Selenium-enriched Cordyceps fungus and cultivation method thereof, selenium-enriched rice wine and preparation method thereof

Abstract

The invention provides a culture method of selenium-enriched cordyceps sinensis. According to the culture method, selenium-enriched culture of cordyceps sinensis is carried out by adopting a manner of adding inorganic selenium twice in the fermentation culture process of cordyceps sinensis, by defining the two-time adding time and adding amount of inorganic selenium, the limiting bottleneck of the toxicity of inorganic selenium for the selenium amount is solved, thus the selenium content of cordyceps sinensis is obviously improved, and therefore, organic selenium enriched cordyceps sinensis is obtained. In addition, the intention further provides a preparation method of selenium-enriched yellow rice wine; according to the preparation method of the selenium-enriched yellow rice wine, organic selenium enriched cordyceps sinensis is compounded with Chinese-dates and Chinese wolfberry fruits to prepare compound cordyceps sinensis nutrient solution, the obtained compound cordyceps sinensis nutrient solution is mixed with cooked milled glutinous broomcorn millets, through the traditional brewing technology of yellow rice wine, the selenium-enriched cordyceps sinensis health wine which is orange-yellow in color, is clear and transparent, has gloss, and has coordinated aroma is brewed, and the obtained wine body is full and coordinated, mellow and refreshing, has the typical aroma of yellow rice wine and cordyceps sinensis, and also has the nutrition and efficacies of cordyceps sinensis and organic selenium, thus being the novel selenium-enriched yellow rice wine with the health care function.

Description

technical field

[0001] The invention relates to the technical field of selenium enrichment, in particular to a selenium-enriched Cordyceps fungus and a method for cultivating the selenium-enriched Cordyceps fungus, and also relates to a selenium-enriched rice wine prepared from the selenium-enriched Cordyceps fungus and a preparation method. Background technique

[0002] Selenium (Se) is one of the trace elements necessary for human and animal life activities. It exists mostly in the liver, kidney, pancreas, heart, spleen, tooth enamel and nails of the human body. Selenium exists in nature as inorganic selenium and organic selenium. Inorganic selenium is highly toxic and has certain toxic effects on animal, plant and microbial cells. Therefore, the selenium usually supplemented in food exists in the form of organic selenium. At present, organic selenium is mostly obtained by biotransformation.

[0003] Cordyceps sinensis (Cordyceps sinensis), also known as Chinese caterpilla...

Examples

Embodiment 1

[0031] This embodiment relates to selenium-enriched Cordyceps fungus and its cultivation method.

Embodiment 11

[0033] This embodiment relates to the cultivation of a selenium-enriched Cordyceps fungus, comprising the following steps:

[0034] A, slant culture Cordyceps: prepare potato agar medium as the slant medium of Cordyceps, the slant medium components are potato powder 6.0g / L, glucose 20.0g / L and agar 20.0g / L; Cordyceps strains were inoculated on the slant medium, cultured at 32-35°C for 48 hours, and then stored in a 4°C refrigerator to obtain the Cordyceps slant;

[0035] b. Cultivate Cordyceps seed liquid: prepare seed medium, the components are glucose 40g / L, peptone 10.0g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L; take Cordyceps under aseptic operation The slope is 0.5cm 3 The inoculation amount per L was inoculated into the seed medium, and cultured statically at 32-35°C for 3 days to obtain the Cordyceps seed liquid;

[0036] c. Carry out fermentation and cultivation of Cordyceps seed liquid: prepare fermentation medium, the components are glucos...

Embodiment 12

[0038] This embodiment relates to the cultivation of a selenium-enriched Cordyceps fungus, comprising the following steps:

[0039] A, slant culture Cordyceps: prepare potato agar medium as the slant medium of Cordyceps, the slant medium components are potato powder 6.0g / L, glucose 20.0g / L and agar 20.0g / L; Cordyceps strains were inoculated on the slant medium, cultured at 32-35° C. for 48 hours, and then stored in a 4° C. refrigerator to obtain the Cordyceps slant;

[0040] b. Cultivate Cordyceps seed solution: prepare seed medium, the components are glucose 40g / L, peptone 12.0g / L, potassium dihydrogen phosphate 0.7g / L, magnesium sulfate 0.7g / L; take Cordyceps under aseptic operation The slope is 0.5cm 3 The inoculation amount per L was inoculated into the seed medium, and cultured statically at 32-35°C for 3 days to obtain the Cordyceps seed liquid;

[0041] c. Carry out fermentation and cultivation of Cordyceps seed liquid: prepare fermentation medium, the components are ...

technical field

[0001] The invention relates to the technical field of selenium enrichment, in particular to a selenium-enriched Cordyceps fungus and a method for cultivating the selenium-enriched Cordyceps fungus, and also relates to a selenium-enriched rice wine prepared from the selenium-enriched Cordyceps fungus and a preparation method. Background technique

[0002] Selenium (Se) is one of the trace elements necessary for human and animal life activities. It exists mostly in the liver, kidney, pancreas, heart, spleen, tooth enamel and nails of the human body. Selenium exists in nature as inorganic selenium and organic selenium. Inorganic selenium is highly toxic and has certain toxic effects on animal, plant and microbial cells. Therefore, the selenium usually supplemented in food exists in the form of organic selenium. At present, organic selenium is mostly obtained by biotransformation.

[0003] Cordyceps sinensis (Cordyceps sinensis), also known as Chinese caterpilla...

Examples

Embodiment 1

[0031] This embodiment relates to selenium-enriched Cordyceps fungus and its cultivation method.

Embodiment 11

[0033] This embodiment relates to the cultivation of a selenium-enriched Cordyceps fungus, comprising the following steps:

[0034] A, slant culture Cordyceps: prepare potato agar medium as the slant medium of Cordyceps, the slant medium components are potato powder 6.0g / L, glucose 20.0g / L and agar 20.0g / L; Cordyceps strains were inoculated on the slant medium, cultured at 32-35°C for 48 hours, and then stored in a 4°C refrigerator to obtain the Cordyceps slant;

[0035] b. Cultivate Cordyceps seed liquid: prepare seed medium, the components are glucose 40g / L, peptone 10.0g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L; take Cordyceps under aseptic operation The slope is 0.5cm 3 The inoculation amount per L was inoculated into the seed medium, and cultured statically at 32-35°C for 3 days to obtain the Cordyceps seed liquid;

[0036] c. Carry out fermentation and cultivation of Cordyceps seed liquid: prepare fermentation medium, the components are glucos...

Embodiment 12

[0038] This embodiment relates to the cultivation of a selenium-enriched Cordyceps fungus, comprising the following steps:

[0039] A, slant culture Cordyceps: prepare potato agar medium as the slant medium of Cordyceps, the slant medium components are potato powder 6.0g / L, glucose 20.0g / L and agar 20.0g / L; Cordyceps strains were inoculated on the slant medium, cultured at 32-35° C. for 48 hours, and then stored in a 4° C. refrigerator to obtain the Cordyceps slant;

[0040] b. Cultivate Cordyceps seed solution: prepare seed medium, the components are glucose 40g / L, peptone 12.0g / L, potassium dihydrogen phosphate 0.7g / L, magnesium sulfate 0.7g / L; take Cordyceps under aseptic operation The slope is 0.5cm 3 The inoculation amount per L was inoculated into the seed medium, and cultured statically at 32-35°C for 3 days to obtain the Cordyceps seed liquid;

[0041] c. Carry out fermentation and cultivation of Cordyceps seed liquid: prepare fermentation medium, the components are ...