Diatom carrier culture technique

Abstract

The invention relates to a high-density and large-scale diatom carrier culture technique which comprises the steps that many kinds of seaweed meal serve as carriers; 0.001-50% of seaweed meal is added to seawater; an appropriate amount of nutritive salt is added; a diatom seed solution is inoculated; natural or artificial light is adopted; the diatom is cultured at a natural temperature of 30 DEGC for 2-30 days; and ventilation or agitation is performed appropriately in a culture process. Diatom is attached to the surface of seaweed, grows and propagates. After the culture, a seaweed and diatom mixture is separated and obtained by separation methods such as natural settling, filtering and centrifugation, contains more than thirty million diatom cells per gram in wet weight, and can serve as fresh and alive fish bait to be fed directly. The separated seawater can be used for the culture next time directly after the nutritive salt, the seaweed meal and the diatom seed solution are added. The culture technique is convenient and easy to implement, and is applicable to reactor culture and large-scale pond culture of benthic diatom; high-density fresh and alive diatom biomass can be obtained; and the alive diatom cells serve as feed to be fed, and can exert a function of improving a substrate environment.

Description

(1) Technical field

[0001] The invention relates to the field of microalgae cultivation, in particular to a technology for high-density and large-scale cultivation of diatom carriers. (2) Background technology

[0002] Diatoms are widely distributed and various in variety, rich in nutrients and easy to digest. They are the main bait for fish, shellfish and shrimp, especially their larvae. Together with other plants, they constitute the primary productivity of water ecosystems. Diatoms can synthesize a large amount of polysaccharides and highly unsaturated fatty acids (PUFA), among which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are essential fatty acids for human body. important role in many diseases. In aquaculture, EPA and DHA are necessary for the growth and development of aquatic economic animal larvae. The abnormal diseases, low survival rate and poor vitality of many aquatic economic animal larvae are related to the lack of EPA and DHA in the bait. A...

Examples

Embodiment 1

[0018] Erlenmeyer flask culture of benthic diatoms

[0019] Cladophora was crushed and passed through a 20-mesh sieve. Seawater is filtered through a 0.22 μm microporous membrane, f / 2 medium is used, 0.5-2.0% sargassum powder is added to the water body, and activated benthic diatoms are inoculated, and the initial density of diatoms is 10 4 cell / ml, cultured at 23°C under continuous light and without ventilation, stirred 3 times a day, and cultured for 5-10 days. After the cultivation, the algae are separated by filtration, and each gram of wet weight contains 65 million diatom cells.

Embodiment 2

[0021] Reactor Cultivation of Benthic Diatoms

[0022] Sage algae was dried and crushed to 40 mesh. Seawater is filtered through a 0.45 μm microporous membrane, diatom-optimized medium is used, 0.1-5.0% Clado algae powder is added to the water body, and flat photobioreactors with optical paths of 30 mm, 50 mm and 100 mm are used for suspension culture. Inoculate activated benthic diatoms, the initial density of diatoms is 10 4 cell / ml, cultured at 23°C for 5-7 days under natural light and ventilation. After the cultivation, the algae are separated by static sedimentation, and each gram of wet weight contains more than 85 million diatom cells.

[0023] Add 0.1-5.0% Cladophora powder and an appropriate amount of diatom-optimized medium to the separated upper water body, carry out secondary culture in the original reactor under the same conditions, and obtain algae powder containing more than 80 million diatom cells per gram of wet weight.

[0024] The above-mentioned fresh an...

Embodiment 3

[0026] Pond cultivation of benthic diatoms

[0027] Sargassum dried and crushed through a 20-mesh sieve. The seawater is introduced into the culture pond (water depth 1.5m) after sand filtration, add 0.5-10% sargassum and appropriate amount of urea, inoculate the activated benthic diatoms, the initial density of diatoms is 10 3 cell / ml, cultured at natural temperature and natural light, ventilated twice a day in the morning and evening, 30 minutes each time, after 10-20 days of cultivation, the algae were drawn from the bottom of the pond, and after dewatering, each gram of wet weight contained 35 million diatom cells.

[0028] Put the above fresh bait directly into the water body to feed sea cucumbers. Under the same feeding mode, the survival rate of young ginseng is more than 50% higher than that of general feed, and the growth is good; adult ginseng has strong disease resistance, fast growth, and weight gain. The rate is high, which is 1.5-3.5 times faster than that of co...

(1) Technical field

[0001] The invention relates to the field of microalgae cultivation, in particular to a technology for high-density and large-scale cultivation of diatom carriers. (2) Background technology

[0002] Diatoms are widely distributed and various in variety, rich in nutrients and easy to digest. They are the main bait for fish, shellfish and shrimp, especially their larvae. Together with other plants, they constitute the primary productivity of water ecosystems. Diatoms can synthesize a large amount of polysaccharides and highly unsaturated fatty acids (PUFA), among which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are essential fatty acids for human body. important role in many diseases. In aquaculture, EPA and DHA are necessary for the growth and development of aquatic economic animal larvae. The abnormal diseases, low survival rate and poor vitality of many aquatic economic animal larvae are related to the lack of EPA and DHA in the bait. A...

Examples

Embodiment 1

[0018] Erlenmeyer flask culture of benthic diatoms

[0019] Cladophora was crushed and passed through a 20-mesh sieve. Seawater is filtered through a 0.22 μm microporous membrane, f / 2 medium is used, 0.5-2.0% sargassum powder is added to the water body, and activated benthic diatoms are inoculated, and the initial density of diatoms is 10 4 cell / ml, cultured at 23°C under continuous light and without ventilation, stirred 3 times a day, and cultured for 5-10 days. After the cultivation, the algae are separated by filtration, and each gram of wet weight contains 65 million diatom cells.

Embodiment 2

[0021] Reactor Cultivation of Benthic Diatoms

[0022] Sage algae was dried and crushed to 40 mesh. Seawater is filtered through a 0.45 μm microporous membrane, diatom-optimized medium is used, 0.1-5.0% Clado algae powder is added to the water body, and flat photobioreactors with optical paths of 30 mm, 50 mm and 100 mm are used for suspension culture. Inoculate activated benthic diatoms, the initial density of diatoms is 10 4 cell / ml, cultured at 23°C for 5-7 days under natural light and ventilation. After the cultivation, the algae are separated by static sedimentation, and each gram of wet weight contains more than 85 million diatom cells.

[0023] Add 0.1-5.0% Cladophora powder and an appropriate amount of diatom-optimized medium to the separated upper water body, carry out secondary culture in the original reactor under the same conditions, and obtain algae powder containing more than 80 million diatom cells per gram of wet weight.

[0024] The above-mentioned fresh an...

Embodiment 3

[0026] Pond cultivation of benthic diatoms

[0027] Sargassum dried and crushed through a 20-mesh sieve. The seawater is introduced into the culture pond (water depth 1.5m) after sand filtration, add 0.5-10% sargassum and appropriate amount of urea, inoculate the activated benthic diatoms, the initial density of diatoms is 10 3 cell / ml, cultured at natural temperature and natural light, ventilated twice a day in the morning and evening, 30 minutes each time, after 10-20 days of cultivation, the algae were drawn from the bottom of the pond, and after dewatering, each gram of wet weight contained 35 million diatom cells.

[0028] Put the above fresh bait directly into the water body to feed sea cucumbers. Under the same feeding mode, the survival rate of young ginseng is more than 50% higher than that of general feed, and the growth is good; adult ginseng has strong disease resistance, fast growth, and weight gain. The rate is high, which is 1.5-3.5 times faster than that of co...