Process for extracting and refining flavone in highland barley seedlings by enzymolysis-membrane ultrafiltration

Abstract

The invention relates to the technical field of extraction and refinement of flavonoids compounds in highland barley seedlings, in particular to a process for extracting and refining flavone in highland barley seedlings by enzymolysis-membrane ultrafiltration. The process comprises the following six steps: pretreatment, beating, enzymolysis, extraction, ultrafiltration and drying; wherein in the step of pretreatment, fresh highland barley seedlings with the growth height of 25-40cm are cut; the weight ratio of cellulose to the highland barley seedlings is 1: 100-1000, the temperature is 30-60DEG C, the PH value is 3.5-6, and the stirring time is 0.5-4 hours; in the step of extraction, the weight ratio of highland barley seedling slurry to ethanol of 40-95 percent is 1: 3-10, reflux extraction is carried out for 1-3 hours, and extract obtained in twic is emerged; and in the step of ultrafiltration, the extract is respectively micro-filtered by microfiltration membranes of 1, 0.5 and 0.1mu m, micro-filter filtrate is ultra-filtered by the ultrafiltration membranes, the operating pressure is 0.15-0.25MPa, the temperature is 25-45DEG C, the ultra-filter filtrate is ultra-filtered by the ultrafiltration membranes, the operating pressure is 0.15-0.25MPa, the temperature is 25-45DEG C, and the filtrate is collected. The process is convenient, and the flavone has high yield, high purity and remained activity.

Description

[0001] 1. Technical field: the present invention belongs to the technical field of extraction and refining methods of flavonoids in highland barley seedlings, and in particular relates to a process for extracting and refining total flavonoids of highland barley seedlings by enzymolysis-membrane ultrafiltration. 2. Background technology

[0002] Flavonoids, also known as flavonoids, mainly refer to the general term of a class of compounds with chromone ring and benzene ring as the basic structure, with a C6-C3-C6 basic configuration, and are metabolites of plant polyphenols. A class of compounds that people pay more attention to. Flavonoids have a wide range of biological activities, including anti-oxidation, anti-mutation, anti-aging, anti-tumor, antibacterial, etc. Extracting and refining flavonoids has become a hot spot in the development and utilization of natural products at home and abroad.

[0003] Highland barley (Hordeum vulgare L.var.nudum Hook.F.) is a cereal crop be...

Examples

Embodiment 1

[0017] Embodiment 1: In the pretreatment, the fresh highland barley seedlings with a growth height of about 25 cm are cut and selected to remove withered leaf seedlings, soil and weeds. Select highland barley seedlings that have no air pollution, no soil pollution, and no chemical fertilizers; wash the selected highland barley seedlings with water before use. During beating, the highland barley seedlings are processed with a beater to make highland barley seedling slurry. During enzymolysis, add cellulase to the highland barley seedling slurry. The enzymatic hydrolysis parameters are: the weight ratio of cellulase to highland barley seedling slurry is 1:100, the temperature is about 30°C, the pH value is 3.5, and the stirring time for enzymolysis is 0.5 hours. . The adjustment of pH value was adjusted with hydrochloric acid. During the extraction, add a concentration of 40% ethanol solution to the highland barley seedling slurry that has been enzymatically hydrolyzed, the we...

Embodiment 2

[0018] Embodiment 2: In the pretreatment, the fresh highland barley seedlings with a growth height of about 40 cm are cut and selected to remove withered leaf seedlings, soil and weeds. Wash the selected highland barley seedlings with water before use. During beating, the highland barley seedlings are processed with a beater to make highland barley seedling slurry. In the enzymolysis, add cellulase to the highland barley seedling slurry. The enzymatic hydrolysis parameters are: the weight ratio of cellulase to highland barley seedling slurry is 1:1000, the temperature is about 60°C, the pH value is 6, and the enzymatic hydrolysis time is 4 hours. , The adjustment of pH value is adjusted with acetic acid. During the extraction, add a 95% ethanol solution to the enzymatically hydrolyzed highland barley seedling slurry, the weight ratio of the highland barley seedling slurry to the ethanol solution is 1:10, and after reflux extraction for about 3 hours, centrifuge; repeat the ab...

Embodiment 3

[0019] Embodiment 3: In the pretreatment, the fresh highland barley seedlings with a growth height of about 30 cm are cut and selected to remove withered leaf seedlings, soil and weeds. Wash the selected highland barley seedlings with water before use. During beating, the highland barley seedlings are processed with a beater to make highland barley seedling slurry. In the enzymolysis, add cellulase to the highland barley seedling slurry, the enzymatic hydrolysis parameters are: the weight ratio of cellulase to the highland barley seedling slurry is 1:500, the temperature is about 50°C, the pH value is 5, and the enzymatic hydrolysis time is 3 hours , The adjustment of pH value is adjusted with acetic acid. During the extraction, add the ethanol solution with a concentration of 75% to the highland barley seedling slurry that has been enzymatically hydrolyzed. The weight ratio of the highland barley seedling slurry to the ethanol solution is 1:8, and after reflux extraction for...

[0001] 1. Technical field: the present invention belongs to the technical field of extraction and refining methods of flavonoids in highland barley seedlings, and in particular relates to a process for extracting and refining total flavonoids of highland barley seedlings by enzymolysis-membrane ultrafiltration. 2. Background technology

[0002] Flavonoids, also known as flavonoids, mainly refer to the general term of a class of compounds with chromone ring and benzene ring as the basic structure, with a C6-C3-C6 basic configuration, and are metabolites of plant polyphenols. A class of compounds that people pay more attention to. Flavonoids have a wide range of biological activities, including anti-oxidation, anti-mutation, anti-aging, anti-tumor, antibacterial, etc. Extracting and refining flavonoids has become a hot spot in the development and utilization of natural products at home and abroad.

[0003] Highland barley (Hordeum vulgare L.var.nudum Hook.F.) is a cereal crop be...

Examples

Embodiment 1

[0017] Embodiment 1: In the pretreatment, the fresh highland barley seedlings with a growth height of about 25 cm are cut and selected to remove withered leaf seedlings, soil and weeds. Select highland barley seedlings that have no air pollution, no soil pollution, and no chemical fertilizers; wash the selected highland barley seedlings with water before use. During beating, the highland barley seedlings are processed with a beater to make highland barley seedling slurry. During enzymolysis, add cellulase to the highland barley seedling slurry. The enzymatic hydrolysis parameters are: the weight ratio of cellulase to highland barley seedling slurry is 1:100, the temperature is about 30°C, the pH value is 3.5, and the stirring time for enzymolysis is 0.5 hours. . The adjustment of pH value was adjusted with hydrochloric acid. During the extraction, add a concentration of 40% ethanol solution to the highland barley seedling slurry that has been enzymatically hydrolyzed, the we...

Embodiment 2

[0018] Embodiment 2: In the pretreatment, the fresh highland barley seedlings with a growth height of about 40 cm are cut and selected to remove withered leaf seedlings, soil and weeds. Wash the selected highland barley seedlings with water before use. During beating, the highland barley seedlings are processed with a beater to make highland barley seedling slurry. In the enzymolysis, add cellulase to the highland barley seedling slurry. The enzymatic hydrolysis parameters are: the weight ratio of cellulase to highland barley seedling slurry is 1:1000, the temperature is about 60°C, the pH value is 6, and the enzymatic hydrolysis time is 4 hours. , The adjustment of pH value is adjusted with acetic acid. During the extraction, add a 95% ethanol solution to the enzymatically hydrolyzed highland barley seedling slurry, the weight ratio of the highland barley seedling slurry to the ethanol solution is 1:10, and after reflux extraction for about 3 hours, centrifuge; repeat the ab...

Embodiment 3

[0019] Embodiment 3: In the pretreatment, the fresh highland barley seedlings with a growth height of about 30 cm are cut and selected to remove withered leaf seedlings, soil and weeds. Wash the selected highland barley seedlings with water before use. During beating, the highland barley seedlings are processed with a beater to make highland barley seedling slurry. In the enzymolysis, add cellulase to the highland barley seedling slurry, the enzymatic hydrolysis parameters are: the weight ratio of cellulase to the highland barley seedling slurry is 1:500, the temperature is about 50°C, the pH value is 5, and the enzymatic hydrolysis time is 3 hours , The adjustment of pH value is adjusted with acetic acid. During the extraction, add the ethanol solution with a concentration of 75% to the highland barley seedling slurry that has been enzymatically hydrolyzed. The weight ratio of the highland barley seedling slurry to the ethanol solution is 1:8, and after reflux extraction for...