is provided with solution volume measurement scales. A stirring blade is provided near the bottom of the container. Electromagnetic heating elements are provided at the bottom and sides of the container. A cooling structure is provided on the outer side wall of the container. A support is connected to the side wall of the container. The support protrudes upward from the container and is provided with an acid-base reservoir, a pH probe, and a thermometer. A dropper is provided at the bottom of the acid-base reservoir for dripping acid or alkali solution into the container. The pH probe and the thermometer are used to detect the pH value and temperature of the solution in the container, respectively. A leakage valve is provided at the bottom of the container. The control panel is used to control the rotation of the stirring blades, to control the heating of the electromagnetic heating element, and to control the cooling structure to cool the container.

[0007] In one possible implementation, the electromagnetic heating element is integrally disposed within the bottom wall and side wall of the container.

[0008] In one possible implementation, the bottom and sides of the container are provided with an electromagnetic heating plate or an electromagnetic heating layer, and the electromagnetic heating element is disposed within the electromagnetic heating plate or the electromagnetic heating layer.

[0009] In one possible implementation, the bracket includes a connecting part and a supporting part. The supporting part is located above the container and is connected to the container through the connecting part. The supporting part includes a first bracket and a second bracket connected in a cross shape. A rotary motor is installed at the center of the first bracket. A stirring shaft is coaxially connected to the bottom of the rotary motor. The stirring blades are evenly distributed on the stirring shaft. The first bracket is provided with a clamp for fixing the acid-base storage container. The pH probe and the thermometer are both connected to the second bracket through a power cord. The connection position is located outside the container. The control panel is located on the second bracket and outside the container.

[0010] In one possible implementation, the cooling structure includes a condensate coil wound around the surface of the container, the condensate coil being connected to a cold source, and a water pump being mounted on the condensate coil, the water pump being electrically connected to the control panel.

[0011] In one possible implementation, the container is a stainless steel cylindrical container with an arc-shaped bottom, and the leakage valve is installed at the bottom end of the arc-shaped bottom.

[0012] In one possible implementation, the leakage valve is electrically connected to the control panel, which controls the leakage amount and interval of the leakage valve. The bottom of the container is provided with multiple legs to support the container at a predetermined height. The culture medium preparation device also includes a conveyor belt that can stop at intervals. The surface of the conveyor belt is evenly spaced with triangular bottle marking points for placing triangular bottles at designated points. The leakage interval of the leakage valve is matched with the conveying speed of the conveyor belt and the interval distance of the triangular bottle marking points.

[0013] Beneficial effects: Compared with the prior art, the culture medium preparation device provided in this application can automatically heat and gelatinize the materials in the container through stirring blades and electromagnetic heating elements. After gelatinization, the material is automatically cooled by a cooling structure. Then, the remaining materials that do not need to be gelatinized are added to the container and mixed evenly, and the volume is adjusted to the preparation volume. After adjusting the volume, the pH value of the solution is detected by a pH probe, and acid or alkali solution is added in appropriate amounts according to process requirements. This can assist manual labor in quickly completing the preparation of the culture medium. After preparation, the culture medium can be dispensed through a leakage valve, which can save labor and time costs, improve the production efficiency of the strain, and ensure the production schedule.

[0014] These and other objects, features and advantages of this utility model will be fully realized through the following detailed description. Attached Figure Description

[0015] Figure 1 A schematic front view of the culture medium preparation device of this application is shown, in which the rotary motor is not shown.

[0016] Figure 2 A top view of the culture medium preparation apparatus of this application is shown. Detailed Implementation

[0017] The following description is intended to disclose the present invention so that those skilled in the art can implement it. The preferred embodiments described below are merely examples, and other obvious variations will occur to those skilled in the art. The basic principles of the present invention defined in the following description can be applied to other embodiments, modifications, improvements, equivalents, and other technical solutions that do not depart from the spirit and scope of the present invention.

[0018] Those skilled in the art should understand that, in the disclosure of this specification, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the above terms should not be construed as limitations on this utility model.

[0019] It is understood that the term "a" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element can be one, while in another embodiment, the number of the element can be multiple, and the term "a" should not be understood as a limitation on the number.

[0020] refer to Figure 1 and Figure 2This application provides a culture medium preparation device, including a control panel 10 and a container 20. The control panel 10 can also be referred to as a controller with an operation panel. The surface of the container 20 is provided with solution volume measurement scales, which can be used to adjust the volume of the container to achieve a suitable preparation volume of culture medium. A stirring blade 21, or mechanical stirring paddle, is provided near the lower part of the container 20 to stir the solution in the lower part of the container 20. Simultaneously, electromagnetic heating elements, such as electromagnetic heating coils or electromagnetic heaters, are provided at the bottom and sides of the container 20 to heat the solution inside the container. This simultaneous heating and mechanical stirring can accelerate the gelatinization reaction. A cooling structure is provided on the outer wall of the container 20 for rapid cooling. The side wall of the container 20 is connected to a support 30, wherein the support 30 protrudes upward from the container 20, and the support 30 is provided with an acid-base reservoir 23, a pH probe 24 and a thermometer 25. The bottom of the acid-base reservoir 23 is provided with a dropper for dripping an appropriate volume of acid or alkali solution into the container 20. The pH probe 24 and the thermometer 25 are respectively used to detect the pH value and temperature of the solution in the container 20. In addition, the bottom of the container 20 is provided with a leakage valve 26 for dispensing the prepared culture medium. The control panel 10 is used to control the rotation of the stirring blade 21, to control the heating of the electromagnetic heating element, and to control the cooling structure to cool the container 20.

[0021] The work process is roughly as follows: Dissolve the materials to be gelatinized in water and place them in the culture medium container. Turn on the electromagnetic heating element to heat and gelatinize the materials, keeping the stirring blade 21 moving at a low speed during gelatinization. After gelatinization, activate the cooling mechanism to cool the materials. Once the temperature has reached a suitable level, such as by manually touching the mold to confirm that the container 20 has reached room temperature, or by using a thermometer 25 to detect that the temperature of the solution in the container 20 has reached room temperature, add the remaining materials that do not need to be gelatinized into the container and mix them evenly, then bring the volume to the prepared volume. After bringing the volume to the required level, insert the pH probe 24 into the liquid surface and adjust the pH of the culture medium using either acid or alkali, depending on the process requirements. Generally, at a fixed workstation and for a specific culture medium, only acid or only alkali will be used for pH adjustment, not both simultaneously. The acid / alkali storage tank 23 can be either an acid or alkali storage tank. After pH adjustment, set the leakage volume and interval of the leakage valve 26 via the control panel 10 to dispense the culture medium.

[0022] Therefore, the culture medium preparation device provided in this application can assist in the rapid preparation of culture medium by humans, thereby saving labor and time costs, improving the production efficiency of strains, and ensuring production progress.

[0023] In one embodiment, the electromagnetic heating element is integrally disposed within the bottom wall and side wall of the container 20, making the overall structure of the device more compact and saving space.

[0024] In another embodiment, the bottom and sides of the container 20 are provided with an electromagnetic heating plate or an electromagnetic heating layer, and the electromagnetic heating element is located inside the electromagnetic heating plate or the electromagnetic heating layer, thereby facilitating the replacement and maintenance of the electromagnetic heating element.

[0025] In one embodiment, the bracket 30 includes a connecting portion 31 and a supporting portion 32, wherein the supporting portion 32 is located above the holding container 20 and is connected to the holding container 20 through the connecting portion 31. The supporting portion 32 includes a first bracket 321 and a second bracket 322 connected in a cross shape, wherein a rotary motor 33 is installed at the center of the first bracket 321, the rotary motor 33 is directly above the axial center of the holding container 20, and the rotating shaft at the bottom of the rotary motor 33 is coaxially connected to a stirring shaft, and the stirring blades 21 are evenly distributed on the stirring shaft. The rotary motor 33 drives the stirring blades 21 to rotate, performing mechanical stirring. In addition, the first bracket 321 is provided with a clamp for fixing the acid-base storage container 23. The acid-base storage container 23 is fixed by the clamp, and when it is necessary to replace a different acid-base storage container 23, it is only necessary to unfasten the clamp and reinstall and fix the new acid-base storage container 23, which is convenient to use. Both the pH probe 24 and the thermometer 25 are connected to the second bracket 322 via power cords, and the connection points are located outside the container 20. This way, when no testing is needed, the pH probe 24 and thermometer 25 are placed outside the container 20, unaffected by the container 20 or the high-temperature solution inside. When needed, the solution temperature has roughly cooled to room temperature, preventing negative impacts on the pH probe 24, thermometer 25, and power cord. They can then be manually moved into the container 20 for testing. The control panel 10 is mounted on the second bracket 322 and located outside the container 20 to avoid being affected by the solution inside the container 20.

[0026] In one embodiment, the cooling structure includes a condensate coil 22 wound around the surface of the container 20. The condensate coil 22 is connected to a cold source 221, and a water pump is installed on the condensate coil 22. Coolant in the cold source 221 flows into the condensate coil 22 under the action of the water pump, and then flows out from the other end of the condensate coil 22 back into the cold source 221, forming a complete cooling circulation system. The container 20 is cooled through the condensate coil 22. The water pump is electrically connected to the control panel 10, and the start and stop times of the water pump can be controlled through the control panel 10, that is, the timing of cooling the container 20 can be controlled.

[0027] In addition, the electromagnetic heating element on the side may have a negative impact on cooling during the heating process. Therefore, during the gelatinization process, no coolant is introduced into the condensate coil 22, and the valves at both ends of the condensate coil 22 are locked. After the gelatinization reaction is completed, the valves are opened, and coolant is introduced into the condensate coil 22 by the action of a water pump. This can reduce the impact of the initial heating on the subsequent cooling.

[0028] Alternatively, an insulation layer can be wrapped around the outer surface of the container 20 or the outer surface of the electromagnetic heating plate or electromagnetic heating layer. In this way, the condensate coil 22 can always be filled with coolant, so that the initial heating and the later cooling will not affect each other.

[0029] In one embodiment, the container 20 is a stainless steel cylindrical container with an arc-shaped bottom. The leakage valve 26 is installed at the bottom of the arc-shaped bottom to release the culture medium inside the container 20 as completely as possible, thus avoiding waste of resources caused by leaving it on the bottom wall of the container.

[0030] In one embodiment, the leakage valve 26 is electrically connected to the control panel 10, and the control panel 10 controls the leakage amount (or leakage volume) and leakage interval of the leakage valve 26. The bottom of the container 20 is provided with multiple legs 27, which support the container 20 to a predetermined height. The culture medium preparation device also includes a conveyor belt 40 that can stop at intervals. The surface of the conveyor belt 40 is evenly spaced with triangular bottle marking points 41 for placing triangular bottles at designated points. Simultaneously, the leakage interval of the leakage valve 26 is matched with the conveying speed of the conveyor belt 40 and the interval distance of the triangular bottle marking points 41, thereby achieving automatic dispensing and conveying of the culture medium, automatically dispensing the culture medium in the container 20 into multiple triangular bottles, resulting in a higher level of automation. The intermittently stopping conveyor belt 40 is existing technology, therefore its specific structure is not described in detail. It can be implemented using either an "incomplete gear mechanism" or a "motor + frequency converter / servo drive + position detection" system. For example, in an incomplete gear mechanism, the driving wheel has only a portion of its teeth, with the rest being toothless. When the toothed parts mesh, the driven wheel rotates, and the conveyor belt 40 rotates; when the toothless parts are engaged, the driven wheel stops, and the conveyor belt 40 stops. This intermittent stopping of the conveyor belt 40 is achieved through the cooperation of the driving wheel, driven wheel, and conveyor belt 40. Alternatively, in a "motor + frequency converter / servo drive + position detection" system, the conveyor belt 40 is driven by a motor (usually a three-phase asynchronous motor or a servo motor). The motor's start and stop are controlled by the frequency converter or servo drive. A photoelectric sensor or proximity switch is installed at the desired stopping position (for position detection). When an item arrives, the sensor sends a signal, and the controller commands the motor to stop, thus achieving intermittent stopping of the conveyor belt 40.

[0031] It should be noted that the terms "first" and "second" used in this application are for descriptive purposes only and do not indicate any order. They should not be construed as indicating or implying relative importance, and can be interpreted as names.

[0032] Those skilled in the art should understand that the embodiments of the present invention described above and shown in the accompanying drawings are merely examples and do not limit the present invention. The advantages of the present invention have been fully and effectively realized. The functions and structural principles of the present invention have been shown and explained in the embodiments, and any modifications or variations may be made to the implementation of the present invention without departing from the stated principles.

Claims

1. A culture medium preparation device, characterized in that, The device includes a control panel and a container. The surface of the container is marked with solution volume measurement scales. A stirring blade is located near the bottom of the container. Electromagnetic heating elements are located at the bottom and sides of the container. A cooling structure is located on the outer wall of the container. A support is connected to the side wall of the container. The support protrudes upward from the container and is equipped with an acid / base reservoir, a pH probe, and a thermometer. A dropper is located at the bottom of the acid / base reservoir for dripping acid or alkali solution into the container. The pH probe and the thermometer are used to detect the pH value and temperature of the solution in the container, respectively. A leakage valve is located at the bottom of the container. The control panel is used to control the rotation of the stirring blades, to control the heating of the electromagnetic heating element, and to control the cooling structure to cool the container.

2. The media formulation device of claim 1, wherein, The electromagnetic heating element is integrally disposed within the bottom wall and side wall of the container.

3. The media formulation device of claim 1, wherein, The bottom and sides of the container are provided with electromagnetic heating plates or electromagnetic heating layers, and the electromagnetic heating elements are located inside the electromagnetic heating plates or electromagnetic heating layers.

4. The media formulation device of claim 1, wherein, The bracket includes a connecting part and a supporting part. The supporting part is located above the container and is connected to the container through the connecting part. The supporting part includes a first bracket and a second bracket connected in a cross shape. A rotary motor is installed at the center of the first bracket. The rotating shaft at the bottom of the rotary motor is coaxially connected to a stirring shaft. The stirring blades are evenly distributed on the stirring shaft. The first bracket is provided with a clamp for fixing the acid-base storage container. The pH probe and the thermometer are both connected to the second bracket through a power cord. The connection position is located outside the container. The control panel is located on the second bracket and outside the container.

5. The media formulation device of claim 1, wherein, The cooling structure includes a condensate coil wound around the surface of the container, the condensate coil being connected to a cold source, and a water pump being installed on the condensate coil, the water pump being electrically connected to the control panel.

6. The media formulation device of claim 1, wherein, The container is a stainless steel cylindrical container with an arc-shaped bottom, and the leakage valve is installed at the bottom end of the arc-shaped bottom.

7. The media formulation device of claim 6, wherein, The leakage valve is electrically connected to the control panel. The bottom of the container is provided with multiple legs to support the container to a predetermined height. The culture medium preparation device also includes a conveyor belt that can stop at intervals. The surface of the conveyor belt is evenly spaced with triangular bottle marking points for placing triangular bottles at designated points.

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