A kind of method for detoxification and rejuvenation of Hemerocallis germplasm

Abstract

The invention provides a day lily germplasm detoxification and rejuvenation method. The day lily germplasm detoxification and rejuvenation method comprises the following detail steps: (1) selecting to-be-inoculated flower buds of day lily; (2) preparing a day lily anther induction medium; (3) disinfecting and inoculating anthers; (4) carrying out anther induction culture; (5) carrying out anther-derived callus differentiation and rooting culture; (6) carrying out hardening-seedling and transplanting of day lily seedlings; (7) removing non-diploid day lily plants. According to the day lily germplasm detoxification and rejuvenation method provided by the invention, day lily pollen plants are obtained through a day lily anther culture way, so as to further obtain day lily diploid plants by removing non-diploid day lily abnormal plants from the anther cultural population, the germplasm detoxification and rejuvenation method is significant in detoxification effect, the day lily germplasm can be rejuvenated, and the quality and yield of day lily are greatly improved.

Description

technical field

[0001] The invention relates to the field of garden science, in particular to a method for detoxification and rejuvenation of daylily germplasm by efficiently removing daymerocallis virus through an anther culture approach, thereby greatly improving the quality and yield of daylily germplasm. Background technique

[0002] Hemerocallis fulva (Hemerocallis fulva) is a perennial herb of Liliaceae and Hemerocallis genus. It is also known as day lily, forget worry grass, Yiman grass, etc. It is native to China and is called the "mother flower" of China. Hemerocallis has many varieties, bright colors and beautiful flower shapes, and is widely loved by the people. "Compendium of Materia Medica" said: "Hemerocallis is originally made of stalks. Scallops, forgetting." The ancients thought that daylilies could make people forget their worries, so they called them "Forget Worry Grass". "Fengtuji" records: "'Pregnant women who wear the grass will give birth to boys', so...

Examples

Embodiment 1

[0054] In mid-June 2014, the anthers of the diploid Hemerocallis species Double Firecracker and Golden Baby were selected for flower cultivation to induce detoxification, and the detailed steps are as follows:

[0055] (1) Selection of flower buds to be inoculated

[0056] On June 25th, when the double-petalled firecrackers and golden dolls were budding and blooming, most of the anthers were in the late mononucleate flower buds, and they were brought back to the laboratory with a curling bottle, wrapped in moist gauze, and placed in a refrigerator at 4°C for low temperature pretreatment. Process 4d;

[0057] (2) Preparation of Hemerocallis anther induction medium

[0058] Hemerocallis anther induction medium is prepared from the following substances per liter of distilled water:

[0059] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ∙7H 2 O 370mg / L, CaCl 2 ∙2H 2 O 840mg / L;

[0060] Trace elements: MnSO 4 ∙4H 2 O 22.5mg / L, ZnSO 4...

technical field

[0001] The invention relates to the field of garden science, in particular to a method for detoxification and rejuvenation of daylily germplasm by efficiently removing daymerocallis virus through an anther culture approach, thereby greatly improving the quality and yield of daylily germplasm. Background technique

[0002] Hemerocallis fulva (Hemerocallis fulva) is a perennial herb of Liliaceae and Hemerocallis genus. It is also known as day lily, forget worry grass, Yiman grass, etc. It is native to China and is called the "mother flower" of China. Hemerocallis has many varieties, bright colors and beautiful flower shapes, and is widely loved by the people. "Compendium of Materia Medica" said: "Hemerocallis is originally made of stalks. Scallops, forgetting." The ancients thought that daylilies could make people forget their worries, so they called them "Forget Worry Grass". "Fengtuji" records: "'Pregnant women who wear the grass will give birth to boys', so...

Examples

Embodiment 1

[0054] In mid-June 2014, the anthers of the diploid Hemerocallis species Double Firecracker and Golden Baby were selected for flower cultivation to induce detoxification, and the detailed steps are as follows:

[0055] (1) Selection of flower buds to be inoculated

[0056] On June 25th, when the double-petalled firecrackers and golden dolls were budding and blooming, most of the anthers were in the late mononucleate flower buds, and they were brought back to the laboratory with a curling bottle, wrapped in moist gauze, and placed in a refrigerator at 4°C for low temperature pretreatment. Process 4d;

[0057] (2) Preparation of Hemerocallis anther induction medium

[0058] Hemerocallis anther induction medium is prepared from the following substances per liter of distilled water:

[0059] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ∙7H 2 O 370mg / L, CaCl 2 ∙2H 2 O 840mg / L;

[0060] Trace elements: MnSO 4 ∙4H 2 O 22.5mg / L, ZnSO 4...

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