A collagen fiber non-woven hydroxyapatite bioscaffold material

Abstract

The invention relates to a predation technology of an artificial bone repair material in the field of inorganic nonmetallic material and biomedical material, in particular to a collagenous fiber non-woven hydroxyapatite biological scaffold material and a preparation method thereof. The preparation method disclosed by the invention comprises the following steps: taking a collagenous fiber as a matrix, combining a non-woven needle punching technology with a biological biomimetic mineralization method, and uniformly depositing hydroxyapatite with the rod-shaped or wheat ear-shaped structure on the surface of the porous scaffold of the collagenous fiber. The collagenous fiber non-woven hydroxyapatite biological scaffold material prepared by the method has a three-dimensional connected macroporous structure with the pore diameter of 20-500 microns and the porosity of 10-95%, is high in interface bonding force between the collagenous fiber and the hydroxyapatite, achieves the tenacity of the collagenous fiber and the strength of hydroxyapatite simultaneously, and is excellent in mechanical properties. The constituents and the microstructure of the scaffold material are both similar to a natural bone, so as to facilitate differentiation and osteogenesis of mesenchymal stem cells of a human body.

Description

technical field

[0001] The invention relates to the preparation technology of artificial bone repair materials in the field of inorganic non-metallic materials and biomedical materials, in particular to a collagen fiber non-woven hydroxyapatite biological scaffold material and a preparation method thereof. Background technique

[0002] Large segmental bone defects caused by traffic accidents, trauma, surgical resection, etc. are currently one of the most common and difficult problems in clinical medicine at home and abroad. In recent years, the development of bone tissue engineering research has provided a new idea and method for repairing large bone defects. In bone tissue engineering, bio-scaffold material occupies a very important position. It not only supports and maintains the shape of the original tissue, but also acts as a template, providing a place for cells to lodge, grow, differentiate and proliferate. , thereby guiding the regeneration of damaged tissue and cont...

Examples

Embodiment 1

[0035] (1) Three-dimensional porous scaffolds of collagen fibers were prepared by acupuncture molding process:

[0036] 100g / m 2 After the collagen fibers are mixed and loosened, they are combed into a fiber web, and three of the fiber webs are laid in parallel so that the fibers are arranged in a longitudinal direction, and are reinforced by acupuncture. The acupuncture frequency is 1200 pricks / minute, and the acupuncture depth is 3mm. , cut the collagen fiber three-dimensional porous scaffold into the same shape as the required bone repair material, a square with a side length of 2 cm and a thickness of 3 mm, and the pore diameter of the scaffold is 20-200 μm. and dried in a drying oven at 40°C to obtain a three-dimensional porous scaffold of collagen fibers.

[0037] (2) The surface of the collagen fiber three-dimensional porous scaffold is enriched with calcium ions and phosphate ions:

[0038]Weigh 2.0g collagen and dissolve it in 100mL 4vt% acetic acid solution, stir c...

Embodiment 2

[0049] (1) Three-dimensional porous scaffolds of collagen fibers were prepared by acupuncture molding process:

[0050] 120g / m 2 After the collagen fibers are mixed and loosened, they are combed into a fiber web, and two of the fiber webs are laid in parallel so that the fibers are arranged in a longitudinal direction, and are reinforced by acupuncture. The acupuncture frequency is 1000 pricks / minute, and the acupuncture depth is 3mm. , cut the collagen fiber three-dimensional porous scaffold into the same shape as the required bone repair material, a square with a side length of 2 cm and a thickness of 3 mm, and the pore diameter of the scaffold is 40-200 μm. and dried in a drying oven at 30°C to obtain a three-dimensional porous scaffold of collagen fibers.

[0051] (2) The surface of the collagen fiber three-dimensional porous scaffold is enriched with calcium ions and phosphate ions:

[0052] Weigh 4.0g collagen and dissolve it in 100mL 4vt% acetic acid solution, stir co...

Embodiment 3

[0057] (1) The three-dimensional porous scaffold of collagen fibers was prepared by the acupuncture molding process as in Example 1.

[0058] (2) Weigh 4.0g collagen and dissolve it in 100mL 4vt% acetic acid solution, stir continuously to dissolve it completely, then weigh 1.1098g CaCl according to the calcium-phosphorus ratio of 1.67:1 2 with 0.6888g NH 4 h 2 PO 4 Dissolve in it; immerse the collagen fiber three-dimensional porous scaffold prepared in (1) into the above solution, and ultrasonically disperse it for 5 minutes, slowly pull out the scaffold at a speed of 5mm / s with tweezers, and place it in a 40°C drying oven Medium dry.

[0059] (3) Depositing hydroxyapatite on the surface of collagen fiber three-dimensional porous scaffold by biomineralization method:

[0060] Place the three-dimensional porous scaffold of collagen fibers enriched in calcium ions and phosphate ions obtained in step (2) in a medium containing 3.12g NaH 2 PO 4 2H 2 In the NaOH alkaline sol...

technical field

[0001] The invention relates to the preparation technology of artificial bone repair materials in the field of inorganic non-metallic materials and biomedical materials, in particular to a collagen fiber non-woven hydroxyapatite biological scaffold material and a preparation method thereof. Background technique

[0002] Large segmental bone defects caused by traffic accidents, trauma, surgical resection, etc. are currently one of the most common and difficult problems in clinical medicine at home and abroad. In recent years, the development of bone tissue engineering research has provided a new idea and method for repairing large bone defects. In bone tissue engineering, bio-scaffold material occupies a very important position. It not only supports and maintains the shape of the original tissue, but also acts as a template, providing a place for cells to lodge, grow, differentiate and proliferate. , thereby guiding the regeneration of damaged tissue and cont...

Examples

Embodiment 1

[0035] (1) Three-dimensional porous scaffolds of collagen fibers were prepared by acupuncture molding process:

[0036] 100g / m 2 After the collagen fibers are mixed and loosened, they are combed into a fiber web, and three of the fiber webs are laid in parallel so that the fibers are arranged in a longitudinal direction, and are reinforced by acupuncture. The acupuncture frequency is 1200 pricks / minute, and the acupuncture depth is 3mm. , cut the collagen fiber three-dimensional porous scaffold into the same shape as the required bone repair material, a square with a side length of 2 cm and a thickness of 3 mm, and the pore diameter of the scaffold is 20-200 μm. and dried in a drying oven at 40°C to obtain a three-dimensional porous scaffold of collagen fibers.

[0037] (2) The surface of the collagen fiber three-dimensional porous scaffold is enriched with calcium ions and phosphate ions:

[0038]Weigh 2.0g collagen and dissolve it in 100mL 4vt% acetic acid solution, stir c...

Embodiment 2

[0049] (1) Three-dimensional porous scaffolds of collagen fibers were prepared by acupuncture molding process:

[0050] 120g / m 2 After the collagen fibers are mixed and loosened, they are combed into a fiber web, and two of the fiber webs are laid in parallel so that the fibers are arranged in a longitudinal direction, and are reinforced by acupuncture. The acupuncture frequency is 1000 pricks / minute, and the acupuncture depth is 3mm. , cut the collagen fiber three-dimensional porous scaffold into the same shape as the required bone repair material, a square with a side length of 2 cm and a thickness of 3 mm, and the pore diameter of the scaffold is 40-200 μm. and dried in a drying oven at 30°C to obtain a three-dimensional porous scaffold of collagen fibers.

[0051] (2) The surface of the collagen fiber three-dimensional porous scaffold is enriched with calcium ions and phosphate ions:

[0052] Weigh 4.0g collagen and dissolve it in 100mL 4vt% acetic acid solution, stir co...

Embodiment 3

[0057] (1) The three-dimensional porous scaffold of collagen fibers was prepared by the acupuncture molding process as in Example 1.

[0058] (2) Weigh 4.0g collagen and dissolve it in 100mL 4vt% acetic acid solution, stir continuously to dissolve it completely, then weigh 1.1098g CaCl according to the calcium-phosphorus ratio of 1.67:1 2 with 0.6888g NH 4 h 2 PO 4 Dissolve in it; immerse the collagen fiber three-dimensional porous scaffold prepared in (1) into the above solution, and ultrasonically disperse it for 5 minutes, slowly pull out the scaffold at a speed of 5mm / s with tweezers, and place it in a 40°C drying oven Medium dry.

[0059] (3) Depositing hydroxyapatite on the surface of collagen fiber three-dimensional porous scaffold by biomineralization method:

[0060] Place the three-dimensional porous scaffold of collagen fibers enriched in calcium ions and phosphate ions obtained in step (2) in a medium containing 3.12g NaH 2 PO 4 2H 2 In the NaOH alkaline sol...

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