Synergistic method of citrus naringenin and high-intensity pulsed electric field to kill Staphylococcus aureus

A technology of pulsed electric field and staphylococcus, which is applied in the direction of milk preservation, food electrical treatment, food science, etc., can solve the problems of increasing equipment load, increasing processing time, reducing the service life of components, etc., to reduce energy input and fence Increase the factor to solve the effect of large energy consumption

CN107348305BActive Publication Date: 2020-12-22SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-12-22

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Abstract

The invention discloses a method for synergistically killing staphylococcus aureus through citruse naringenin and a high-strength pulsed electric field. The method comprises the following steps of adding naringenin to a liquid food containing staphylococcus aureus, to be sterilized, and performing pretreatment; and preforming sterilization on materials pretreated with the naringenin by the high-strength pulsed electric field. Preferably, the addition concentration of the naringenin is 12.5-125mg / L, the pretreatment time is 0.5-1.0h, the parameters of the pulsed electric field are set as that the electric field strength is 20-50kV / cm, the pulse width is 10-40[mu]s, the pulse frequency is 0.1-1.0kHz, and the total time that materials are subjected to pulsing treatment is 60-1000[mu]s. According to the method disclosed by the invention, a natural antibacterial ingredient namely the naringenin is combined with a high-strength pulsed electric field sterilization technique, so that the effect of killing the staphylococcus aureus is notably increased, the food is safe, the storage period is prolonged, and the loss of original flavor and original nutrient substances is reduced.
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Description

Technical field

[0001] The invention relates to the field of food sterilization, in particular to a method for synergistically killing Staphylococcus aureus based on citrus naringenin and a high-strength pulsed electric field.Background technique

[0002] Staphylococcus aureus is a common gram-positive bacterium that is ubiquitous in nature. It can be found in air, water, dust, and human and animal excreta. There are many opportunities for food to be contaminated. In addition, Staphylococcus aureus has strong viability, good salt tolerance and drying resistance. It can multiply in meat products, dairy products, beverages and other foods to produce enterotoxins, which can cause nausea, vomiting, and diarrhea among consumers. The US Centers for Disease Control report pointed out that food-borne diseases caused by Staphylococcus aureus occupy the second place in the world, second only to E. coli. In addition to producing enteric toxins and causing food poisoning, Staphylococcus aureus is als...

Examples

Embodiment 1

[0033]Fresh orange juice is inoculated with Staphylococcus aureus to make the colony count to 108~109CFU / mL order of magnitude; for pulsed electric field treatment, the parameters for pulsed electric field treatment are: electric field intensity 45.0kV / cm, pulse width 40μs, frequency 1.0kHz, time 500μs;

[0034]According to the concentration in orange juice, the usage amount of naringenin is 50.0mg / L (extracted from grapefruit peel, purity is about 95%).

[0035]Treatment method: After pretreatment with naringenin for 1.0h, pulse sterilization is performed.

[0036]To illustrate the effect of the method of the present invention, this embodiment compares the sterilization effect of the method of the present invention with the following methods:

[0037]Treatment conditions: pulsed electric field intensity of 0, 15.0, 30.0 and 45.0kV / cm respectively; without naringenin, treatment for 500μs;

[0038]Treatment conditions: pulsed electric field intensity was 0, 15.0, 30.0 and 45.0 kV / cm, plus naringeni...

Embodiment 2

[0042]Fresh mango juice is inoculated with Staphylococcus aureus to make the number of colonies reach 108~109CFU / mL, add naringenin; pulse electric field treatment immediately, the electric parameters of pulse electric field treatment are: electric field strength 20.0kV / cm, pulse width 40μs, frequency 0.1kHz, time 1000μs;

[0043]According to the concentration in mango juice, the usage amount of naringenin is 125.0mg / L (extracted from grapefruit peel, purity is about 95%).

[0044]Treatment method: After pretreatment with naringenin for 1.0h, pulse sterilization is performed.

[0045]To illustrate the effect of the method of the present invention, this embodiment compares the sterilization effect of the method of the present invention with the following methods:

[0046]Treatment conditions: pulsed electric field intensity is 0, naringenin concentration is 125.0mg / L; pulsed electric field intensity is 20.0kV / cm; without naringenin, treatment is 1000μs;

[0047]The number of colonies of Staphylococ...

Embodiment 3

[0050]Inoculate fresh milk with Staphylococcus aureus to reach 108~109CFU / mL order of magnitude; pulse electric field treatment immediately or add a certain concentration of naringenin, the electric parameters of pulse electric field treatment are: electric field intensity 50.0kV / cm, pulse width 10μs, frequency 1.0kHz, time 60μs;

[0051]According to the concentration in milk, the use amount of naringenin is 12.5mg / L (extracted from grapefruit peel, purity is about 95%).

[0052]Treatment method: After pretreatment with naringenin for 0.5h, pulse sterilization is performed.

[0053]To illustrate the effect of the method of the present invention, this embodiment compares the sterilization effect of the method of the present invention with the following methods:

[0054]Treatment conditions: pulsed electric field intensity is 0, naringenin concentration is 12.5mg / L; pulsed electric field intensity is 50.0kV / cm; without naringenin, treatment 60μs;

[0055]The number of colonies of Staphylococcus aure...