A method for inducing loose callus of Dracaena glabra

Abstract

A method for inducing loose callus of Dracaena phoenix leaf, comprising the steps of: (1) taking the seeds or buds of Dracaena phoenix leaf respectively as explants for disinfection; (2) placing the sterilized explants in Induce buds or bud clusters in MS basic medium; (3) place sterile buds in MS, B5, N6 respectively, add 6-benzyl adenine 6-BA and naphthalene acetic acid NAA in the culture medium to obtain callus (4) The callus was placed in the induction medium supplemented with different hormones and cultured to obtain more callus with loose texture. The callus obtained by the method of the present invention is tender green or milky white, loose, and has strong proliferation ability, and can induce a large amount of well-grown loose callus in a short time, for the production of total alkaloids of Dracaena glabrata for cell suspension culture provide materials.

Description

technical field

[0001] The invention relates to a method for inducing plant loose callus, in particular to a method for inducing loose callus of Dracaena glabrata. Background technique

[0002] Dracaena cochinchinensis (Lour) S.C. Chen, also known as Millennium Wood, Blood Dracaena, and Cochin Dracaena, is a endangered species and is a national second-class protected plant. Dracaena fragrans has high medicinal value, and the blood jelly extracted from its resin is called kylin jelly. It is a traditional Chinese medicinal material and has been used in my country for more than 1,500 years. According to the "Compendium of Materia Medica", dragon's blood is warm, flat, sweet, salty, non-toxic, enters the blood system, and returns to the lung, spleen, and kidney meridians. It has remarkable effects such as promoting muscle growth and suppressing sores, and has the reputation of "the holy medicine for promoting blood circulation". It is mainly distributed in Yunnan, Guangxi, Hain...

Examples

Embodiment 1

[0017] An example of the method for the induction of the loose callus of Dracaena glabrata of the present invention, comprises the steps:

[0018] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;

[0019] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a...

Embodiment 2

[0023] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;

[0024] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a culture temperature of 25±2°C, with a light intensity of 20-30 μmol / m 2 s, cultivated under the condition of light time of 12h / d, and sprou...

Embodiment 3

[0028] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;

[0029] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a culture temperature of 25±2°C, with a light intensity of 20-30 μmol / m 2 s, cultivated under the condition of light time of 12h / d, and sprou...

technical field

[0001] The invention relates to a method for inducing plant loose callus, in particular to a method for inducing loose callus of Dracaena glabrata. Background technique

[0002] Dracaena cochinchinensis (Lour) S.C. Chen, also known as Millennium Wood, Blood Dracaena, and Cochin Dracaena, is a endangered species and is a national second-class protected plant. Dracaena fragrans has high medicinal value, and the blood jelly extracted from its resin is called kylin jelly. It is a traditional Chinese medicinal material and has been used in my country for more than 1,500 years. According to the "Compendium of Materia Medica", dragon's blood is warm, flat, sweet, salty, non-toxic, enters the blood system, and returns to the lung, spleen, and kidney meridians. It has remarkable effects such as promoting muscle growth and suppressing sores, and has the reputation of "the holy medicine for promoting blood circulation". It is mainly distributed in Yunnan, Guangxi, Hain...

Examples

Embodiment 1

[0017] An example of the method for the induction of the loose callus of Dracaena glabrata of the present invention, comprises the steps:

[0018] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;

[0019] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a...

Embodiment 2

[0023] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;

[0024] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a culture temperature of 25±2°C, with a light intensity of 20-30 μmol / m 2 s, cultivated under the condition of light time of 12h / d, and sprou...

Embodiment 3

[0028] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;

[0029] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a culture temperature of 25±2°C, with a light intensity of 20-30 μmol / m 2 s, cultivated under the condition of light time of 12h / d, and sprou...