Preservation method of chlorella concentrate

Abstract

The invention discloses a preservation method of chlorella concentrate, comprising the following steps: (1), flocculating chlorella liquid respectively with 120-200 mg / L of alums and 30-60 mg / L of iron chloride, centrifuging and concentrating chlorella to prepare chlorella concentrate, and storing at 4 DEG C for use; (2), subpackaging the prepared chlorella concentrate in 5ml freeze-storing tubes, sealing the free-storing tubes where the chlorella concentrate is loaded, with black plastic bags, placing in a refrigerator at 4 DEG C, and storing for 3 months. The preservation method is simple to perform, good in preserving effect, widely applicable and low in cost.

Description

technical field

[0001] The invention relates to the technical field of marine organisms, in particular to a method for preserving a chlorella concentrate. Background technique

[0002] Microalgae are single-celled eukaryotic organisms with high photosynthetic utilization rate. The size is generally between 5 and 25 μm. It is impossible to observe its shape, structure, size and activity mode directly with the naked eye. It needs to be distinguished through a microscope. Cell shape, structure, etc., hence the name microalgae. It is widely distributed in various temperature zones, and there are microalgae from fresh water to sea water. Because of its strong photosynthesis, fast proliferation, easy artificial cultivation, and rich nutrition, microalgae are now widely used in aquaculture, drug research and development, sewage purification, food industry, pigment extraction, and energy development. Most microalgae are rich in polyunsaturated fatty acids such as eicosapentaenoic ...

Examples

Embodiment 1

[0017] A kind of preservation method of chlorella concentrate of the present invention comprises the following steps:

[0018] (1) Use 120mg / L of alum and 30mg / L of ferric chloride to flocculate the chlorella algae liquid respectively, centrifuge and concentrate the chlorella, prepare a chlorella concentrate and store it at 4°C for later use;

[0019] (2) Divide the prepared chlorella concentrate into 5ml cryopreservation tubes, then seal the cryopreservation tubes containing the chlorella concentrate with black plastic bags and place them in refrigerators at 4°C for storage. The time is 3 months. Example 2

Embodiment 2

[0020] The difference between embodiment 2 and embodiment 1 is: the preservation method of a kind of chlorella concentrate of the present invention, comprises the following steps: in step (1), use the alum of 180mg / L, the chlorination of 40mg / L The chlorella liquid is flocculated with iron, the chlorella is concentrated by centrifugation, and the chlorella concentrate is prepared and stored at 4°C for later use.

Embodiment 3

[0022] The difference between embodiment 3 and embodiment 1 is: a kind of preservation method of chlorella concentrate of the present invention comprises the following steps: in step (1), respectively use the alum of 200mg / L, the chlorination of 60mg / L Iron flocculation of chlorella algae liquid, centrifugal concentration of chlorella, prepared into chlorella concentrate and stored at 4°C for later use;

technical field

[0001] The invention relates to the technical field of marine organisms, in particular to a method for preserving a chlorella concentrate. Background technique

[0002] Microalgae are single-celled eukaryotic organisms with high photosynthetic utilization rate. The size is generally between 5 and 25 μm. It is impossible to observe its shape, structure, size and activity mode directly with the naked eye. It needs to be distinguished through a microscope. Cell shape, structure, etc., hence the name microalgae. It is widely distributed in various temperature zones, and there are microalgae from fresh water to sea water. Because of its strong photosynthesis, fast proliferation, easy artificial cultivation, and rich nutrition, microalgae are now widely used in aquaculture, drug research and development, sewage purification, food industry, pigment extraction, and energy development. Most microalgae are rich in polyunsaturated fatty acids such as eicosapentaenoic ...

Examples

Embodiment 1

[0017] A kind of preservation method of chlorella concentrate of the present invention comprises the following steps:

[0018] (1) Use 120mg / L of alum and 30mg / L of ferric chloride to flocculate the chlorella algae liquid respectively, centrifuge and concentrate the chlorella, prepare a chlorella concentrate and store it at 4°C for later use;

[0019] (2) Divide the prepared chlorella concentrate into 5ml cryopreservation tubes, then seal the cryopreservation tubes containing the chlorella concentrate with black plastic bags and place them in refrigerators at 4°C for storage. The time is 3 months. Example 2

Embodiment 2

[0020] The difference between embodiment 2 and embodiment 1 is: the preservation method of a kind of chlorella concentrate of the present invention, comprises the following steps: in step (1), use the alum of 180mg / L, the chlorination of 40mg / L The chlorella liquid is flocculated with iron, the chlorella is concentrated by centrifugation, and the chlorella concentrate is prepared and stored at 4°C for later use.

Embodiment 3

[0022] The difference between embodiment 3 and embodiment 1 is: a kind of preservation method of chlorella concentrate of the present invention comprises the following steps: in step (1), respectively use the alum of 200mg / L, the chlorination of 60mg / L Iron flocculation of chlorella algae liquid, centrifugal concentration of chlorella, prepared into chlorella concentrate and stored at 4°C for later use;

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