A magnetic enzyme immunofluorescence quantitative analyzer

Abstract

The invention discloses a magnetic enzyme fluorescence quantitative analyzer, comprising a bottom plate, on which a plurality of pipetting mechanisms with the same structure are arranged, the pipetting mechanism comprising a support frame, on which a front-and-back movement component is arranged, on which a board and card placement frame is arranged, on which an up-and-down movement component is arranged, on which a mounting block is arranged, on which a liquid suction piston is fixed, a detection drive component is arranged at the rear end of the pipetting mechanism, and on which a detection mechanism is arranged; the board and card placement frame moves to a pipetting station, the liquid suction piston puts the sucked magnetic bead test solution into a test solution tank of the board and card, and then the board and card placement frame moves backward to the side of the detection mechanism, so that the test solution tank is located below the detection mechanism, and the detection is automatically completed by using the detection mechanism, so that the entire reaction process is more intelligent, manual participation is reduced, and the detection accuracy and efficiency are improved.

Description

Technical Field

[0001] The invention relates to the field of chemical instruments and equipment, in particular to a magnetic enzyme-free fluorescence quantitative analyzer. Background Art

[0002] The magnetic enzyme-free fluorescence quantitative analyzer is an instrument that uses a luminescence system and immunoassay methods to perform quantitative detection of various analytes in serum, plasma or other body fluids. The reagent strip contains test solution and magnetic beads. Magnetic beads are magnetic solid-phase microparticles of a certain particle size synthesized using polymer materials. The magnetic beads are used as carriers to coat various immunoactive substances with specific affinity to sensitize them into immunomagnetic particles. The test solution is mixed and cultured with the magnetic beads in the reagent strip, and then the test solution is observed using the detection system to obtain the test results. Ordinary fluorescence reaction instruments on the market mix the magnetic bead test solution and the test solution for color observation or microscopic detection. They cannot automatically mix the test solution with the magnetic bead test solution. The previous steps still need to be manually operated, which makes the detection rate and detection accuracy very low. Summary of the invention

[0003] In order to overcome the above-mentioned deficiencies of the prior art, the present invention provides a magnetic enzyme fluorescence quantitative analyzer which can automatically mix the liquid to be detected with the magnetic bead test solution and automatically detect and observe the mixed solution, thereby greatly improving the processing efficiency and accuracy.

[0004] In order to achieve the above object, the technical solution adopted by the present invention is:

[0005] A magnetic enzyme-free fluorescence quantitative analyzer comprises a bottom plate, on which a plurality of pipetting mechanisms with the same structure are arranged, the pipetting mechanisms comprising a support frame, on which a front-and-rear motion component is provided, on which a board placement frame is provided, on which an up-and-down motion component is provided, on which a mounting block is provided, on which a liquid suction piston is fixed, a tip seat is provided at the lower end of the liquid suction piston, in which a tip connected to the liquid suction piston is provided, a detection drive component is provided at the rear end of the pipetting mechanism, and a detection mechanism is provided on the detection drive component.

[0006] As a further improvement of the above scheme, a rotating motor is arranged on the support frame, the main shaft of the rotating motor is hinged with a connecting rod through a transmission mechanism, a magnet is arranged at the end of the connecting rod, and the rotating motor drives the connecting rod to rotate around the hinge point so that the magnet moves away from or close to the board placement frame.

[0007] As a further improvement of the above solution, the transmission mechanism is a gear set, which includes a first gear sleeved on the rotating motor, a second gear hinged to the connecting rod, and a transmission gear connecting the first gear and the second gear.

[0008] As a further improvement of the above solution, the up and down motion component includes a vertical slide rail, a vertical slider is provided on the vertical slide rail, the mounting block is fixed on the vertical slider, and the mounting block is connected to a first drive motor.

[0009] As a further improvement of the above solution, the forward and backward motion component includes a horizontal slide rail, a horizontal slider is provided on the horizontal slide rail, the board and card placement rack is fixed on the horizontal slider, and the board and card placement rack is connected to a second driving motor.

[0010] As a further improvement of the above scheme, the detection mechanism is movably arranged on a mounting seat on the detection drive assembly, a detection hole is arranged on the mounting seat, a light emitting channel is connected below the detection hole, a xenon lamp for emitting light is arranged in the light emitting channel, a photomultiplier tube for receiving light is arranged next to the detection hole, a detection lens assembly is arranged at the front end of the photomultiplier tube, a detection scanner is fixed above the mounting seat, and the detection scanner is located above the detection hole.

[0011] As a further improvement of the above solution, the detection lens assembly includes a lens base, a convex lens, a first filter and a collection photodiode arranged in the lens base from front to back.

[0012] As a further improvement of the above scheme, a light source detection channel is also connected below the detection hole, and a second filter and a detection photodiode arranged front to back are arranged in the light source detection channel, and the light source detection channel is perpendicular to the light emission channel.

[0013] As a further improvement of the above solution, a standard substrate fixing seat is fixed beside the pipetting mechanism, a standard substrate board is arranged in the standard substrate fixing seat, and the standard substrate fixing seat and the board placement rack are located at the same height.

[0014] As a further improvement of the above solution, a heating device and a temperature controller are arranged on the bottom plate, a board heating plate is arranged in the board placement rack, and a test liquid heating plate is arranged in the suction head holder.

[0015] Beneficial effects of the present invention:

[0016] The invention discloses a magnetic enzyme fluorescence quantitative analyzer. A board card is placed on a board card placement rack. A liquid suction piston is started to suck up a magnetic bead test solution in the board card. The board card placement rack moves to a liquid transfer station. The liquid suction piston puts the sucked magnetic bead test solution into a test solution tank of the board card. Then, the board card placement rack moves backward to the side of a detection mechanism, so that the test solution tank is located below the detection mechanism. The detection is automatically completed by using the detection mechanism. The entire reaction process is more intelligent, manual participation is reduced, and the detection accuracy and efficiency are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following is a brief description of the drawings required for describing the embodiments. Obviously, the drawings described are only part of the embodiments of the present invention, not all of them. Those skilled in the art can also obtain other design solutions and drawings based on these drawings without creative work:

[0018] Figure 1 It is a schematic diagram of the structure of a preferred embodiment of the present invention;

[0019] Figure 2 It is a schematic diagram of the structure of a preferred embodiment of the present invention;

[0020] Figure 3 It is a structural schematic diagram of the liquid transfer mechanism and the detection mechanism of a preferred embodiment of the present invention;

[0021] Figure 4 It is a structural schematic diagram of the liquid transfer mechanism of a preferred embodiment of the present invention;

[0022] Figure 5 It is a structural schematic diagram of the liquid transfer mechanism of a preferred embodiment of the present invention;

[0023] Figure 6 It is a structural schematic diagram of the liquid transfer mechanism of a preferred embodiment of the present invention;

[0024] Figure 7 This is a front view of the detection mechanism of a preferred embodiment of the present invention;

[0025] Figure 8 It is a structural schematic diagram of a detection mechanism of a preferred embodiment of the present invention;

[0026] Fig. 9 for Figure 7 Cross-sectional view along the AA direction. DETAILED DESCRIPTION

[0027] The following will be combined with the embodiments and drawings to clearly and completely describe the concept, specific structure and technical effects of the present invention, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0028] Reference Figures 1 to 9A magnetic enzyme-free fluorescence quantitative analyzer comprises a base plate 1, on which a plurality of pipetting mechanisms 2 with the same structure are arranged, the pipetting mechanism 2 comprises a support frame 21, on which a forward and backward movement component 8 is arranged, on which a board placement frame 22 is arranged, on which an up and down movement component is arranged, on which a mounting block 23 is arranged on the up and down movement component 7, on which a liquid suction piston 24 is fixed, a suction head seat 25 is arranged at the lower end of the liquid suction piston 24, in which a suction head 26 connected to the liquid suction piston 24 is arranged, a detection drive component 9 is arranged at the rear end of the pipetting mechanism 2, and a detection mechanism 3 is arranged on the detection drive component 9.

[0029] Reference Figures 3 to 6 The up-and-down motion assembly 7 includes a vertical slide rail 71, a vertical slider is provided on the vertical slide rail 71, the mounting block 23 is fixed on the vertical slider, and the mounting block 23 is connected to a first drive motor 72. The forward-and-backward motion assembly 8 includes a horizontal slide rail 81, a horizontal slider 82 is provided on the horizontal slide rail 81, the board placement rack 22 is fixed on the horizontal slider 82, and the board placement rack 22 is connected to a second drive motor 83.

[0030] The mounting block 23 moves up and down along the vertical slide rail 71. When aspirating liquid, the mounting block 23 moves downward, so that the suction head 26 in the suction head seat 25 extends downward into the test liquid slot of the board card, and then the piston block of the suction piston 24 moves upward, and the suction head 26 extracts the test liquid upward to complete the aspiration. After the aspiration is completed, the mounting block 23 moves upward to facilitate the backward movement of the board card, so that the empty slot in the board card is under the suction head 26, and then the piston block of the suction piston 24 moves downward, so that the test liquid in the suction head 26 is discharged into the empty slot. The entire aspiration and discharge process is completed automatically, and the processing efficiency is greatly improved.

[0031] The support frame 21 is provided with a rotating motor 27, the main shaft of the rotating motor 27 is hinged with a connecting rod 28 through a transmission mechanism 6, the end of the connecting rod 28 is provided with a magnet 29, and the rotating motor 27 drives the connecting rod 28 to rotate around the hinge point, so that the magnet 29 is away from or close to the board placement frame 22. The transmission mechanism 6 is a gear set, which includes a first gear 61 sleeved on the rotating motor 27, a second gear 62 hinged with the connecting rod 28, and a transmission gear 63 connecting the first gear 61 and the second gear 62.

[0032] When aspirating liquid, the connecting rod 28 drives the magnet 29 to approach the board and card placement rack 22, so that the magnetic beads in the board and card move and gather, so that the magnetic beads deposited in the board and card move and stir, so that the magnetic bead reagent is more uniform, and it is convenient for the suction head 26 to absorb the magnetic bead test liquid. When the absorbed test liquid is discharged into the detection slot of the board and card, the connecting rod 28 drives the magnet 29 away from the board and card placement rack 22, so that the magnetic beads are reset. The structural design is ingenious, which greatly improves the efficiency of aspiration and transfer.

[0033] Reference Figures 7 to 9 The detection mechanism 3 is movably arranged on a mounting seat 31 on the detection driving assembly, a detection hole 32 is arranged on the mounting seat 31, a light emitting channel 33 is connected below the detection hole 32, a xenon lamp 34 for emitting light is arranged in the light emitting channel 33, a photomultiplier tube 35 for receiving light is arranged next to the detection hole 32, a detection lens assembly is arranged at the front end of the photomultiplier tube 35, and the detection lens assembly includes a lens base 310, a convex lens 311, a first filter 312 and a collection photodiode 313 arranged in the lens base 310 from front to back.

[0034] A detection scanner 36 is fixed above the mounting seat 31 , and the detection scanner 36 is located above the detection hole 32 . The detection scanner 36 reads and scans the barcode on the board, can distinguish the test solution information, and stores it together with the photoelectric signal for subsequent data processing.

[0035] The detection drive assembly 9 includes a detection rail 91, on which a detection slider 92 is arranged, and the detection slider 92 is connected to a linear drive device. The mounting seat 31 is fixed on the detection slider 92. The linear drive device can be selected from different linear motion mechanisms such as a linear motor, a screw and a screw nut, and a driving pulley.

[0036] The xenon lamp 34 emits xenon light into the detection slot of the detection board, and the xenon light is emitted from the detection slot. The convex lens 311 collects the light, and the first filter 312 filters the ambient light to prevent stray light from affecting the results and improve the detection accuracy. The light signal of the light is collected by the photodiode 313. Then the photomultiplier tube 35 converts the light signal into an electrical signal for transmission, and gives the relative intensity of fluorescence according to the strength of the electrical signal, and then analyzes the concentration of PCT, and finally calculates the result, realizing the automatic analysis and detection of the test solution, which greatly improves the detection rate and accuracy.

[0037] A light source detection channel 37 is also connected below the detection hole 32. A second filter 38 and a detection photodiode 39 arranged front to back are provided in the light source detection channel 37. The light source detection channel 37 is perpendicular to the light emitting channel 33. The light source detection channel 37 detects the intensity of the emitted light to ensure that the intensity of the emitted light is consistent, maintains qualitativeness during light detection, and improves detection accuracy.

[0038] A standard substrate fixing seat 4 is fixed next to the pipetting mechanism 2, and a standard substrate board 5 is arranged in the standard substrate fixing seat 4. The standard substrate fixing seat 4 and the board placement rack 22 are located at the same height. Before the detection starts, the detection mechanism 3 moves to the bottom of the standard substrate fixing seat 4 to play a comparison role in the initial position, ensuring that the xenon lamp 34 and the photomultiplier tube 35 can emit and receive light, thereby playing a role in positioning and adjustment.

[0039] A heating device 10 and a temperature controller 11 are provided on the bottom plate 1. The heating device heats the entire interior of the device. A board heating plate is provided in the board placement rack 22, and a test liquid heating plate 210 is provided in the tip holder 25, so that the test liquid is always at a constant temperature during the processing and will not cool down. The temperature controller 11 is used to detect the temperature inside the device to ensure that the reaction temperature is within an ideal range and will not be too high or too low, so that the reaction result of the test liquid is more reliable.

[0040] The above is a specific description of the preferred embodiments of the present invention, but the invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the present invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A magnetic enzyme immunofluorescence quantitative analyzer, comprising a bottom plate (1), characterized in that: A plurality of pipetting mechanisms (2) of the same structure are arranged on the bottom plate (1), the pipetting mechanism (2) comprising a support frame (21), a front-and-rear motion component (8) being arranged on the support frame (21), a board placement frame (22) being arranged on the front-and-rear motion component (8), an up-and-down motion component (7) being arranged on the up-and-down motion component (7), a mounting block (23) being arranged on the mounting block (23), a liquid suction piston (24) being fixed on the mounting block (23), a suction head seat (25) being arranged at the lower end of the liquid suction piston (24), a suction head (26) connected to the liquid suction piston (24) being arranged in the suction head seat (25), a detection drive component (9) being arranged at the rear end of the pipetting mechanism (2), and a detection mechanism (3) being arranged on the detection drive component (9); The support frame (21) is provided with a rotating motor (27), the main shaft of the rotating motor (27) is hingedly connected to a connecting rod (28) through a transmission mechanism (6), a magnet (29) is provided at the end of the connecting rod (28), and the rotating motor (27) drives the connecting rod (28) to rotate around the hinge point, so that the magnet (29) moves away from or close to the board placement frame (22); The detection mechanism (3) comprises a mounting seat (31) movably mounted on a detection drive assembly (9); a detection hole (32) is arranged on the mounting seat (31); a light emitting channel (33) is connected below the detection hole (32); a xenon lamp (34) for emitting light is arranged in the light emitting channel (33); a photomultiplier tube (35) for receiving light is arranged next to the detection hole (32); a detection lens assembly is arranged at the front end of the photomultiplier tube (35); a detection scanner (36) is fixed above the mounting seat (31); the detection scanner (36) is located above the detection hole (32).

2. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: The transmission mechanism (6) is a gear set, comprising a first gear (61) sleeved on the rotating motor (27), a second gear (62) hingedly connected to the connecting rod (28), and a transmission gear (63) connecting the first gear (61) and the second gear (62).

3. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: The up-and-down motion assembly (7) comprises a vertical slide rail (71), a vertical slide block is provided on the vertical slide rail (71), the mounting block (23) is fixed on the vertical slide block, and the mounting block (23) is connected to a first drive motor (72).

4. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: The forward and backward motion component (8) comprises a horizontal slide rail (81), a horizontal slide block (82) is provided on the horizontal slide rail (81), the board placement rack (22) is fixed on the horizontal slide block (82), and the board placement rack (22) is connected to a second drive motor (83).

5. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: The detection lens assembly comprises a lens base (310), a convex lens (311), a first filter (312), and a collection photodiode (313) which are arranged inside the lens base (310) from front to back.

6. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: A light source detection channel (37) is also connected below the detection hole (32), and a second filter (38) and a detection photodiode (39) arranged in a front-to-rear manner are provided in the light source detection channel (37), and the light source detection channel (37) and the light emission channel (33) are perpendicular to each other.

7. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: A standard substrate fixing seat (4) is fixed next to the liquid transfer mechanism (2), a standard substrate board (5) is arranged inside the standard substrate fixing seat (4), and the standard substrate fixing seat (4) and the board placement rack (22) are located at the same height.

8. The magnetic enzyme immunofluorescence quantitative analyzer according to claim 1, characterized in that: The bottom plate (1) is provided with a heating device (10) and a temperature controller (11), the board and card placement rack (22) is provided with a board and card heating plate, and the suction head seat (25) is provided with a test liquid heating plate (210).

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